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Eef2k cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Eef2k
Entrez Gene ID 13631
Full Name eukaryotic elongation factor-2 kinase
Synonyms C86191,eEF-2K
General protein information
Preferred Names

eukaryotic elongation factor-2 kinase

Names

eukaryotic elongation factor 2 kinase
calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
eEF-2 kinase
eukaroytic elongation factor 2 kinase
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

7

7|7 F2

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006507344.3 XP_006507407.1 eukaryotic elongation factor 2 kinase isoform X2
XM_006507342.3 XP_006507405.1 eukaryotic elongation factor 2 kinase isoform X1
NM_007908.4 NP_031934.1 eukaryotic elongation factor 2 kinase
XM_006507342.4 XP_006507405.1 eukaryotic elongation factor 2 kinase isoform X1
XM_006507345.4 XP_006507408.1 eukaryotic elongation factor 2 kinase isoform X3
NM_001267710.1 NP_001254639.1 eukaryotic elongation factor 2 kinase
XM_006507343.3 XP_006507406.1 eukaryotic elongation factor 2 kinase isoform X1
XM_006507344.4 XP_006507407.1 eukaryotic elongation factor 2 kinase isoform X2
NM_001267711.1 NP_001254640.1 eukaryotic elongation factor 2 kinase
XM_006507345.3 XP_006507408.1 eukaryotic elongation factor 2 kinase isoform X3
XM_006507346.3 XP_006507409.1 eukaryotic elongation factor 2 kinase isoform X4
XM_006507343.4 XP_006507406.1 eukaryotic elongation factor 2 kinase isoform X1
XM_006507346.4 XP_006507409.1 eukaryotic elongation factor 2 kinase isoform X4

Pathways from BioSystems


Homologies


Homo sapiens (human) EEF2K NP_037434.1
Bos taurus (cattle) EEF2K NP_001179471.1
EEF2K XP_001092565.1
Canis lupus familiaris (dog) EEF2K XP_005621491.1
Pan troglodytes (chimpanzee) EEF2K XP_001161085.2
Rattus norvegicus (Norway rat) Eef2k NP_037079.1
Danio rerio (zebrafish) eef2k NP_001002740.1
Caenorhabditis elegans (roundworm) efk-1 NP_001022572.1
Xenopus tropicalis (tropical clawed frog) eef2k NP_001120146.1
Mus musculus (house mouse) Eef2k NP_001254640.1
Gallus gallus (chicken) EEF2K XP_004945358.1

Gene Ontology


Bibliography

Related articles in PubMed

Direct and indirect activation of eukaryotic elongation factor 2 kinase by AMP-activated protein kinase.
Johanns M, Pyr Dit Ruys S, Houddane A, Vertommen D, Herinckx G, Hue L, Proud CG, Rider MH
Cellular signalling36212-221(2017 Aug)

Selective molecular impairment of spontaneous neurotransmission modulates synaptic efficacy.
Crawford DC, Ramirez DM, Trauterman B, Monteggia LM, Kavalali ET
Nature communications814436(2017 Feb)

eEF2K inhibition blocks A?42 neurotoxicity by promoting an NRF2 antioxidant response.
Jan A, Jansonius B, Delaidelli A, Somasekharan SP, Bhanshali F, Vandal M, Negri GL, Moerman D, MacKenzie I, Calon F, Hayden MR, Taubert S, Sorensen PH
Acta neuropathologica133(1)101-119(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Using an eEF2K gain-of-function design in vitro as well as eEF2K-KO mice, we show that eEF2K activity regulates the excitation/inhibition balance by downregulating vesicle release at inhibitory synapses and tonic inhibition. This translates into a reduced susceptibility of eEF2K-KO mice for seizures without major behavioral alterations with the exception of impaired contextual and trace fear memory
Title: eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures.

eEF2K activity is increased in postmortem Alzheimer's disease (AD) patient cortex and hippocampus, and in the hippocampus of aged transgenic AD mice. eEF2K inhibition using pharmacological or genetic approaches prevented the toxic effects of Abeta42 oligomers on neuronal viability and dendrite formation in vitro. Findings highlight the potential utility of eEF2K inhibition to reduce Abeta-mediated oxidative stress in AD.
Title: eEF2K inhibition blocks A?42 neurotoxicity by promoting an NRF2 antioxidant response.

results suggest that protective versus pro-apoptotic roles of eEF2K depend on the type of cells: eEF2K is protective in highly proliferative cells, such as small intestinal stem cells and cancer cells, which are more susceptible to mitotic catastrophe.
Title: Paradoxical Roles of Elongation Factor-2 Kinase in Stem Cell Survival.

eEF2K protects cancer cells against nutrient starvation by inhibiting protein synthesis rather than by activating autophagy.
Title: Elongation factor 2 kinase promotes cell survival by inhibiting protein synthesis without inducing autophagy.

hese data suggest that mmu-miR-125b decreases NO production in activated macrophages at least partially by suppressing eEF2K and CCNA2 expression.
Title: Mmu-miR-125b overexpression suppresses NO production in activated macrophages by targeting eEF2K and CCNA2.

