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IL10 cDNA ORF clone, Mus musculus(House mouse)

Gene Symbol IL10
Entrez Gene ID 16153
Full Name interleukin 10
Synonyms CSIF,Il-10
General protein information
Preferred Names

interleukin 10

Names

interleukin-10
cytokine synthesis inhibitory factor

Gene Type protein-coding
Organism Mus musculus(House mouse)
Genome

1

1 E4|1 56.89 cM


mRNA and Protein(s)

mRNA Protein Name
NM_010548.2 NP_034678.1 interleukin-10 precursor



Homo sapiens (human) IL10 NP_000563.1
Canis lupus familiaris (dog) IL10 NP_001003077.1
Mus musculus (house mouse) Il10 NP_034678.1
Rattus norvegicus (Norway rat) Il10 NP_036986.2
Xenopus tropicalis (tropical clawed frog) il10 NP_001165400.1
Bos taurus (cattle) IL10 NP_776513.1
Gallus gallus (chicken) IL10 NP_001004414.2
Macaca mulatta (rhesus monkey) IL10 NP_001038192.1
Pan troglodytes (chimpanzee) IL10 NP_001129092.2
Danio rerio (zebrafish) il10 NP_001018621.2


Related articles in PubMed

pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity.
Zorzella-Pezavento SF, Chiuso-Minicucci F, França TG, Ishikawa LL, da Rosa LC, Colavite PM, Balbino B, Marques C, Ikoma MR, Masson AP, Silva CL, Sartori A
Journal of immunology research20176257958(2017)

Control of inflammation by stromal Hedgehog pathway activation restrains colitis.
Lee JJ, Rothenberg ME, Seeley ES, Zimdahl B, Kawano S, Lu WJ, Shin K, Sakata-Kato T, Chen JK, Diehn M, Clarke MF, Beachy PA
Proceedings of the National Academy of Sciences of the United States of America113(47)E7545-E7553(2016 Nov)

Role of IL-10 and TNF-α during Mycobacterium tuberculosis infection in murine alveolar macrophages.
Shi J, Sun BH, Zhou LR, Wang XS
Genetics and molecular research : GMR15(3)(2016 Sep)

Identification of a macrophage-specific chromatin signature in the IL-10 locus.
Saraiva M, Christensen JR, Tsytsykova AV, Goldfeld AE, Ley SC, Kioussis D, O'Garra A
Journal of immunology (Baltimore, Md. : 1950)175(2)1041-6(2005 Jul)

Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones.
Fiorentino DF, Bond MW, Mosmann TR
The Journal of experimental medicine170(6)2081-95(1989 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we report that IL-10 is essential for anti-Plasmodium humoral immunity
Title: Cutting Edge: IL-10 Is Essential for the Generation of Germinal Center B Cell Responses and Anti-Plasmodium Humoral Immunity.

this studies provide information on how IL-10 may contribute to optimized parasite control and prevention of immune-mediated pathology during repeated malaria infections
Title: Long-Lived CD4+IFN-γ+ T Cells rather than Short-Lived CD4+IFN-γ+IL-10+ T Cells Initiate Rapid IL-10 Production To Suppress Anamnestic T Cell Responses during Secondary Malaria Infection.

Data (including data from studies in knockout mice) suggest that up-regulation of signaling in colonic mucosa via interleukin-10/microRNA-155/SHIP-1 (Src homology 2 domain-containing inositol-5-phosphatase) is involved in development of colitis due to dysbiosis.
Title: IL-10/microRNA-155/SHIP-1 signaling pathway is crucial for commensal bacteria induced spontaneous colitis.

Lactobacillus curvatus WiKim38 induced significantly higher levels of IL-10 in bone marrow-derived dendritic cells.
Title: Lactobacillus curvatus WiKim38 isolated from kimchi induces IL-10 production in dendritic cells and alleviates DSS-induced colitis in mice.

These results suggest that activation of iNKT cells increases the initial severity of C. albicans infection, most likely mediated by IL-10 induced modulation of macrophage antifungal activity.
Title: Activation of murine invariant NKT cells promotes susceptibility to candidiasis by IL-10 induced modulation of phagocyte antifungal activity.

