Myl1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myl1
Entrez Gene ID	17901
Full Name	myosin, light polypeptide 1
Synonyms	AI325107,MLC1f,MLC3f,Mylf
General protein information	Preferred Names myosin, light polypeptide 1 Names myosin light chain 1/3, skeletal muscle isoform A1 catalytic A2 catalytic MLC1/MLC3 MLC1F/MLC3F myosin light chain A1/A2 myosin light chain alkali 1/2 myosin light chain, alkali, fast skeletal muscle myosin, light polypeptide 1, alkali; atrial, embryonic myosin, light polypeptide 4, alkali; atrial, embryonic
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C3\|1 33.71 cM
Summary	Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two non-phosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006495763.1	XP_006495826.1	myosin light chain 1/3, skeletal muscle isoform isoform X1
XM_011238451.2	XP_011236753.1	myosin light chain 1/3, skeletal muscle isoform isoform X2
NM_001113387.1	NP_001106858.1	myosin light chain 1/3, skeletal muscle isoform isoform 3f
NM_021285.3	NP_067260.1	myosin light chain 1/3, skeletal muscle isoform isoform 1f
XM_011238451.3	XP_011236753.1	myosin light chain 1/3, skeletal muscle isoform isoform X2
XM_006495763.2	XP_006495826.1	myosin light chain 1/3, skeletal muscle isoform isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Myl1	NP_001071124.1
Pan troglodytes (chimpanzee)	MYL1	XP_516064.2
Canis lupus familiaris (dog)	MYL1	XP_005640670.1
Mus musculus (house mouse)	Myl1	NP_067260.1
Homo sapiens (human)	MYL1	NP_524144.1
Bos taurus (cattle)	MYL1	NP_001073046.1
Danio rerio (zebrafish)	myl1	NP_956294.1
Kluyveromyces lactis	KLLA0F10439g	XP_455555.1
Schizosaccharomyces pombe (fission yeast)	cdc4	NP_594947.1
Neurospora crassa	NCU06617	XP_962359.2
Danio rerio (zebrafish)	mylz3	NP_571694.1
Saccharomyces cerevisiae (baker's yeast)	MLC1	NP_011409.1
Magnaporthe oryzae (rice blast fungus)	MGG_09470	XP_003712281.1
Gallus gallus (chicken)	MYL1	NP_001038097.1
Eremothecium gossypii	AGOS_AFL030C	NP_985518.1
Xenopus tropicalis (tropical clawed frog)	myl1	NP_988954.1

Gene Ontology
Bibliography

Related articles in PubMed

cDNA recombinant plasmid complementary to mRNAs for light chains 1 and 3 of mouse skeletal muscle myosin.
Robert B, Weydert A, Caravatti M, Minty A, Cohen A, Daubas P, Gros F, Buckingham M
Proceedings of the National Academy of Sciences of the United States of America79(8)2437-41(1982 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study provides an additional mechanistic link between Angiotensin II and vasoconstriction via SFK-enhanced MLC phosphorylation in smooth muscle cells.
Title: Src Family Kinases (SFK) Mediate Angiotensin II-Induced Myosin Light Chain Phosphorylation and Hypertension.

Data show that Ca(V) 1.2 and 1.3 channels are expressed in lens, regulating phosphorylation of aquaporin-0 and myosin light chain and expression of connexins 50 and 46.
Title: L-type calcium channels play a critical role in maintaining lens transparency by regulating phosphorylation of aquaporin-0 and myosin light chain and expression of connexins.

Results show that the binding force between selenium and cardiac myosin (CM), which consists of two heavy chains (MHC) and two pairs of light chains: MLC1 and MLC2, was 100 times stronger than that of daunorubicin (DNR) and CM.
Title: Effect of selenium on the interaction between daunorubicin and cardiac myosin.

The present work further established the role of MuRF1 in muscle atrophy and for the first time shows that MuRF1 plays a role in muscle fatigue and twitch potentiation.
Title: Modulation of muscle atrophy, fatigue and MLC phosphorylation by MuRF1 as indicated by hindlimb suspension studies on MuRF1-KO mice.

MLC1f expression is reversely related to the proliferation of muscle cells.
Title: Regulation of cell proliferation by fast Myosin light chain 1 in myoblasts derived from extraocular muscle, diaphragm and gastrocnemius.