The following Eef2k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eef2k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu23191 NM_001267710.1
Latest version!		 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OMu23191 NM_007908.4
Latest version!		 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OMu23191 NM_001267711.1
Latest version!		 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OMu23191 XM_006507342.4
Latest version!		 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OMu23191 XM_006507343.4
Latest version!		 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OMu39302 XM_006507344.4
Latest version!		 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OMu39303 XM_006507345.4
Latest version!		 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OMu39304 XM_006507346.4
Latest version!		 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OMu23191 XM_006507342.3 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OMu39302 XM_006507344.3 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OMu39303 XM_006507345.3 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OMu23191 XM_006507343.3 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OMu39304 XM_006507346.3 Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu39303
Clone ID Related Accession (Same CDS sequence) XM_006507345.4 , XM_006507345.3
Accession Version XM_006507345.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1851bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507345.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGAG 
ACAGAGGAGA ACAAGACAAA AGGCTTTGAT TACTTACTGA AGGCGGCAGA AGCTGGTGAC 
AGGCATTCCA TGATTTTAGT GGCCCGAGCT TTTGACACTG GCCTGAACCT CAGCCCAGAC 
AGGTGTCAAG ACTGGTCGGA AGCCTTGCAC TGGTACAACA CAGCCCTGGA GACAACAGAC 
TGCGATGAAG GCGGGGAGTA CGATGGGATA CAGGACGAGC CCCAGTACGC ACTGCTGGCC 
AGGGAGGCGG AGATGCTGCT CACCGGGGGA TTTGGACTGG ACAAGAACCC CCAAAGATCA 
GGAGATTTGT ACACCCAGGC AGCTGAGGCA GCAATGGAAG CCATGAAGGG CCGGCTAGCC 
AACCAGTACT ACGAGAAGGC GGAAGAGGCC TGGGCCCAGA TGGAGGAATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507408.1
CDS349..2199
Translation

Target ORF information:

RefSeq Version XM_006507345.3
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006507345.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGAG 
ACAGAGGAGA ACAAGACAAA AGGCTTTGAT TACTTACTGA AGGCGGCAGA AGCTGGTGAC 
AGGCATTCCA TGATTTTAGT GGCCCGAGCT TTTGACACTG GCCTGAACCT CAGCCCAGAC 
AGGTGTCAAG ACTGGTCGGA AGCCTTGCAC TGGTACAACA CAGCCCTGGA GACAACAGAC 
TGCGATGAAG GCGGGGAGTA CGATGGGATA CAGGACGAGC CCCAGTACGC ACTGCTGGCC 
AGGGAGGCGG AGATGCTGCT CACCGGGGGA TTTGGACTGG ACAAGAACCC CCAAAGATCA 
GGAGATTTGT ACACCCAGGC AGCTGAGGCA GCAATGGAAG CCATGAAGGG CCGGCTAGCC 
AACCAGTACT ACGAGAAGGC GGAAGAGGCC TGGGCCCAGA TGGAGGAATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu39304
Clone ID Related Accession (Same CDS sequence) XM_006507346.3 , XM_006507346.4
Accession Version XM_006507346.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507346.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAGGGAGT GCTTCAGGAC GAAGAAACTC TCCAACTTCT TGCACGCCCA GCAATGGAAG 
GGGGCCTCCA ACTACGTGGC CAAGCGCTAC ATCGAGCCGG TGGACAGGAG CGTGTACTTT 
GAGGATGTGC AGCTCCAGAT GGAGGCGAAG CTCTGGGGGG AGGATTACAA TCGGCACAAG 
CCCCCCAAGC AGGTGGATAT CATGCAGATG TGCATCATTG AGCTAAAGGA CAGACCAGGC 
CAGCCCCTCT TCCACTTGGA GCACTACATT GAGGGCAAGT ACATCAAGTA CAATTCCAAC 
TCAGGCTTTG TCCGTGATGA CAACATCCGA CTAACCCCAC AGGCCTTCAG CCATTTCACA 
TTTGAGCGTT CTGGTCATCA GCTGATTGTA GTGGACATCC AGGGTGTGGG TGACCTTTAT 
ACCGACCCAC AGATCCACAC TGAGAAAGGC ACTGACTTTG GAGATGGTAA CCTTGGTGTC 
CGGGGAATGG CTCTCTTCTT CTACTCTCAT GCCTGCAACC GGATTTGTCA GAGCATGGGC 
CTTACGCCCT TTGACCTCTC CCCACGGGAA CAGGATGCGG TGAATCAGAG CACCAGGCTA 
TTGCAATCAG CCAAGACCAT CTTGAGGGGG ACAGAGGAGA AGTGTGGGAG TCCCCGCATA 
AGGACACTCT CTAGCAGCCG GCCCCCTTTG CTCCTTCGCC TGTCAGAGAA CTCCGGGGAT 
GAGAACATGA GTGACGTGAC CTTTGACTCT CTGCCTTCCT CCCCGTCTTC AGCTACACCA 
CACAGCCAGA AACTGGACCA CCTCCATTGG CCAGTGTTTG GTGACCTCGA TAACATGGGC 
CCTAGAGACC ATGACCGCAT GGACAATCAC CGGGACTCTG AGAATAGTGG GGACAGTGGG 
TATCCAAGCG AGAAGCGAAG TGACCTGGAT GATCCTGAGC CCCGAGAACA CGGCCACTCC 
AACGGCAACC GAAGGCATGA ATCTGACGAG GATAGCCTGG GCAGCTCTGG ACGGGTCTGT 
GTGGAGACGT GGAACCTGCT CAATCCCTCC CGCCTGCACC TGCCGAGGCC CTCGGCCGTG 
GCCCTAGAAG TGCAGAGGCT AAATGCCCTG GACCTTGGAA GGAAAATCGG GAAGTCTGTT 
TTGGGGAAGG TCCATTTGGC CATGGTGCGA TACCACGAGG GCGGGCGCTT CTGCGAGAAG 
GATGAGGAGT GGGATCGAGA GTCAGCCATC TTCCATCTGG AGCATGCAGC TGACCTGGGA 
GAACTGGAGG CCATCGTGGG CCTAGGCCTC ATGTACTCTC AGCTGCCCCA CCACATCCTG 
GCTGATGTCT CTCTGAAGGA GACAGAGGAG AACAAGACAA AAGGCTTTGA TTACTTACTG 
AAGGCGGCAG AAGCTGGTGA CAGGCATTCC ATGATTTTAG TGGCCCGAGC TTTTGACACT 
GGCCTGAACC TCAGCCCAGA CAGGTGTCAA GACTGGTCGG AAGCCTTGCA CTGGTACAAC 
ACAGCCCTGG AGACAACAGA CTGCGATGAA GGCGGGGAGT ACGATGGGAT ACAGGACGAG 
CCCCAGTACG CACTGCTGGC CAGGGAGGCG GAGATGCTGC TCACCGGGGG ATTTGGACTG 
GACAAGAACC CCCAAAGATC AGGAGATTTG TACACCCAGG CAGCTGAGGC AGCAATGGAA 
GCCATGAAGG GCCGGCTAGC CAACCAGTAC TACGAGAAGG CGGAAGAGGC CTGGGCCCAG 
ATGGAGGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507409.1
CDS101..1852
Translation

