Impact cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Impact
Entrez Gene ID	16210
Full Name	impact, RWD domain protein
Synonyms	E430016J11Rik
General protein information	Preferred Names impact, RWD domain protein Names protein IMPACT imprinted and ancient gene protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_011246850.3	XP_011245152.1	protein IMPACT isoform X1
NM_001357396.1	NP_001344325.1	protein IMPACT isoform 2
XM_011246849.2	XP_011245151.1	protein IMPACT isoform X1
NM_008378.2	NP_032404.1	protein IMPACT
NM_008378.3	NP_032404.1	protein IMPACT isoform 1
XM_011246850.2	XP_011245152.1	protein IMPACT isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	IMPACT	XP_512067.3
Canis lupus familiaris (dog)	IMPACT	XP_005623055.1
Bos taurus (cattle)	IMPACT	NP_001095971.1
Rattus norvegicus (Norway rat)	Impact	NP_001012235.1
Neurospora crassa	NCU09542	XP_958761.1
Danio rerio (zebrafish)	impact	NP_001005595.1
Homo sapiens (human)	IMPACT	NP_060909.1
Eremothecium gossypii	AGOS_AAR055W	NP_982596.1
Schizosaccharomyces pombe (fission yeast)	SPAC27E2.02	NP_594402.1
Magnaporthe oryzae (rice blast fungus)	MGG_09525	XP_003712215.1
	IMPACT	XP_001095925.2
Saccharomyces cerevisiae (baker's yeast)	YIH1	NP_009985.1
Kluyveromyces lactis	KLLA0C08932g	XP_452597.1
Mus musculus (house mouse)	Impact	NP_032404.1
Gallus gallus (chicken)	IMPACT	XP_419166.3
Caenorhabditis elegans (roundworm)	CELE_Y52B11A.2	NP_001021782.1
Xenopus tropicalis (tropical clawed frog)	impact	NP_001011308.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2.
Cambiaghi TD, Pereira CM, Shanmugam R, Bolech M, Wek RC, Sattlegger E, Castilho BA
Biochemical and biophysical research communications443(2)592-7(2014 Jan)

Role of Tet1 in erasure of genomic imprinting.
Yamaguchi S, Shen L, Liu Y, Sendler D, Zhang Y
Nature504(7480)460-4(2013 Dec)

IMPACT is a developmentally regulated protein in neurons that opposes the eukaryotic initiation factor 2? kinase GCN2 in the modulation of neurite outgrowth.
Roff? M, Hajj GN, Azevedo HF, Alves VS, Castilho BA
The Journal of biological chemistry288(15)10860-9(2013 Apr)

Evidence that Yih1 resides in a complex with ribosomes.
Waller T, Lee SJ, Sattlegger E
The FEBS journal279(10)1761-76(2012 May)

A conditional knockout resource for the genome-wide study of mouse gene function.
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A
Nature474(7351)337-42(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study extends our understanding of the roles played by GCN1 in GCN2 activation induced by a variety of stress arrangements and suggests that IMPACT and YIH1 use similar mechanisms for regulating this eIF2alpha kinase.
Title: Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2.

the participation of the GCN2-IMPACT module of translational regulation in a highly controlled step in the development of the nervous system.
Title: IMPACT is a developmentally regulated protein in neurons that opposes the eukaryotic initiation factor 2? kinase GCN2 in the modulation of neurite outgrowth.

IMPACT ensures constant high levels of translation and low levels of CREB-2 and GADD53 in specific neuronal cells under amino acid starvation conditions.
Title: IMPACT, a protein preferentially expressed in the mouse brain, binds GCN1 and inhibits GCN2 activation.

segmental duplication followed by enhancement of the promoter activity is responsible for the species-specific imprinting of Impact
Title: An evolutionary scenario for genomic imprinting of Impact lying between nonimprinted neighbors.

The following Impact gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Impact cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18306	NM_008378.3 Latest version!	Mus musculus impact, RWD domain protein (Impact), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu54404	NM_001357396.1 Latest version!	Mus musculus impact, RWD domain protein (Impact), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu54404	XM_011246849.2 Latest version!	Mus musculus impact, RWD domain protein (Impact), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu54404	XM_011246850.3 Latest version!	Mus musculus impact, RWD domain protein (Impact), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu18306	NM_008378.2	Mus musculus impact, RWD domain protein (Impact), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu54404	XM_011246850.2	Mus musculus impact, RWD domain protein (Impact), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Impact ( NM_008378.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032404.1 Mus musculus impact, RWD domain protein (Impact), mRNA.