The following Myl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15379	NM_021285.3 Latest version!	Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant 1f, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	Quote Price
OMu15471	NM_001113387.1 Latest version!	Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant 3f, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	Quote Price
OMu15379	XM_006495763.2 Latest version!	Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	Quote Price
OMu68256	XM_011238451.3 Latest version!	Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	Quote Price
View Old Accession Versions >>
OMu68256	XM_011238451.2	Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	Quote Price
OMu15379	XM_006495763.1	Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myl1 ( NM_021285.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067260.1 Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant 1f, mRNA.

Price & Availability↑

CloneID	OMu15379
Clone ID Related Accession (Same CDS sequence)	XM_006495763.1 , NM_021285.3 , XM_006495763.2
Accession Version	NM_021285.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	myosin light chain 1/3, skeletal muscle isoform isoform 1f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from W87050.1, AK003182.1 and W48198.1. On Jan 13, 2008 this sequence version replaced NM_021285.2. Transcript Variant: This variant (1f) encodes a protein that is 38 aa longer than isoform 3f. Transcript variants 1f and 3f differ in their first two exons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK003182.1, CX733518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACCAA AGAAAGACGT GAAGAAGCCC GCTGCTGCGC CTGCCCCAGC CCCGGCCCCG 
GCCCCTGCCC CAGCCAAACC TAAGGAAGAA AAAATCGATC TGTCTGCTAT TAAGATCGAG 
TTCTCTAAGG AGCAACAGGA GGACTTCAAG GAGGCATTTC TCCTGTTTGA CAGAACAGGT 
GAATGCAAGA TCACCTTAAG TCAGGTGGGA GACGTCCTCC GGGCTCTGGG CACCAATCCC 
ACCAATGCAG AGGTCAAGAA GGTTCTTGGG AACCCCAGCA ATGAAGAGAT GAATGCTAAG 
AAAATCGAGT TTGAACAGTT TCTGCCCATG ATGCAAGCTA TCTCCAACAA CAAGGACCAG 
GGAGGTTATG AAGATTTCGT TGAGGGTCTG CGTGTCTTCG ACAAGGAGGG CAATGGCACC 
GTCATGGGTG CTGAACTCCG CCATGTCCTC GCCACTCTGG GAGAGAAGAT GAAGGAGGAA 
GAGGTAGAAG CGTTGCTGGC AGGCCAGGAG GACTCCAATG GCTGCATCAA CTATGAAGCT 
TTTGTCAAAC ACATCATGTC TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067260.1
CDS	152..718
Translation	MAPKKDVKKPAAAPAPAPAPAPAPAKPKEEKIDLSAIKIEFSKEQQEDFKEAFLLFDRTGECKITLSQVGDVLRALGTNPTNAEVKKVLGNPSNEEMNAKKIEFEQFLPMMQAISNNKDQGGYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMKEEEVEALLAGQEDSNGCINYEAFVKHIMSV

Target ORF information:

RefSeq Version	NM_021285.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant 1f, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021285.3

ORF Insert Sequence:

ATGGCACCAA AGAAAGACGT GAAGAAGCCC GCTGCTGCGC CTGCCCCAGC CCCGGCCCCG 
GCCCCTGCCC CAGCCAAACC TAAGGAAGAA AAAATCGATC TGTCTGCTAT TAAGATCGAG 
TTCTCTAAGG AGCAACAGGA GGACTTCAAG GAGGCATTTC TCCTGTTTGA CAGAACAGGT 
GAATGCAAGA TCACCTTAAG TCAGGTGGGA GACGTCCTCC GGGCTCTGGG CACCAATCCC 
ACCAATGCAG AGGTCAAGAA GGTTCTTGGG AACCCCAGCA ATGAAGAGAT GAATGCTAAG 
AAAATCGAGT TTGAACAGTT TCTGCCCATG ATGCAAGCTA TCTCCAACAA CAAGGACCAG 
GGAGGTTATG AAGATTTCGT TGAGGGTCTG CGTGTCTTCG ACAAGGAGGG CAATGGCACC 
GTCATGGGTG CTGAACTCCG CCATGTCCTC GCCACTCTGG GAGAGAAGAT GAAGGAGGAA 
GAGGTAGAAG CGTTGCTGGC AGGCCAGGAG GACTCCAATG GCTGCATCAA CTATGAAGCT 
TTTGTCAAAC ACATCATGTC TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myl1 ( NM_001113387.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001106858.1 Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant 3f, mRNA.