Target ORF information:

RefSeq Version XM_006507346.3
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006507346.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAGGGAGT GCTTCAGGAC GAAGAAACTC TCCAACTTCT TGCACGCCCA GCAATGGAAG 
GGGGCCTCCA ACTACGTGGC CAAGCGCTAC ATCGAGCCGG TGGACAGGAG CGTGTACTTT 
GAGGATGTGC AGCTCCAGAT GGAGGCGAAG CTCTGGGGGG AGGATTACAA TCGGCACAAG 
CCCCCCAAGC AGGTGGATAT CATGCAGATG TGCATCATTG AGCTAAAGGA CAGACCAGGC 
CAGCCCCTCT TCCACTTGGA GCACTACATT GAGGGCAAGT ACATCAAGTA CAATTCCAAC 
TCAGGCTTTG TCCGTGATGA CAACATCCGA CTAACCCCAC AGGCCTTCAG CCATTTCACA 
TTTGAGCGTT CTGGTCATCA GCTGATTGTA GTGGACATCC AGGGTGTGGG TGACCTTTAT 
ACCGACCCAC AGATCCACAC TGAGAAAGGC ACTGACTTTG GAGATGGTAA CCTTGGTGTC 
CGGGGAATGG CTCTCTTCTT CTACTCTCAT GCCTGCAACC GGATTTGTCA GAGCATGGGC 
CTTACGCCCT TTGACCTCTC CCCACGGGAA CAGGATGCGG TGAATCAGAG CACCAGGCTA 
TTGCAATCAG CCAAGACCAT CTTGAGGGGG ACAGAGGAGA AGTGTGGGAG TCCCCGCATA 
AGGACACTCT CTAGCAGCCG GCCCCCTTTG CTCCTTCGCC TGTCAGAGAA CTCCGGGGAT 
GAGAACATGA GTGACGTGAC CTTTGACTCT CTGCCTTCCT CCCCGTCTTC AGCTACACCA 
CACAGCCAGA AACTGGACCA CCTCCATTGG CCAGTGTTTG GTGACCTCGA TAACATGGGC 
CCTAGAGACC ATGACCGCAT GGACAATCAC CGGGACTCTG AGAATAGTGG GGACAGTGGG 
TATCCAAGCG AGAAGCGAAG TGACCTGGAT GATCCTGAGC CCCGAGAACA CGGCCACTCC 
AACGGCAACC GAAGGCATGA ATCTGACGAG GATAGCCTGG GCAGCTCTGG ACGGGTCTGT 
GTGGAGACGT GGAACCTGCT CAATCCCTCC CGCCTGCACC TGCCGAGGCC CTCGGCCGTG 
GCCCTAGAAG TGCAGAGGCT AAATGCCCTG GACCTTGGAA GGAAAATCGG GAAGTCTGTT 
TTGGGGAAGG TCCATTTGGC CATGGTGCGA TACCACGAGG GCGGGCGCTT CTGCGAGAAG 
GATGAGGAGT GGGATCGAGA GTCAGCCATC TTCCATCTGG AGCATGCAGC TGACCTGGGA 
GAACTGGAGG CCATCGTGGG CCTAGGCCTC ATGTACTCTC AGCTGCCCCA CCACATCCTG 
GCTGATGTCT CTCTGAAGGA GACAGAGGAG AACAAGACAA AAGGCTTTGA TTACTTACTG 
AAGGCGGCAG AAGCTGGTGA CAGGCATTCC ATGATTTTAG TGGCCCGAGC TTTTGACACT 
GGCCTGAACC TCAGCCCAGA CAGGTGTCAA GACTGGTCGG AAGCCTTGCA CTGGTACAAC 
ACAGCCCTGG AGACAACAGA CTGCGATGAA GGCGGGGAGT ACGATGGGAT ACAGGACGAG 
CCCCAGTACG CACTGCTGGC CAGGGAGGCG GAGATGCTGC TCACCGGGGG ATTTGGACTG 
GACAAGAACC CCCAAAGATC AGGAGATTTG TACACCCAGG CAGCTGAGGC AGCAATGGAA 
GCCATGAAGG GCCGGCTAGC CAACCAGTAC TACGAGAAGG CGGAAGAGGC CTGGGCCCAG 
ATGGAGGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu23191
Clone ID Related Accession (Same CDS sequence) XM_006507342.3 , NM_007908.4 , XM_006507342.4 , NM_001267710.1 , XM_006507343.3 , NM_001267711.1 , XM_006507343.4
Accession Version NM_007908.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-27
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122214.4, AK028524.1, AC122248.5 and AI842850.1. On or before Jun 13, 2012 this sequence version replaced NM_001251826.1, NM_007908.3. Transcript Variant: This variant (1) encodes the functional protein. Variants 1, 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.57771.1, BC003433.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_031934.1
CDS347..2521
Translation

