Nat2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nat2
Entrez Gene ID	17961
Full Name	N-acetyltransferase 2 (arylamine N-acetyltransferase)
Synonyms	AV377607
General protein information	Preferred Names N-acetyltransferase 2 (arylamine N-acetyltransferase) Names arylamine N-acetyltransferase 2 N-acetyl transferase 2 N-acetyltransferase type 2 NAT-2 arylamide acetylase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 B3.2\|8 33.38 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006509592.2	XP_006509655.1	arylamine N-acetyltransferase 2 isoform X1
NM_001168577.1	NP_001162049.1	arylamine N-acetyltransferase 2
NM_010874.3	NP_035004.1	arylamine N-acetyltransferase 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NAT	NP_990857.1
Danio rerio (zebrafish)	zgc:103601	NP_001005974.1
Homo sapiens (human)	NAT1	NP_001153648.1
Pan troglodytes (chimpanzee)	NAT1	XP_519630.2
	NAT1	XP_002805323.1
Gallus gallus (chicken)	PNAT3	NP_001004373.1
Danio rerio (zebrafish)	si:dkey-78a14.5	XP_005169078.1
Rattus norvegicus (Norway rat)	Nat2	NP_446306.1
Bos taurus (cattle)	NAT1	NP_001069040.1
Mus musculus (house mouse)	Nat2	NP_001162049.1
Gallus gallus (chicken)	PNAT10	NP_990671.1
Danio rerio (zebrafish)	zgc:101040	NP_001003588.1
Danio rerio (zebrafish)	LOC794381	XP_005169079.1

Gene Ontology
Bibliography

Related articles in PubMed

Deficiency of N-acetyltransferase increases the interactions of isoniazid with endobiotics in mouse liver.
Wang P, Shehu AI, Lu J, Joshi RH, Venkataramanan R, Sugamori KS, Grant DM, Zhong XB, Ma X
Biochemical pharmacology145218-225(2017 Dec)

Acute murine colitis reduces colonic 5-aminosalicylic acid metabolism by regulation of N-acetyltransferase-2.
Ram?rez-Alc?ntara V, Montrose MH
American journal of physiology. Gastrointestinal and liver physiology306(11)G1002-10(2014 Jun)

From arylamine N-acetyltransferase to folate-dependent acetyl CoA hydrolase: impact of folic acid on the activity of (HUMAN)NAT1 and its homologue (MOUSE)NAT2.
Laurieri N, Dairou J, Egleton JE, Stanley LA, Russell AJ, Dupret JM, Sim E, Rodrigues-Lima F
PloS one9(5)e96370(2014)

5-methyl-tetrahydrofolate and the S-adenosylmethionine cycle in C57BL/6J mouse tissues: gender differences and effects of arylamine N-acetyltransferase-1 deletion.
Witham KL, Butcher NJ, Sugamori KS, Brenneman D, Grant DM, Minchin RF
PloS one8(10)e77923(2013)

Reduced 4-aminobiphenyl-induced liver tumorigenicity but not DNA damage in arylamine N-acetyltransferase null mice.
Sugamori KS, Brenneman D, Sanchez O, Doll MA, Hein DW, Pierce WM Jr, Grant DM
Cancer letters318(2)206-13(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study illustrated that deficiency of NAT1/2 decreases isoniazid (INH) acetylation, but increases the interactions of INH with endobiotics in the liver.
Title: Deficiency of N-acetyltransferase increases the interactions of isoniazid with endobiotics in mouse liver.

It was concluded that an acute colonic inflammation impairs the expression and function of NAT2 enzyme, thereby diminishing the capacity for 5-aminosalisylic acid metabolism by colonic mucosa.
Title: Acute murine colitis reduces colonic 5-aminosalicylic acid metabolism by regulation of N-acetyltransferase-2.

Hyperhomocysteinemia-induced decrease of peroxynitrite level is associated with an increase of hepatic Nat2 isoform activity with a reversal effect of wine polyphenol extract supplementation.
Title: Effect of red wine polyphenol dietary supplementation on two phase II enzymes in liver of hyperhomocysteinemic mice.

Mouse NAT2 is likely to influence epigenetic gene control, particularly of its own locus, and this is consistent with recent evidence associating aberrant mouse Nat2/human NAT1 gene expression with certain developmental malformations and cancers.
Title: Characterisation of CpG methylation in the upstream control region of mouse Nat2: evidence for a gene-environment interaction in a polymorphic gene implicated in folate metabolism.

