Hmgcr cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Hmgcr
Entrez Gene ID	15357
Full Name	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Synonyms	HMG-CoAR,Red
General protein information	Preferred Names 3-hydroxy-3-methylglutaryl-Coenzyme A reductase Names 3-hydroxy-3-methylglutaryl-coenzyme A reductase 3-hydroxy-3-methylglutaryl-CoA reductase HMG-CoA reductase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 D1\|13 50.65 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006517531.1	XP_006517594.1	3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform X2
NM_008255.2	NP_032281.2	3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform 1
NM_001360166.1	NP_001347095.1	3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform 2
NM_001360165.1	NP_001347094.1	3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform 1
XM_006517530.1	XP_006517593.1	3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HMGCR	NP_000850.1
Gallus gallus (chicken)	HMGCR	NP_989816.1
Caenorhabditis elegans (roundworm)	hmgr-1	NP_498626.2
Saccharomyces cerevisiae (baker's yeast)	HMG2	NP_013555.1
Kluyveromyces lactis	KLLA0B04642g	XP_451740.1
Pan troglodytes (chimpanzee)	HMGCR	XP_001148405.2
Bos taurus (cattle)	HMGCR	NP_001099083.1
Xenopus tropicalis (tropical clawed frog)	hmgcr	XP_004910453.1
	HMGCR	XP_002804463.1
Canis lupus familiaris (dog)	HMGCR	XP_536323.3
Drosophila melanogaster (fruit fly)	Hmgcr	NP_732900.1
Magnaporthe oryzae (rice blast fungus)	MGG_08975	XP_003714014.1
Neurospora crassa	NCU00712	XP_964546.1
Arabidopsis thaliana (thale cress)	HMG1	NP_177775.2
Oryza sativa (rice)	Os09g0492700	NP_001063541.1
Oryza sativa (rice)	Os08g0512700	NP_001062221.1
Mus musculus (house mouse)	Hmgcr	NP_032281.2
Rattus norvegicus (Norway rat)	Hmgcr	NP_037266.2
Danio rerio (zebrafish)	hmgcra	NP_001073446.1
Arabidopsis thaliana (thale cress)	HMG2	NP_179329.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002288	XP_307890.5
Saccharomyces cerevisiae (baker's yeast)	HMG1	NP_013636.1
Eremothecium gossypii	AGOS_AER152W	NP_985010.2
Schizosaccharomyces pombe (fission yeast)	hmg1	NP_588235.1

Gene Ontology

The following Hmgcr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hmgcr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14982	NM_008255.2 Latest version!	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu14982	NM_001360165.1 Latest version!	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu48955	NM_001360166.1 Latest version!	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu14982	XM_006517531.1 Latest version!	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu48955	XM_006517530.1 Latest version!	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hmgcr ( NM_008255.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032281.2 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14982
Clone ID Related Accession (Same CDS sequence)	XM_006517531.1 , NM_008255.2 , NM_001360165.1
Accession Version	NM_008255.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154851.3, BY124492.1, AK159899.1, AK048784.1, AK079302.1 and BM899288.1. On Nov 16, 2007 this sequence version replaced NM_008255.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK159899.1, BC085083.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGTCAA GACTTTTCCG GATGCATGGC CTCTTCGTGG CCTCCCACCC CTGGGAAGTT 
ATTGTGGGAA CAGTGACACT TACAATCTGT ATGATGTCCA TGAACATGTT CACCGGCAAC 
AACAAGATCT GTGGCTGGAA TTATGAGTGC CCTAAATTTG AAGAGGACGT GCTGAGCAGC 
GACATCATCA TCCTGACGAT AACGCGGTGC ATCGCCATCC TGTACATTTA CTTCCAGTTC 
CAGAACCTAC GGCAGCTTGG GTCCAAGTAC ATTCTGGGTA TTGCTGGCCT CTTCACAATT 
TTCTCAAGCT TTGTCTTCAG TACAGTCGTC ATTCATTTCC TCGACAAAGA ACTGACAGGC 
TTAAATGAAG CTTTGCCCTT TTTTCTGCTC TTGATTGACC TTTCTAGAGC GAGTGCATTA 
GCAAAGTTTG CCCTCAGTTC AAATTCACAG GATGAAGTAA GGGAAAATAT AGCTCGTGGA 
ATGGCAATCC TGGGCCCCAC ATTCACTCTT GACGCTCTTG TGGAATGCCT TGTGATTGGA 
GTTGGCACCA TGTCAGGCGT CCGCCAGCTG GAGATCATGT GCTGCTTCGG CTGCATGTCA 
GTGTTGGCCA ACTACTTTGT GTTCATGACA TTCTTCCCGG CCTGTGTGTC GCTGGTCCTA 
GAGCTTTCTC GTGAAAGCCG TGAGGGTCGT CCAATTTGGC AGCTCAGCCA TTTTGCCAGA 
GTTTTAGAAG AAGAAGAAAA TAAACCAAAC CCCGTAACCC AAAGGGTCAA GATGATTATG 
TCTTTAGGCT TGGTCCTTGT TCACGCTCAT AGTCGCTGGA TAGCTGATCC TTCTCCTCAG 
AACAGCACAG CGGAGCAGGC TAAGGTTTCC TTGGGTCTGG ACGAAGATGT GTCCAAGAGA 
ATTGAACCAA GTGTTTCTCT CTGGCAGTTT TATCTCTCCA AGATGATCAG CATGGACATT 
GAGCAAGTGA TTACCCTGAG TTTAGCCTTC CTTTTGGCTG TCAAGTATAT TTTCTTTGAA 
CAAGCAGAGA CAGAATCGAC ACTGTCATTA AAAAATCCTA TCACGTCTCC CGTCGTGACC 
TCAAAGAAAG CTCAAGACAA CTGTTGTAGA CGTGAGCCTC TGCTTGTGAG AAGGAACCAG 
AAGCTTTCGT CAGTAGAGGA GGATCCAGGA GCGAACCAAG AGAGAAAAGT TGAGGTTATA 
AAACCATTAG TGGTGGAAGC AGAGACTACA AGCAGAGCTA CGTTTGTGCT TGGGGCTTCT 
GTAGCCAGCC CTCCATCCGC CCTGGGGACA CAGGAGCCTG GAATTGAACT CCCCATCGAG 
CCACGACCTA ATGAAGAATG TCTGCAGATA CTGGAGAATG CAGAGAAAGG TGCAAAGTTC 
CTTAGTGATG CAGAGATCAT CCAGTTGGTC AATGCCAAGC ATATCCCAGC TTACAAATTG 
GAAACTCTAA TGGAAACTCA TGAACGTGGT GTGTCTATTC GCCGGCAGCT CCTCTCCACA 
AAGCTTGCAG AGCCGTCCTC TCTACAGTAC CTGCCTTACA GAGATTATAA TTATTCCTTG 
GTGATGGGAG CTTGCTGTGA GAATGTGATC GGATATATGC CCATCCCTGT TGGAGTGGCA 
GGGCCTCTGT GCCTGGATGG GAAGGAGTAC CAGGTGCCGA TGGCAACGAC GGAAGGCTGT 
CTTGTGGCCA GCACTAACAG AGGCTGCAGA GCCATAAGCC TTGGTGGAGG TGCCAGCAGC 
CGGGTCCTTG CAGATGGGAT GACTCGAGGC CCAGTGGTGC GTCTTCCACG TGCTTGTGAC 
TCTGCAGAAG TAAAGACCTG GCTTGAAACA CCTGAAGGGT TTGCAGTGAT AAAGGAGGCC 
TTTGATAGCA CCAGCAGATT TGCTCGTCTA CAGAAACTCC ACGTGACGAT GGCAGGACGC 
AACCTCTATA TCCGTTTCCA GTCCAGGACG GGGGACGCCA TGGGCATGAA CATGATCTCT 
AAGGGTACGG AGAAAGCACT GCTGAAGCTT CAGGAGTTCT TTCCTGACAT GCAGATTCTG 
GCAGTCAGTG GGAACTATTG CACCGACAAG AAGCCTGCTG CCATAAATTG GATCGAAGGA 
CGAGGAAAGA CTGTGGTTTG TGAAGCCGTC ATTCCAGCCA AGGTGGTGAG AGAGGTGTTA 
AAGACAACTA CGGAAGCTAT GGTTGACGTA AACATTAACA AGAATCTTGT GGGCTCGGCC 
ATGGCTGGGA GCATAGGCGG CTACAACGCC CACGCAGCAA ACATTGTCAC TGCTATCTAC 
ATCGCATGTG GCCAGGATGC AGCACAGAAT GTGGGGAGTT CAAACTGTAT TACTTTAATG 
GAAGCCAGTG GTCCCACAAA TGAAGACTTA TATATCAGCT GCACCATGCC ATCGATAGAG 
ATAGGAACCG TGGGTGGTGG GACCAACCTT CTACCTCAGC AAGCCTGCCT GCAGATGCTA 
GGTGTTCAAG GAGCATGCAA AGACAATCCT GGAGAAAACG CACGGCAGCT TGCCCGAATT 
GTATGTGGCA CTGTGATGGC TGGTGAGCTG TCCTTGATGG CAGCCTTGGC AGCAGGACAT 
CTTGTCAGAA GTCACATGGT TCACAACAGA TCAAAGATAA ATTTACAAGA TCTTCAAGGA 
ACGTGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032281.2
CDS	150..2813
Translation	MLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASALAKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIADPSPQNSTAEQAKVSLGLDEDVSKRIEPSVSLWQFYLSKMISMDIEQVITLSLAFLLAVKYIFFEQAETESTLSLKNPITSPVVTSKKAQDNCCRREPLLVRRNQKLSSVEEDPGANQERKVEVIKPLVVEAETTSRATFVLGASVASPPSALGTQEPGIELPIEPRPNEECLQILENAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSTKLAEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDGKEYQVPMATTEGCLVASTNRGCRAISLGGGASSRVLADGMTRGPVVRLPRACDSAEVKTWLETPEGFAVIKEAFDSTSRFARLQKLHVTMAGRNLYIRFQSRTGDAMGMNMISKGTEKALLKLQEFFPDMQILAVSGNYCTDKKPAAINWIEGRGKTVVCEAVIPAKVVREVLKTTTEAMVDVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVRSHMVHNRSKINLQDLQGTCTKKAA

