Nf1 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Nf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu22792 NM_010897.2
Latest version!
Mus musculus neurofibromin 1 (Nf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45702 XM_006532436.4
Latest version!
Mus musculus neurofibromin 1 (Nf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45703 XM_006532437.4
Latest version!
Mus musculus neurofibromin 1 (Nf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45704 XM_006532438.4
Latest version!
Mus musculus neurofibromin 1 (Nf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45705 XM_006532439.4
Latest version!
Mus musculus neurofibromin 1 (Nf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45706 XM_006532440.4
Latest version!
Mus musculus neurofibromin 1 (Nf1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45707 XM_006532441.4
Latest version!
Mus musculus neurofibromin 1 (Nf1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45708 XM_006532442.4
Latest version!
Mus musculus neurofibromin 1 (Nf1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu85649 XM_030245644.1
Latest version!
Mus musculus neurofibromin 1 (Nf1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OMu45705 XM_006532439.3 Mus musculus neurofibromatosis 1 (Nf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45702 XM_006532436.3 Mus musculus neurofibromatosis 1 (Nf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45704 XM_006532438.3 Mus musculus neurofibromatosis 1 (Nf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45706 XM_006532440.3 Mus musculus neurofibromatosis 1 (Nf1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45707 XM_006532441.3 Mus musculus neurofibromatosis 1 (Nf1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45703 XM_006532437.3 Mus musculus neurofibromatosis 1 (Nf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OMu45708 XM_006532442.3 Mus musculus neurofibromatosis 1 (Nf1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu22792
    Clone ID Related Accession (Same CDS sequence) NM_010897.2
    Accession Version NM_010897.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Mus musculus(house mouse)
    Product neurofibromin
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591126.18 and AL591174.13. On Nov 17, 2006 this sequence version replaced NM_010897.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGC
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGCT GTGGAGCGCA CCCAGCAATA
    CGAATGGCAC CAAGTCTCAC CTTTAAAGAA AAGGTAACAA GCCTTAAATT TAAAGAAAAG
    CCTACAGACC TAGAGACAAG GAGCTACAAA TGTCTTCTTT TGTCCATGGT GAAACTGATT
    CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAGAGC
    AGTACAGCAG AACTAATTAC AGGGCTCGTA CAACTGGTTC CTCAGTCACA CATGCCAGAG
    GTCGCTCAGG AGGCTATGGA GGCTTTGCTG GTCCTTCATC AGTTAGATAG CATTGATCTC
    TGGAATCCTG ACGCTCCTGT AGAGACATTC TGGGAAATCA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA
    CGGGAAATTC TGATCTGCAG AAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGC
    TCCTGTCATT CTCTCTACCT TTATGGAGTA GGATGTGAAA TGTCTGCTAC TGGAAATACC
    ACTCAGATGT CAGTTGATCA TGATGAGTTC CTGCGTGCCT GTACTCCTGG AGCATCTCTG
    CGGAAAGGAA GAGGGAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCT
    ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCCTTAT ACATGTTTCT GTGGAATCCT
    GACACTGAAG CTGTTCTGGT TGCTATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT
    ATTCGATGTG GGGTAGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CCTCAGTCAG CAATATGATG TCAACAGGAA GAGCAGCGCT TCAGAAAAGA
    GTGATGGCCC TGCTAAGGCG CATTGAGCAC CCTACTGCAG GAAACATTGA GGCCTGGGAA
    GATACACATG CAAAGTGGGA ACAGGCTACA AAACTAATCC TTAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC AGAAAGTCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC
    CATGTCAGTG GAGGAGGCTC CATAGACTTG TCTGACACAG ACTCCCTGCA GGAGTGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT
    GGCCTAGCGA CATACAGCCC ACCTATGGGC GCTGTCAGTG AACGCAAAGG GTCTATGATT
    TCTGTAATGT CTTCTGAAGG GAATATTGAT TCACCTGTCA GCAGATTTAT GGACCGGCTT
    CTGTCCTTAA TGGTGTGTAA CCACGAGAAA GTGGGGCTTC AGATACGGAC CAATGTTAAG
    GACCTGGTGG GTCTGGAGTT GAGTCCTGCT CTGTATCCAA TGCTGTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TACTAAGTGA CAGCAATACT
    CAGTTTGTAG AGCAAACCAT AGCCATAATG AAAAACCTAC TGGATAATCA TACTGAAGGC
    AGCTCTGAGC ATCTGGGACA AGCGAGCATT GAGACAATGA TGCTAAATCT GGTCAGATAC
    GTTCGTGTTC TTGGGAATAT GGTCCATGCA ATACAAATAA AAACAAAATT GTGTCAGTTA
    GTTGAAGTCA TGATGGCAAG AAGAGATGAC CTCTCGTTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT AACAGACTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGACATAA AATGTCTTAC GAGAGATCTG GACCAAGCAA GCATGGAAGC AGTAGTTTCT
    CTCCTCGCTG GTCTTCCACT GCAGCCCGAA GAAGGAGATG GGGTGGAGCT GATGGAGGCC
    AAATCACAGT TATTCCTTAA GTACTTCACA TTATTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACGGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCCCTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC
    AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCACAAGG ATCTTCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTCGATAC ACTTGCGGAA
    ACGGTGTTGG CAGATCGATT TGAGAGACTT GTGGAACTGG TCACAATGAT GGGAGACCAG
    GGAGAACTCC CTATAGCTAT GGCTCTTGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG
    CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT TCCCGGCATT TACTCTACCA GCTGCTCTGG
    AACATGTTTT CTAAGGAGGT AGAATTGGCA GACTCCATGC AGACTCTCTT TCGAGGCAAC
    AGCTTGGCCA GTAAGATAAT GACATTCTGC TTCAAGGTGT ATGGTGCTAC TTACCTACAA
    AAGCTCTTGG ACCCTTTATT ACGAGTCATC ATCACATCTT CGGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACCC TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC
    CTCCTTCAGA TGACAGAGAA GTTCTTCCAT GCCATCATCA GCTCTTCCTC AGAATTCCCC
    TCACAGCTTC GAAGTGTCTG CCATTGTTTG TACCAGGCAA CTTGCCACTC CCTACTGAAT
    AAAGCTACAG TAAAAGAAAG AAAGGAAAAC AAAAAATCAG TGGTTAGCCA GCGCTTCCCT
    CAGAACAGCA TCGGTGCCGT AGGAAGTGCC ATGTTTCTCA GATTCATCAA TCCTGCCATT
    GTCTCGCCGT ATGAAGCAGG GATTTTAGAT AAAAAGCCAC CACCTAGAAT TGAAAGGGGC
    TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC ATTGCCAATC ATGTACTGTT CACAAAAGAA
    GAGCATATGA GACCTTTTAA TGATTTTGTG AAAAGCAACT TTGACTTGGC ACGAAGGTTT
    TTCCTAGATA TAGCATCAGA TTGTCCCACA AGTGATGCAG TAAACCATAG TCTTTCCTTC
    ATCAGCGATG GCAATGTGCT TGCTTTACAT CGGCTGCTTT GGAACAATCA GGAGAAAATT
    GGCCAGTATC TTTCCAGTAA CAGGGATCAT AAAGCTGTTG GAAGACGACC TTTTGATAAG
    ATGGCCACAC TTCTCGCGTA TCTGGGTCCT CCGGAGCACA AGCCTGTGGC AGATACACAC
    TGGTCCAGCC TTAACCTTAC CAGTTCAAAG TTTGAGGAAT TTATGACCAG GCACCAGGTA
    CATGAGAAAG AAGAGTTCAA GGCCTTGAAA ACGTTAAGCA TCTTCTACCA AGCTGGCACC
    TCCAAAGCTG GGAATCCTAT TTTTTATTAT GTTGCACGGA GGTTCAAAAC TGGCCAGATC
    AATGGTGATC TGCTGATATA CCATGTCTTG CTGACTTTAA AGCCATATTA TGCAAAGCCA
    TATGAAATTG TAGTGGACCT TACCCATACT GGGCCTAGCA ATCGCTTTAA AACAGACTTC
    CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC TTTGCCTATG ACAACGTCTC TGCAGTCTAT
    ATCTATAACT GTAACTCCTG GGTCAGGGAG TATACCAAAT ACCATGAGCG GCTGCTGACT
    GGCCTCAAGG GCAGCAAAAG GCTCATTTTC ATCGACTGTC CTGGGAAGCT GGCTGAACAT
    ATAGAGCATG AGCAACAGAA ACTCCCTGCT GCTACCTTGG CTTTGGAAGA AGATCTAAAG
    GTTTTCCACA ATGCTCTCAA GTTAGCTCAC AAGGACACCA AAGTTTCTAT TAAGGTTGGT
    TCTACTGCTG TTCAGGTAAC TTCAGCAGAA AGAACAAAAG TTTTGGGGCA ATCCGTCTTT
    TTAAATGATA TTTACTATGC TTCTGAAATC GAAGAGATCT GCCTAGTTGA TGAGAACCAG
    TTCACTCTAA CCATTGCAAA CCAAGGCACA CCACTGACCT TCATGCACCA AGAATGTGAA
    GCCATTGTTC AGTCTATCAT TCATATCAGG ACCCGCTGGG AGCTGTCACA ACCTGACTCC
    ATCCCTCAGC ATACCAAGAT TCGACCAAAG GATGTCCCTG GGACACTGCT TAATATTGCG
    TTACTGAATT TGGGCAGTTC AGACCCTAGT TTACGGTCTG CTGCCTATAA TCTCCTGTGT
    GCCTTAACCT GCACCTTTAA TTTAAAGATT GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA
    TGCATCCCTG CCAACAACAC ACTCTTCATT GTCTCTATCA GTAAGACGCT CGCAGCCAAT
    GAGCCACACC TCACCTTAGA GTTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAGT
    ATTGAATTGA AGCACCTTTG TTTGGAATAT ATGACCCCAT GGCTGTCAAA TCTAGTCCGT
    TTTTGTAAGC ATAATGATGA TGCCAAACGA CAAAGGGTTA CTGCCATCCT TGATAAGCTA
    ATAACAATGA CTATAAACGA GAAGCAGATG TATCCTTCTA TTCAAGCAAA AATCTGGGGG
    AGCCTTGGGC AGATCACAGA CCTGCTGGAT GTTGTGCTTG ACAGTTTCAT CAAGACCAGT
    GCGACAGGAG GCTTAGGGTC TATCAAAGCT GAGGTGATGG CAGACACAGC TGTGGCTTTA
    GCTTCTGGAA ATGTGAAATT GGTGTCGAGT AAGGTTATTG GAAGGATGTG TAAAATAATT
    GACAAGACTT GCTTATCCCC AACTCCAACT TTAGAACAAC ATCTTATGTG GGACGACATT
    GCCATTTTAG CCCGCTACAT GCTGATGCTG TCCTTCAACA ACTCCCTCGA TGTGGCGGCT
    CATCTGCCCT ATCTCTTCCA TGTTGTCACT TTCTTAGTAG CCACAGGTCC CTTGTCCCTC
    CGAGCTTCCA CACATGGGCT GCTCATCAAT ATCATTCACT CTCTGTGTAC TTGTTCTCAG
    CTTCACTTTA GTGAAGAGAC CAAGCAAGTT TTGAGGCTCA GTCTAACAGA GTTCTCGTTA
    CCCAAATTTT ACTTACTGTT TGGCATTAGC AAAGTCAAGT CGGCTGCTGT CATTGCCTTC
    CGTTCCAGTT ACCGGGACCG CTCCTTCTCC CCTGGCTCCT ATGAGAGGGA GACTTTTGCT
    TTGACGTCCC TGGAAACAGT CACAGAAGCT TTGTTGGAGA TCATGGAGGC ATGTATGAGA
    GATATTCCAA CATGCAAGTG GCTGGATCAG TGGACAGAAC TAGCTCAAAG ATTTGCGTTT
    CAGTATAACC CATCGCTGCA GCCAAGAGCT CTTGTGGTGT TTGGCTGTAT TAGCAAACGA
    GTGTCTCATG GGCAGATAAA GCAGATTATC CGAATTCTTA GCAAGGCACT TGAAAGTTGT
    TTAAAAGGAC CTGACACTTA CAACAGTCAA GTTCTGATAG AATCTACGGT GATAGCACTA
    ACAAAATTAC AGCCGCTTCT TAATAAGGAC TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT
    GCTGTGGCTG TGCTGCAGCT GGACGAAGTC AACTTGTATT CAGCCGGCAC TGCACTTCTG
    GAACAAAACC TGCACACCTT GGACAGTCTC CGGATATTCA ATGACAAGAG TCCGGAAGAA
    GTATTTATGG CGATCCGGAA TCCTCTGGAA TGGCACTGCA AGCAGATGGA TCACTTTGTC
    GGACTCAACT TCAACTCTAA CTTTAACTTT GCACTAGTTG GACACCTCTT AAAAGGGTAC
    AGGCATCCTT CACCTGCCAT TGTTGCAAGA ACAGTCAGAA TTTTGCATAC ACTACTAACT
    CTCGTTAACA AACATAGAAA TTGTGACAAA TTTGAGGTGA ATACACAGAG TGTGGCTTAC
    TTAGCAGCTC TACTCACAGT GTCTGAAGAG GTTCGAAGTC GCTGTAGCCT CAAGCATAGG
    AAGTCCCTTC TTCTTACTGA TATTTCAATG GAAAATGTTC CTATGGATAC ATATCCCATT
    CATCACGGTG ACCCCAGCTA TAGGACACTA AAGGAGACCC AGCCATGGTC CTCTCCCAAA
    GGTTCAGAAG GATACCTTGC AGCCACCTAC CCAGCTGTAG GCCAAACCAG TCCCCGAGCC
    AGGAAATCCA TGAGCTTGGA TATGGGACAG CCTTCTCAGG CCAACACAAA AAAATTGCTT
    GGAACAAGAA AAAGTTTTGA TCACTTGATA TCTGACACAA AGGCTCCTAA AAGACAAGAA
    ATGGAATCAG GGATTACAAC ACCCCCGAAA ATGAGAAGAG TGGCAGAGAC TGACTATGAA
    ATGGAAACCC AAAGGATTCC CTCCTCGCAG CAGCACCCAC ATTTACGGAA AGTCTCAGTC
    TCTGAATCAA ATGTTCTTTT GGATGAAGAA GTTCTTACCG ACCCAAAGAT CCAAGCGCTG
    CTTCTTACTG TTCTGGCTAC ACTGGTAAAA TATACTACAG ATGAATTTGA TCAACGGATT
    CTTTATGAAT ATCTAGCAGA GGCCAGCGTT GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC
    AATTTGTTGG ACTCTAAGAT CAATACCCTC TTGTCTTTAT GCCAAGATCC AAATTTGTTA
    AATCCAATCC ATGGAATTGT ACAGAGTGTG GTGTACCATG AGGAGTCCCC GCCACAGTAC
    CAGACATCTT ACCTGCAAAG TTTTGGTTTT AATGGCTTAT GGCGGTTTGC AGGACCCTTT
    TCAAAGCAAA CACAAATCCC TGACTATGCT GAGCTCATTG TTAAATTTCT TGATGCCTTG
    ATTGACACGT ACTTGCCTGG AATTGATGAA GAAACCAGTG AGGAGTCCCT CCTGACACCC
    ACATCTCCTT ACCCTCCTGC ACTGCAGAGC CAGCTTAGTA TCACTGCCAA CCTTAACCTC
    TCTAATTCCA TGACCTCACT TGCAACTTCC CAGCATTCCC CAGGACTCGA CAAGGAGAAC
    GTTGAACTCT CCCCCACCGC TGGCCACTGT AACAGTGGAC GGACTCGCCA TGGATCCGCA
    AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC AGTTTCAAAC GTAATAGCAT TAAGAAGATC
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_035027.1
    CDS109..8634
    Translation

    Target ORF information:

    RefSeq Version NM_010897.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromin 1 (Nf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_010897.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGC
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGCT GTGGAGCGCA CCCAGCAATA
    CGAATGGCAC CAAGTCTCAC CTTTAAAGAA AAGGTAACAA GCCTTAAATT TAAAGAAAAG
    CCTACAGACC TAGAGACAAG GAGCTACAAA TGTCTTCTTT TGTCCATGGT GAAACTGATT
    CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAGAGC
    AGTACAGCAG AACTAATTAC AGGGCTCGTA CAACTGGTTC CTCAGTCACA CATGCCAGAG
    GTCGCTCAGG AGGCTATGGA GGCTTTGCTG GTCCTTCATC AGTTAGATAG CATTGATCTC
    TGGAATCCTG ACGCTCCTGT AGAGACATTC TGGGAAATCA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA
    CGGGAAATTC TGATCTGCAG AAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGC
    TCCTGTCATT CTCTCTACCT TTATGGAGTA GGATGTGAAA TGTCTGCTAC TGGAAATACC
    ACTCAGATGT CAGTTGATCA TGATGAGTTC CTGCGTGCCT GTACTCCTGG AGCATCTCTG
    CGGAAAGGAA GAGGGAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCT
    ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCCTTAT ACATGTTTCT GTGGAATCCT
    GACACTGAAG CTGTTCTGGT TGCTATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT
    ATTCGATGTG GGGTAGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CCTCAGTCAG CAATATGATG TCAACAGGAA GAGCAGCGCT TCAGAAAAGA
    GTGATGGCCC TGCTAAGGCG CATTGAGCAC CCTACTGCAG GAAACATTGA GGCCTGGGAA
    GATACACATG CAAAGTGGGA ACAGGCTACA AAACTAATCC TTAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC AGAAAGTCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC
    CATGTCAGTG GAGGAGGCTC CATAGACTTG TCTGACACAG ACTCCCTGCA GGAGTGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT
    GGCCTAGCGA CATACAGCCC ACCTATGGGC GCTGTCAGTG AACGCAAAGG GTCTATGATT
    TCTGTAATGT CTTCTGAAGG GAATATTGAT TCACCTGTCA GCAGATTTAT GGACCGGCTT
    CTGTCCTTAA TGGTGTGTAA CCACGAGAAA GTGGGGCTTC AGATACGGAC CAATGTTAAG
    GACCTGGTGG GTCTGGAGTT GAGTCCTGCT CTGTATCCAA TGCTGTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TACTAAGTGA CAGCAATACT
    CAGTTTGTAG AGCAAACCAT AGCCATAATG AAAAACCTAC TGGATAATCA TACTGAAGGC
    AGCTCTGAGC ATCTGGGACA AGCGAGCATT GAGACAATGA TGCTAAATCT GGTCAGATAC
    GTTCGTGTTC TTGGGAATAT GGTCCATGCA ATACAAATAA AAACAAAATT GTGTCAGTTA
    GTTGAAGTCA TGATGGCAAG AAGAGATGAC CTCTCGTTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT AACAGACTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGACATAA AATGTCTTAC GAGAGATCTG GACCAAGCAA GCATGGAAGC AGTAGTTTCT
    CTCCTCGCTG GTCTTCCACT GCAGCCCGAA GAAGGAGATG GGGTGGAGCT GATGGAGGCC
    AAATCACAGT TATTCCTTAA GTACTTCACA TTATTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACGGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCCCTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC
    AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCACAAGG ATCTTCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTCGATAC ACTTGCGGAA
    ACGGTGTTGG CAGATCGATT TGAGAGACTT GTGGAACTGG TCACAATGAT GGGAGACCAG
    GGAGAACTCC CTATAGCTAT GGCTCTTGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG
    CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT TCCCGGCATT TACTCTACCA GCTGCTCTGG
    AACATGTTTT CTAAGGAGGT AGAATTGGCA GACTCCATGC AGACTCTCTT TCGAGGCAAC
    AGCTTGGCCA GTAAGATAAT GACATTCTGC TTCAAGGTGT ATGGTGCTAC TTACCTACAA
    AAGCTCTTGG ACCCTTTATT ACGAGTCATC ATCACATCTT CGGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACCC TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC
    CTCCTTCAGA TGACAGAGAA GTTCTTCCAT GCCATCATCA GCTCTTCCTC AGAATTCCCC
    TCACAGCTTC GAAGTGTCTG CCATTGTTTG TACCAGGCAA CTTGCCACTC CCTACTGAAT
    AAAGCTACAG TAAAAGAAAG AAAGGAAAAC AAAAAATCAG TGGTTAGCCA GCGCTTCCCT
    CAGAACAGCA TCGGTGCCGT AGGAAGTGCC ATGTTTCTCA GATTCATCAA TCCTGCCATT
    GTCTCGCCGT ATGAAGCAGG GATTTTAGAT AAAAAGCCAC CACCTAGAAT TGAAAGGGGC
    TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC ATTGCCAATC ATGTACTGTT CACAAAAGAA
    GAGCATATGA GACCTTTTAA TGATTTTGTG AAAAGCAACT TTGACTTGGC ACGAAGGTTT
    TTCCTAGATA TAGCATCAGA TTGTCCCACA AGTGATGCAG TAAACCATAG TCTTTCCTTC
    ATCAGCGATG GCAATGTGCT TGCTTTACAT CGGCTGCTTT GGAACAATCA GGAGAAAATT
    GGCCAGTATC TTTCCAGTAA CAGGGATCAT AAAGCTGTTG GAAGACGACC TTTTGATAAG
    ATGGCCACAC TTCTCGCGTA TCTGGGTCCT CCGGAGCACA AGCCTGTGGC AGATACACAC
    TGGTCCAGCC TTAACCTTAC CAGTTCAAAG TTTGAGGAAT TTATGACCAG GCACCAGGTA
    CATGAGAAAG AAGAGTTCAA GGCCTTGAAA ACGTTAAGCA TCTTCTACCA AGCTGGCACC
    TCCAAAGCTG GGAATCCTAT TTTTTATTAT GTTGCACGGA GGTTCAAAAC TGGCCAGATC
    AATGGTGATC TGCTGATATA CCATGTCTTG CTGACTTTAA AGCCATATTA TGCAAAGCCA
    TATGAAATTG TAGTGGACCT TACCCATACT GGGCCTAGCA ATCGCTTTAA AACAGACTTC
    CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC TTTGCCTATG ACAACGTCTC TGCAGTCTAT
    ATCTATAACT GTAACTCCTG GGTCAGGGAG TATACCAAAT ACCATGAGCG GCTGCTGACT
    GGCCTCAAGG GCAGCAAAAG GCTCATTTTC ATCGACTGTC CTGGGAAGCT GGCTGAACAT
    ATAGAGCATG AGCAACAGAA ACTCCCTGCT GCTACCTTGG CTTTGGAAGA AGATCTAAAG
    GTTTTCCACA ATGCTCTCAA GTTAGCTCAC AAGGACACCA AAGTTTCTAT TAAGGTTGGT
    TCTACTGCTG TTCAGGTAAC TTCAGCAGAA AGAACAAAAG TTTTGGGGCA ATCCGTCTTT
    TTAAATGATA TTTACTATGC TTCTGAAATC GAAGAGATCT GCCTAGTTGA TGAGAACCAG
    TTCACTCTAA CCATTGCAAA CCAAGGCACA CCACTGACCT TCATGCACCA AGAATGTGAA
    GCCATTGTTC AGTCTATCAT TCATATCAGG ACCCGCTGGG AGCTGTCACA ACCTGACTCC
    ATCCCTCAGC ATACCAAGAT TCGACCAAAG GATGTCCCTG GGACACTGCT TAATATTGCG
    TTACTGAATT TGGGCAGTTC AGACCCTAGT TTACGGTCTG CTGCCTATAA TCTCCTGTGT
    GCCTTAACCT GCACCTTTAA TTTAAAGATT GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA
    TGCATCCCTG CCAACAACAC ACTCTTCATT GTCTCTATCA GTAAGACGCT CGCAGCCAAT
    GAGCCACACC TCACCTTAGA GTTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAGT
    ATTGAATTGA AGCACCTTTG TTTGGAATAT ATGACCCCAT GGCTGTCAAA TCTAGTCCGT
    TTTTGTAAGC ATAATGATGA TGCCAAACGA CAAAGGGTTA CTGCCATCCT TGATAAGCTA
    ATAACAATGA CTATAAACGA GAAGCAGATG TATCCTTCTA TTCAAGCAAA AATCTGGGGG
    AGCCTTGGGC AGATCACAGA CCTGCTGGAT GTTGTGCTTG ACAGTTTCAT CAAGACCAGT
    GCGACAGGAG GCTTAGGGTC TATCAAAGCT GAGGTGATGG CAGACACAGC TGTGGCTTTA
    GCTTCTGGAA ATGTGAAATT GGTGTCGAGT AAGGTTATTG GAAGGATGTG TAAAATAATT
    GACAAGACTT GCTTATCCCC AACTCCAACT TTAGAACAAC ATCTTATGTG GGACGACATT
    GCCATTTTAG CCCGCTACAT GCTGATGCTG TCCTTCAACA ACTCCCTCGA TGTGGCGGCT
    CATCTGCCCT ATCTCTTCCA TGTTGTCACT TTCTTAGTAG CCACAGGTCC CTTGTCCCTC
    CGAGCTTCCA CACATGGGCT GCTCATCAAT ATCATTCACT CTCTGTGTAC TTGTTCTCAG
    CTTCACTTTA GTGAAGAGAC CAAGCAAGTT TTGAGGCTCA GTCTAACAGA GTTCTCGTTA
    CCCAAATTTT ACTTACTGTT TGGCATTAGC AAAGTCAAGT CGGCTGCTGT CATTGCCTTC
    CGTTCCAGTT ACCGGGACCG CTCCTTCTCC CCTGGCTCCT ATGAGAGGGA GACTTTTGCT
    TTGACGTCCC TGGAAACAGT CACAGAAGCT TTGTTGGAGA TCATGGAGGC ATGTATGAGA
    GATATTCCAA CATGCAAGTG GCTGGATCAG TGGACAGAAC TAGCTCAAAG ATTTGCGTTT
    CAGTATAACC CATCGCTGCA GCCAAGAGCT CTTGTGGTGT TTGGCTGTAT TAGCAAACGA
    GTGTCTCATG GGCAGATAAA GCAGATTATC CGAATTCTTA GCAAGGCACT TGAAAGTTGT
    TTAAAAGGAC CTGACACTTA CAACAGTCAA GTTCTGATAG AATCTACGGT GATAGCACTA
    ACAAAATTAC AGCCGCTTCT TAATAAGGAC TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT
    GCTGTGGCTG TGCTGCAGCT GGACGAAGTC AACTTGTATT CAGCCGGCAC TGCACTTCTG
    GAACAAAACC TGCACACCTT GGACAGTCTC CGGATATTCA ATGACAAGAG TCCGGAAGAA
    GTATTTATGG CGATCCGGAA TCCTCTGGAA TGGCACTGCA AGCAGATGGA TCACTTTGTC
    GGACTCAACT TCAACTCTAA CTTTAACTTT GCACTAGTTG GACACCTCTT AAAAGGGTAC
    AGGCATCCTT CACCTGCCAT TGTTGCAAGA ACAGTCAGAA TTTTGCATAC ACTACTAACT
    CTCGTTAACA AACATAGAAA TTGTGACAAA TTTGAGGTGA ATACACAGAG TGTGGCTTAC
    TTAGCAGCTC TACTCACAGT GTCTGAAGAG GTTCGAAGTC GCTGTAGCCT CAAGCATAGG
    AAGTCCCTTC TTCTTACTGA TATTTCAATG GAAAATGTTC CTATGGATAC ATATCCCATT
    CATCACGGTG ACCCCAGCTA TAGGACACTA AAGGAGACCC AGCCATGGTC CTCTCCCAAA
    GGTTCAGAAG GATACCTTGC AGCCACCTAC CCAGCTGTAG GCCAAACCAG TCCCCGAGCC
    AGGAAATCCA TGAGCTTGGA TATGGGACAG CCTTCTCAGG CCAACACAAA AAAATTGCTT
    GGAACAAGAA AAAGTTTTGA TCACTTGATA TCTGACACAA AGGCTCCTAA AAGACAAGAA
    ATGGAATCAG GGATTACAAC ACCCCCGAAA ATGAGAAGAG TGGCAGAGAC TGACTATGAA
    ATGGAAACCC AAAGGATTCC CTCCTCGCAG CAGCACCCAC ATTTACGGAA AGTCTCAGTC
    TCTGAATCAA ATGTTCTTTT GGATGAAGAA GTTCTTACCG ACCCAAAGAT CCAAGCGCTG
    CTTCTTACTG TTCTGGCTAC ACTGGTAAAA TATACTACAG ATGAATTTGA TCAACGGATT
    CTTTATGAAT ATCTAGCAGA GGCCAGCGTT GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC
    AATTTGTTGG ACTCTAAGAT CAATACCCTC TTGTCTTTAT GCCAAGATCC AAATTTGTTA
    AATCCAATCC ATGGAATTGT ACAGAGTGTG GTGTACCATG AGGAGTCCCC GCCACAGTAC
    CAGACATCTT ACCTGCAAAG TTTTGGTTTT AATGGCTTAT GGCGGTTTGC AGGACCCTTT
    TCAAAGCAAA CACAAATCCC TGACTATGCT GAGCTCATTG TTAAATTTCT TGATGCCTTG
    ATTGACACGT ACTTGCCTGG AATTGATGAA GAAACCAGTG AGGAGTCCCT CCTGACACCC
    ACATCTCCTT ACCCTCCTGC ACTGCAGAGC CAGCTTAGTA TCACTGCCAA CCTTAACCTC
    TCTAATTCCA TGACCTCACT TGCAACTTCC CAGCATTCCC CAGGACTCGA CAAGGAGAAC
    GTTGAACTCT CCCCCACCGC TGGCCACTGT AACAGTGGAC GGACTCGCCA TGGATCCGCA
    AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC AGTTTCAAAC GTAATAGCAT TAAGAAGATC
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45702
    Clone ID Related Accession (Same CDS sequence) XM_006532436.4 , XM_006532436.3
    Accession Version XM_006532436.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532436.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAG AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGCTTCCCT CAGAACAGCA TCGGTGCCGT AGGAAGTGCC
    ATGTTTCTCA GATTCATCAA TCCTGCCATT GTCTCGCCGT ATGAAGCAGG GATTTTAGAT
    AAAAAGCCAC CACCTAGAAT TGAAAGGGGC TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC
    ATTGCCAATC ATGTACTGTT CACAAAAGAA GAGCATATGA GACCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGACTTGGC ACGAAGGTTT TTCCTAGATA TAGCATCAGA TTGTCCCACA
    AGTGATGCAG TAAACCATAG TCTTTCCTTC ATCAGCGATG GCAATGTGCT TGCTTTACAT
    CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGTAA CAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCACAC TTCTCGCGTA TCTGGGTCCT
    CCGGAGCACA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTTGAGGAAT TTATGACCAG GCACCAGGTA CATGAGAAAG AAGAGTTCAA GGCCTTGAAA
    ACGTTAAGCA TCTTCTACCA AGCTGGCACC TCCAAAGCTG GGAATCCTAT TTTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGCCAGATC AATGGTGATC TGCTGATATA CCATGTCTTG
    CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT
    GGGCCTAGCA ATCGCTTTAA AACAGACTTC CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC
    TTTGCCTATG ACAACGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TATACCAAAT ACCATGAGCG GCTGCTGACT GGCCTCAAGG GCAGCAAAAG GCTCATTTTC
    ATCGACTGTC CTGGGAAGCT GGCTGAACAT ATAGAGCATG AGCAACAGAA ACTCCCTGCT
    GCTACCTTGG CTTTGGAAGA AGATCTAAAG GTTTTCCACA ATGCTCTCAA GTTAGCTCAC
    AAGGACACCA AAGTTTCTAT TAAGGTTGGT TCTACTGCTG TTCAGGTAAC TTCAGCAGAA
    AGAACAAAAG TTTTGGGGCA ATCCGTCTTT TTAAATGATA TTTACTATGC TTCTGAAATC
    GAAGAGATCT GCCTAGTTGA TGAGAACCAG TTCACTCTAA CCATTGCAAA CCAAGGCACA
    CCACTGACCT TCATGCACCA AGAATGTGAA GCCATTGTTC AGTCTATCAT TCATATCAGG
    ACCCGCTGGG AGCTGTCACA ACCTGACTCC ATCCCTCAGC ATACCAAGAT TCGACCAAAG
    GATGTCCCTG GGACACTGCT TAATATTGCG TTACTGAATT TGGGCAGTTC AGACCCTAGT
    TTACGGTCTG CTGCCTATAA TCTCCTGTGT GCCTTAACCT GCACCTTTAA TTTAAAGATT
    GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA TGCATCCCTG CCAACAACAC ACTCTTCATT
    GTCTCTATCA GTAAGACGCT CGCAGCCAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT
    ATGACCCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ATAATGATGA TGCCAAACGA
    CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CTATAAACGA GAAGCAGATG
    TATCCTTCTA TTCAAGCAAA AATCTGGGGG AGCCTTGGGC AGATCACAGA CCTGCTGGAT
    GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCGACAGGAG GCTTAGGGTC TATCAAAGCT
    GAGGTGATGG CAGACACAGC TGTGGCTTTA GCTTCTGGAA ATGTGAAATT GGTGTCGAGT
    AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCCCC AACTCCAACT
    TTAGAACAAC ATCTTATGTG GGACGACATT GCCATTTTAG CCCGCTACAT GCTGATGCTG
    TCCTTCAACA ACTCCCTCGA TGTGGCGGCT CATCTGCCCT ATCTCTTCCA TGTTGTCACT
    TTCTTAGTAG CCACAGGTCC CTTGTCCCTC CGAGCTTCCA CACATGGGCT GCTCATCAAT
    ATCATTCACT CTCTGTGTAC TTGTTCTCAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT
    TTGAGGCTCA GTCTAACAGA GTTCTCGTTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CGGCTGCTGT CATTGCCTTC CGTTCCAGTT ACCGGGACCG CTCCTTCTCC
    CCTGGCTCCT ATGAGAGGGA GACTTTTGCT TTGACGTCCC TGGAAACAGT CACAGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG ATTTGCGTTT CAGTATAACC CATCGCTGCA GCCAAGAGCT
    CTTGTGGTGT TTGGCTGTAT TAGCAAACGA GTGTCTCATG GGCAGATAAA GCAGATTATC
    CGAATTCTTA GCAAGGCACT TGAAAGTTGT TTAAAAGGAC CTGACACTTA CAACAGTCAA
    GTTCTGATAG AATCTACGGT GATAGCACTA ACAAAATTAC AGCCGCTTCT TAATAAGGAC
    TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT GCTGTGGCTG TGCTGCAGCT GGACGAAGTC
    AACTTGTATT CAGCCGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT GGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCGGAAGAA GTATTTATGG CGATCCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAGATGGA TCACTTTGTC GGACTCAACT TCAACTCTAA CTTTAACTTT
    GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTCGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG
    GTTCGAAGTC GCTGTAGCCT CAAGCATAGG AAGTCCCTTC TTCTTACTGA TATTTCAATG
    GAAAATGTTC CTATGGATAC ATATCCCATT CATCACGGTG ACCCCAGCTA TAGGACACTA
    AAGGAGACCC AGCCATGGTC CTCTCCCAAA GGTTCAGAAG GATACCTTGC AGCCACCTAC
    CCAGCTGTAG GCCAAACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA TATGGGACAG
    CCTTCTCAGG CCAACACAAA AAAATTGCTT GGAACAAGAA AAAGTTTTGA TCACTTGATA
    TCTGACACAA AGGCTCCTAA AAGACAAGAA ATGGAATCAG GGATTACAAC ACCCCCGAAA
    ATGAGAAGAG TGGCAGAGAC TGACTATGAA ATGGCAGAAA CCCAAAGGAT TCCCTCCTCG
    CAGCAGCACC CACATTTACG GAAAGTCTCA GTCTCTGAAT CAAATGTTCT TTTGGATGAA
    GAAGTTCTTA CCGACCCAAA GATCCAAGCG CTGCTTCTTA CTGTTCTGGC TACACTGGTA
    AAATATACTA CAGATGAATT TGATCAACGG ATTCTTTATG AATATCTAGC AGAGGCCAGC
    GTTGTGTTTC CCAAAGTGTT TCCTGTTGTG CACAATTTGT TGGACTCTAA GATCAATACC
    CTCTTGTCTT TATGCCAAGA TCCAAATTTG TTAAATCCAA TCCATGGAAT TGTACAGAGT
    GTGGTGTACC ATGAGGAGTC CCCGCCACAG TACCAGACAT CTTACCTGCA AAGTTTTGGT
    TTTAATGGCT TATGGCGGTT TGCAGGACCC TTTTCAAAGC AAACACAAAT CCCTGACTAT
    GCTGAGCTCA TTGTTAAATT TCTTGATGCC TTGATTGACA CGTACTTGCC TGGAATTGAT
    GAAGAAACCA GTGAGGAGTC CCTCCTGACA CCCACATCTC CTTACCCTCC TGCACTGCAG
    AGCCAGCTTA GTATCACTGC CAACCTTAAC CTCTCTAATT CCATGACCTC ACTTGCAACT
    TCCCAGCATT CCCCAGGACT CGACAAGGAG AACGTTGAAC TCTCCCCCAC CGCTGGCCAC
    TGTAACAGTG GACGGACTCG CCATGGATCC GCAAGCCAAG TGCAGAAGCA AAGAAGTGCT
    GGCAGTTTCA AACGTAATAG CATTAAGAAG ATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532499.1
    CDS407..8965
    Translation