Mouse N-acetyltransferase-2 was produced as a recombinant protein and found to have a substrate specificity profile similar to human N-acetyltransferase type 1.
Title: Mouse N-acetyltransferase type 2, the homologue of human N-acetyltransferase type 1.

The following Nat2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nat2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07071	NM_001168577.1 Latest version!	Mus musculus N-acetyltransferase 2 (arylamine N-acetyltransferase) (Nat2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu07071	NM_010874.3 Latest version!	Mus musculus N-acetyltransferase 2 (arylamine N-acetyltransferase) (Nat2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu40964	XM_006509592.2 Latest version!	Mus musculus N-acetyltransferase 2 (arylamine N-acetyltransferase) (Nat2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nat2 ( NM_001168577.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001162049.1 Mus musculus N-acetyltransferase 2 (arylamine N-acetyltransferase) (Nat2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07071
Clone ID Related Accession (Same CDS sequence)	NM_001168577.1 , NM_010874.3
Accession Version	NM_001168577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	arylamine N-acetyltransferase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK007583.1 and AC122867.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK007583.1, CN663986.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACATCG AAGCGTACTT TGAAAGAATT GGTTATCAGA GCACCAGGAG CAAACTGGAC 
TTGAAAACAT TAACCGAAAT CCTTCAGCAC CAGATACGGG CTATTCCCTT TGAGAATTTG 
AACATCCATT GTGGGGAATC CATGGAACTG AGTTTAGAAG CCATCTTTGA TCAAATTGTG 
AGGAAGAAGC GGGGTGGATG GTGTCTCCAG GTTAATCATC TGCTGTACTG GGCTCTGACC 
AAACTGGGCT TTGAAACCAC AATGCTGGGA GGATATGTCT TTAACACTCC AGCCAATAAG 
TACAGCAGTG GCATGATTCA CCTTCTAGTT CAGGTCACCA TCAGTGGAAA GGACTACATT 
GTTGATGCTG GGTTTGGACG TTCCTACCAG ATGTGGGAGC CTCTGGAATT AACATCTGGA 
AAGGATCAGC CTCAGGTGCC TGCCATCTTC CGTTTGACAG AGGAGAATGG AACCTGGTAC 
TTGGACCAAA TCAGAAGAGA GCAGTATGTT CCAAACCAAG AATTTATTAA CTCAGATCTC 
CTTGAAAAGA ACAAATACCG AAAAATCTAT TCCTTTACTC TTGAGCCCCG AACTATTGAA 
GATTTTGAGT CCATGAATAC CTACCTTCAG ACATCACCAG CGTCTGTGTT TACTAGCAAA 
TCATTTTGTT CCCTGCAGAC CCCAGAAGGA GTTCATTGTT TGGTTGGCTC CACCCTCACT 
TATAGAAGAT TTAGTTACAA GGACAACGTC GATCTTGTAG AGTTTAAGAG TCTGACTGAG 
GAAGAAATAG AAGATGTACT GAGAACTATA TTTGGGGTTT CTTTAGAGAG AAAACTTGTG 
CCTAAACATG GTGATCGATT TTTTACCATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162049.1
CDS	35..907
Translation	MDIEAYFERIGYQSTRSKLDLKTLTEILQHQIRAIPFENLNIHCGESMELSLEAIFDQIVRKKRGGWCLQVNHLLYWALTKLGFETTMLGGYVFNTPANKYSSGMIHLLVQVTISGKDYIVDAGFGRSYQMWEPLELTSGKDQPQVPAIFRLTEENGTWYLDQIRREQYVPNQEFINSDLLEKNKYRKIYSFTLEPRTIEDFESMNTYLQTSPASVFTSKSFCSLQTPEGVHCLVGSTLTYRRFSYKDNVDLVEFKSLTEEEIEDVLRTIFGVSLERKLVPKHGDRFFTI

Target ORF information:

RefSeq Version	NM_001168577.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acetyltransferase 2 (arylamine N-acetyltransferase) (Nat2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168577.1

ORF Insert Sequence:

ATGGACATCG AAGCGTACTT TGAAAGAATT GGTTATCAGA GCACCAGGAG CAAACTGGAC 
TTGAAAACAT TAACCGAAAT CCTTCAGCAC CAGATACGGG CTATTCCCTT TGAGAATTTG 
AACATCCATT GTGGGGAATC CATGGAACTG AGTTTAGAAG CCATCTTTGA TCAAATTGTG 
AGGAAGAAGC GGGGTGGATG GTGTCTCCAG GTTAATCATC TGCTGTACTG GGCTCTGACC 
AAACTGGGCT TTGAAACCAC AATGCTGGGA GGATATGTCT TTAACACTCC AGCCAATAAG 
TACAGCAGTG GCATGATTCA CCTTCTAGTT CAGGTCACCA TCAGTGGAAA GGACTACATT 
GTTGATGCTG GGTTTGGACG TTCCTACCAG ATGTGGGAGC CTCTGGAATT AACATCTGGA 
AAGGATCAGC CTCAGGTGCC TGCCATCTTC CGTTTGACAG AGGAGAATGG AACCTGGTAC 
TTGGACCAAA TCAGAAGAGA GCAGTATGTT CCAAACCAAG AATTTATTAA CTCAGATCTC 
CTTGAAAAGA ACAAATACCG AAAAATCTAT TCCTTTACTC TTGAGCCCCG AACTATTGAA 
GATTTTGAGT CCATGAATAC CTACCTTCAG ACATCACCAG CGTCTGTGTT TACTAGCAAA 
TCATTTTGTT CCCTGCAGAC CCCAGAAGGA GTTCATTGTT TGGTTGGCTC CACCCTCACT 
TATAGAAGAT TTAGTTACAA GGACAACGTC GATCTTGTAG AGTTTAAGAG TCTGACTGAG 
GAAGAAATAG AAGATGTACT GAGAACTATA TTTGGGGTTT CTTTAGAGAG AAAACTTGTG 
CCTAAACATG GTGATCGATT TTTTACCATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nat2 ( NM_010874.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035004.1 Mus musculus N-acetyltransferase 2 (arylamine N-acetyltransferase) (Nat2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07071
Clone ID Related Accession (Same CDS sequence)	NM_001168577.1 , NM_010874.3
Accession Version	NM_010874.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	arylamine N-acetyltransferase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122867.4 and AK007583.1. On Dec 10, 2009 this sequence version replaced NM_010874.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ251710.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACATCG AAGCGTACTT TGAAAGAATT GGTTATCAGA GCACCAGGAG CAAACTGGAC 
TTGAAAACAT TAACCGAAAT CCTTCAGCAC CAGATACGGG CTATTCCCTT TGAGAATTTG 
AACATCCATT GTGGGGAATC CATGGAACTG AGTTTAGAAG CCATCTTTGA TCAAATTGTG 
AGGAAGAAGC GGGGTGGATG GTGTCTCCAG GTTAATCATC TGCTGTACTG GGCTCTGACC 
AAACTGGGCT TTGAAACCAC AATGCTGGGA GGATATGTCT TTAACACTCC AGCCAATAAG 
TACAGCAGTG GCATGATTCA CCTTCTAGTT CAGGTCACCA TCAGTGGAAA GGACTACATT 
GTTGATGCTG GGTTTGGACG TTCCTACCAG ATGTGGGAGC CTCTGGAATT AACATCTGGA 
AAGGATCAGC CTCAGGTGCC TGCCATCTTC CGTTTGACAG AGGAGAATGG AACCTGGTAC 
TTGGACCAAA TCAGAAGAGA GCAGTATGTT CCAAACCAAG AATTTATTAA CTCAGATCTC 
CTTGAAAAGA ACAAATACCG AAAAATCTAT TCCTTTACTC TTGAGCCCCG AACTATTGAA 
GATTTTGAGT CCATGAATAC CTACCTTCAG ACATCACCAG CGTCTGTGTT TACTAGCAAA 
TCATTTTGTT CCCTGCAGAC CCCAGAAGGA GTTCATTGTT TGGTTGGCTC CACCCTCACT 
TATAGAAGAT TTAGTTACAA GGACAACGTC GATCTTGTAG AGTTTAAGAG TCTGACTGAG 
GAAGAAATAG AAGATGTACT GAGAACTATA TTTGGGGTTT CTTTAGAGAG AAAACTTGTG 
CCTAAACATG GTGATCGATT TTTTACCATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035004.1
CDS	133..1005
Translation	MDIEAYFERIGYQSTRSKLDLKTLTEILQHQIRAIPFENLNIHCGESMELSLEAIFDQIVRKKRGGWCLQVNHLLYWALTKLGFETTMLGGYVFNTPANKYSSGMIHLLVQVTISGKDYIVDAGFGRSYQMWEPLELTSGKDQPQVPAIFRLTEENGTWYLDQIRREQYVPNQEFINSDLLEKNKYRKIYSFTLEPRTIEDFESMNTYLQTSPASVFTSKSFCSLQTPEGVHCLVGSTLTYRRFSYKDNVDLVEFKSLTEEEIEDVLRTIFGVSLERKLVPKHGDRFFTI