Target ORF information:

RefSeq Version	NM_008255.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008255.2

ORF Insert Sequence:

ATGTTGTCAA GACTTTTCCG GATGCATGGC CTCTTCGTGG CCTCCCACCC CTGGGAAGTT 
ATTGTGGGAA CAGTGACACT TACAATCTGT ATGATGTCCA TGAACATGTT CACCGGCAAC 
AACAAGATCT GTGGCTGGAA TTATGAGTGC CCTAAATTTG AAGAGGACGT GCTGAGCAGC 
GACATCATCA TCCTGACGAT AACGCGGTGC ATCGCCATCC TGTACATTTA CTTCCAGTTC 
CAGAACCTAC GGCAGCTTGG GTCCAAGTAC ATTCTGGGTA TTGCTGGCCT CTTCACAATT 
TTCTCAAGCT TTGTCTTCAG TACAGTCGTC ATTCATTTCC TCGACAAAGA ACTGACAGGC 
TTAAATGAAG CTTTGCCCTT TTTTCTGCTC TTGATTGACC TTTCTAGAGC GAGTGCATTA 
GCAAAGTTTG CCCTCAGTTC AAATTCACAG GATGAAGTAA GGGAAAATAT AGCTCGTGGA 
ATGGCAATCC TGGGCCCCAC ATTCACTCTT GACGCTCTTG TGGAATGCCT TGTGATTGGA 
GTTGGCACCA TGTCAGGCGT CCGCCAGCTG GAGATCATGT GCTGCTTCGG CTGCATGTCA 
GTGTTGGCCA ACTACTTTGT GTTCATGACA TTCTTCCCGG CCTGTGTGTC GCTGGTCCTA 
GAGCTTTCTC GTGAAAGCCG TGAGGGTCGT CCAATTTGGC AGCTCAGCCA TTTTGCCAGA 
GTTTTAGAAG AAGAAGAAAA TAAACCAAAC CCCGTAACCC AAAGGGTCAA GATGATTATG 
TCTTTAGGCT TGGTCCTTGT TCACGCTCAT AGTCGCTGGA TAGCTGATCC TTCTCCTCAG 
AACAGCACAG CGGAGCAGGC TAAGGTTTCC TTGGGTCTGG ACGAAGATGT GTCCAAGAGA 
ATTGAACCAA GTGTTTCTCT CTGGCAGTTT TATCTCTCCA AGATGATCAG CATGGACATT 
GAGCAAGTGA TTACCCTGAG TTTAGCCTTC CTTTTGGCTG TCAAGTATAT TTTCTTTGAA 
CAAGCAGAGA CAGAATCGAC ACTGTCATTA AAAAATCCTA TCACGTCTCC CGTCGTGACC 
TCAAAGAAAG CTCAAGACAA CTGTTGTAGA CGTGAGCCTC TGCTTGTGAG AAGGAACCAG 
AAGCTTTCGT CAGTAGAGGA GGATCCAGGA GCGAACCAAG AGAGAAAAGT TGAGGTTATA 
AAACCATTAG TGGTGGAAGC AGAGACTACA AGCAGAGCTA CGTTTGTGCT TGGGGCTTCT 
GTAGCCAGCC CTCCATCCGC CCTGGGGACA CAGGAGCCTG GAATTGAACT CCCCATCGAG 
CCACGACCTA ATGAAGAATG TCTGCAGATA CTGGAGAATG CAGAGAAAGG TGCAAAGTTC 
CTTAGTGATG CAGAGATCAT CCAGTTGGTC AATGCCAAGC ATATCCCAGC TTACAAATTG 
GAAACTCTAA TGGAAACTCA TGAACGTGGT GTGTCTATTC GCCGGCAGCT CCTCTCCACA 
AAGCTTGCAG AGCCGTCCTC TCTACAGTAC CTGCCTTACA GAGATTATAA TTATTCCTTG 
GTGATGGGAG CTTGCTGTGA GAATGTGATC GGATATATGC CCATCCCTGT TGGAGTGGCA 
GGGCCTCTGT GCCTGGATGG GAAGGAGTAC CAGGTGCCGA TGGCAACGAC GGAAGGCTGT 
CTTGTGGCCA GCACTAACAG AGGCTGCAGA GCCATAAGCC TTGGTGGAGG TGCCAGCAGC 
CGGGTCCTTG CAGATGGGAT GACTCGAGGC CCAGTGGTGC GTCTTCCACG TGCTTGTGAC 
TCTGCAGAAG TAAAGACCTG GCTTGAAACA CCTGAAGGGT TTGCAGTGAT AAAGGAGGCC 
TTTGATAGCA CCAGCAGATT TGCTCGTCTA CAGAAACTCC ACGTGACGAT GGCAGGACGC 
AACCTCTATA TCCGTTTCCA GTCCAGGACG GGGGACGCCA TGGGCATGAA CATGATCTCT 
AAGGGTACGG AGAAAGCACT GCTGAAGCTT CAGGAGTTCT TTCCTGACAT GCAGATTCTG 
GCAGTCAGTG GGAACTATTG CACCGACAAG AAGCCTGCTG CCATAAATTG GATCGAAGGA 
CGAGGAAAGA CTGTGGTTTG TGAAGCCGTC ATTCCAGCCA AGGTGGTGAG AGAGGTGTTA 
AAGACAACTA CGGAAGCTAT GGTTGACGTA AACATTAACA AGAATCTTGT GGGCTCGGCC 
ATGGCTGGGA GCATAGGCGG CTACAACGCC CACGCAGCAA ACATTGTCAC TGCTATCTAC 
ATCGCATGTG GCCAGGATGC AGCACAGAAT GTGGGGAGTT CAAACTGTAT TACTTTAATG 
GAAGCCAGTG GTCCCACAAA TGAAGACTTA TATATCAGCT GCACCATGCC ATCGATAGAG 
ATAGGAACCG TGGGTGGTGG GACCAACCTT CTACCTCAGC AAGCCTGCCT GCAGATGCTA 
GGTGTTCAAG GAGCATGCAA AGACAATCCT GGAGAAAACG CACGGCAGCT TGCCCGAATT 
GTATGTGGCA CTGTGATGGC TGGTGAGCTG TCCTTGATGG CAGCCTTGGC AGCAGGACAT 
CTTGTCAGAA GTCACATGGT TCACAACAGA TCAAAGATAA ATTTACAAGA TCTTCAAGGA 
ACGTGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmgcr ( NM_001360165.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347094.1 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14982
Clone ID Related Accession (Same CDS sequence)	XM_006517531.1 , NM_008255.2 , NM_001360165.1
Accession Version	NM_001360165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154851.3. On Jan 27, 2018 this sequence version replaced XM_006517531.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.127255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGTCAA GACTTTTCCG GATGCATGGC CTCTTCGTGG CCTCCCACCC CTGGGAAGTT 
ATTGTGGGAA CAGTGACACT TACAATCTGT ATGATGTCCA TGAACATGTT CACCGGCAAC 
AACAAGATCT GTGGCTGGAA TTATGAGTGC CCTAAATTTG AAGAGGACGT GCTGAGCAGC 
GACATCATCA TCCTGACGAT AACGCGGTGC ATCGCCATCC TGTACATTTA CTTCCAGTTC 
CAGAACCTAC GGCAGCTTGG GTCCAAGTAC ATTCTGGGTA TTGCTGGCCT CTTCACAATT 
TTCTCAAGCT TTGTCTTCAG TACAGTCGTC ATTCATTTCC TCGACAAAGA ACTGACAGGC 
TTAAATGAAG CTTTGCCCTT TTTTCTGCTC TTGATTGACC TTTCTAGAGC GAGTGCATTA 
GCAAAGTTTG CCCTCAGTTC AAATTCACAG GATGAAGTAA GGGAAAATAT AGCTCGTGGA 
ATGGCAATCC TGGGCCCCAC ATTCACTCTT GACGCTCTTG TGGAATGCCT TGTGATTGGA 
GTTGGCACCA TGTCAGGCGT CCGCCAGCTG GAGATCATGT GCTGCTTCGG CTGCATGTCA 
GTGTTGGCCA ACTACTTTGT GTTCATGACA TTCTTCCCGG CCTGTGTGTC GCTGGTCCTA 
GAGCTTTCTC GTGAAAGCCG TGAGGGTCGT CCAATTTGGC AGCTCAGCCA TTTTGCCAGA 
GTTTTAGAAG AAGAAGAAAA TAAACCAAAC CCCGTAACCC AAAGGGTCAA GATGATTATG 
TCTTTAGGCT TGGTCCTTGT TCACGCTCAT AGTCGCTGGA TAGCTGATCC TTCTCCTCAG 
AACAGCACAG CGGAGCAGGC TAAGGTTTCC TTGGGTCTGG ACGAAGATGT GTCCAAGAGA 
ATTGAACCAA GTGTTTCTCT CTGGCAGTTT TATCTCTCCA AGATGATCAG CATGGACATT 
GAGCAAGTGA TTACCCTGAG TTTAGCCTTC CTTTTGGCTG TCAAGTATAT TTTCTTTGAA 
CAAGCAGAGA CAGAATCGAC ACTGTCATTA AAAAATCCTA TCACGTCTCC CGTCGTGACC 
TCAAAGAAAG CTCAAGACAA CTGTTGTAGA CGTGAGCCTC TGCTTGTGAG AAGGAACCAG 
AAGCTTTCGT CAGTAGAGGA GGATCCAGGA GCGAACCAAG AGAGAAAAGT TGAGGTTATA 
AAACCATTAG TGGTGGAAGC AGAGACTACA AGCAGAGCTA CGTTTGTGCT TGGGGCTTCT 
GTAGCCAGCC CTCCATCCGC CCTGGGGACA CAGGAGCCTG GAATTGAACT CCCCATCGAG 
CCACGACCTA ATGAAGAATG TCTGCAGATA CTGGAGAATG CAGAGAAAGG TGCAAAGTTC 
CTTAGTGATG CAGAGATCAT CCAGTTGGTC AATGCCAAGC ATATCCCAGC TTACAAATTG 
GAAACTCTAA TGGAAACTCA TGAACGTGGT GTGTCTATTC GCCGGCAGCT CCTCTCCACA 
AAGCTTGCAG AGCCGTCCTC TCTACAGTAC CTGCCTTACA GAGATTATAA TTATTCCTTG 
GTGATGGGAG CTTGCTGTGA GAATGTGATC GGATATATGC CCATCCCTGT TGGAGTGGCA 
GGGCCTCTGT GCCTGGATGG GAAGGAGTAC CAGGTGCCGA TGGCAACGAC GGAAGGCTGT 
CTTGTGGCCA GCACTAACAG AGGCTGCAGA GCCATAAGCC TTGGTGGAGG TGCCAGCAGC 
CGGGTCCTTG CAGATGGGAT GACTCGAGGC CCAGTGGTGC GTCTTCCACG TGCTTGTGAC 
TCTGCAGAAG TAAAGACCTG GCTTGAAACA CCTGAAGGGT TTGCAGTGAT AAAGGAGGCC 
TTTGATAGCA CCAGCAGATT TGCTCGTCTA CAGAAACTCC ACGTGACGAT GGCAGGACGC 
AACCTCTATA TCCGTTTCCA GTCCAGGACG GGGGACGCCA TGGGCATGAA CATGATCTCT 
AAGGGTACGG AGAAAGCACT GCTGAAGCTT CAGGAGTTCT TTCCTGACAT GCAGATTCTG 
GCAGTCAGTG GGAACTATTG CACCGACAAG AAGCCTGCTG CCATAAATTG GATCGAAGGA 
CGAGGAAAGA CTGTGGTTTG TGAAGCCGTC ATTCCAGCCA AGGTGGTGAG AGAGGTGTTA 
AAGACAACTA CGGAAGCTAT GGTTGACGTA AACATTAACA AGAATCTTGT GGGCTCGGCC 
ATGGCTGGGA GCATAGGCGG CTACAACGCC CACGCAGCAA ACATTGTCAC TGCTATCTAC 
ATCGCATGTG GCCAGGATGC AGCACAGAAT GTGGGGAGTT CAAACTGTAT TACTTTAATG 
GAAGCCAGTG GTCCCACAAA TGAAGACTTA TATATCAGCT GCACCATGCC ATCGATAGAG 
ATAGGAACCG TGGGTGGTGG GACCAACCTT CTACCTCAGC AAGCCTGCCT GCAGATGCTA 
GGTGTTCAAG GAGCATGCAA AGACAATCCT GGAGAAAACG CACGGCAGCT TGCCCGAATT 
GTATGTGGCA CTGTGATGGC TGGTGAGCTG TCCTTGATGG CAGCCTTGGC AGCAGGACAT 
CTTGTCAGAA GTCACATGGT TCACAACAGA TCAAAGATAA ATTTACAAGA TCTTCAAGGA 
ACGTGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347094.1
CDS	513..3176
Translation	MLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASALAKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIADPSPQNSTAEQAKVSLGLDEDVSKRIEPSVSLWQFYLSKMISMDIEQVITLSLAFLLAVKYIFFEQAETESTLSLKNPITSPVVTSKKAQDNCCRREPLLVRRNQKLSSVEEDPGANQERKVEVIKPLVVEAETTSRATFVLGASVASPPSALGTQEPGIELPIEPRPNEECLQILENAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSTKLAEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDGKEYQVPMATTEGCLVASTNRGCRAISLGGGASSRVLADGMTRGPVVRLPRACDSAEVKTWLETPEGFAVIKEAFDSTSRFARLQKLHVTMAGRNLYIRFQSRTGDAMGMNMISKGTEKALLKLQEFFPDMQILAVSGNYCTDKKPAAINWIEGRGKTVVCEAVIPAKVVREVLKTTTEAMVDVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVRSHMVHNRSKINLQDLQGTCTKKAA