    Target ORF information:

    RefSeq Version XM_006532436.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromin 1 (Nf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532436.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAG AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGCTTCCCT CAGAACAGCA TCGGTGCCGT AGGAAGTGCC
    ATGTTTCTCA GATTCATCAA TCCTGCCATT GTCTCGCCGT ATGAAGCAGG GATTTTAGAT
    AAAAAGCCAC CACCTAGAAT TGAAAGGGGC TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC
    ATTGCCAATC ATGTACTGTT CACAAAAGAA GAGCATATGA GACCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGACTTGGC ACGAAGGTTT TTCCTAGATA TAGCATCAGA TTGTCCCACA
    AGTGATGCAG TAAACCATAG TCTTTCCTTC ATCAGCGATG GCAATGTGCT TGCTTTACAT
    CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGTAA CAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCACAC TTCTCGCGTA TCTGGGTCCT
    CCGGAGCACA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTTGAGGAAT TTATGACCAG GCACCAGGTA CATGAGAAAG AAGAGTTCAA GGCCTTGAAA
    ACGTTAAGCA TCTTCTACCA AGCTGGCACC TCCAAAGCTG GGAATCCTAT TTTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGCCAGATC AATGGTGATC TGCTGATATA CCATGTCTTG
    CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT
    GGGCCTAGCA ATCGCTTTAA AACAGACTTC CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC
    TTTGCCTATG ACAACGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TATACCAAAT ACCATGAGCG GCTGCTGACT GGCCTCAAGG GCAGCAAAAG GCTCATTTTC
    ATCGACTGTC CTGGGAAGCT GGCTGAACAT ATAGAGCATG AGCAACAGAA ACTCCCTGCT
    GCTACCTTGG CTTTGGAAGA AGATCTAAAG GTTTTCCACA ATGCTCTCAA GTTAGCTCAC
    AAGGACACCA AAGTTTCTAT TAAGGTTGGT TCTACTGCTG TTCAGGTAAC TTCAGCAGAA
    AGAACAAAAG TTTTGGGGCA ATCCGTCTTT TTAAATGATA TTTACTATGC TTCTGAAATC
    GAAGAGATCT GCCTAGTTGA TGAGAACCAG TTCACTCTAA CCATTGCAAA CCAAGGCACA
    CCACTGACCT TCATGCACCA AGAATGTGAA GCCATTGTTC AGTCTATCAT TCATATCAGG
    ACCCGCTGGG AGCTGTCACA ACCTGACTCC ATCCCTCAGC ATACCAAGAT TCGACCAAAG
    GATGTCCCTG GGACACTGCT TAATATTGCG TTACTGAATT TGGGCAGTTC AGACCCTAGT
    TTACGGTCTG CTGCCTATAA TCTCCTGTGT GCCTTAACCT GCACCTTTAA TTTAAAGATT
    GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA TGCATCCCTG CCAACAACAC ACTCTTCATT
    GTCTCTATCA GTAAGACGCT CGCAGCCAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT
    ATGACCCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ATAATGATGA TGCCAAACGA
    CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CTATAAACGA GAAGCAGATG
    TATCCTTCTA TTCAAGCAAA AATCTGGGGG AGCCTTGGGC AGATCACAGA CCTGCTGGAT
    GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCGACAGGAG GCTTAGGGTC TATCAAAGCT
    GAGGTGATGG CAGACACAGC TGTGGCTTTA GCTTCTGGAA ATGTGAAATT GGTGTCGAGT
    AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCCCC AACTCCAACT
    TTAGAACAAC ATCTTATGTG GGACGACATT GCCATTTTAG CCCGCTACAT GCTGATGCTG
    TCCTTCAACA ACTCCCTCGA TGTGGCGGCT CATCTGCCCT ATCTCTTCCA TGTTGTCACT
    TTCTTAGTAG CCACAGGTCC CTTGTCCCTC CGAGCTTCCA CACATGGGCT GCTCATCAAT
    ATCATTCACT CTCTGTGTAC TTGTTCTCAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT
    TTGAGGCTCA GTCTAACAGA GTTCTCGTTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CGGCTGCTGT CATTGCCTTC CGTTCCAGTT ACCGGGACCG CTCCTTCTCC
    CCTGGCTCCT ATGAGAGGGA GACTTTTGCT TTGACGTCCC TGGAAACAGT CACAGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG ATTTGCGTTT CAGTATAACC CATCGCTGCA GCCAAGAGCT
    CTTGTGGTGT TTGGCTGTAT TAGCAAACGA GTGTCTCATG GGCAGATAAA GCAGATTATC
    CGAATTCTTA GCAAGGCACT TGAAAGTTGT TTAAAAGGAC CTGACACTTA CAACAGTCAA
    GTTCTGATAG AATCTACGGT GATAGCACTA ACAAAATTAC AGCCGCTTCT TAATAAGGAC
    TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT GCTGTGGCTG TGCTGCAGCT GGACGAAGTC
    AACTTGTATT CAGCCGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT GGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCGGAAGAA GTATTTATGG CGATCCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAGATGGA TCACTTTGTC GGACTCAACT TCAACTCTAA CTTTAACTTT
    GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTCGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG
    GTTCGAAGTC GCTGTAGCCT CAAGCATAGG AAGTCCCTTC TTCTTACTGA TATTTCAATG
    GAAAATGTTC CTATGGATAC ATATCCCATT CATCACGGTG ACCCCAGCTA TAGGACACTA
    AAGGAGACCC AGCCATGGTC CTCTCCCAAA GGTTCAGAAG GATACCTTGC AGCCACCTAC
    CCAGCTGTAG GCCAAACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA TATGGGACAG
    CCTTCTCAGG CCAACACAAA AAAATTGCTT GGAACAAGAA AAAGTTTTGA TCACTTGATA
    TCTGACACAA AGGCTCCTAA AAGACAAGAA ATGGAATCAG GGATTACAAC ACCCCCGAAA
    ATGAGAAGAG TGGCAGAGAC TGACTATGAA ATGGCAGAAA CCCAAAGGAT TCCCTCCTCG
    CAGCAGCACC CACATTTACG GAAAGTCTCA GTCTCTGAAT CAAATGTTCT TTTGGATGAA
    GAAGTTCTTA CCGACCCAAA GATCCAAGCG CTGCTTCTTA CTGTTCTGGC TACACTGGTA
    AAATATACTA CAGATGAATT TGATCAACGG ATTCTTTATG AATATCTAGC AGAGGCCAGC
    GTTGTGTTTC CCAAAGTGTT TCCTGTTGTG CACAATTTGT TGGACTCTAA GATCAATACC
    CTCTTGTCTT TATGCCAAGA TCCAAATTTG TTAAATCCAA TCCATGGAAT TGTACAGAGT
    GTGGTGTACC ATGAGGAGTC CCCGCCACAG TACCAGACAT CTTACCTGCA AAGTTTTGGT
    TTTAATGGCT TATGGCGGTT TGCAGGACCC TTTTCAAAGC AAACACAAAT CCCTGACTAT
    GCTGAGCTCA TTGTTAAATT TCTTGATGCC TTGATTGACA CGTACTTGCC TGGAATTGAT
    GAAGAAACCA GTGAGGAGTC CCTCCTGACA CCCACATCTC CTTACCCTCC TGCACTGCAG
    AGCCAGCTTA GTATCACTGC CAACCTTAAC CTCTCTAATT CCATGACCTC ACTTGCAACT
    TCCCAGCATT CCCCAGGACT CGACAAGGAG AACGTTGAAC TCTCCCCCAC CGCTGGCCAC
    TGTAACAGTG GACGGACTCG CCATGGATCC GCAAGCCAAG TGCAGAAGCA AAGAAGTGCT
    GGCAGTTTCA AACGTAATAG CATTAAGAAG ATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45702
    Clone ID Related Accession (Same CDS sequence) XM_006532436.4 , XM_006532436.3
    Accession Version XM_006532436.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532436.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAG AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGCTTCCCT CAGAACAGCA TCGGTGCCGT AGGAAGTGCC
    ATGTTTCTCA GATTCATCAA TCCTGCCATT GTCTCGCCGT ATGAAGCAGG GATTTTAGAT
    AAAAAGCCAC CACCTAGAAT TGAAAGGGGC TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC
    ATTGCCAATC ATGTACTGTT CACAAAAGAA GAGCATATGA GACCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGACTTGGC ACGAAGGTTT TTCCTAGATA TAGCATCAGA TTGTCCCACA
    AGTGATGCAG TAAACCATAG TCTTTCCTTC ATCAGCGATG GCAATGTGCT TGCTTTACAT
    CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGTAA CAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCACAC TTCTCGCGTA TCTGGGTCCT
    CCGGAGCACA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTTGAGGAAT TTATGACCAG GCACCAGGTA CATGAGAAAG AAGAGTTCAA GGCCTTGAAA
    ACGTTAAGCA TCTTCTACCA AGCTGGCACC TCCAAAGCTG GGAATCCTAT TTTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGCCAGATC AATGGTGATC TGCTGATATA CCATGTCTTG
    CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT
    GGGCCTAGCA ATCGCTTTAA AACAGACTTC CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC
    TTTGCCTATG ACAACGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TATACCAAAT ACCATGAGCG GCTGCTGACT GGCCTCAAGG GCAGCAAAAG GCTCATTTTC
    ATCGACTGTC CTGGGAAGCT GGCTGAACAT ATAGAGCATG AGCAACAGAA ACTCCCTGCT
    GCTACCTTGG CTTTGGAAGA AGATCTAAAG GTTTTCCACA ATGCTCTCAA GTTAGCTCAC
    AAGGACACCA AAGTTTCTAT TAAGGTTGGT TCTACTGCTG TTCAGGTAAC TTCAGCAGAA
    AGAACAAAAG TTTTGGGGCA ATCCGTCTTT TTAAATGATA TTTACTATGC TTCTGAAATC
    GAAGAGATCT GCCTAGTTGA TGAGAACCAG TTCACTCTAA CCATTGCAAA CCAAGGCACA
    CCACTGACCT TCATGCACCA AGAATGTGAA GCCATTGTTC AGTCTATCAT TCATATCAGG
    ACCCGCTGGG AGCTGTCACA ACCTGACTCC ATCCCTCAGC ATACCAAGAT TCGACCAAAG
    GATGTCCCTG GGACACTGCT TAATATTGCG TTACTGAATT TGGGCAGTTC AGACCCTAGT
    TTACGGTCTG CTGCCTATAA TCTCCTGTGT GCCTTAACCT GCACCTTTAA TTTAAAGATT
    GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA TGCATCCCTG CCAACAACAC ACTCTTCATT
    GTCTCTATCA GTAAGACGCT CGCAGCCAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT
    ATGACCCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ATAATGATGA TGCCAAACGA
    CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CTATAAACGA GAAGCAGATG
    TATCCTTCTA TTCAAGCAAA AATCTGGGGG AGCCTTGGGC AGATCACAGA CCTGCTGGAT
    GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCGACAGGAG GCTTAGGGTC TATCAAAGCT
    GAGGTGATGG CAGACACAGC TGTGGCTTTA GCTTCTGGAA ATGTGAAATT GGTGTCGAGT
    AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCCCC AACTCCAACT
    TTAGAACAAC ATCTTATGTG GGACGACATT GCCATTTTAG CCCGCTACAT GCTGATGCTG
    TCCTTCAACA ACTCCCTCGA TGTGGCGGCT CATCTGCCCT ATCTCTTCCA TGTTGTCACT
    TTCTTAGTAG CCACAGGTCC CTTGTCCCTC CGAGCTTCCA CACATGGGCT GCTCATCAAT
    ATCATTCACT CTCTGTGTAC TTGTTCTCAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT
    TTGAGGCTCA GTCTAACAGA GTTCTCGTTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CGGCTGCTGT CATTGCCTTC CGTTCCAGTT ACCGGGACCG CTCCTTCTCC
    CCTGGCTCCT ATGAGAGGGA GACTTTTGCT TTGACGTCCC TGGAAACAGT CACAGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG ATTTGCGTTT CAGTATAACC CATCGCTGCA GCCAAGAGCT
    CTTGTGGTGT TTGGCTGTAT TAGCAAACGA GTGTCTCATG GGCAGATAAA GCAGATTATC
    CGAATTCTTA GCAAGGCACT TGAAAGTTGT TTAAAAGGAC CTGACACTTA CAACAGTCAA
    GTTCTGATAG AATCTACGGT GATAGCACTA ACAAAATTAC AGCCGCTTCT TAATAAGGAC
    TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT GCTGTGGCTG TGCTGCAGCT GGACGAAGTC
    AACTTGTATT CAGCCGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT GGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCGGAAGAA GTATTTATGG CGATCCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAGATGGA TCACTTTGTC GGACTCAACT TCAACTCTAA CTTTAACTTT
    GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTCGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG
    GTTCGAAGTC GCTGTAGCCT CAAGCATAGG AAGTCCCTTC TTCTTACTGA TATTTCAATG
    GAAAATGTTC CTATGGATAC ATATCCCATT CATCACGGTG ACCCCAGCTA TAGGACACTA
    AAGGAGACCC AGCCATGGTC CTCTCCCAAA GGTTCAGAAG GATACCTTGC AGCCACCTAC
    CCAGCTGTAG GCCAAACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA TATGGGACAG
    CCTTCTCAGG CCAACACAAA AAAATTGCTT GGAACAAGAA AAAGTTTTGA TCACTTGATA
    TCTGACACAA AGGCTCCTAA AAGACAAGAA ATGGAATCAG GGATTACAAC ACCCCCGAAA
    ATGAGAAGAG TGGCAGAGAC TGACTATGAA ATGGCAGAAA CCCAAAGGAT TCCCTCCTCG
    CAGCAGCACC CACATTTACG GAAAGTCTCA GTCTCTGAAT CAAATGTTCT TTTGGATGAA
    GAAGTTCTTA CCGACCCAAA GATCCAAGCG CTGCTTCTTA CTGTTCTGGC TACACTGGTA
    AAATATACTA CAGATGAATT TGATCAACGG ATTCTTTATG AATATCTAGC AGAGGCCAGC
    GTTGTGTTTC CCAAAGTGTT TCCTGTTGTG CACAATTTGT TGGACTCTAA GATCAATACC
    CTCTTGTCTT TATGCCAAGA TCCAAATTTG TTAAATCCAA TCCATGGAAT TGTACAGAGT
    GTGGTGTACC ATGAGGAGTC CCCGCCACAG TACCAGACAT CTTACCTGCA AAGTTTTGGT
    TTTAATGGCT TATGGCGGTT TGCAGGACCC TTTTCAAAGC AAACACAAAT CCCTGACTAT
    GCTGAGCTCA TTGTTAAATT TCTTGATGCC TTGATTGACA CGTACTTGCC TGGAATTGAT
    GAAGAAACCA GTGAGGAGTC CCTCCTGACA CCCACATCTC CTTACCCTCC TGCACTGCAG
    AGCCAGCTTA GTATCACTGC CAACCTTAAC CTCTCTAATT CCATGACCTC ACTTGCAACT
    TCCCAGCATT CCCCAGGACT CGACAAGGAG AACGTTGAAC TCTCCCCCAC CGCTGGCCAC
    TGTAACAGTG GACGGACTCG CCATGGATCC GCAAGCCAAG TGCAGAAGCA AAGAAGTGCT
    GGCAGTTTCA AACGTAATAG CATTAAGAAG ATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532499.1
    CDS1612..10170
    Translation

    Target ORF information:

    RefSeq Version XM_006532436.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromatosis 1 (Nf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532436.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAG AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGCTTCCCT CAGAACAGCA TCGGTGCCGT AGGAAGTGCC
    ATGTTTCTCA GATTCATCAA TCCTGCCATT GTCTCGCCGT ATGAAGCAGG GATTTTAGAT
    AAAAAGCCAC CACCTAGAAT TGAAAGGGGC TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC
    ATTGCCAATC ATGTACTGTT CACAAAAGAA GAGCATATGA GACCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGACTTGGC ACGAAGGTTT TTCCTAGATA TAGCATCAGA TTGTCCCACA
    AGTGATGCAG TAAACCATAG TCTTTCCTTC ATCAGCGATG GCAATGTGCT TGCTTTACAT
    CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGTAA CAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCACAC TTCTCGCGTA TCTGGGTCCT
    CCGGAGCACA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTTGAGGAAT TTATGACCAG GCACCAGGTA CATGAGAAAG AAGAGTTCAA GGCCTTGAAA
    ACGTTAAGCA TCTTCTACCA AGCTGGCACC TCCAAAGCTG GGAATCCTAT TTTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGCCAGATC AATGGTGATC TGCTGATATA CCATGTCTTG
    CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT
    GGGCCTAGCA ATCGCTTTAA AACAGACTTC CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC
    TTTGCCTATG ACAACGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TATACCAAAT ACCATGAGCG GCTGCTGACT GGCCTCAAGG GCAGCAAAAG GCTCATTTTC
    ATCGACTGTC CTGGGAAGCT GGCTGAACAT ATAGAGCATG AGCAACAGAA ACTCCCTGCT
    GCTACCTTGG CTTTGGAAGA AGATCTAAAG GTTTTCCACA ATGCTCTCAA GTTAGCTCAC
    AAGGACACCA AAGTTTCTAT TAAGGTTGGT TCTACTGCTG TTCAGGTAAC TTCAGCAGAA
    AGAACAAAAG TTTTGGGGCA ATCCGTCTTT TTAAATGATA TTTACTATGC TTCTGAAATC
    GAAGAGATCT GCCTAGTTGA TGAGAACCAG TTCACTCTAA CCATTGCAAA CCAAGGCACA
    CCACTGACCT TCATGCACCA AGAATGTGAA GCCATTGTTC AGTCTATCAT TCATATCAGG
    ACCCGCTGGG AGCTGTCACA ACCTGACTCC ATCCCTCAGC ATACCAAGAT TCGACCAAAG
    GATGTCCCTG GGACACTGCT TAATATTGCG TTACTGAATT TGGGCAGTTC AGACCCTAGT
    TTACGGTCTG CTGCCTATAA TCTCCTGTGT GCCTTAACCT GCACCTTTAA TTTAAAGATT
    GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA TGCATCCCTG CCAACAACAC ACTCTTCATT
    GTCTCTATCA GTAAGACGCT CGCAGCCAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT
    ATGACCCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ATAATGATGA TGCCAAACGA
    CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CTATAAACGA GAAGCAGATG
    TATCCTTCTA TTCAAGCAAA AATCTGGGGG AGCCTTGGGC AGATCACAGA CCTGCTGGAT
    GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCGACAGGAG GCTTAGGGTC TATCAAAGCT
    GAGGTGATGG CAGACACAGC TGTGGCTTTA GCTTCTGGAA ATGTGAAATT GGTGTCGAGT
    AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCCCC AACTCCAACT
    TTAGAACAAC ATCTTATGTG GGACGACATT GCCATTTTAG CCCGCTACAT GCTGATGCTG
    TCCTTCAACA ACTCCCTCGA TGTGGCGGCT CATCTGCCCT ATCTCTTCCA TGTTGTCACT
    TTCTTAGTAG CCACAGGTCC CTTGTCCCTC CGAGCTTCCA CACATGGGCT GCTCATCAAT
    ATCATTCACT CTCTGTGTAC TTGTTCTCAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT
    TTGAGGCTCA GTCTAACAGA GTTCTCGTTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CGGCTGCTGT CATTGCCTTC CGTTCCAGTT ACCGGGACCG CTCCTTCTCC
    CCTGGCTCCT ATGAGAGGGA GACTTTTGCT TTGACGTCCC TGGAAACAGT CACAGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG ATTTGCGTTT CAGTATAACC CATCGCTGCA GCCAAGAGCT
    CTTGTGGTGT TTGGCTGTAT TAGCAAACGA GTGTCTCATG GGCAGATAAA GCAGATTATC
    CGAATTCTTA GCAAGGCACT TGAAAGTTGT TTAAAAGGAC CTGACACTTA CAACAGTCAA
    GTTCTGATAG AATCTACGGT GATAGCACTA ACAAAATTAC AGCCGCTTCT TAATAAGGAC
    TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT GCTGTGGCTG TGCTGCAGCT GGACGAAGTC
    AACTTGTATT CAGCCGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT GGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCGGAAGAA GTATTTATGG CGATCCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAGATGGA TCACTTTGTC GGACTCAACT TCAACTCTAA CTTTAACTTT
    GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTCGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG
    GTTCGAAGTC GCTGTAGCCT CAAGCATAGG AAGTCCCTTC TTCTTACTGA TATTTCAATG
    GAAAATGTTC CTATGGATAC ATATCCCATT CATCACGGTG ACCCCAGCTA TAGGACACTA
    AAGGAGACCC AGCCATGGTC CTCTCCCAAA GGTTCAGAAG GATACCTTGC AGCCACCTAC
    CCAGCTGTAG GCCAAACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA TATGGGACAG
    CCTTCTCAGG CCAACACAAA AAAATTGCTT GGAACAAGAA AAAGTTTTGA TCACTTGATA
    TCTGACACAA AGGCTCCTAA AAGACAAGAA ATGGAATCAG GGATTACAAC ACCCCCGAAA
    ATGAGAAGAG TGGCAGAGAC TGACTATGAA ATGGCAGAAA CCCAAAGGAT TCCCTCCTCG
    CAGCAGCACC CACATTTACG GAAAGTCTCA GTCTCTGAAT CAAATGTTCT TTTGGATGAA
    GAAGTTCTTA CCGACCCAAA GATCCAAGCG CTGCTTCTTA CTGTTCTGGC TACACTGGTA
    AAATATACTA CAGATGAATT TGATCAACGG ATTCTTTATG AATATCTAGC AGAGGCCAGC
    GTTGTGTTTC CCAAAGTGTT TCCTGTTGTG CACAATTTGT TGGACTCTAA GATCAATACC
    CTCTTGTCTT TATGCCAAGA TCCAAATTTG TTAAATCCAA TCCATGGAAT TGTACAGAGT
    GTGGTGTACC ATGAGGAGTC CCCGCCACAG TACCAGACAT CTTACCTGCA AAGTTTTGGT
    TTTAATGGCT TATGGCGGTT TGCAGGACCC TTTTCAAAGC AAACACAAAT CCCTGACTAT
    GCTGAGCTCA TTGTTAAATT TCTTGATGCC TTGATTGACA CGTACTTGCC TGGAATTGAT
    GAAGAAACCA GTGAGGAGTC CCTCCTGACA CCCACATCTC CTTACCCTCC TGCACTGCAG
    AGCCAGCTTA GTATCACTGC CAACCTTAAC CTCTCTAATT CCATGACCTC ACTTGCAACT
    TCCCAGCATT CCCCAGGACT CGACAAGGAG AACGTTGAAC TCTCCCCCAC CGCTGGCCAC
    TGTAACAGTG GACGGACTCG CCATGGATCC GCAAGCCAAG TGCAGAAGCA AAGAAGTGCT
    GGCAGTTTCA AACGTAATAG CATTAAGAAG ATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45703
    Clone ID Related Accession (Same CDS sequence) XM_006532437.4 , XM_006532437.3
    Accession Version XM_006532437.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532437.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGGCAGA TAGAAGCTCC TGTCATTCTC TCTACCTTTA TGGAGTAGGA
    TGTGAAATGT CTGCTACTGG AAATACCACT CAGATGTCAG TTGATCATGA TGAGTTCCTG
    CGTGCCTGTA CTCCTGGAGC ATCTCTGCGG AAAGGAAGAG GGAATTCCTC CATGGATAGC
    ACAGCAGGGT GCAGTGGAAC CCCACCTATA TGCCGACAAG CCCAGACCAA GCTAGAAGTG
    GCCTTATACA TGTTTCTGTG GAATCCTGAC ACTGAAGCTG TTCTGGTTGC TATGTCCTGT
    TTCCGCCACC TCTGTGAAGA AGCAGATATT CGATGTGGGG TAGATGAAGT GTCAGTACAC
    AACTTCTTGC CCAACTATAA CACATTCATG GAATTTGCCT CAGTCAGCAA TATGATGTCA
    ACAGGAAGAG CAGCGCTTCA GAAAAGAGTG ATGGCCCTGC TAAGGCGCAT TGAGCACCCT
    ACTGCAGGAA ACATTGAGGC CTGGGAAGAT ACACATGCAA AGTGGGAACA GGCTACAAAA
    CTAATCCTTA ACTACCCAAA AGCCAAAATG GAAGATGGCC AGGCTGCAGA AAGTCTTCAT
    AAGACCATTG TTAAGAGACG GATGTCCCAT GTCAGTGGAG GAGGCTCCAT AGACTTGTCT
    GACACAGACT CCCTGCAGGA GTGGATCAAC ATGACTGGCT TCCTTTGTGC CCTTGGTGGG
    GTGTGCCTGC AACAGAGAAG CAGCTCTGGC CTAGCGACAT ACAGCCCACC TATGGGCGCT
    GTCAGTGAAC GCAAAGGGTC TATGATTTCT GTAATGTCTT CTGAAGGGAA TATTGATTCA
    CCTGTCAGCA GATTTATGGA CCGGCTTCTG TCCTTAATGG TGTGTAACCA CGAGAAAGTG
    GGGCTTCAGA TACGGACCAA TGTTAAGGAC CTGGTGGGTC TGGAGTTGAG TCCTGCTCTG
    TATCCAATGC TGTTTAACAA ACTGAAGAAT ACCATCAGCA AGTTTTTTGA CTCTCAAGGA
    CAGGTATTAC TAAGTGACAG CAATACTCAG TTTGTAGAGC AAACCATAGC CATAATGAAA
    AACCTACTGG ATAATCATAC TGAAGGCAGC TCTGAGCATC TGGGACAAGC GAGCATTGAG
    ACAATGATGC TAAATCTGGT CAGATACGTT CGTGTTCTTG GGAATATGGT CCATGCAATA
    CAAATAAAAA CAAAATTGTG TCAGTTAGTT GAAGTCATGA TGGCAAGAAG AGATGACCTC
    TCGTTTTGTC AAGAGATGAA ATTTAGGAAT AAGATGGTAG AATATCTAAC AGACTGGGTT
    ATGGGAACAT CAAACCAAGC AGCAGATGAT GACATAAAAT GTCTTACGAG AGATCTGGAC
    CAAGCAAGCA TGGAAGCAGT AGTTTCTCTC CTCGCTGGTC TTCCACTGCA GCCCGAAGAA
    GGAGATGGGG TGGAGCTGAT GGAGGCCAAA TCACAGTTAT TCCTTAAGTA CTTCACATTA
    TTTATGAACC TTTTGAATGA CTGTAGTGAA GTTGAAGATG AAAATGCACA AACGGGTGGC
    AGGAAACGTG GCATGTCTCG GAGGCTGGCA TCCCTGAGGC ACTGTACAGT GCTTGCAATG
    TCAAACTTAC TCAATGCTAA TGTGGACAGT GGACTCATGC ACTCCATAGG CTTAGGTTAT
    CACAAGGATC TTCAGACAAG AGCTACGTTT ATGGAAGTTC TGACAAAAAT CCTTCAACAA
    GGAACAGAAT TCGATACACT TGCGGAAACG GTGTTGGCAG ATCGATTTGA GAGACTTGTG
    GAACTGGTCA CAATGATGGG AGACCAGGGA GAACTCCCTA TAGCTATGGC TCTTGCCAAT
    GTGGTCCCTT GTTCTCAGTG GGATGAGCTG GCTCGGGTTC TGGTCACTCT GTTTGATTCC
    CGGCATTTAC TCTACCAGCT GCTCTGGAAC ATGTTTTCTA AGGAGGTAGA ATTGGCAGAC
    TCCATGCAGA CTCTCTTTCG AGGCAACAGC TTGGCCAGTA AGATAATGAC ATTCTGCTTC
    AAGGTGTATG GTGCTACTTA CCTACAAAAG CTCTTGGACC CTTTATTACG AGTCATCATC
    ACATCTTCGG ATTGGCAACA TGTTAGCTTT GAAGTGGATC CTACCAGGTT AGAACCCTCT
    GAGAGCCTTG AGGAGAACCA GAGGAACCTC CTTCAGATGA CAGAGAAGTT CTTCCATGCC
    ATCATCAGCT CTTCCTCAGA ATTCCCCTCA CAGCTTCGAA GTGTCTGCCA TTGTTTGTAC
    CAGGCAACTT GCCACTCCCT ACTGAATAAA GCTACAGTAA AAGAAAGAAA GGAAAACAAA
    AAATCAGTGG TTAGCCAGCG CTTCCCTCAG AACAGCATCG GTGCCGTAGG AAGTGCCATG
    TTTCTCAGAT TCATCAATCC TGCCATTGTC TCGCCGTATG AAGCAGGGAT TTTAGATAAA
    AAGCCACCAC CTAGAATTGA AAGGGGCTTG AAGTTAATGT CAAAGGTACT TCAGAGCATT
    GCCAATCATG TACTGTTCAC AAAAGAAGAG CATATGAGAC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ACTTGGCACG AAGGTTTTTC CTAGATATAG CATCAGATTG TCCCACAAGT
    GATGCAGTAA ACCATAGTCT TTCCTTCATC AGCGATGGCA ATGTGCTTGC TTTACATCGG
    CTGCTTTGGA ACAATCAGGA GAAAATTGGC CAGTATCTTT CCAGTAACAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCCACACTTC TCGCGTATCT GGGTCCTCCG
    GAGCACAAGC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTT
    GAGGAATTTA TGACCAGGCA CCAGGTACAT GAGAAAGAAG AGTTCAAGGC CTTGAAAACG
    TTAAGCATCT TCTACCAAGC TGGCACCTCC AAAGCTGGGA ATCCTATTTT TTATTATGTT
    GCACGGAGGT TCAAAACTGG CCAGATCAAT GGTGATCTGC TGATATACCA TGTCTTGCTG
    ACTTTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACTGGG
    CCTAGCAATC GCTTTAAAAC AGACTTCCTC TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT
    GCCTATGACA ACGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAT
    ACCAAATACC ATGAGCGGCT GCTGACTGGC CTCAAGGGCA GCAAAAGGCT CATTTTCATC
    GACTGTCCTG GGAAGCTGGC TGAACATATA GAGCATGAGC AACAGAAACT CCCTGCTGCT
    ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT TTCCACAATG CTCTCAAGTT AGCTCACAAG
    GACACCAAAG TTTCTATTAA GGTTGGTTCT ACTGCTGTTC AGGTAACTTC AGCAGAAAGA
    ACAAAAGTTT TGGGGCAATC CGTCTTTTTA AATGATATTT ACTATGCTTC TGAAATCGAA
    GAGATCTGCC TAGTTGATGA GAACCAGTTC ACTCTAACCA TTGCAAACCA AGGCACACCA
    CTGACCTTCA TGCACCAAGA ATGTGAAGCC ATTGTTCAGT CTATCATTCA TATCAGGACC
    CGCTGGGAGC TGTCACAACC TGACTCCATC CCTCAGCATA CCAAGATTCG ACCAAAGGAT
    GTCCCTGGGA CACTGCTTAA TATTGCGTTA CTGAATTTGG GCAGTTCAGA CCCTAGTTTA
    CGGTCTGCTG CCTATAATCT CCTGTGTGCC TTAACCTGCA CCTTTAATTT AAAGATTGAA
    GGTCAGTTGC TAGAGACATC GGGGTTATGC ATCCCTGCCA ACAACACACT CTTCATTGTC
    TCTATCAGTA AGACGCTCGC AGCCAATGAG CCACACCTCA CCTTAGAGTT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAATTGAAGC ACCTTTGTTT GGAATATATG
    ACCCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCATA ATGATGATGC CAAACGACAA
    AGGGTTACTG CCATCCTTGA TAAGCTAATA ACAATGACTA TAAACGAGAA GCAGATGTAT
    CCTTCTATTC AAGCAAAAAT CTGGGGGAGC CTTGGGCAGA TCACAGACCT GCTGGATGTT
    GTGCTTGACA GTTTCATCAA GACCAGTGCG ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG
    GTGATGGCAG ACACAGCTGT GGCTTTAGCT TCTGGAAATG TGAAATTGGT GTCGAGTAAG
    GTTATTGGAA GGATGTGTAA AATAATTGAC AAGACTTGCT TATCCCCAAC TCCAACTTTA
    GAACAACATC TTATGTGGGA CGACATTGCC ATTTTAGCCC GCTACATGCT GATGCTGTCC
    TTCAACAACT CCCTCGATGT GGCGGCTCAT CTGCCCTATC TCTTCCATGT TGTCACTTTC
    TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA GCTTCCACAC ATGGGCTGCT CATCAATATC
    ATTCACTCTC TGTGTACTTG TTCTCAGCTT CACTTTAGTG AAGAGACCAA GCAAGTTTTG
    AGGCTCAGTC TAACAGAGTT CTCGTTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT TCCAGTTACC GGGACCGCTC CTTCTCCCCT
    GGCTCCTATG AGAGGGAGAC TTTTGCTTTG ACGTCCCTGG AAACAGTCAC AGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGATT TGCGTTTCAG TATAACCCAT CGCTGCAGCC AAGAGCTCTT
    GTGGTGTTTG GCTGTATTAG CAAACGAGTG TCTCATGGGC AGATAAAGCA GATTATCCGA
    ATTCTTAGCA AGGCACTTGA AAGTTGTTTA AAAGGACCTG ACACTTACAA CAGTCAAGTT
    CTGATAGAAT CTACGGTGAT AGCACTAACA AAATTACAGC CGCTTCTTAA TAAGGACTCG
    CCCCTGCACA AAGCCCTCTT TTGGGTTGCT GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC
    TTGTATTCAG CCGGCACTGC ACTTCTGGAA CAAAACCTGC ACACCTTGGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC GGAAGAAGTA TTTATGGCGA TCCGGAATCC TCTGGAATGG
    CACTGCAAGC AGATGGATCA CTTTGTCGGA CTCAACTTCA ACTCTAACTT TAACTTTGCA
    CTAGTTGGAC ACCTCTTAAA AGGGTACAGG CATCCTTCAC CTGCCATTGT TGCAAGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTC GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CACAGAGTGT GGCTTACTTA GCAGCTCTAC TCACAGTGTC TGAAGAGGTT
    CGAAGTCGCT GTAGCCTCAA GCATAGGAAG TCCCTTCTTC TTACTGATAT TTCAATGGAA
    AATGTTCCTA TGGATACATA TCCCATTCAT CACGGTGACC CCAGCTATAG GACACTAAAG
    GAGACCCAGC CATGGTCCTC TCCCAAAGGT TCAGAAGGAT ACCTTGCAGC CACCTACCCA
    GCTGTAGGCC AAACCAGTCC CCGAGCCAGG AAATCCATGA GCTTGGATAT GGGACAGCCT
    TCTCAGGCCA ACACAAAAAA ATTGCTTGGA ACAAGAAAAA GTTTTGATCA CTTGATATCT
    GACACAAAGG CTCCTAAAAG ACAAGAAATG GAATCAGGGA TTACAACACC CCCGAAAATG
    AGAAGAGTGG CAGAGACTGA CTATGAAATG GCAGAAACCC AAAGGATTCC CTCCTCGCAG
    CAGCACCCAC ATTTACGGAA AGTCTCAGTC TCTGAATCAA ATGTTCTTTT GGATGAAGAA
    GTTCTTACCG ACCCAAAGAT CCAAGCGCTG CTTCTTACTG TTCTGGCTAC ACTGGTAAAA
    TATACTACAG ATGAATTTGA TCAACGGATT CTTTATGAAT ATCTAGCAGA GGCCAGCGTT
    GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC AATTTGTTGG ACTCTAAGAT CAATACCCTC
    TTGTCTTTAT GCCAAGATCC AAATTTGTTA AATCCAATCC ATGGAATTGT ACAGAGTGTG
    GTGTACCATG AGGAGTCCCC GCCACAGTAC CAGACATCTT ACCTGCAAAG TTTTGGTTTT
    AATGGCTTAT GGCGGTTTGC AGGACCCTTT TCAAAGCAAA CACAAATCCC TGACTATGCT
    GAGCTCATTG TTAAATTTCT TGATGCCTTG ATTGACACGT ACTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ACCCTCCTGC ACTGCAGAGC
    CAGCTTAGTA TCACTGCCAA CCTTAACCTC TCTAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGACTCGA CAAGGAGAAC GTTGAACTCT CCCCCACCGC TGGCCACTGT
    AACAGTGGAC GGACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532500.1
    CDS407..8962
    Translation

