Man1a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Man1a2
Entrez Gene ID	17156
Full Name	mannosidase, alpha, class 1A, member 2
Synonyms	AI428775,AI528764,AI854422,Man1b,PCR2
General protein information	Preferred Names mannosidase, alpha, class 1A, member 2 Names mannosyl-oligosaccharide 1,2-alpha-mannosidase IB alpha-1,2-mannosidase IB mannosidase 1, beta processing alpha-1,2-mannosidase IB
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_010763.2	NP_034893.1	mannosyl-oligosaccharide 1,2-alpha-mannosidase IB

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAN1A2	XP_513685.2
Magnaporthe oryzae (rice blast fungus)	MGG_11475	XP_003718084.1
Gallus gallus (chicken)	MAN1A2	XP_416490.2
Drosophila melanogaster (fruit fly)	alpha-Man-I	NP_996396.1
	MAN1A2	XP_001113249.1
Canis lupus familiaris (dog)	MAN1A2	XP_005630723.1
Bos taurus (cattle)	MAN1A2	NP_001193806.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000558	XP_001688291.2
Neurospora crassa	NCU02235	XP_959494.1
Homo sapiens (human)	MAN1A2	NP_006690.1
Mus musculus (house mouse)	Man1a2	NP_034893.1
Rattus norvegicus (Norway rat)	Man1a2	NP_001099922.1
Danio rerio (zebrafish)	wu:fj20e02	NP_001243551.1
Caenorhabditis elegans (roundworm)	C52E4.5	NP_001256388.1
Xenopus tropicalis (tropical clawed frog)	man1a2	XP_002943491.1

Gene Ontology
Bibliography

Related articles in PubMed

Insertion of a knockout-first cassette in Ampd1 gene leads to neonatal death by disruption of neighboring genes expression.
Pan Y, Zhang L, Liu Q, Li Y, Guo H, Peng Y, Peng H, Tang B, Hu Z, Zhao J, Xia K, Li JD
Scientific reports635970(2016 Oct)

The architecture of parent-of-origin effects in mice.
Mott R, Yuan W, Kaisaki P, Gan X, Cleak J, Edwards A, Baud A, Flint J
Cell156(1-2)332-42(2014 Jan)

The dynamics of engineered resident proteins in the mammalian Golgi complex relies on cisternal maturation.
Rizzo R, Parashuraman S, Mirabelli P, Puri C, Lucocq J, Luini A
The Journal of cell biology201(7)1027-36(2013 Jun)

Three-dimensional visualization of testis cord morphogenesis, a novel tubulogenic mechanism in development.
Combes AN, Lesieur E, Harley VR, Sinclair AH, Little MH, Wilhelm D, Koopman P
Developmental dynamics : an official publication of the American Association of Anatomists238(5)1033-41(2009 May)

Respiratory distress and neonatal lethality in mice lacking Golgi alpha1,2-mannosidase IB involved in N-glycan maturation.
Tremblay LO, Nagy Kov?cs E, Daniels E, Wong NK, Sutton-Smith M, Morris HR, Dell A, Marcinkiewicz E, Seidah NG, McKerlie C, Herscovics A
The Journal of biological chemistry282(4)2558-66(2007 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Man1a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Man1a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21829	NM_010763.2 Latest version!	Mus musculus mannosidase, alpha, class 1A, member 2 (Man1a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$377.30 $539.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Man1a2 ( NM_010763.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034893.1 Mus musculus mannosidase, alpha, class 1A, member 2 (Man1a2), mRNA.