Target ORF information:

RefSeq Version	NM_001360165.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360165.1

ORF Insert Sequence:

ATGTTGTCAA GACTTTTCCG GATGCATGGC CTCTTCGTGG CCTCCCACCC CTGGGAAGTT 
ATTGTGGGAA CAGTGACACT TACAATCTGT ATGATGTCCA TGAACATGTT CACCGGCAAC 
AACAAGATCT GTGGCTGGAA TTATGAGTGC CCTAAATTTG AAGAGGACGT GCTGAGCAGC 
GACATCATCA TCCTGACGAT AACGCGGTGC ATCGCCATCC TGTACATTTA CTTCCAGTTC 
CAGAACCTAC GGCAGCTTGG GTCCAAGTAC ATTCTGGGTA TTGCTGGCCT CTTCACAATT 
TTCTCAAGCT TTGTCTTCAG TACAGTCGTC ATTCATTTCC TCGACAAAGA ACTGACAGGC 
TTAAATGAAG CTTTGCCCTT TTTTCTGCTC TTGATTGACC TTTCTAGAGC GAGTGCATTA 
GCAAAGTTTG CCCTCAGTTC AAATTCACAG GATGAAGTAA GGGAAAATAT AGCTCGTGGA 
ATGGCAATCC TGGGCCCCAC ATTCACTCTT GACGCTCTTG TGGAATGCCT TGTGATTGGA 
GTTGGCACCA TGTCAGGCGT CCGCCAGCTG GAGATCATGT GCTGCTTCGG CTGCATGTCA 
GTGTTGGCCA ACTACTTTGT GTTCATGACA TTCTTCCCGG CCTGTGTGTC GCTGGTCCTA 
GAGCTTTCTC GTGAAAGCCG TGAGGGTCGT CCAATTTGGC AGCTCAGCCA TTTTGCCAGA 
GTTTTAGAAG AAGAAGAAAA TAAACCAAAC CCCGTAACCC AAAGGGTCAA GATGATTATG 
TCTTTAGGCT TGGTCCTTGT TCACGCTCAT AGTCGCTGGA TAGCTGATCC TTCTCCTCAG 
AACAGCACAG CGGAGCAGGC TAAGGTTTCC TTGGGTCTGG ACGAAGATGT GTCCAAGAGA 
ATTGAACCAA GTGTTTCTCT CTGGCAGTTT TATCTCTCCA AGATGATCAG CATGGACATT 
GAGCAAGTGA TTACCCTGAG TTTAGCCTTC CTTTTGGCTG TCAAGTATAT TTTCTTTGAA 
CAAGCAGAGA CAGAATCGAC ACTGTCATTA AAAAATCCTA TCACGTCTCC CGTCGTGACC 
TCAAAGAAAG CTCAAGACAA CTGTTGTAGA CGTGAGCCTC TGCTTGTGAG AAGGAACCAG 
AAGCTTTCGT CAGTAGAGGA GGATCCAGGA GCGAACCAAG AGAGAAAAGT TGAGGTTATA 
AAACCATTAG TGGTGGAAGC AGAGACTACA AGCAGAGCTA CGTTTGTGCT TGGGGCTTCT 
GTAGCCAGCC CTCCATCCGC CCTGGGGACA CAGGAGCCTG GAATTGAACT CCCCATCGAG 
CCACGACCTA ATGAAGAATG TCTGCAGATA CTGGAGAATG CAGAGAAAGG TGCAAAGTTC 
CTTAGTGATG CAGAGATCAT CCAGTTGGTC AATGCCAAGC ATATCCCAGC TTACAAATTG 
GAAACTCTAA TGGAAACTCA TGAACGTGGT GTGTCTATTC GCCGGCAGCT CCTCTCCACA 
AAGCTTGCAG AGCCGTCCTC TCTACAGTAC CTGCCTTACA GAGATTATAA TTATTCCTTG 
GTGATGGGAG CTTGCTGTGA GAATGTGATC GGATATATGC CCATCCCTGT TGGAGTGGCA 
GGGCCTCTGT GCCTGGATGG GAAGGAGTAC CAGGTGCCGA TGGCAACGAC GGAAGGCTGT 
CTTGTGGCCA GCACTAACAG AGGCTGCAGA GCCATAAGCC TTGGTGGAGG TGCCAGCAGC 
CGGGTCCTTG CAGATGGGAT GACTCGAGGC CCAGTGGTGC GTCTTCCACG TGCTTGTGAC 
TCTGCAGAAG TAAAGACCTG GCTTGAAACA CCTGAAGGGT TTGCAGTGAT AAAGGAGGCC 
TTTGATAGCA CCAGCAGATT TGCTCGTCTA CAGAAACTCC ACGTGACGAT GGCAGGACGC 
AACCTCTATA TCCGTTTCCA GTCCAGGACG GGGGACGCCA TGGGCATGAA CATGATCTCT 
AAGGGTACGG AGAAAGCACT GCTGAAGCTT CAGGAGTTCT TTCCTGACAT GCAGATTCTG 
GCAGTCAGTG GGAACTATTG CACCGACAAG AAGCCTGCTG CCATAAATTG GATCGAAGGA 
CGAGGAAAGA CTGTGGTTTG TGAAGCCGTC ATTCCAGCCA AGGTGGTGAG AGAGGTGTTA 
AAGACAACTA CGGAAGCTAT GGTTGACGTA AACATTAACA AGAATCTTGT GGGCTCGGCC 
ATGGCTGGGA GCATAGGCGG CTACAACGCC CACGCAGCAA ACATTGTCAC TGCTATCTAC 
ATCGCATGTG GCCAGGATGC AGCACAGAAT GTGGGGAGTT CAAACTGTAT TACTTTAATG 
GAAGCCAGTG GTCCCACAAA TGAAGACTTA TATATCAGCT GCACCATGCC ATCGATAGAG 
ATAGGAACCG TGGGTGGTGG GACCAACCTT CTACCTCAGC AAGCCTGCCT GCAGATGCTA 
GGTGTTCAAG GAGCATGCAA AGACAATCCT GGAGAAAACG CACGGCAGCT TGCCCGAATT 
GTATGTGGCA CTGTGATGGC TGGTGAGCTG TCCTTGATGG CAGCCTTGGC AGCAGGACAT 
CTTGTCAGAA GTCACATGGT TCACAACAGA TCAAAGATAA ATTTACAAGA TCTTCAAGGA 
ACGTGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmgcr ( NM_001360166.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347095.1 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant 3, mRNA.

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CloneID	OMu48955
Clone ID Related Accession (Same CDS sequence)	NM_001360166.1 , XM_006517530.1
Accession Version	NM_001360166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154851.3. On Jan 27, 2018 this sequence version replaced XM_006517530.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.76641.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGAGGAT CCAAGGACTG TGAGGCTACA ATGTTGTCAA GACTTTTCCG GATGCATGGC 