Price & Availability↑

CloneID	OMu18306
Clone ID Related Accession (Same CDS sequence)	NM_008378.2 , NM_008378.3
Accession Version	NM_008378.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	protein IMPACT
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC131672.3. On Nov 17, 2006 this sequence version replaced NM_008378.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D87973.1, BC020524.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGAAG AGGAAGTAGG GAACAGCCAG AGGCAGAGTG AAGAAATCGA AGCAATGGCA 
GCCATTTATG GCGAGGAGTG GTGTGTCATT GATGAAAATG CCAAAATATT TTGTATTAGA 
GTCACTGACT TCATGGATGA CCCCAAATGG ACACTTTGTT TACAGGTGAT GTTGCCAAGT 
GAGTACCCGG GTACAGCACC GCCTTCTTAT CAGCTGAACG CTCCCTGGCT GAAAGGGCAA 
GAACGCGCAG ACTTATCGAA CAGCCTTGAG GAGATATATG TCCACAACAT GGGTGAATCT 
ATTCTTTACC AGTGGGTGGA GAAAATAAGA GATGCTCTGA TACAGAAATC TCAGATAACC 
GAGCCAGACC CAGATGTCAA GAAGAAAACT GAAGAGGTTG AGGTTGAGTC CGAAGAAGAC 
CCTATTCTAG AGCACCCGCC GGAAAATCCA GTTAAAACAT TGGACCTCAA AATCAGTGAA 
GAAACTCAAC CAGAAACTGA AGAATTGCCT CCAGTTGCTC ATGGCGTTCC TATTACTGAC 
CGAAGGAGCA CTTTTCAGGC TCATGTGGCC CCTGTCGTCT GTCCTGAGCA GGTGAAACTG 
GTTCTTGCCA AGCTGTACGA GAATAAGAAG ATTGCCAGCG CCACCCACAA CATCTATGCC 
TACAGGATAT TCTGTGAGGA TAAACAGACC TTCTTGCAGG ACTGTGAAGA TGATGGAGAA 
ACAGCTGCTG GGGGCCGCCT TCTTCACCTC ATGGAGATTT TGAATGTGAA GAATGTCATG 
GTGGTGGTGT CCCGCTGGTA TGGAGGGATC CTGCTGGGAC CTGACCGTTT CAAACACATC 
AACAACTGTG CCAGGAACAT TCTGGTGGAG AAGAACTTCA CGAACACTCC TGACGAGTCA 
ACTAAGAATT TGGGGAAAAA GAAGGTGAAG AAAGACAAGA AGAAGAATGA TCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032404.1
CDS	86..1042
Translation	MAEEEVGNSQRQSEEIEAMAAIYGEEWCVIDENAKIFCIRVTDFMDDPKWTLCLQVMLPSEYPGTAPPSYQLNAPWLKGQERADLSNSLEEIYVHNMGESILYQWVEKIRDALIQKSQITEPDPDVKKKTEEVEVESEEDPILEHPPENPVKTLDLKISEETQPETEELPPVAHGVPITDRRSTFQAHVAPVVCPEQVKLVLAKLYENKKIASATHNIYAYRIFCEDKQTFLQDCEDDGETAAGGRLLHLMEILNVKNVMVVVSRWYGGILLGPDRFKHINNCARNILVEKNFTNTPDESTKNLGKKKVKKDKKKNDH

Target ORF information:

RefSeq Version	NM_008378.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus impact, RWD domain protein (Impact), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008378.2

ORF Insert Sequence:

ATGGCTGAAG AGGAAGTAGG GAACAGCCAG AGGCAGAGTG AAGAAATCGA AGCAATGGCA 
GCCATTTATG GCGAGGAGTG GTGTGTCATT GATGAAAATG CCAAAATATT TTGTATTAGA 
GTCACTGACT TCATGGATGA CCCCAAATGG ACACTTTGTT TACAGGTGAT GTTGCCAAGT 
GAGTACCCGG GTACAGCACC GCCTTCTTAT CAGCTGAACG CTCCCTGGCT GAAAGGGCAA 
GAACGCGCAG ACTTATCGAA CAGCCTTGAG GAGATATATG TCCACAACAT GGGTGAATCT 
ATTCTTTACC AGTGGGTGGA GAAAATAAGA GATGCTCTGA TACAGAAATC TCAGATAACC 
GAGCCAGACC CAGATGTCAA GAAGAAAACT GAAGAGGTTG AGGTTGAGTC CGAAGAAGAC 
CCTATTCTAG AGCACCCGCC GGAAAATCCA GTTAAAACAT TGGACCTCAA AATCAGTGAA 
GAAACTCAAC CAGAAACTGA AGAATTGCCT CCAGTTGCTC ATGGCGTTCC TATTACTGAC 
CGAAGGAGCA CTTTTCAGGC TCATGTGGCC CCTGTCGTCT GTCCTGAGCA GGTGAAACTG 
GTTCTTGCCA AGCTGTACGA GAATAAGAAG ATTGCCAGCG CCACCCACAA CATCTATGCC 
TACAGGATAT TCTGTGAGGA TAAACAGACC TTCTTGCAGG ACTGTGAAGA TGATGGAGAA 
ACAGCTGCTG GGGGCCGCCT TCTTCACCTC ATGGAGATTT TGAATGTGAA GAATGTCATG 
GTGGTGGTGT CCCGCTGGTA TGGAGGGATC CTGCTGGGAC CTGACCGTTT CAAACACATC 
AACAACTGTG CCAGGAACAT TCTGGTGGAG AAGAACTTCA CGAACACTCC TGACGAGTCA 
ACTAAGAATT TGGGGAAAAA GAAGGTGAAG AAAGACAAGA AGAAGAATGA TCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Impact ( NM_008378.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032404.1 Mus musculus impact, RWD domain protein (Impact), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18306
Clone ID Related Accession (Same CDS sequence)	NM_008378.2 , NM_008378.3
Accession Version	NM_008378.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	protein IMPACT isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ056911.1, AK138136.1, AC131672.3 and BM899561.1. On Oct 26, 2017 this sequence version replaced NM_008378.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC020524.1, SRR1660811.216359.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGAAG AGGAAGTAGG GAACAGCCAG AGGCAGAGTG AAGAAATCGA AGCAATGGCA 
GCCATTTATG GCGAGGAGTG GTGTGTCATT GATGAAAATG CCAAAATATT TTGTATTAGA 
GTCACTGACT TCATGGATGA CCCCAAATGG ACACTTTGTT TACAGGTGAT GTTGCCAAGT 
GAGTACCCGG GTACAGCACC GCCTTCTTAT CAGCTGAACG CTCCCTGGCT GAAAGGGCAA 
GAACGCGCAG ACTTATCGAA CAGCCTTGAG GAGATATATG TCCACAACAT GGGTGAATCT 
ATTCTTTACC AGTGGGTGGA GAAAATAAGA GATGCTCTGA TACAGAAATC TCAGATAACC 
GAGCCAGACC CAGATGTCAA GAAGAAAACT GAAGAGGTTG AGGTTGAGTC CGAAGAAGAC 
CCTATTCTAG AGCACCCGCC GGAAAATCCA GTTAAAACAT TGGACCTCAA AATCAGTGAA 
GAAACTCAAC CAGAAACTGA AGAATTGCCT CCAGTTGCTC ATGGCGTTCC TATTACTGAC 
CGAAGGAGCA CTTTTCAGGC TCATGTGGCC CCTGTCGTCT GTCCTGAGCA GGTGAAACTG 
GTTCTTGCCA AGCTGTACGA GAATAAGAAG ATTGCCAGCG CCACCCACAA CATCTATGCC 
TACAGGATAT TCTGTGAGGA TAAACAGACC TTCTTGCAGG ACTGTGAAGA TGATGGAGAA 
ACAGCTGCTG GGGGCCGCCT TCTTCACCTC ATGGAGATTT TGAATGTGAA GAATGTCATG 
GTGGTGGTGT CCCGCTGGTA TGGAGGGATC CTGCTGGGAC CTGACCGTTT CAAACACATC 
AACAACTGTG CCAGGAACAT TCTGGTGGAG AAGAACTTCA CGAACACTCC TGACGAGTCA 
ACTAAGAATT TGGGGAAAAA GAAGGTGAAG AAAGACAAGA AGAAGAATGA TCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032404.1
CDS	415..1371
Translation	MAEEEVGNSQRQSEEIEAMAAIYGEEWCVIDENAKIFCIRVTDFMDDPKWTLCLQVMLPSEYPGTAPPSYQLNAPWLKGQERADLSNSLEEIYVHNMGESILYQWVEKIRDALIQKSQITEPDPDVKKKTEEVEVESEEDPILEHPPENPVKTLDLKISEETQPETEELPPVAHGVPITDRRSTFQAHVAPVVCPEQVKLVLAKLYENKKIASATHNIYAYRIFCEDKQTFLQDCEDDGETAAGGRLLHLMEILNVKNVMVVVSRWYGGILLGPDRFKHINNCARNILVEKNFTNTPDESTKNLGKKKVKKDKKKNDH

Target ORF information:

RefSeq Version	NM_008378.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus impact, RWD domain protein (Impact), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008378.3

ORF Insert Sequence:

ATGGCTGAAG AGGAAGTAGG GAACAGCCAG AGGCAGAGTG AAGAAATCGA AGCAATGGCA 
GCCATTTATG GCGAGGAGTG GTGTGTCATT GATGAAAATG CCAAAATATT TTGTATTAGA 
GTCACTGACT TCATGGATGA CCCCAAATGG ACACTTTGTT TACAGGTGAT GTTGCCAAGT 
GAGTACCCGG GTACAGCACC GCCTTCTTAT CAGCTGAACG CTCCCTGGCT GAAAGGGCAA 
GAACGCGCAG ACTTATCGAA CAGCCTTGAG GAGATATATG TCCACAACAT GGGTGAATCT 
ATTCTTTACC AGTGGGTGGA GAAAATAAGA GATGCTCTGA TACAGAAATC TCAGATAACC 
GAGCCAGACC CAGATGTCAA GAAGAAAACT GAAGAGGTTG AGGTTGAGTC CGAAGAAGAC 
CCTATTCTAG AGCACCCGCC GGAAAATCCA GTTAAAACAT TGGACCTCAA AATCAGTGAA 
GAAACTCAAC CAGAAACTGA AGAATTGCCT CCAGTTGCTC ATGGCGTTCC TATTACTGAC 
CGAAGGAGCA CTTTTCAGGC TCATGTGGCC CCTGTCGTCT GTCCTGAGCA GGTGAAACTG 
GTTCTTGCCA AGCTGTACGA GAATAAGAAG ATTGCCAGCG CCACCCACAA CATCTATGCC 
TACAGGATAT TCTGTGAGGA TAAACAGACC TTCTTGCAGG ACTGTGAAGA TGATGGAGAA 
ACAGCTGCTG GGGGCCGCCT TCTTCACCTC ATGGAGATTT TGAATGTGAA GAATGTCATG 
GTGGTGGTGT CCCGCTGGTA TGGAGGGATC CTGCTGGGAC CTGACCGTTT CAAACACATC 
AACAACTGTG CCAGGAACAT TCTGGTGGAG AAGAACTTCA CGAACACTCC TGACGAGTCA 
ACTAAGAATT TGGGGAAAAA GAAGGTGAAG AAAGACAAGA AGAAGAATGA TCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Impact ( NM_001357396.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344325.1 Mus musculus impact, RWD domain protein (Impact), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu54404
Clone ID Related Accession (Same CDS sequence)	XM_011246850.3 , NM_001357396.1 , XM_011246849.2 , XM_011246850.2
Accession Version	NM_001357396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Mus musculus(house mouse)
Product	protein IMPACT isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131672.3 and BM899561.1. On Oct 26, 2017 this sequence version replaced XM_011246849.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.22193.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCCA TTTATGGCGA GGAGTGGTGT GTCATTGATG AAAATGCCAA AATATTTTGT 
ATTAGAGTCA CTGACTTCAT GGATGACCCC AAATGGACAC TTTGTTTACA GGTGATGTTG 
CCAAGTGAGT ACCCGGGTAC AGCACCGCCT TCTTATCAGC TGAACGCTCC CTGGCTGAAA 
GGGCAAGAAC GCGCAGACTT ATCGAACAGC CTTGAGGAGA TATATGTCCA CAACATGGGT 
GAATCTATTC TTTACCAGTG GGTGGAGAAA ATAAGAGATG CTCTGATACA GAAATCTCAG 
ATAACCGAGC CAGACCCAGA TGTCAAGAAG AAAACTGAAG AGGTTGAGGT TGAGTCCGAA 
GAAGACCCTA TTCTAGAGCA CCCGCCGGAA AATCCAGTTA AAACATTGGA CCTCAAAATC 
AGTGAAGAAA CTCAACCAGA AACTGAAGAA TTGCCTCCAG TTGCTCATGG CGTTCCTATT 
ACTGACCGAA GGAGCACTTT TCAGGCTCAT GTGGCCCCTG TCGTCTGTCC TGAGCAGGTG 
AAACTGGTTC TTGCCAAGCT GTACGAGAAT AAGAAGATTG CCAGCGCCAC CCACAACATC 
TATGCCTACA GGATATTCTG TGAGGATAAA CAGACCTTCT TGCAGGACTG TGAAGATGAT 
GGAGAAACAG CTGCTGGGGG CCGCCTTCTT CACCTCATGG AGATTTTGAA TGTGAAGAAT 
GTCATGGTGG TGGTGTCCCG CTGGTATGGA GGGATCCTGC TGGGACCTGA CCGTTTCAAA 
CACATCAACA ACTGTGCCAG GAACATTCTG GTGGAGAAGA ACTTCACGAA CACTCCTGAC 
GAGTCAACTA AGAATTTGGG GAAAAAGAAG GTGAAGAAAG ACAAGAAGAA GAATGATCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344325.1
CDS	95..997
Translation	MAAIYGEEWCVIDENAKIFCIRVTDFMDDPKWTLCLQVMLPSEYPGTAPPSYQLNAPWLKGQERADLSNSLEEIYVHNMGESILYQWVEKIRDALIQKSQITEPDPDVKKKTEEVEVESEEDPILEHPPENPVKTLDLKISEETQPETEELPPVAHGVPITDRRSTFQAHVAPVVCPEQVKLVLAKLYENKKIASATHNIYAYRIFCEDKQTFLQDCEDDGETAAGGRLLHLMEILNVKNVMVVVSRWYGGILLGPDRFKHINNCARNILVEKNFTNTPDESTKNLGKKKVKKDKKKNDH

Target ORF information:

RefSeq Version	NM_001357396.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus impact, RWD domain protein (Impact), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357396.1

ORF Insert Sequence:

ATGGCAGCCA TTTATGGCGA GGAGTGGTGT GTCATTGATG AAAATGCCAA AATATTTTGT 
ATTAGAGTCA CTGACTTCAT GGATGACCCC AAATGGACAC TTTGTTTACA GGTGATGTTG 
CCAAGTGAGT ACCCGGGTAC AGCACCGCCT TCTTATCAGC TGAACGCTCC CTGGCTGAAA 
GGGCAAGAAC GCGCAGACTT ATCGAACAGC CTTGAGGAGA TATATGTCCA CAACATGGGT 
GAATCTATTC TTTACCAGTG GGTGGAGAAA ATAAGAGATG CTCTGATACA GAAATCTCAG 
ATAACCGAGC CAGACCCAGA TGTCAAGAAG AAAACTGAAG AGGTTGAGGT TGAGTCCGAA 
GAAGACCCTA TTCTAGAGCA CCCGCCGGAA AATCCAGTTA AAACATTGGA CCTCAAAATC 
AGTGAAGAAA CTCAACCAGA AACTGAAGAA TTGCCTCCAG TTGCTCATGG CGTTCCTATT 
ACTGACCGAA GGAGCACTTT TCAGGCTCAT GTGGCCCCTG TCGTCTGTCC TGAGCAGGTG 
AAACTGGTTC TTGCCAAGCT GTACGAGAAT AAGAAGATTG CCAGCGCCAC CCACAACATC 
TATGCCTACA GGATATTCTG TGAGGATAAA CAGACCTTCT TGCAGGACTG TGAAGATGAT 
GGAGAAACAG CTGCTGGGGG CCGCCTTCTT CACCTCATGG AGATTTTGAA TGTGAAGAAT 
GTCATGGTGG TGGTGTCCCG CTGGTATGGA GGGATCCTGC TGGGACCTGA CCGTTTCAAA 
CACATCAACA ACTGTGCCAG GAACATTCTG GTGGAGAAGA ACTTCACGAA CACTCCTGAC 
GAGTCAACTA AGAATTTGGG GAAAAAGAAG GTGAAGAAAG ACAAGAAGAA GAATGATCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Impact ( XM_011246849.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245151.1 Mus musculus impact, RWD domain protein (Impact), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54404
Clone ID Related Accession (Same CDS sequence)	XM_011246850.3 , NM_001357396.1 , XM_011246849.2 , XM_011246850.2
Accession Version	XM_011246849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	protein IMPACT isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCA TTTATGGCGA GGAGTGGTGT GTCATTGATG AAAATGCCAA AATATTTTGT 
ATTAGAGTCA CTGACTTCAT GGATGACCCC AAATGGACAC TTTGTTTACA GGTGATGTTG 
CCAAGTGAGT ACCCGGGTAC AGCACCGCCT TCTTATCAGC TGAACGCTCC CTGGCTGAAA 
GGGCAAGAAC GCGCAGACTT ATCGAACAGC CTTGAGGAGA TATATGTCCA CAACATGGGT 
GAATCTATTC TTTACCAGTG GGTGGAGAAA ATAAGAGATG CTCTGATACA GAAATCTCAG 
ATAACCGAGC CAGACCCAGA TGTCAAGAAG AAAACTGAAG AGGTTGAGGT TGAGTCCGAA 
GAAGACCCTA TTCTAGAGCA CCCGCCGGAA AATCCAGTTA AAACATTGGA CCTCAAAATC 
AGTGAAGAAA CTCAACCAGA AACTGAAGAA TTGCCTCCAG TTGCTCATGG CGTTCCTATT 
ACTGACCGAA GGAGCACTTT TCAGGCTCAT GTGGCCCCTG TCGTCTGTCC TGAGCAGGTG 
AAACTGGTTC TTGCCAAGCT GTACGAGAAT AAGAAGATTG CCAGCGCCAC CCACAACATC 
TATGCCTACA GGATATTCTG TGAGGATAAA CAGACCTTCT TGCAGGACTG TGAAGATGAT 
GGAGAAACAG CTGCTGGGGG CCGCCTTCTT CACCTCATGG AGATTTTGAA TGTGAAGAAT 
GTCATGGTGG TGGTGTCCCG CTGGTATGGA GGGATCCTGC TGGGACCTGA CCGTTTCAAA 
CACATCAACA ACTGTGCCAG GAACATTCTG GTGGAGAAGA ACTTCACGAA CACTCCTGAC 
GAGTCAACTA AGAATTTGGG GAAAAAGAAG GTGAAGAAAG ACAAGAAGAA GAATGATCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245151.1
CDS	1879..2781
Translation	MAAIYGEEWCVIDENAKIFCIRVTDFMDDPKWTLCLQVMLPSEYPGTAPPSYQLNAPWLKGQERADLSNSLEEIYVHNMGESILYQWVEKIRDALIQKSQITEPDPDVKKKTEEVEVESEEDPILEHPPENPVKTLDLKISEETQPETEELPPVAHGVPITDRRSTFQAHVAPVVCPEQVKLVLAKLYENKKIASATHNIYAYRIFCEDKQTFLQDCEDDGETAAGGRLLHLMEILNVKNVMVVVSRWYGGILLGPDRFKHINNCARNILVEKNFTNTPDESTKNLGKKKVKKDKKKNDH