Price & Availability↑

CloneID	OMu21829
Clone ID Related Accession (Same CDS sequence)	NM_010763.2
Accession Version	NM_010763.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	mannosyl-oligosaccharide 1,2-alpha-mannosidase IB
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK153336.1, AK013515.1, BC068192.1, AK078291.1, AK041117.1, AK078047.1 and CB723579.1. On Oct 29, 2008 this sequence version replaced NM_010763.1. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.177562.1, SRR1660817.102449.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTACCC CAGCGCTGCT GCCCCTCTCT GGTCGTAGGA TACCACCTTT GAACCTGGGG 
CCGCCCTCCT TCCCACATCA CAGGGCTACC TTGAGACTCT CCGAGAAGTT TATCCTTCTC 
CTCATCCTTA GTGCCTTCAT CACCCTGTGT TTTGGGGCAT TCTTCTTCCT TCCAGACTCT 
TCAAAACACA AACGCTTTGA TCTGGGCTTA GAAGATGTGT TAATTCCTCA CGTAGATGCC 
GGCAAAGGAG CTAAAAACCC CGGCGTCTTC CTGATCCATG GACCCGACGA ACACAGACAC 
AGGGAAGAAG AAGAGCGTCT GAGAAATAAG ATTAGAGCTG ACCATGAGAA AGCCCTGGAA 
GAAGCAAAAG AAAAATTAAG AAAGTCAAGA GAGGAAATCC GTGCAGAAAT TCAGACAGAG 
AAAAACAAAG TAGCCCAAGC AATGAAGACA AAAGAGACCA GGGTACTGCC GCCTGTCCCT 
GTCCCACAAC GTGTAGGGGT CAGTGGTGGG GATCCAGAAG ACATGGAGAT CAAGAAGAAA 
AGAGACAAAA TTAAAGAGAT GATGAAACAT GCCTGGGATA ATTACAGAAC ATACGGATGG 
GGACATAATG AACTAAGGCC TATTGCAAGG AAAGGCCATT CCACTAACAT ATTCGGAAGC 
TCACAGATGG GTGCCACCAT AGTGGATGCT TTGGATACCC TTTATATCAT GGGGCTTCAT 
GATGAATTCA TGGATGGGCA AAGATGGATT GAAGAAAACC TTGATTTCAG TGTGAATTCA 
GAAGTGTCTG TCTTTGAAGT TAACATTCGC TTTATTGGAG GGCTCCTCGC TGCATATTAC 
CTGTCAGGAG AGGAAATATT CAAGACTAAA GCAGTGCAGT TGGCTGAGAA ACTCCTTCCT 
GCCTTTAACA CACCTACTGG GATTCCCTGG GCAATGGTGA ACCTGAAAAG TGGAGTAGGT 
CGAAACTGGG GCTGGGCGTC TGCAGGCAGC AGCATCCTGG CTGAGTTCGG CACCCTGCAC 
ATGGAGTTTG TGCACCTCAG CTACTTGACC GGTGACTTGA CTTACTATAA TAAGGTCATG 