Target ORF information:

RefSeq Version NM_007908.4
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_007908.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu23191
Clone ID Related Accession (Same CDS sequence) XM_006507342.3 , NM_007908.4 , XM_006507342.4 , NM_001267710.1 , XM_006507343.3 , NM_001267711.1 , XM_006507343.4
Accession Version NM_001267710.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-27
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK080915.1, AC122248.5 and AI842850.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK080915.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001254639.1
CDS263..2437
Translation

Target ORF information:

RefSeq Version NM_001267710.1
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001267710.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu23191
Clone ID Related Accession (Same CDS sequence) XM_006507342.3 , NM_007908.4 , XM_006507342.4 , NM_001267710.1 , XM_006507343.3 , NM_001267711.1 , XM_006507343.4
Accession Version NM_001267711.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-27
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ052887.1, BC055361.1, AC122248.5 and AI842850.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC055361.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001254640.1
CDS258..2432
Translation

Target ORF information:

RefSeq Version NM_001267711.1
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001267711.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu23191
Clone ID Related Accession (Same CDS sequence) XM_006507342.3 , NM_007908.4 , XM_006507342.4 , NM_001267710.1 , XM_006507343.3 , NM_001267711.1 , XM_006507343.4
Accession Version XM_006507342.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507342.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507405.1
CDS267..2441
Translation

Target ORF information:

RefSeq Version XM_006507342.3
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006507342.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu23191
Clone ID Related Accession (Same CDS sequence) XM_006507342.3 , NM_007908.4 , XM_006507342.4 , NM_001267710.1 , XM_006507343.3 , NM_001267711.1 , XM_006507343.4
Accession Version XM_006507343.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507343.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507406.1
CDS171..2345
Translation

Target ORF information:

RefSeq Version XM_006507343.3
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006507343.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu39302
Clone ID Related Accession (Same CDS sequence) XM_006507344.3 , XM_006507344.4
Accession Version XM_006507344.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507344.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
CATTTGGCCA TGGTGCGATA CCACGAGGGC GGGCGCTTCT GCGAGAAGGA TGAGGAGTGG 
GATCGAGAGT CAGCCATCTT CCATCTGGAG CATGCAGCTG ACCTGGGAGA ACTGGAGGCC 
ATCGTGGGCC TAGGCCTCAT GTACTCTCAG CTGCCCCACC ACATCCTGGC TGATGTCTCT 
CTGAAGGAGA CAGAGGAGAA CAAGACAAAA GGCTTTGATT ACTTACTGAA GGCGGCAGAA 
GCTGGTGACA GGCATTCCAT GATTTTAGTG GCCCGAGCTT TTGACACTGG CCTGAACCTC 
AGCCCAGACA GGTGTCAAGA CTGGTCGGAA GCCTTGCACT GGTACAACAC AGCCCTGGAG 
ACAACAGACT GCGATGAAGG CGGGGAGTAC GATGGGATAC AGGACGAGCC CCAGTACGCA 
CTGCTGGCCA GGGAGGCGGA GATGCTGCTC ACCGGGGGAT TTGGACTGGA CAAGAACCCC 
CAAAGATCAG GAGATTTGTA CACCCAGGCA GCTGAGGCAG CAATGGAAGC CATGAAGGGC 
CGGCTAGCCA ACCAGTACTA CGAGAAGGCG GAAGAGGCCT GGGCCCAGAT GGAGGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507407.1
CDS349..2388
Translation