Target ORF information:

RefSeq Version	NM_010874.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acetyltransferase 2 (arylamine N-acetyltransferase) (Nat2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010874.3

ORF Insert Sequence:

ATGGACATCG AAGCGTACTT TGAAAGAATT GGTTATCAGA GCACCAGGAG CAAACTGGAC 
TTGAAAACAT TAACCGAAAT CCTTCAGCAC CAGATACGGG CTATTCCCTT TGAGAATTTG 
AACATCCATT GTGGGGAATC CATGGAACTG AGTTTAGAAG CCATCTTTGA TCAAATTGTG 
AGGAAGAAGC GGGGTGGATG GTGTCTCCAG GTTAATCATC TGCTGTACTG GGCTCTGACC 
AAACTGGGCT TTGAAACCAC AATGCTGGGA GGATATGTCT TTAACACTCC AGCCAATAAG 
TACAGCAGTG GCATGATTCA CCTTCTAGTT CAGGTCACCA TCAGTGGAAA GGACTACATT 
GTTGATGCTG GGTTTGGACG TTCCTACCAG ATGTGGGAGC CTCTGGAATT AACATCTGGA 
AAGGATCAGC CTCAGGTGCC TGCCATCTTC CGTTTGACAG AGGAGAATGG AACCTGGTAC 
TTGGACCAAA TCAGAAGAGA GCAGTATGTT CCAAACCAAG AATTTATTAA CTCAGATCTC 
CTTGAAAAGA ACAAATACCG AAAAATCTAT TCCTTTACTC TTGAGCCCCG AACTATTGAA 
GATTTTGAGT CCATGAATAC CTACCTTCAG ACATCACCAG CGTCTGTGTT TACTAGCAAA 
TCATTTTGTT CCCTGCAGAC CCCAGAAGGA GTTCATTGTT TGGTTGGCTC CACCCTCACT 
TATAGAAGAT TTAGTTACAA GGACAACGTC GATCTTGTAG AGTTTAAGAG TCTGACTGAG 
GAAGAAATAG AAGATGTACT GAGAACTATA TTTGGGGTTT CTTTAGAGAG AAAACTTGTG 
CCTAAACATG GTGATCGATT TTTTACCATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nat2 ( XM_006509592.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509655.1 Mus musculus N-acetyltransferase 2 (arylamine N-acetyltransferase) (Nat2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu40964
Clone ID Related Accession (Same CDS sequence)	XM_006509592.2
Accession Version	XM_006509592.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	arylamine N-acetyltransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006509592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAATAT TTCTGTTATT TCACTGCAGT GTCTGGAATT TGTTTCCAGG TGTGTCTCTT 
TCAGTCTCTT CCTCTGTGGA TCATCATTGT TTCACACTTT TCCCCCAATT CTGGGAAACC 
ATGGACATCG AAGCGTACTT TGAAAGAATT GGTTATCAGA GCACCAGGAG CAAACTGGAC 
TTGAAAACAT TAACCGAAAT CCTTCAGCAC CAGATACGGG CTATTCCCTT TGAGAATTTG 
AACATCCATT GTGGGGAATC CATGGAACTG AGTTTAGAAG CCATCTTTGA TCAAATTGTG 
AGGAAGAAGC GGGGTGGATG GTGTCTCCAG GTTAATCATC TGCTGTACTG GGCTCTGACC 
AAACTGGGCT TTGAAACCAC AATGCTGGGA GGATATGTCT TTAACACTCC AGCCAATAAG 
TACAGCAGTG GCATGATTCA CCTTCTAGTT CAGGTCACCA TCAGTGGAAA GGACTACATT 
GTTGATGCTG GGTTTGGACG TTCCTACCAG ATGTGGGAGC CTCTGGAATT AACATCTGGA 
AAGGATCAGC CTCAGGTGCC TGCCATCTTC CGTTTGACAG AGGAGAATGG AACCTGGTAC 
TTGGACCAAA TCAGAAGAGA GCAGTATGTT CCAAACCAAG AATTTATTAA CTCAGATCTC 
CTTGAAAAGA ACAAATACCG AAAAATCTAT TCCTTTACTC TTGAGCCCCG AACTATTGAA 
GATTTTGAGT CCATGAATAC CTACCTTCAG ACATCACCAG CGTCTGTGTT TACTAGCAAA 
TCATTTTGTT CCCTGCAGAC CCCAGAAGGA GTTCATTGTT TGGTTGGCTC CACCCTCACT 
TATAGAAGAT TTAGTTACAA GGACAACGTC GATCTTGTAG AGTTTAAGAG TCTGACTGAG 
GAAGAAATAG AAGATGTACT GAGAACTATA TTTGGGGTTT CTTTAGAGAG AAAACTTGTG 
CCTAAACATG GTGATCGATT TTTTACCATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509655.1
CDS	5600..6592
Translation	MPIFLLFHCSVWNLFPGVSLSVSSSVDHHCFTLFPQFWETMDIEAYFERIGYQSTRSKLDLKTLTEILQHQIRAIPFENLNIHCGESMELSLEAIFDQIVRKKRGGWCLQVNHLLYWALTKLGFETTMLGGYVFNTPANKYSSGMIHLLVQVTISGKDYIVDAGFGRSYQMWEPLELTSGKDQPQVPAIFRLTEENGTWYLDQIRREQYVPNQEFINSDLLEKNKYRKIYSFTLEPRTIEDFESMNTYLQTSPASVFTSKSFCSLQTPEGVHCLVGSTLTYRRFSYKDNVDLVEFKSLTEEEIEDVLRTIFGVSLERKLVPKHGDRFFTI