Target ORF information:

RefSeq Version	XM_011246849.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus impact, RWD domain protein (Impact), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246849.2

ORF Insert Sequence:

ATGGCAGCCA TTTATGGCGA GGAGTGGTGT GTCATTGATG AAAATGCCAA AATATTTTGT 
ATTAGAGTCA CTGACTTCAT GGATGACCCC AAATGGACAC TTTGTTTACA GGTGATGTTG 
CCAAGTGAGT ACCCGGGTAC AGCACCGCCT TCTTATCAGC TGAACGCTCC CTGGCTGAAA 
GGGCAAGAAC GCGCAGACTT ATCGAACAGC CTTGAGGAGA TATATGTCCA CAACATGGGT 
GAATCTATTC TTTACCAGTG GGTGGAGAAA ATAAGAGATG CTCTGATACA GAAATCTCAG 
ATAACCGAGC CAGACCCAGA TGTCAAGAAG AAAACTGAAG AGGTTGAGGT TGAGTCCGAA 
GAAGACCCTA TTCTAGAGCA CCCGCCGGAA AATCCAGTTA AAACATTGGA CCTCAAAATC 
AGTGAAGAAA CTCAACCAGA AACTGAAGAA TTGCCTCCAG TTGCTCATGG CGTTCCTATT 
ACTGACCGAA GGAGCACTTT TCAGGCTCAT GTGGCCCCTG TCGTCTGTCC TGAGCAGGTG 
AAACTGGTTC TTGCCAAGCT GTACGAGAAT AAGAAGATTG CCAGCGCCAC CCACAACATC 
TATGCCTACA GGATATTCTG TGAGGATAAA CAGACCTTCT TGCAGGACTG TGAAGATGAT 
GGAGAAACAG CTGCTGGGGG CCGCCTTCTT CACCTCATGG AGATTTTGAA TGTGAAGAAT 
GTCATGGTGG TGGTGTCCCG CTGGTATGGA GGGATCCTGC TGGGACCTGA CCGTTTCAAA 
CACATCAACA ACTGTGCCAG GAACATTCTG GTGGAGAAGA ACTTCACGAA CACTCCTGAC 
GAGTCAACTA AGAATTTGGG GAAAAAGAAG GTGAAGAAAG ACAAGAAGAA GAATGATCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Impact ( XM_011246850.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245152.1 Mus musculus impact, RWD domain protein (Impact), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu54404
Clone ID Related Accession (Same CDS sequence)	XM_011246850.3 , NM_001357396.1 , XM_011246849.2 , XM_011246850.2
Accession Version	XM_011246850.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein IMPACT isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCA TTTATGGCGA GGAGTGGTGT GTCATTGATG AAAATGCCAA AATATTTTGT 
ATTAGAGTCA CTGACTTCAT GGATGACCCC AAATGGACAC TTTGTTTACA GGTGATGTTG 
CCAAGTGAGT ACCCGGGTAC AGCACCGCCT TCTTATCAGC TGAACGCTCC CTGGCTGAAA 
GGGCAAGAAC GCGCAGACTT ATCGAACAGC CTTGAGGAGA TATATGTCCA CAACATGGGT 
GAATCTATTC TTTACCAGTG GGTGGAGAAA ATAAGAGATG CTCTGATACA GAAATCTCAG 
ATAACCGAGC CAGACCCAGA TGTCAAGAAG AAAACTGAAG AGGTTGAGGT TGAGTCCGAA 
GAAGACCCTA TTCTAGAGCA CCCGCCGGAA AATCCAGTTA AAACATTGGA CCTCAAAATC 
AGTGAAGAAA CTCAACCAGA AACTGAAGAA TTGCCTCCAG TTGCTCATGG CGTTCCTATT 
ACTGACCGAA GGAGCACTTT TCAGGCTCAT GTGGCCCCTG TCGTCTGTCC TGAGCAGGTG 
AAACTGGTTC TTGCCAAGCT GTACGAGAAT AAGAAGATTG CCAGCGCCAC CCACAACATC 
TATGCCTACA GGATATTCTG TGAGGATAAA CAGACCTTCT TGCAGGACTG TGAAGATGAT 
GGAGAAACAG CTGCTGGGGG CCGCCTTCTT CACCTCATGG AGATTTTGAA TGTGAAGAAT 
GTCATGGTGG TGGTGTCCCG CTGGTATGGA GGGATCCTGC TGGGACCTGA CCGTTTCAAA 
CACATCAACA ACTGTGCCAG GAACATTCTG GTGGAGAAGA ACTTCACGAA CACTCCTGAC 
GAGTCAACTA AGAATTTGGG GAAAAAGAAG GTGAAGAAAG ACAAGAAGAA GAATGATCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245152.1
CDS	884..1786
Translation	MAAIYGEEWCVIDENAKIFCIRVTDFMDDPKWTLCLQVMLPSEYPGTAPPSYQLNAPWLKGQERADLSNSLEEIYVHNMGESILYQWVEKIRDALIQKSQITEPDPDVKKKTEEVEVESEEDPILEHPPENPVKTLDLKISEETQPETEELPPVAHGVPITDRRSTFQAHVAPVVCPEQVKLVLAKLYENKKIASATHNIYAYRIFCEDKQTFLQDCEDDGETAAGGRLLHLMEILNVKNVMVVVSRWYGGILLGPDRFKHINNCARNILVEKNFTNTPDESTKNLGKKKVKKDKKKNDH

