Gjb3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gjb3
Entrez Gene ID	14620
Full Name	gap junction protein, beta 3
Synonyms	Cnx31,Cx31,D4Wsu144e,Gjb-3
General protein information	Preferred Names gap junction protein, beta 3 Names gap junction beta-3 protein connexin-31 gap junction membrane channel protein beta 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 D2.2\|4 61.48 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001160012.1	NP_001153484.1	gap junction beta-3 protein
NM_008126.2	NP_032152.1	gap junction beta-3 protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GJB3	XP_005629030.1
Danio rerio (zebrafish)	cx35.4	NP_001017685.1
Mus musculus (house mouse)	Gjb3	NP_001153484.1
Xenopus tropicalis (tropical clawed frog)	gjb3	NP_001106371.1
Homo sapiens (human)	GJB3	NP_001005752.1
Gallus gallus (chicken)	GJB3	XP_004947838.1
	GJB3	XP_001108150.1
Bos taurus (cattle)	GJB3	NP_001098465.1
Rattus norvegicus (Norway rat)	Gjb3	NP_062113.1

Gene Ontology
Bibliography

Related articles in PubMed

Increased dosage of the imprinted Ascl2 gene restrains two key endocrine lineages of the mouse Placenta.
Tunster SJ, McNamara GI, Creeth HDJ, John RM
Developmental biology418(1)55-65(2016 10)

Tpbpa-Cre-mediated deletion of TFAP2C leads to deregulation of Cdkn1a, Akt1 and the ERK pathway, causing placental growth arrest.
Sharma N, Kubaczka C, Kaiser S, Nettersheim D, Mughal SS, Riesenberg S, H?lzel M, Winterhager E, Schorle H
Development (Cambridge, England)143(5)787-98(2016 Mar)

The imprinted Phlda2 gene modulates a major endocrine compartment of the placenta to regulate placental demands for maternal resources.
Tunster SJ, Creeth HD, John RM
Developmental biology409(1)251-60(2016 Jan)

Segment-specific expression of the gap junction gene connexin31 during hindbrain development.
Dahl E, Willecke K, Balling R
Development genes and evolution207(5)359-361(1997 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Connexin 31-deficient trophoblast stem cells revealed a shift in giant cell differentiation from Prl3d1 expressing parietal giant cells to Ctsq, Prl3b1, and Prl2c2-positive giant cells.
Title: Connexin 31 (GJB3) deficiency in mouse trophoblast stem cells alters giant cell differentiation and leads to loss of oxygen sensing.

Prostaglandin E stimulates Connexin isoforms via GSK-3beta/beta-catenin via EP2-receptor-dependent cAMP/PKA and PI3K/Akt in mouse embryonic stem cells.
Title: Prostaglandin E? maintains mouse ESC undifferentiated state through regulation of connexin31, connexin43 and connexin45 expression: involvement of glycogen synthase kinase 3?/?-catenin.

Cx31 and Cx43 proteins functionally interact, possibly by forming heteromeric channels in the epidermis.
Title: The connexin31 F137L mutant mouse as a model for the human skin disease erythrokeratodermia variabilis (EKV).

Cx31/Cx43 double-deficient mice exhibit the known phenotypes of the single-knockout strains but no combined effects
Title: Cx31 and Cx43 double-deficient mice reveal independent functions in murine placental and skin development.

During trophoblast cell lineage differentiation, the Cx31 gap junction channel is involved in maintaining the proliferative diploid trophoblast cell population.
Title: Connexin31-deficient trophoblast stem cells: a model to analyze the role of gap junction communication in mouse placental development.