Price & Availability↑

CloneID	OMu15471
Clone ID Related Accession (Same CDS sequence)	NM_001113387.1
Accession Version	NM_001113387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	myosin light chain 1/3, skeletal muscle isoform isoform 3f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY785713.1, AK009938.1 and W48198.1. Transcript Variant: This variant (3f) encodes a protein that is 38 aa shorter than isoform 1f. Transcript variants 3f and 1f differ in their first two exons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DV062565.1, BY709519.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTTCA GTGCTGACCA GATTGCCGAC TTCAAGGAGG CATTTCTCCT GTTTGACAGA 
ACAGGTGAAT GCAAGATCAC CTTAAGTCAG GTGGGAGACG TCCTCCGGGC TCTGGGCACC 
AATCCCACCA ATGCAGAGGT CAAGAAGGTT CTTGGGAACC CCAGCAATGA AGAGATGAAT 
GCTAAGAAAA TCGAGTTTGA ACAGTTTCTG CCCATGATGC AAGCTATCTC CAACAACAAG 
GACCAGGGAG GTTATGAAGA TTTCGTTGAG GGTCTGCGTG TCTTCGACAA GGAGGGCAAT 
GGCACCGTCA TGGGTGCTGA ACTCCGCCAT GTCCTCGCCA CTCTGGGAGA GAAGATGAAG 
GAGGAAGAGG TAGAAGCGTT GCTGGCAGGC CAGGAGGACT CCAATGGCTG CATCAACTAT 
GAAGCTTTTG TCAAACACAT CATGTCTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106858.1
CDS	89..541
Translation	MSFSADQIADFKEAFLLFDRTGECKITLSQVGDVLRALGTNPTNAEVKKVLGNPSNEEMNAKKIEFEQFLPMMQAISNNKDQGGYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMKEEEVEALLAGQEDSNGCINYEAFVKHIMSV

Target ORF information:

RefSeq Version	NM_001113387.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant 3f, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113387.1

ORF Insert Sequence:

ATGTCCTTCA GTGCTGACCA GATTGCCGAC TTCAAGGAGG CATTTCTCCT GTTTGACAGA 
ACAGGTGAAT GCAAGATCAC CTTAAGTCAG GTGGGAGACG TCCTCCGGGC TCTGGGCACC 
AATCCCACCA ATGCAGAGGT CAAGAAGGTT CTTGGGAACC CCAGCAATGA AGAGATGAAT 
GCTAAGAAAA TCGAGTTTGA ACAGTTTCTG CCCATGATGC AAGCTATCTC CAACAACAAG 
GACCAGGGAG GTTATGAAGA TTTCGTTGAG GGTCTGCGTG TCTTCGACAA GGAGGGCAAT 
GGCACCGTCA TGGGTGCTGA ACTCCGCCAT GTCCTCGCCA CTCTGGGAGA GAAGATGAAG 
GAGGAAGAGG TAGAAGCGTT GCTGGCAGGC CAGGAGGACT CCAATGGCTG CATCAACTAT 
GAAGCTTTTG TCAAACACAT CATGTCTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myl1 ( XM_006495763.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495826.1 Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15379
Clone ID Related Accession (Same CDS sequence)	XM_006495763.1 , NM_021285.3 , XM_006495763.2
Accession Version	XM_006495763.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	myosin light chain 1/3, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCAA AGAAAGACGT GAAGAAGCCC GCTGCTGCGC CTGCCCCAGC CCCGGCCCCG 
GCCCCTGCCC CAGCCAAACC TAAGGAAGAA AAAATCGATC TGTCTGCTAT TAAGATCGAG 
TTCTCTAAGG AGCAACAGGA GGACTTCAAG GAGGCATTTC TCCTGTTTGA CAGAACAGGT 
GAATGCAAGA TCACCTTAAG TCAGGTGGGA GACGTCCTCC GGGCTCTGGG CACCAATCCC 
ACCAATGCAG AGGTCAAGAA GGTTCTTGGG AACCCCAGCA ATGAAGAGAT GAATGCTAAG 
AAAATCGAGT TTGAACAGTT TCTGCCCATG ATGCAAGCTA TCTCCAACAA CAAGGACCAG 
GGAGGTTATG AAGATTTCGT TGAGGGTCTG CGTGTCTTCG ACAAGGAGGG CAATGGCACC 
GTCATGGGTG CTGAACTCCG CCATGTCCTC GCCACTCTGG GAGAGAAGAT GAAGGAGGAA 
GAGGTAGAAG CGTTGCTGGC AGGCCAGGAG GACTCCAATG GCTGCATCAA CTATGAAGCT 
TTTGTCAAAC ACATCATGTC TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495826.1
CDS	289..855
Translation	MAPKKDVKKPAAAPAPAPAPAPAPAKPKEEKIDLSAIKIEFSKEQQEDFKEAFLLFDRTGECKITLSQVGDVLRALGTNPTNAEVKKVLGNPSNEEMNAKKIEFEQFLPMMQAISNNKDQGGYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMKEEEVEALLAGQEDSNGCINYEAFVKHIMSV