Target ORF information:

RefSeq Version XM_006507344.3
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006507344.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
CATTTGGCCA TGGTGCGATA CCACGAGGGC GGGCGCTTCT GCGAGAAGGA TGAGGAGTGG 
GATCGAGAGT CAGCCATCTT CCATCTGGAG CATGCAGCTG ACCTGGGAGA ACTGGAGGCC 
ATCGTGGGCC TAGGCCTCAT GTACTCTCAG CTGCCCCACC ACATCCTGGC TGATGTCTCT 
CTGAAGGAGA CAGAGGAGAA CAAGACAAAA GGCTTTGATT ACTTACTGAA GGCGGCAGAA 
GCTGGTGACA GGCATTCCAT GATTTTAGTG GCCCGAGCTT TTGACACTGG CCTGAACCTC 
AGCCCAGACA GGTGTCAAGA CTGGTCGGAA GCCTTGCACT GGTACAACAC AGCCCTGGAG 
ACAACAGACT GCGATGAAGG CGGGGAGTAC GATGGGATAC AGGACGAGCC CCAGTACGCA 
CTGCTGGCCA GGGAGGCGGA GATGCTGCTC ACCGGGGGAT TTGGACTGGA CAAGAACCCC 
CAAAGATCAG GAGATTTGTA CACCCAGGCA GCTGAGGCAG CAATGGAAGC CATGAAGGGC 
CGGCTAGCCA ACCAGTACTA CGAGAAGGCG GAAGAGGCCT GGGCCCAGAT GGAGGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu23191
Clone ID Related Accession (Same CDS sequence) XM_006507342.3 , NM_007908.4 , XM_006507342.4 , NM_001267710.1 , XM_006507343.3 , NM_001267711.1 , XM_006507343.4
Accession Version XM_006507342.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507342.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507405.1
CDS389..2563
Translation

Target ORF information:

RefSeq Version XM_006507342.4
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006507342.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu23191
Clone ID Related Accession (Same CDS sequence) XM_006507342.3 , NM_007908.4 , XM_006507342.4 , NM_001267710.1 , XM_006507343.3 , NM_001267711.1 , XM_006507343.4
Accession Version XM_006507343.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507343.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507406.1
CDS170..2344
Translation

Target ORF information:

RefSeq Version XM_006507343.4
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006507343.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
TGTGTGGAGA CGTGGAACCT GCTCAATCCC TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC 
GTGGCCCTAG AAGTGCAGAG GCTAAATGCC CTGGACCTTG GAAGGAAAAT CGGGAAGTCT 
GTTTTGGGGA AGGTCCATTT GGCCATGGTG CGATACCACG AGGGCGGGCG CTTCTGCGAG 
AAGGATGAGG AGTGGGATCG AGAGTCAGCC ATCTTCCATC TGGAGCATGC AGCTGACCTG 
GGAGAACTGG AGGCCATCGT GGGCCTAGGC CTCATGTACT CTCAGCTGCC CCACCACATC 
CTGGCTGATG TCTCTCTGAA GGAGACAGAG GAGAACAAGA CAAAAGGCTT TGATTACTTA 
CTGAAGGCGG CAGAAGCTGG TGACAGGCAT TCCATGATTT TAGTGGCCCG AGCTTTTGAC 
ACTGGCCTGA ACCTCAGCCC AGACAGGTGT CAAGACTGGT CGGAAGCCTT GCACTGGTAC 
AACACAGCCC TGGAGACAAC AGACTGCGAT GAAGGCGGGG AGTACGATGG GATACAGGAC 
GAGCCCCAGT ACGCACTGCT GGCCAGGGAG GCGGAGATGC TGCTCACCGG GGGATTTGGA 
CTGGACAAGA ACCCCCAAAG ATCAGGAGAT TTGTACACCC AGGCAGCTGA GGCAGCAATG 
GAAGCCATGA AGGGCCGGCT AGCCAACCAG TACTACGAGA AGGCGGAAGA GGCCTGGGCC 
CAGATGGAGG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu39302
Clone ID Related Accession (Same CDS sequence) XM_006507344.3 , XM_006507344.4
Accession Version XM_006507344.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507344.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
CATTTGGCCA TGGTGCGATA CCACGAGGGC GGGCGCTTCT GCGAGAAGGA TGAGGAGTGG 
GATCGAGAGT CAGCCATCTT CCATCTGGAG CATGCAGCTG ACCTGGGAGA ACTGGAGGCC 
ATCGTGGGCC TAGGCCTCAT GTACTCTCAG CTGCCCCACC ACATCCTGGC TGATGTCTCT 
CTGAAGGAGA CAGAGGAGAA CAAGACAAAA GGCTTTGATT ACTTACTGAA GGCGGCAGAA 
GCTGGTGACA GGCATTCCAT GATTTTAGTG GCCCGAGCTT TTGACACTGG CCTGAACCTC 
AGCCCAGACA GGTGTCAAGA CTGGTCGGAA GCCTTGCACT GGTACAACAC AGCCCTGGAG 
ACAACAGACT GCGATGAAGG CGGGGAGTAC GATGGGATAC AGGACGAGCC CCAGTACGCA 
CTGCTGGCCA GGGAGGCGGA GATGCTGCTC ACCGGGGGAT TTGGACTGGA CAAGAACCCC 
CAAAGATCAG GAGATTTGTA CACCCAGGCA GCTGAGGCAG CAATGGAAGC CATGAAGGGC 
CGGCTAGCCA ACCAGTACTA CGAGAAGGCG GAAGAGGCCT GGGCCCAGAT GGAGGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507407.1
CDS536..2575
Translation

