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Gli2 cDNA ORF clone, Mus musculus (house mouse)

Gene Symbol Gli2
Entrez Gene ID 14633
Full Name GLI-Kruppel family member GLI2
General protein information
Preferred Names
zinc finger protein GLI2
Names
zinc finger protein GLI2
Tax helper protein
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

1

1 E2-E4|1 52.17 cM

Summary lac of sum

mRNA and Protein(s)

mRNA Protein Name
NM_001081125 NP_001074594 zinc finger protein GLI2
XM_011247924 XP_011246226 zinc finger protein GLI2 isoform X1
XM_006529141 XP_006529204 zinc finger protein GLI2 isoform X1



Homo sapiens (human) GLI2 NP_005261.2
Pan troglodytes (chimpanzee) GLI2 XP_001158280.2
Macaca mulatta (Rhesus monkey) LOC705877 XP_002808099.1
Canis lupus familiaris (dog) GLI2 XP_003432520.1
Bos taurus (cattle) GLI2 NP_001179179.2
Mus musculus (house mouse) Gli2 NP_001074594.1
Rattus norvegicus (Norway rat) Gli2 NP_001100639.1
Gallus gallus (chicken) GLI2 NP_001258830.1
Danio rerio (zebrafish) gli2a NP_571042.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100498653 XP_002943329.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

The following Gli2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gli2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OMu22271 NM_001081125 Mus musculus GLI-Kruppel family member GLI2 (Gli2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $909.30
$1299.00
OMu27904 XM_011247924 PREDICTED: Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OMu27904 XM_006529141 PREDICTED: Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu22271
Clone ID Related Accession (Same CDS sequence) NM_001081125
Accession Version NM_001081125.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 25-AUG-2015
Organism Mus musculus (house mouse)
Product zinc finger protein GLI2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ102399.1, BC085190.1, AM262744.1 and AK051858.1. On Feb 6, 2007 this sequence version replaced gi:94363912. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC082604.1, X99104.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
ATGGAGACTT CTGCCCCAGC CCCTGCACTG GAGAAGAAAG AAGCCAAGAG TGGTCTCTTG 
GAGGACAGCA GCTTCCCCGA CCCAGGGAAA AAGGCCTGTC CTCTGGCGGT GGCCGCAGCT
GTAGCCGCCC ACGGAGTTCC TCAGCAGCTC CTGCCGGCTT TCCACGCGCC TTTGCCGATT
GACATGAGAC ACCAGGAGGG AAGGTACCAT TATGACCCTC ACTCTGTCCA CAGTGTACAC
GGGCCTCCCA CCCTAAGTGG CAGCCCTGTC ATCTCAGATA TCTCCTTGAT ACGACTTTCT
CCACACCCTG CTGGCCCTGG AGAGTCACCC TTCAGCGCCC ACCACCCCTA CGTGAACCCC
CATATGGAGC ACTACCTCCG GTCTGTGCAC AGCAGCCCCA CACTCTCAAT GATCTCTGCC
GCCAGGGGCC TCAGCCCTGC TGATGTGGCC CACGAACATC TGAAAGAGAG GGGACTCTTT
AGCCTCGCAG CCCCAGGCAC CAACCCTTCA GACTATTACC ACCAGATGAC CCTCATGGCA
AGCCACCCCA CCCCTTATGG GGACCTTCTA ATGCAGAGCG GGGGTGCTGC TAGCGCACCC
CATCTCCATG ACTACCTCAA CCCTGTGGAT GCATCACGAT TCTCTAGTCC ACGTGTGACC
CCACGACTGA GCCGCAAGCG GGCTCTGTCC ATCTCCCCGC TCTCAGATGC CAGCCTCGAC
CTACAACGCA TGATTCGGAC CTCTCCCAAC TCGCTGGTAG CTTACATCAA CAACTCCAGG
AGCAGCTCAG CAGCCAGTGG CTCTTATGGA CATCTGTCTG CTGGTGCCCT CAGCCCAGCC
TTCACTTTTC CCCACCCCAT CAATCCTGTG GCCTACCAGC AGATCCTGAG CCAGCAGCGG
GGCCTGGGCT CAGCCTTTGG ACACACACCA CCCCTGATCC AGCCTTCACC CACCTTCTTG
GCCCAGCAGC CCATGACTCT CACCTCCATC AGCACCATGC CTACCCAACT CAGCAGCAGT
AGCAGCAACT GTCTAAATGA TGCCAACCAG AACAAGCAGA ACAGCGAGTC AGCTGTGAGC
AGCACCGTGA ACCCCATCAC CATTCATAAG CGGAGCAAGG TCAAGACTGA GGCTGAGGGC
CTGCGTCCAG CATCCCCGCT TGGACTGACA CAGGAGCAGC TGGCCGATCT CAAGGAAGAC
CTGGACAGGG ATGACTGTAA GCAGGAGGCC GAGGTGGTCA TCTACGAGAC CAACTGCCAC
TGGGCAGACT GCACCAAGGA GTATGACACA CAGGAGCAGC TGGTGCATCA TATCAACAAT
GAACACATCC ACGGGGAGAA GAAGGAGTTC GTGTGCCGCT GGCAGGCCTG CACGAGAGAG
CAGAAGCCCT TCAAGGCCCA GTACATGCTG GTTGTTCACA TGCGCAGACA CACGGGTGAG
AAGCCACACA AGTGCACGTT CGAAGGCTGT TCCAAGGCCT ACTCTCGCCT GGAGAACCTG
AAGACACACC TGCGTTCACA CACAGGAGAG AAGCCATATG TGTGTGAACA CGAAGGCTGT
AACAAAGCCT TCTCCAATGC CTCAGACCGC GCCAAGCACC AGAACCGCAC TCACTCCAAT
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GGTACACAGA CCCCAGCTCA
CTCCGCAAGC ATGTGAAGAC TGTCCATGGG CCAGACGCCC ATGTCACCAA GAAACAGCGT
AATGATGTGC ATGTCCGTGC TCCACTGCTC AAGGAGAATG GGGATAATGA GGCCAGCGCC
GAGCCAGGTG GCCGGGGACC TGAGGAGAGT GTGGAGGCCA GTAGCACCAG CCACACTGTG
GAGGACTGCC TACATATCAA AGCCATCAAG ACAGAGAGCT CCGGGCTTTG TCAGTCCAGC
CCCGGGGCCC AGTCATCCTG CAGCAGCGAG CCCTCTCCCC TGGGCAGTGC CCCCAACAAT
GACAGTGGCA TGGAGATGCC GGGGACAGGG CCTGGGAGTC TGGGAGACCT GACAGCACTG
GCTGACACGT GTCCAGGAGC TGACACCTCA GCCCTGGCTG CACCCTCCAC TGGTGGCCTG