The following IL10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OMu22605 NM_010548.2
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Mus musculus interleukin 10 (Il10), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu22605
Clone ID Related Accession (Same CDS sequence) NM_010548.2
Accession Version NM_010548.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Mus musculus(House mouse)
Product interleukin-10 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK152344.1 and AL513351.26. On Mar 26, 2010 this sequence version replaced NM_010548.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK152344.1, BC120612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
ATGCCTGGCT CAGCACTGCT ATGCTGCCTG CTCTTACTGA CTGGCATGAG GATCAGCAGG 
GGCCAGTACA GCCGGGAAGA CAATAACTGC ACCCACTTCC CAGTCGGCCA GAGCCACATG
CTCCTAGAGC TGCGGACTGC CTTCAGCCAG GTGAAGACTT TCTTTCAAAC AAAGGACCAG
CTGGACAACA TACTGCTAAC CGACTCCTTA ATGCAGGACT TTAAGGGTTA CTTGGGTTGC
CAAGCCTTAT CGGAAATGAT CCAGTTTTAC CTGGTAGAAG TGATGCCCCA GGCAGAGAAG
CATGGCCCAG AAATCAAGGA GCATTTGAAT TCCCTGGGTG AGAAGCTGAA GACCCTCAGG
ATGCGGCTGA GGCGCTGTCA TCGATTTCTC CCCTGTGAAA ATAAGAGCAA GGCAGTGGAG
CAGGTGAAGA GTGATTTTAA TAAGCTCCAA GACCAAGGTG TCTACAAGGC CATGAATGAA
TTTGACATCT TCATCAACTG CATAGAAGCA TACATGATGA TCAAAATGAA AAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_034678.1
CDS68..604
Misc Feature(1)164..589
Exon (1)1..232
Exon (2)233..292
Exon (3)293..445
Exon (4)446..511
Exon (5)512..1306
Translation

Target ORF information:

RefSeq Version NM_010548.2
Organism Mus musculus(House mouse)
Definition Mus musculus interleukin 10 (Il10), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_010548.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCCTGGCT CAGCACTGCT ATGCTGCCTG CTCTTACTGA CTGGCATGAG GATCAGCAGG 
GGCCAGTACA GCCGGGAAGA CAATAACTGC ACCCACTTCC CAGTCGGCCA GAGCCACATG
CTCCTAGAGC TGCGGACTGC CTTCAGCCAG GTGAAGACTT TCTTTCAAAC AAAGGACCAG
CTGGACAACA TACTGCTAAC CGACTCCTTA ATGCAGGACT TTAAGGGTTA CTTGGGTTGC
CAAGCCTTAT CGGAAATGAT CCAGTTTTAC CTGGTAGAAG TGATGCCCCA GGCAGAGAAG
CATGGCCCAG AAATCAAGGA GCATTTGAAT TCCCTGGGTG AGAAGCTGAA GACCCTCAGG
ATGCGGCTGA GGCGCTGTCA TCGATTTCTC CCCTGTGAAA ATAAGAGCAA GGCAGTGGAG
CAGGTGAAGA GTGATTTTAA TAAGCTCCAA GACCAAGGTG TCTACAAGGC CATGAATGAA
TTTGACATCT TCATCAACTG CATAGAAGCA TACATGATGA TCAAAATGAA AAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity.
Journal of immunology research20176257958(2017)
Zorzella-Pezavento SF,Chiuso-Minicucci F,França TG,Ishikawa LL,da Rosa LC,Colavite PM,Balbino B,Marques C,Ikoma MR,Masson AP,Silva CL,Sartori A


book

Control of inflammation by stromal Hedgehog pathway activation restrains colitis.
Proceedings of the National Academy of Sciences of the United States of America113(47)E7545-E7553(2016 Nov)
Lee JJ,Rothenberg ME,Seeley ES,Zimdahl B,Kawano S,Lu WJ,Shin K,Sakata-Kato T,Chen JK,Diehn M,Clarke MF,Beachy PA


book

Role of IL-10 and TNF-α during Mycobacterium tuberculosis infection in murine alveolar macrophages.
Genetics and molecular research : GMR15(3)(2016 Sep)
Shi J,Sun BH,Zhou LR,Wang XS


book

Identification of a macrophage-specific chromatin signature in the IL-10 locus.
Journal of immunology (Baltimore, Md. : 1950)175(2)1041-6(2005 Jul)
Saraiva M,Christensen JR,Tsytsykova AV,Goldfeld AE,Ley SC,Kioussis D,O'Garra A


book

Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones.
The Journal of experimental medicine170(6)2081-95(1989 Dec)
Fiorentino DF,Bond MW,Mosmann TR


 
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