    Target ORF information:

    RefSeq Version XM_006532437.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromin 1 (Nf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532437.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGGCAGA TAGAAGCTCC TGTCATTCTC TCTACCTTTA TGGAGTAGGA
    TGTGAAATGT CTGCTACTGG AAATACCACT CAGATGTCAG TTGATCATGA TGAGTTCCTG
    CGTGCCTGTA CTCCTGGAGC ATCTCTGCGG AAAGGAAGAG GGAATTCCTC CATGGATAGC
    ACAGCAGGGT GCAGTGGAAC CCCACCTATA TGCCGACAAG CCCAGACCAA GCTAGAAGTG
    GCCTTATACA TGTTTCTGTG GAATCCTGAC ACTGAAGCTG TTCTGGTTGC TATGTCCTGT
    TTCCGCCACC TCTGTGAAGA AGCAGATATT CGATGTGGGG TAGATGAAGT GTCAGTACAC
    AACTTCTTGC CCAACTATAA CACATTCATG GAATTTGCCT CAGTCAGCAA TATGATGTCA
    ACAGGAAGAG CAGCGCTTCA GAAAAGAGTG ATGGCCCTGC TAAGGCGCAT TGAGCACCCT
    ACTGCAGGAA ACATTGAGGC CTGGGAAGAT ACACATGCAA AGTGGGAACA GGCTACAAAA
    CTAATCCTTA ACTACCCAAA AGCCAAAATG GAAGATGGCC AGGCTGCAGA AAGTCTTCAT
    AAGACCATTG TTAAGAGACG GATGTCCCAT GTCAGTGGAG GAGGCTCCAT AGACTTGTCT
    GACACAGACT CCCTGCAGGA GTGGATCAAC ATGACTGGCT TCCTTTGTGC CCTTGGTGGG
    GTGTGCCTGC AACAGAGAAG CAGCTCTGGC CTAGCGACAT ACAGCCCACC TATGGGCGCT
    GTCAGTGAAC GCAAAGGGTC TATGATTTCT GTAATGTCTT CTGAAGGGAA TATTGATTCA
    CCTGTCAGCA GATTTATGGA CCGGCTTCTG TCCTTAATGG TGTGTAACCA CGAGAAAGTG
    GGGCTTCAGA TACGGACCAA TGTTAAGGAC CTGGTGGGTC TGGAGTTGAG TCCTGCTCTG
    TATCCAATGC TGTTTAACAA ACTGAAGAAT ACCATCAGCA AGTTTTTTGA CTCTCAAGGA
    CAGGTATTAC TAAGTGACAG CAATACTCAG TTTGTAGAGC AAACCATAGC CATAATGAAA
    AACCTACTGG ATAATCATAC TGAAGGCAGC TCTGAGCATC TGGGACAAGC GAGCATTGAG
    ACAATGATGC TAAATCTGGT CAGATACGTT CGTGTTCTTG GGAATATGGT CCATGCAATA
    CAAATAAAAA CAAAATTGTG TCAGTTAGTT GAAGTCATGA TGGCAAGAAG AGATGACCTC
    TCGTTTTGTC AAGAGATGAA ATTTAGGAAT AAGATGGTAG AATATCTAAC AGACTGGGTT
    ATGGGAACAT CAAACCAAGC AGCAGATGAT GACATAAAAT GTCTTACGAG AGATCTGGAC
    CAAGCAAGCA TGGAAGCAGT AGTTTCTCTC CTCGCTGGTC TTCCACTGCA GCCCGAAGAA
    GGAGATGGGG TGGAGCTGAT GGAGGCCAAA TCACAGTTAT TCCTTAAGTA CTTCACATTA
    TTTATGAACC TTTTGAATGA CTGTAGTGAA GTTGAAGATG AAAATGCACA AACGGGTGGC
    AGGAAACGTG GCATGTCTCG GAGGCTGGCA TCCCTGAGGC ACTGTACAGT GCTTGCAATG
    TCAAACTTAC TCAATGCTAA TGTGGACAGT GGACTCATGC ACTCCATAGG CTTAGGTTAT
    CACAAGGATC TTCAGACAAG AGCTACGTTT ATGGAAGTTC TGACAAAAAT CCTTCAACAA
    GGAACAGAAT TCGATACACT TGCGGAAACG GTGTTGGCAG ATCGATTTGA GAGACTTGTG
    GAACTGGTCA CAATGATGGG AGACCAGGGA GAACTCCCTA TAGCTATGGC TCTTGCCAAT
    GTGGTCCCTT GTTCTCAGTG GGATGAGCTG GCTCGGGTTC TGGTCACTCT GTTTGATTCC
    CGGCATTTAC TCTACCAGCT GCTCTGGAAC ATGTTTTCTA AGGAGGTAGA ATTGGCAGAC
    TCCATGCAGA CTCTCTTTCG AGGCAACAGC TTGGCCAGTA AGATAATGAC ATTCTGCTTC
    AAGGTGTATG GTGCTACTTA CCTACAAAAG CTCTTGGACC CTTTATTACG AGTCATCATC
    ACATCTTCGG ATTGGCAACA TGTTAGCTTT GAAGTGGATC CTACCAGGTT AGAACCCTCT
    GAGAGCCTTG AGGAGAACCA GAGGAACCTC CTTCAGATGA CAGAGAAGTT CTTCCATGCC
    ATCATCAGCT CTTCCTCAGA ATTCCCCTCA CAGCTTCGAA GTGTCTGCCA TTGTTTGTAC
    CAGGCAACTT GCCACTCCCT ACTGAATAAA GCTACAGTAA AAGAAAGAAA GGAAAACAAA
    AAATCAGTGG TTAGCCAGCG CTTCCCTCAG AACAGCATCG GTGCCGTAGG AAGTGCCATG
    TTTCTCAGAT TCATCAATCC TGCCATTGTC TCGCCGTATG AAGCAGGGAT TTTAGATAAA
    AAGCCACCAC CTAGAATTGA AAGGGGCTTG AAGTTAATGT CAAAGGTACT TCAGAGCATT
    GCCAATCATG TACTGTTCAC AAAAGAAGAG CATATGAGAC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ACTTGGCACG AAGGTTTTTC CTAGATATAG CATCAGATTG TCCCACAAGT
    GATGCAGTAA ACCATAGTCT TTCCTTCATC AGCGATGGCA ATGTGCTTGC TTTACATCGG
    CTGCTTTGGA ACAATCAGGA GAAAATTGGC CAGTATCTTT CCAGTAACAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCCACACTTC TCGCGTATCT GGGTCCTCCG
    GAGCACAAGC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTT
    GAGGAATTTA TGACCAGGCA CCAGGTACAT GAGAAAGAAG AGTTCAAGGC CTTGAAAACG
    TTAAGCATCT TCTACCAAGC TGGCACCTCC AAAGCTGGGA ATCCTATTTT TTATTATGTT
    GCACGGAGGT TCAAAACTGG CCAGATCAAT GGTGATCTGC TGATATACCA TGTCTTGCTG
    ACTTTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACTGGG
    CCTAGCAATC GCTTTAAAAC AGACTTCCTC TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT
    GCCTATGACA ACGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAT
    ACCAAATACC ATGAGCGGCT GCTGACTGGC CTCAAGGGCA GCAAAAGGCT CATTTTCATC
    GACTGTCCTG GGAAGCTGGC TGAACATATA GAGCATGAGC AACAGAAACT CCCTGCTGCT
    ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT TTCCACAATG CTCTCAAGTT AGCTCACAAG
    GACACCAAAG TTTCTATTAA GGTTGGTTCT ACTGCTGTTC AGGTAACTTC AGCAGAAAGA
    ACAAAAGTTT TGGGGCAATC CGTCTTTTTA AATGATATTT ACTATGCTTC TGAAATCGAA
    GAGATCTGCC TAGTTGATGA GAACCAGTTC ACTCTAACCA TTGCAAACCA AGGCACACCA
    CTGACCTTCA TGCACCAAGA ATGTGAAGCC ATTGTTCAGT CTATCATTCA TATCAGGACC
    CGCTGGGAGC TGTCACAACC TGACTCCATC CCTCAGCATA CCAAGATTCG ACCAAAGGAT
    GTCCCTGGGA CACTGCTTAA TATTGCGTTA CTGAATTTGG GCAGTTCAGA CCCTAGTTTA
    CGGTCTGCTG CCTATAATCT CCTGTGTGCC TTAACCTGCA CCTTTAATTT AAAGATTGAA
    GGTCAGTTGC TAGAGACATC GGGGTTATGC ATCCCTGCCA ACAACACACT CTTCATTGTC
    TCTATCAGTA AGACGCTCGC AGCCAATGAG CCACACCTCA CCTTAGAGTT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAATTGAAGC ACCTTTGTTT GGAATATATG
    ACCCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCATA ATGATGATGC CAAACGACAA
    AGGGTTACTG CCATCCTTGA TAAGCTAATA ACAATGACTA TAAACGAGAA GCAGATGTAT
    CCTTCTATTC AAGCAAAAAT CTGGGGGAGC CTTGGGCAGA TCACAGACCT GCTGGATGTT
    GTGCTTGACA GTTTCATCAA GACCAGTGCG ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG
    GTGATGGCAG ACACAGCTGT GGCTTTAGCT TCTGGAAATG TGAAATTGGT GTCGAGTAAG
    GTTATTGGAA GGATGTGTAA AATAATTGAC AAGACTTGCT TATCCCCAAC TCCAACTTTA
    GAACAACATC TTATGTGGGA CGACATTGCC ATTTTAGCCC GCTACATGCT GATGCTGTCC
    TTCAACAACT CCCTCGATGT GGCGGCTCAT CTGCCCTATC TCTTCCATGT TGTCACTTTC
    TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA GCTTCCACAC ATGGGCTGCT CATCAATATC
    ATTCACTCTC TGTGTACTTG TTCTCAGCTT CACTTTAGTG AAGAGACCAA GCAAGTTTTG
    AGGCTCAGTC TAACAGAGTT CTCGTTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT TCCAGTTACC GGGACCGCTC CTTCTCCCCT
    GGCTCCTATG AGAGGGAGAC TTTTGCTTTG ACGTCCCTGG AAACAGTCAC AGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGATT TGCGTTTCAG TATAACCCAT CGCTGCAGCC AAGAGCTCTT
    GTGGTGTTTG GCTGTATTAG CAAACGAGTG TCTCATGGGC AGATAAAGCA GATTATCCGA
    ATTCTTAGCA AGGCACTTGA AAGTTGTTTA AAAGGACCTG ACACTTACAA CAGTCAAGTT
    CTGATAGAAT CTACGGTGAT AGCACTAACA AAATTACAGC CGCTTCTTAA TAAGGACTCG
    CCCCTGCACA AAGCCCTCTT TTGGGTTGCT GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC
    TTGTATTCAG CCGGCACTGC ACTTCTGGAA CAAAACCTGC ACACCTTGGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC GGAAGAAGTA TTTATGGCGA TCCGGAATCC TCTGGAATGG
    CACTGCAAGC AGATGGATCA CTTTGTCGGA CTCAACTTCA ACTCTAACTT TAACTTTGCA
    CTAGTTGGAC ACCTCTTAAA AGGGTACAGG CATCCTTCAC CTGCCATTGT TGCAAGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTC GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CACAGAGTGT GGCTTACTTA GCAGCTCTAC TCACAGTGTC TGAAGAGGTT
    CGAAGTCGCT GTAGCCTCAA GCATAGGAAG TCCCTTCTTC TTACTGATAT TTCAATGGAA
    AATGTTCCTA TGGATACATA TCCCATTCAT CACGGTGACC CCAGCTATAG GACACTAAAG
    GAGACCCAGC CATGGTCCTC TCCCAAAGGT TCAGAAGGAT ACCTTGCAGC CACCTACCCA
    GCTGTAGGCC AAACCAGTCC CCGAGCCAGG AAATCCATGA GCTTGGATAT GGGACAGCCT
    TCTCAGGCCA ACACAAAAAA ATTGCTTGGA ACAAGAAAAA GTTTTGATCA CTTGATATCT
    GACACAAAGG CTCCTAAAAG ACAAGAAATG GAATCAGGGA TTACAACACC CCCGAAAATG
    AGAAGAGTGG CAGAGACTGA CTATGAAATG GCAGAAACCC AAAGGATTCC CTCCTCGCAG
    CAGCACCCAC ATTTACGGAA AGTCTCAGTC TCTGAATCAA ATGTTCTTTT GGATGAAGAA
    GTTCTTACCG ACCCAAAGAT CCAAGCGCTG CTTCTTACTG TTCTGGCTAC ACTGGTAAAA
    TATACTACAG ATGAATTTGA TCAACGGATT CTTTATGAAT ATCTAGCAGA GGCCAGCGTT
    GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC AATTTGTTGG ACTCTAAGAT CAATACCCTC
    TTGTCTTTAT GCCAAGATCC AAATTTGTTA AATCCAATCC ATGGAATTGT ACAGAGTGTG
    GTGTACCATG AGGAGTCCCC GCCACAGTAC CAGACATCTT ACCTGCAAAG TTTTGGTTTT
    AATGGCTTAT GGCGGTTTGC AGGACCCTTT TCAAAGCAAA CACAAATCCC TGACTATGCT
    GAGCTCATTG TTAAATTTCT TGATGCCTTG ATTGACACGT ACTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ACCCTCCTGC ACTGCAGAGC
    CAGCTTAGTA TCACTGCCAA CCTTAACCTC TCTAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGACTCGA CAAGGAGAAC GTTGAACTCT CCCCCACCGC TGGCCACTGT
    AACAGTGGAC GGACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45703
    Clone ID Related Accession (Same CDS sequence) XM_006532437.4 , XM_006532437.3
    Accession Version XM_006532437.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532437.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGGCAGA TAGAAGCTCC TGTCATTCTC TCTACCTTTA TGGAGTAGGA
    TGTGAAATGT CTGCTACTGG AAATACCACT CAGATGTCAG TTGATCATGA TGAGTTCCTG
    CGTGCCTGTA CTCCTGGAGC ATCTCTGCGG AAAGGAAGAG GGAATTCCTC CATGGATAGC
    ACAGCAGGGT GCAGTGGAAC CCCACCTATA TGCCGACAAG CCCAGACCAA GCTAGAAGTG
    GCCTTATACA TGTTTCTGTG GAATCCTGAC ACTGAAGCTG TTCTGGTTGC TATGTCCTGT
    TTCCGCCACC TCTGTGAAGA AGCAGATATT CGATGTGGGG TAGATGAAGT GTCAGTACAC
    AACTTCTTGC CCAACTATAA CACATTCATG GAATTTGCCT CAGTCAGCAA TATGATGTCA
    ACAGGAAGAG CAGCGCTTCA GAAAAGAGTG ATGGCCCTGC TAAGGCGCAT TGAGCACCCT
    ACTGCAGGAA ACATTGAGGC CTGGGAAGAT ACACATGCAA AGTGGGAACA GGCTACAAAA
    CTAATCCTTA ACTACCCAAA AGCCAAAATG GAAGATGGCC AGGCTGCAGA AAGTCTTCAT
    AAGACCATTG TTAAGAGACG GATGTCCCAT GTCAGTGGAG GAGGCTCCAT AGACTTGTCT
    GACACAGACT CCCTGCAGGA GTGGATCAAC ATGACTGGCT TCCTTTGTGC CCTTGGTGGG
    GTGTGCCTGC AACAGAGAAG CAGCTCTGGC CTAGCGACAT ACAGCCCACC TATGGGCGCT
    GTCAGTGAAC GCAAAGGGTC TATGATTTCT GTAATGTCTT CTGAAGGGAA TATTGATTCA
    CCTGTCAGCA GATTTATGGA CCGGCTTCTG TCCTTAATGG TGTGTAACCA CGAGAAAGTG
    GGGCTTCAGA TACGGACCAA TGTTAAGGAC CTGGTGGGTC TGGAGTTGAG TCCTGCTCTG
    TATCCAATGC TGTTTAACAA ACTGAAGAAT ACCATCAGCA AGTTTTTTGA CTCTCAAGGA
    CAGGTATTAC TAAGTGACAG CAATACTCAG TTTGTAGAGC AAACCATAGC CATAATGAAA
    AACCTACTGG ATAATCATAC TGAAGGCAGC TCTGAGCATC TGGGACAAGC GAGCATTGAG
    ACAATGATGC TAAATCTGGT CAGATACGTT CGTGTTCTTG GGAATATGGT CCATGCAATA
    CAAATAAAAA CAAAATTGTG TCAGTTAGTT GAAGTCATGA TGGCAAGAAG AGATGACCTC
    TCGTTTTGTC AAGAGATGAA ATTTAGGAAT AAGATGGTAG AATATCTAAC AGACTGGGTT
    ATGGGAACAT CAAACCAAGC AGCAGATGAT GACATAAAAT GTCTTACGAG AGATCTGGAC
    CAAGCAAGCA TGGAAGCAGT AGTTTCTCTC CTCGCTGGTC TTCCACTGCA GCCCGAAGAA
    GGAGATGGGG TGGAGCTGAT GGAGGCCAAA TCACAGTTAT TCCTTAAGTA CTTCACATTA
    TTTATGAACC TTTTGAATGA CTGTAGTGAA GTTGAAGATG AAAATGCACA AACGGGTGGC
    AGGAAACGTG GCATGTCTCG GAGGCTGGCA TCCCTGAGGC ACTGTACAGT GCTTGCAATG
    TCAAACTTAC TCAATGCTAA TGTGGACAGT GGACTCATGC ACTCCATAGG CTTAGGTTAT
    CACAAGGATC TTCAGACAAG AGCTACGTTT ATGGAAGTTC TGACAAAAAT CCTTCAACAA
    GGAACAGAAT TCGATACACT TGCGGAAACG GTGTTGGCAG ATCGATTTGA GAGACTTGTG
    GAACTGGTCA CAATGATGGG AGACCAGGGA GAACTCCCTA TAGCTATGGC TCTTGCCAAT
    GTGGTCCCTT GTTCTCAGTG GGATGAGCTG GCTCGGGTTC TGGTCACTCT GTTTGATTCC
    CGGCATTTAC TCTACCAGCT GCTCTGGAAC ATGTTTTCTA AGGAGGTAGA ATTGGCAGAC
    TCCATGCAGA CTCTCTTTCG AGGCAACAGC TTGGCCAGTA AGATAATGAC ATTCTGCTTC
    AAGGTGTATG GTGCTACTTA CCTACAAAAG CTCTTGGACC CTTTATTACG AGTCATCATC
    ACATCTTCGG ATTGGCAACA TGTTAGCTTT GAAGTGGATC CTACCAGGTT AGAACCCTCT
    GAGAGCCTTG AGGAGAACCA GAGGAACCTC CTTCAGATGA CAGAGAAGTT CTTCCATGCC
    ATCATCAGCT CTTCCTCAGA ATTCCCCTCA CAGCTTCGAA GTGTCTGCCA TTGTTTGTAC
    CAGGCAACTT GCCACTCCCT ACTGAATAAA GCTACAGTAA AAGAAAGAAA GGAAAACAAA
    AAATCAGTGG TTAGCCAGCG CTTCCCTCAG AACAGCATCG GTGCCGTAGG AAGTGCCATG
    TTTCTCAGAT TCATCAATCC TGCCATTGTC TCGCCGTATG AAGCAGGGAT TTTAGATAAA
    AAGCCACCAC CTAGAATTGA AAGGGGCTTG AAGTTAATGT CAAAGGTACT TCAGAGCATT
    GCCAATCATG TACTGTTCAC AAAAGAAGAG CATATGAGAC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ACTTGGCACG AAGGTTTTTC CTAGATATAG CATCAGATTG TCCCACAAGT
    GATGCAGTAA ACCATAGTCT TTCCTTCATC AGCGATGGCA ATGTGCTTGC TTTACATCGG
    CTGCTTTGGA ACAATCAGGA GAAAATTGGC CAGTATCTTT CCAGTAACAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCCACACTTC TCGCGTATCT GGGTCCTCCG
    GAGCACAAGC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTT
    GAGGAATTTA TGACCAGGCA CCAGGTACAT GAGAAAGAAG AGTTCAAGGC CTTGAAAACG
    TTAAGCATCT TCTACCAAGC TGGCACCTCC AAAGCTGGGA ATCCTATTTT TTATTATGTT
    GCACGGAGGT TCAAAACTGG CCAGATCAAT GGTGATCTGC TGATATACCA TGTCTTGCTG
    ACTTTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACTGGG
    CCTAGCAATC GCTTTAAAAC AGACTTCCTC TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT
    GCCTATGACA ACGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAT
    ACCAAATACC ATGAGCGGCT GCTGACTGGC CTCAAGGGCA GCAAAAGGCT CATTTTCATC
    GACTGTCCTG GGAAGCTGGC TGAACATATA GAGCATGAGC AACAGAAACT CCCTGCTGCT
    ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT TTCCACAATG CTCTCAAGTT AGCTCACAAG
    GACACCAAAG TTTCTATTAA GGTTGGTTCT ACTGCTGTTC AGGTAACTTC AGCAGAAAGA
    ACAAAAGTTT TGGGGCAATC CGTCTTTTTA AATGATATTT ACTATGCTTC TGAAATCGAA
    GAGATCTGCC TAGTTGATGA GAACCAGTTC ACTCTAACCA TTGCAAACCA AGGCACACCA
    CTGACCTTCA TGCACCAAGA ATGTGAAGCC ATTGTTCAGT CTATCATTCA TATCAGGACC
    CGCTGGGAGC TGTCACAACC TGACTCCATC CCTCAGCATA CCAAGATTCG ACCAAAGGAT
    GTCCCTGGGA CACTGCTTAA TATTGCGTTA CTGAATTTGG GCAGTTCAGA CCCTAGTTTA
    CGGTCTGCTG CCTATAATCT CCTGTGTGCC TTAACCTGCA CCTTTAATTT AAAGATTGAA
    GGTCAGTTGC TAGAGACATC GGGGTTATGC ATCCCTGCCA ACAACACACT CTTCATTGTC
    TCTATCAGTA AGACGCTCGC AGCCAATGAG CCACACCTCA CCTTAGAGTT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAATTGAAGC ACCTTTGTTT GGAATATATG
    ACCCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCATA ATGATGATGC CAAACGACAA
    AGGGTTACTG CCATCCTTGA TAAGCTAATA ACAATGACTA TAAACGAGAA GCAGATGTAT
    CCTTCTATTC AAGCAAAAAT CTGGGGGAGC CTTGGGCAGA TCACAGACCT GCTGGATGTT
    GTGCTTGACA GTTTCATCAA GACCAGTGCG ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG
    GTGATGGCAG ACACAGCTGT GGCTTTAGCT TCTGGAAATG TGAAATTGGT GTCGAGTAAG
    GTTATTGGAA GGATGTGTAA AATAATTGAC AAGACTTGCT TATCCCCAAC TCCAACTTTA
    GAACAACATC TTATGTGGGA CGACATTGCC ATTTTAGCCC GCTACATGCT GATGCTGTCC
    TTCAACAACT CCCTCGATGT GGCGGCTCAT CTGCCCTATC TCTTCCATGT TGTCACTTTC
    TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA GCTTCCACAC ATGGGCTGCT CATCAATATC
    ATTCACTCTC TGTGTACTTG TTCTCAGCTT CACTTTAGTG AAGAGACCAA GCAAGTTTTG
    AGGCTCAGTC TAACAGAGTT CTCGTTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT TCCAGTTACC GGGACCGCTC CTTCTCCCCT
    GGCTCCTATG AGAGGGAGAC TTTTGCTTTG ACGTCCCTGG AAACAGTCAC AGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGATT TGCGTTTCAG TATAACCCAT CGCTGCAGCC AAGAGCTCTT
    GTGGTGTTTG GCTGTATTAG CAAACGAGTG TCTCATGGGC AGATAAAGCA GATTATCCGA
    ATTCTTAGCA AGGCACTTGA AAGTTGTTTA AAAGGACCTG ACACTTACAA CAGTCAAGTT
    CTGATAGAAT CTACGGTGAT AGCACTAACA AAATTACAGC CGCTTCTTAA TAAGGACTCG
    CCCCTGCACA AAGCCCTCTT TTGGGTTGCT GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC
    TTGTATTCAG CCGGCACTGC ACTTCTGGAA CAAAACCTGC ACACCTTGGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC GGAAGAAGTA TTTATGGCGA TCCGGAATCC TCTGGAATGG
    CACTGCAAGC AGATGGATCA CTTTGTCGGA CTCAACTTCA ACTCTAACTT TAACTTTGCA
    CTAGTTGGAC ACCTCTTAAA AGGGTACAGG CATCCTTCAC CTGCCATTGT TGCAAGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTC GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CACAGAGTGT GGCTTACTTA GCAGCTCTAC TCACAGTGTC TGAAGAGGTT
    CGAAGTCGCT GTAGCCTCAA GCATAGGAAG TCCCTTCTTC TTACTGATAT TTCAATGGAA
    AATGTTCCTA TGGATACATA TCCCATTCAT CACGGTGACC CCAGCTATAG GACACTAAAG
    GAGACCCAGC CATGGTCCTC TCCCAAAGGT TCAGAAGGAT ACCTTGCAGC CACCTACCCA
    GCTGTAGGCC AAACCAGTCC CCGAGCCAGG AAATCCATGA GCTTGGATAT GGGACAGCCT
    TCTCAGGCCA ACACAAAAAA ATTGCTTGGA ACAAGAAAAA GTTTTGATCA CTTGATATCT
    GACACAAAGG CTCCTAAAAG ACAAGAAATG GAATCAGGGA TTACAACACC CCCGAAAATG
    AGAAGAGTGG CAGAGACTGA CTATGAAATG GCAGAAACCC AAAGGATTCC CTCCTCGCAG
    CAGCACCCAC ATTTACGGAA AGTCTCAGTC TCTGAATCAA ATGTTCTTTT GGATGAAGAA
    GTTCTTACCG ACCCAAAGAT CCAAGCGCTG CTTCTTACTG TTCTGGCTAC ACTGGTAAAA
    TATACTACAG ATGAATTTGA TCAACGGATT CTTTATGAAT ATCTAGCAGA GGCCAGCGTT
    GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC AATTTGTTGG ACTCTAAGAT CAATACCCTC
    TTGTCTTTAT GCCAAGATCC AAATTTGTTA AATCCAATCC ATGGAATTGT ACAGAGTGTG
    GTGTACCATG AGGAGTCCCC GCCACAGTAC CAGACATCTT ACCTGCAAAG TTTTGGTTTT
    AATGGCTTAT GGCGGTTTGC AGGACCCTTT TCAAAGCAAA CACAAATCCC TGACTATGCT
    GAGCTCATTG TTAAATTTCT TGATGCCTTG ATTGACACGT ACTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ACCCTCCTGC ACTGCAGAGC
    CAGCTTAGTA TCACTGCCAA CCTTAACCTC TCTAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGACTCGA CAAGGAGAAC GTTGAACTCT CCCCCACCGC TGGCCACTGT
    AACAGTGGAC GGACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532500.1
    CDS1612..10167
    Translation

    Target ORF information:

    RefSeq Version XM_006532437.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromatosis 1 (Nf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532437.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGGCAGA TAGAAGCTCC TGTCATTCTC TCTACCTTTA TGGAGTAGGA
    TGTGAAATGT CTGCTACTGG AAATACCACT CAGATGTCAG TTGATCATGA TGAGTTCCTG
    CGTGCCTGTA CTCCTGGAGC ATCTCTGCGG AAAGGAAGAG GGAATTCCTC CATGGATAGC
    ACAGCAGGGT GCAGTGGAAC CCCACCTATA TGCCGACAAG CCCAGACCAA GCTAGAAGTG
    GCCTTATACA TGTTTCTGTG GAATCCTGAC ACTGAAGCTG TTCTGGTTGC TATGTCCTGT
    TTCCGCCACC TCTGTGAAGA AGCAGATATT CGATGTGGGG TAGATGAAGT GTCAGTACAC
    AACTTCTTGC CCAACTATAA CACATTCATG GAATTTGCCT CAGTCAGCAA TATGATGTCA
    ACAGGAAGAG CAGCGCTTCA GAAAAGAGTG ATGGCCCTGC TAAGGCGCAT TGAGCACCCT
    ACTGCAGGAA ACATTGAGGC CTGGGAAGAT ACACATGCAA AGTGGGAACA GGCTACAAAA
    CTAATCCTTA ACTACCCAAA AGCCAAAATG GAAGATGGCC AGGCTGCAGA AAGTCTTCAT
    AAGACCATTG TTAAGAGACG GATGTCCCAT GTCAGTGGAG GAGGCTCCAT AGACTTGTCT
    GACACAGACT CCCTGCAGGA GTGGATCAAC ATGACTGGCT TCCTTTGTGC CCTTGGTGGG
    GTGTGCCTGC AACAGAGAAG CAGCTCTGGC CTAGCGACAT ACAGCCCACC TATGGGCGCT
    GTCAGTGAAC GCAAAGGGTC TATGATTTCT GTAATGTCTT CTGAAGGGAA TATTGATTCA
    CCTGTCAGCA GATTTATGGA CCGGCTTCTG TCCTTAATGG TGTGTAACCA CGAGAAAGTG
    GGGCTTCAGA TACGGACCAA TGTTAAGGAC CTGGTGGGTC TGGAGTTGAG TCCTGCTCTG
    TATCCAATGC TGTTTAACAA ACTGAAGAAT ACCATCAGCA AGTTTTTTGA CTCTCAAGGA
    CAGGTATTAC TAAGTGACAG CAATACTCAG TTTGTAGAGC AAACCATAGC CATAATGAAA
    AACCTACTGG ATAATCATAC TGAAGGCAGC TCTGAGCATC TGGGACAAGC GAGCATTGAG
    ACAATGATGC TAAATCTGGT CAGATACGTT CGTGTTCTTG GGAATATGGT CCATGCAATA
    CAAATAAAAA CAAAATTGTG TCAGTTAGTT GAAGTCATGA TGGCAAGAAG AGATGACCTC
    TCGTTTTGTC AAGAGATGAA ATTTAGGAAT AAGATGGTAG AATATCTAAC AGACTGGGTT
    ATGGGAACAT CAAACCAAGC AGCAGATGAT GACATAAAAT GTCTTACGAG AGATCTGGAC
    CAAGCAAGCA TGGAAGCAGT AGTTTCTCTC CTCGCTGGTC TTCCACTGCA GCCCGAAGAA
    GGAGATGGGG TGGAGCTGAT GGAGGCCAAA TCACAGTTAT TCCTTAAGTA CTTCACATTA
    TTTATGAACC TTTTGAATGA CTGTAGTGAA GTTGAAGATG AAAATGCACA AACGGGTGGC
    AGGAAACGTG GCATGTCTCG GAGGCTGGCA TCCCTGAGGC ACTGTACAGT GCTTGCAATG
    TCAAACTTAC TCAATGCTAA TGTGGACAGT GGACTCATGC ACTCCATAGG CTTAGGTTAT
    CACAAGGATC TTCAGACAAG AGCTACGTTT ATGGAAGTTC TGACAAAAAT CCTTCAACAA
    GGAACAGAAT TCGATACACT TGCGGAAACG GTGTTGGCAG ATCGATTTGA GAGACTTGTG
    GAACTGGTCA CAATGATGGG AGACCAGGGA GAACTCCCTA TAGCTATGGC TCTTGCCAAT
    GTGGTCCCTT GTTCTCAGTG GGATGAGCTG GCTCGGGTTC TGGTCACTCT GTTTGATTCC
    CGGCATTTAC TCTACCAGCT GCTCTGGAAC ATGTTTTCTA AGGAGGTAGA ATTGGCAGAC
    TCCATGCAGA CTCTCTTTCG AGGCAACAGC TTGGCCAGTA AGATAATGAC ATTCTGCTTC
    AAGGTGTATG GTGCTACTTA CCTACAAAAG CTCTTGGACC CTTTATTACG AGTCATCATC
    ACATCTTCGG ATTGGCAACA TGTTAGCTTT GAAGTGGATC CTACCAGGTT AGAACCCTCT
    GAGAGCCTTG AGGAGAACCA GAGGAACCTC CTTCAGATGA CAGAGAAGTT CTTCCATGCC
    ATCATCAGCT CTTCCTCAGA ATTCCCCTCA CAGCTTCGAA GTGTCTGCCA TTGTTTGTAC
    CAGGCAACTT GCCACTCCCT ACTGAATAAA GCTACAGTAA AAGAAAGAAA GGAAAACAAA
    AAATCAGTGG TTAGCCAGCG CTTCCCTCAG AACAGCATCG GTGCCGTAGG AAGTGCCATG
    TTTCTCAGAT TCATCAATCC TGCCATTGTC TCGCCGTATG AAGCAGGGAT TTTAGATAAA
    AAGCCACCAC CTAGAATTGA AAGGGGCTTG AAGTTAATGT CAAAGGTACT TCAGAGCATT
    GCCAATCATG TACTGTTCAC AAAAGAAGAG CATATGAGAC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ACTTGGCACG AAGGTTTTTC CTAGATATAG CATCAGATTG TCCCACAAGT
    GATGCAGTAA ACCATAGTCT TTCCTTCATC AGCGATGGCA ATGTGCTTGC TTTACATCGG
    CTGCTTTGGA ACAATCAGGA GAAAATTGGC CAGTATCTTT CCAGTAACAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCCACACTTC TCGCGTATCT GGGTCCTCCG
    GAGCACAAGC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTT
    GAGGAATTTA TGACCAGGCA CCAGGTACAT GAGAAAGAAG AGTTCAAGGC CTTGAAAACG
    TTAAGCATCT TCTACCAAGC TGGCACCTCC AAAGCTGGGA ATCCTATTTT TTATTATGTT
    GCACGGAGGT TCAAAACTGG CCAGATCAAT GGTGATCTGC TGATATACCA TGTCTTGCTG
    ACTTTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACTGGG
    CCTAGCAATC GCTTTAAAAC AGACTTCCTC TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT
    GCCTATGACA ACGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAT
    ACCAAATACC ATGAGCGGCT GCTGACTGGC CTCAAGGGCA GCAAAAGGCT CATTTTCATC
    GACTGTCCTG GGAAGCTGGC TGAACATATA GAGCATGAGC AACAGAAACT CCCTGCTGCT
    ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT TTCCACAATG CTCTCAAGTT AGCTCACAAG
    GACACCAAAG TTTCTATTAA GGTTGGTTCT ACTGCTGTTC AGGTAACTTC AGCAGAAAGA
    ACAAAAGTTT TGGGGCAATC CGTCTTTTTA AATGATATTT ACTATGCTTC TGAAATCGAA
    GAGATCTGCC TAGTTGATGA GAACCAGTTC ACTCTAACCA TTGCAAACCA AGGCACACCA
    CTGACCTTCA TGCACCAAGA ATGTGAAGCC ATTGTTCAGT CTATCATTCA TATCAGGACC
    CGCTGGGAGC TGTCACAACC TGACTCCATC CCTCAGCATA CCAAGATTCG ACCAAAGGAT
    GTCCCTGGGA CACTGCTTAA TATTGCGTTA CTGAATTTGG GCAGTTCAGA CCCTAGTTTA
    CGGTCTGCTG CCTATAATCT CCTGTGTGCC TTAACCTGCA CCTTTAATTT AAAGATTGAA
    GGTCAGTTGC TAGAGACATC GGGGTTATGC ATCCCTGCCA ACAACACACT CTTCATTGTC
    TCTATCAGTA AGACGCTCGC AGCCAATGAG CCACACCTCA CCTTAGAGTT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAATTGAAGC ACCTTTGTTT GGAATATATG
    ACCCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCATA ATGATGATGC CAAACGACAA
    AGGGTTACTG CCATCCTTGA TAAGCTAATA ACAATGACTA TAAACGAGAA GCAGATGTAT
    CCTTCTATTC AAGCAAAAAT CTGGGGGAGC CTTGGGCAGA TCACAGACCT GCTGGATGTT
    GTGCTTGACA GTTTCATCAA GACCAGTGCG ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG
    GTGATGGCAG ACACAGCTGT GGCTTTAGCT TCTGGAAATG TGAAATTGGT GTCGAGTAAG
    GTTATTGGAA GGATGTGTAA AATAATTGAC AAGACTTGCT TATCCCCAAC TCCAACTTTA
    GAACAACATC TTATGTGGGA CGACATTGCC ATTTTAGCCC GCTACATGCT GATGCTGTCC
    TTCAACAACT CCCTCGATGT GGCGGCTCAT CTGCCCTATC TCTTCCATGT TGTCACTTTC
    TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA GCTTCCACAC ATGGGCTGCT CATCAATATC
    ATTCACTCTC TGTGTACTTG TTCTCAGCTT CACTTTAGTG AAGAGACCAA GCAAGTTTTG
    AGGCTCAGTC TAACAGAGTT CTCGTTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT TCCAGTTACC GGGACCGCTC CTTCTCCCCT
    GGCTCCTATG AGAGGGAGAC TTTTGCTTTG ACGTCCCTGG AAACAGTCAC AGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGATT TGCGTTTCAG TATAACCCAT CGCTGCAGCC AAGAGCTCTT
    GTGGTGTTTG GCTGTATTAG CAAACGAGTG TCTCATGGGC AGATAAAGCA GATTATCCGA
    ATTCTTAGCA AGGCACTTGA AAGTTGTTTA AAAGGACCTG ACACTTACAA CAGTCAAGTT
    CTGATAGAAT CTACGGTGAT AGCACTAACA AAATTACAGC CGCTTCTTAA TAAGGACTCG
    CCCCTGCACA AAGCCCTCTT TTGGGTTGCT GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC
    TTGTATTCAG CCGGCACTGC ACTTCTGGAA CAAAACCTGC ACACCTTGGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC GGAAGAAGTA TTTATGGCGA TCCGGAATCC TCTGGAATGG
    CACTGCAAGC AGATGGATCA CTTTGTCGGA CTCAACTTCA ACTCTAACTT TAACTTTGCA
    CTAGTTGGAC ACCTCTTAAA AGGGTACAGG CATCCTTCAC CTGCCATTGT TGCAAGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTC GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CACAGAGTGT GGCTTACTTA GCAGCTCTAC TCACAGTGTC TGAAGAGGTT
    CGAAGTCGCT GTAGCCTCAA GCATAGGAAG TCCCTTCTTC TTACTGATAT TTCAATGGAA
    AATGTTCCTA TGGATACATA TCCCATTCAT CACGGTGACC CCAGCTATAG GACACTAAAG
    GAGACCCAGC CATGGTCCTC TCCCAAAGGT TCAGAAGGAT ACCTTGCAGC CACCTACCCA
    GCTGTAGGCC AAACCAGTCC CCGAGCCAGG AAATCCATGA GCTTGGATAT GGGACAGCCT
    TCTCAGGCCA ACACAAAAAA ATTGCTTGGA ACAAGAAAAA GTTTTGATCA CTTGATATCT
    GACACAAAGG CTCCTAAAAG ACAAGAAATG GAATCAGGGA TTACAACACC CCCGAAAATG
    AGAAGAGTGG CAGAGACTGA CTATGAAATG GCAGAAACCC AAAGGATTCC CTCCTCGCAG
    CAGCACCCAC ATTTACGGAA AGTCTCAGTC TCTGAATCAA ATGTTCTTTT GGATGAAGAA
    GTTCTTACCG ACCCAAAGAT CCAAGCGCTG CTTCTTACTG TTCTGGCTAC ACTGGTAAAA
    TATACTACAG ATGAATTTGA TCAACGGATT CTTTATGAAT ATCTAGCAGA GGCCAGCGTT
    GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC AATTTGTTGG ACTCTAAGAT CAATACCCTC
    TTGTCTTTAT GCCAAGATCC AAATTTGTTA AATCCAATCC ATGGAATTGT ACAGAGTGTG
    GTGTACCATG AGGAGTCCCC GCCACAGTAC CAGACATCTT ACCTGCAAAG TTTTGGTTTT
    AATGGCTTAT GGCGGTTTGC AGGACCCTTT TCAAAGCAAA CACAAATCCC TGACTATGCT
    GAGCTCATTG TTAAATTTCT TGATGCCTTG ATTGACACGT ACTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ACCCTCCTGC ACTGCAGAGC
    CAGCTTAGTA TCACTGCCAA CCTTAACCTC TCTAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGACTCGA CAAGGAGAAC GTTGAACTCT CCCCCACCGC TGGCCACTGT
    AACAGTGGAC GGACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45704
    Clone ID Related Accession (Same CDS sequence) XM_006532438.3 , XM_006532438.4
    Accession Version XM_006532438.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532438.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAG AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGCTTCCCT CAGAACAGCA TCGGTGCCGT AGGAAGTGCC
    ATGTTTCTCA GATTCATCAA TCCTGCCATT GTCTCGCCGT ATGAAGCAGG GATTTTAGAT
    AAAAAGCCAC CACCTAGAAT TGAAAGGGGC TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC
    ATTGCCAATC ATGTACTGTT CACAAAAGAA GAGCATATGA GACCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGACTTGGC ACGAAGGTTT TTCCTAGATA TAGCATCAGA TTGTCCCACA
    AGTGATGCAG TAAACCATAG TCTTTCCTTC ATCAGCGATG GCAATGTGCT TGCTTTACAT
    CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGTAA CAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCACAC TTCTCGCGTA TCTGGGTCCT
    CCGGAGCACA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTTGAGGAAT TTATGACCAG GCACCAGGTA CATGAGAAAG AAGAGTTCAA GGCCTTGAAA
    ACGTTAAGCA TCTTCTACCA AGCTGGCACC TCCAAAGCTG GGAATCCTAT TTTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGCCAGATC AATGGTGATC TGCTGATATA CCATGTCTTG
    CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT
    GGGCCTAGCA ATCGCTTTAA AACAGACTTC CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC
    TTTGCCTATG ACAACGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TATACCAAAT ACCATGAGCG GCTGCTGACT GGCCTCAAGG GCAGCAAAAG GCTCATTTTC
    ATCGACTGTC CTGGGAAGCT GGCTGAACAT ATAGAGCATG AGCAACAGAA ACTCCCTGCT
    GCTACCTTGG CTTTGGAAGA AGATCTAAAG GTTTTCCACA ATGCTCTCAA GTTAGCTCAC
    AAGGACACCA AAGTTTCTAT TAAGGTTGGT TCTACTGCTG TTCAGGTAAC TTCAGCAGAA
    AGAACAAAAG TTTTGGGGCA ATCCGTCTTT TTAAATGATA TTTACTATGC TTCTGAAATC
    GAAGAGATCT GCCTAGTTGA TGAGAACCAG TTCACTCTAA CCATTGCAAA CCAAGGCACA
    CCACTGACCT TCATGCACCA AGAATGTGAA GCCATTGTTC AGTCTATCAT TCATATCAGG
    ACCCGCTGGG AGCTGTCACA ACCTGACTCC ATCCCTCAGC ATACCAAGAT TCGACCAAAG
    GATGTCCCTG GGACACTGCT TAATATTGCG TTACTGAATT TGGGCAGTTC AGACCCTAGT
    TTACGGTCTG CTGCCTATAA TCTCCTGTGT GCCTTAACCT GCACCTTTAA TTTAAAGATT
    GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA TGCATCCCTG CCAACAACAC ACTCTTCATT
    GTCTCTATCA GTAAGACGCT CGCAGCCAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT
    ATGACCCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ATAATGATGA TGCCAAACGA
    CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CTATAAACGA GAAGCAGATG
    TATCCTTCTA TTCAAGCAAA AATCTGGGGG AGCCTTGGGC AGATCACAGA CCTGCTGGAT
    GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCGACAGGAG GCTTAGGGTC TATCAAAGCT
    GAGGTGATGG CAGACACAGC TGTGGCTTTA GCTTCTGGAA ATGTGAAATT GGTGTCGAGT
    AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCCCC AACTCCAACT
    TTAGAACAAC ATCTTATGTG GGACGACATT GCCATTTTAG CCCGCTACAT GCTGATGCTG
    TCCTTCAACA ACTCCCTCGA TGTGGCGGCT CATCTGCCCT ATCTCTTCCA TGTTGTCACT
    TTCTTAGTAG CCACAGGTCC CTTGTCCCTC CGAGCTTCCA CACATGGGCT GCTCATCAAT
    ATCATTCACT CTCTGTGTAC TTGTTCTCAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT
    TTGAGGCTCA GTCTAACAGA GTTCTCGTTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CGGCTGCTGT CATTGCCTTC CGTTCCAGTT ACCGGGACCG CTCCTTCTCC
    CCTGGCTCCT ATGAGAGGGA GACTTTTGCT TTGACGTCCC TGGAAACAGT CACAGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG ATTTGCGTTT CAGTATAACC CATCGCTGCA GCCAAGAGCT
    CTTGTGGTGT TTGGCTGTAT TAGCAAACGA GTGTCTCATG GGCAGATAAA GCAGATTATC
    CGAATTCTTA GCAAGGCACT TGAAAGTTGT TTAAAAGGAC CTGACACTTA CAACAGTCAA
    GTTCTGATAG AATCTACGGT GATAGCACTA ACAAAATTAC AGCCGCTTCT TAATAAGGAC
    TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT GCTGTGGCTG TGCTGCAGCT GGACGAAGTC
    AACTTGTATT CAGCCGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT GGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCGGAAGAA GTATTTATGG CGATCCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAGATGGA TCACTTTGTC GGACTCAACT TCAACTCTAA CTTTAACTTT
    GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTCGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG
    GTTCGAAGTC GCTGTAGCCT CAAGCATAGG AAGTCCCTTC TTCTTACTGA TATTTCAATG
    GAAAATGTTC CTATGGATAC ATATCCCATT CATCACGGTG ACCCCAGCTA TAGGACACTA
    AAGGAGACCC AGCCATGGTC CTCTCCCAAA GGTTCAGAAG GATACCTTGC AGCCACCTAC
    CCAGCTGTAG GCCAAACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA TATGGGACAG
    CCTTCTCAGG CCAACACAAA AAAATTGCTT GGAACAAGAA AAAGTTTTGA TCACTTGATA
    TCTGACACAA AGGCTCCTAA AAGACAAGAA ATGGAATCAG GGATTACAAC ACCCCCGAAA
    ATGAGAAGAG TGGCAGAGAC TGACTATGAA ATGGAAACCC AAAGGATTCC CTCCTCGCAG
    CAGCACCCAC ATTTACGGAA AGTCTCAGTC TCTGAATCAA ATGTTCTTTT GGATGAAGAA
    GTTCTTACCG ACCCAAAGAT CCAAGCGCTG CTTCTTACTG TTCTGGCTAC ACTGGTAAAA
    TATACTACAG ATGAATTTGA TCAACGGATT CTTTATGAAT ATCTAGCAGA GGCCAGCGTT
    GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC AATTTGTTGG ACTCTAAGAT CAATACCCTC
    TTGTCTTTAT GCCAAGATCC AAATTTGTTA AATCCAATCC ATGGAATTGT ACAGAGTGTG
    GTGTACCATG AGGAGTCCCC GCCACAGTAC CAGACATCTT ACCTGCAAAG TTTTGGTTTT
    AATGGCTTAT GGCGGTTTGC AGGACCCTTT TCAAAGCAAA CACAAATCCC TGACTATGCT
    GAGCTCATTG TTAAATTTCT TGATGCCTTG ATTGACACGT ACTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ACCCTCCTGC ACTGCAGAGC
    CAGCTTAGTA TCACTGCCAA CCTTAACCTC TCTAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGACTCGA CAAGGAGAAC GTTGAACTCT CCCCCACCGC TGGCCACTGT
    AACAGTGGAC GGACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532501.1
    CDS1612..10167
    Translation

    Target ORF information:

    RefSeq Version XM_006532438.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromatosis 1 (Nf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532438.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAG AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGCTTCCCT CAGAACAGCA TCGGTGCCGT AGGAAGTGCC
    ATGTTTCTCA GATTCATCAA TCCTGCCATT GTCTCGCCGT ATGAAGCAGG GATTTTAGAT
    AAAAAGCCAC CACCTAGAAT TGAAAGGGGC TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC
    ATTGCCAATC ATGTACTGTT CACAAAAGAA GAGCATATGA GACCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGACTTGGC ACGAAGGTTT TTCCTAGATA TAGCATCAGA TTGTCCCACA
    AGTGATGCAG TAAACCATAG TCTTTCCTTC ATCAGCGATG GCAATGTGCT TGCTTTACAT
    CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGTAA CAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCACAC TTCTCGCGTA TCTGGGTCCT
    CCGGAGCACA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTTGAGGAAT TTATGACCAG GCACCAGGTA CATGAGAAAG AAGAGTTCAA GGCCTTGAAA
    ACGTTAAGCA TCTTCTACCA AGCTGGCACC TCCAAAGCTG GGAATCCTAT TTTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGCCAGATC AATGGTGATC TGCTGATATA CCATGTCTTG
    CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT
    GGGCCTAGCA ATCGCTTTAA AACAGACTTC CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC
    TTTGCCTATG ACAACGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TATACCAAAT ACCATGAGCG GCTGCTGACT GGCCTCAAGG GCAGCAAAAG GCTCATTTTC
    ATCGACTGTC CTGGGAAGCT GGCTGAACAT ATAGAGCATG AGCAACAGAA ACTCCCTGCT
    GCTACCTTGG CTTTGGAAGA AGATCTAAAG GTTTTCCACA ATGCTCTCAA GTTAGCTCAC
    AAGGACACCA AAGTTTCTAT TAAGGTTGGT TCTACTGCTG TTCAGGTAAC TTCAGCAGAA
    AGAACAAAAG TTTTGGGGCA ATCCGTCTTT TTAAATGATA TTTACTATGC TTCTGAAATC
    GAAGAGATCT GCCTAGTTGA TGAGAACCAG TTCACTCTAA CCATTGCAAA CCAAGGCACA
    CCACTGACCT TCATGCACCA AGAATGTGAA GCCATTGTTC AGTCTATCAT TCATATCAGG
    ACCCGCTGGG AGCTGTCACA ACCTGACTCC ATCCCTCAGC ATACCAAGAT TCGACCAAAG
    GATGTCCCTG GGACACTGCT TAATATTGCG TTACTGAATT TGGGCAGTTC AGACCCTAGT
    TTACGGTCTG CTGCCTATAA TCTCCTGTGT GCCTTAACCT GCACCTTTAA TTTAAAGATT
    GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA TGCATCCCTG CCAACAACAC ACTCTTCATT
    GTCTCTATCA GTAAGACGCT CGCAGCCAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT
    ATGACCCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ATAATGATGA TGCCAAACGA
    CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CTATAAACGA GAAGCAGATG
    TATCCTTCTA TTCAAGCAAA AATCTGGGGG AGCCTTGGGC AGATCACAGA CCTGCTGGAT
    GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCGACAGGAG GCTTAGGGTC TATCAAAGCT
    GAGGTGATGG CAGACACAGC TGTGGCTTTA GCTTCTGGAA ATGTGAAATT GGTGTCGAGT
    AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCCCC AACTCCAACT
    TTAGAACAAC ATCTTATGTG GGACGACATT GCCATTTTAG CCCGCTACAT GCTGATGCTG
    TCCTTCAACA ACTCCCTCGA TGTGGCGGCT CATCTGCCCT ATCTCTTCCA TGTTGTCACT
    TTCTTAGTAG CCACAGGTCC CTTGTCCCTC CGAGCTTCCA CACATGGGCT GCTCATCAAT
    ATCATTCACT CTCTGTGTAC TTGTTCTCAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT
    TTGAGGCTCA GTCTAACAGA GTTCTCGTTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CGGCTGCTGT CATTGCCTTC CGTTCCAGTT ACCGGGACCG CTCCTTCTCC
    CCTGGCTCCT ATGAGAGGGA GACTTTTGCT TTGACGTCCC TGGAAACAGT CACAGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG ATTTGCGTTT CAGTATAACC CATCGCTGCA GCCAAGAGCT
    CTTGTGGTGT TTGGCTGTAT TAGCAAACGA GTGTCTCATG GGCAGATAAA GCAGATTATC
    CGAATTCTTA GCAAGGCACT TGAAAGTTGT TTAAAAGGAC CTGACACTTA CAACAGTCAA
    GTTCTGATAG AATCTACGGT GATAGCACTA ACAAAATTAC AGCCGCTTCT TAATAAGGAC
    TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT GCTGTGGCTG TGCTGCAGCT GGACGAAGTC
    AACTTGTATT CAGCCGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT GGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCGGAAGAA GTATTTATGG CGATCCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAGATGGA TCACTTTGTC GGACTCAACT TCAACTCTAA CTTTAACTTT
    GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTCGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG
    GTTCGAAGTC GCTGTAGCCT CAAGCATAGG AAGTCCCTTC TTCTTACTGA TATTTCAATG
    GAAAATGTTC CTATGGATAC ATATCCCATT CATCACGGTG ACCCCAGCTA TAGGACACTA
    AAGGAGACCC AGCCATGGTC CTCTCCCAAA GGTTCAGAAG GATACCTTGC AGCCACCTAC
    CCAGCTGTAG GCCAAACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA TATGGGACAG
    CCTTCTCAGG CCAACACAAA AAAATTGCTT GGAACAAGAA AAAGTTTTGA TCACTTGATA
    TCTGACACAA AGGCTCCTAA AAGACAAGAA ATGGAATCAG GGATTACAAC ACCCCCGAAA
    ATGAGAAGAG TGGCAGAGAC TGACTATGAA ATGGAAACCC AAAGGATTCC CTCCTCGCAG
    CAGCACCCAC ATTTACGGAA AGTCTCAGTC TCTGAATCAA ATGTTCTTTT GGATGAAGAA
    GTTCTTACCG ACCCAAAGAT CCAAGCGCTG CTTCTTACTG TTCTGGCTAC ACTGGTAAAA
    TATACTACAG ATGAATTTGA TCAACGGATT CTTTATGAAT ATCTAGCAGA GGCCAGCGTT
    GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC AATTTGTTGG ACTCTAAGAT CAATACCCTC
    TTGTCTTTAT GCCAAGATCC AAATTTGTTA AATCCAATCC ATGGAATTGT ACAGAGTGTG
    GTGTACCATG AGGAGTCCCC GCCACAGTAC CAGACATCTT ACCTGCAAAG TTTTGGTTTT
    AATGGCTTAT GGCGGTTTGC AGGACCCTTT TCAAAGCAAA CACAAATCCC TGACTATGCT
    GAGCTCATTG TTAAATTTCT TGATGCCTTG ATTGACACGT ACTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ACCCTCCTGC ACTGCAGAGC
    CAGCTTAGTA TCACTGCCAA CCTTAACCTC TCTAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGACTCGA CAAGGAGAAC GTTGAACTCT CCCCCACCGC TGGCCACTGT
    AACAGTGGAC GGACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45704
    Clone ID Related Accession (Same CDS sequence) XM_006532438.3 , XM_006532438.4
    Accession Version XM_006532438.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532438.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
    181
    241
    301
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    481
    541
    601
    661
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    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
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    2641
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    3181
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    4141
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    4681
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    6001
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    6181
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    6301
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    6421
    6481
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    7141
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    7621
    7681
    7741
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    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAG AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGCTTCCCT CAGAACAGCA TCGGTGCCGT AGGAAGTGCC
    ATGTTTCTCA GATTCATCAA TCCTGCCATT GTCTCGCCGT ATGAAGCAGG GATTTTAGAT
    AAAAAGCCAC CACCTAGAAT TGAAAGGGGC TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC
    ATTGCCAATC ATGTACTGTT CACAAAAGAA GAGCATATGA GACCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGACTTGGC ACGAAGGTTT TTCCTAGATA TAGCATCAGA TTGTCCCACA
    AGTGATGCAG TAAACCATAG TCTTTCCTTC ATCAGCGATG GCAATGTGCT TGCTTTACAT
    CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGTAA CAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCACAC TTCTCGCGTA TCTGGGTCCT
    CCGGAGCACA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTTGAGGAAT TTATGACCAG GCACCAGGTA CATGAGAAAG AAGAGTTCAA GGCCTTGAAA
    ACGTTAAGCA TCTTCTACCA AGCTGGCACC TCCAAAGCTG GGAATCCTAT TTTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGCCAGATC AATGGTGATC TGCTGATATA CCATGTCTTG
    CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT
    GGGCCTAGCA ATCGCTTTAA AACAGACTTC CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC
    TTTGCCTATG ACAACGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TATACCAAAT ACCATGAGCG GCTGCTGACT GGCCTCAAGG GCAGCAAAAG GCTCATTTTC
    ATCGACTGTC CTGGGAAGCT GGCTGAACAT ATAGAGCATG AGCAACAGAA ACTCCCTGCT
    GCTACCTTGG CTTTGGAAGA AGATCTAAAG GTTTTCCACA ATGCTCTCAA GTTAGCTCAC
    AAGGACACCA AAGTTTCTAT TAAGGTTGGT TCTACTGCTG TTCAGGTAAC TTCAGCAGAA
    AGAACAAAAG TTTTGGGGCA ATCCGTCTTT TTAAATGATA TTTACTATGC TTCTGAAATC
    GAAGAGATCT GCCTAGTTGA TGAGAACCAG TTCACTCTAA CCATTGCAAA CCAAGGCACA
    CCACTGACCT TCATGCACCA AGAATGTGAA GCCATTGTTC AGTCTATCAT TCATATCAGG
    ACCCGCTGGG AGCTGTCACA ACCTGACTCC ATCCCTCAGC ATACCAAGAT TCGACCAAAG
    GATGTCCCTG GGACACTGCT TAATATTGCG TTACTGAATT TGGGCAGTTC AGACCCTAGT
    TTACGGTCTG CTGCCTATAA TCTCCTGTGT GCCTTAACCT GCACCTTTAA TTTAAAGATT
    GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA TGCATCCCTG CCAACAACAC ACTCTTCATT
    GTCTCTATCA GTAAGACGCT CGCAGCCAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT
    ATGACCCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ATAATGATGA TGCCAAACGA
    CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CTATAAACGA GAAGCAGATG
    TATCCTTCTA TTCAAGCAAA AATCTGGGGG AGCCTTGGGC AGATCACAGA CCTGCTGGAT
    GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCGACAGGAG GCTTAGGGTC TATCAAAGCT
    GAGGTGATGG CAGACACAGC TGTGGCTTTA GCTTCTGGAA ATGTGAAATT GGTGTCGAGT
    AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCCCC AACTCCAACT
    TTAGAACAAC ATCTTATGTG GGACGACATT GCCATTTTAG CCCGCTACAT GCTGATGCTG
    TCCTTCAACA ACTCCCTCGA TGTGGCGGCT CATCTGCCCT ATCTCTTCCA TGTTGTCACT
    TTCTTAGTAG CCACAGGTCC CTTGTCCCTC CGAGCTTCCA CACATGGGCT GCTCATCAAT
    ATCATTCACT CTCTGTGTAC TTGTTCTCAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT
    TTGAGGCTCA GTCTAACAGA GTTCTCGTTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CGGCTGCTGT CATTGCCTTC CGTTCCAGTT ACCGGGACCG CTCCTTCTCC
    CCTGGCTCCT ATGAGAGGGA GACTTTTGCT TTGACGTCCC TGGAAACAGT CACAGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG ATTTGCGTTT CAGTATAACC CATCGCTGCA GCCAAGAGCT
    CTTGTGGTGT TTGGCTGTAT TAGCAAACGA GTGTCTCATG GGCAGATAAA GCAGATTATC
    CGAATTCTTA GCAAGGCACT TGAAAGTTGT TTAAAAGGAC CTGACACTTA CAACAGTCAA
    GTTCTGATAG AATCTACGGT GATAGCACTA ACAAAATTAC AGCCGCTTCT TAATAAGGAC
    TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT GCTGTGGCTG TGCTGCAGCT GGACGAAGTC
    AACTTGTATT CAGCCGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT GGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCGGAAGAA GTATTTATGG CGATCCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAGATGGA TCACTTTGTC GGACTCAACT TCAACTCTAA CTTTAACTTT
    GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTCGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG
    GTTCGAAGTC GCTGTAGCCT CAAGCATAGG AAGTCCCTTC TTCTTACTGA TATTTCAATG
    GAAAATGTTC CTATGGATAC ATATCCCATT CATCACGGTG ACCCCAGCTA TAGGACACTA
    AAGGAGACCC AGCCATGGTC CTCTCCCAAA GGTTCAGAAG GATACCTTGC AGCCACCTAC
    CCAGCTGTAG GCCAAACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA TATGGGACAG
    CCTTCTCAGG CCAACACAAA AAAATTGCTT GGAACAAGAA AAAGTTTTGA TCACTTGATA
    TCTGACACAA AGGCTCCTAA AAGACAAGAA ATGGAATCAG GGATTACAAC ACCCCCGAAA
    ATGAGAAGAG TGGCAGAGAC TGACTATGAA ATGGAAACCC AAAGGATTCC CTCCTCGCAG
    CAGCACCCAC ATTTACGGAA AGTCTCAGTC TCTGAATCAA ATGTTCTTTT GGATGAAGAA
    GTTCTTACCG ACCCAAAGAT CCAAGCGCTG CTTCTTACTG TTCTGGCTAC ACTGGTAAAA
    TATACTACAG ATGAATTTGA TCAACGGATT CTTTATGAAT ATCTAGCAGA GGCCAGCGTT
    GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC AATTTGTTGG ACTCTAAGAT CAATACCCTC
    TTGTCTTTAT GCCAAGATCC AAATTTGTTA AATCCAATCC ATGGAATTGT ACAGAGTGTG
    GTGTACCATG AGGAGTCCCC GCCACAGTAC CAGACATCTT ACCTGCAAAG TTTTGGTTTT
    AATGGCTTAT GGCGGTTTGC AGGACCCTTT TCAAAGCAAA CACAAATCCC TGACTATGCT
    GAGCTCATTG TTAAATTTCT TGATGCCTTG ATTGACACGT ACTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ACCCTCCTGC ACTGCAGAGC
    CAGCTTAGTA TCACTGCCAA CCTTAACCTC TCTAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGACTCGA CAAGGAGAAC GTTGAACTCT CCCCCACCGC TGGCCACTGT
    AACAGTGGAC GGACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532501.1
    CDS407..8962
    Translation

    Target ORF information:

    RefSeq Version XM_006532438.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromin 1 (Nf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532438.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAG AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGCTTCCCT CAGAACAGCA TCGGTGCCGT AGGAAGTGCC
    ATGTTTCTCA GATTCATCAA TCCTGCCATT GTCTCGCCGT ATGAAGCAGG GATTTTAGAT
    AAAAAGCCAC CACCTAGAAT TGAAAGGGGC TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC
    ATTGCCAATC ATGTACTGTT CACAAAAGAA GAGCATATGA GACCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGACTTGGC ACGAAGGTTT TTCCTAGATA TAGCATCAGA TTGTCCCACA
    AGTGATGCAG TAAACCATAG TCTTTCCTTC ATCAGCGATG GCAATGTGCT TGCTTTACAT
    CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGTAA CAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCACAC TTCTCGCGTA TCTGGGTCCT
    CCGGAGCACA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTTGAGGAAT TTATGACCAG GCACCAGGTA CATGAGAAAG AAGAGTTCAA GGCCTTGAAA
    ACGTTAAGCA TCTTCTACCA AGCTGGCACC TCCAAAGCTG GGAATCCTAT TTTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGCCAGATC AATGGTGATC TGCTGATATA CCATGTCTTG
    CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT
    GGGCCTAGCA ATCGCTTTAA AACAGACTTC CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC
    TTTGCCTATG ACAACGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TATACCAAAT ACCATGAGCG GCTGCTGACT GGCCTCAAGG GCAGCAAAAG GCTCATTTTC
    ATCGACTGTC CTGGGAAGCT GGCTGAACAT ATAGAGCATG AGCAACAGAA ACTCCCTGCT
    GCTACCTTGG CTTTGGAAGA AGATCTAAAG GTTTTCCACA ATGCTCTCAA GTTAGCTCAC
    AAGGACACCA AAGTTTCTAT TAAGGTTGGT TCTACTGCTG TTCAGGTAAC TTCAGCAGAA
    AGAACAAAAG TTTTGGGGCA ATCCGTCTTT TTAAATGATA TTTACTATGC TTCTGAAATC
    GAAGAGATCT GCCTAGTTGA TGAGAACCAG TTCACTCTAA CCATTGCAAA CCAAGGCACA
    CCACTGACCT TCATGCACCA AGAATGTGAA GCCATTGTTC AGTCTATCAT TCATATCAGG
    ACCCGCTGGG AGCTGTCACA ACCTGACTCC ATCCCTCAGC ATACCAAGAT TCGACCAAAG
    GATGTCCCTG GGACACTGCT TAATATTGCG TTACTGAATT TGGGCAGTTC AGACCCTAGT
    TTACGGTCTG CTGCCTATAA TCTCCTGTGT GCCTTAACCT GCACCTTTAA TTTAAAGATT
    GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA TGCATCCCTG CCAACAACAC ACTCTTCATT
    GTCTCTATCA GTAAGACGCT CGCAGCCAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT
    ATGACCCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ATAATGATGA TGCCAAACGA
    CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CTATAAACGA GAAGCAGATG
    TATCCTTCTA TTCAAGCAAA AATCTGGGGG AGCCTTGGGC AGATCACAGA CCTGCTGGAT
    GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCGACAGGAG GCTTAGGGTC TATCAAAGCT
    GAGGTGATGG CAGACACAGC TGTGGCTTTA GCTTCTGGAA ATGTGAAATT GGTGTCGAGT
    AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCCCC AACTCCAACT
    TTAGAACAAC ATCTTATGTG GGACGACATT GCCATTTTAG CCCGCTACAT GCTGATGCTG
    TCCTTCAACA ACTCCCTCGA TGTGGCGGCT CATCTGCCCT ATCTCTTCCA TGTTGTCACT
    TTCTTAGTAG CCACAGGTCC CTTGTCCCTC CGAGCTTCCA CACATGGGCT GCTCATCAAT
    ATCATTCACT CTCTGTGTAC TTGTTCTCAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT
    TTGAGGCTCA GTCTAACAGA GTTCTCGTTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CGGCTGCTGT CATTGCCTTC CGTTCCAGTT ACCGGGACCG CTCCTTCTCC
    CCTGGCTCCT ATGAGAGGGA GACTTTTGCT TTGACGTCCC TGGAAACAGT CACAGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG ATTTGCGTTT CAGTATAACC CATCGCTGCA GCCAAGAGCT
    CTTGTGGTGT TTGGCTGTAT TAGCAAACGA GTGTCTCATG GGCAGATAAA GCAGATTATC
    CGAATTCTTA GCAAGGCACT TGAAAGTTGT TTAAAAGGAC CTGACACTTA CAACAGTCAA
    GTTCTGATAG AATCTACGGT GATAGCACTA ACAAAATTAC AGCCGCTTCT TAATAAGGAC
    TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT GCTGTGGCTG TGCTGCAGCT GGACGAAGTC
    AACTTGTATT CAGCCGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT GGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCGGAAGAA GTATTTATGG CGATCCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAGATGGA TCACTTTGTC GGACTCAACT TCAACTCTAA CTTTAACTTT
    GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTCGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG
    GTTCGAAGTC GCTGTAGCCT CAAGCATAGG AAGTCCCTTC TTCTTACTGA TATTTCAATG
    GAAAATGTTC CTATGGATAC ATATCCCATT CATCACGGTG ACCCCAGCTA TAGGACACTA
    AAGGAGACCC AGCCATGGTC CTCTCCCAAA GGTTCAGAAG GATACCTTGC AGCCACCTAC
    CCAGCTGTAG GCCAAACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA TATGGGACAG
    CCTTCTCAGG CCAACACAAA AAAATTGCTT GGAACAAGAA AAAGTTTTGA TCACTTGATA
    TCTGACACAA AGGCTCCTAA AAGACAAGAA ATGGAATCAG GGATTACAAC ACCCCCGAAA
    ATGAGAAGAG TGGCAGAGAC TGACTATGAA ATGGAAACCC AAAGGATTCC CTCCTCGCAG
    CAGCACCCAC ATTTACGGAA AGTCTCAGTC TCTGAATCAA ATGTTCTTTT GGATGAAGAA
    GTTCTTACCG ACCCAAAGAT CCAAGCGCTG CTTCTTACTG TTCTGGCTAC ACTGGTAAAA
    TATACTACAG ATGAATTTGA TCAACGGATT CTTTATGAAT ATCTAGCAGA GGCCAGCGTT
    GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC AATTTGTTGG ACTCTAAGAT CAATACCCTC
    TTGTCTTTAT GCCAAGATCC AAATTTGTTA AATCCAATCC ATGGAATTGT ACAGAGTGTG
    GTGTACCATG AGGAGTCCCC GCCACAGTAC CAGACATCTT ACCTGCAAAG TTTTGGTTTT
    AATGGCTTAT GGCGGTTTGC AGGACCCTTT TCAAAGCAAA CACAAATCCC TGACTATGCT
    GAGCTCATTG TTAAATTTCT TGATGCCTTG ATTGACACGT ACTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ACCCTCCTGC ACTGCAGAGC
    CAGCTTAGTA TCACTGCCAA CCTTAACCTC TCTAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGACTCGA CAAGGAGAAC GTTGAACTCT CCCCCACCGC TGGCCACTGT
    AACAGTGGAC GGACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45705
    Clone ID Related Accession (Same CDS sequence) XM_006532439.3 , XM_006532439.4
    Accession Version XM_006532439.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8529bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532439.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGC
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGCT GTGGAGCGCA CCCAGCAATA
    CGAATGGCAC CAAGTCTCAC CTTTAAAGAA AAGGTAACAA GCCTTAAATT TAAAGAAAAG
    CCTACAGACC TAGAGACAAG GAGCTACAAA TGTCTTCTTT TGTCCATGGT GAAACTGATT
    CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAGAGC
    AGTACAGCAG AACTAATTAC AGGGCTCGTA CAACTGGTTC CTCAGTCACA CATGCCAGAG
    GTCGCTCAGG AGGCTATGGA GGCTTTGCTG GTCCTTCATC AGTTAGATAG CATTGATCTC
    TGGAATCCTG ACGCTCCTGT AGAGACATTC TGGGAAATCA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA
    CGGGAAATTC TGATCTGCAG AAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGC
    TCCTGTCATT CTCTCTACCT TTATGGAGTA GGATGTGAAA TGTCTGCTAC TGGAAATACC
    ACTCAGATGT CAGTTGATCA TGATGAGTTC CTGCGTGCCT GTACTCCTGG AGCATCTCTG
    CGGAAAGGAA GAGGGAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCT
    ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCCTTAT ACATGTTTCT GTGGAATCCT
    GACACTGAAG CTGTTCTGGT TGCTATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT
    ATTCGATGTG GGGTAGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CCTCAGTCAG CAATATGATG TCAACAGGAA GAGCAGCGCT TCAGAAAAGA
    GTGATGGCCC TGCTAAGGCG CATTGAGCAC CCTACTGCAG GAAACATTGA GGCCTGGGAA
    GATACACATG CAAAGTGGGA ACAGGCTACA AAACTAATCC TTAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC AGAAAGTCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC
    CATGTCAGTG GAGGAGGCTC CATAGACTTG TCTGACACAG ACTCCCTGCA GGAGTGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT
    GGCCTAGCGA CATACAGCCC ACCTATGGGC GCTGTCAGTG AACGCAAAGG GTCTATGATT
    TCTGTAATGT CTTCTGAAGG GAATATTGAT TCACCTGTCA GCAGATTTAT GGACCGGCTT
    CTGTCCTTAA TGGTGTGTAA CCACGAGAAA GTGGGGCTTC AGATACGGAC CAATGTTAAG
    GACCTGGTGG GTCTGGAGTT GAGTCCTGCT CTGTATCCAA TGCTGTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TACTAAGTGA CAGCAATACT
    CAGTTTGTAG AGCAAACCAT AGCCATAATG AAAAACCTAC TGGATAATCA TACTGAAGGC
    AGCTCTGAGC ATCTGGGACA AGCGAGCATT GAGACAATGA TGCTAAATCT GGTCAGATAC
    GTTCGTGTTC TTGGGAATAT GGTCCATGCA ATACAAATAA AAACAAAATT GTGTCAGTTA
    GTTGAAGTCA TGATGGCAAG AAGAGATGAC CTCTCGTTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT AACAGACTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGACATAA AATGTCTTAC GAGAGATCTG GACCAAGCAA GCATGGAAGC AGTAGTTTCT
    CTCCTCGCTG GTCTTCCACT GCAGCCCGAA GAAGGAGATG GGGTGGAGCT GATGGAGGCC
    AAATCACAGT TATTCCTTAA GTACTTCACA TTATTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACGGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCCCTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC
    AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCACAAGG ATCTTCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTCGATAC ACTTGCGGAA
    ACGGTGTTGG CAGATCGATT TGAGAGACTT GTGGAACTGG TCACAATGAT GGGAGACCAG
    GGAGAACTCC CTATAGCTAT GGCTCTTGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG
    CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT TCCCGGCATT TACTCTACCA GCTGCTCTGG
    AACATGTTTT CTAAGGAGGT AGAATTGGCA GACTCCATGC AGACTCTCTT TCGAGGCAAC
    AGCTTGGCCA GTAAGATAAT GACATTCTGC TTCAAGGTGT ATGGTGCTAC TTACCTACAA
    AAGCTCTTGG ACCCTTTATT ACGAGTCATC ATCACATCTT CGGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACCC TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC
    CTCCTTCAGA TGACAGAGAA GTTCTTCCAT GCCATCATCA GCTCTTCCTC AGAATTCCCC
    TCACAGCTTC GAAGTGTCTG CCATTGTTTG TACCAGGCAA CTTGCCACTC CCTACTGAAT
    AAAGCTACAG TAAAAGAAAG AAAGGAAAAC AAAAAATCAG TGGTTAGCCA GCGCTTCCCT
    CAGAACAGCA TCGGTGCCGT AGGAAGTGCC ATGTTTCTCA GATTCATCAA TCCTGCCATT
    GTCTCGCCGT ATGAAGCAGG GATTTTAGAT AAAAAGCCAC CACCTAGAAT TGAAAGGGGC
    TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC ATTGCCAATC ATGTACTGTT CACAAAAGAA
    GAGCATATGA GACCTTTTAA TGATTTTGTG AAAAGCAACT TTGACTTGGC ACGAAGGTTT
    TTCCTAGATA TAGCATCAGA TTGTCCCACA AGTGATGCAG TAAACCATAG TCTTTCCTTC
    ATCAGCGATG GCAATGTGCT TGCTTTACAT CGGCTGCTTT GGAACAATCA GGAGAAAATT
    GGCCAGTATC TTTCCAGTAA CAGGGATCAT AAAGCTGTTG GAAGACGACC TTTTGATAAG
    ATGGCCACAC TTCTCGCGTA TCTGGGTCCT CCGGAGCACA AGCCTGTGGC AGATACACAC
    TGGTCCAGCC TTAACCTTAC CAGTTCAAAG TTTGAGGAAT TTATGACCAG GCACCAGGTA
    CATGAGAAAG AAGAGTTCAA GGCCTTGAAA ACGTTAAGCA TCTTCTACCA AGCTGGCACC
    TCCAAAGCTG GGAATCCTAT TTTTTATTAT GTTGCACGGA GGTTCAAAAC TGGCCAGATC
    AATGGTGATC TGCTGATATA CCATGTCTTG CTGACTTTAA AGCCATATTA TGCAAAGCCA
    TATGAAATTG TAGTGGACCT TACCCATACT GGGCCTAGCA ATCGCTTTAA AACAGACTTC
    CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC TTTGCCTATG ACAACGTCTC TGCAGTCTAT
    ATCTATAACT GTAACTCCTG GGTCAGGGAG TATACCAAAT ACCATGAGCG GCTGCTGACT
    GGCCTCAAGG GCAGCAAAAG GCTCATTTTC ATCGACTGTC CTGGGAAGCT GGCTGAACAT
    ATAGAGCATG AGCAACAGAA ACTCCCTGCT GCTACCTTGG CTTTGGAAGA AGATCTAAAG
    GTTTTCCACA ATGCTCTCAA GTTAGCTCAC AAGGACACCA AAGTTTCTAT TAAGGTTGGT
    TCTACTGCTG TTCAGGTAAC TTCAGCAGAA AGAACAAAAG TTTTGGGGCA ATCCGTCTTT
    TTAAATGATA TTTACTATGC TTCTGAAATC GAAGAGATCT GCCTAGTTGA TGAGAACCAG
    TTCACTCTAA CCATTGCAAA CCAAGGCACA CCACTGACCT TCATGCACCA AGAATGTGAA
    GCCATTGTTC AGTCTATCAT TCATATCAGG ACCCGCTGGG AGCTGTCACA ACCTGACTCC
    ATCCCTCAGC ATACCAAGAT TCGACCAAAG GATGTCCCTG GGACACTGCT TAATATTGCG
    TTACTGAATT TGGGCAGTTC AGACCCTAGT TTACGGTCTG CTGCCTATAA TCTCCTGTGT
    GCCTTAACCT GCACCTTTAA TTTAAAGATT GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA
    TGCATCCCTG CCAACAACAC ACTCTTCATT GTCTCTATCA GTAAGACGCT CGCAGCCAAT
    GAGCCACACC TCACCTTAGA GTTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAGT
    ATTGAATTGA AGCACCTTTG TTTGGAATAT ATGACCCCAT GGCTGTCAAA TCTAGTCCGT
    TTTTGTAAGC ATAATGATGA TGCCAAACGA CAAAGGGTTA CTGCCATCCT TGATAAGCTA
    ATAACAATGA CTATAAACGA GAAGCAGATG TATCCTTCTA TTCAAGCAAA AATCTGGGGG
    AGCCTTGGGC AGATCACAGA CCTGCTGGAT GTTGTGCTTG ACAGTTTCAT CAAGACCAGT
    GCGACAGGAG GCTTAGGGTC TATCAAAGCT GAGGTGATGG CAGACACAGC TGTGGCTTTA
    GCTTCTGGAA ATGTGAAATT GGTGTCGAGT AAGGTTATTG GAAGGATGTG TAAAATAATT
    GACAAGACTT GCTTATCCCC AACTCCAACT TTAGAACAAC ATCTTATGTG GGACGACATT
    GCCATTTTAG CCCGCTACAT GCTGATGCTG TCCTTCAACA ACTCCCTCGA TGTGGCGGCT
    CATCTGCCCT ATCTCTTCCA TGTTGTCACT TTCTTAGTAG CCACAGGTCC CTTGTCCCTC
    CGAGCTTCCA CACATGGGCT GCTCATCAAT ATCATTCACT CTCTGTGTAC TTGTTCTCAG
    CTTCACTTTA GTGAAGAGAC CAAGCAAGTT TTGAGGCTCA GTCTAACAGA GTTCTCGTTA
    CCCAAATTTT ACTTACTGTT TGGCATTAGC AAAGTCAAGT CGGCTGCTGT CATTGCCTTC
    CGTTCCAGTT ACCGGGACCG CTCCTTCTCC CCTGGCTCCT ATGAGAGGGA GACTTTTGCT
    TTGACGTCCC TGGAAACAGT CACAGAAGCT TTGTTGGAGA TCATGGAGGC ATGTATGAGA
    GATATTCCAA CATGCAAGTG GCTGGATCAG TGGACAGAAC TAGCTCAAAG ATTTGCGTTT
    CAGTATAACC CATCGCTGCA GCCAAGAGCT CTTGTGGTGT TTGGCTGTAT TAGCAAACGA
    GTGTCTCATG GGCAGATAAA GCAGATTATC CGAATTCTTA GCAAGGCACT TGAAAGTTGT
    TTAAAAGGAC CTGACACTTA CAACAGTCAA GTTCTGATAG AATCTACGGT GATAGCACTA
    ACAAAATTAC AGCCGCTTCT TAATAAGGAC TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT
    GCTGTGGCTG TGCTGCAGCT GGACGAAGTC AACTTGTATT CAGCCGGCAC TGCACTTCTG
    GAACAAAACC TGCACACCTT GGACAGTCTC CGGATATTCA ATGACAAGAG TCCGGAAGAA
    GTATTTATGG CGATCCGGAA TCCTCTGGAA TGGCACTGCA AGCAGATGGA TCACTTTGTC
    GGACTCAACT TCAACTCTAA CTTTAACTTT GCACTAGTTG GACACCTCTT AAAAGGGTAC
    AGGCATCCTT CACCTGCCAT TGTTGCAAGA ACAGTCAGAA TTTTGCATAC ACTACTAACT
    CTCGTTAACA AACATAGAAA TTGTGACAAA TTTGAGGTGA ATACACAGAG TGTGGCTTAC
    TTAGCAGCTC TACTCACAGT GTCTGAAGAG GTTCGAAGTC GCTGTAGCCT CAAGCATAGG
    AAGTCCCTTC TTCTTACTGA TATTTCAATG GAAAATGTTC CTATGGATAC ATATCCCATT
    CATCACGGTG ACCCCAGCTA TAGGACACTA AAGGAGACCC AGCCATGGTC CTCTCCCAAA
    GGTTCAGAAG GATACCTTGC AGCCACCTAC CCAGCTGTAG GCCAAACCAG TCCCCGAGCC
    AGGAAATCCA TGAGCTTGGA TATGGGACAG CCTTCTCAGG CCAACACAAA AAAATTGCTT
    GGAACAAGAA AAAGTTTTGA TCACTTGATA TCTGACACAA AGGCTCCTAA AAGACAAGAA
    ATGGAATCAG GGATTACAAC ACCCCCGAAA ATGAGAAGAG TGGCAGAGAC TGACTATGAA
    ATGGCAGAAA CCCAAAGGAT TCCCTCCTCG CAGCAGCACC CACATTTACG GAAAGTCTCA
    GTCTCTGAAT CAAATGTTCT TTTGGATGAA GAAGTTCTTA CCGACCCAAA GATCCAAGCG
    CTGCTTCTTA CTGTTCTGGC TACACTGGTA AAATATACTA CAGATGAATT TGATCAACGG
    ATTCTTTATG AATATCTAGC AGAGGCCAGC GTTGTGTTTC CCAAAGTGTT TCCTGTTGTG
    CACAATTTGT TGGACTCTAA GATCAATACC CTCTTGTCTT TATGCCAAGA TCCAAATTTG
    TTAAATCCAA TCCATGGAAT TGTACAGAGT GTGGTGTACC ATGAGGAGTC CCCGCCACAG
    TACCAGACAT CTTACCTGCA AAGTTTTGGT TTTAATGGCT TATGGCGGTT TGCAGGACCC
    TTTTCAAAGC AAACACAAAT CCCTGACTAT GCTGAGCTCA TTGTTAAATT TCTTGATGCC
    TTGATTGACA CGTACTTGCC TGGAATTGAT GAAGAAACCA GTGAGGAGTC CCTCCTGACA
    CCCACATCTC CTTACCCTCC TGCACTGCAG AGCCAGCTTA GTATCACTGC CAACCTTAAC
    CTCTCTAATT CCATGACCTC ACTTGCAACT TCCCAGCATT CCCCAGGACT CGACAAGGAG
    AACGTTGAAC TCTCCCCCAC CGCTGGCCAC TGTAACAGTG GACGGACTCG CCATGGATCC
    GCAAGCCAAG TGCAGAAGCA AAGAAGTGCT GGCAGTTTCA AACGTAATAG CATTAAGAAG
    ATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532502.1
    CDS1612..10140
    Translation

    Target ORF information:

    RefSeq Version XM_006532439.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromatosis 1 (Nf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532439.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGC
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGCT GTGGAGCGCA CCCAGCAATA
    CGAATGGCAC CAAGTCTCAC CTTTAAAGAA AAGGTAACAA GCCTTAAATT TAAAGAAAAG
    CCTACAGACC TAGAGACAAG GAGCTACAAA TGTCTTCTTT TGTCCATGGT GAAACTGATT
    CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAGAGC
    AGTACAGCAG AACTAATTAC AGGGCTCGTA CAACTGGTTC CTCAGTCACA CATGCCAGAG
    GTCGCTCAGG AGGCTATGGA GGCTTTGCTG GTCCTTCATC AGTTAGATAG CATTGATCTC
    TGGAATCCTG ACGCTCCTGT AGAGACATTC TGGGAAATCA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA
    CGGGAAATTC TGATCTGCAG AAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGC
    TCCTGTCATT CTCTCTACCT TTATGGAGTA GGATGTGAAA TGTCTGCTAC TGGAAATACC
    ACTCAGATGT CAGTTGATCA TGATGAGTTC CTGCGTGCCT GTACTCCTGG AGCATCTCTG
    CGGAAAGGAA GAGGGAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCT
    ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCCTTAT ACATGTTTCT GTGGAATCCT
    GACACTGAAG CTGTTCTGGT TGCTATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT
    ATTCGATGTG GGGTAGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CCTCAGTCAG CAATATGATG TCAACAGGAA GAGCAGCGCT TCAGAAAAGA
    GTGATGGCCC TGCTAAGGCG CATTGAGCAC CCTACTGCAG GAAACATTGA GGCCTGGGAA
    GATACACATG CAAAGTGGGA ACAGGCTACA AAACTAATCC TTAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC AGAAAGTCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC
    CATGTCAGTG GAGGAGGCTC CATAGACTTG TCTGACACAG ACTCCCTGCA GGAGTGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT
    GGCCTAGCGA CATACAGCCC ACCTATGGGC GCTGTCAGTG AACGCAAAGG GTCTATGATT
    TCTGTAATGT CTTCTGAAGG GAATATTGAT TCACCTGTCA GCAGATTTAT GGACCGGCTT
    CTGTCCTTAA TGGTGTGTAA CCACGAGAAA GTGGGGCTTC AGATACGGAC CAATGTTAAG
    GACCTGGTGG GTCTGGAGTT GAGTCCTGCT CTGTATCCAA TGCTGTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TACTAAGTGA CAGCAATACT
    CAGTTTGTAG AGCAAACCAT AGCCATAATG AAAAACCTAC TGGATAATCA TACTGAAGGC
    AGCTCTGAGC ATCTGGGACA AGCGAGCATT GAGACAATGA TGCTAAATCT GGTCAGATAC
    GTTCGTGTTC TTGGGAATAT GGTCCATGCA ATACAAATAA AAACAAAATT GTGTCAGTTA
    GTTGAAGTCA TGATGGCAAG AAGAGATGAC CTCTCGTTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT AACAGACTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGACATAA AATGTCTTAC GAGAGATCTG GACCAAGCAA GCATGGAAGC AGTAGTTTCT
    CTCCTCGCTG GTCTTCCACT GCAGCCCGAA GAAGGAGATG GGGTGGAGCT GATGGAGGCC
    AAATCACAGT TATTCCTTAA GTACTTCACA TTATTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACGGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCCCTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC
    AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCACAAGG ATCTTCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTCGATAC ACTTGCGGAA
    ACGGTGTTGG CAGATCGATT TGAGAGACTT GTGGAACTGG TCACAATGAT GGGAGACCAG
    GGAGAACTCC CTATAGCTAT GGCTCTTGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG
    CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT TCCCGGCATT TACTCTACCA GCTGCTCTGG
    AACATGTTTT CTAAGGAGGT AGAATTGGCA GACTCCATGC AGACTCTCTT TCGAGGCAAC
    AGCTTGGCCA GTAAGATAAT GACATTCTGC TTCAAGGTGT ATGGTGCTAC TTACCTACAA
    AAGCTCTTGG ACCCTTTATT ACGAGTCATC ATCACATCTT CGGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACCC TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC
    CTCCTTCAGA TGACAGAGAA GTTCTTCCAT GCCATCATCA GCTCTTCCTC AGAATTCCCC
    TCACAGCTTC GAAGTGTCTG CCATTGTTTG TACCAGGCAA CTTGCCACTC CCTACTGAAT
    AAAGCTACAG TAAAAGAAAG AAAGGAAAAC AAAAAATCAG TGGTTAGCCA GCGCTTCCCT
    CAGAACAGCA TCGGTGCCGT AGGAAGTGCC ATGTTTCTCA GATTCATCAA TCCTGCCATT
    GTCTCGCCGT ATGAAGCAGG GATTTTAGAT AAAAAGCCAC CACCTAGAAT TGAAAGGGGC
    TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC ATTGCCAATC ATGTACTGTT CACAAAAGAA
    GAGCATATGA GACCTTTTAA TGATTTTGTG AAAAGCAACT TTGACTTGGC ACGAAGGTTT
    TTCCTAGATA TAGCATCAGA TTGTCCCACA AGTGATGCAG TAAACCATAG TCTTTCCTTC
    ATCAGCGATG GCAATGTGCT TGCTTTACAT CGGCTGCTTT GGAACAATCA GGAGAAAATT
    GGCCAGTATC TTTCCAGTAA CAGGGATCAT AAAGCTGTTG GAAGACGACC TTTTGATAAG
    ATGGCCACAC TTCTCGCGTA TCTGGGTCCT CCGGAGCACA AGCCTGTGGC AGATACACAC
    TGGTCCAGCC TTAACCTTAC CAGTTCAAAG TTTGAGGAAT TTATGACCAG GCACCAGGTA
    CATGAGAAAG AAGAGTTCAA GGCCTTGAAA ACGTTAAGCA TCTTCTACCA AGCTGGCACC
    TCCAAAGCTG GGAATCCTAT TTTTTATTAT GTTGCACGGA GGTTCAAAAC TGGCCAGATC
    AATGGTGATC TGCTGATATA CCATGTCTTG CTGACTTTAA AGCCATATTA TGCAAAGCCA
    TATGAAATTG TAGTGGACCT TACCCATACT GGGCCTAGCA ATCGCTTTAA AACAGACTTC
    CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC TTTGCCTATG ACAACGTCTC TGCAGTCTAT
    ATCTATAACT GTAACTCCTG GGTCAGGGAG TATACCAAAT ACCATGAGCG GCTGCTGACT
    GGCCTCAAGG GCAGCAAAAG GCTCATTTTC ATCGACTGTC CTGGGAAGCT GGCTGAACAT
    ATAGAGCATG AGCAACAGAA ACTCCCTGCT GCTACCTTGG CTTTGGAAGA AGATCTAAAG
    GTTTTCCACA ATGCTCTCAA GTTAGCTCAC AAGGACACCA AAGTTTCTAT TAAGGTTGGT
    TCTACTGCTG TTCAGGTAAC TTCAGCAGAA AGAACAAAAG TTTTGGGGCA ATCCGTCTTT
    TTAAATGATA TTTACTATGC TTCTGAAATC GAAGAGATCT GCCTAGTTGA TGAGAACCAG
    TTCACTCTAA CCATTGCAAA CCAAGGCACA CCACTGACCT TCATGCACCA AGAATGTGAA
    GCCATTGTTC AGTCTATCAT TCATATCAGG ACCCGCTGGG AGCTGTCACA ACCTGACTCC
    ATCCCTCAGC ATACCAAGAT TCGACCAAAG GATGTCCCTG GGACACTGCT TAATATTGCG
    TTACTGAATT TGGGCAGTTC AGACCCTAGT TTACGGTCTG CTGCCTATAA TCTCCTGTGT
    GCCTTAACCT GCACCTTTAA TTTAAAGATT GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA
    TGCATCCCTG CCAACAACAC ACTCTTCATT GTCTCTATCA GTAAGACGCT CGCAGCCAAT
    GAGCCACACC TCACCTTAGA GTTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAGT
    ATTGAATTGA AGCACCTTTG TTTGGAATAT ATGACCCCAT GGCTGTCAAA TCTAGTCCGT
    TTTTGTAAGC ATAATGATGA TGCCAAACGA CAAAGGGTTA CTGCCATCCT TGATAAGCTA
    ATAACAATGA CTATAAACGA GAAGCAGATG TATCCTTCTA TTCAAGCAAA AATCTGGGGG
    AGCCTTGGGC AGATCACAGA CCTGCTGGAT GTTGTGCTTG ACAGTTTCAT CAAGACCAGT
    GCGACAGGAG GCTTAGGGTC TATCAAAGCT GAGGTGATGG CAGACACAGC TGTGGCTTTA
    GCTTCTGGAA ATGTGAAATT GGTGTCGAGT AAGGTTATTG GAAGGATGTG TAAAATAATT
    GACAAGACTT GCTTATCCCC AACTCCAACT TTAGAACAAC ATCTTATGTG GGACGACATT
    GCCATTTTAG CCCGCTACAT GCTGATGCTG TCCTTCAACA ACTCCCTCGA TGTGGCGGCT
    CATCTGCCCT ATCTCTTCCA TGTTGTCACT TTCTTAGTAG CCACAGGTCC CTTGTCCCTC
    CGAGCTTCCA CACATGGGCT GCTCATCAAT ATCATTCACT CTCTGTGTAC TTGTTCTCAG
    CTTCACTTTA GTGAAGAGAC CAAGCAAGTT TTGAGGCTCA GTCTAACAGA GTTCTCGTTA
    CCCAAATTTT ACTTACTGTT TGGCATTAGC AAAGTCAAGT CGGCTGCTGT CATTGCCTTC
    CGTTCCAGTT ACCGGGACCG CTCCTTCTCC CCTGGCTCCT ATGAGAGGGA GACTTTTGCT
    TTGACGTCCC TGGAAACAGT CACAGAAGCT TTGTTGGAGA TCATGGAGGC ATGTATGAGA
    GATATTCCAA CATGCAAGTG GCTGGATCAG TGGACAGAAC TAGCTCAAAG ATTTGCGTTT
    CAGTATAACC CATCGCTGCA GCCAAGAGCT CTTGTGGTGT TTGGCTGTAT TAGCAAACGA
    GTGTCTCATG GGCAGATAAA GCAGATTATC CGAATTCTTA GCAAGGCACT TGAAAGTTGT
    TTAAAAGGAC CTGACACTTA CAACAGTCAA GTTCTGATAG AATCTACGGT GATAGCACTA
    ACAAAATTAC AGCCGCTTCT TAATAAGGAC TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT
    GCTGTGGCTG TGCTGCAGCT GGACGAAGTC AACTTGTATT CAGCCGGCAC TGCACTTCTG
    GAACAAAACC TGCACACCTT GGACAGTCTC CGGATATTCA ATGACAAGAG TCCGGAAGAA
    GTATTTATGG CGATCCGGAA TCCTCTGGAA TGGCACTGCA AGCAGATGGA TCACTTTGTC
    GGACTCAACT TCAACTCTAA CTTTAACTTT GCACTAGTTG GACACCTCTT AAAAGGGTAC
    AGGCATCCTT CACCTGCCAT TGTTGCAAGA ACAGTCAGAA TTTTGCATAC ACTACTAACT
    CTCGTTAACA AACATAGAAA TTGTGACAAA TTTGAGGTGA ATACACAGAG TGTGGCTTAC
    TTAGCAGCTC TACTCACAGT GTCTGAAGAG GTTCGAAGTC GCTGTAGCCT CAAGCATAGG
    AAGTCCCTTC TTCTTACTGA TATTTCAATG GAAAATGTTC CTATGGATAC ATATCCCATT
    CATCACGGTG ACCCCAGCTA TAGGACACTA AAGGAGACCC AGCCATGGTC CTCTCCCAAA
    GGTTCAGAAG GATACCTTGC AGCCACCTAC CCAGCTGTAG GCCAAACCAG TCCCCGAGCC
    AGGAAATCCA TGAGCTTGGA TATGGGACAG CCTTCTCAGG CCAACACAAA AAAATTGCTT
    GGAACAAGAA AAAGTTTTGA TCACTTGATA TCTGACACAA AGGCTCCTAA AAGACAAGAA
    ATGGAATCAG GGATTACAAC ACCCCCGAAA ATGAGAAGAG TGGCAGAGAC TGACTATGAA
    ATGGCAGAAA CCCAAAGGAT TCCCTCCTCG CAGCAGCACC CACATTTACG GAAAGTCTCA
    GTCTCTGAAT CAAATGTTCT TTTGGATGAA GAAGTTCTTA CCGACCCAAA GATCCAAGCG
    CTGCTTCTTA CTGTTCTGGC TACACTGGTA AAATATACTA CAGATGAATT TGATCAACGG
    ATTCTTTATG AATATCTAGC AGAGGCCAGC GTTGTGTTTC CCAAAGTGTT TCCTGTTGTG
    CACAATTTGT TGGACTCTAA GATCAATACC CTCTTGTCTT TATGCCAAGA TCCAAATTTG
    TTAAATCCAA TCCATGGAAT TGTACAGAGT GTGGTGTACC ATGAGGAGTC CCCGCCACAG
    TACCAGACAT CTTACCTGCA AAGTTTTGGT TTTAATGGCT TATGGCGGTT TGCAGGACCC
    TTTTCAAAGC AAACACAAAT CCCTGACTAT GCTGAGCTCA TTGTTAAATT TCTTGATGCC
    TTGATTGACA CGTACTTGCC TGGAATTGAT GAAGAAACCA GTGAGGAGTC CCTCCTGACA
    CCCACATCTC CTTACCCTCC TGCACTGCAG AGCCAGCTTA GTATCACTGC CAACCTTAAC
    CTCTCTAATT CCATGACCTC ACTTGCAACT TCCCAGCATT CCCCAGGACT CGACAAGGAG
    AACGTTGAAC TCTCCCCCAC CGCTGGCCAC TGTAACAGTG GACGGACTCG CCATGGATCC
    GCAAGCCAAG TGCAGAAGCA AAGAAGTGCT GGCAGTTTCA AACGTAATAG CATTAAGAAG
    ATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45705
    Clone ID Related Accession (Same CDS sequence) XM_006532439.3 , XM_006532439.4
    Accession Version XM_006532439.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8529bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532439.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGC
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGCT GTGGAGCGCA CCCAGCAATA
    CGAATGGCAC CAAGTCTCAC CTTTAAAGAA AAGGTAACAA GCCTTAAATT TAAAGAAAAG
    CCTACAGACC TAGAGACAAG GAGCTACAAA TGTCTTCTTT TGTCCATGGT GAAACTGATT
    CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAGAGC
    AGTACAGCAG AACTAATTAC AGGGCTCGTA CAACTGGTTC CTCAGTCACA CATGCCAGAG
    GTCGCTCAGG AGGCTATGGA GGCTTTGCTG GTCCTTCATC AGTTAGATAG CATTGATCTC
    TGGAATCCTG ACGCTCCTGT AGAGACATTC TGGGAAATCA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA
    CGGGAAATTC TGATCTGCAG AAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGC
    TCCTGTCATT CTCTCTACCT TTATGGAGTA GGATGTGAAA TGTCTGCTAC TGGAAATACC
    ACTCAGATGT CAGTTGATCA TGATGAGTTC CTGCGTGCCT GTACTCCTGG AGCATCTCTG
    CGGAAAGGAA GAGGGAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCT
    ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCCTTAT ACATGTTTCT GTGGAATCCT
    GACACTGAAG CTGTTCTGGT TGCTATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT
    ATTCGATGTG GGGTAGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CCTCAGTCAG CAATATGATG TCAACAGGAA GAGCAGCGCT TCAGAAAAGA
    GTGATGGCCC TGCTAAGGCG CATTGAGCAC CCTACTGCAG GAAACATTGA GGCCTGGGAA
    GATACACATG CAAAGTGGGA ACAGGCTACA AAACTAATCC TTAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC AGAAAGTCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC
    CATGTCAGTG GAGGAGGCTC CATAGACTTG TCTGACACAG ACTCCCTGCA GGAGTGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT
    GGCCTAGCGA CATACAGCCC ACCTATGGGC GCTGTCAGTG AACGCAAAGG GTCTATGATT
    TCTGTAATGT CTTCTGAAGG GAATATTGAT TCACCTGTCA GCAGATTTAT GGACCGGCTT
    CTGTCCTTAA TGGTGTGTAA CCACGAGAAA GTGGGGCTTC AGATACGGAC CAATGTTAAG
    GACCTGGTGG GTCTGGAGTT GAGTCCTGCT CTGTATCCAA TGCTGTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TACTAAGTGA CAGCAATACT
    CAGTTTGTAG AGCAAACCAT AGCCATAATG AAAAACCTAC TGGATAATCA TACTGAAGGC
    AGCTCTGAGC ATCTGGGACA AGCGAGCATT GAGACAATGA TGCTAAATCT GGTCAGATAC
    GTTCGTGTTC TTGGGAATAT GGTCCATGCA ATACAAATAA AAACAAAATT GTGTCAGTTA
    GTTGAAGTCA TGATGGCAAG AAGAGATGAC CTCTCGTTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT AACAGACTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGACATAA AATGTCTTAC GAGAGATCTG GACCAAGCAA GCATGGAAGC AGTAGTTTCT
    CTCCTCGCTG GTCTTCCACT GCAGCCCGAA GAAGGAGATG GGGTGGAGCT GATGGAGGCC
    AAATCACAGT TATTCCTTAA GTACTTCACA TTATTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACGGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCCCTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC
    AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCACAAGG ATCTTCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTCGATAC ACTTGCGGAA
    ACGGTGTTGG CAGATCGATT TGAGAGACTT GTGGAACTGG TCACAATGAT GGGAGACCAG
    GGAGAACTCC CTATAGCTAT GGCTCTTGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG
    CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT TCCCGGCATT TACTCTACCA GCTGCTCTGG
    AACATGTTTT CTAAGGAGGT AGAATTGGCA GACTCCATGC AGACTCTCTT TCGAGGCAAC
    AGCTTGGCCA GTAAGATAAT GACATTCTGC TTCAAGGTGT ATGGTGCTAC TTACCTACAA
    AAGCTCTTGG ACCCTTTATT ACGAGTCATC ATCACATCTT CGGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACCC TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC
    CTCCTTCAGA TGACAGAGAA GTTCTTCCAT GCCATCATCA GCTCTTCCTC AGAATTCCCC
    TCACAGCTTC GAAGTGTCTG CCATTGTTTG TACCAGGCAA CTTGCCACTC CCTACTGAAT
    AAAGCTACAG TAAAAGAAAG AAAGGAAAAC AAAAAATCAG TGGTTAGCCA GCGCTTCCCT
    CAGAACAGCA TCGGTGCCGT AGGAAGTGCC ATGTTTCTCA GATTCATCAA TCCTGCCATT
    GTCTCGCCGT ATGAAGCAGG GATTTTAGAT AAAAAGCCAC CACCTAGAAT TGAAAGGGGC
    TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC ATTGCCAATC ATGTACTGTT CACAAAAGAA
    GAGCATATGA GACCTTTTAA TGATTTTGTG AAAAGCAACT TTGACTTGGC ACGAAGGTTT
    TTCCTAGATA TAGCATCAGA TTGTCCCACA AGTGATGCAG TAAACCATAG TCTTTCCTTC
    ATCAGCGATG GCAATGTGCT TGCTTTACAT CGGCTGCTTT GGAACAATCA GGAGAAAATT
    GGCCAGTATC TTTCCAGTAA CAGGGATCAT AAAGCTGTTG GAAGACGACC TTTTGATAAG
    ATGGCCACAC TTCTCGCGTA TCTGGGTCCT CCGGAGCACA AGCCTGTGGC AGATACACAC
    TGGTCCAGCC TTAACCTTAC CAGTTCAAAG TTTGAGGAAT TTATGACCAG GCACCAGGTA
    CATGAGAAAG AAGAGTTCAA GGCCTTGAAA ACGTTAAGCA TCTTCTACCA AGCTGGCACC
    TCCAAAGCTG GGAATCCTAT TTTTTATTAT GTTGCACGGA GGTTCAAAAC TGGCCAGATC
    AATGGTGATC TGCTGATATA CCATGTCTTG CTGACTTTAA AGCCATATTA TGCAAAGCCA
    TATGAAATTG TAGTGGACCT TACCCATACT GGGCCTAGCA ATCGCTTTAA AACAGACTTC
    CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC TTTGCCTATG ACAACGTCTC TGCAGTCTAT
    ATCTATAACT GTAACTCCTG GGTCAGGGAG TATACCAAAT ACCATGAGCG GCTGCTGACT
    GGCCTCAAGG GCAGCAAAAG GCTCATTTTC ATCGACTGTC CTGGGAAGCT GGCTGAACAT
    ATAGAGCATG AGCAACAGAA ACTCCCTGCT GCTACCTTGG CTTTGGAAGA AGATCTAAAG
    GTTTTCCACA ATGCTCTCAA GTTAGCTCAC AAGGACACCA AAGTTTCTAT TAAGGTTGGT
    TCTACTGCTG TTCAGGTAAC TTCAGCAGAA AGAACAAAAG TTTTGGGGCA ATCCGTCTTT
    TTAAATGATA TTTACTATGC TTCTGAAATC GAAGAGATCT GCCTAGTTGA TGAGAACCAG
    TTCACTCTAA CCATTGCAAA CCAAGGCACA CCACTGACCT TCATGCACCA AGAATGTGAA
    GCCATTGTTC AGTCTATCAT TCATATCAGG ACCCGCTGGG AGCTGTCACA ACCTGACTCC
    ATCCCTCAGC ATACCAAGAT TCGACCAAAG GATGTCCCTG GGACACTGCT TAATATTGCG
    TTACTGAATT TGGGCAGTTC AGACCCTAGT TTACGGTCTG CTGCCTATAA TCTCCTGTGT
    GCCTTAACCT GCACCTTTAA TTTAAAGATT GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA
    TGCATCCCTG CCAACAACAC ACTCTTCATT GTCTCTATCA GTAAGACGCT CGCAGCCAAT
    GAGCCACACC TCACCTTAGA GTTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAGT
    ATTGAATTGA AGCACCTTTG TTTGGAATAT ATGACCCCAT GGCTGTCAAA TCTAGTCCGT
    TTTTGTAAGC ATAATGATGA TGCCAAACGA CAAAGGGTTA CTGCCATCCT TGATAAGCTA
    ATAACAATGA CTATAAACGA GAAGCAGATG TATCCTTCTA TTCAAGCAAA AATCTGGGGG
    AGCCTTGGGC AGATCACAGA CCTGCTGGAT GTTGTGCTTG ACAGTTTCAT CAAGACCAGT
    GCGACAGGAG GCTTAGGGTC TATCAAAGCT GAGGTGATGG CAGACACAGC TGTGGCTTTA
    GCTTCTGGAA ATGTGAAATT GGTGTCGAGT AAGGTTATTG GAAGGATGTG TAAAATAATT
    GACAAGACTT GCTTATCCCC AACTCCAACT TTAGAACAAC ATCTTATGTG GGACGACATT
    GCCATTTTAG CCCGCTACAT GCTGATGCTG TCCTTCAACA ACTCCCTCGA TGTGGCGGCT
    CATCTGCCCT ATCTCTTCCA TGTTGTCACT TTCTTAGTAG CCACAGGTCC CTTGTCCCTC
    CGAGCTTCCA CACATGGGCT GCTCATCAAT ATCATTCACT CTCTGTGTAC TTGTTCTCAG
    CTTCACTTTA GTGAAGAGAC CAAGCAAGTT TTGAGGCTCA GTCTAACAGA GTTCTCGTTA
    CCCAAATTTT ACTTACTGTT TGGCATTAGC AAAGTCAAGT CGGCTGCTGT CATTGCCTTC
    CGTTCCAGTT ACCGGGACCG CTCCTTCTCC CCTGGCTCCT ATGAGAGGGA GACTTTTGCT
    TTGACGTCCC TGGAAACAGT CACAGAAGCT TTGTTGGAGA TCATGGAGGC ATGTATGAGA
    GATATTCCAA CATGCAAGTG GCTGGATCAG TGGACAGAAC TAGCTCAAAG ATTTGCGTTT
    CAGTATAACC CATCGCTGCA GCCAAGAGCT CTTGTGGTGT TTGGCTGTAT TAGCAAACGA
    GTGTCTCATG GGCAGATAAA GCAGATTATC CGAATTCTTA GCAAGGCACT TGAAAGTTGT
    TTAAAAGGAC CTGACACTTA CAACAGTCAA GTTCTGATAG AATCTACGGT GATAGCACTA
    ACAAAATTAC AGCCGCTTCT TAATAAGGAC TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT
    GCTGTGGCTG TGCTGCAGCT GGACGAAGTC AACTTGTATT CAGCCGGCAC TGCACTTCTG
    GAACAAAACC TGCACACCTT GGACAGTCTC CGGATATTCA ATGACAAGAG TCCGGAAGAA
    GTATTTATGG CGATCCGGAA TCCTCTGGAA TGGCACTGCA AGCAGATGGA TCACTTTGTC
    GGACTCAACT TCAACTCTAA CTTTAACTTT GCACTAGTTG GACACCTCTT AAAAGGGTAC
    AGGCATCCTT CACCTGCCAT TGTTGCAAGA ACAGTCAGAA TTTTGCATAC ACTACTAACT
    CTCGTTAACA AACATAGAAA TTGTGACAAA TTTGAGGTGA ATACACAGAG TGTGGCTTAC
    TTAGCAGCTC TACTCACAGT GTCTGAAGAG GTTCGAAGTC GCTGTAGCCT CAAGCATAGG
    AAGTCCCTTC TTCTTACTGA TATTTCAATG GAAAATGTTC CTATGGATAC ATATCCCATT
    CATCACGGTG ACCCCAGCTA TAGGACACTA AAGGAGACCC AGCCATGGTC CTCTCCCAAA
    GGTTCAGAAG GATACCTTGC AGCCACCTAC CCAGCTGTAG GCCAAACCAG TCCCCGAGCC
    AGGAAATCCA TGAGCTTGGA TATGGGACAG CCTTCTCAGG CCAACACAAA AAAATTGCTT
    GGAACAAGAA AAAGTTTTGA TCACTTGATA TCTGACACAA AGGCTCCTAA AAGACAAGAA
    ATGGAATCAG GGATTACAAC ACCCCCGAAA ATGAGAAGAG TGGCAGAGAC TGACTATGAA
    ATGGCAGAAA CCCAAAGGAT TCCCTCCTCG CAGCAGCACC CACATTTACG GAAAGTCTCA
    GTCTCTGAAT CAAATGTTCT TTTGGATGAA GAAGTTCTTA CCGACCCAAA GATCCAAGCG
    CTGCTTCTTA CTGTTCTGGC TACACTGGTA AAATATACTA CAGATGAATT TGATCAACGG
    ATTCTTTATG AATATCTAGC AGAGGCCAGC GTTGTGTTTC CCAAAGTGTT TCCTGTTGTG
    CACAATTTGT TGGACTCTAA GATCAATACC CTCTTGTCTT TATGCCAAGA TCCAAATTTG
    TTAAATCCAA TCCATGGAAT TGTACAGAGT GTGGTGTACC ATGAGGAGTC CCCGCCACAG
    TACCAGACAT CTTACCTGCA AAGTTTTGGT TTTAATGGCT TATGGCGGTT TGCAGGACCC
    TTTTCAAAGC AAACACAAAT CCCTGACTAT GCTGAGCTCA TTGTTAAATT TCTTGATGCC
    TTGATTGACA CGTACTTGCC TGGAATTGAT GAAGAAACCA GTGAGGAGTC CCTCCTGACA
    CCCACATCTC CTTACCCTCC TGCACTGCAG AGCCAGCTTA GTATCACTGC CAACCTTAAC
    CTCTCTAATT CCATGACCTC ACTTGCAACT TCCCAGCATT CCCCAGGACT CGACAAGGAG
    AACGTTGAAC TCTCCCCCAC CGCTGGCCAC TGTAACAGTG GACGGACTCG CCATGGATCC
    GCAAGCCAAG TGCAGAAGCA AAGAAGTGCT GGCAGTTTCA AACGTAATAG CATTAAGAAG
    ATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532502.1
    CDS407..8935
    Translation