CAGCTGCGCA AACACATGAG CACCGTGCAT CGCTTTGAGC AGCTGAAGAG AGAGAAGCTC
AAGTCACTGA AGGATTCCTG CTCGTGGGCC GGCCCAGCTC CACACACCCG CAACACCAAG
CTGCCTCCCC TTCCAGTCAA TGGTTCTGTC CTGGAAAACT TCAACAATAC AGGGGGCGGT
GGACCGGCAG GACTGCTGCC CAGCCAGCGG CTACCAGAGC TGACCGAAGT GACGATGCTG
AGCCAGCTGC AGGAACGAAG AGACAGCTCC ACCAGCACCA TGAGCTCGGC CTACACTGTG
AGCCGCCGCT CCTCTGGCAT CTCCCCATAC TTCTCTAGCC GTCGCTCCAG CGAGGCTTCG
CCTCTCGGTG GCCTACGCCC GCACAACGCC AGCTCAGCAG ACTCCTATGA CCCCATCTCC
ACAGATGCCT CTCGGCGCTC CAGTGAAGCC AGCCAGTGCA GTGGCGGTGG CCCAGGGCTG
CTCAACCTCA CACCTGCGCA GCAGTACAAC CTGCGTGCCA AGTACGCAGC GGCCACAGGT
GGACCACCGC CCACGCCACT GCCGGGCCTC GATCGTGTAA GCCTTCGTAC CCGCCTGGCC
TTGCTGGATG CTCCTGAGCG TGCACTTCCT GGTGCCTGCC CACATCCACT GGGGCCACGG
CGTGGCAGCG ATGGGCCTAC CTATAGCCAT GGTCATGGCC ATGGCTACGC AGGTGCGGCT
CCAGCATTCC CCCACGAGGG GCCAAACAGC AGCACACGGC GGGCCAGCGA CCCTGTGCGG
CGCCCTGACC CCCTTATTCT GCCTCGAGTG CAACGTTTCC ACAGTACCCA CAACATGAAT
CCAGGTTCAC TGCCACCCTG CGCTGATCGG CGTGGCCTGC ACGTACAGAG CCACCCCAGC
GTAGACAGCA ACCTGACCCG CAACGCCTAC TCTCCCAGAC CCCCTAGCAT CAATGAGAAC
GTGGTGATGG AGGCCGTGGC TGCTGGGGTA GACGGCCCAG GGCTAGAGTG CGACCTGGGG
CTGGTGGAGG ATGAGCTGGT GCTGCCAGAT GATGTGGTAC AGTACATCAA GGCTCACACC
GGTGGTACCT TGGATGACGG CATTCGGCAG GGGTATCCCA CAGAAGGTAC TGGCTTCCCC
GAGAACTCTA AGCTGCCCAG TCCTGGGCTA CAAGGCCACC GCAGGCTAGC AGCTGCCGAC
TCCAACATGG GTCCTTCTGC TCCTGGACTC GGGGGCTGCC AGCTGAGCTA CAGCCCCTCC
TCCAACCTCA ACAAGAGCAA CATGCCTGTG CAGTGGAATG AGGTGAGTTC TGGCACCGTG
GATGCCCTGC CTACCCAGGT GAAGCCACCT CCTTTCCCTC ACAGCAACCT GGCTGTGGTC
CAACAGAAGC CAGCCTTTGG CCAGTATCCA GGATATAATC CACAATCCGT GCAGAGCAGC
TCCGGAGGTC TAGACAGCAC CCAGCCGCAC CTACAGCTTC GAGGAGCCCC CTCTGCATCA
AGAGGGAGCT ACACGCAACA GCCTCGACAG CCAGCTGCAG GCAGTCAGTG CCTGGGTATG
AGTGCGGCCA TGAGCCCGCA GGCCAGCTAC AGCCAAGCCC ACCCCCAGCT GAGCCCAAAC
ATTGTCAGCG GATCTCTGAA CCAGTTTTCT CCCTCCTGCA GCAATATGGC AGCCAAGCCC
AGCCACCTGG GACTCCCTCA GCAAATGGAA GTTGTCCCCA ATGCCACCAT CATGAATGGC
CATCAACGGG AGCACGGGGT CCCCAATTCA TCCCTGGCTG CGGTGTCACA ACCTCACCCA
GTCCTGAGCT ATCCCCAGCA GGACAGCTAC CAACAGGGCT CCAACCTTCT GTCATCCCAT
CAGCCTGGCT TCATGGAGTC CCAGCAGAAC GCGGGCTTTG GTCTCATGCA GCCTCGGCCA
CCCCTGGAAC CCAACACGGC CAGCCGTCAC CGTGGAGTAC GTTCTGGGCA ACAGCAGTTG
TATGCCAGGA CCACTGGCCA AGCCATGGTC ACATCAGCCA ACCAAGAGAC AGCAGAAGCT
ATGCCCAAGG GACCAGCAGG GACCATGGTA TCCCTAGCTC CTCAGCCATC TCAGGACACA
GGGCGGGCAC AAGATCAGAA CACGCTATAC TACTATGGCC AGATCCACAT GTATGAACAG
AATGGAGGCT GCCCAGCCGT GCAGCCCCAG CCGCCACAAC CACAAGCTTG CTCAGACAGT
ATCCAGCCTG AGCCTTTGCC TTCACCGGGA GTCAACCAGG TGTCTAGCAC CGTGGACTCC
CAGCTCCTGG AGCCCCCCCA GATTGACTTT GATGCCATCA TGGATGATGG TGATCACTCG
AGTTTGTTTT CTGGTGCACT GAGCCCAACC CTTCTCCACA ATCTCTCCCA GAATTCCTCA
CGCCTCACCA CACCCCGGAA TTCCTTGACA CTGCCCTCCA TCCCTGCGGG CATCAGCAAC
ATGGCCGTGG GCGACATGAG TTCCATGCTC ACCAGCCTGG CTGAAGAGAG CAAGTTTTTA
AACATGATGA CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001074594.1
CDS350..4984
Misc Feature(1)338..340(+)
Misc Feature(2)782..784(+)
Misc Feature(3)1037..1039(+)
Misc Feature(4)1043..1045(+)
Misc Feature(5)1061..1063(+)
Misc Feature(6)1502..1504(+)
Misc Feature(7)1733..1960(+)
Misc Feature(8)1733..1780(+)
Misc Feature(9)1754..1837(+)
Misc Feature(10)1766..1780(+)
Misc Feature(11)1802..1870(+)
Misc Feature(12)1802..1867(+)
Misc Feature(13)1844..1927(+)
Misc Feature(14)1892..1963(+)
Misc Feature(15)1892..1963(+)
Misc Feature(16)1901..1963(+)
Misc Feature(17)1907..1963(+)
Misc Feature(18)1913..2050(+)
Misc Feature(19)1985..2050(+)
Misc Feature(20)1985..2041(+)
Misc Feature(21)2468..2470(+)
Misc Feature(22)2471..2473(+)
Misc Feature(23)2567..2569(+)
Misc Feature(24)3338..3340(+)
Exon (1)1..318
Gene:Gli2
Gene Synonym:
Exon (2)319..485
Gene:Gli2
Gene Synonym:
Exon (3)486..591
Gene:Gli2
Gene Synonym:
Exon (4)592..794
Gene:Gli2
Gene Synonym:
Exon (5)795..980
Gene:Gli2
Gene Synonym:
Exon (6)981..1182
Gene:Gli2
Gene Synonym:
Exon (7)1183..1399
Gene:Gli2
Gene Synonym:
Exon (8)1400..1522
Gene:Gli2
Gene Synonym:
Exon (9)1523..1657
Gene:Gli2
Gene Synonym:
Exon (10)1658..1807
Gene:Gli2
Gene Synonym:
Exon (11)1808..1972
Gene:Gli2
Gene Synonym:
Exon (12)1973..2254
Gene:Gli2
Gene Synonym:
Exon (13)2255..2591
Gene:Gli2
Gene Synonym:
Exon (14)2592..6708
Gene:Gli2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001081125
Organism Mus musculus (house mouse)
Definition Mus musculus GLI-Kruppel family member GLI2 (Gli2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001081125