CTCTTCGTGG CCTCCCACCC CTGGGAAGTT ATTGTGGGAA CAGTGACACT TACAATCTGT 
ATGATGTCCA TGAACATGTT CACCGGCAAC AACAAGATCT GTGGCTGGAA TTATGAGTGC 
CCTAAATTTG AAGAGGACGT GCTGAGCAGC GACATCATCA TCCTGACGAT AACGCGGTGC 
ATCGCCATCC TGTACATTTA CTTCCAGTTC CAGAACCTAC GGCAGCTTGG GTCCAAGTAC 
ATTCTGGGTA TTGCTGGCCT CTTCACAATT TTCTCAAGCT TTGTCTTCAG TACAGTCGTC 
ATTCATTTCC TCGACAAAGA ACTGACAGGC TTAAATGAAG CTTTGCCCTT TTTTCTGCTC 
TTGATTGACC TTTCTAGAGC GAGTGCATTA GCAAAGTTTG CCCTCAGTTC AAATTCACAG 
GATGAAGTAA GGGAAAATAT AGCTCGTGGA ATGGCAATCC TGGGCCCCAC ATTCACTCTT 
GACGCTCTTG TGGAATGCCT TGTGATTGGA GTTGGCACCA TGTCAGGCGT CCGCCAGCTG 
GAGATCATGT GCTGCTTCGG CTGCATGTCA GTGTTGGCCA ACTACTTTGT GTTCATGACA 
TTCTTCCCGG CCTGTGTGTC GCTGGTCCTA GAGCTTTCTC GTGAAAGCCG TGAGGGTCGT 
CCAATTTGGC AGCTCAGCCA TTTTGCCAGA GTTTTAGAAG AAGAAGAAAA TAAACCAAAC 
CCCGTAACCC AAAGGGTCAA GATGATTATG TCTTTAGGCT TGGTCCTTGT TCACGCTCAT 
AGTCGCTGGA TAGCTGATCC TTCTCCTCAG AACAGCACAG CGGAGCAGGC TAAGGTTTCC 
TTGGGTCTGG ACGAAGATGT GTCCAAGAGA ATTGAACCAA GTGTTTCTCT CTGGCAGTTT 
TATCTCTCCA AGATGATCAG CATGGACATT GAGCAAGTGA TTACCCTGAG TTTAGCCTTC 
CTTTTGGCTG TCAAGTATAT TTTCTTTGAA CAAGCAGAGA CAGAATCGAC ACTGTCATTA 
AAAAATCCTA TCACGTCTCC CGTCGTGACC TCAAAGAAAG CTCAAGACAA CTGTTGTAGA 
CGTGAGCCTC TGCTTGTGAG AAGGAACCAG AAGCTTTCGT CAGTAGAGGA GGATCCAGGA 
GCGAACCAAG AGAGAAAAGT TGAGGTTATA AAACCATTAG TGGTGGAAGC AGAGACTACA 
AGCAGAGCTA CGTTTGTGCT TGGGGCTTCT GTAGCCAGCC CTCCATCCGC CCTGGGGACA 
CAGGAGCCTG GAATTGAACT CCCCATCGAG CCACGACCTA ATGAAGAATG TCTGCAGATA 
CTGGAGAATG CAGAGAAAGG TGCAAAGTTC CTTAGTGATG CAGAGATCAT CCAGTTGGTC 
AATGCCAAGC ATATCCCAGC TTACAAATTG GAAACTCTAA TGGAAACTCA TGAACGTGGT 
GTGTCTATTC GCCGGCAGCT CCTCTCCACA AAGCTTGCAG AGCCGTCCTC TCTACAGTAC 
CTGCCTTACA GAGATTATAA TTATTCCTTG GTGATGGGAG CTTGCTGTGA GAATGTGATC 
GGATATATGC CCATCCCTGT TGGAGTGGCA GGGCCTCTGT GCCTGGATGG GAAGGAGTAC 
CAGGTGCCGA TGGCAACGAC GGAAGGCTGT CTTGTGGCCA GCACTAACAG AGGCTGCAGA 
GCCATAAGCC TTGGTGGAGG TGCCAGCAGC CGGGTCCTTG CAGATGGGAT GACTCGAGGC 
CCAGTGGTGC GTCTTCCACG TGCTTGTGAC TCTGCAGAAG TAAAGACCTG GCTTGAAACA 
CCTGAAGGGT TTGCAGTGAT AAAGGAGGCC TTTGATAGCA CCAGCAGATT TGCTCGTCTA 
CAGAAACTCC ACGTGACGAT GGCAGGACGC AACCTCTATA TCCGTTTCCA GTCCAGGACG 
GGGGACGCCA TGGGCATGAA CATGATCTCT AAGGGTACGG AGAAAGCACT GCTGAAGCTT 
CAGGAGTTCT TTCCTGACAT GCAGATTCTG GCAGTCAGTG GGAACTATTG CACCGACAAG 
AAGCCTGCTG CCATAAATTG GATCGAAGGA CGAGGAAAGA CTGTGGTTTG TGAAGCCGTC 
ATTCCAGCCA AGGTGGTGAG AGAGGTGTTA AAGACAACTA CGGAAGCTAT GGTTGACGTA 
AACATTAACA AGAATCTTGT GGGCTCGGCC ATGGCTGGGA GCATAGGCGG CTACAACGCC 
CACGCAGCAA ACATTGTCAC TGCTATCTAC ATCGCATGTG GCCAGGATGC AGCACAGAAT 
GTGGGGAGTT CAAACTGTAT TACTTTAATG GAAGCCAGTG GTCCCACAAA TGAAGACTTA 
TATATCAGCT GCACCATGCC ATCGATAGAG ATAGGAACCG TGGGTGGTGG GACCAACCTT 
CTACCTCAGC AAGCCTGCCT GCAGATGCTA GGTGTTCAAG GAGCATGCAA AGACAATCCT 
GGAGAAAACG CACGGCAGCT TGCCCGAATT GTATGTGGCA CTGTGATGGC TGGTGAGCTG 
TCCTTGATGG CAGCCTTGGC AGCAGGACAT CTTGTCAGAA GTCACATGGT TCACAACAGA 
TCAAAGATAA ATTTACAAGA TCTTCAAGGA ACGTGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347095.1
CDS	530..3223
Translation	MRGSKDCEATMLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASALAKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIADPSPQNSTAEQAKVSLGLDEDVSKRIEPSVSLWQFYLSKMISMDIEQVITLSLAFLLAVKYIFFEQAETESTLSLKNPITSPVVTSKKAQDNCCRREPLLVRRNQKLSSVEEDPGANQERKVEVIKPLVVEAETTSRATFVLGASVASPPSALGTQEPGIELPIEPRPNEECLQILENAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSTKLAEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDGKEYQVPMATTEGCLVASTNRGCRAISLGGGASSRVLADGMTRGPVVRLPRACDSAEVKTWLETPEGFAVIKEAFDSTSRFARLQKLHVTMAGRNLYIRFQSRTGDAMGMNMISKGTEKALLKLQEFFPDMQILAVSGNYCTDKKPAAINWIEGRGKTVVCEAVIPAKVVREVLKTTTEAMVDVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVRSHMVHNRSKINLQDLQGTCTKKAA