Target ORF information:

RefSeq Version	XM_011246850.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus impact, RWD domain protein (Impact), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246850.2

ORF Insert Sequence:

ATGGCAGCCA TTTATGGCGA GGAGTGGTGT GTCATTGATG AAAATGCCAA AATATTTTGT 
ATTAGAGTCA CTGACTTCAT GGATGACCCC AAATGGACAC TTTGTTTACA GGTGATGTTG 
CCAAGTGAGT ACCCGGGTAC AGCACCGCCT TCTTATCAGC TGAACGCTCC CTGGCTGAAA 
GGGCAAGAAC GCGCAGACTT ATCGAACAGC CTTGAGGAGA TATATGTCCA CAACATGGGT 
GAATCTATTC TTTACCAGTG GGTGGAGAAA ATAAGAGATG CTCTGATACA GAAATCTCAG 
ATAACCGAGC CAGACCCAGA TGTCAAGAAG AAAACTGAAG AGGTTGAGGT TGAGTCCGAA 
GAAGACCCTA TTCTAGAGCA CCCGCCGGAA AATCCAGTTA AAACATTGGA CCTCAAAATC 
AGTGAAGAAA CTCAACCAGA AACTGAAGAA TTGCCTCCAG TTGCTCATGG CGTTCCTATT 
ACTGACCGAA GGAGCACTTT TCAGGCTCAT GTGGCCCCTG TCGTCTGTCC TGAGCAGGTG 
AAACTGGTTC TTGCCAAGCT GTACGAGAAT AAGAAGATTG CCAGCGCCAC CCACAACATC 
TATGCCTACA GGATATTCTG TGAGGATAAA CAGACCTTCT TGCAGGACTG TGAAGATGAT 
GGAGAAACAG CTGCTGGGGG CCGCCTTCTT CACCTCATGG AGATTTTGAA TGTGAAGAAT 
GTCATGGTGG TGGTGTCCCG CTGGTATGGA GGGATCCTGC TGGGACCTGA CCGTTTCAAA 
CACATCAACA ACTGTGCCAG GAACATTCTG GTGGAGAAGA ACTTCACGAA CACTCCTGAC 
GAGTCAACTA AGAATTTGGG GAAAAAGAAG GTGAAGAAAG ACAAGAAGAA GAATGATCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Impact ( XM_011246850.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245152.1 Mus musculus impact, RWD domain protein (Impact), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54404
Clone ID Related Accession (Same CDS sequence)	XM_011246850.3 , NM_001357396.1 , XM_011246849.2 , XM_011246850.2
Accession Version	XM_011246850.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein IMPACT isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246850.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCA TTTATGGCGA GGAGTGGTGT GTCATTGATG AAAATGCCAA AATATTTTGT 
ATTAGAGTCA CTGACTTCAT GGATGACCCC AAATGGACAC TTTGTTTACA GGTGATGTTG 
CCAAGTGAGT ACCCGGGTAC AGCACCGCCT TCTTATCAGC TGAACGCTCC CTGGCTGAAA 
GGGCAAGAAC GCGCAGACTT ATCGAACAGC CTTGAGGAGA TATATGTCCA CAACATGGGT 
GAATCTATTC TTTACCAGTG GGTGGAGAAA ATAAGAGATG CTCTGATACA GAAATCTCAG 
ATAACCGAGC CAGACCCAGA TGTCAAGAAG AAAACTGAAG AGGTTGAGGT TGAGTCCGAA 
GAAGACCCTA TTCTAGAGCA CCCGCCGGAA AATCCAGTTA AAACATTGGA CCTCAAAATC 
AGTGAAGAAA CTCAACCAGA AACTGAAGAA TTGCCTCCAG TTGCTCATGG CGTTCCTATT 
ACTGACCGAA GGAGCACTTT TCAGGCTCAT GTGGCCCCTG TCGTCTGTCC TGAGCAGGTG 
AAACTGGTTC TTGCCAAGCT GTACGAGAAT AAGAAGATTG CCAGCGCCAC CCACAACATC 
TATGCCTACA GGATATTCTG TGAGGATAAA CAGACCTTCT TGCAGGACTG TGAAGATGAT 
GGAGAAACAG CTGCTGGGGG CCGCCTTCTT CACCTCATGG AGATTTTGAA TGTGAAGAAT 
GTCATGGTGG TGGTGTCCCG CTGGTATGGA GGGATCCTGC TGGGACCTGA CCGTTTCAAA 
CACATCAACA ACTGTGCCAG GAACATTCTG GTGGAGAAGA ACTTCACGAA CACTCCTGAC 
GAGTCAACTA AGAATTTGGG GAAAAAGAAG GTGAAGAAAG ACAAGAAGAA GAATGATCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245152.1
CDS	9136..10038
Translation	MAAIYGEEWCVIDENAKIFCIRVTDFMDDPKWTLCLQVMLPSEYPGTAPPSYQLNAPWLKGQERADLSNSLEEIYVHNMGESILYQWVEKIRDALIQKSQITEPDPDVKKKTEEVEVESEEDPILEHPPENPVKTLDLKISEETQPETEELPPVAHGVPITDRRSTFQAHVAPVVCPEQVKLVLAKLYENKKIASATHNIYAYRIFCEDKQTFLQDCEDDGETAAGGRLLHLMEILNVKNVMVVVSRWYGGILLGPDRFKHINNCARNILVEKNFTNTPDESTKNLGKKKVKKDKKKNDH