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
ATGGAGACTT CTGCCCCAGC CCCTGCACTG GAGAAGAAAG AAGCCAAGAG TGGTCTCTTG 
GAGGACAGCA GCTTCCCCGA CCCAGGGAAA AAGGCCTGTC CTCTGGCGGT GGCCGCAGCT
GTAGCCGCCC ACGGAGTTCC TCAGCAGCTC CTGCCGGCTT TCCACGCGCC TTTGCCGATT
GACATGAGAC ACCAGGAGGG AAGGTACCAT TATGACCCTC ACTCTGTCCA CAGTGTACAC
GGGCCTCCCA CCCTAAGTGG CAGCCCTGTC ATCTCAGATA TCTCCTTGAT ACGACTTTCT
CCACACCCTG CTGGCCCTGG AGAGTCACCC TTCAGCGCCC ACCACCCCTA CGTGAACCCC
CATATGGAGC ACTACCTCCG GTCTGTGCAC AGCAGCCCCA CACTCTCAAT GATCTCTGCC
GCCAGGGGCC TCAGCCCTGC TGATGTGGCC CACGAACATC TGAAAGAGAG GGGACTCTTT
AGCCTCGCAG CCCCAGGCAC CAACCCTTCA GACTATTACC ACCAGATGAC CCTCATGGCA
AGCCACCCCA CCCCTTATGG GGACCTTCTA ATGCAGAGCG GGGGTGCTGC TAGCGCACCC
CATCTCCATG ACTACCTCAA CCCTGTGGAT GCATCACGAT TCTCTAGTCC ACGTGTGACC
CCACGACTGA GCCGCAAGCG GGCTCTGTCC ATCTCCCCGC TCTCAGATGC CAGCCTCGAC
CTACAACGCA TGATTCGGAC CTCTCCCAAC TCGCTGGTAG CTTACATCAA CAACTCCAGG
AGCAGCTCAG CAGCCAGTGG CTCTTATGGA CATCTGTCTG CTGGTGCCCT CAGCCCAGCC
TTCACTTTTC CCCACCCCAT CAATCCTGTG GCCTACCAGC AGATCCTGAG CCAGCAGCGG
GGCCTGGGCT CAGCCTTTGG ACACACACCA CCCCTGATCC AGCCTTCACC CACCTTCTTG
GCCCAGCAGC CCATGACTCT CACCTCCATC AGCACCATGC CTACCCAACT CAGCAGCAGT
AGCAGCAACT GTCTAAATGA TGCCAACCAG AACAAGCAGA ACAGCGAGTC AGCTGTGAGC
AGCACCGTGA ACCCCATCAC CATTCATAAG CGGAGCAAGG TCAAGACTGA GGCTGAGGGC
CTGCGTCCAG CATCCCCGCT TGGACTGACA CAGGAGCAGC TGGCCGATCT CAAGGAAGAC
CTGGACAGGG ATGACTGTAA GCAGGAGGCC GAGGTGGTCA TCTACGAGAC CAACTGCCAC
TGGGCAGACT GCACCAAGGA GTATGACACA CAGGAGCAGC TGGTGCATCA TATCAACAAT
GAACACATCC ACGGGGAGAA GAAGGAGTTC GTGTGCCGCT GGCAGGCCTG CACGAGAGAG
CAGAAGCCCT TCAAGGCCCA GTACATGCTG GTTGTTCACA TGCGCAGACA CACGGGTGAG
AAGCCACACA AGTGCACGTT CGAAGGCTGT TCCAAGGCCT ACTCTCGCCT GGAGAACCTG
AAGACACACC TGCGTTCACA CACAGGAGAG AAGCCATATG TGTGTGAACA CGAAGGCTGT
AACAAAGCCT TCTCCAATGC CTCAGACCGC GCCAAGCACC AGAACCGCAC TCACTCCAAT
GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GGTACACAGA CCCCAGCTCA
CTCCGCAAGC ATGTGAAGAC TGTCCATGGG CCAGACGCCC ATGTCACCAA GAAACAGCGT
AATGATGTGC ATGTCCGTGC TCCACTGCTC AAGGAGAATG GGGATAATGA GGCCAGCGCC
GAGCCAGGTG GCCGGGGACC TGAGGAGAGT GTGGAGGCCA GTAGCACCAG CCACACTGTG
GAGGACTGCC TACATATCAA AGCCATCAAG ACAGAGAGCT CCGGGCTTTG TCAGTCCAGC
CCCGGGGCCC AGTCATCCTG CAGCAGCGAG CCCTCTCCCC TGGGCAGTGC CCCCAACAAT
GACAGTGGCA TGGAGATGCC GGGGACAGGG CCTGGGAGTC TGGGAGACCT GACAGCACTG
GCTGACACGT GTCCAGGAGC TGACACCTCA GCCCTGGCTG CACCCTCCAC TGGTGGCCTG
CAGCTGCGCA AACACATGAG CACCGTGCAT CGCTTTGAGC AGCTGAAGAG AGAGAAGCTC
AAGTCACTGA AGGATTCCTG CTCGTGGGCC GGCCCAGCTC CACACACCCG CAACACCAAG
CTGCCTCCCC TTCCAGTCAA TGGTTCTGTC CTGGAAAACT TCAACAATAC AGGGGGCGGT
GGACCGGCAG GACTGCTGCC CAGCCAGCGG CTACCAGAGC TGACCGAAGT GACGATGCTG
AGCCAGCTGC AGGAACGAAG AGACAGCTCC ACCAGCACCA TGAGCTCGGC CTACACTGTG
AGCCGCCGCT CCTCTGGCAT CTCCCCATAC TTCTCTAGCC GTCGCTCCAG CGAGGCTTCG
CCTCTCGGTG GCCTACGCCC GCACAACGCC AGCTCAGCAG ACTCCTATGA CCCCATCTCC
ACAGATGCCT CTCGGCGCTC CAGTGAAGCC AGCCAGTGCA GTGGCGGTGG CCCAGGGCTG
CTCAACCTCA CACCTGCGCA GCAGTACAAC CTGCGTGCCA AGTACGCAGC GGCCACAGGT
GGACCACCGC CCACGCCACT GCCGGGCCTC GATCGTGTAA GCCTTCGTAC CCGCCTGGCC
TTGCTGGATG CTCCTGAGCG TGCACTTCCT GGTGCCTGCC CACATCCACT GGGGCCACGG
CGTGGCAGCG ATGGGCCTAC CTATAGCCAT GGTCATGGCC ATGGCTACGC AGGTGCGGCT
CCAGCATTCC CCCACGAGGG GCCAAACAGC AGCACACGGC GGGCCAGCGA CCCTGTGCGG
CGCCCTGACC CCCTTATTCT GCCTCGAGTG CAACGTTTCC ACAGTACCCA CAACATGAAT
CCAGGTTCAC TGCCACCCTG CGCTGATCGG CGTGGCCTGC ACGTACAGAG CCACCCCAGC
GTAGACAGCA ACCTGACCCG CAACGCCTAC TCTCCCAGAC CCCCTAGCAT CAATGAGAAC
GTGGTGATGG AGGCCGTGGC TGCTGGGGTA GACGGCCCAG GGCTAGAGTG CGACCTGGGG
CTGGTGGAGG ATGAGCTGGT GCTGCCAGAT GATGTGGTAC AGTACATCAA GGCTCACACC
GGTGGTACCT TGGATGACGG CATTCGGCAG GGGTATCCCA CAGAAGGTAC TGGCTTCCCC