Target ORF information:

RefSeq Version	NM_001360166.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360166.1

ORF Insert Sequence:

ATGCGAGGAT CCAAGGACTG TGAGGCTACA ATGTTGTCAA GACTTTTCCG GATGCATGGC 
CTCTTCGTGG CCTCCCACCC CTGGGAAGTT ATTGTGGGAA CAGTGACACT TACAATCTGT 
ATGATGTCCA TGAACATGTT CACCGGCAAC AACAAGATCT GTGGCTGGAA TTATGAGTGC 
CCTAAATTTG AAGAGGACGT GCTGAGCAGC GACATCATCA TCCTGACGAT AACGCGGTGC 
ATCGCCATCC TGTACATTTA CTTCCAGTTC CAGAACCTAC GGCAGCTTGG GTCCAAGTAC 
ATTCTGGGTA TTGCTGGCCT CTTCACAATT TTCTCAAGCT TTGTCTTCAG TACAGTCGTC 
ATTCATTTCC TCGACAAAGA ACTGACAGGC TTAAATGAAG CTTTGCCCTT TTTTCTGCTC 
TTGATTGACC TTTCTAGAGC GAGTGCATTA GCAAAGTTTG CCCTCAGTTC AAATTCACAG 
GATGAAGTAA GGGAAAATAT AGCTCGTGGA ATGGCAATCC TGGGCCCCAC ATTCACTCTT 
GACGCTCTTG TGGAATGCCT TGTGATTGGA GTTGGCACCA TGTCAGGCGT CCGCCAGCTG 
GAGATCATGT GCTGCTTCGG CTGCATGTCA GTGTTGGCCA ACTACTTTGT GTTCATGACA 
TTCTTCCCGG CCTGTGTGTC GCTGGTCCTA GAGCTTTCTC GTGAAAGCCG TGAGGGTCGT 
CCAATTTGGC AGCTCAGCCA TTTTGCCAGA GTTTTAGAAG AAGAAGAAAA TAAACCAAAC 
CCCGTAACCC AAAGGGTCAA GATGATTATG TCTTTAGGCT TGGTCCTTGT TCACGCTCAT 
AGTCGCTGGA TAGCTGATCC TTCTCCTCAG AACAGCACAG CGGAGCAGGC TAAGGTTTCC 
TTGGGTCTGG ACGAAGATGT GTCCAAGAGA ATTGAACCAA GTGTTTCTCT CTGGCAGTTT 
TATCTCTCCA AGATGATCAG CATGGACATT GAGCAAGTGA TTACCCTGAG TTTAGCCTTC 
CTTTTGGCTG TCAAGTATAT TTTCTTTGAA CAAGCAGAGA CAGAATCGAC ACTGTCATTA 
AAAAATCCTA TCACGTCTCC CGTCGTGACC TCAAAGAAAG CTCAAGACAA CTGTTGTAGA 
CGTGAGCCTC TGCTTGTGAG AAGGAACCAG AAGCTTTCGT CAGTAGAGGA GGATCCAGGA 
GCGAACCAAG AGAGAAAAGT TGAGGTTATA AAACCATTAG TGGTGGAAGC AGAGACTACA 
AGCAGAGCTA CGTTTGTGCT TGGGGCTTCT GTAGCCAGCC CTCCATCCGC CCTGGGGACA 
CAGGAGCCTG GAATTGAACT CCCCATCGAG CCACGACCTA ATGAAGAATG TCTGCAGATA 
CTGGAGAATG CAGAGAAAGG TGCAAAGTTC CTTAGTGATG CAGAGATCAT CCAGTTGGTC 
AATGCCAAGC ATATCCCAGC TTACAAATTG GAAACTCTAA TGGAAACTCA TGAACGTGGT 
GTGTCTATTC GCCGGCAGCT CCTCTCCACA AAGCTTGCAG AGCCGTCCTC TCTACAGTAC 
CTGCCTTACA GAGATTATAA TTATTCCTTG GTGATGGGAG CTTGCTGTGA GAATGTGATC 
GGATATATGC CCATCCCTGT TGGAGTGGCA GGGCCTCTGT GCCTGGATGG GAAGGAGTAC 
CAGGTGCCGA TGGCAACGAC GGAAGGCTGT CTTGTGGCCA GCACTAACAG AGGCTGCAGA 
GCCATAAGCC TTGGTGGAGG TGCCAGCAGC CGGGTCCTTG CAGATGGGAT GACTCGAGGC 
CCAGTGGTGC GTCTTCCACG TGCTTGTGAC TCTGCAGAAG TAAAGACCTG GCTTGAAACA 
CCTGAAGGGT TTGCAGTGAT AAAGGAGGCC TTTGATAGCA CCAGCAGATT TGCTCGTCTA 
CAGAAACTCC ACGTGACGAT GGCAGGACGC AACCTCTATA TCCGTTTCCA GTCCAGGACG 
GGGGACGCCA TGGGCATGAA CATGATCTCT AAGGGTACGG AGAAAGCACT GCTGAAGCTT 
CAGGAGTTCT TTCCTGACAT GCAGATTCTG GCAGTCAGTG GGAACTATTG CACCGACAAG 
AAGCCTGCTG CCATAAATTG GATCGAAGGA CGAGGAAAGA CTGTGGTTTG TGAAGCCGTC 
ATTCCAGCCA AGGTGGTGAG AGAGGTGTTA AAGACAACTA CGGAAGCTAT GGTTGACGTA 
AACATTAACA AGAATCTTGT GGGCTCGGCC ATGGCTGGGA GCATAGGCGG CTACAACGCC 
CACGCAGCAA ACATTGTCAC TGCTATCTAC ATCGCATGTG GCCAGGATGC AGCACAGAAT 
GTGGGGAGTT CAAACTGTAT TACTTTAATG GAAGCCAGTG GTCCCACAAA TGAAGACTTA 
TATATCAGCT GCACCATGCC ATCGATAGAG ATAGGAACCG TGGGTGGTGG GACCAACCTT 
CTACCTCAGC AAGCCTGCCT GCAGATGCTA GGTGTTCAAG GAGCATGCAA AGACAATCCT 
GGAGAAAACG CACGGCAGCT TGCCCGAATT GTATGTGGCA CTGTGATGGC TGGTGAGCTG 
TCCTTGATGG CAGCCTTGGC AGCAGGACAT CTTGTCAGAA GTCACATGGT TCACAACAGA 
TCAAAGATAA ATTTACAAGA TCTTCAAGGA ACGTGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmgcr ( XM_006517531.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517594.1 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu14982
Clone ID Related Accession (Same CDS sequence)	XM_006517531.1 , NM_008255.2 , NM_001360165.1
Accession Version	XM_006517531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAA GACTTTTCCG GATGCATGGC CTCTTCGTGG CCTCCCACCC CTGGGAAGTT 
ATTGTGGGAA CAGTGACACT TACAATCTGT ATGATGTCCA TGAACATGTT CACCGGCAAC 
AACAAGATCT GTGGCTGGAA TTATGAGTGC CCTAAATTTG AAGAGGACGT GCTGAGCAGC 
GACATCATCA TCCTGACGAT AACGCGGTGC ATCGCCATCC TGTACATTTA CTTCCAGTTC 
CAGAACCTAC GGCAGCTTGG GTCCAAGTAC ATTCTGGGTA TTGCTGGCCT CTTCACAATT 
TTCTCAAGCT TTGTCTTCAG TACAGTCGTC ATTCATTTCC TCGACAAAGA ACTGACAGGC 
TTAAATGAAG CTTTGCCCTT TTTTCTGCTC TTGATTGACC TTTCTAGAGC GAGTGCATTA 
GCAAAGTTTG CCCTCAGTTC AAATTCACAG GATGAAGTAA GGGAAAATAT AGCTCGTGGA 
ATGGCAATCC TGGGCCCCAC ATTCACTCTT GACGCTCTTG TGGAATGCCT TGTGATTGGA 
GTTGGCACCA TGTCAGGCGT CCGCCAGCTG GAGATCATGT GCTGCTTCGG CTGCATGTCA 
GTGTTGGCCA ACTACTTTGT GTTCATGACA TTCTTCCCGG CCTGTGTGTC GCTGGTCCTA 
GAGCTTTCTC GTGAAAGCCG TGAGGGTCGT CCAATTTGGC AGCTCAGCCA TTTTGCCAGA 
GTTTTAGAAG AAGAAGAAAA TAAACCAAAC CCCGTAACCC AAAGGGTCAA GATGATTATG 
TCTTTAGGCT TGGTCCTTGT TCACGCTCAT AGTCGCTGGA TAGCTGATCC TTCTCCTCAG 
AACAGCACAG CGGAGCAGGC TAAGGTTTCC TTGGGTCTGG ACGAAGATGT GTCCAAGAGA 
ATTGAACCAA GTGTTTCTCT CTGGCAGTTT TATCTCTCCA AGATGATCAG CATGGACATT 
GAGCAAGTGA TTACCCTGAG TTTAGCCTTC CTTTTGGCTG TCAAGTATAT TTTCTTTGAA 
CAAGCAGAGA CAGAATCGAC ACTGTCATTA AAAAATCCTA TCACGTCTCC CGTCGTGACC 
TCAAAGAAAG CTCAAGACAA CTGTTGTAGA CGTGAGCCTC TGCTTGTGAG AAGGAACCAG 
AAGCTTTCGT CAGTAGAGGA GGATCCAGGA GCGAACCAAG AGAGAAAAGT TGAGGTTATA 
AAACCATTAG TGGTGGAAGC AGAGACTACA AGCAGAGCTA CGTTTGTGCT TGGGGCTTCT 
GTAGCCAGCC CTCCATCCGC CCTGGGGACA CAGGAGCCTG GAATTGAACT CCCCATCGAG 
CCACGACCTA ATGAAGAATG TCTGCAGATA CTGGAGAATG CAGAGAAAGG TGCAAAGTTC 
CTTAGTGATG CAGAGATCAT CCAGTTGGTC AATGCCAAGC ATATCCCAGC TTACAAATTG 
GAAACTCTAA TGGAAACTCA TGAACGTGGT GTGTCTATTC GCCGGCAGCT CCTCTCCACA 
AAGCTTGCAG AGCCGTCCTC TCTACAGTAC CTGCCTTACA GAGATTATAA TTATTCCTTG 
GTGATGGGAG CTTGCTGTGA GAATGTGATC GGATATATGC CCATCCCTGT TGGAGTGGCA 
GGGCCTCTGT GCCTGGATGG GAAGGAGTAC CAGGTGCCGA TGGCAACGAC GGAAGGCTGT 
CTTGTGGCCA GCACTAACAG AGGCTGCAGA GCCATAAGCC TTGGTGGAGG TGCCAGCAGC 
CGGGTCCTTG CAGATGGGAT GACTCGAGGC CCAGTGGTGC GTCTTCCACG TGCTTGTGAC 
TCTGCAGAAG TAAAGACCTG GCTTGAAACA CCTGAAGGGT TTGCAGTGAT AAAGGAGGCC 
TTTGATAGCA CCAGCAGATT TGCTCGTCTA CAGAAACTCC ACGTGACGAT GGCAGGACGC 
AACCTCTATA TCCGTTTCCA GTCCAGGACG GGGGACGCCA TGGGCATGAA CATGATCTCT 
AAGGGTACGG AGAAAGCACT GCTGAAGCTT CAGGAGTTCT TTCCTGACAT GCAGATTCTG 
GCAGTCAGTG GGAACTATTG CACCGACAAG AAGCCTGCTG CCATAAATTG GATCGAAGGA 
CGAGGAAAGA CTGTGGTTTG TGAAGCCGTC ATTCCAGCCA AGGTGGTGAG AGAGGTGTTA 
AAGACAACTA CGGAAGCTAT GGTTGACGTA AACATTAACA AGAATCTTGT GGGCTCGGCC 
ATGGCTGGGA GCATAGGCGG CTACAACGCC CACGCAGCAA ACATTGTCAC TGCTATCTAC 
ATCGCATGTG GCCAGGATGC AGCACAGAAT GTGGGGAGTT CAAACTGTAT TACTTTAATG 
GAAGCCAGTG GTCCCACAAA TGAAGACTTA TATATCAGCT GCACCATGCC ATCGATAGAG 
ATAGGAACCG TGGGTGGTGG GACCAACCTT CTACCTCAGC AAGCCTGCCT GCAGATGCTA 
GGTGTTCAAG GAGCATGCAA AGACAATCCT GGAGAAAACG CACGGCAGCT TGCCCGAATT 
GTATGTGGCA CTGTGATGGC TGGTGAGCTG TCCTTGATGG CAGCCTTGGC AGCAGGACAT 
CTTGTCAGAA GTCACATGGT TCACAACAGA TCAAAGATAA ATTTACAAGA TCTTCAAGGA 
ACGTGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517594.1
CDS	400..3063
Translation	MLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASALAKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIADPSPQNSTAEQAKVSLGLDEDVSKRIEPSVSLWQFYLSKMISMDIEQVITLSLAFLLAVKYIFFEQAETESTLSLKNPITSPVVTSKKAQDNCCRREPLLVRRNQKLSSVEEDPGANQERKVEVIKPLVVEAETTSRATFVLGASVASPPSALGTQEPGIELPIEPRPNEECLQILENAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSTKLAEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDGKEYQVPMATTEGCLVASTNRGCRAISLGGGASSRVLADGMTRGPVVRLPRACDSAEVKTWLETPEGFAVIKEAFDSTSRFARLQKLHVTMAGRNLYIRFQSRTGDAMGMNMISKGTEKALLKLQEFFPDMQILAVSGNYCTDKKPAAINWIEGRGKTVVCEAVIPAKVVREVLKTTTEAMVDVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVRSHMVHNRSKINLQDLQGTCTKKAA