Target ORF information:

RefSeq Version	XM_006509592.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acetyltransferase 2 (arylamine N-acetyltransferase) (Nat2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509592.2

ORF Insert Sequence:

ATGCCAATAT TTCTGTTATT TCACTGCAGT GTCTGGAATT TGTTTCCAGG TGTGTCTCTT 
TCAGTCTCTT CCTCTGTGGA TCATCATTGT TTCACACTTT TCCCCCAATT CTGGGAAACC 
ATGGACATCG AAGCGTACTT TGAAAGAATT GGTTATCAGA GCACCAGGAG CAAACTGGAC 
TTGAAAACAT TAACCGAAAT CCTTCAGCAC CAGATACGGG CTATTCCCTT TGAGAATTTG 
AACATCCATT GTGGGGAATC CATGGAACTG AGTTTAGAAG CCATCTTTGA TCAAATTGTG 
AGGAAGAAGC GGGGTGGATG GTGTCTCCAG GTTAATCATC TGCTGTACTG GGCTCTGACC 
AAACTGGGCT TTGAAACCAC AATGCTGGGA GGATATGTCT TTAACACTCC AGCCAATAAG 
TACAGCAGTG GCATGATTCA CCTTCTAGTT CAGGTCACCA TCAGTGGAAA GGACTACATT 
GTTGATGCTG GGTTTGGACG TTCCTACCAG ATGTGGGAGC CTCTGGAATT AACATCTGGA 
AAGGATCAGC CTCAGGTGCC TGCCATCTTC CGTTTGACAG AGGAGAATGG AACCTGGTAC 
TTGGACCAAA TCAGAAGAGA GCAGTATGTT CCAAACCAAG AATTTATTAA CTCAGATCTC 
CTTGAAAAGA ACAAATACCG AAAAATCTAT TCCTTTACTC TTGAGCCCCG AACTATTGAA 
GATTTTGAGT CCATGAATAC CTACCTTCAG ACATCACCAG CGTCTGTGTT TACTAGCAAA 
TCATTTTGTT CCCTGCAGAC CCCAGAAGGA GTTCATTGTT TGGTTGGCTC CACCCTCACT 
TATAGAAGAT TTAGTTACAA GGACAACGTC GATCTTGTAG AGTTTAAGAG TCTGACTGAG 
GAAGAAATAG AAGATGTACT GAGAACTATA TTTGGGGTTT CTTTAGAGAG AAAACTTGTG 
CCTAAACATG GTGATCGATT TTTTACCATT TAG