GAGAACTCTA AGCTGCCCAG TCCTGGGCTA CAAGGCCACC GCAGGCTAGC AGCTGCCGAC
TCCAACATGG GTCCTTCTGC TCCTGGACTC GGGGGCTGCC AGCTGAGCTA CAGCCCCTCC
TCCAACCTCA ACAAGAGCAA CATGCCTGTG CAGTGGAATG AGGTGAGTTC TGGCACCGTG
GATGCCCTGC CTACCCAGGT GAAGCCACCT CCTTTCCCTC ACAGCAACCT GGCTGTGGTC
CAACAGAAGC CAGCCTTTGG CCAGTATCCA GGATATAATC CACAATCCGT GCAGAGCAGC
TCCGGAGGTC TAGACAGCAC CCAGCCGCAC CTACAGCTTC GAGGAGCCCC CTCTGCATCA
AGAGGGAGCT ACACGCAACA GCCTCGACAG CCAGCTGCAG GCAGTCAGTG CCTGGGTATG
AGTGCGGCCA TGAGCCCGCA GGCCAGCTAC AGCCAAGCCC ACCCCCAGCT GAGCCCAAAC
ATTGTCAGCG GATCTCTGAA CCAGTTTTCT CCCTCCTGCA GCAATATGGC AGCCAAGCCC
AGCCACCTGG GACTCCCTCA GCAAATGGAA GTTGTCCCCA ATGCCACCAT CATGAATGGC
CATCAACGGG AGCACGGGGT CCCCAATTCA TCCCTGGCTG CGGTGTCACA ACCTCACCCA
GTCCTGAGCT ATCCCCAGCA GGACAGCTAC CAACAGGGCT CCAACCTTCT GTCATCCCAT
CAGCCTGGCT TCATGGAGTC CCAGCAGAAC GCGGGCTTTG GTCTCATGCA GCCTCGGCCA
CCCCTGGAAC CCAACACGGC CAGCCGTCAC CGTGGAGTAC GTTCTGGGCA ACAGCAGTTG
TATGCCAGGA CCACTGGCCA AGCCATGGTC ACATCAGCCA ACCAAGAGAC AGCAGAAGCT
ATGCCCAAGG GACCAGCAGG GACCATGGTA TCCCTAGCTC CTCAGCCATC TCAGGACACA
GGGCGGGCAC AAGATCAGAA CACGCTATAC TACTATGGCC AGATCCACAT GTATGAACAG
AATGGAGGCT GCCCAGCCGT GCAGCCCCAG CCGCCACAAC CACAAGCTTG CTCAGACAGT
ATCCAGCCTG AGCCTTTGCC TTCACCGGGA GTCAACCAGG TGTCTAGCAC CGTGGACTCC
CAGCTCCTGG AGCCCCCCCA GATTGACTTT GATGCCATCA TGGATGATGG TGATCACTCG
AGTTTGTTTT CTGGTGCACT GAGCCCAACC CTTCTCCACA ATCTCTCCCA GAATTCCTCA
CGCCTCACCA CACCCCGGAA TTCCTTGACA CTGCCCTCCA TCCCTGCGGG CATCAGCAAC
ATGGCCGTGG GCGACATGAG TTCCATGCTC ACCAGCCTGG CTGAAGAGAG CAAGTTTTTA
AACATGATGA CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu27904
Clone ID Related Accession (Same CDS sequence) XM_006529141 , XM_011247924
Accession Version XM_011247924.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product zinc finger protein GLI2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_078297.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACT CTGTCCACAG TGTACACGGG 
CCTCCCACCC TAAGTGGCAG CCCTGTCATC TCAGATATCT CCTTGATACG ACTTTCTCCA
CACCCTGCTG GCCCTGGAGA GTCACCCTTC AGCGCCCACC ACCCCTACGT GAACCCCCAT
ATGGAGCACT ACCTCCGGTC TGTGCACAGC AGCCCCACAC TCTCAATGAT CTCTGCCGCC
AGGGGCCTCA GCCCTGCTGA TGTGGCCCAC GAACATCTGA AAGAGAGGGG ACTCTTTAGC
CTCGCAGCCC CAGGCACCAA CCCTTCAGAC TATTACCACC AGATGACCCT CATGGCAAGC
CACCCCACCC CTTATGGGGA CCTTCTAATG CAGAGCGGGG GTGCTGCTAG CGCACCCCAT
CTCCATGACT ACCTCAACCC TGTGGATGCA TCACGATTCT CTAGTCCACG TGTGACCCCA
CGACTGAGCC GCAAGCGGGC TCTGTCCATC TCCCCGCTCT CAGATGCCAG CCTCGACCTA
CAACGCATGA TTCGGACCTC TCCCAACTCG CTGGTAGCTT ACATCAACAA CTCCAGGAGC
AGCTCAGCAG CCAGTGGCTC TTATGGACAT CTGTCTGCTG GTGCCCTCAG CCCAGCCTTC
ACTTTTCCCC ACCCCATCAA TCCTGTGGCC TACCAGCAGA TCCTGAGCCA GCAGCGGGGC
CTGGGCTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CTTCACCCAC CTTCTTGGCC
CAGCAGCCCA TGACTCTCAC CTCCATCAGC ACCATGCCTA CCCAACTCAG CAGCAGTAGC
AGCAACTGTC TAAATGATGC CAACCAGAAC AAGCAGAACA GCGAGTCAGC TGTGAGCAGC
ACCGTGAACC CCATCACCAT TCATAAGCGG AGCAAGGTCA AGACTGAGGC TGAGGGCCTG
CGTCCAGCAT CCCCGCTTGG ACTGACACAG GAGCAGCTGG CCGATCTCAA GGAAGACCTG
GACAGGGATG ACTGTAAGCA GGAGGCCGAG GTGGTCATCT ACGAGACCAA CTGCCACTGG
GCAGACTGCA CCAAGGAGTA TGACACACAG GAGCAGCTGG TGCATCATAT CAACAATGAA
CACATCCACG GGGAGAAGAA GGAGTTCGTG TGCCGCTGGC AGGCCTGCAC GAGAGAGCAG
AAGCCCTTCA AGGCCCAGTA CATGCTGGTT GTTCACATGC GCAGACACAC GGGTGAGAAG
CCACACAAGT GCACGTTCGA AGGCTGTTCC AAGGCCTACT CTCGCCTGGA GAACCTGAAG
ACACACCTGC GTTCACACAC AGGAGAGAAG CCATATGTGT GTGAACACGA AGGCTGTAAC
AAAGCCTTCT CCAATGCCTC AGACCGCGCC AAGCACCAGA ACCGCACTCA CTCCAATGAG
AAACCCTACA TCTGCAAGAT CCCAGGCTGC ACCAAGAGGT ACACAGACCC CAGCTCACTC
CGCAAGCATG TGAAGACTGT CCATGGGCCA GACGCCCATG TCACCAAGAA ACAGCGTAAT
GATGTGCATG TCCGTGCTCC ACTGCTCAAG GAGAATGGGG ATAATGAGGC CAGCGCCGAG