Target ORF information:

RefSeq Version	XM_006517531.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517531.1

ORF Insert Sequence:

ATGTTGTCAA GACTTTTCCG GATGCATGGC CTCTTCGTGG CCTCCCACCC CTGGGAAGTT 
ATTGTGGGAA CAGTGACACT TACAATCTGT ATGATGTCCA TGAACATGTT CACCGGCAAC 
AACAAGATCT GTGGCTGGAA TTATGAGTGC CCTAAATTTG AAGAGGACGT GCTGAGCAGC 
GACATCATCA TCCTGACGAT AACGCGGTGC ATCGCCATCC TGTACATTTA CTTCCAGTTC 
CAGAACCTAC GGCAGCTTGG GTCCAAGTAC ATTCTGGGTA TTGCTGGCCT CTTCACAATT 
TTCTCAAGCT TTGTCTTCAG TACAGTCGTC ATTCATTTCC TCGACAAAGA ACTGACAGGC 
TTAAATGAAG CTTTGCCCTT TTTTCTGCTC TTGATTGACC TTTCTAGAGC GAGTGCATTA 
GCAAAGTTTG CCCTCAGTTC AAATTCACAG GATGAAGTAA GGGAAAATAT AGCTCGTGGA 
ATGGCAATCC TGGGCCCCAC ATTCACTCTT GACGCTCTTG TGGAATGCCT TGTGATTGGA 
GTTGGCACCA TGTCAGGCGT CCGCCAGCTG GAGATCATGT GCTGCTTCGG CTGCATGTCA 
GTGTTGGCCA ACTACTTTGT GTTCATGACA TTCTTCCCGG CCTGTGTGTC GCTGGTCCTA 
GAGCTTTCTC GTGAAAGCCG TGAGGGTCGT CCAATTTGGC AGCTCAGCCA TTTTGCCAGA 
GTTTTAGAAG AAGAAGAAAA TAAACCAAAC CCCGTAACCC AAAGGGTCAA GATGATTATG 
TCTTTAGGCT TGGTCCTTGT TCACGCTCAT AGTCGCTGGA TAGCTGATCC TTCTCCTCAG 
AACAGCACAG CGGAGCAGGC TAAGGTTTCC TTGGGTCTGG ACGAAGATGT GTCCAAGAGA 
ATTGAACCAA GTGTTTCTCT CTGGCAGTTT TATCTCTCCA AGATGATCAG CATGGACATT 
GAGCAAGTGA TTACCCTGAG TTTAGCCTTC CTTTTGGCTG TCAAGTATAT TTTCTTTGAA 
CAAGCAGAGA CAGAATCGAC ACTGTCATTA AAAAATCCTA TCACGTCTCC CGTCGTGACC 
TCAAAGAAAG CTCAAGACAA CTGTTGTAGA CGTGAGCCTC TGCTTGTGAG AAGGAACCAG 
AAGCTTTCGT CAGTAGAGGA GGATCCAGGA GCGAACCAAG AGAGAAAAGT TGAGGTTATA 
AAACCATTAG TGGTGGAAGC AGAGACTACA AGCAGAGCTA CGTTTGTGCT TGGGGCTTCT 
GTAGCCAGCC CTCCATCCGC CCTGGGGACA CAGGAGCCTG GAATTGAACT CCCCATCGAG 
CCACGACCTA ATGAAGAATG TCTGCAGATA CTGGAGAATG CAGAGAAAGG TGCAAAGTTC 
CTTAGTGATG CAGAGATCAT CCAGTTGGTC AATGCCAAGC ATATCCCAGC TTACAAATTG 
GAAACTCTAA TGGAAACTCA TGAACGTGGT GTGTCTATTC GCCGGCAGCT CCTCTCCACA 
AAGCTTGCAG AGCCGTCCTC TCTACAGTAC CTGCCTTACA GAGATTATAA TTATTCCTTG 
GTGATGGGAG CTTGCTGTGA GAATGTGATC GGATATATGC CCATCCCTGT TGGAGTGGCA 
GGGCCTCTGT GCCTGGATGG GAAGGAGTAC CAGGTGCCGA TGGCAACGAC GGAAGGCTGT 
CTTGTGGCCA GCACTAACAG AGGCTGCAGA GCCATAAGCC TTGGTGGAGG TGCCAGCAGC 
CGGGTCCTTG CAGATGGGAT GACTCGAGGC CCAGTGGTGC GTCTTCCACG TGCTTGTGAC 
TCTGCAGAAG TAAAGACCTG GCTTGAAACA CCTGAAGGGT TTGCAGTGAT AAAGGAGGCC 
TTTGATAGCA CCAGCAGATT TGCTCGTCTA CAGAAACTCC ACGTGACGAT GGCAGGACGC 
AACCTCTATA TCCGTTTCCA GTCCAGGACG GGGGACGCCA TGGGCATGAA CATGATCTCT 
AAGGGTACGG AGAAAGCACT GCTGAAGCTT CAGGAGTTCT TTCCTGACAT GCAGATTCTG 
GCAGTCAGTG GGAACTATTG CACCGACAAG AAGCCTGCTG CCATAAATTG GATCGAAGGA 
CGAGGAAAGA CTGTGGTTTG TGAAGCCGTC ATTCCAGCCA AGGTGGTGAG AGAGGTGTTA 
AAGACAACTA CGGAAGCTAT GGTTGACGTA AACATTAACA AGAATCTTGT GGGCTCGGCC 
ATGGCTGGGA GCATAGGCGG CTACAACGCC CACGCAGCAA ACATTGTCAC TGCTATCTAC 
ATCGCATGTG GCCAGGATGC AGCACAGAAT GTGGGGAGTT CAAACTGTAT TACTTTAATG 
GAAGCCAGTG GTCCCACAAA TGAAGACTTA TATATCAGCT GCACCATGCC ATCGATAGAG 
ATAGGAACCG TGGGTGGTGG GACCAACCTT CTACCTCAGC AAGCCTGCCT GCAGATGCTA 
GGTGTTCAAG GAGCATGCAA AGACAATCCT GGAGAAAACG CACGGCAGCT TGCCCGAATT 
GTATGTGGCA CTGTGATGGC TGGTGAGCTG TCCTTGATGG CAGCCTTGGC AGCAGGACAT 
CTTGTCAGAA GTCACATGGT TCACAACAGA TCAAAGATAA ATTTACAAGA TCTTCAAGGA 
ACGTGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmgcr ( XM_006517530.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517593.1 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant X1, mRNA.

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CloneID	OMu48955
Clone ID Related Accession (Same CDS sequence)	NM_001360166.1 , XM_006517530.1
Accession Version	XM_006517530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGAT CCAAGGACTG TGAGGCTACA ATGTTGTCAA GACTTTTCCG GATGCATGGC 
CTCTTCGTGG CCTCCCACCC CTGGGAAGTT ATTGTGGGAA CAGTGACACT TACAATCTGT 