CACATTCGGA AACTACTGCA GAAAATGGAA CGCCCAAATG GTCTTTATCC AAATTATTTA 
AACCCAAGAA CAGGGCGCTG GGGTCAGTAT CACACATCAG TTGGTGGTCT GGGAGATAGT 
TTTTATGAAT ACTTACTGAA AGCATGGCTG ATGTCAGATA AAACAGACCA CGAGGCAAGA 
AGGATGTATG ACGATGCTGT TGAGGCTATA GAAAAACATC TTATTAAGAA GTCCCGAGGA 
GGTCTGGTTT TTATTGGAGA ATGGAAGAAT GGACACTTGG AAAGGAAGAT GGGGCACTTG 
GCCTGCTTTG CTGGGGGAAT GTTTGCCCTT GGAGCAGATG GTTCCAGAAA GGATAAAGCT 
GGCCACTACT TAGAACTAGG GGCAGAAATT GCACGAACAT GTCATGAGTC ATATGACAGA 
ACTGCATTGA AACTAGGTCC GGAGTCATTC AAGTTTGATG GTGCAGTGGA AGCCGTGGCT 
GTGCGGCAGG CTGAAAAGTA TTACATCCTT CGTCCAGAAG TAATTGAAAC CTATTGGTAT 
CTATGGCGAT TTACCCACGA CCCAAGATAC AGGCAGTGGG GCTGGGAAGC AGCACTGGCT 
ATTGAGAAGT CGTGCCGGGT CAGCGGTGGG TTTTCTGGTG TCAAGGATGT ATACGCCCCG 
ACCCCTGTGC ATGACGACGT GCAGCAGAGC TTTTTTCTTG CTGAAACATT AAAATACTTG 
TACCTGCTGT TCTCTGGCGA TGACCTTCTA CCTTTAGACC ACTGGGTGTT TAACACAGAG 
GCGCACCCTC TGCCGGTGTT GCGCTTAGCC AACAGCACTC TTTCAGGTAA TCCTGCTGTC 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034893.1
CDS	612..2537
Translation	MTTPALLPLSGRRIPPLNLGPPSFPHHRATLRLSEKFILLLILSAFITLCFGAFFFLPDSSKHKRFDLGLEDVLIPHVDAGKGAKNPGVFLIHGPDEHRHREEEERLRNKIRADHEKALEEAKEKLRKSREEIRAEIQTEKNKVAQAMKTKETRVLPPVPVPQRVGVSGGDPEDMEIKKKRDKIKEMMKHAWDNYRTYGWGHNELRPIARKGHSTNIFGSSQMGATIVDALDTLYIMGLHDEFMDGQRWIEENLDFSVNSEVSVFEVNIRFIGGLLAAYYLSGEEIFKTKAVQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILAEFGTLHMEFVHLSYLTGDLTYYNKVMHIRKLLQKMERPNGLYPNYLNPRTGRWGQYHTSVGGLGDSFYEYLLKAWLMSDKTDHEARRMYDDAVEAIEKHLIKKSRGGLVFIGEWKNGHLERKMGHLACFAGGMFALGADGSRKDKAGHYLELGAEIARTCHESYDRTALKLGPESFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEKSCRVSGGFSGVKDVYAPTPVHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPLPVLRLANSTLSGNPAVR

Target ORF information:

RefSeq Version	NM_010763.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase, alpha, class 1A, member 2 (Man1a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010763.2

ORF Insert Sequence:

ATGACTACCC CAGCGCTGCT GCCCCTCTCT GGTCGTAGGA TACCACCTTT GAACCTGGGG 
CCGCCCTCCT TCCCACATCA CAGGGCTACC TTGAGACTCT CCGAGAAGTT TATCCTTCTC 
CTCATCCTTA GTGCCTTCAT CACCCTGTGT TTTGGGGCAT TCTTCTTCCT TCCAGACTCT 
TCAAAACACA AACGCTTTGA TCTGGGCTTA GAAGATGTGT TAATTCCTCA CGTAGATGCC 
GGCAAAGGAG CTAAAAACCC CGGCGTCTTC CTGATCCATG GACCCGACGA ACACAGACAC 
AGGGAAGAAG AAGAGCGTCT GAGAAATAAG ATTAGAGCTG ACCATGAGAA AGCCCTGGAA 
GAAGCAAAAG AAAAATTAAG AAAGTCAAGA GAGGAAATCC GTGCAGAAAT TCAGACAGAG 
AAAAACAAAG TAGCCCAAGC AATGAAGACA AAAGAGACCA GGGTACTGCC GCCTGTCCCT 
GTCCCACAAC GTGTAGGGGT CAGTGGTGGG GATCCAGAAG ACATGGAGAT CAAGAAGAAA 
AGAGACAAAA TTAAAGAGAT GATGAAACAT GCCTGGGATA ATTACAGAAC ATACGGATGG 
GGACATAATG AACTAAGGCC TATTGCAAGG AAAGGCCATT CCACTAACAT ATTCGGAAGC 
TCACAGATGG GTGCCACCAT AGTGGATGCT TTGGATACCC TTTATATCAT GGGGCTTCAT 
GATGAATTCA TGGATGGGCA AAGATGGATT GAAGAAAACC TTGATTTCAG TGTGAATTCA 
GAAGTGTCTG TCTTTGAAGT TAACATTCGC TTTATTGGAG GGCTCCTCGC TGCATATTAC 
CTGTCAGGAG AGGAAATATT CAAGACTAAA GCAGTGCAGT TGGCTGAGAA ACTCCTTCCT 
GCCTTTAACA CACCTACTGG GATTCCCTGG GCAATGGTGA ACCTGAAAAG TGGAGTAGGT 
CGAAACTGGG GCTGGGCGTC TGCAGGCAGC AGCATCCTGG CTGAGTTCGG CACCCTGCAC 
ATGGAGTTTG TGCACCTCAG CTACTTGACC GGTGACTTGA CTTACTATAA TAAGGTCATG 
CACATTCGGA AACTACTGCA GAAAATGGAA CGCCCAAATG GTCTTTATCC AAATTATTTA 
AACCCAAGAA CAGGGCGCTG GGGTCAGTAT CACACATCAG TTGGTGGTCT GGGAGATAGT 
TTTTATGAAT ACTTACTGAA AGCATGGCTG ATGTCAGATA AAACAGACCA CGAGGCAAGA 
AGGATGTATG ACGATGCTGT TGAGGCTATA GAAAAACATC TTATTAAGAA GTCCCGAGGA 
GGTCTGGTTT TTATTGGAGA ATGGAAGAAT GGACACTTGG AAAGGAAGAT GGGGCACTTG 
GCCTGCTTTG CTGGGGGAAT GTTTGCCCTT GGAGCAGATG GTTCCAGAAA GGATAAAGCT 
GGCCACTACT TAGAACTAGG GGCAGAAATT GCACGAACAT GTCATGAGTC ATATGACAGA 
ACTGCATTGA AACTAGGTCC GGAGTCATTC AAGTTTGATG GTGCAGTGGA AGCCGTGGCT 
GTGCGGCAGG CTGAAAAGTA TTACATCCTT CGTCCAGAAG TAATTGAAAC CTATTGGTAT 
CTATGGCGAT TTACCCACGA CCCAAGATAC AGGCAGTGGG GCTGGGAAGC AGCACTGGCT 
ATTGAGAAGT CGTGCCGGGT CAGCGGTGGG TTTTCTGGTG TCAAGGATGT ATACGCCCCG 
ACCCCTGTGC ATGACGACGT GCAGCAGAGC TTTTTTCTTG CTGAAACATT AAAATACTTG 
TACCTGCTGT TCTCTGGCGA TGACCTTCTA CCTTTAGACC ACTGGGTGTT TAACACAGAG 
GCGCACCCTC TGCCGGTGTT GCGCTTAGCC AACAGCACTC TTTCAGGTAA TCCTGCTGTC 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insertion of a knockout-first cassette in Ampd1 gene leads to neonatal death by disruption of neighboring genes expression.
Scientific reports635970(2016 Oct)
Pan Y,Zhang L,Liu Q,Li Y,Guo H,Peng Y,Peng H,Tang B,Hu Z,Zhao J,Xia K,Li JD

The architecture of parent-of-origin effects in mice.
Cell156(1-2)332-42(2014 Jan)
Mott R,Yuan W,Kaisaki P,Gan X,Cleak J,Edwards A,Baud A,Flint J

The dynamics of engineered resident proteins in the mammalian Golgi complex relies on cisternal maturation.
The Journal of cell biology201(7)1027-36(2013 Jun)
Rizzo R,Parashuraman S,Mirabelli P,Puri C,Lucocq J,Luini A

Three-dimensional visualization of testis cord morphogenesis, a novel tubulogenic mechanism in development.
Developmental dynamics : an official publication of the American Association of Anatomists238(5)1033-41(2009 May)
Combes AN,Lesieur E,Harley VR,Sinclair AH,Little MH,Wilhelm D,Koopman P

Respiratory distress and neonatal lethality in mice lacking Golgi alpha1,2-mannosidase IB involved in N-glycan maturation.
The Journal of biological chemistry282(4)2558-66(2007 Jan)
Tremblay LO,Nagy Kov?cs E,Daniels E,Wong NK,Sutton-Smith M,Morris HR,Dell A,Marcinkiewicz E,Seidah NG,McKerlie C,Herscovics A

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Man1a2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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