    Target ORF information:

    RefSeq Version XM_006532439.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromin 1 (Nf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532439.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGC
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGCT GTGGAGCGCA CCCAGCAATA
    CGAATGGCAC CAAGTCTCAC CTTTAAAGAA AAGGTAACAA GCCTTAAATT TAAAGAAAAG
    CCTACAGACC TAGAGACAAG GAGCTACAAA TGTCTTCTTT TGTCCATGGT GAAACTGATT
    CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAGAGC
    AGTACAGCAG AACTAATTAC AGGGCTCGTA CAACTGGTTC CTCAGTCACA CATGCCAGAG
    GTCGCTCAGG AGGCTATGGA GGCTTTGCTG GTCCTTCATC AGTTAGATAG CATTGATCTC
    TGGAATCCTG ACGCTCCTGT AGAGACATTC TGGGAAATCA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA
    CGGGAAATTC TGATCTGCAG AAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGC
    TCCTGTCATT CTCTCTACCT TTATGGAGTA GGATGTGAAA TGTCTGCTAC TGGAAATACC
    ACTCAGATGT CAGTTGATCA TGATGAGTTC CTGCGTGCCT GTACTCCTGG AGCATCTCTG
    CGGAAAGGAA GAGGGAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCT
    ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCCTTAT ACATGTTTCT GTGGAATCCT
    GACACTGAAG CTGTTCTGGT TGCTATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT
    ATTCGATGTG GGGTAGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CCTCAGTCAG CAATATGATG TCAACAGGAA GAGCAGCGCT TCAGAAAAGA
    GTGATGGCCC TGCTAAGGCG CATTGAGCAC CCTACTGCAG GAAACATTGA GGCCTGGGAA
    GATACACATG CAAAGTGGGA ACAGGCTACA AAACTAATCC TTAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC AGAAAGTCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC
    CATGTCAGTG GAGGAGGCTC CATAGACTTG TCTGACACAG ACTCCCTGCA GGAGTGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT
    GGCCTAGCGA CATACAGCCC ACCTATGGGC GCTGTCAGTG AACGCAAAGG GTCTATGATT
    TCTGTAATGT CTTCTGAAGG GAATATTGAT TCACCTGTCA GCAGATTTAT GGACCGGCTT
    CTGTCCTTAA TGGTGTGTAA CCACGAGAAA GTGGGGCTTC AGATACGGAC CAATGTTAAG
    GACCTGGTGG GTCTGGAGTT GAGTCCTGCT CTGTATCCAA TGCTGTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TACTAAGTGA CAGCAATACT
    CAGTTTGTAG AGCAAACCAT AGCCATAATG AAAAACCTAC TGGATAATCA TACTGAAGGC
    AGCTCTGAGC ATCTGGGACA AGCGAGCATT GAGACAATGA TGCTAAATCT GGTCAGATAC
    GTTCGTGTTC TTGGGAATAT GGTCCATGCA ATACAAATAA AAACAAAATT GTGTCAGTTA
    GTTGAAGTCA TGATGGCAAG AAGAGATGAC CTCTCGTTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT AACAGACTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGACATAA AATGTCTTAC GAGAGATCTG GACCAAGCAA GCATGGAAGC AGTAGTTTCT
    CTCCTCGCTG GTCTTCCACT GCAGCCCGAA GAAGGAGATG GGGTGGAGCT GATGGAGGCC
    AAATCACAGT TATTCCTTAA GTACTTCACA TTATTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACGGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCCCTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC
    AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCACAAGG ATCTTCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTCGATAC ACTTGCGGAA
    ACGGTGTTGG CAGATCGATT TGAGAGACTT GTGGAACTGG TCACAATGAT GGGAGACCAG
    GGAGAACTCC CTATAGCTAT GGCTCTTGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG
    CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT TCCCGGCATT TACTCTACCA GCTGCTCTGG
    AACATGTTTT CTAAGGAGGT AGAATTGGCA GACTCCATGC AGACTCTCTT TCGAGGCAAC
    AGCTTGGCCA GTAAGATAAT GACATTCTGC TTCAAGGTGT ATGGTGCTAC TTACCTACAA
    AAGCTCTTGG ACCCTTTATT ACGAGTCATC ATCACATCTT CGGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACCC TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC
    CTCCTTCAGA TGACAGAGAA GTTCTTCCAT GCCATCATCA GCTCTTCCTC AGAATTCCCC
    TCACAGCTTC GAAGTGTCTG CCATTGTTTG TACCAGGCAA CTTGCCACTC CCTACTGAAT
    AAAGCTACAG TAAAAGAAAG AAAGGAAAAC AAAAAATCAG TGGTTAGCCA GCGCTTCCCT
    CAGAACAGCA TCGGTGCCGT AGGAAGTGCC ATGTTTCTCA GATTCATCAA TCCTGCCATT
    GTCTCGCCGT ATGAAGCAGG GATTTTAGAT AAAAAGCCAC CACCTAGAAT TGAAAGGGGC
    TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC ATTGCCAATC ATGTACTGTT CACAAAAGAA
    GAGCATATGA GACCTTTTAA TGATTTTGTG AAAAGCAACT TTGACTTGGC ACGAAGGTTT
    TTCCTAGATA TAGCATCAGA TTGTCCCACA AGTGATGCAG TAAACCATAG TCTTTCCTTC
    ATCAGCGATG GCAATGTGCT TGCTTTACAT CGGCTGCTTT GGAACAATCA GGAGAAAATT
    GGCCAGTATC TTTCCAGTAA CAGGGATCAT AAAGCTGTTG GAAGACGACC TTTTGATAAG
    ATGGCCACAC TTCTCGCGTA TCTGGGTCCT CCGGAGCACA AGCCTGTGGC AGATACACAC
    TGGTCCAGCC TTAACCTTAC CAGTTCAAAG TTTGAGGAAT TTATGACCAG GCACCAGGTA
    CATGAGAAAG AAGAGTTCAA GGCCTTGAAA ACGTTAAGCA TCTTCTACCA AGCTGGCACC
    TCCAAAGCTG GGAATCCTAT TTTTTATTAT GTTGCACGGA GGTTCAAAAC TGGCCAGATC
    AATGGTGATC TGCTGATATA CCATGTCTTG CTGACTTTAA AGCCATATTA TGCAAAGCCA
    TATGAAATTG TAGTGGACCT TACCCATACT GGGCCTAGCA ATCGCTTTAA AACAGACTTC
    CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC TTTGCCTATG ACAACGTCTC TGCAGTCTAT
    ATCTATAACT GTAACTCCTG GGTCAGGGAG TATACCAAAT ACCATGAGCG GCTGCTGACT
    GGCCTCAAGG GCAGCAAAAG GCTCATTTTC ATCGACTGTC CTGGGAAGCT GGCTGAACAT
    ATAGAGCATG AGCAACAGAA ACTCCCTGCT GCTACCTTGG CTTTGGAAGA AGATCTAAAG
    GTTTTCCACA ATGCTCTCAA GTTAGCTCAC AAGGACACCA AAGTTTCTAT TAAGGTTGGT
    TCTACTGCTG TTCAGGTAAC TTCAGCAGAA AGAACAAAAG TTTTGGGGCA ATCCGTCTTT
    TTAAATGATA TTTACTATGC TTCTGAAATC GAAGAGATCT GCCTAGTTGA TGAGAACCAG
    TTCACTCTAA CCATTGCAAA CCAAGGCACA CCACTGACCT TCATGCACCA AGAATGTGAA
    GCCATTGTTC AGTCTATCAT TCATATCAGG ACCCGCTGGG AGCTGTCACA ACCTGACTCC
    ATCCCTCAGC ATACCAAGAT TCGACCAAAG GATGTCCCTG GGACACTGCT TAATATTGCG
    TTACTGAATT TGGGCAGTTC AGACCCTAGT TTACGGTCTG CTGCCTATAA TCTCCTGTGT
    GCCTTAACCT GCACCTTTAA TTTAAAGATT GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA
    TGCATCCCTG CCAACAACAC ACTCTTCATT GTCTCTATCA GTAAGACGCT CGCAGCCAAT
    GAGCCACACC TCACCTTAGA GTTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAGT
    ATTGAATTGA AGCACCTTTG TTTGGAATAT ATGACCCCAT GGCTGTCAAA TCTAGTCCGT
    TTTTGTAAGC ATAATGATGA TGCCAAACGA CAAAGGGTTA CTGCCATCCT TGATAAGCTA
    ATAACAATGA CTATAAACGA GAAGCAGATG TATCCTTCTA TTCAAGCAAA AATCTGGGGG
    AGCCTTGGGC AGATCACAGA CCTGCTGGAT GTTGTGCTTG ACAGTTTCAT CAAGACCAGT
    GCGACAGGAG GCTTAGGGTC TATCAAAGCT GAGGTGATGG CAGACACAGC TGTGGCTTTA
    GCTTCTGGAA ATGTGAAATT GGTGTCGAGT AAGGTTATTG GAAGGATGTG TAAAATAATT
    GACAAGACTT GCTTATCCCC AACTCCAACT TTAGAACAAC ATCTTATGTG GGACGACATT
    GCCATTTTAG CCCGCTACAT GCTGATGCTG TCCTTCAACA ACTCCCTCGA TGTGGCGGCT
    CATCTGCCCT ATCTCTTCCA TGTTGTCACT TTCTTAGTAG CCACAGGTCC CTTGTCCCTC
    CGAGCTTCCA CACATGGGCT GCTCATCAAT ATCATTCACT CTCTGTGTAC TTGTTCTCAG
    CTTCACTTTA GTGAAGAGAC CAAGCAAGTT TTGAGGCTCA GTCTAACAGA GTTCTCGTTA
    CCCAAATTTT ACTTACTGTT TGGCATTAGC AAAGTCAAGT CGGCTGCTGT CATTGCCTTC
    CGTTCCAGTT ACCGGGACCG CTCCTTCTCC CCTGGCTCCT ATGAGAGGGA GACTTTTGCT
    TTGACGTCCC TGGAAACAGT CACAGAAGCT TTGTTGGAGA TCATGGAGGC ATGTATGAGA
    GATATTCCAA CATGCAAGTG GCTGGATCAG TGGACAGAAC TAGCTCAAAG ATTTGCGTTT
    CAGTATAACC CATCGCTGCA GCCAAGAGCT CTTGTGGTGT TTGGCTGTAT TAGCAAACGA
    GTGTCTCATG GGCAGATAAA GCAGATTATC CGAATTCTTA GCAAGGCACT TGAAAGTTGT
    TTAAAAGGAC CTGACACTTA CAACAGTCAA GTTCTGATAG AATCTACGGT GATAGCACTA
    ACAAAATTAC AGCCGCTTCT TAATAAGGAC TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT
    GCTGTGGCTG TGCTGCAGCT GGACGAAGTC AACTTGTATT CAGCCGGCAC TGCACTTCTG
    GAACAAAACC TGCACACCTT GGACAGTCTC CGGATATTCA ATGACAAGAG TCCGGAAGAA
    GTATTTATGG CGATCCGGAA TCCTCTGGAA TGGCACTGCA AGCAGATGGA TCACTTTGTC
    GGACTCAACT TCAACTCTAA CTTTAACTTT GCACTAGTTG GACACCTCTT AAAAGGGTAC
    AGGCATCCTT CACCTGCCAT TGTTGCAAGA ACAGTCAGAA TTTTGCATAC ACTACTAACT
    CTCGTTAACA AACATAGAAA TTGTGACAAA TTTGAGGTGA ATACACAGAG TGTGGCTTAC
    TTAGCAGCTC TACTCACAGT GTCTGAAGAG GTTCGAAGTC GCTGTAGCCT CAAGCATAGG
    AAGTCCCTTC TTCTTACTGA TATTTCAATG GAAAATGTTC CTATGGATAC ATATCCCATT
    CATCACGGTG ACCCCAGCTA TAGGACACTA AAGGAGACCC AGCCATGGTC CTCTCCCAAA
    GGTTCAGAAG GATACCTTGC AGCCACCTAC CCAGCTGTAG GCCAAACCAG TCCCCGAGCC
    AGGAAATCCA TGAGCTTGGA TATGGGACAG CCTTCTCAGG CCAACACAAA AAAATTGCTT
    GGAACAAGAA AAAGTTTTGA TCACTTGATA TCTGACACAA AGGCTCCTAA AAGACAAGAA
    ATGGAATCAG GGATTACAAC ACCCCCGAAA ATGAGAAGAG TGGCAGAGAC TGACTATGAA
    ATGGCAGAAA CCCAAAGGAT TCCCTCCTCG CAGCAGCACC CACATTTACG GAAAGTCTCA
    GTCTCTGAAT CAAATGTTCT TTTGGATGAA GAAGTTCTTA CCGACCCAAA GATCCAAGCG
    CTGCTTCTTA CTGTTCTGGC TACACTGGTA AAATATACTA CAGATGAATT TGATCAACGG
    ATTCTTTATG AATATCTAGC AGAGGCCAGC GTTGTGTTTC CCAAAGTGTT TCCTGTTGTG
    CACAATTTGT TGGACTCTAA GATCAATACC CTCTTGTCTT TATGCCAAGA TCCAAATTTG
    TTAAATCCAA TCCATGGAAT TGTACAGAGT GTGGTGTACC ATGAGGAGTC CCCGCCACAG
    TACCAGACAT CTTACCTGCA AAGTTTTGGT TTTAATGGCT TATGGCGGTT TGCAGGACCC
    TTTTCAAAGC AAACACAAAT CCCTGACTAT GCTGAGCTCA TTGTTAAATT TCTTGATGCC
    TTGATTGACA CGTACTTGCC TGGAATTGAT GAAGAAACCA GTGAGGAGTC CCTCCTGACA
    CCCACATCTC CTTACCCTCC TGCACTGCAG AGCCAGCTTA GTATCACTGC CAACCTTAAC
    CTCTCTAATT CCATGACCTC ACTTGCAACT TCCCAGCATT CCCCAGGACT CGACAAGGAG
    AACGTTGAAC TCTCCCCCAC CGCTGGCCAC TGTAACAGTG GACGGACTCG CCATGGATCC
    GCAAGCCAAG TGCAGAAGCA AAGAAGTGCT GGCAGTTTCA AACGTAATAG CATTAAGAAG
    ATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45706
    Clone ID Related Accession (Same CDS sequence) XM_006532440.3 , XM_006532440.4
    Accession Version XM_006532440.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532440.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
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    2641
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    4141
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    6001
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    6301
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    6421
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    7141
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    7681
    7741
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    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGTGG TTAGCCAGCG CTTCCCTCAG AACAGCATCG GTGCCGTAGG AAGTGCCATG
    TTTCTCAGAT TCATCAATCC TGCCATTGTC TCGCCGTATG AAGCAGGGAT TTTAGATAAA
    AAGCCACCAC CTAGAATTGA AAGGGGCTTG AAGTTAATGT CAAAGGTACT TCAGAGCATT
    GCCAATCATG TACTGTTCAC AAAAGAAGAG CATATGAGAC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ACTTGGCACG AAGGTTTTTC CTAGATATAG CATCAGATTG TCCCACAAGT
    GATGCAGTAA ACCATAGTCT TTCCTTCATC AGCGATGGCA ATGTGCTTGC TTTACATCGG
    CTGCTTTGGA ACAATCAGGA GAAAATTGGC CAGTATCTTT CCAGTAACAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCCACACTTC TCGCGTATCT GGGTCCTCCG
    GAGCACAAGC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTT
    GAGGAATTTA TGACCAGGCA CCAGGTACAT GAGAAAGAAG AGTTCAAGGC CTTGAAAACG
    TTAAGCATCT TCTACCAAGC TGGCACCTCC AAAGCTGGGA ATCCTATTTT TTATTATGTT
    GCACGGAGGT TCAAAACTGG CCAGATCAAT GGTGATCTGC TGATATACCA TGTCTTGCTG
    ACTTTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACTGGG
    CCTAGCAATC GCTTTAAAAC AGACTTCCTC TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT
    GCCTATGACA ACGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAT
    ACCAAATACC ATGAGCGGCT GCTGACTGGC CTCAAGGGCA GCAAAAGGCT CATTTTCATC
    GACTGTCCTG GGAAGCTGGC TGAACATATA GAGCATGAGC AACAGAAACT CCCTGCTGCT
    ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT TTCCACAATG CTCTCAAGTT AGCTCACAAG
    GACACCAAAG TTTCTATTAA GGTTGGTTCT ACTGCTGTTC AGGTAACTTC AGCAGAAAGA
    ACAAAAGTTT TGGGGCAATC CGTCTTTTTA AATGATATTT ACTATGCTTC TGAAATCGAA
    GAGATCTGCC TAGTTGATGA GAACCAGTTC ACTCTAACCA TTGCAAACCA AGGCACACCA
    CTGACCTTCA TGCACCAAGA ATGTGAAGCC ATTGTTCAGT CTATCATTCA TATCAGGACC
    CGCTGGGAGC TGTCACAACC TGACTCCATC CCTCAGCATA CCAAGATTCG ACCAAAGGAT
    GTCCCTGGGA CACTGCTTAA TATTGCGTTA CTGAATTTGG GCAGTTCAGA CCCTAGTTTA
    CGGTCTGCTG CCTATAATCT CCTGTGTGCC TTAACCTGCA CCTTTAATTT AAAGATTGAA
    GGTCAGTTGC TAGAGACATC GGGGTTATGC ATCCCTGCCA ACAACACACT CTTCATTGTC
    TCTATCAGTA AGACGCTCGC AGCCAATGAG CCACACCTCA CCTTAGAGTT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAATTGAAGC ACCTTTGTTT GGAATATATG
    ACCCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCATA ATGATGATGC CAAACGACAA
    AGGGTTACTG CCATCCTTGA TAAGCTAATA ACAATGACTA TAAACGAGAA GCAGATGTAT
    CCTTCTATTC AAGCAAAAAT CTGGGGGAGC CTTGGGCAGA TCACAGACCT GCTGGATGTT
    GTGCTTGACA GTTTCATCAA GACCAGTGCG ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG
    GTGATGGCAG ACACAGCTGT GGCTTTAGCT TCTGGAAATG TGAAATTGGT GTCGAGTAAG
    GTTATTGGAA GGATGTGTAA AATAATTGAC AAGACTTGCT TATCCCCAAC TCCAACTTTA
    GAACAACATC TTATGTGGGA CGACATTGCC ATTTTAGCCC GCTACATGCT GATGCTGTCC
    TTCAACAACT CCCTCGATGT GGCGGCTCAT CTGCCCTATC TCTTCCATGT TGTCACTTTC
    TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA GCTTCCACAC ATGGGCTGCT CATCAATATC
    ATTCACTCTC TGTGTACTTG TTCTCAGCTT CACTTTAGTG AAGAGACCAA GCAAGTTTTG
    AGGCTCAGTC TAACAGAGTT CTCGTTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT TCCAGTTACC GGGACCGCTC CTTCTCCCCT
    GGCTCCTATG AGAGGGAGAC TTTTGCTTTG ACGTCCCTGG AAACAGTCAC AGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGATT TGCGTTTCAG TATAACCCAT CGCTGCAGCC AAGAGCTCTT
    GTGGTGTTTG GCTGTATTAG CAAACGAGTG TCTCATGGGC AGATAAAGCA GATTATCCGA
    ATTCTTAGCA AGGCACTTGA AAGTTGTTTA AAAGGACCTG ACACTTACAA CAGTCAAGTT
    CTGATAGAAT CTACGGTGAT AGCACTAACA AAATTACAGC CGCTTCTTAA TAAGGACTCG
    CCCCTGCACA AAGCCCTCTT TTGGGTTGCT GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC
    TTGTATTCAG CCGGCACTGC ACTTCTGGAA CAAAACCTGC ACACCTTGGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC GGAAGAAGTA TTTATGGCGA TCCGGAATCC TCTGGAATGG
    CACTGCAAGC AGATGGATCA CTTTGTCGGA CTCAACTTCA ACTCTAACTT TAACTTTGCA
    CTAGTTGGAC ACCTCTTAAA AGGGTACAGG CATCCTTCAC CTGCCATTGT TGCAAGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTC GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CACAGAGTGT GGCTTACTTA GCAGCTCTAC TCACAGTGTC TGAAGAGGTT
    CGAAGTCGCT GTAGCCTCAA GCATAGGAAG TCCCTTCTTC TTACTGATAT TTCAATGGAA
    AATGTTCCTA TGGATACATA TCCCATTCAT CACGGTGACC CCAGCTATAG GACACTAAAG
    GAGACCCAGC CATGGTCCTC TCCCAAAGGT TCAGAAGGAT ACCTTGCAGC CACCTACCCA
    GCTGTAGGCC AAACCAGTCC CCGAGCCAGG AAATCCATGA GCTTGGATAT GGGACAGCCT
    TCTCAGGCCA ACACAAAAAA ATTGCTTGGA ACAAGAAAAA GTTTTGATCA CTTGATATCT
    GACACAAAGG CTCCTAAAAG ACAAGAAATG GAATCAGGGA TTACAACACC CCCGAAAATG
    AGAAGAGTGG CAGAGACTGA CTATGAAATG GCAGAAACCC AAAGGATTCC CTCCTCGCAG
    CAGCACCCAC ATTTACGGAA AGTCTCAGTC TCTGAATCAA ATGTTCTTTT GGATGAAGAA
    GTTCTTACCG ACCCAAAGAT CCAAGCGCTG CTTCTTACTG TTCTGGCTAC ACTGGTAAAA
    TATACTACAG ATGAATTTGA TCAACGGATT CTTTATGAAT ATCTAGCAGA GGCCAGCGTT
    GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC AATTTGTTGG ACTCTAAGAT CAATACCCTC
    TTGTCTTTAT GCCAAGATCC AAATTTGTTA AATCCAATCC ATGGAATTGT ACAGAGTGTG
    GTGTACCATG AGGAGTCCCC GCCACAGTAC CAGACATCTT ACCTGCAAAG TTTTGGTTTT
    AATGGCTTAT GGCGGTTTGC AGGACCCTTT TCAAAGCAAA CACAAATCCC TGACTATGCT
    GAGCTCATTG TTAAATTTCT TGATGCCTTG ATTGACACGT ACTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ACCCTCCTGC ACTGCAGAGC
    CAGCTTAGTA TCACTGCCAA CCTTAACCTC TCTAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGACTCGA CAAGGAGAAC GTTGAACTCT CCCCCACCGC TGGCCACTGT
    AACAGTGGAC GGACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532503.1
    CDS1612..10107
    Translation

    Target ORF information:

    RefSeq Version XM_006532440.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromatosis 1 (Nf1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532440.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGTGG TTAGCCAGCG CTTCCCTCAG AACAGCATCG GTGCCGTAGG AAGTGCCATG
    TTTCTCAGAT TCATCAATCC TGCCATTGTC TCGCCGTATG AAGCAGGGAT TTTAGATAAA
    AAGCCACCAC CTAGAATTGA AAGGGGCTTG AAGTTAATGT CAAAGGTACT TCAGAGCATT
    GCCAATCATG TACTGTTCAC AAAAGAAGAG CATATGAGAC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ACTTGGCACG AAGGTTTTTC CTAGATATAG CATCAGATTG TCCCACAAGT
    GATGCAGTAA ACCATAGTCT TTCCTTCATC AGCGATGGCA ATGTGCTTGC TTTACATCGG
    CTGCTTTGGA ACAATCAGGA GAAAATTGGC CAGTATCTTT CCAGTAACAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCCACACTTC TCGCGTATCT GGGTCCTCCG
    GAGCACAAGC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTT
    GAGGAATTTA TGACCAGGCA CCAGGTACAT GAGAAAGAAG AGTTCAAGGC CTTGAAAACG
    TTAAGCATCT TCTACCAAGC TGGCACCTCC AAAGCTGGGA ATCCTATTTT TTATTATGTT
    GCACGGAGGT TCAAAACTGG CCAGATCAAT GGTGATCTGC TGATATACCA TGTCTTGCTG
    ACTTTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACTGGG
    CCTAGCAATC GCTTTAAAAC AGACTTCCTC TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT
    GCCTATGACA ACGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAT
    ACCAAATACC ATGAGCGGCT GCTGACTGGC CTCAAGGGCA GCAAAAGGCT CATTTTCATC
    GACTGTCCTG GGAAGCTGGC TGAACATATA GAGCATGAGC AACAGAAACT CCCTGCTGCT
    ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT TTCCACAATG CTCTCAAGTT AGCTCACAAG
    GACACCAAAG TTTCTATTAA GGTTGGTTCT ACTGCTGTTC AGGTAACTTC AGCAGAAAGA
    ACAAAAGTTT TGGGGCAATC CGTCTTTTTA AATGATATTT ACTATGCTTC TGAAATCGAA
    GAGATCTGCC TAGTTGATGA GAACCAGTTC ACTCTAACCA TTGCAAACCA AGGCACACCA
    CTGACCTTCA TGCACCAAGA ATGTGAAGCC ATTGTTCAGT CTATCATTCA TATCAGGACC
    CGCTGGGAGC TGTCACAACC TGACTCCATC CCTCAGCATA CCAAGATTCG ACCAAAGGAT
    GTCCCTGGGA CACTGCTTAA TATTGCGTTA CTGAATTTGG GCAGTTCAGA CCCTAGTTTA
    CGGTCTGCTG CCTATAATCT CCTGTGTGCC TTAACCTGCA CCTTTAATTT AAAGATTGAA
    GGTCAGTTGC TAGAGACATC GGGGTTATGC ATCCCTGCCA ACAACACACT CTTCATTGTC
    TCTATCAGTA AGACGCTCGC AGCCAATGAG CCACACCTCA CCTTAGAGTT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAATTGAAGC ACCTTTGTTT GGAATATATG
    ACCCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCATA ATGATGATGC CAAACGACAA
    AGGGTTACTG CCATCCTTGA TAAGCTAATA ACAATGACTA TAAACGAGAA GCAGATGTAT
    CCTTCTATTC AAGCAAAAAT CTGGGGGAGC CTTGGGCAGA TCACAGACCT GCTGGATGTT
    GTGCTTGACA GTTTCATCAA GACCAGTGCG ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG
    GTGATGGCAG ACACAGCTGT GGCTTTAGCT TCTGGAAATG TGAAATTGGT GTCGAGTAAG
    GTTATTGGAA GGATGTGTAA AATAATTGAC AAGACTTGCT TATCCCCAAC TCCAACTTTA
    GAACAACATC TTATGTGGGA CGACATTGCC ATTTTAGCCC GCTACATGCT GATGCTGTCC
    TTCAACAACT CCCTCGATGT GGCGGCTCAT CTGCCCTATC TCTTCCATGT TGTCACTTTC
    TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA GCTTCCACAC ATGGGCTGCT CATCAATATC
    ATTCACTCTC TGTGTACTTG TTCTCAGCTT CACTTTAGTG AAGAGACCAA GCAAGTTTTG
    AGGCTCAGTC TAACAGAGTT CTCGTTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT TCCAGTTACC GGGACCGCTC CTTCTCCCCT
    GGCTCCTATG AGAGGGAGAC TTTTGCTTTG ACGTCCCTGG AAACAGTCAC AGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGATT TGCGTTTCAG TATAACCCAT CGCTGCAGCC AAGAGCTCTT
    GTGGTGTTTG GCTGTATTAG CAAACGAGTG TCTCATGGGC AGATAAAGCA GATTATCCGA
    ATTCTTAGCA AGGCACTTGA AAGTTGTTTA AAAGGACCTG ACACTTACAA CAGTCAAGTT
    CTGATAGAAT CTACGGTGAT AGCACTAACA AAATTACAGC CGCTTCTTAA TAAGGACTCG
    CCCCTGCACA AAGCCCTCTT TTGGGTTGCT GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC
    TTGTATTCAG CCGGCACTGC ACTTCTGGAA CAAAACCTGC ACACCTTGGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC GGAAGAAGTA TTTATGGCGA TCCGGAATCC TCTGGAATGG
    CACTGCAAGC AGATGGATCA CTTTGTCGGA CTCAACTTCA ACTCTAACTT TAACTTTGCA
    CTAGTTGGAC ACCTCTTAAA AGGGTACAGG CATCCTTCAC CTGCCATTGT TGCAAGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTC GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CACAGAGTGT GGCTTACTTA GCAGCTCTAC TCACAGTGTC TGAAGAGGTT
    CGAAGTCGCT GTAGCCTCAA GCATAGGAAG TCCCTTCTTC TTACTGATAT TTCAATGGAA
    AATGTTCCTA TGGATACATA TCCCATTCAT CACGGTGACC CCAGCTATAG GACACTAAAG
    GAGACCCAGC CATGGTCCTC TCCCAAAGGT TCAGAAGGAT ACCTTGCAGC CACCTACCCA
    GCTGTAGGCC AAACCAGTCC CCGAGCCAGG AAATCCATGA GCTTGGATAT GGGACAGCCT
    TCTCAGGCCA ACACAAAAAA ATTGCTTGGA ACAAGAAAAA GTTTTGATCA CTTGATATCT
    GACACAAAGG CTCCTAAAAG ACAAGAAATG GAATCAGGGA TTACAACACC CCCGAAAATG
    AGAAGAGTGG CAGAGACTGA CTATGAAATG GCAGAAACCC AAAGGATTCC CTCCTCGCAG
    CAGCACCCAC ATTTACGGAA AGTCTCAGTC TCTGAATCAA ATGTTCTTTT GGATGAAGAA
    GTTCTTACCG ACCCAAAGAT CCAAGCGCTG CTTCTTACTG TTCTGGCTAC ACTGGTAAAA
    TATACTACAG ATGAATTTGA TCAACGGATT CTTTATGAAT ATCTAGCAGA GGCCAGCGTT
    GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC AATTTGTTGG ACTCTAAGAT CAATACCCTC
    TTGTCTTTAT GCCAAGATCC AAATTTGTTA AATCCAATCC ATGGAATTGT ACAGAGTGTG
    GTGTACCATG AGGAGTCCCC GCCACAGTAC CAGACATCTT ACCTGCAAAG TTTTGGTTTT
    AATGGCTTAT GGCGGTTTGC AGGACCCTTT TCAAAGCAAA CACAAATCCC TGACTATGCT
    GAGCTCATTG TTAAATTTCT TGATGCCTTG ATTGACACGT ACTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ACCCTCCTGC ACTGCAGAGC
    CAGCTTAGTA TCACTGCCAA CCTTAACCTC TCTAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGACTCGA CAAGGAGAAC GTTGAACTCT CCCCCACCGC TGGCCACTGT
    AACAGTGGAC GGACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45706
    Clone ID Related Accession (Same CDS sequence) XM_006532440.3 , XM_006532440.4
    Accession Version XM_006532440.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532440.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGTGG TTAGCCAGCG CTTCCCTCAG AACAGCATCG GTGCCGTAGG AAGTGCCATG
    TTTCTCAGAT TCATCAATCC TGCCATTGTC TCGCCGTATG AAGCAGGGAT TTTAGATAAA
    AAGCCACCAC CTAGAATTGA AAGGGGCTTG AAGTTAATGT CAAAGGTACT TCAGAGCATT
    GCCAATCATG TACTGTTCAC AAAAGAAGAG CATATGAGAC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ACTTGGCACG AAGGTTTTTC CTAGATATAG CATCAGATTG TCCCACAAGT
    GATGCAGTAA ACCATAGTCT TTCCTTCATC AGCGATGGCA ATGTGCTTGC TTTACATCGG
    CTGCTTTGGA ACAATCAGGA GAAAATTGGC CAGTATCTTT CCAGTAACAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCCACACTTC TCGCGTATCT GGGTCCTCCG
    GAGCACAAGC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTT
    GAGGAATTTA TGACCAGGCA CCAGGTACAT GAGAAAGAAG AGTTCAAGGC CTTGAAAACG
    TTAAGCATCT TCTACCAAGC TGGCACCTCC AAAGCTGGGA ATCCTATTTT TTATTATGTT
    GCACGGAGGT TCAAAACTGG CCAGATCAAT GGTGATCTGC TGATATACCA TGTCTTGCTG
    ACTTTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACTGGG
    CCTAGCAATC GCTTTAAAAC AGACTTCCTC TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT
    GCCTATGACA ACGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAT
    ACCAAATACC ATGAGCGGCT GCTGACTGGC CTCAAGGGCA GCAAAAGGCT CATTTTCATC
    GACTGTCCTG GGAAGCTGGC TGAACATATA GAGCATGAGC AACAGAAACT CCCTGCTGCT
    ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT TTCCACAATG CTCTCAAGTT AGCTCACAAG
    GACACCAAAG TTTCTATTAA GGTTGGTTCT ACTGCTGTTC AGGTAACTTC AGCAGAAAGA
    ACAAAAGTTT TGGGGCAATC CGTCTTTTTA AATGATATTT ACTATGCTTC TGAAATCGAA
    GAGATCTGCC TAGTTGATGA GAACCAGTTC ACTCTAACCA TTGCAAACCA AGGCACACCA
    CTGACCTTCA TGCACCAAGA ATGTGAAGCC ATTGTTCAGT CTATCATTCA TATCAGGACC
    CGCTGGGAGC TGTCACAACC TGACTCCATC CCTCAGCATA CCAAGATTCG ACCAAAGGAT
    GTCCCTGGGA CACTGCTTAA TATTGCGTTA CTGAATTTGG GCAGTTCAGA CCCTAGTTTA
    CGGTCTGCTG CCTATAATCT CCTGTGTGCC TTAACCTGCA CCTTTAATTT AAAGATTGAA
    GGTCAGTTGC TAGAGACATC GGGGTTATGC ATCCCTGCCA ACAACACACT CTTCATTGTC
    TCTATCAGTA AGACGCTCGC AGCCAATGAG CCACACCTCA CCTTAGAGTT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAATTGAAGC ACCTTTGTTT GGAATATATG
    ACCCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCATA ATGATGATGC CAAACGACAA
    AGGGTTACTG CCATCCTTGA TAAGCTAATA ACAATGACTA TAAACGAGAA GCAGATGTAT
    CCTTCTATTC AAGCAAAAAT CTGGGGGAGC CTTGGGCAGA TCACAGACCT GCTGGATGTT
    GTGCTTGACA GTTTCATCAA GACCAGTGCG ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG
    GTGATGGCAG ACACAGCTGT GGCTTTAGCT TCTGGAAATG TGAAATTGGT GTCGAGTAAG
    GTTATTGGAA GGATGTGTAA AATAATTGAC AAGACTTGCT TATCCCCAAC TCCAACTTTA
    GAACAACATC TTATGTGGGA CGACATTGCC ATTTTAGCCC GCTACATGCT GATGCTGTCC
    TTCAACAACT CCCTCGATGT GGCGGCTCAT CTGCCCTATC TCTTCCATGT TGTCACTTTC
    TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA GCTTCCACAC ATGGGCTGCT CATCAATATC
    ATTCACTCTC TGTGTACTTG TTCTCAGCTT CACTTTAGTG AAGAGACCAA GCAAGTTTTG
    AGGCTCAGTC TAACAGAGTT CTCGTTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT TCCAGTTACC GGGACCGCTC CTTCTCCCCT
    GGCTCCTATG AGAGGGAGAC TTTTGCTTTG ACGTCCCTGG AAACAGTCAC AGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGATT TGCGTTTCAG TATAACCCAT CGCTGCAGCC AAGAGCTCTT
    GTGGTGTTTG GCTGTATTAG CAAACGAGTG TCTCATGGGC AGATAAAGCA GATTATCCGA
    ATTCTTAGCA AGGCACTTGA AAGTTGTTTA AAAGGACCTG ACACTTACAA CAGTCAAGTT
    CTGATAGAAT CTACGGTGAT AGCACTAACA AAATTACAGC CGCTTCTTAA TAAGGACTCG
    CCCCTGCACA AAGCCCTCTT TTGGGTTGCT GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC
    TTGTATTCAG CCGGCACTGC ACTTCTGGAA CAAAACCTGC ACACCTTGGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC GGAAGAAGTA TTTATGGCGA TCCGGAATCC TCTGGAATGG
    CACTGCAAGC AGATGGATCA CTTTGTCGGA CTCAACTTCA ACTCTAACTT TAACTTTGCA
    CTAGTTGGAC ACCTCTTAAA AGGGTACAGG CATCCTTCAC CTGCCATTGT TGCAAGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTC GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CACAGAGTGT GGCTTACTTA GCAGCTCTAC TCACAGTGTC TGAAGAGGTT
    CGAAGTCGCT GTAGCCTCAA GCATAGGAAG TCCCTTCTTC TTACTGATAT TTCAATGGAA
    AATGTTCCTA TGGATACATA TCCCATTCAT CACGGTGACC CCAGCTATAG GACACTAAAG
    GAGACCCAGC CATGGTCCTC TCCCAAAGGT TCAGAAGGAT ACCTTGCAGC CACCTACCCA
    GCTGTAGGCC AAACCAGTCC CCGAGCCAGG AAATCCATGA GCTTGGATAT GGGACAGCCT
    TCTCAGGCCA ACACAAAAAA ATTGCTTGGA ACAAGAAAAA GTTTTGATCA CTTGATATCT
    GACACAAAGG CTCCTAAAAG ACAAGAAATG GAATCAGGGA TTACAACACC CCCGAAAATG
    AGAAGAGTGG CAGAGACTGA CTATGAAATG GCAGAAACCC AAAGGATTCC CTCCTCGCAG
    CAGCACCCAC ATTTACGGAA AGTCTCAGTC TCTGAATCAA ATGTTCTTTT GGATGAAGAA
    GTTCTTACCG ACCCAAAGAT CCAAGCGCTG CTTCTTACTG TTCTGGCTAC ACTGGTAAAA
    TATACTACAG ATGAATTTGA TCAACGGATT CTTTATGAAT ATCTAGCAGA GGCCAGCGTT
    GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC AATTTGTTGG ACTCTAAGAT CAATACCCTC
    TTGTCTTTAT GCCAAGATCC AAATTTGTTA AATCCAATCC ATGGAATTGT ACAGAGTGTG
    GTGTACCATG AGGAGTCCCC GCCACAGTAC CAGACATCTT ACCTGCAAAG TTTTGGTTTT
    AATGGCTTAT GGCGGTTTGC AGGACCCTTT TCAAAGCAAA CACAAATCCC TGACTATGCT
    GAGCTCATTG TTAAATTTCT TGATGCCTTG ATTGACACGT ACTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ACCCTCCTGC ACTGCAGAGC
    CAGCTTAGTA TCACTGCCAA CCTTAACCTC TCTAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGACTCGA CAAGGAGAAC GTTGAACTCT CCCCCACCGC TGGCCACTGT
    AACAGTGGAC GGACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532503.1
    CDS407..8902
    Translation

    Target ORF information:

    RefSeq Version XM_006532440.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromin 1 (Nf1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532440.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGTGG TTAGCCAGCG CTTCCCTCAG AACAGCATCG GTGCCGTAGG AAGTGCCATG
    TTTCTCAGAT TCATCAATCC TGCCATTGTC TCGCCGTATG AAGCAGGGAT TTTAGATAAA
    AAGCCACCAC CTAGAATTGA AAGGGGCTTG AAGTTAATGT CAAAGGTACT TCAGAGCATT
    GCCAATCATG TACTGTTCAC AAAAGAAGAG CATATGAGAC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ACTTGGCACG AAGGTTTTTC CTAGATATAG CATCAGATTG TCCCACAAGT
    GATGCAGTAA ACCATAGTCT TTCCTTCATC AGCGATGGCA ATGTGCTTGC TTTACATCGG
    CTGCTTTGGA ACAATCAGGA GAAAATTGGC CAGTATCTTT CCAGTAACAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCCACACTTC TCGCGTATCT GGGTCCTCCG
    GAGCACAAGC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTT
    GAGGAATTTA TGACCAGGCA CCAGGTACAT GAGAAAGAAG AGTTCAAGGC CTTGAAAACG
    TTAAGCATCT TCTACCAAGC TGGCACCTCC AAAGCTGGGA ATCCTATTTT TTATTATGTT
    GCACGGAGGT TCAAAACTGG CCAGATCAAT GGTGATCTGC TGATATACCA TGTCTTGCTG
    ACTTTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACTGGG
    CCTAGCAATC GCTTTAAAAC AGACTTCCTC TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT
    GCCTATGACA ACGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAT
    ACCAAATACC ATGAGCGGCT GCTGACTGGC CTCAAGGGCA GCAAAAGGCT CATTTTCATC
    GACTGTCCTG GGAAGCTGGC TGAACATATA GAGCATGAGC AACAGAAACT CCCTGCTGCT
    ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT TTCCACAATG CTCTCAAGTT AGCTCACAAG
    GACACCAAAG TTTCTATTAA GGTTGGTTCT ACTGCTGTTC AGGTAACTTC AGCAGAAAGA
    ACAAAAGTTT TGGGGCAATC CGTCTTTTTA AATGATATTT ACTATGCTTC TGAAATCGAA
    GAGATCTGCC TAGTTGATGA GAACCAGTTC ACTCTAACCA TTGCAAACCA AGGCACACCA
    CTGACCTTCA TGCACCAAGA ATGTGAAGCC ATTGTTCAGT CTATCATTCA TATCAGGACC
    CGCTGGGAGC TGTCACAACC TGACTCCATC CCTCAGCATA CCAAGATTCG ACCAAAGGAT
    GTCCCTGGGA CACTGCTTAA TATTGCGTTA CTGAATTTGG GCAGTTCAGA CCCTAGTTTA
    CGGTCTGCTG CCTATAATCT CCTGTGTGCC TTAACCTGCA CCTTTAATTT AAAGATTGAA
    GGTCAGTTGC TAGAGACATC GGGGTTATGC ATCCCTGCCA ACAACACACT CTTCATTGTC
    TCTATCAGTA AGACGCTCGC AGCCAATGAG CCACACCTCA CCTTAGAGTT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAATTGAAGC ACCTTTGTTT GGAATATATG
    ACCCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCATA ATGATGATGC CAAACGACAA
    AGGGTTACTG CCATCCTTGA TAAGCTAATA ACAATGACTA TAAACGAGAA GCAGATGTAT
    CCTTCTATTC AAGCAAAAAT CTGGGGGAGC CTTGGGCAGA TCACAGACCT GCTGGATGTT
    GTGCTTGACA GTTTCATCAA GACCAGTGCG ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG
    GTGATGGCAG ACACAGCTGT GGCTTTAGCT TCTGGAAATG TGAAATTGGT GTCGAGTAAG
    GTTATTGGAA GGATGTGTAA AATAATTGAC AAGACTTGCT TATCCCCAAC TCCAACTTTA
    GAACAACATC TTATGTGGGA CGACATTGCC ATTTTAGCCC GCTACATGCT GATGCTGTCC
    TTCAACAACT CCCTCGATGT GGCGGCTCAT CTGCCCTATC TCTTCCATGT TGTCACTTTC
    TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA GCTTCCACAC ATGGGCTGCT CATCAATATC
    ATTCACTCTC TGTGTACTTG TTCTCAGCTT CACTTTAGTG AAGAGACCAA GCAAGTTTTG
    AGGCTCAGTC TAACAGAGTT CTCGTTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT TCCAGTTACC GGGACCGCTC CTTCTCCCCT
    GGCTCCTATG AGAGGGAGAC TTTTGCTTTG ACGTCCCTGG AAACAGTCAC AGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGATT TGCGTTTCAG TATAACCCAT CGCTGCAGCC AAGAGCTCTT
    GTGGTGTTTG GCTGTATTAG CAAACGAGTG TCTCATGGGC AGATAAAGCA GATTATCCGA
    ATTCTTAGCA AGGCACTTGA AAGTTGTTTA AAAGGACCTG ACACTTACAA CAGTCAAGTT
    CTGATAGAAT CTACGGTGAT AGCACTAACA AAATTACAGC CGCTTCTTAA TAAGGACTCG
    CCCCTGCACA AAGCCCTCTT TTGGGTTGCT GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC
    TTGTATTCAG CCGGCACTGC ACTTCTGGAA CAAAACCTGC ACACCTTGGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC GGAAGAAGTA TTTATGGCGA TCCGGAATCC TCTGGAATGG
    CACTGCAAGC AGATGGATCA CTTTGTCGGA CTCAACTTCA ACTCTAACTT TAACTTTGCA
    CTAGTTGGAC ACCTCTTAAA AGGGTACAGG CATCCTTCAC CTGCCATTGT TGCAAGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTC GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CACAGAGTGT GGCTTACTTA GCAGCTCTAC TCACAGTGTC TGAAGAGGTT
    CGAAGTCGCT GTAGCCTCAA GCATAGGAAG TCCCTTCTTC TTACTGATAT TTCAATGGAA
    AATGTTCCTA TGGATACATA TCCCATTCAT CACGGTGACC CCAGCTATAG GACACTAAAG
    GAGACCCAGC CATGGTCCTC TCCCAAAGGT TCAGAAGGAT ACCTTGCAGC CACCTACCCA
    GCTGTAGGCC AAACCAGTCC CCGAGCCAGG AAATCCATGA GCTTGGATAT GGGACAGCCT
    TCTCAGGCCA ACACAAAAAA ATTGCTTGGA ACAAGAAAAA GTTTTGATCA CTTGATATCT
    GACACAAAGG CTCCTAAAAG ACAAGAAATG GAATCAGGGA TTACAACACC CCCGAAAATG
    AGAAGAGTGG CAGAGACTGA CTATGAAATG GCAGAAACCC AAAGGATTCC CTCCTCGCAG
    CAGCACCCAC ATTTACGGAA AGTCTCAGTC TCTGAATCAA ATGTTCTTTT GGATGAAGAA
    GTTCTTACCG ACCCAAAGAT CCAAGCGCTG CTTCTTACTG TTCTGGCTAC ACTGGTAAAA
    TATACTACAG ATGAATTTGA TCAACGGATT CTTTATGAAT ATCTAGCAGA GGCCAGCGTT
    GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC AATTTGTTGG ACTCTAAGAT CAATACCCTC
    TTGTCTTTAT GCCAAGATCC AAATTTGTTA AATCCAATCC ATGGAATTGT ACAGAGTGTG
    GTGTACCATG AGGAGTCCCC GCCACAGTAC CAGACATCTT ACCTGCAAAG TTTTGGTTTT
    AATGGCTTAT GGCGGTTTGC AGGACCCTTT TCAAAGCAAA CACAAATCCC TGACTATGCT
    GAGCTCATTG TTAAATTTCT TGATGCCTTG ATTGACACGT ACTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ACCCTCCTGC ACTGCAGAGC
    CAGCTTAGTA TCACTGCCAA CCTTAACCTC TCTAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGACTCGA CAAGGAGAAC GTTGAACTCT CCCCCACCGC TGGCCACTGT
    AACAGTGGAC GGACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45707
    Clone ID Related Accession (Same CDS sequence) XM_006532441.3 , XM_006532441.4
    Accession Version XM_006532441.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGC
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGCT GTGGAGCGCA CCCAGCAATA
    CGAATGGCAC CAAGTCTCAC CTTTAAAGAA AAGGTAACAA GCCTTAAATT TAAAGAAAAG
    CCTACAGACC TAGAGACAAG GAGCTACAAA TGTCTTCTTT TGTCCATGGT GAAACTGATT
    CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAGAGC
    AGTACAGCAG AACTAATTAC AGGGCTCGTA CAACTGGTTC CTCAGTCACA CATGCCAGAG
    GTCGCTCAGG AGGCTATGGA GGCTTTGCTG GTCCTTCATC AGTTAGATAG CATTGATCTC
    TGGAATCCTG ACGCTCCTGT AGAGACATTC TGGGAAATCA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA
    CGGGAAATTC TGATCTGCAG AAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGC
    TCCTGTCATT CTCTCTACCT TTATGGAGTA GGATGTGAAA TGTCTGCTAC TGGAAATACC
    ACTCAGATGT CAGTTGATCA TGATGAGTTC CTGCGTGCCT GTACTCCTGG AGCATCTCTG
    CGGAAAGGAA GAGGGAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCT
    ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCCTTAT ACATGTTTCT GTGGAATCCT
    GACACTGAAG CTGTTCTGGT TGCTATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT
    ATTCGATGTG GGGTAGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CCTCAGTCAG CAATATGATG TCAACAGGAA GAGCAGCGCT TCAGAAAAGA
    GTGATGGCCC TGCTAAGGCG CATTGAGCAC CCTACTGCAG GAAACATTGA GGCCTGGGAA
    GATACACATG CAAAGTGGGA ACAGGCTACA AAACTAATCC TTAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC AGAAAGTCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC
    CATGTCAGTG GAGGAGGCTC CATAGACTTG TCTGACACAG ACTCCCTGCA GGAGTGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT
    GGCCTAGCGA CATACAGCCC ACCTATGGGC GCTGTCAGTG AACGCAAAGG GTCTATGATT
    TCTGTAATGT CTTCTGAAGG GAATATTGAT TCACCTGTCA GCAGATTTAT GGACCGGCTT
    CTGTCCTTAA TGGTGTGTAA CCACGAGAAA GTGGGGCTTC AGATACGGAC CAATGTTAAG
    GACCTGGTGG GTCTGGAGTT GAGTCCTGCT CTGTATCCAA TGCTGTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TACTAAGTGA CAGCAATACT
    CAGTTTGTAG AGCAAACCAT AGCCATAATG AAAAACCTAC TGGATAATCA TACTGAAGGC
    AGCTCTGAGC ATCTGGGACA AGCGAGCATT GAGACAATGA TGCTAAATCT GGTCAGATAC
    GTTCGTGTTC TTGGGAATAT GGTCCATGCA ATACAAATAA AAACAAAATT GTGTCAGTTA
    GTTGAAGTCA TGATGGCAAG AAGAGATGAC CTCTCGTTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT AACAGACTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGACATAA AATGTCTTAC GAGAGATCTG GACCAAGCAA GCATGGAAGC AGTAGTTTCT
    CTCCTCGCTG GTCTTCCACT GCAGCCCGAA GAAGGAGATG GGGTGGAGCT GATGGAGGCC
    AAATCACAGT TATTCCTTAA GTACTTCACA TTATTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACGGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCCCTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC
    AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCACAAGG ATCTTCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTCGATAC ACTTGCGGAA
    ACGGTGTTGG CAGATCGATT TGAGAGACTT GTGGAACTGG TCACAATGAT GGGAGACCAG
    GGAGAACTCC CTATAGCTAT GGCTCTTGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG
    CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT TCCCGGCATT TACTCTACCA GCTGCTCTGG
    AACATGTTTT CTAAGGAGGT AGAATTGGCA GACTCCATGC AGACTCTCTT TCGAGGCAAC
    AGCTTGGCCA GTAAGATAAT GACATTCTGC TTCAAGGTGT ATGGTGCTAC TTACCTACAA
    AAGCTCTTGG ACCCTTTATT ACGAGTCATC ATCACATCTT CGGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACCC TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC
    CTCCTTCAGA TGACAGAGAA GTTCTTCCAT GCCATCATCA GCTCTTCCTC AGAATTCCCC
    TCACAGCTTC GAAGTGTCTG CCATTGTTTG TACCAGGTGG TTAGCCAGCG CTTCCCTCAG
    AACAGCATCG GTGCCGTAGG AAGTGCCATG TTTCTCAGAT TCATCAATCC TGCCATTGTC
    TCGCCGTATG AAGCAGGGAT TTTAGATAAA AAGCCACCAC CTAGAATTGA AAGGGGCTTG
    AAGTTAATGT CAAAGGTACT TCAGAGCATT GCCAATCATG TACTGTTCAC AAAAGAAGAG
    CATATGAGAC CTTTTAATGA TTTTGTGAAA AGCAACTTTG ACTTGGCACG AAGGTTTTTC
    CTAGATATAG CATCAGATTG TCCCACAAGT GATGCAGTAA ACCATAGTCT TTCCTTCATC
    AGCGATGGCA ATGTGCTTGC TTTACATCGG CTGCTTTGGA ACAATCAGGA GAAAATTGGC
    CAGTATCTTT CCAGTAACAG GGATCATAAA GCTGTTGGAA GACGACCTTT TGATAAGATG
    GCCACACTTC TCGCGTATCT GGGTCCTCCG GAGCACAAGC CTGTGGCAGA TACACACTGG
    TCCAGCCTTA ACCTTACCAG TTCAAAGTTT GAGGAATTTA TGACCAGGCA CCAGGTACAT
    GAGAAAGAAG AGTTCAAGGC CTTGAAAACG TTAAGCATCT TCTACCAAGC TGGCACCTCC
    AAAGCTGGGA ATCCTATTTT TTATTATGTT GCACGGAGGT TCAAAACTGG CCAGATCAAT
    GGTGATCTGC TGATATACCA TGTCTTGCTG ACTTTAAAGC CATATTATGC AAAGCCATAT
    GAAATTGTAG TGGACCTTAC CCATACTGGG CCTAGCAATC GCTTTAAAAC AGACTTCCTC
    TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT GCCTATGACA ACGTCTCTGC AGTCTATATC
    TATAACTGTA ACTCCTGGGT CAGGGAGTAT ACCAAATACC ATGAGCGGCT GCTGACTGGC
    CTCAAGGGCA GCAAAAGGCT CATTTTCATC GACTGTCCTG GGAAGCTGGC TGAACATATA
    GAGCATGAGC AACAGAAACT CCCTGCTGCT ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT
    TTCCACAATG CTCTCAAGTT AGCTCACAAG GACACCAAAG TTTCTATTAA GGTTGGTTCT
    ACTGCTGTTC AGGTAACTTC AGCAGAAAGA ACAAAAGTTT TGGGGCAATC CGTCTTTTTA
    AATGATATTT ACTATGCTTC TGAAATCGAA GAGATCTGCC TAGTTGATGA GAACCAGTTC
    ACTCTAACCA TTGCAAACCA AGGCACACCA CTGACCTTCA TGCACCAAGA ATGTGAAGCC
    ATTGTTCAGT CTATCATTCA TATCAGGACC CGCTGGGAGC TGTCACAACC TGACTCCATC
    CCTCAGCATA CCAAGATTCG ACCAAAGGAT GTCCCTGGGA CACTGCTTAA TATTGCGTTA
    CTGAATTTGG GCAGTTCAGA CCCTAGTTTA CGGTCTGCTG CCTATAATCT CCTGTGTGCC
    TTAACCTGCA CCTTTAATTT AAAGATTGAA GGTCAGTTGC TAGAGACATC GGGGTTATGC
    ATCCCTGCCA ACAACACACT CTTCATTGTC TCTATCAGTA AGACGCTCGC AGCCAATGAG
    CCACACCTCA CCTTAGAGTT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAATTGAAGC ACCTTTGTTT GGAATATATG ACCCCATGGC TGTCAAATCT AGTCCGTTTT
    TGTAAGCATA ATGATGATGC CAAACGACAA AGGGTTACTG CCATCCTTGA TAAGCTAATA
    ACAATGACTA TAAACGAGAA GCAGATGTAT CCTTCTATTC AAGCAAAAAT CTGGGGGAGC
    CTTGGGCAGA TCACAGACCT GCTGGATGTT GTGCTTGACA GTTTCATCAA GACCAGTGCG
    ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG GTGATGGCAG ACACAGCTGT GGCTTTAGCT
    TCTGGAAATG TGAAATTGGT GTCGAGTAAG GTTATTGGAA GGATGTGTAA AATAATTGAC
    AAGACTTGCT TATCCCCAAC TCCAACTTTA GAACAACATC TTATGTGGGA CGACATTGCC
    ATTTTAGCCC GCTACATGCT GATGCTGTCC TTCAACAACT CCCTCGATGT GGCGGCTCAT
    CTGCCCTATC TCTTCCATGT TGTCACTTTC TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA
    GCTTCCACAC ATGGGCTGCT CATCAATATC ATTCACTCTC TGTGTACTTG TTCTCAGCTT
    CACTTTAGTG AAGAGACCAA GCAAGTTTTG AGGCTCAGTC TAACAGAGTT CTCGTTACCC
    AAATTTTACT TACTGTTTGG CATTAGCAAA GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT
    TCCAGTTACC GGGACCGCTC CTTCTCCCCT GGCTCCTATG AGAGGGAGAC TTTTGCTTTG
    ACGTCCCTGG AAACAGTCAC AGAAGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT
    ATTCCAACAT GCAAGTGGCT GGATCAGTGG ACAGAACTAG CTCAAAGATT TGCGTTTCAG
    TATAACCCAT CGCTGCAGCC AAGAGCTCTT GTGGTGTTTG GCTGTATTAG CAAACGAGTG
    TCTCATGGGC AGATAAAGCA GATTATCCGA ATTCTTAGCA AGGCACTTGA AAGTTGTTTA
    AAAGGACCTG ACACTTACAA CAGTCAAGTT CTGATAGAAT CTACGGTGAT AGCACTAACA
    AAATTACAGC CGCTTCTTAA TAAGGACTCG CCCCTGCACA AAGCCCTCTT TTGGGTTGCT
    GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC TTGTATTCAG CCGGCACTGC ACTTCTGGAA
    CAAAACCTGC ACACCTTGGA CAGTCTCCGG ATATTCAATG ACAAGAGTCC GGAAGAAGTA
    TTTATGGCGA TCCGGAATCC TCTGGAATGG CACTGCAAGC AGATGGATCA CTTTGTCGGA
    CTCAACTTCA ACTCTAACTT TAACTTTGCA CTAGTTGGAC ACCTCTTAAA AGGGTACAGG
    CATCCTTCAC CTGCCATTGT TGCAAGAACA GTCAGAATTT TGCATACACT ACTAACTCTC
    GTTAACAAAC ATAGAAATTG TGACAAATTT GAGGTGAATA CACAGAGTGT GGCTTACTTA
    GCAGCTCTAC TCACAGTGTC TGAAGAGGTT CGAAGTCGCT GTAGCCTCAA GCATAGGAAG
    TCCCTTCTTC TTACTGATAT TTCAATGGAA AATGTTCCTA TGGATACATA TCCCATTCAT
    CACGGTGACC CCAGCTATAG GACACTAAAG GAGACCCAGC CATGGTCCTC TCCCAAAGGT
    TCAGAAGGAT ACCTTGCAGC CACCTACCCA GCTGTAGGCC AAACCAGTCC CCGAGCCAGG
    AAATCCATGA GCTTGGATAT GGGACAGCCT TCTCAGGCCA ACACAAAAAA ATTGCTTGGA
    ACAAGAAAAA GTTTTGATCA CTTGATATCT GACACAAAGG CTCCTAAAAG ACAAGAAATG
    GAATCAGGGA TTACAACACC CCCGAAAATG AGAAGAGTGG CAGAGACTGA CTATGAAATG
    GAAACCCAAA GGATTCCCTC CTCGCAGCAG CACCCACATT TACGGAAAGT CTCAGTCTCT
    GAATCAAATG TTCTTTTGGA TGAAGAAGTT CTTACCGACC CAAAGATCCA AGCGCTGCTT
    CTTACTGTTC TGGCTACACT GGTAAAATAT ACTACAGATG AATTTGATCA ACGGATTCTT
    TATGAATATC TAGCAGAGGC CAGCGTTGTG TTTCCCAAAG TGTTTCCTGT TGTGCACAAT
    TTGTTGGACT CTAAGATCAA TACCCTCTTG TCTTTATGCC AAGATCCAAA TTTGTTAAAT
    CCAATCCATG GAATTGTACA GAGTGTGGTG TACCATGAGG AGTCCCCGCC ACAGTACCAG
    ACATCTTACC TGCAAAGTTT TGGTTTTAAT GGCTTATGGC GGTTTGCAGG ACCCTTTTCA
    AAGCAAACAC AAATCCCTGA CTATGCTGAG CTCATTGTTA AATTTCTTGA TGCCTTGATT
    GACACGTACT TGCCTGGAAT TGATGAAGAA ACCAGTGAGG AGTCCCTCCT GACACCCACA
    TCTCCTTACC CTCCTGCACT GCAGAGCCAG CTTAGTATCA CTGCCAACCT TAACCTCTCT
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GACTCGACAA GGAGAACGTT
    GAACTCTCCC CCACCGCTGG CCACTGTAAC AGTGGACGGA CTCGCCATGG ATCCGCAAGC
    CAAGTGCAGA AGCAAAGAAG TGCTGGCAGT TTCAAACGTA ATAGCATTAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532504.1
    CDS1612..10074
    Translation