ATGATGTCCA TGAACATGTT CACCGGCAAC AACAAGATCT GTGGCTGGAA TTATGAGTGC 
CCTAAATTTG AAGAGGACGT GCTGAGCAGC GACATCATCA TCCTGACGAT AACGCGGTGC 
ATCGCCATCC TGTACATTTA CTTCCAGTTC CAGAACCTAC GGCAGCTTGG GTCCAAGTAC 
ATTCTGGGTA TTGCTGGCCT CTTCACAATT TTCTCAAGCT TTGTCTTCAG TACAGTCGTC 
ATTCATTTCC TCGACAAAGA ACTGACAGGC TTAAATGAAG CTTTGCCCTT TTTTCTGCTC 
TTGATTGACC TTTCTAGAGC GAGTGCATTA GCAAAGTTTG CCCTCAGTTC AAATTCACAG 
GATGAAGTAA GGGAAAATAT AGCTCGTGGA ATGGCAATCC TGGGCCCCAC ATTCACTCTT 
GACGCTCTTG TGGAATGCCT TGTGATTGGA GTTGGCACCA TGTCAGGCGT CCGCCAGCTG 
GAGATCATGT GCTGCTTCGG CTGCATGTCA GTGTTGGCCA ACTACTTTGT GTTCATGACA 
TTCTTCCCGG CCTGTGTGTC GCTGGTCCTA GAGCTTTCTC GTGAAAGCCG TGAGGGTCGT 
CCAATTTGGC AGCTCAGCCA TTTTGCCAGA GTTTTAGAAG AAGAAGAAAA TAAACCAAAC 
CCCGTAACCC AAAGGGTCAA GATGATTATG TCTTTAGGCT TGGTCCTTGT TCACGCTCAT 
AGTCGCTGGA TAGCTGATCC TTCTCCTCAG AACAGCACAG CGGAGCAGGC TAAGGTTTCC 
TTGGGTCTGG ACGAAGATGT GTCCAAGAGA ATTGAACCAA GTGTTTCTCT CTGGCAGTTT 
TATCTCTCCA AGATGATCAG CATGGACATT GAGCAAGTGA TTACCCTGAG TTTAGCCTTC 
CTTTTGGCTG TCAAGTATAT TTTCTTTGAA CAAGCAGAGA CAGAATCGAC ACTGTCATTA 
AAAAATCCTA TCACGTCTCC CGTCGTGACC TCAAAGAAAG CTCAAGACAA CTGTTGTAGA 
CGTGAGCCTC TGCTTGTGAG AAGGAACCAG AAGCTTTCGT CAGTAGAGGA GGATCCAGGA 
GCGAACCAAG AGAGAAAAGT TGAGGTTATA AAACCATTAG TGGTGGAAGC AGAGACTACA 
AGCAGAGCTA CGTTTGTGCT TGGGGCTTCT GTAGCCAGCC CTCCATCCGC CCTGGGGACA 
CAGGAGCCTG GAATTGAACT CCCCATCGAG CCACGACCTA ATGAAGAATG TCTGCAGATA 
CTGGAGAATG CAGAGAAAGG TGCAAAGTTC CTTAGTGATG CAGAGATCAT CCAGTTGGTC 
AATGCCAAGC ATATCCCAGC TTACAAATTG GAAACTCTAA TGGAAACTCA TGAACGTGGT 
GTGTCTATTC GCCGGCAGCT CCTCTCCACA AAGCTTGCAG AGCCGTCCTC TCTACAGTAC 
CTGCCTTACA GAGATTATAA TTATTCCTTG GTGATGGGAG CTTGCTGTGA GAATGTGATC 
GGATATATGC CCATCCCTGT TGGAGTGGCA GGGCCTCTGT GCCTGGATGG GAAGGAGTAC 
CAGGTGCCGA TGGCAACGAC GGAAGGCTGT CTTGTGGCCA GCACTAACAG AGGCTGCAGA 
GCCATAAGCC TTGGTGGAGG TGCCAGCAGC CGGGTCCTTG CAGATGGGAT GACTCGAGGC 
CCAGTGGTGC GTCTTCCACG TGCTTGTGAC TCTGCAGAAG TAAAGACCTG GCTTGAAACA 
CCTGAAGGGT TTGCAGTGAT AAAGGAGGCC TTTGATAGCA CCAGCAGATT TGCTCGTCTA 
CAGAAACTCC ACGTGACGAT GGCAGGACGC AACCTCTATA TCCGTTTCCA GTCCAGGACG 
GGGGACGCCA TGGGCATGAA CATGATCTCT AAGGGTACGG AGAAAGCACT GCTGAAGCTT 
CAGGAGTTCT TTCCTGACAT GCAGATTCTG GCAGTCAGTG GGAACTATTG CACCGACAAG 
AAGCCTGCTG CCATAAATTG GATCGAAGGA CGAGGAAAGA CTGTGGTTTG TGAAGCCGTC 
ATTCCAGCCA AGGTGGTGAG AGAGGTGTTA AAGACAACTA CGGAAGCTAT GGTTGACGTA 
AACATTAACA AGAATCTTGT GGGCTCGGCC ATGGCTGGGA GCATAGGCGG CTACAACGCC 
CACGCAGCAA ACATTGTCAC TGCTATCTAC ATCGCATGTG GCCAGGATGC AGCACAGAAT 
GTGGGGAGTT CAAACTGTAT TACTTTAATG GAAGCCAGTG GTCCCACAAA TGAAGACTTA 
TATATCAGCT GCACCATGCC ATCGATAGAG ATAGGAACCG TGGGTGGTGG GACCAACCTT 
CTACCTCAGC AAGCCTGCCT GCAGATGCTA GGTGTTCAAG GAGCATGCAA AGACAATCCT 
GGAGAAAACG CACGGCAGCT TGCCCGAATT GTATGTGGCA CTGTGATGGC TGGTGAGCTG 
TCCTTGATGG CAGCCTTGGC AGCAGGACAT CTTGTCAGAA GTCACATGGT TCACAACAGA 
TCAAAGATAA ATTTACAAGA TCTTCAAGGA ACGTGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517593.1
CDS	398..3091
Translation	MRGSKDCEATMLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASALAKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIADPSPQNSTAEQAKVSLGLDEDVSKRIEPSVSLWQFYLSKMISMDIEQVITLSLAFLLAVKYIFFEQAETESTLSLKNPITSPVVTSKKAQDNCCRREPLLVRRNQKLSSVEEDPGANQERKVEVIKPLVVEAETTSRATFVLGASVASPPSALGTQEPGIELPIEPRPNEECLQILENAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSTKLAEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDGKEYQVPMATTEGCLVASTNRGCRAISLGGGASSRVLADGMTRGPVVRLPRACDSAEVKTWLETPEGFAVIKEAFDSTSRFARLQKLHVTMAGRNLYIRFQSRTGDAMGMNMISKGTEKALLKLQEFFPDMQILAVSGNYCTDKKPAAINWIEGRGKTVVCEAVIPAKVVREVLKTTTEAMVDVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVRSHMVHNRSKINLQDLQGTCTKKAA