The following Gjb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gjb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01392	NM_001160012.1 Latest version!	Mus musculus gap junction protein, beta 3 (Gjb3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu01392	NM_008126.2 Latest version!	Mus musculus gap junction protein, beta 3 (Gjb3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gjb3 ( NM_001160012.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153484.1 Mus musculus gap junction protein, beta 3 (Gjb3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu01392
Clone ID Related Accession (Same CDS sequence)	NM_001160012.1 , NM_008126.2
Accession Version	NM_001160012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	gap junction beta-3 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ084102.1, BY027476.1, AK083716.1 and X63099.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC024387.1, AK083716.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGACTGGA AGAAGCTCCA AGACCTATTG AGTGGCGTGA ACCAGTACTC CACGGCATTT 
GGGCGCATCT GGCTGTCAGT AGTGTTCGTC TTCCGGGTGC TGGTGTATGT GGTGGCTGCC 
GAGCGTGTGT GGGGTGACGA GCAAAAAGAC TTTGACTGTA ACACCAGGCA ACCCGGCTGT 
ACCAACGTGT GCTATGACAA CTTCTTCCCC ATCTCCAACA TCCGACTCTG GGCCCTGCAG 
CTCATCTTCG TCACGTGTCC GTCTATGCTG GTCATCCTGC ATGTAGCCTA CCGCGAGGAG 
CGGGAACGGA AGCATCGCCA GAAGCACGGG GAGCAATGCG CCAAACTGTA CAGCCACCCG 
GGCAAGAAGC ATGGCGGCCT GTGGTGGACC TACTTGTTTA GCCTCATCTT CAAGCTCATC 
ATCGAATTGG TCTTCCTGTA CGTTCTCCAC ACGCTCTGGC ATGGCTTCAC CATGCCGCGT 
CTGGTACAGT GCGCCAGCAT AGTACCCTGC CCCAACACCG TGGATTGCTA CATCGCTCGG 
CCCACGGAGA AGAAGGTCTT TACCTACTTC ATGGTAGGCG CTTCTGCCGT CTGCATTATT 
CTCACCATCT GTGAGATCTG CTACCTCATC TTCCACAGGA TCATGCGAGG CATAAGCAAG 
GGCAAGTCCA CAAAGAGCAT CAGCTCCCCG AAGTCCTCCA GCCGGGCCTC CACCTGTCGC 
TGTCACCACA AGCTGCTGGA GAGTGGCGAT CCGGAAGCAG ACCCAGCCAG TGAAAAGCTG 
CAGGCTTCAG CGCCCAGCCT GACCCCCATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153484.1
CDS	370..1182
Translation	MDWKKLQDLLSGVNQYSTAFGRIWLSVVFVFRVLVYVVAAERVWGDEQKDFDCNTRQPGCTNVCYDNFFPISNIRLWALQLIFVTCPSMLVILHVAYREERERKHRQKHGEQCAKLYSHPGKKHGGLWWTYLFSLIFKLIIELVFLYVLHTLWHGFTMPRLVQCASIVPCPNTVDCYIARPTEKKVFTYFMVGASAVCIILTICEICYLIFHRIMRGISKGKSTKSISSPKSSSRASTCRCHHKLLESGDPEADPASEKLQASAPSLTPI

Target ORF information:

RefSeq Version	NM_001160012.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, beta 3 (Gjb3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160012.1

ORF Insert Sequence:

ATGGACTGGA AGAAGCTCCA AGACCTATTG AGTGGCGTGA ACCAGTACTC CACGGCATTT 
GGGCGCATCT GGCTGTCAGT AGTGTTCGTC TTCCGGGTGC TGGTGTATGT GGTGGCTGCC 
GAGCGTGTGT GGGGTGACGA GCAAAAAGAC TTTGACTGTA ACACCAGGCA ACCCGGCTGT 
ACCAACGTGT GCTATGACAA CTTCTTCCCC ATCTCCAACA TCCGACTCTG GGCCCTGCAG 
CTCATCTTCG TCACGTGTCC GTCTATGCTG GTCATCCTGC ATGTAGCCTA CCGCGAGGAG 
CGGGAACGGA AGCATCGCCA GAAGCACGGG GAGCAATGCG CCAAACTGTA CAGCCACCCG 
GGCAAGAAGC ATGGCGGCCT GTGGTGGACC TACTTGTTTA GCCTCATCTT CAAGCTCATC 
ATCGAATTGG TCTTCCTGTA CGTTCTCCAC ACGCTCTGGC ATGGCTTCAC CATGCCGCGT 
CTGGTACAGT GCGCCAGCAT AGTACCCTGC CCCAACACCG TGGATTGCTA CATCGCTCGG 
CCCACGGAGA AGAAGGTCTT TACCTACTTC ATGGTAGGCG CTTCTGCCGT CTGCATTATT 
CTCACCATCT GTGAGATCTG CTACCTCATC TTCCACAGGA TCATGCGAGG CATAAGCAAG 
GGCAAGTCCA CAAAGAGCAT CAGCTCCCCG AAGTCCTCCA GCCGGGCCTC CACCTGTCGC 
TGTCACCACA AGCTGCTGGA GAGTGGCGAT CCGGAAGCAG ACCCAGCCAG TGAAAAGCTG 
CAGGCTTCAG CGCCCAGCCT GACCCCCATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gjb3 ( NM_008126.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032152.1 Mus musculus gap junction protein, beta 3 (Gjb3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01392
Clone ID Related Accession (Same CDS sequence)	NM_001160012.1 , NM_008126.2
Accession Version	NM_008126.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	gap junction beta-3 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X63099.1 and AK083716.1. On May 8, 2009 this sequence version replaced NM_008126.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X63099.1, CF901204.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