Target ORF information:

RefSeq Version	XM_006495763.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495763.1

ORF Insert Sequence:

ATGGCACCAA AGAAAGACGT GAAGAAGCCC GCTGCTGCGC CTGCCCCAGC CCCGGCCCCG 
GCCCCTGCCC CAGCCAAACC TAAGGAAGAA AAAATCGATC TGTCTGCTAT TAAGATCGAG 
TTCTCTAAGG AGCAACAGGA GGACTTCAAG GAGGCATTTC TCCTGTTTGA CAGAACAGGT 
GAATGCAAGA TCACCTTAAG TCAGGTGGGA GACGTCCTCC GGGCTCTGGG CACCAATCCC 
ACCAATGCAG AGGTCAAGAA GGTTCTTGGG AACCCCAGCA ATGAAGAGAT GAATGCTAAG 
AAAATCGAGT TTGAACAGTT TCTGCCCATG ATGCAAGCTA TCTCCAACAA CAAGGACCAG 
GGAGGTTATG AAGATTTCGT TGAGGGTCTG CGTGTCTTCG ACAAGGAGGG CAATGGCACC 
GTCATGGGTG CTGAACTCCG CCATGTCCTC GCCACTCTGG GAGAGAAGAT GAAGGAGGAA 
GAGGTAGAAG CGTTGCTGGC AGGCCAGGAG GACTCCAATG GCTGCATCAA CTATGAAGCT 
TTTGTCAAAC ACATCATGTC TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myl1 ( XM_011238451.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236753.1 Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu68256
Clone ID Related Accession (Same CDS sequence)	XM_011238451.2 , XM_011238451.3
Accession Version	XM_011238451.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	myosin light chain 1/3, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTCA GTGCTGACCA GATTGCCGAC TTCAAGGAGG CATTTCTCCT GTTTGACAGA 
ACAGGTGAAT GCAAGATCAC CTTAAGTCAG GTGGGAGACG TCCTCCGGGC TCTGGGCACC 
AATCCCACCA ATGCAGAGGT CAAGAAGGTT CTTGGGAACC CCAGCAATGA AGAGATGAAT 
GCTAAGAAAA TCGAGTTTGA ACAGTTTCTG CCCATGATGC AAGCTATCTC CAACAACAAG 
GACCAGGGAG GTTATGAAGA TTTCGTTGAG GGTCTGCGTG TCTTCGACAA GGAGGGCAAT 
GGCACCGTCA TGGGTGCTGA ACTCCGCCAT GTCCTCGCCA CTCTGGGAGA GAAGATGAAG 
GAGGAAGAGG TAGAAGCGTT GCTGGCAGGC CAGGAGGACT CCAATGGCTG CATCAACTAT 
GAAGGTACAG CCAGCCTCAC CTCATTAAGG AGAGATTCAT TTTCAACTTT ATTTTCCTCT 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236753.1
CDS	139..624
Translation	MSFSADQIADFKEAFLLFDRTGECKITLSQVGDVLRALGTNPTNAEVKKVLGNPSNEEMNAKKIEFEQFLPMMQAISNNKDQGGYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMKEEEVEALLAGQEDSNGCINYEGTASLTSLRRDSFSTLFSSS