Target ORF information:

RefSeq Version	XM_006517530.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517530.1

ORF Insert Sequence:

ATGCGAGGAT CCAAGGACTG TGAGGCTACA ATGTTGTCAA GACTTTTCCG GATGCATGGC 
CTCTTCGTGG CCTCCCACCC CTGGGAAGTT ATTGTGGGAA CAGTGACACT TACAATCTGT 
ATGATGTCCA TGAACATGTT CACCGGCAAC AACAAGATCT GTGGCTGGAA TTATGAGTGC 
CCTAAATTTG AAGAGGACGT GCTGAGCAGC GACATCATCA TCCTGACGAT AACGCGGTGC 
ATCGCCATCC TGTACATTTA CTTCCAGTTC CAGAACCTAC GGCAGCTTGG GTCCAAGTAC 
ATTCTGGGTA TTGCTGGCCT CTTCACAATT TTCTCAAGCT TTGTCTTCAG TACAGTCGTC 
ATTCATTTCC TCGACAAAGA ACTGACAGGC TTAAATGAAG CTTTGCCCTT TTTTCTGCTC 
TTGATTGACC TTTCTAGAGC GAGTGCATTA GCAAAGTTTG CCCTCAGTTC AAATTCACAG 
GATGAAGTAA GGGAAAATAT AGCTCGTGGA ATGGCAATCC TGGGCCCCAC ATTCACTCTT 
GACGCTCTTG TGGAATGCCT TGTGATTGGA GTTGGCACCA TGTCAGGCGT CCGCCAGCTG 
GAGATCATGT GCTGCTTCGG CTGCATGTCA GTGTTGGCCA ACTACTTTGT GTTCATGACA 
TTCTTCCCGG CCTGTGTGTC GCTGGTCCTA GAGCTTTCTC GTGAAAGCCG TGAGGGTCGT 
CCAATTTGGC AGCTCAGCCA TTTTGCCAGA GTTTTAGAAG AAGAAGAAAA TAAACCAAAC 
CCCGTAACCC AAAGGGTCAA GATGATTATG TCTTTAGGCT TGGTCCTTGT TCACGCTCAT 
AGTCGCTGGA TAGCTGATCC TTCTCCTCAG AACAGCACAG CGGAGCAGGC TAAGGTTTCC 
TTGGGTCTGG ACGAAGATGT GTCCAAGAGA ATTGAACCAA GTGTTTCTCT CTGGCAGTTT 
TATCTCTCCA AGATGATCAG CATGGACATT GAGCAAGTGA TTACCCTGAG TTTAGCCTTC 
CTTTTGGCTG TCAAGTATAT TTTCTTTGAA CAAGCAGAGA CAGAATCGAC ACTGTCATTA 
AAAAATCCTA TCACGTCTCC CGTCGTGACC TCAAAGAAAG CTCAAGACAA CTGTTGTAGA 
CGTGAGCCTC TGCTTGTGAG AAGGAACCAG AAGCTTTCGT CAGTAGAGGA GGATCCAGGA 
GCGAACCAAG AGAGAAAAGT TGAGGTTATA AAACCATTAG TGGTGGAAGC AGAGACTACA 
AGCAGAGCTA CGTTTGTGCT TGGGGCTTCT GTAGCCAGCC CTCCATCCGC CCTGGGGACA 
CAGGAGCCTG GAATTGAACT CCCCATCGAG CCACGACCTA ATGAAGAATG TCTGCAGATA 
CTGGAGAATG CAGAGAAAGG TGCAAAGTTC CTTAGTGATG CAGAGATCAT CCAGTTGGTC 
AATGCCAAGC ATATCCCAGC TTACAAATTG GAAACTCTAA TGGAAACTCA TGAACGTGGT 
GTGTCTATTC GCCGGCAGCT CCTCTCCACA AAGCTTGCAG AGCCGTCCTC TCTACAGTAC 
CTGCCTTACA GAGATTATAA TTATTCCTTG GTGATGGGAG CTTGCTGTGA GAATGTGATC 
GGATATATGC CCATCCCTGT TGGAGTGGCA GGGCCTCTGT GCCTGGATGG GAAGGAGTAC 
CAGGTGCCGA TGGCAACGAC GGAAGGCTGT CTTGTGGCCA GCACTAACAG AGGCTGCAGA 
GCCATAAGCC TTGGTGGAGG TGCCAGCAGC CGGGTCCTTG CAGATGGGAT GACTCGAGGC 
CCAGTGGTGC GTCTTCCACG TGCTTGTGAC TCTGCAGAAG TAAAGACCTG GCTTGAAACA 
CCTGAAGGGT TTGCAGTGAT AAAGGAGGCC TTTGATAGCA CCAGCAGATT TGCTCGTCTA 
CAGAAACTCC ACGTGACGAT GGCAGGACGC AACCTCTATA TCCGTTTCCA GTCCAGGACG 
GGGGACGCCA TGGGCATGAA CATGATCTCT AAGGGTACGG AGAAAGCACT GCTGAAGCTT 
CAGGAGTTCT TTCCTGACAT GCAGATTCTG GCAGTCAGTG GGAACTATTG CACCGACAAG 
AAGCCTGCTG CCATAAATTG GATCGAAGGA CGAGGAAAGA CTGTGGTTTG TGAAGCCGTC 
ATTCCAGCCA AGGTGGTGAG AGAGGTGTTA AAGACAACTA CGGAAGCTAT GGTTGACGTA 
AACATTAACA AGAATCTTGT GGGCTCGGCC ATGGCTGGGA GCATAGGCGG CTACAACGCC 
CACGCAGCAA ACATTGTCAC TGCTATCTAC ATCGCATGTG GCCAGGATGC AGCACAGAAT 
GTGGGGAGTT CAAACTGTAT TACTTTAATG GAAGCCAGTG GTCCCACAAA TGAAGACTTA 
TATATCAGCT GCACCATGCC ATCGATAGAG ATAGGAACCG TGGGTGGTGG GACCAACCTT 
CTACCTCAGC AAGCCTGCCT GCAGATGCTA GGTGTTCAAG GAGCATGCAA AGACAATCCT 
GGAGAAAACG CACGGCAGCT TGCCCGAATT GTATGTGGCA CTGTGATGGC TGGTGAGCTG 
TCCTTGATGG CAGCCTTGGC AGCAGGACAT CTTGTCAGAA GTCACATGGT TCACAACAGA 
TCAAAGATAA ATTTACAAGA TCTTCAAGGA ACGTGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Hmgcr cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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