Dio1 cDNA ORF clone, Mus musculus(house mouse)
| Gene Symbol | Dio1 |
| Entrez Gene ID | 13370 |
| Full Name | deiodinase, iodothyronine, type I |
| Synonyms | 5DI,D1,ITDI1,TXDI1 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Mus musculus(house mouse) |
| Genome | |
| Summary | The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the activation, as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. The activation reaction involves the conversion of the prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4), secreted by the thyroid gland, to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by 5'-deiodination. This protein is expressed predominantly in the liver and kidney and provides most of the circulating T3, which is essential for growth, differentiation and basal metabolism in vertebrates. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Apr 2016]. |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_007860.3 | NP_031886.3 | type I iodothyronine deiodinase |
| NM_007860.4 | NP_031886.3 | type I iodothyronine deiodinase |
| Bos taurus (cattle) | DIO1 | NP_001116065.1 |
| Rattus norvegicus (Norway rat) | Dio1 | NP_067685.5 |
| Gallus gallus (chicken) | DIO1 | NP_001091083.1 |
| Danio rerio (zebrafish) | dio1 | NP_001007284.2 |
| Xenopus tropicalis (tropical clawed frog) | dio1 | NP_001243226.1 |
| Homo sapiens (human) | DIO1 | NP_000783.2 |
| Canis lupus familiaris (dog) | DIO1 | NP_001007127.1 |
| DIO1 | NP_001116124.1 | |
| Mus musculus (house mouse) | Dio1 | NP_031886.3 |
| Pan troglodytes (chimpanzee) | DIO1 | NP_001116123.1 |
Related articles in PubMed
An Essential Physiological Role for MCT8 in Bone in Male Mice.
Leitch VD, Di Cosmo C, Liao XH, O'Boy S, Galliford TM, Evans H, Croucher PI, Boyde A, Dumitrescu A, Weiss RE, Refetoff S, Williams GR, Bassett JHD
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Citterio CE, Veluswamy B, Morgan SJ, Galton VA, Banga JP, Atkins S, Morishita Y, Neumann S, Latif R, Gershengorn MC, Smith TJ, Arvan P
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Type 1 Deiodinase Regulates ApoA-I Gene Expression and ApoA-I Synthesis Independent of Thyroid Hormone Signaling.
Liu J, Hernandez-Ono A, Graham MJ, Galton VA, Ginsberg HN
Arteriosclerosis, thrombosis, and vascular biology36(7)1356-66(2016 Jul)
Perinatal deiodinase 2 expression in hepatocytes defines epigenetic susceptibility to liver steatosis and obesity.
Fonseca TL, Fernandes GW, McAninch EA, Bocco BM, Abdalla SM, Ribeiro MO, Moh?csik P, Fekete C, Li D, Xing X, Wang T, Gereben B, Bianco AC
Proceedings of the National Academy of Sciences of the United States of America112(45)14018-23(2015 Nov)
Characterization of mammalian selenoproteomes.
Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guig? R, Gladyshev VN
Science (New York, N.Y.)300(5624)1439-43(2003 May)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Reductions in Dio1 expression reduce the expression of ApoA-I in a 3,5,3'-triiodothyronine-/thyroid hormone response element-independent manner.
Title: Type 1 Deiodinase Regulates ApoA-I Gene Expression and ApoA-I Synthesis Independent of Thyroid Hormone Signaling.
Within the supportive environment of a research vivarium, mice lacking all three deiodinases can be bred and survive.
Title: Life without the iodothyronine deiodinases.
Systemic changes in thyroid hormone economy as a result of acute food deprivation are not dependent on the deiodinases D1 or D2 but are mediated in part by sequestration of type4 and type3 in tissues and their enhanced metabolism by deiodinase D3.
Title: The 5'-deiodinases are not essential for the fasting-induced decrease in circulating thyroid hormone levels in male mice: possible roles for the type 3 deiodinase and tissue sequestration of hormone.
Results demonstrate changes in D1 activity in white adipose tissue under conditions of changing adiposity, and a stimulatory effect of leptin on D1 activity in the tissue.
Title: Modulation of type I iodothyronine 5'-deiodinase activity in white adipose tissue by nutrition: possible involvement of leptin.
D1 playing an important role in iodide conservation by serving as a scavenger enzyme in peripheral tissues and the thyroid.
Title: Life without thyroxine to 3,5,3'-triiodothyronine conversion: studies in mice devoid of the 5'-deiodinases.
The following Dio1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dio1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu15022 | |
| Clone ID Related Accession (Same CDS sequence) | NM_007860.3 , NM_007860.4 | |
| Accession Version | NM_007860.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-07-09 | |
| Organism | Mus musculus(House mouse) | |
| Product | type I iodothyronine deiodinase | |
| Comment | ||
1 | ATGGGGCTGC CCCAGCTATG GCTGTGGCTG AAGCGGCTTG TGATATTCCT GCAGGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_031886.3 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_007860.3 |
ORF Insert Sequence:
1 | ATGGGGCTGC CCCAGCTATG GCTGTGGCTG AAGCGGCTTG TGATATTCCT GCAGGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu15022 | |
| Clone ID Related Accession (Same CDS sequence) | NM_007860.3 , NM_007860.4 | |
| Accession Version | NM_007860.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-28 | |
| Organism | Mus musculus(house mouse) | |
| Product | type I iodothyronine deiodinase | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645723.11 and BC089608.1. On Apr 26, 2016 this sequence version replaced NM_007860.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC019786.1, U49861.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 8419134 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGGGCTGC CCCAGCTATG GCTGTGGCTG AAGCGGCTTG TGATATTCCT GCAGGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_031886.3 |
| CDS | 51..824 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_007860.4 |
ORF Insert Sequence:
1 | ATGGGGCTGC CCCAGCTATG GCTGTGGCTG AAGCGGCTTG TGATATTCCT GCAGGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
An Essential Physiological Role for MCT8 in Bone in Male Mice. |
|
|
|
Type 1 Deiodinase Regulates ApoA-I Gene Expression and ApoA-I Synthesis Independent of Thyroid Hormone Signaling. |
|
Perinatal deiodinase 2 expression in hepatocytes defines epigenetic susceptibility to liver steatosis and obesity. |
|
Characterization of mammalian selenoproteomes. |