Target ORF information:

RefSeq Version	XM_011246850.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus impact, RWD domain protein (Impact), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246850.3

ORF Insert Sequence:

ATGGCAGCCA TTTATGGCGA GGAGTGGTGT GTCATTGATG AAAATGCCAA AATATTTTGT 
ATTAGAGTCA CTGACTTCAT GGATGACCCC AAATGGACAC TTTGTTTACA GGTGATGTTG 
CCAAGTGAGT ACCCGGGTAC AGCACCGCCT TCTTATCAGC TGAACGCTCC CTGGCTGAAA 
GGGCAAGAAC GCGCAGACTT ATCGAACAGC CTTGAGGAGA TATATGTCCA CAACATGGGT 
GAATCTATTC TTTACCAGTG GGTGGAGAAA ATAAGAGATG CTCTGATACA GAAATCTCAG 
ATAACCGAGC CAGACCCAGA TGTCAAGAAG AAAACTGAAG AGGTTGAGGT TGAGTCCGAA 
GAAGACCCTA TTCTAGAGCA CCCGCCGGAA AATCCAGTTA AAACATTGGA CCTCAAAATC 
AGTGAAGAAA CTCAACCAGA AACTGAAGAA TTGCCTCCAG TTGCTCATGG CGTTCCTATT 
ACTGACCGAA GGAGCACTTT TCAGGCTCAT GTGGCCCCTG TCGTCTGTCC TGAGCAGGTG 
AAACTGGTTC TTGCCAAGCT GTACGAGAAT AAGAAGATTG CCAGCGCCAC CCACAACATC 
TATGCCTACA GGATATTCTG TGAGGATAAA CAGACCTTCT TGCAGGACTG TGAAGATGAT 
GGAGAAACAG CTGCTGGGGG CCGCCTTCTT CACCTCATGG AGATTTTGAA TGTGAAGAAT 
GTCATGGTGG TGGTGTCCCG CTGGTATGGA GGGATCCTGC TGGGACCTGA CCGTTTCAAA 
CACATCAACA ACTGTGCCAG GAACATTCTG GTGGAGAAGA ACTTCACGAA CACTCCTGAC 
GAGTCAACTA AGAATTTGGG GAAAAAGAAG GTGAAGAAAG ACAAGAAGAA GAATGATCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2.
Biochemical and biophysical research communications443(2)592-7(2014 Jan)
Cambiaghi TD,Pereira CM,Shanmugam R,Bolech M,Wek RC,Sattlegger E,Castilho BA

Role of Tet1 in erasure of genomic imprinting.
Nature504(7480)460-4(2013 Dec)
Yamaguchi S,Shen L,Liu Y,Sendler D,Zhang Y

IMPACT is a developmentally regulated protein in neurons that opposes the eukaryotic initiation factor 2? kinase GCN2 in the modulation of neurite outgrowth.
The Journal of biological chemistry288(15)10860-9(2013 Apr)
Roff? M,Hajj GN,Azevedo HF,Alves VS,Castilho BA

Evidence that Yih1 resides in a complex with ribosomes.
The FEBS journal279(10)1761-76(2012 May)
Waller T,Lee SJ,Sattlegger E

A conditional knockout resource for the genome-wide study of mouse gene function.
Nature474(7351)337-42(2011 Jun)
Skarnes WC,Rosen B,West AP,Koutsourakis M,Bushell W,Iyer V,Mujica AO,Thomas M,Harrow J,Cox T,Jackson D,Severin J,Biggs P,Fu J,Nefedov M,de Jong PJ,Stewart AF,Bradley A

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Impact cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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