Target ORF information:

RefSeq Version	XM_011238451.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238451.2

ORF Insert Sequence:

ATGTCCTTCA GTGCTGACCA GATTGCCGAC TTCAAGGAGG CATTTCTCCT GTTTGACAGA 
ACAGGTGAAT GCAAGATCAC CTTAAGTCAG GTGGGAGACG TCCTCCGGGC TCTGGGCACC 
AATCCCACCA ATGCAGAGGT CAAGAAGGTT CTTGGGAACC CCAGCAATGA AGAGATGAAT 
GCTAAGAAAA TCGAGTTTGA ACAGTTTCTG CCCATGATGC AAGCTATCTC CAACAACAAG 
GACCAGGGAG GTTATGAAGA TTTCGTTGAG GGTCTGCGTG TCTTCGACAA GGAGGGCAAT 
GGCACCGTCA TGGGTGCTGA ACTCCGCCAT GTCCTCGCCA CTCTGGGAGA GAAGATGAAG 
GAGGAAGAGG TAGAAGCGTT GCTGGCAGGC CAGGAGGACT CCAATGGCTG CATCAACTAT 
GAAGGTACAG CCAGCCTCAC CTCATTAAGG AGAGATTCAT TTTCAACTTT ATTTTCCTCT 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myl1 ( XM_006495763.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495826.1 Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15379
Clone ID Related Accession (Same CDS sequence)	XM_006495763.1 , NM_021285.3 , XM_006495763.2
Accession Version	XM_006495763.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin light chain 1/3, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495763.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCAA AGAAAGACGT GAAGAAGCCC GCTGCTGCGC CTGCCCCAGC CCCGGCCCCG 
GCCCCTGCCC CAGCCAAACC TAAGGAAGAA AAAATCGATC TGTCTGCTAT TAAGATCGAG 
TTCTCTAAGG AGCAACAGGA GGACTTCAAG GAGGCATTTC TCCTGTTTGA CAGAACAGGT 
GAATGCAAGA TCACCTTAAG TCAGGTGGGA GACGTCCTCC GGGCTCTGGG CACCAATCCC 
ACCAATGCAG AGGTCAAGAA GGTTCTTGGG AACCCCAGCA ATGAAGAGAT GAATGCTAAG 
AAAATCGAGT TTGAACAGTT TCTGCCCATG ATGCAAGCTA TCTCCAACAA CAAGGACCAG 
GGAGGTTATG AAGATTTCGT TGAGGGTCTG CGTGTCTTCG ACAAGGAGGG CAATGGCACC 
GTCATGGGTG CTGAACTCCG CCATGTCCTC GCCACTCTGG GAGAGAAGAT GAAGGAGGAA 
GAGGTAGAAG CGTTGCTGGC AGGCCAGGAG GACTCCAATG GCTGCATCAA CTATGAAGCT 
TTTGTCAAAC ACATCATGTC TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495826.1
CDS	1728..2294
Translation	MAPKKDVKKPAAAPAPAPAPAPAPAKPKEEKIDLSAIKIEFSKEQQEDFKEAFLLFDRTGECKITLSQVGDVLRALGTNPTNAEVKKVLGNPSNEEMNAKKIEFEQFLPMMQAISNNKDQGGYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMKEEEVEALLAGQEDSNGCINYEAFVKHIMSV

Target ORF information:

RefSeq Version	XM_006495763.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495763.2

ORF Insert Sequence:

ATGGCACCAA AGAAAGACGT GAAGAAGCCC GCTGCTGCGC CTGCCCCAGC CCCGGCCCCG 
GCCCCTGCCC CAGCCAAACC TAAGGAAGAA AAAATCGATC TGTCTGCTAT TAAGATCGAG 
TTCTCTAAGG AGCAACAGGA GGACTTCAAG GAGGCATTTC TCCTGTTTGA CAGAACAGGT 
GAATGCAAGA TCACCTTAAG TCAGGTGGGA GACGTCCTCC GGGCTCTGGG CACCAATCCC 
ACCAATGCAG AGGTCAAGAA GGTTCTTGGG AACCCCAGCA ATGAAGAGAT GAATGCTAAG 
AAAATCGAGT TTGAACAGTT TCTGCCCATG ATGCAAGCTA TCTCCAACAA CAAGGACCAG 
GGAGGTTATG AAGATTTCGT TGAGGGTCTG CGTGTCTTCG ACAAGGAGGG CAATGGCACC 
GTCATGGGTG CTGAACTCCG CCATGTCCTC GCCACTCTGG GAGAGAAGAT GAAGGAGGAA 
GAGGTAGAAG CGTTGCTGGC AGGCCAGGAG GACTCCAATG GCTGCATCAA CTATGAAGCT 
TTTGTCAAAC ACATCATGTC TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myl1 ( XM_011238451.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236753.1 Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant X2, mRNA.

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CloneID	OMu68256
Clone ID Related Accession (Same CDS sequence)	XM_011238451.2 , XM_011238451.3
Accession Version	XM_011238451.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin light chain 1/3, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238451.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTCA GTGCTGACCA GATTGCCGAC TTCAAGGAGG CATTTCTCCT GTTTGACAGA 
ACAGGTGAAT GCAAGATCAC CTTAAGTCAG GTGGGAGACG TCCTCCGGGC TCTGGGCACC 
AATCCCACCA ATGCAGAGGT CAAGAAGGTT CTTGGGAACC CCAGCAATGA AGAGATGAAT 
GCTAAGAAAA TCGAGTTTGA ACAGTTTCTG CCCATGATGC AAGCTATCTC CAACAACAAG 
GACCAGGGAG GTTATGAAGA TTTCGTTGAG GGTCTGCGTG TCTTCGACAA GGAGGGCAAT 
GGCACCGTCA TGGGTGCTGA ACTCCGCCAT GTCCTCGCCA CTCTGGGAGA GAAGATGAAG 
GAGGAAGAGG TAGAAGCGTT GCTGGCAGGC CAGGAGGACT CCAATGGCTG CATCAACTAT 
GAAGGTACAG CCAGCCTCAC CTCATTAAGG AGAGATTCAT TTTCAACTTT ATTTTCCTCT 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236753.1
CDS	130..615
Translation	MSFSADQIADFKEAFLLFDRTGECKITLSQVGDVLRALGTNPTNAEVKKVLGNPSNEEMNAKKIEFEQFLPMMQAISNNKDQGGYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMKEEEVEALLAGQEDSNGCINYEGTASLTSLRRDSFSTLFSSS

Target ORF information:

RefSeq Version	XM_011238451.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide 1 (Myl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238451.3

ORF Insert Sequence:

ATGTCCTTCA GTGCTGACCA GATTGCCGAC TTCAAGGAGG CATTTCTCCT GTTTGACAGA 
ACAGGTGAAT GCAAGATCAC CTTAAGTCAG GTGGGAGACG TCCTCCGGGC TCTGGGCACC 
AATCCCACCA ATGCAGAGGT CAAGAAGGTT CTTGGGAACC CCAGCAATGA AGAGATGAAT 
GCTAAGAAAA TCGAGTTTGA ACAGTTTCTG CCCATGATGC AAGCTATCTC CAACAACAAG 
GACCAGGGAG GTTATGAAGA TTTCGTTGAG GGTCTGCGTG TCTTCGACAA GGAGGGCAAT 
GGCACCGTCA TGGGTGCTGA ACTCCGCCAT GTCCTCGCCA CTCTGGGAGA GAAGATGAAG 
GAGGAAGAGG TAGAAGCGTT GCTGGCAGGC CAGGAGGACT CCAATGGCTG CATCAACTAT 
GAAGGTACAG CCAGCCTCAC CTCATTAAGG AGAGATTCAT TTTCAACTTT ATTTTCCTCT 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

cDNA recombinant plasmid complementary to mRNAs for light chains 1 and 3 of mouse skeletal muscle myosin.
Proceedings of the National Academy of Sciences of the United States of America79(8)2437-41(1982 Apr)
Robert B,Weydert A,Caravatti M,Minty A,Cohen A,Daubas P,Gros F,Buckingham M

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Myl1 cDNA ORF clone, Mus musculus(house mouse)

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