CCAGGTGGCC GGGGACCTGA GGAGAGTGTG GAGGCCAGTA GCACCAGCCA CACTGTGGAG
GACTGCCTAC ATATCAAAGC CATCAAGACA GAGAGCTCCG GGCTTTGTCA GTCCAGCCCC
GGGGCCCAGT CATCCTGCAG CAGCGAGCCC TCTCCCCTGG GCAGTGCCCC CAACAATGAC
AGTGGCATGG AGATGCCGGG GACAGGGCCT GGGAGTCTGG GAGACCTGAC AGCACTGGCT
GACACGTGTC CAGGAGCTGA CACCTCAGCC CTGGCTGCAC CCTCCACTGG TGGCCTGCAG
CTGCGCAAAC ACATGAGCAC CGTGCATCGC TTTGAGCAGC TGAAGAGAGA GAAGCTCAAG
TCACTGAAGG ATTCCTGCTC GTGGGCCGGC CCAGCTCCAC ACACCCGCAA CACCAAGCTG
CCTCCCCTTC CAGTCAATGG TTCTGTCCTG GAAAACTTCA ACAATACAGG GGGCGGTGGA
CCGGCAGGAC TGCTGCCCAG CCAGCGGCTA CCAGAGCTGA CCGAAGTGAC GATGCTGAGC
CAGCTGCAGG AACGAAGAGA CAGCTCCACC AGCACCATGA GCTCGGCCTA CACTGTGAGC
CGCCGCTCCT CTGGCATCTC CCCATACTTC TCTAGCCGTC GCTCCAGCGA GGCTTCGCCT
CTCGGTGGCC TACGCCCGCA CAACGCCAGC TCAGCAGACT CCTATGACCC CATCTCCACA
GATGCCTCTC GGCGCTCCAG TGAAGCCAGC CAGTGCAGTG GCGGTGGCCC AGGGCTGCTC
AACCTCACAC CTGCGCAGCA GTACAACCTG CGTGCCAAGT ACGCAGCGGC CACAGGTGGA
CCACCGCCCA CGCCACTGCC GGGCCTCGAT CGTGTAAGCC TTCGTACCCG CCTGGCCTTG
CTGGATGCTC CTGAGCGTGC ACTTCCTGGT GCCTGCCCAC ATCCACTGGG GCCACGGCGT
GGCAGCGATG GGCCTACCTA TAGCCATGGT CATGGCCATG GCTACGCAGG TGCGGCTCCA
GCATTCCCCC ACGAGGGGCC AAACAGCAGC ACACGGCGGG CCAGCGACCC TGTGCGGCGC
CCTGACCCCC TTATTCTGCC TCGAGTGCAA CGTTTCCACA GTACCCACAA CATGAATCCA
GGTTCACTGC CACCCTGCGC TGATCGGCGT GGCCTGCACG TACAGAGCCA CCCCAGCGTA
GACAGCAACC TGACCCGCAA CGCCTACTCT CCCAGACCCC CTAGCATCAA TGAGAACGTG
GTGATGGAGG CCGTGGCTGC TGGGGTAGAC GGCCCAGGGC TAGAGTGCGA CCTGGGGCTG
GTGGAGGATG AGCTGGTGCT GCCAGATGAT GTGGTACAGT ACATCAAGGC TCACACCGGT
GGTACCTTGG ATGACGGCAT TCGGCAGGGG TATCCCACAG AAGGTACTGG CTTCCCCGAG
AACTCTAAGC TGCCCAGTCC TGGGCTACAA GGCCACCGCA GGCTAGCAGC TGCCGACTCC
AACATGGGTC CTTCTGCTCC TGGACTCGGG GGCTGCCAGC TGAGCTACAG CCCCTCCTCC
AACCTCAACA AGAGCAACAT GCCTGTGCAG TGGAATGAGG TGAGTTCTGG CACCGTGGAT
GCCCTGCCTA CCCAGGTGAA GCCACCTCCT TTCCCTCACA GCAACCTGGC TGTGGTCCAA
CAGAAGCCAG CCTTTGGCCA GTATCCAGGA TATAATCCAC AATCCGTGCA GAGCAGCTCC
GGAGGTCTAG ACAGCACCCA GCCGCACCTA CAGCTTCGAG GAGCCCCCTC TGCATCAAGA
GGGAGCTACA CGCAACAGCC TCGACAGCCA GCTGCAGGCA GTCAGTGCCT GGGTATGAGT
GCGGCCATGA GCCCGCAGGC CAGCTACAGC CAAGCCCACC CCCAGCTGAG CCCAAACATT
GTCAGCGGAT CTCTGAACCA GTTTTCTCCC TCCTGCAGCA ATATGGCAGC CAAGCCCAGC
CACCTGGGAC TCCCTCAGCA AATGGAAGTT GTCCCCAATG CCACCATCAT GAATGGCCAT
CAACGGGAGC ACGGGGTCCC CAATTCATCC CTGGCTGCGG TGTCACAACC TCACCCAGTC
CTGAGCTATC CCCAGCAGGA CAGCTACCAA CAGGGCTCCA ACCTTCTGTC ATCCCATCAG
CCTGGCTTCA TGGAGTCCCA GCAGAACGCG GGCTTTGGTC TCATGCAGCC TCGGCCACCC
CTGGAACCCA ACACGGCCAG CCGTCACCGT GGAGTACGTT CTGGGCAACA GCAGTTGTAT
GCCAGGACCA CTGGCCAAGC CATGGTCACA TCAGCCAACC AAGAGACAGC AGAAGCTATG
CCCAAGGGAC CAGCAGGGAC CATGGTATCC CTAGCTCCTC AGCCATCTCA GGACACAGGG
CGGGCACAAG ATCAGAACAC GCTATACTAC TATGGCCAGA TCCACATGTA TGAACAGAAT
GGAGGCTGCC CAGCCGTGCA GCCCCAGCCG CCACAACCAC AAGCTTGCTC AGACAGTATC
CAGCCTGAGC CTTTGCCTTC ACCGGGAGTC AACCAGGTGT CTAGCACCGT GGACTCCCAG
CTCCTGGAGC CCCCCCAGAT TGACTTTGAT GCCATCATGG ATGATGGTGA TCACTCGAGT
TTGTTTTCTG GTGCACTGAG CCCAACCCTT CTCCACAATC TCTCCCAGAA TTCCTCACGC
CTCACCACAC CCCGGAATTC CTTGACACTG CCCTCCATCC CTGCGGGCAT CAGCAACATG
GCCGTGGGCG ACATGAGTTC CATGCTCACC AGCCTGGCTG AAGAGAGCAA GTTTTTAAAC
ATGATGACCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011246226.1
CDS916..5367
Misc Feature(1)2116..2343(+)
Misc Feature(2)2116..2163(+)
Misc Feature(3)2137..2220(+)
Misc Feature(4)2149..2163(+)
Misc Feature(5)2185..2253(+)
Misc Feature(6)2185..2250(+)
Misc Feature(7)2227..2310(+)
Misc Feature(8)2275..2346(+)
Misc Feature(9)2275..2346(+)
Misc Feature(10)2284..2346(+)
Misc Feature(11)2290..2346(+)
Misc Feature(12)2296..2433(+)
Misc Feature(13)2368..2433(+)
Misc Feature(14)2368..2424(+)
Translation