ATGGACTGGA AGAAGCTCCA AGACCTATTG AGTGGCGTGA ACCAGTACTC CACGGCATTT 
GGGCGCATCT GGCTGTCAGT AGTGTTCGTC TTCCGGGTGC TGGTGTATGT GGTGGCTGCC 
GAGCGTGTGT GGGGTGACGA GCAAAAAGAC TTTGACTGTA ACACCAGGCA ACCCGGCTGT 
ACCAACGTGT GCTATGACAA CTTCTTCCCC ATCTCCAACA TCCGACTCTG GGCCCTGCAG 
CTCATCTTCG TCACGTGTCC GTCTATGCTG GTCATCCTGC ATGTAGCCTA CCGCGAGGAG 
CGGGAACGGA AGCATCGCCA GAAGCACGGG GAGCAATGCG CCAAACTGTA CAGCCACCCG 
GGCAAGAAGC ATGGCGGCCT GTGGTGGACC TACTTGTTTA GCCTCATCTT CAAGCTCATC 
ATCGAATTGG TCTTCCTGTA CGTTCTCCAC ACGCTCTGGC ATGGCTTCAC CATGCCGCGT 
CTGGTACAGT GCGCCAGCAT AGTACCCTGC CCCAACACCG TGGATTGCTA CATCGCTCGG 
CCCACGGAGA AGAAGGTCTT TACCTACTTC ATGGTAGGCG CTTCTGCCGT CTGCATTATT 
CTCACCATCT GTGAGATCTG CTACCTCATC TTCCACAGGA TCATGCGAGG CATAAGCAAG 
GGCAAGTCCA CAAAGAGCAT CAGCTCCCCG AAGTCCTCCA GCCGGGCCTC CACCTGTCGC 
TGTCACCACA AGCTGCTGGA GAGTGGCGAT CCGGAAGCAG ACCCAGCCAG TGAAAAGCTG 
CAGGCTTCAG CGCCCAGCCT GACCCCCATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032152.1
CDS	85..897
Translation	MDWKKLQDLLSGVNQYSTAFGRIWLSVVFVFRVLVYVVAAERVWGDEQKDFDCNTRQPGCTNVCYDNFFPISNIRLWALQLIFVTCPSMLVILHVAYREERERKHRQKHGEQCAKLYSHPGKKHGGLWWTYLFSLIFKLIIELVFLYVLHTLWHGFTMPRLVQCASIVPCPNTVDCYIARPTEKKVFTYFMVGASAVCIILTICEICYLIFHRIMRGISKGKSTKSISSPKSSSRASTCRCHHKLLESGDPEADPASEKLQASAPSLTPI

Target ORF information:

RefSeq Version	NM_008126.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, beta 3 (Gjb3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008126.2

ORF Insert Sequence:

ATGGACTGGA AGAAGCTCCA AGACCTATTG AGTGGCGTGA ACCAGTACTC CACGGCATTT 
GGGCGCATCT GGCTGTCAGT AGTGTTCGTC TTCCGGGTGC TGGTGTATGT GGTGGCTGCC 
GAGCGTGTGT GGGGTGACGA GCAAAAAGAC TTTGACTGTA ACACCAGGCA ACCCGGCTGT 
ACCAACGTGT GCTATGACAA CTTCTTCCCC ATCTCCAACA TCCGACTCTG GGCCCTGCAG 
CTCATCTTCG TCACGTGTCC GTCTATGCTG GTCATCCTGC ATGTAGCCTA CCGCGAGGAG 
CGGGAACGGA AGCATCGCCA GAAGCACGGG GAGCAATGCG CCAAACTGTA CAGCCACCCG 
GGCAAGAAGC ATGGCGGCCT GTGGTGGACC TACTTGTTTA GCCTCATCTT CAAGCTCATC 
ATCGAATTGG TCTTCCTGTA CGTTCTCCAC ACGCTCTGGC ATGGCTTCAC CATGCCGCGT 
CTGGTACAGT GCGCCAGCAT AGTACCCTGC CCCAACACCG TGGATTGCTA CATCGCTCGG 
CCCACGGAGA AGAAGGTCTT TACCTACTTC ATGGTAGGCG CTTCTGCCGT CTGCATTATT 
CTCACCATCT GTGAGATCTG CTACCTCATC TTCCACAGGA TCATGCGAGG CATAAGCAAG 
GGCAAGTCCA CAAAGAGCAT CAGCTCCCCG AAGTCCTCCA GCCGGGCCTC CACCTGTCGC 
TGTCACCACA AGCTGCTGGA GAGTGGCGAT CCGGAAGCAG ACCCAGCCAG TGAAAAGCTG 
CAGGCTTCAG CGCCCAGCCT GACCCCCATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increased dosage of the imprinted Ascl2 gene restrains two key endocrine lineages of the mouse Placenta.
Developmental biology418(1)55-65(2016 10)
Tunster SJ,McNamara GI,Creeth HDJ,John RM

Tpbpa-Cre-mediated deletion of TFAP2C leads to deregulation of Cdkn1a, Akt1 and the ERK pathway, causing placental growth arrest.
Development (Cambridge, England)143(5)787-98(2016 Mar)
Sharma N,Kubaczka C,Kaiser S,Nettersheim D,Mughal SS,Riesenberg S,H?lzel M,Winterhager E,Schorle H

The imprinted Phlda2 gene modulates a major endocrine compartment of the placenta to regulate placental demands for maternal resources.
Developmental biology409(1)251-60(2016 Jan)
Tunster SJ,Creeth HD,John RM

Segment-specific expression of the gap junction gene connexin31 during hindbrain development.
Development genes and evolution207(5)359-361(1997 Nov)
Dahl E,Willecke K,Balling R