Target ORF information:

RefSeq Version XM_006507344.4
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006507344.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGTC 
CATTTGGCCA TGGTGCGATA CCACGAGGGC GGGCGCTTCT GCGAGAAGGA TGAGGAGTGG 
GATCGAGAGT CAGCCATCTT CCATCTGGAG CATGCAGCTG ACCTGGGAGA ACTGGAGGCC 
ATCGTGGGCC TAGGCCTCAT GTACTCTCAG CTGCCCCACC ACATCCTGGC TGATGTCTCT 
CTGAAGGAGA CAGAGGAGAA CAAGACAAAA GGCTTTGATT ACTTACTGAA GGCGGCAGAA 
GCTGGTGACA GGCATTCCAT GATTTTAGTG GCCCGAGCTT TTGACACTGG CCTGAACCTC 
AGCCCAGACA GGTGTCAAGA CTGGTCGGAA GCCTTGCACT GGTACAACAC AGCCCTGGAG 
ACAACAGACT GCGATGAAGG CGGGGAGTAC GATGGGATAC AGGACGAGCC CCAGTACGCA 
CTGCTGGCCA GGGAGGCGGA GATGCTGCTC ACCGGGGGAT TTGGACTGGA CAAGAACCCC 
CAAAGATCAG GAGATTTGTA CACCCAGGCA GCTGAGGCAG CAATGGAAGC CATGAAGGGC 
CGGCTAGCCA ACCAGTACTA CGAGAAGGCG GAAGAGGCCT GGGCCCAGAT GGAGGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu39303
Clone ID Related Accession (Same CDS sequence) XM_006507345.4 , XM_006507345.3
Accession Version XM_006507345.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1851bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507345.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGAG 
ACAGAGGAGA ACAAGACAAA AGGCTTTGAT TACTTACTGA AGGCGGCAGA AGCTGGTGAC 
AGGCATTCCA TGATTTTAGT GGCCCGAGCT TTTGACACTG GCCTGAACCT CAGCCCAGAC 
AGGTGTCAAG ACTGGTCGGA AGCCTTGCAC TGGTACAACA CAGCCCTGGA GACAACAGAC 
TGCGATGAAG GCGGGGAGTA CGATGGGATA CAGGACGAGC CCCAGTACGC ACTGCTGGCC 
AGGGAGGCGG AGATGCTGCT CACCGGGGGA TTTGGACTGG ACAAGAACCC CCAAAGATCA 
GGAGATTTGT ACACCCAGGC AGCTGAGGCA GCAATGGAAG CCATGAAGGG CCGGCTAGCC 
AACCAGTACT ACGAGAAGGC GGAAGAGGCC TGGGCCCAGA TGGAGGAATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507408.1
CDS536..2386
Translation

Target ORF information:

RefSeq Version XM_006507345.4
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006507345.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 
GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 
CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 
AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 
AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 
TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 
GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 
GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 
AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 
TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 
AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 
GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 
AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 
ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 
TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 
GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 
GGCCTTACGC CCTTTGACCT CTCCCCACGG GAACAGGATG CGGTGAATCA GAGCACCAGG 
CTATTGCAAT CAGCCAAGAC CATCTTGAGG GGGACAGAGG AGAAGTGTGG GAGTCCCCGC 
ATAAGGACAC TCTCTAGCAG CCGGCCCCCT TTGCTCCTTC GCCTGTCAGA GAACTCCGGG 
GATGAGAACA TGAGTGACGT GACCTTTGAC TCTCTGCCTT CCTCCCCGTC TTCAGCTACA 
CCACACAGCC AGAAACTGGA CCACCTCCAT TGGCCAGTGT TTGGTGACCT CGATAACATG 
GGCCCTAGAG ACCATGACCG CATGGACAAT CACCGGGACT CTGAGAATAG TGGGGACAGT 
GGGTATCCAA GCGAGAAGCG AAGTGACCTG GATGATCCTG AGCCCCGAGA ACACGGCCAC 
TCCAACGGCA ACCGAAGGCA TGAATCTGAC GAGGATAGCC TGGGCAGCTC TGGACGGGAG 
ACAGAGGAGA ACAAGACAAA AGGCTTTGAT TACTTACTGA AGGCGGCAGA AGCTGGTGAC 
AGGCATTCCA TGATTTTAGT GGCCCGAGCT TTTGACACTG GCCTGAACCT CAGCCCAGAC 
AGGTGTCAAG ACTGGTCGGA AGCCTTGCAC TGGTACAACA CAGCCCTGGA GACAACAGAC 
TGCGATGAAG GCGGGGAGTA CGATGGGATA CAGGACGAGC CCCAGTACGC ACTGCTGGCC 
AGGGAGGCGG AGATGCTGCT CACCGGGGGA TTTGGACTGG ACAAGAACCC CCAAAGATCA 
GGAGATTTGT ACACCCAGGC AGCTGAGGCA GCAATGGAAG CCATGAAGGG CCGGCTAGCC 
AACCAGTACT ACGAGAAGGC GGAAGAGGCC TGGGCCCAGA TGGAGGAATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu39304
Clone ID Related Accession (Same CDS sequence) XM_006507346.3 , XM_006507346.4
Accession Version XM_006507346.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product eukaryotic elongation factor 2 kinase isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507346.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAGGGAGT GCTTCAGGAC GAAGAAACTC TCCAACTTCT TGCACGCCCA GCAATGGAAG 
GGGGCCTCCA ACTACGTGGC CAAGCGCTAC ATCGAGCCGG TGGACAGGAG CGTGTACTTT 
GAGGATGTGC AGCTCCAGAT GGAGGCGAAG CTCTGGGGGG AGGATTACAA TCGGCACAAG 
CCCCCCAAGC AGGTGGATAT CATGCAGATG TGCATCATTG AGCTAAAGGA CAGACCAGGC 
CAGCCCCTCT TCCACTTGGA GCACTACATT GAGGGCAAGT ACATCAAGTA CAATTCCAAC 
TCAGGCTTTG TCCGTGATGA CAACATCCGA CTAACCCCAC AGGCCTTCAG CCATTTCACA 
TTTGAGCGTT CTGGTCATCA GCTGATTGTA GTGGACATCC AGGGTGTGGG TGACCTTTAT 
ACCGACCCAC AGATCCACAC TGAGAAAGGC ACTGACTTTG GAGATGGTAA CCTTGGTGTC 
CGGGGAATGG CTCTCTTCTT CTACTCTCAT GCCTGCAACC GGATTTGTCA GAGCATGGGC 
CTTACGCCCT TTGACCTCTC CCCACGGGAA CAGGATGCGG TGAATCAGAG CACCAGGCTA 
TTGCAATCAG CCAAGACCAT CTTGAGGGGG ACAGAGGAGA AGTGTGGGAG TCCCCGCATA 
AGGACACTCT CTAGCAGCCG GCCCCCTTTG CTCCTTCGCC TGTCAGAGAA CTCCGGGGAT 
GAGAACATGA GTGACGTGAC CTTTGACTCT CTGCCTTCCT CCCCGTCTTC AGCTACACCA 
CACAGCCAGA AACTGGACCA CCTCCATTGG CCAGTGTTTG GTGACCTCGA TAACATGGGC 
CCTAGAGACC ATGACCGCAT GGACAATCAC CGGGACTCTG AGAATAGTGG GGACAGTGGG 
TATCCAAGCG AGAAGCGAAG TGACCTGGAT GATCCTGAGC CCCGAGAACA CGGCCACTCC 
AACGGCAACC GAAGGCATGA ATCTGACGAG GATAGCCTGG GCAGCTCTGG ACGGGTCTGT 
GTGGAGACGT GGAACCTGCT CAATCCCTCC CGCCTGCACC TGCCGAGGCC CTCGGCCGTG 
GCCCTAGAAG TGCAGAGGCT AAATGCCCTG GACCTTGGAA GGAAAATCGG GAAGTCTGTT 
TTGGGGAAGG TCCATTTGGC CATGGTGCGA TACCACGAGG GCGGGCGCTT CTGCGAGAAG 
GATGAGGAGT GGGATCGAGA GTCAGCCATC TTCCATCTGG AGCATGCAGC TGACCTGGGA 
GAACTGGAGG CCATCGTGGG CCTAGGCCTC ATGTACTCTC AGCTGCCCCA CCACATCCTG 
GCTGATGTCT CTCTGAAGGA GACAGAGGAG AACAAGACAA AAGGCTTTGA TTACTTACTG 
AAGGCGGCAG AAGCTGGTGA CAGGCATTCC ATGATTTTAG TGGCCCGAGC TTTTGACACT 
GGCCTGAACC TCAGCCCAGA CAGGTGTCAA GACTGGTCGG AAGCCTTGCA CTGGTACAAC 
ACAGCCCTGG AGACAACAGA CTGCGATGAA GGCGGGGAGT ACGATGGGAT ACAGGACGAG 
CCCCAGTACG CACTGCTGGC CAGGGAGGCG GAGATGCTGC TCACCGGGGG ATTTGGACTG 
GACAAGAACC CCCAAAGATC AGGAGATTTG TACACCCAGG CAGCTGAGGC AGCAATGGAA 
GCCATGAAGG GCCGGCTAGC CAACCAGTAC TACGAGAAGG CGGAAGAGGC CTGGGCCCAG 
ATGGAGGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507409.1
CDS122..1873
Translation

Target ORF information:

RefSeq Version XM_006507346.4
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic elongation factor-2 kinase (Eef2k), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006507346.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAGGGAGT GCTTCAGGAC GAAGAAACTC TCCAACTTCT TGCACGCCCA GCAATGGAAG 
GGGGCCTCCA ACTACGTGGC CAAGCGCTAC ATCGAGCCGG TGGACAGGAG CGTGTACTTT 
GAGGATGTGC AGCTCCAGAT GGAGGCGAAG CTCTGGGGGG AGGATTACAA TCGGCACAAG 
CCCCCCAAGC AGGTGGATAT CATGCAGATG TGCATCATTG AGCTAAAGGA CAGACCAGGC 
CAGCCCCTCT TCCACTTGGA GCACTACATT GAGGGCAAGT ACATCAAGTA CAATTCCAAC 
TCAGGCTTTG TCCGTGATGA CAACATCCGA CTAACCCCAC AGGCCTTCAG CCATTTCACA 
TTTGAGCGTT CTGGTCATCA GCTGATTGTA GTGGACATCC AGGGTGTGGG TGACCTTTAT 
ACCGACCCAC AGATCCACAC TGAGAAAGGC ACTGACTTTG GAGATGGTAA CCTTGGTGTC 
CGGGGAATGG CTCTCTTCTT CTACTCTCAT GCCTGCAACC GGATTTGTCA GAGCATGGGC 
CTTACGCCCT TTGACCTCTC CCCACGGGAA CAGGATGCGG TGAATCAGAG CACCAGGCTA 
TTGCAATCAG CCAAGACCAT CTTGAGGGGG ACAGAGGAGA AGTGTGGGAG TCCCCGCATA 
AGGACACTCT CTAGCAGCCG GCCCCCTTTG CTCCTTCGCC TGTCAGAGAA CTCCGGGGAT 
GAGAACATGA GTGACGTGAC CTTTGACTCT CTGCCTTCCT CCCCGTCTTC AGCTACACCA 
CACAGCCAGA AACTGGACCA CCTCCATTGG CCAGTGTTTG GTGACCTCGA TAACATGGGC 
CCTAGAGACC ATGACCGCAT GGACAATCAC CGGGACTCTG AGAATAGTGG GGACAGTGGG 
TATCCAAGCG AGAAGCGAAG TGACCTGGAT GATCCTGAGC CCCGAGAACA CGGCCACTCC 
AACGGCAACC GAAGGCATGA ATCTGACGAG GATAGCCTGG GCAGCTCTGG ACGGGTCTGT 
GTGGAGACGT GGAACCTGCT CAATCCCTCC CGCCTGCACC TGCCGAGGCC CTCGGCCGTG 
GCCCTAGAAG TGCAGAGGCT AAATGCCCTG GACCTTGGAA GGAAAATCGG GAAGTCTGTT 
TTGGGGAAGG TCCATTTGGC CATGGTGCGA TACCACGAGG GCGGGCGCTT CTGCGAGAAG 
GATGAGGAGT GGGATCGAGA GTCAGCCATC TTCCATCTGG AGCATGCAGC TGACCTGGGA 
GAACTGGAGG CCATCGTGGG CCTAGGCCTC ATGTACTCTC AGCTGCCCCA CCACATCCTG 
GCTGATGTCT CTCTGAAGGA GACAGAGGAG AACAAGACAA AAGGCTTTGA TTACTTACTG 
AAGGCGGCAG AAGCTGGTGA CAGGCATTCC ATGATTTTAG TGGCCCGAGC TTTTGACACT 
GGCCTGAACC TCAGCCCAGA CAGGTGTCAA GACTGGTCGG AAGCCTTGCA CTGGTACAAC 
ACAGCCCTGG AGACAACAGA CTGCGATGAA GGCGGGGAGT ACGATGGGAT ACAGGACGAG 
CCCCAGTACG CACTGCTGGC CAGGGAGGCG GAGATGCTGC TCACCGGGGG ATTTGGACTG 
GACAAGAACC CCCAAAGATC AGGAGATTTG TACACCCAGG CAGCTGAGGC AGCAATGGAA 
GCCATGAAGG GCCGGCTAGC CAACCAGTAC TACGAGAAGG CGGAAGAGGC CTGGGCCCAG 
ATGGAGGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Direct and indirect activation of eukaryotic elongation factor 2 kinase by AMP-activated protein kinase.
Cellular signalling36212-221(2017 Aug)
Johanns M,Pyr Dit Ruys S,Houddane A,Vertommen D,Herinckx G,Hue L,Proud CG,Rider MH


book

Selective molecular impairment of spontaneous neurotransmission modulates synaptic efficacy.
Nature communications814436(2017 Feb)
Crawford DC,Ramirez DM,Trauterman B,Monteggia LM,Kavalali ET


book

eEF2K inhibition blocks A?42 neurotoxicity by promoting an NRF2 antioxidant response.
Acta neuropathologica133(1)101-119(2017 Jan)
Jan A,Jansonius B,Delaidelli A,Somasekharan SP,Bhanshali F,Vandal M,Negri GL,Moerman D,MacKenzie I,Calon F,Hayden MR,Taubert S,Sorensen PH