Target ORF information:

RefSeq Version XM_011247924
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011247924

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACT CTGTCCACAG TGTACACGGG 
CCTCCCACCC TAAGTGGCAG CCCTGTCATC TCAGATATCT CCTTGATACG ACTTTCTCCA
CACCCTGCTG GCCCTGGAGA GTCACCCTTC AGCGCCCACC ACCCCTACGT GAACCCCCAT
ATGGAGCACT ACCTCCGGTC TGTGCACAGC AGCCCCACAC TCTCAATGAT CTCTGCCGCC
AGGGGCCTCA GCCCTGCTGA TGTGGCCCAC GAACATCTGA AAGAGAGGGG ACTCTTTAGC
CTCGCAGCCC CAGGCACCAA CCCTTCAGAC TATTACCACC AGATGACCCT CATGGCAAGC
CACCCCACCC CTTATGGGGA CCTTCTAATG CAGAGCGGGG GTGCTGCTAG CGCACCCCAT
CTCCATGACT ACCTCAACCC TGTGGATGCA TCACGATTCT CTAGTCCACG TGTGACCCCA
CGACTGAGCC GCAAGCGGGC TCTGTCCATC TCCCCGCTCT CAGATGCCAG CCTCGACCTA
CAACGCATGA TTCGGACCTC TCCCAACTCG CTGGTAGCTT ACATCAACAA CTCCAGGAGC
AGCTCAGCAG CCAGTGGCTC TTATGGACAT CTGTCTGCTG GTGCCCTCAG CCCAGCCTTC
ACTTTTCCCC ACCCCATCAA TCCTGTGGCC TACCAGCAGA TCCTGAGCCA GCAGCGGGGC
CTGGGCTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CTTCACCCAC CTTCTTGGCC
CAGCAGCCCA TGACTCTCAC CTCCATCAGC ACCATGCCTA CCCAACTCAG CAGCAGTAGC
AGCAACTGTC TAAATGATGC CAACCAGAAC AAGCAGAACA GCGAGTCAGC TGTGAGCAGC
ACCGTGAACC CCATCACCAT TCATAAGCGG AGCAAGGTCA AGACTGAGGC TGAGGGCCTG
CGTCCAGCAT CCCCGCTTGG ACTGACACAG GAGCAGCTGG CCGATCTCAA GGAAGACCTG
GACAGGGATG ACTGTAAGCA GGAGGCCGAG GTGGTCATCT ACGAGACCAA CTGCCACTGG
GCAGACTGCA CCAAGGAGTA TGACACACAG GAGCAGCTGG TGCATCATAT CAACAATGAA
CACATCCACG GGGAGAAGAA GGAGTTCGTG TGCCGCTGGC AGGCCTGCAC GAGAGAGCAG
AAGCCCTTCA AGGCCCAGTA CATGCTGGTT GTTCACATGC GCAGACACAC GGGTGAGAAG
CCACACAAGT GCACGTTCGA AGGCTGTTCC AAGGCCTACT CTCGCCTGGA GAACCTGAAG
ACACACCTGC GTTCACACAC AGGAGAGAAG CCATATGTGT GTGAACACGA AGGCTGTAAC
AAAGCCTTCT CCAATGCCTC AGACCGCGCC AAGCACCAGA ACCGCACTCA CTCCAATGAG
AAACCCTACA TCTGCAAGAT CCCAGGCTGC ACCAAGAGGT ACACAGACCC CAGCTCACTC
CGCAAGCATG TGAAGACTGT CCATGGGCCA GACGCCCATG TCACCAAGAA ACAGCGTAAT
GATGTGCATG TCCGTGCTCC ACTGCTCAAG GAGAATGGGG ATAATGAGGC CAGCGCCGAG
CCAGGTGGCC GGGGACCTGA GGAGAGTGTG GAGGCCAGTA GCACCAGCCA CACTGTGGAG
GACTGCCTAC ATATCAAAGC CATCAAGACA GAGAGCTCCG GGCTTTGTCA GTCCAGCCCC
GGGGCCCAGT CATCCTGCAG CAGCGAGCCC TCTCCCCTGG GCAGTGCCCC CAACAATGAC
AGTGGCATGG AGATGCCGGG GACAGGGCCT GGGAGTCTGG GAGACCTGAC AGCACTGGCT
GACACGTGTC CAGGAGCTGA CACCTCAGCC CTGGCTGCAC CCTCCACTGG TGGCCTGCAG
CTGCGCAAAC ACATGAGCAC CGTGCATCGC TTTGAGCAGC TGAAGAGAGA GAAGCTCAAG
TCACTGAAGG ATTCCTGCTC GTGGGCCGGC CCAGCTCCAC ACACCCGCAA CACCAAGCTG
CCTCCCCTTC CAGTCAATGG TTCTGTCCTG GAAAACTTCA ACAATACAGG GGGCGGTGGA
CCGGCAGGAC TGCTGCCCAG CCAGCGGCTA CCAGAGCTGA CCGAAGTGAC GATGCTGAGC
CAGCTGCAGG AACGAAGAGA CAGCTCCACC AGCACCATGA GCTCGGCCTA CACTGTGAGC
CGCCGCTCCT CTGGCATCTC CCCATACTTC TCTAGCCGTC GCTCCAGCGA GGCTTCGCCT
CTCGGTGGCC TACGCCCGCA CAACGCCAGC TCAGCAGACT CCTATGACCC CATCTCCACA
GATGCCTCTC GGCGCTCCAG TGAAGCCAGC CAGTGCAGTG GCGGTGGCCC AGGGCTGCTC
AACCTCACAC CTGCGCAGCA GTACAACCTG CGTGCCAAGT ACGCAGCGGC CACAGGTGGA
CCACCGCCCA CGCCACTGCC GGGCCTCGAT CGTGTAAGCC TTCGTACCCG CCTGGCCTTG
CTGGATGCTC CTGAGCGTGC ACTTCCTGGT GCCTGCCCAC ATCCACTGGG GCCACGGCGT
GGCAGCGATG GGCCTACCTA TAGCCATGGT CATGGCCATG GCTACGCAGG TGCGGCTCCA
GCATTCCCCC ACGAGGGGCC AAACAGCAGC ACACGGCGGG CCAGCGACCC TGTGCGGCGC
CCTGACCCCC TTATTCTGCC TCGAGTGCAA CGTTTCCACA GTACCCACAA CATGAATCCA
GGTTCACTGC CACCCTGCGC TGATCGGCGT GGCCTGCACG TACAGAGCCA CCCCAGCGTA
GACAGCAACC TGACCCGCAA CGCCTACTCT CCCAGACCCC CTAGCATCAA TGAGAACGTG
GTGATGGAGG CCGTGGCTGC TGGGGTAGAC GGCCCAGGGC TAGAGTGCGA CCTGGGGCTG
GTGGAGGATG AGCTGGTGCT GCCAGATGAT GTGGTACAGT ACATCAAGGC TCACACCGGT
GGTACCTTGG ATGACGGCAT TCGGCAGGGG TATCCCACAG AAGGTACTGG CTTCCCCGAG
AACTCTAAGC TGCCCAGTCC TGGGCTACAA GGCCACCGCA GGCTAGCAGC TGCCGACTCC
AACATGGGTC CTTCTGCTCC TGGACTCGGG GGCTGCCAGC TGAGCTACAG CCCCTCCTCC
AACCTCAACA AGAGCAACAT GCCTGTGCAG TGGAATGAGG TGAGTTCTGG CACCGTGGAT
GCCCTGCCTA CCCAGGTGAA GCCACCTCCT TTCCCTCACA GCAACCTGGC TGTGGTCCAA
CAGAAGCCAG CCTTTGGCCA GTATCCAGGA TATAATCCAC AATCCGTGCA GAGCAGCTCC
GGAGGTCTAG ACAGCACCCA GCCGCACCTA CAGCTTCGAG GAGCCCCCTC TGCATCAAGA
GGGAGCTACA CGCAACAGCC TCGACAGCCA GCTGCAGGCA GTCAGTGCCT GGGTATGAGT
GCGGCCATGA GCCCGCAGGC CAGCTACAGC CAAGCCCACC CCCAGCTGAG CCCAAACATT
GTCAGCGGAT CTCTGAACCA GTTTTCTCCC TCCTGCAGCA ATATGGCAGC CAAGCCCAGC
CACCTGGGAC TCCCTCAGCA AATGGAAGTT GTCCCCAATG CCACCATCAT GAATGGCCAT
CAACGGGAGC ACGGGGTCCC CAATTCATCC CTGGCTGCGG TGTCACAACC TCACCCAGTC
CTGAGCTATC CCCAGCAGGA CAGCTACCAA CAGGGCTCCA ACCTTCTGTC ATCCCATCAG
CCTGGCTTCA TGGAGTCCCA GCAGAACGCG GGCTTTGGTC TCATGCAGCC TCGGCCACCC
CTGGAACCCA ACACGGCCAG CCGTCACCGT GGAGTACGTT CTGGGCAACA GCAGTTGTAT
GCCAGGACCA CTGGCCAAGC CATGGTCACA TCAGCCAACC AAGAGACAGC AGAAGCTATG
CCCAAGGGAC CAGCAGGGAC CATGGTATCC CTAGCTCCTC AGCCATCTCA GGACACAGGG
CGGGCACAAG ATCAGAACAC GCTATACTAC TATGGCCAGA TCCACATGTA TGAACAGAAT
GGAGGCTGCC CAGCCGTGCA GCCCCAGCCG CCACAACCAC AAGCTTGCTC AGACAGTATC
CAGCCTGAGC CTTTGCCTTC ACCGGGAGTC AACCAGGTGT CTAGCACCGT GGACTCCCAG
CTCCTGGAGC CCCCCCAGAT TGACTTTGAT GCCATCATGG ATGATGGTGA TCACTCGAGT
TTGTTTTCTG GTGCACTGAG CCCAACCCTT CTCCACAATC TCTCCCAGAA TTCCTCACGC
CTCACCACAC CCCGGAATTC CTTGACACTG CCCTCCATCC CTGCGGGCAT CAGCAACATG
GCCGTGGGCG ACATGAGTTC CATGCTCACC AGCCTGGCTG AAGAGAGCAA GTTTTTAAAC
ATGATGACCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu27904
Clone ID Related Accession (Same CDS sequence) XM_006529141 , XM_011247924
Accession Version XM_006529141.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product zinc finger protein GLI2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_078297.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:568908146. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACT CTGTCCACAG TGTACACGGG 
CCTCCCACCC TAAGTGGCAG CCCTGTCATC TCAGATATCT CCTTGATACG ACTTTCTCCA
CACCCTGCTG GCCCTGGAGA GTCACCCTTC AGCGCCCACC ACCCCTACGT GAACCCCCAT
ATGGAGCACT ACCTCCGGTC TGTGCACAGC AGCCCCACAC TCTCAATGAT CTCTGCCGCC
AGGGGCCTCA GCCCTGCTGA TGTGGCCCAC GAACATCTGA AAGAGAGGGG ACTCTTTAGC
CTCGCAGCCC CAGGCACCAA CCCTTCAGAC TATTACCACC AGATGACCCT CATGGCAAGC
CACCCCACCC CTTATGGGGA CCTTCTAATG CAGAGCGGGG GTGCTGCTAG CGCACCCCAT
CTCCATGACT ACCTCAACCC TGTGGATGCA TCACGATTCT CTAGTCCACG TGTGACCCCA
CGACTGAGCC GCAAGCGGGC TCTGTCCATC TCCCCGCTCT CAGATGCCAG CCTCGACCTA
CAACGCATGA TTCGGACCTC TCCCAACTCG CTGGTAGCTT ACATCAACAA CTCCAGGAGC
AGCTCAGCAG CCAGTGGCTC TTATGGACAT CTGTCTGCTG GTGCCCTCAG CCCAGCCTTC
ACTTTTCCCC ACCCCATCAA TCCTGTGGCC TACCAGCAGA TCCTGAGCCA GCAGCGGGGC
CTGGGCTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CTTCACCCAC CTTCTTGGCC
CAGCAGCCCA TGACTCTCAC CTCCATCAGC ACCATGCCTA CCCAACTCAG CAGCAGTAGC
AGCAACTGTC TAAATGATGC CAACCAGAAC AAGCAGAACA GCGAGTCAGC TGTGAGCAGC
ACCGTGAACC CCATCACCAT TCATAAGCGG AGCAAGGTCA AGACTGAGGC TGAGGGCCTG
CGTCCAGCAT CCCCGCTTGG ACTGACACAG GAGCAGCTGG CCGATCTCAA GGAAGACCTG
GACAGGGATG ACTGTAAGCA GGAGGCCGAG GTGGTCATCT ACGAGACCAA CTGCCACTGG
GCAGACTGCA CCAAGGAGTA TGACACACAG GAGCAGCTGG TGCATCATAT CAACAATGAA
CACATCCACG GGGAGAAGAA GGAGTTCGTG TGCCGCTGGC AGGCCTGCAC GAGAGAGCAG
AAGCCCTTCA AGGCCCAGTA CATGCTGGTT GTTCACATGC GCAGACACAC GGGTGAGAAG
CCACACAAGT GCACGTTCGA AGGCTGTTCC AAGGCCTACT CTCGCCTGGA GAACCTGAAG
ACACACCTGC GTTCACACAC AGGAGAGAAG CCATATGTGT GTGAACACGA AGGCTGTAAC
AAAGCCTTCT CCAATGCCTC AGACCGCGCC AAGCACCAGA ACCGCACTCA CTCCAATGAG
AAACCCTACA TCTGCAAGAT CCCAGGCTGC ACCAAGAGGT ACACAGACCC CAGCTCACTC
CGCAAGCATG TGAAGACTGT CCATGGGCCA GACGCCCATG TCACCAAGAA ACAGCGTAAT
GATGTGCATG TCCGTGCTCC ACTGCTCAAG GAGAATGGGG ATAATGAGGC CAGCGCCGAG
CCAGGTGGCC GGGGACCTGA GGAGAGTGTG GAGGCCAGTA GCACCAGCCA CACTGTGGAG
GACTGCCTAC ATATCAAAGC CATCAAGACA GAGAGCTCCG GGCTTTGTCA GTCCAGCCCC
GGGGCCCAGT CATCCTGCAG CAGCGAGCCC TCTCCCCTGG GCAGTGCCCC CAACAATGAC
AGTGGCATGG AGATGCCGGG GACAGGGCCT GGGAGTCTGG GAGACCTGAC AGCACTGGCT
GACACGTGTC CAGGAGCTGA CACCTCAGCC CTGGCTGCAC CCTCCACTGG TGGCCTGCAG
CTGCGCAAAC ACATGAGCAC CGTGCATCGC TTTGAGCAGC TGAAGAGAGA GAAGCTCAAG
TCACTGAAGG ATTCCTGCTC GTGGGCCGGC CCAGCTCCAC ACACCCGCAA CACCAAGCTG
CCTCCCCTTC CAGTCAATGG TTCTGTCCTG GAAAACTTCA ACAATACAGG GGGCGGTGGA
CCGGCAGGAC TGCTGCCCAG CCAGCGGCTA CCAGAGCTGA CCGAAGTGAC GATGCTGAGC
CAGCTGCAGG AACGAAGAGA CAGCTCCACC AGCACCATGA GCTCGGCCTA CACTGTGAGC
CGCCGCTCCT CTGGCATCTC CCCATACTTC TCTAGCCGTC GCTCCAGCGA GGCTTCGCCT
CTCGGTGGCC TACGCCCGCA CAACGCCAGC TCAGCAGACT CCTATGACCC CATCTCCACA
GATGCCTCTC GGCGCTCCAG TGAAGCCAGC CAGTGCAGTG GCGGTGGCCC AGGGCTGCTC
AACCTCACAC CTGCGCAGCA GTACAACCTG CGTGCCAAGT ACGCAGCGGC CACAGGTGGA
CCACCGCCCA CGCCACTGCC GGGCCTCGAT CGTGTAAGCC TTCGTACCCG CCTGGCCTTG
CTGGATGCTC CTGAGCGTGC ACTTCCTGGT GCCTGCCCAC ATCCACTGGG GCCACGGCGT
GGCAGCGATG GGCCTACCTA TAGCCATGGT CATGGCCATG GCTACGCAGG TGCGGCTCCA
GCATTCCCCC ACGAGGGGCC AAACAGCAGC ACACGGCGGG CCAGCGACCC TGTGCGGCGC
CCTGACCCCC TTATTCTGCC TCGAGTGCAA CGTTTCCACA GTACCCACAA CATGAATCCA
GGTTCACTGC CACCCTGCGC TGATCGGCGT GGCCTGCACG TACAGAGCCA CCCCAGCGTA
GACAGCAACC TGACCCGCAA CGCCTACTCT CCCAGACCCC CTAGCATCAA TGAGAACGTG
GTGATGGAGG CCGTGGCTGC TGGGGTAGAC GGCCCAGGGC TAGAGTGCGA CCTGGGGCTG
GTGGAGGATG AGCTGGTGCT GCCAGATGAT GTGGTACAGT ACATCAAGGC TCACACCGGT
GGTACCTTGG ATGACGGCAT TCGGCAGGGG TATCCCACAG AAGGTACTGG CTTCCCCGAG
AACTCTAAGC TGCCCAGTCC TGGGCTACAA GGCCACCGCA GGCTAGCAGC TGCCGACTCC
AACATGGGTC CTTCTGCTCC TGGACTCGGG GGCTGCCAGC TGAGCTACAG CCCCTCCTCC
AACCTCAACA AGAGCAACAT GCCTGTGCAG TGGAATGAGG TGAGTTCTGG CACCGTGGAT
GCCCTGCCTA CCCAGGTGAA GCCACCTCCT TTCCCTCACA GCAACCTGGC TGTGGTCCAA
CAGAAGCCAG CCTTTGGCCA GTATCCAGGA TATAATCCAC AATCCGTGCA GAGCAGCTCC
GGAGGTCTAG ACAGCACCCA GCCGCACCTA CAGCTTCGAG GAGCCCCCTC TGCATCAAGA
GGGAGCTACA CGCAACAGCC TCGACAGCCA GCTGCAGGCA GTCAGTGCCT GGGTATGAGT
GCGGCCATGA GCCCGCAGGC CAGCTACAGC CAAGCCCACC CCCAGCTGAG CCCAAACATT
GTCAGCGGAT CTCTGAACCA GTTTTCTCCC TCCTGCAGCA ATATGGCAGC CAAGCCCAGC
CACCTGGGAC TCCCTCAGCA AATGGAAGTT GTCCCCAATG CCACCATCAT GAATGGCCAT
CAACGGGAGC ACGGGGTCCC CAATTCATCC CTGGCTGCGG TGTCACAACC TCACCCAGTC
CTGAGCTATC CCCAGCAGGA CAGCTACCAA CAGGGCTCCA ACCTTCTGTC ATCCCATCAG
CCTGGCTTCA TGGAGTCCCA GCAGAACGCG GGCTTTGGTC TCATGCAGCC TCGGCCACCC
CTGGAACCCA ACACGGCCAG CCGTCACCGT GGAGTACGTT CTGGGCAACA GCAGTTGTAT
GCCAGGACCA CTGGCCAAGC CATGGTCACA TCAGCCAACC AAGAGACAGC AGAAGCTATG
CCCAAGGGAC CAGCAGGGAC CATGGTATCC CTAGCTCCTC AGCCATCTCA GGACACAGGG
CGGGCACAAG ATCAGAACAC GCTATACTAC TATGGCCAGA TCCACATGTA TGAACAGAAT
GGAGGCTGCC CAGCCGTGCA GCCCCAGCCG CCACAACCAC AAGCTTGCTC AGACAGTATC
CAGCCTGAGC CTTTGCCTTC ACCGGGAGTC AACCAGGTGT CTAGCACCGT GGACTCCCAG
CTCCTGGAGC CCCCCCAGAT TGACTTTGAT GCCATCATGG ATGATGGTGA TCACTCGAGT
TTGTTTTCTG GTGCACTGAG CCCAACCCTT CTCCACAATC TCTCCCAGAA TTCCTCACGC
CTCACCACAC CCCGGAATTC CTTGACACTG CCCTCCATCC CTGCGGGCAT CAGCAACATG
GCCGTGGGCG ACATGAGTTC CATGCTCACC AGCCTGGCTG AAGAGAGCAA GTTTTTAAAC
ATGATGACCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006529204.1
CDS164..4615
Misc Feature(1)1364..1591(+)
Misc Feature(2)1364..1411(+)
Misc Feature(3)1385..1468(+)
Misc Feature(4)1397..1411(+)
Misc Feature(5)1433..1501(+)
Misc Feature(6)1433..1498(+)
Misc Feature(7)1475..1558(+)
Misc Feature(8)1523..1594(+)
Misc Feature(9)1523..1594(+)
Misc Feature(10)1532..1594(+)
Misc Feature(11)1538..1594(+)
Misc Feature(12)1544..1681(+)
Misc Feature(13)1616..1681(+)
Misc Feature(14)1616..1672(+)
Translation

Target ORF information:

RefSeq Version XM_006529141
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus GLI-Kruppel family member GLI2 (Gli2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006529141

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAGACACC AGGAGGGAAG GTACCATTAT GACCCTCACT CTGTCCACAG TGTACACGGG 
CCTCCCACCC TAAGTGGCAG CCCTGTCATC TCAGATATCT CCTTGATACG ACTTTCTCCA
CACCCTGCTG GCCCTGGAGA GTCACCCTTC AGCGCCCACC ACCCCTACGT GAACCCCCAT
ATGGAGCACT ACCTCCGGTC TGTGCACAGC AGCCCCACAC TCTCAATGAT CTCTGCCGCC
AGGGGCCTCA GCCCTGCTGA TGTGGCCCAC GAACATCTGA AAGAGAGGGG ACTCTTTAGC
CTCGCAGCCC CAGGCACCAA CCCTTCAGAC TATTACCACC AGATGACCCT CATGGCAAGC
CACCCCACCC CTTATGGGGA CCTTCTAATG CAGAGCGGGG GTGCTGCTAG CGCACCCCAT
CTCCATGACT ACCTCAACCC TGTGGATGCA TCACGATTCT CTAGTCCACG TGTGACCCCA
CGACTGAGCC GCAAGCGGGC TCTGTCCATC TCCCCGCTCT CAGATGCCAG CCTCGACCTA
CAACGCATGA TTCGGACCTC TCCCAACTCG CTGGTAGCTT ACATCAACAA CTCCAGGAGC
AGCTCAGCAG CCAGTGGCTC TTATGGACAT CTGTCTGCTG GTGCCCTCAG CCCAGCCTTC
ACTTTTCCCC ACCCCATCAA TCCTGTGGCC TACCAGCAGA TCCTGAGCCA GCAGCGGGGC
CTGGGCTCAG CCTTTGGACA CACACCACCC CTGATCCAGC CTTCACCCAC CTTCTTGGCC
CAGCAGCCCA TGACTCTCAC CTCCATCAGC ACCATGCCTA CCCAACTCAG CAGCAGTAGC
AGCAACTGTC TAAATGATGC CAACCAGAAC AAGCAGAACA GCGAGTCAGC TGTGAGCAGC
ACCGTGAACC CCATCACCAT TCATAAGCGG AGCAAGGTCA AGACTGAGGC TGAGGGCCTG
CGTCCAGCAT CCCCGCTTGG ACTGACACAG GAGCAGCTGG CCGATCTCAA GGAAGACCTG
GACAGGGATG ACTGTAAGCA GGAGGCCGAG GTGGTCATCT ACGAGACCAA CTGCCACTGG
GCAGACTGCA CCAAGGAGTA TGACACACAG GAGCAGCTGG TGCATCATAT CAACAATGAA
CACATCCACG GGGAGAAGAA GGAGTTCGTG TGCCGCTGGC AGGCCTGCAC GAGAGAGCAG
AAGCCCTTCA AGGCCCAGTA CATGCTGGTT GTTCACATGC GCAGACACAC GGGTGAGAAG
CCACACAAGT GCACGTTCGA AGGCTGTTCC AAGGCCTACT CTCGCCTGGA GAACCTGAAG
ACACACCTGC GTTCACACAC AGGAGAGAAG CCATATGTGT GTGAACACGA AGGCTGTAAC
AAAGCCTTCT CCAATGCCTC AGACCGCGCC AAGCACCAGA ACCGCACTCA CTCCAATGAG
AAACCCTACA TCTGCAAGAT CCCAGGCTGC ACCAAGAGGT ACACAGACCC CAGCTCACTC
CGCAAGCATG TGAAGACTGT CCATGGGCCA GACGCCCATG TCACCAAGAA ACAGCGTAAT
GATGTGCATG TCCGTGCTCC ACTGCTCAAG GAGAATGGGG ATAATGAGGC CAGCGCCGAG
CCAGGTGGCC GGGGACCTGA GGAGAGTGTG GAGGCCAGTA GCACCAGCCA CACTGTGGAG
GACTGCCTAC ATATCAAAGC CATCAAGACA GAGAGCTCCG GGCTTTGTCA GTCCAGCCCC
GGGGCCCAGT CATCCTGCAG CAGCGAGCCC TCTCCCCTGG GCAGTGCCCC CAACAATGAC
AGTGGCATGG AGATGCCGGG GACAGGGCCT GGGAGTCTGG GAGACCTGAC AGCACTGGCT
GACACGTGTC CAGGAGCTGA CACCTCAGCC CTGGCTGCAC CCTCCACTGG TGGCCTGCAG
CTGCGCAAAC ACATGAGCAC CGTGCATCGC TTTGAGCAGC TGAAGAGAGA GAAGCTCAAG
TCACTGAAGG ATTCCTGCTC GTGGGCCGGC CCAGCTCCAC ACACCCGCAA CACCAAGCTG
CCTCCCCTTC CAGTCAATGG TTCTGTCCTG GAAAACTTCA ACAATACAGG GGGCGGTGGA
CCGGCAGGAC TGCTGCCCAG CCAGCGGCTA CCAGAGCTGA CCGAAGTGAC GATGCTGAGC
CAGCTGCAGG AACGAAGAGA CAGCTCCACC AGCACCATGA GCTCGGCCTA CACTGTGAGC
CGCCGCTCCT CTGGCATCTC CCCATACTTC TCTAGCCGTC GCTCCAGCGA GGCTTCGCCT
CTCGGTGGCC TACGCCCGCA CAACGCCAGC TCAGCAGACT CCTATGACCC CATCTCCACA
GATGCCTCTC GGCGCTCCAG TGAAGCCAGC CAGTGCAGTG GCGGTGGCCC AGGGCTGCTC
AACCTCACAC CTGCGCAGCA GTACAACCTG CGTGCCAAGT ACGCAGCGGC CACAGGTGGA
CCACCGCCCA CGCCACTGCC GGGCCTCGAT CGTGTAAGCC TTCGTACCCG CCTGGCCTTG
CTGGATGCTC CTGAGCGTGC ACTTCCTGGT GCCTGCCCAC ATCCACTGGG GCCACGGCGT
GGCAGCGATG GGCCTACCTA TAGCCATGGT CATGGCCATG GCTACGCAGG TGCGGCTCCA
GCATTCCCCC ACGAGGGGCC AAACAGCAGC ACACGGCGGG CCAGCGACCC TGTGCGGCGC
CCTGACCCCC TTATTCTGCC TCGAGTGCAA CGTTTCCACA GTACCCACAA CATGAATCCA
GGTTCACTGC CACCCTGCGC TGATCGGCGT GGCCTGCACG TACAGAGCCA CCCCAGCGTA
GACAGCAACC TGACCCGCAA CGCCTACTCT CCCAGACCCC CTAGCATCAA TGAGAACGTG
GTGATGGAGG CCGTGGCTGC TGGGGTAGAC GGCCCAGGGC TAGAGTGCGA CCTGGGGCTG
GTGGAGGATG AGCTGGTGCT GCCAGATGAT GTGGTACAGT ACATCAAGGC TCACACCGGT
GGTACCTTGG ATGACGGCAT TCGGCAGGGG TATCCCACAG AAGGTACTGG CTTCCCCGAG
AACTCTAAGC TGCCCAGTCC TGGGCTACAA GGCCACCGCA GGCTAGCAGC TGCCGACTCC
AACATGGGTC CTTCTGCTCC TGGACTCGGG GGCTGCCAGC TGAGCTACAG CCCCTCCTCC
AACCTCAACA AGAGCAACAT GCCTGTGCAG TGGAATGAGG TGAGTTCTGG CACCGTGGAT
GCCCTGCCTA CCCAGGTGAA GCCACCTCCT TTCCCTCACA GCAACCTGGC TGTGGTCCAA
CAGAAGCCAG CCTTTGGCCA GTATCCAGGA TATAATCCAC AATCCGTGCA GAGCAGCTCC
GGAGGTCTAG ACAGCACCCA GCCGCACCTA CAGCTTCGAG GAGCCCCCTC TGCATCAAGA
GGGAGCTACA CGCAACAGCC TCGACAGCCA GCTGCAGGCA GTCAGTGCCT GGGTATGAGT
GCGGCCATGA GCCCGCAGGC CAGCTACAGC CAAGCCCACC CCCAGCTGAG CCCAAACATT
GTCAGCGGAT CTCTGAACCA GTTTTCTCCC TCCTGCAGCA ATATGGCAGC CAAGCCCAGC
CACCTGGGAC TCCCTCAGCA AATGGAAGTT GTCCCCAATG CCACCATCAT GAATGGCCAT
CAACGGGAGC ACGGGGTCCC CAATTCATCC CTGGCTGCGG TGTCACAACC TCACCCAGTC
CTGAGCTATC CCCAGCAGGA CAGCTACCAA CAGGGCTCCA ACCTTCTGTC ATCCCATCAG
CCTGGCTTCA TGGAGTCCCA GCAGAACGCG GGCTTTGGTC TCATGCAGCC TCGGCCACCC
CTGGAACCCA ACACGGCCAG CCGTCACCGT GGAGTACGTT CTGGGCAACA GCAGTTGTAT
GCCAGGACCA CTGGCCAAGC CATGGTCACA TCAGCCAACC AAGAGACAGC AGAAGCTATG
CCCAAGGGAC CAGCAGGGAC CATGGTATCC CTAGCTCCTC AGCCATCTCA GGACACAGGG
CGGGCACAAG ATCAGAACAC GCTATACTAC TATGGCCAGA TCCACATGTA TGAACAGAAT
GGAGGCTGCC CAGCCGTGCA GCCCCAGCCG CCACAACCAC AAGCTTGCTC AGACAGTATC
CAGCCTGAGC CTTTGCCTTC ACCGGGAGTC AACCAGGTGT CTAGCACCGT GGACTCCCAG
CTCCTGGAGC CCCCCCAGAT TGACTTTGAT GCCATCATGG ATGATGGTGA TCACTCGAGT
TTGTTTTCTG GTGCACTGAG CCCAACCCTT CTCCACAATC TCTCCCAGAA TTCCTCACGC
CTCACCACAC CCCGGAATTC CTTGACACTG CCCTCCATCC CTGCGGGCAT CAGCAACATG
GCCGTGGGCG ACATGAGTTC CATGCTCACC AGCCTGGCTG AAGAGAGCAA GTTTTTAAAC
ATGATGACCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

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Hum. Mol. Genet. 24 (10), 2808-2825 (2015)
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Development 142 (9), 1651-1660 (2015)
Liu J, Zeng H and Liu A.


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FASEB J. 29 (5), 2059-2069 (2015)
La Sala G, Marazziti D, Di Pietro C, Golini E, Matteoni R and Tocchini-Valentini GP.


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Cell-autonomous activation of Hedgehog signaling inhibits brown adipose tissue development
Proc. Natl. Acad. Sci. U.S.A. 112 (16), 5069-5074 (2015)
Nosavanh L, Yu DH, Jaehnig EJ, Tong Q, Shen L and Chen MH.


book

Cloning and sequencing of the mouse Gli2 gene: localization to the Dominant hemimelia critical region
Genomics 39 (2), 205-215 (1997)
Hughes DC, Allen J, Morley G, Sutherland K, Ahmed W, Prosser J, Lettice L, Allan G, Mattei MG, Farrall M and Hill RE.


book

Specific and redundant functions of Gli2 and Gli3 zinc finger genes in skeletal patterning and development
Development 124 (1), 113-123 (1997)
Mo R, Freer AM, Zinyk DL, Crackower MA, Michaud J, Heng HH, Chik KW, Shi XM, Tsui LC, Cheng SH, Joyner AL and Hui C.


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A role for En-2 and other murine homologues of Drosophila segment polarity genes in regulating positional information in the developing cerebellum
Development 121 (12), 3935-3945 (1995)
Millen KJ, Hui CC and Joyner AL.


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Expression of three mouse homologs of the Drosophila segment polarity gene cubitus interruptus, Gli, Gli-2, and Gli-3, in ectoderm- and mesoderm-derived tissues suggests multiple roles during postimplantation development
Dev. Biol. 162 (2), 402-413 (1994)
Hui CC, Slusarski D, Platt KA, Holmgren R and Joyner AL.


book

A mouse model of greig cephalopolysyndactyly syndrome: the extra-toesJ mutation contains an intragenic deletion of the Gli3 gene
Nat. Genet. 3 (3), 241-246 (1993)
Hui CC and Joyner AL.


 
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