    Target ORF information:

    RefSeq Version XM_006532441.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromatosis 1 (Nf1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532441.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGC
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGCT GTGGAGCGCA CCCAGCAATA
    CGAATGGCAC CAAGTCTCAC CTTTAAAGAA AAGGTAACAA GCCTTAAATT TAAAGAAAAG
    CCTACAGACC TAGAGACAAG GAGCTACAAA TGTCTTCTTT TGTCCATGGT GAAACTGATT
    CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAGAGC
    AGTACAGCAG AACTAATTAC AGGGCTCGTA CAACTGGTTC CTCAGTCACA CATGCCAGAG
    GTCGCTCAGG AGGCTATGGA GGCTTTGCTG GTCCTTCATC AGTTAGATAG CATTGATCTC
    TGGAATCCTG ACGCTCCTGT AGAGACATTC TGGGAAATCA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA
    CGGGAAATTC TGATCTGCAG AAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGC
    TCCTGTCATT CTCTCTACCT TTATGGAGTA GGATGTGAAA TGTCTGCTAC TGGAAATACC
    ACTCAGATGT CAGTTGATCA TGATGAGTTC CTGCGTGCCT GTACTCCTGG AGCATCTCTG
    CGGAAAGGAA GAGGGAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCT
    ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCCTTAT ACATGTTTCT GTGGAATCCT
    GACACTGAAG CTGTTCTGGT TGCTATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT
    ATTCGATGTG GGGTAGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CCTCAGTCAG CAATATGATG TCAACAGGAA GAGCAGCGCT TCAGAAAAGA
    GTGATGGCCC TGCTAAGGCG CATTGAGCAC CCTACTGCAG GAAACATTGA GGCCTGGGAA
    GATACACATG CAAAGTGGGA ACAGGCTACA AAACTAATCC TTAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC AGAAAGTCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC
    CATGTCAGTG GAGGAGGCTC CATAGACTTG TCTGACACAG ACTCCCTGCA GGAGTGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT
    GGCCTAGCGA CATACAGCCC ACCTATGGGC GCTGTCAGTG AACGCAAAGG GTCTATGATT
    TCTGTAATGT CTTCTGAAGG GAATATTGAT TCACCTGTCA GCAGATTTAT GGACCGGCTT
    CTGTCCTTAA TGGTGTGTAA CCACGAGAAA GTGGGGCTTC AGATACGGAC CAATGTTAAG
    GACCTGGTGG GTCTGGAGTT GAGTCCTGCT CTGTATCCAA TGCTGTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TACTAAGTGA CAGCAATACT
    CAGTTTGTAG AGCAAACCAT AGCCATAATG AAAAACCTAC TGGATAATCA TACTGAAGGC
    AGCTCTGAGC ATCTGGGACA AGCGAGCATT GAGACAATGA TGCTAAATCT GGTCAGATAC
    GTTCGTGTTC TTGGGAATAT GGTCCATGCA ATACAAATAA AAACAAAATT GTGTCAGTTA
    GTTGAAGTCA TGATGGCAAG AAGAGATGAC CTCTCGTTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT AACAGACTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGACATAA AATGTCTTAC GAGAGATCTG GACCAAGCAA GCATGGAAGC AGTAGTTTCT
    CTCCTCGCTG GTCTTCCACT GCAGCCCGAA GAAGGAGATG GGGTGGAGCT GATGGAGGCC
    AAATCACAGT TATTCCTTAA GTACTTCACA TTATTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACGGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCCCTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC
    AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCACAAGG ATCTTCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTCGATAC ACTTGCGGAA
    ACGGTGTTGG CAGATCGATT TGAGAGACTT GTGGAACTGG TCACAATGAT GGGAGACCAG
    GGAGAACTCC CTATAGCTAT GGCTCTTGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG
    CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT TCCCGGCATT TACTCTACCA GCTGCTCTGG
    AACATGTTTT CTAAGGAGGT AGAATTGGCA GACTCCATGC AGACTCTCTT TCGAGGCAAC
    AGCTTGGCCA GTAAGATAAT GACATTCTGC TTCAAGGTGT ATGGTGCTAC TTACCTACAA
    AAGCTCTTGG ACCCTTTATT ACGAGTCATC ATCACATCTT CGGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACCC TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC
    CTCCTTCAGA TGACAGAGAA GTTCTTCCAT GCCATCATCA GCTCTTCCTC AGAATTCCCC
    TCACAGCTTC GAAGTGTCTG CCATTGTTTG TACCAGGTGG TTAGCCAGCG CTTCCCTCAG
    AACAGCATCG GTGCCGTAGG AAGTGCCATG TTTCTCAGAT TCATCAATCC TGCCATTGTC
    TCGCCGTATG AAGCAGGGAT TTTAGATAAA AAGCCACCAC CTAGAATTGA AAGGGGCTTG
    AAGTTAATGT CAAAGGTACT TCAGAGCATT GCCAATCATG TACTGTTCAC AAAAGAAGAG
    CATATGAGAC CTTTTAATGA TTTTGTGAAA AGCAACTTTG ACTTGGCACG AAGGTTTTTC
    CTAGATATAG CATCAGATTG TCCCACAAGT GATGCAGTAA ACCATAGTCT TTCCTTCATC
    AGCGATGGCA ATGTGCTTGC TTTACATCGG CTGCTTTGGA ACAATCAGGA GAAAATTGGC
    CAGTATCTTT CCAGTAACAG GGATCATAAA GCTGTTGGAA GACGACCTTT TGATAAGATG
    GCCACACTTC TCGCGTATCT GGGTCCTCCG GAGCACAAGC CTGTGGCAGA TACACACTGG
    TCCAGCCTTA ACCTTACCAG TTCAAAGTTT GAGGAATTTA TGACCAGGCA CCAGGTACAT
    GAGAAAGAAG AGTTCAAGGC CTTGAAAACG TTAAGCATCT TCTACCAAGC TGGCACCTCC
    AAAGCTGGGA ATCCTATTTT TTATTATGTT GCACGGAGGT TCAAAACTGG CCAGATCAAT
    GGTGATCTGC TGATATACCA TGTCTTGCTG ACTTTAAAGC CATATTATGC AAAGCCATAT
    GAAATTGTAG TGGACCTTAC CCATACTGGG CCTAGCAATC GCTTTAAAAC AGACTTCCTC
    TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT GCCTATGACA ACGTCTCTGC AGTCTATATC
    TATAACTGTA ACTCCTGGGT CAGGGAGTAT ACCAAATACC ATGAGCGGCT GCTGACTGGC
    CTCAAGGGCA GCAAAAGGCT CATTTTCATC GACTGTCCTG GGAAGCTGGC TGAACATATA
    GAGCATGAGC AACAGAAACT CCCTGCTGCT ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT
    TTCCACAATG CTCTCAAGTT AGCTCACAAG GACACCAAAG TTTCTATTAA GGTTGGTTCT
    ACTGCTGTTC AGGTAACTTC AGCAGAAAGA ACAAAAGTTT TGGGGCAATC CGTCTTTTTA
    AATGATATTT ACTATGCTTC TGAAATCGAA GAGATCTGCC TAGTTGATGA GAACCAGTTC
    ACTCTAACCA TTGCAAACCA AGGCACACCA CTGACCTTCA TGCACCAAGA ATGTGAAGCC
    ATTGTTCAGT CTATCATTCA TATCAGGACC CGCTGGGAGC TGTCACAACC TGACTCCATC
    CCTCAGCATA CCAAGATTCG ACCAAAGGAT GTCCCTGGGA CACTGCTTAA TATTGCGTTA
    CTGAATTTGG GCAGTTCAGA CCCTAGTTTA CGGTCTGCTG CCTATAATCT CCTGTGTGCC
    TTAACCTGCA CCTTTAATTT AAAGATTGAA GGTCAGTTGC TAGAGACATC GGGGTTATGC
    ATCCCTGCCA ACAACACACT CTTCATTGTC TCTATCAGTA AGACGCTCGC AGCCAATGAG
    CCACACCTCA CCTTAGAGTT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAATTGAAGC ACCTTTGTTT GGAATATATG ACCCCATGGC TGTCAAATCT AGTCCGTTTT
    TGTAAGCATA ATGATGATGC CAAACGACAA AGGGTTACTG CCATCCTTGA TAAGCTAATA
    ACAATGACTA TAAACGAGAA GCAGATGTAT CCTTCTATTC AAGCAAAAAT CTGGGGGAGC
    CTTGGGCAGA TCACAGACCT GCTGGATGTT GTGCTTGACA GTTTCATCAA GACCAGTGCG
    ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG GTGATGGCAG ACACAGCTGT GGCTTTAGCT
    TCTGGAAATG TGAAATTGGT GTCGAGTAAG GTTATTGGAA GGATGTGTAA AATAATTGAC
    AAGACTTGCT TATCCCCAAC TCCAACTTTA GAACAACATC TTATGTGGGA CGACATTGCC
    ATTTTAGCCC GCTACATGCT GATGCTGTCC TTCAACAACT CCCTCGATGT GGCGGCTCAT
    CTGCCCTATC TCTTCCATGT TGTCACTTTC TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA
    GCTTCCACAC ATGGGCTGCT CATCAATATC ATTCACTCTC TGTGTACTTG TTCTCAGCTT
    CACTTTAGTG AAGAGACCAA GCAAGTTTTG AGGCTCAGTC TAACAGAGTT CTCGTTACCC
    AAATTTTACT TACTGTTTGG CATTAGCAAA GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT
    TCCAGTTACC GGGACCGCTC CTTCTCCCCT GGCTCCTATG AGAGGGAGAC TTTTGCTTTG
    ACGTCCCTGG AAACAGTCAC AGAAGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT
    ATTCCAACAT GCAAGTGGCT GGATCAGTGG ACAGAACTAG CTCAAAGATT TGCGTTTCAG
    TATAACCCAT CGCTGCAGCC AAGAGCTCTT GTGGTGTTTG GCTGTATTAG CAAACGAGTG
    TCTCATGGGC AGATAAAGCA GATTATCCGA ATTCTTAGCA AGGCACTTGA AAGTTGTTTA
    AAAGGACCTG ACACTTACAA CAGTCAAGTT CTGATAGAAT CTACGGTGAT AGCACTAACA
    AAATTACAGC CGCTTCTTAA TAAGGACTCG CCCCTGCACA AAGCCCTCTT TTGGGTTGCT
    GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC TTGTATTCAG CCGGCACTGC ACTTCTGGAA
    CAAAACCTGC ACACCTTGGA CAGTCTCCGG ATATTCAATG ACAAGAGTCC GGAAGAAGTA
    TTTATGGCGA TCCGGAATCC TCTGGAATGG CACTGCAAGC AGATGGATCA CTTTGTCGGA
    CTCAACTTCA ACTCTAACTT TAACTTTGCA CTAGTTGGAC ACCTCTTAAA AGGGTACAGG
    CATCCTTCAC CTGCCATTGT TGCAAGAACA GTCAGAATTT TGCATACACT ACTAACTCTC
    GTTAACAAAC ATAGAAATTG TGACAAATTT GAGGTGAATA CACAGAGTGT GGCTTACTTA
    GCAGCTCTAC TCACAGTGTC TGAAGAGGTT CGAAGTCGCT GTAGCCTCAA GCATAGGAAG
    TCCCTTCTTC TTACTGATAT TTCAATGGAA AATGTTCCTA TGGATACATA TCCCATTCAT
    CACGGTGACC CCAGCTATAG GACACTAAAG GAGACCCAGC CATGGTCCTC TCCCAAAGGT
    TCAGAAGGAT ACCTTGCAGC CACCTACCCA GCTGTAGGCC AAACCAGTCC CCGAGCCAGG
    AAATCCATGA GCTTGGATAT GGGACAGCCT TCTCAGGCCA ACACAAAAAA ATTGCTTGGA
    ACAAGAAAAA GTTTTGATCA CTTGATATCT GACACAAAGG CTCCTAAAAG ACAAGAAATG
    GAATCAGGGA TTACAACACC CCCGAAAATG AGAAGAGTGG CAGAGACTGA CTATGAAATG
    GAAACCCAAA GGATTCCCTC CTCGCAGCAG CACCCACATT TACGGAAAGT CTCAGTCTCT
    GAATCAAATG TTCTTTTGGA TGAAGAAGTT CTTACCGACC CAAAGATCCA AGCGCTGCTT
    CTTACTGTTC TGGCTACACT GGTAAAATAT ACTACAGATG AATTTGATCA ACGGATTCTT
    TATGAATATC TAGCAGAGGC CAGCGTTGTG TTTCCCAAAG TGTTTCCTGT TGTGCACAAT
    TTGTTGGACT CTAAGATCAA TACCCTCTTG TCTTTATGCC AAGATCCAAA TTTGTTAAAT
    CCAATCCATG GAATTGTACA GAGTGTGGTG TACCATGAGG AGTCCCCGCC ACAGTACCAG
    ACATCTTACC TGCAAAGTTT TGGTTTTAAT GGCTTATGGC GGTTTGCAGG ACCCTTTTCA
    AAGCAAACAC AAATCCCTGA CTATGCTGAG CTCATTGTTA AATTTCTTGA TGCCTTGATT
    GACACGTACT TGCCTGGAAT TGATGAAGAA ACCAGTGAGG AGTCCCTCCT GACACCCACA
    TCTCCTTACC CTCCTGCACT GCAGAGCCAG CTTAGTATCA CTGCCAACCT TAACCTCTCT
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GACTCGACAA GGAGAACGTT
    GAACTCTCCC CCACCGCTGG CCACTGTAAC AGTGGACGGA CTCGCCATGG ATCCGCAAGC
    CAAGTGCAGA AGCAAAGAAG TGCTGGCAGT TTCAAACGTA ATAGCATTAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45707
    Clone ID Related Accession (Same CDS sequence) XM_006532441.3 , XM_006532441.4
    Accession Version XM_006532441.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532441.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    541
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGC
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGCT GTGGAGCGCA CCCAGCAATA
    CGAATGGCAC CAAGTCTCAC CTTTAAAGAA AAGGTAACAA GCCTTAAATT TAAAGAAAAG
    CCTACAGACC TAGAGACAAG GAGCTACAAA TGTCTTCTTT TGTCCATGGT GAAACTGATT
    CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAGAGC
    AGTACAGCAG AACTAATTAC AGGGCTCGTA CAACTGGTTC CTCAGTCACA CATGCCAGAG
    GTCGCTCAGG AGGCTATGGA GGCTTTGCTG GTCCTTCATC AGTTAGATAG CATTGATCTC
    TGGAATCCTG ACGCTCCTGT AGAGACATTC TGGGAAATCA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA
    CGGGAAATTC TGATCTGCAG AAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGC
    TCCTGTCATT CTCTCTACCT TTATGGAGTA GGATGTGAAA TGTCTGCTAC TGGAAATACC
    ACTCAGATGT CAGTTGATCA TGATGAGTTC CTGCGTGCCT GTACTCCTGG AGCATCTCTG
    CGGAAAGGAA GAGGGAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCT
    ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCCTTAT ACATGTTTCT GTGGAATCCT
    GACACTGAAG CTGTTCTGGT TGCTATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT
    ATTCGATGTG GGGTAGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CCTCAGTCAG CAATATGATG TCAACAGGAA GAGCAGCGCT TCAGAAAAGA
    GTGATGGCCC TGCTAAGGCG CATTGAGCAC CCTACTGCAG GAAACATTGA GGCCTGGGAA
    GATACACATG CAAAGTGGGA ACAGGCTACA AAACTAATCC TTAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC AGAAAGTCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC
    CATGTCAGTG GAGGAGGCTC CATAGACTTG TCTGACACAG ACTCCCTGCA GGAGTGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT
    GGCCTAGCGA CATACAGCCC ACCTATGGGC GCTGTCAGTG AACGCAAAGG GTCTATGATT
    TCTGTAATGT CTTCTGAAGG GAATATTGAT TCACCTGTCA GCAGATTTAT GGACCGGCTT
    CTGTCCTTAA TGGTGTGTAA CCACGAGAAA GTGGGGCTTC AGATACGGAC CAATGTTAAG
    GACCTGGTGG GTCTGGAGTT GAGTCCTGCT CTGTATCCAA TGCTGTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TACTAAGTGA CAGCAATACT
    CAGTTTGTAG AGCAAACCAT AGCCATAATG AAAAACCTAC TGGATAATCA TACTGAAGGC
    AGCTCTGAGC ATCTGGGACA AGCGAGCATT GAGACAATGA TGCTAAATCT GGTCAGATAC
    GTTCGTGTTC TTGGGAATAT GGTCCATGCA ATACAAATAA AAACAAAATT GTGTCAGTTA
    GTTGAAGTCA TGATGGCAAG AAGAGATGAC CTCTCGTTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT AACAGACTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGACATAA AATGTCTTAC GAGAGATCTG GACCAAGCAA GCATGGAAGC AGTAGTTTCT
    CTCCTCGCTG GTCTTCCACT GCAGCCCGAA GAAGGAGATG GGGTGGAGCT GATGGAGGCC
    AAATCACAGT TATTCCTTAA GTACTTCACA TTATTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACGGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCCCTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC
    AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCACAAGG ATCTTCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTCGATAC ACTTGCGGAA
    ACGGTGTTGG CAGATCGATT TGAGAGACTT GTGGAACTGG TCACAATGAT GGGAGACCAG
    GGAGAACTCC CTATAGCTAT GGCTCTTGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG
    CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT TCCCGGCATT TACTCTACCA GCTGCTCTGG
    AACATGTTTT CTAAGGAGGT AGAATTGGCA GACTCCATGC AGACTCTCTT TCGAGGCAAC
    AGCTTGGCCA GTAAGATAAT GACATTCTGC TTCAAGGTGT ATGGTGCTAC TTACCTACAA
    AAGCTCTTGG ACCCTTTATT ACGAGTCATC ATCACATCTT CGGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACCC TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC
    CTCCTTCAGA TGACAGAGAA GTTCTTCCAT GCCATCATCA GCTCTTCCTC AGAATTCCCC
    TCACAGCTTC GAAGTGTCTG CCATTGTTTG TACCAGGTGG TTAGCCAGCG CTTCCCTCAG
    AACAGCATCG GTGCCGTAGG AAGTGCCATG TTTCTCAGAT TCATCAATCC TGCCATTGTC
    TCGCCGTATG AAGCAGGGAT TTTAGATAAA AAGCCACCAC CTAGAATTGA AAGGGGCTTG
    AAGTTAATGT CAAAGGTACT TCAGAGCATT GCCAATCATG TACTGTTCAC AAAAGAAGAG
    CATATGAGAC CTTTTAATGA TTTTGTGAAA AGCAACTTTG ACTTGGCACG AAGGTTTTTC
    CTAGATATAG CATCAGATTG TCCCACAAGT GATGCAGTAA ACCATAGTCT TTCCTTCATC
    AGCGATGGCA ATGTGCTTGC TTTACATCGG CTGCTTTGGA ACAATCAGGA GAAAATTGGC
    CAGTATCTTT CCAGTAACAG GGATCATAAA GCTGTTGGAA GACGACCTTT TGATAAGATG
    GCCACACTTC TCGCGTATCT GGGTCCTCCG GAGCACAAGC CTGTGGCAGA TACACACTGG
    TCCAGCCTTA ACCTTACCAG TTCAAAGTTT GAGGAATTTA TGACCAGGCA CCAGGTACAT
    GAGAAAGAAG AGTTCAAGGC CTTGAAAACG TTAAGCATCT TCTACCAAGC TGGCACCTCC
    AAAGCTGGGA ATCCTATTTT TTATTATGTT GCACGGAGGT TCAAAACTGG CCAGATCAAT
    GGTGATCTGC TGATATACCA TGTCTTGCTG ACTTTAAAGC CATATTATGC AAAGCCATAT
    GAAATTGTAG TGGACCTTAC CCATACTGGG CCTAGCAATC GCTTTAAAAC AGACTTCCTC
    TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT GCCTATGACA ACGTCTCTGC AGTCTATATC
    TATAACTGTA ACTCCTGGGT CAGGGAGTAT ACCAAATACC ATGAGCGGCT GCTGACTGGC
    CTCAAGGGCA GCAAAAGGCT CATTTTCATC GACTGTCCTG GGAAGCTGGC TGAACATATA
    GAGCATGAGC AACAGAAACT CCCTGCTGCT ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT
    TTCCACAATG CTCTCAAGTT AGCTCACAAG GACACCAAAG TTTCTATTAA GGTTGGTTCT
    ACTGCTGTTC AGGTAACTTC AGCAGAAAGA ACAAAAGTTT TGGGGCAATC CGTCTTTTTA
    AATGATATTT ACTATGCTTC TGAAATCGAA GAGATCTGCC TAGTTGATGA GAACCAGTTC
    ACTCTAACCA TTGCAAACCA AGGCACACCA CTGACCTTCA TGCACCAAGA ATGTGAAGCC
    ATTGTTCAGT CTATCATTCA TATCAGGACC CGCTGGGAGC TGTCACAACC TGACTCCATC
    CCTCAGCATA CCAAGATTCG ACCAAAGGAT GTCCCTGGGA CACTGCTTAA TATTGCGTTA
    CTGAATTTGG GCAGTTCAGA CCCTAGTTTA CGGTCTGCTG CCTATAATCT CCTGTGTGCC
    TTAACCTGCA CCTTTAATTT AAAGATTGAA GGTCAGTTGC TAGAGACATC GGGGTTATGC
    ATCCCTGCCA ACAACACACT CTTCATTGTC TCTATCAGTA AGACGCTCGC AGCCAATGAG
    CCACACCTCA CCTTAGAGTT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAATTGAAGC ACCTTTGTTT GGAATATATG ACCCCATGGC TGTCAAATCT AGTCCGTTTT
    TGTAAGCATA ATGATGATGC CAAACGACAA AGGGTTACTG CCATCCTTGA TAAGCTAATA
    ACAATGACTA TAAACGAGAA GCAGATGTAT CCTTCTATTC AAGCAAAAAT CTGGGGGAGC
    CTTGGGCAGA TCACAGACCT GCTGGATGTT GTGCTTGACA GTTTCATCAA GACCAGTGCG
    ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG GTGATGGCAG ACACAGCTGT GGCTTTAGCT
    TCTGGAAATG TGAAATTGGT GTCGAGTAAG GTTATTGGAA GGATGTGTAA AATAATTGAC
    AAGACTTGCT TATCCCCAAC TCCAACTTTA GAACAACATC TTATGTGGGA CGACATTGCC
    ATTTTAGCCC GCTACATGCT GATGCTGTCC TTCAACAACT CCCTCGATGT GGCGGCTCAT
    CTGCCCTATC TCTTCCATGT TGTCACTTTC TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA
    GCTTCCACAC ATGGGCTGCT CATCAATATC ATTCACTCTC TGTGTACTTG TTCTCAGCTT
    CACTTTAGTG AAGAGACCAA GCAAGTTTTG AGGCTCAGTC TAACAGAGTT CTCGTTACCC
    AAATTTTACT TACTGTTTGG CATTAGCAAA GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT
    TCCAGTTACC GGGACCGCTC CTTCTCCCCT GGCTCCTATG AGAGGGAGAC TTTTGCTTTG
    ACGTCCCTGG AAACAGTCAC AGAAGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT
    ATTCCAACAT GCAAGTGGCT GGATCAGTGG ACAGAACTAG CTCAAAGATT TGCGTTTCAG
    TATAACCCAT CGCTGCAGCC AAGAGCTCTT GTGGTGTTTG GCTGTATTAG CAAACGAGTG
    TCTCATGGGC AGATAAAGCA GATTATCCGA ATTCTTAGCA AGGCACTTGA AAGTTGTTTA
    AAAGGACCTG ACACTTACAA CAGTCAAGTT CTGATAGAAT CTACGGTGAT AGCACTAACA
    AAATTACAGC CGCTTCTTAA TAAGGACTCG CCCCTGCACA AAGCCCTCTT TTGGGTTGCT
    GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC TTGTATTCAG CCGGCACTGC ACTTCTGGAA
    CAAAACCTGC ACACCTTGGA CAGTCTCCGG ATATTCAATG ACAAGAGTCC GGAAGAAGTA
    TTTATGGCGA TCCGGAATCC TCTGGAATGG CACTGCAAGC AGATGGATCA CTTTGTCGGA
    CTCAACTTCA ACTCTAACTT TAACTTTGCA CTAGTTGGAC ACCTCTTAAA AGGGTACAGG
    CATCCTTCAC CTGCCATTGT TGCAAGAACA GTCAGAATTT TGCATACACT ACTAACTCTC
    GTTAACAAAC ATAGAAATTG TGACAAATTT GAGGTGAATA CACAGAGTGT GGCTTACTTA
    GCAGCTCTAC TCACAGTGTC TGAAGAGGTT CGAAGTCGCT GTAGCCTCAA GCATAGGAAG
    TCCCTTCTTC TTACTGATAT TTCAATGGAA AATGTTCCTA TGGATACATA TCCCATTCAT
    CACGGTGACC CCAGCTATAG GACACTAAAG GAGACCCAGC CATGGTCCTC TCCCAAAGGT
    TCAGAAGGAT ACCTTGCAGC CACCTACCCA GCTGTAGGCC AAACCAGTCC CCGAGCCAGG
    AAATCCATGA GCTTGGATAT GGGACAGCCT TCTCAGGCCA ACACAAAAAA ATTGCTTGGA
    ACAAGAAAAA GTTTTGATCA CTTGATATCT GACACAAAGG CTCCTAAAAG ACAAGAAATG
    GAATCAGGGA TTACAACACC CCCGAAAATG AGAAGAGTGG CAGAGACTGA CTATGAAATG
    GAAACCCAAA GGATTCCCTC CTCGCAGCAG CACCCACATT TACGGAAAGT CTCAGTCTCT
    GAATCAAATG TTCTTTTGGA TGAAGAAGTT CTTACCGACC CAAAGATCCA AGCGCTGCTT
    CTTACTGTTC TGGCTACACT GGTAAAATAT ACTACAGATG AATTTGATCA ACGGATTCTT
    TATGAATATC TAGCAGAGGC CAGCGTTGTG TTTCCCAAAG TGTTTCCTGT TGTGCACAAT
    TTGTTGGACT CTAAGATCAA TACCCTCTTG TCTTTATGCC AAGATCCAAA TTTGTTAAAT
    CCAATCCATG GAATTGTACA GAGTGTGGTG TACCATGAGG AGTCCCCGCC ACAGTACCAG
    ACATCTTACC TGCAAAGTTT TGGTTTTAAT GGCTTATGGC GGTTTGCAGG ACCCTTTTCA
    AAGCAAACAC AAATCCCTGA CTATGCTGAG CTCATTGTTA AATTTCTTGA TGCCTTGATT
    GACACGTACT TGCCTGGAAT TGATGAAGAA ACCAGTGAGG AGTCCCTCCT GACACCCACA
    TCTCCTTACC CTCCTGCACT GCAGAGCCAG CTTAGTATCA CTGCCAACCT TAACCTCTCT
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GACTCGACAA GGAGAACGTT
    GAACTCTCCC CCACCGCTGG CCACTGTAAC AGTGGACGGA CTCGCCATGG ATCCGCAAGC
    CAAGTGCAGA AGCAAAGAAG TGCTGGCAGT TTCAAACGTA ATAGCATTAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532504.1
    CDS407..8869
    Translation

    Target ORF information:

    RefSeq Version XM_006532441.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromin 1 (Nf1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532441.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGC
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGCT GTGGAGCGCA CCCAGCAATA
    CGAATGGCAC CAAGTCTCAC CTTTAAAGAA AAGGTAACAA GCCTTAAATT TAAAGAAAAG
    CCTACAGACC TAGAGACAAG GAGCTACAAA TGTCTTCTTT TGTCCATGGT GAAACTGATT
    CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAGAGC
    AGTACAGCAG AACTAATTAC AGGGCTCGTA CAACTGGTTC CTCAGTCACA CATGCCAGAG
    GTCGCTCAGG AGGCTATGGA GGCTTTGCTG GTCCTTCATC AGTTAGATAG CATTGATCTC
    TGGAATCCTG ACGCTCCTGT AGAGACATTC TGGGAAATCA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA
    CGGGAAATTC TGATCTGCAG AAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGC
    TCCTGTCATT CTCTCTACCT TTATGGAGTA GGATGTGAAA TGTCTGCTAC TGGAAATACC
    ACTCAGATGT CAGTTGATCA TGATGAGTTC CTGCGTGCCT GTACTCCTGG AGCATCTCTG
    CGGAAAGGAA GAGGGAATTC CTCCATGGAT AGCACAGCAG GGTGCAGTGG AACCCCACCT
    ATATGCCGAC AAGCCCAGAC CAAGCTAGAA GTGGCCTTAT ACATGTTTCT GTGGAATCCT
    GACACTGAAG CTGTTCTGGT TGCTATGTCC TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT
    ATTCGATGTG GGGTAGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CCTCAGTCAG CAATATGATG TCAACAGGAA GAGCAGCGCT TCAGAAAAGA
    GTGATGGCCC TGCTAAGGCG CATTGAGCAC CCTACTGCAG GAAACATTGA GGCCTGGGAA
    GATACACATG CAAAGTGGGA ACAGGCTACA AAACTAATCC TTAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC AGAAAGTCTT CATAAGACCA TTGTTAAGAG ACGGATGTCC
    CATGTCAGTG GAGGAGGCTC CATAGACTTG TCTGACACAG ACTCCCTGCA GGAGTGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGT GGGGTGTGCC TGCAACAGAG AAGCAGCTCT
    GGCCTAGCGA CATACAGCCC ACCTATGGGC GCTGTCAGTG AACGCAAAGG GTCTATGATT
    TCTGTAATGT CTTCTGAAGG GAATATTGAT TCACCTGTCA GCAGATTTAT GGACCGGCTT
    CTGTCCTTAA TGGTGTGTAA CCACGAGAAA GTGGGGCTTC AGATACGGAC CAATGTTAAG
    GACCTGGTGG GTCTGGAGTT GAGTCCTGCT CTGTATCCAA TGCTGTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCTCAA GGACAGGTAT TACTAAGTGA CAGCAATACT
    CAGTTTGTAG AGCAAACCAT AGCCATAATG AAAAACCTAC TGGATAATCA TACTGAAGGC
    AGCTCTGAGC ATCTGGGACA AGCGAGCATT GAGACAATGA TGCTAAATCT GGTCAGATAC
    GTTCGTGTTC TTGGGAATAT GGTCCATGCA ATACAAATAA AAACAAAATT GTGTCAGTTA
    GTTGAAGTCA TGATGGCAAG AAGAGATGAC CTCTCGTTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT AACAGACTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGACATAA AATGTCTTAC GAGAGATCTG GACCAAGCAA GCATGGAAGC AGTAGTTTCT
    CTCCTCGCTG GTCTTCCACT GCAGCCCGAA GAAGGAGATG GGGTGGAGCT GATGGAGGCC
    AAATCACAGT TATTCCTTAA GTACTTCACA TTATTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACGGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCCCTGA GGCACTGTAC AGTGCTTGCA ATGTCAAACT TACTCAATGC TAATGTGGAC
    AGTGGACTCA TGCACTCCAT AGGCTTAGGT TATCACAAGG ATCTTCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGAACAG AATTCGATAC ACTTGCGGAA
    ACGGTGTTGG CAGATCGATT TGAGAGACTT GTGGAACTGG TCACAATGAT GGGAGACCAG
    GGAGAACTCC CTATAGCTAT GGCTCTTGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAG
    CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT TCCCGGCATT TACTCTACCA GCTGCTCTGG
    AACATGTTTT CTAAGGAGGT AGAATTGGCA GACTCCATGC AGACTCTCTT TCGAGGCAAC
    AGCTTGGCCA GTAAGATAAT GACATTCTGC TTCAAGGTGT ATGGTGCTAC TTACCTACAA
    AAGCTCTTGG ACCCTTTATT ACGAGTCATC ATCACATCTT CGGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACCC TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC
    CTCCTTCAGA TGACAGAGAA GTTCTTCCAT GCCATCATCA GCTCTTCCTC AGAATTCCCC
    TCACAGCTTC GAAGTGTCTG CCATTGTTTG TACCAGGTGG TTAGCCAGCG CTTCCCTCAG
    AACAGCATCG GTGCCGTAGG AAGTGCCATG TTTCTCAGAT TCATCAATCC TGCCATTGTC
    TCGCCGTATG AAGCAGGGAT TTTAGATAAA AAGCCACCAC CTAGAATTGA AAGGGGCTTG
    AAGTTAATGT CAAAGGTACT TCAGAGCATT GCCAATCATG TACTGTTCAC AAAAGAAGAG
    CATATGAGAC CTTTTAATGA TTTTGTGAAA AGCAACTTTG ACTTGGCACG AAGGTTTTTC
    CTAGATATAG CATCAGATTG TCCCACAAGT GATGCAGTAA ACCATAGTCT TTCCTTCATC
    AGCGATGGCA ATGTGCTTGC TTTACATCGG CTGCTTTGGA ACAATCAGGA GAAAATTGGC
    CAGTATCTTT CCAGTAACAG GGATCATAAA GCTGTTGGAA GACGACCTTT TGATAAGATG
    GCCACACTTC TCGCGTATCT GGGTCCTCCG GAGCACAAGC CTGTGGCAGA TACACACTGG
    TCCAGCCTTA ACCTTACCAG TTCAAAGTTT GAGGAATTTA TGACCAGGCA CCAGGTACAT
    GAGAAAGAAG AGTTCAAGGC CTTGAAAACG TTAAGCATCT TCTACCAAGC TGGCACCTCC
    AAAGCTGGGA ATCCTATTTT TTATTATGTT GCACGGAGGT TCAAAACTGG CCAGATCAAT
    GGTGATCTGC TGATATACCA TGTCTTGCTG ACTTTAAAGC CATATTATGC AAAGCCATAT
    GAAATTGTAG TGGACCTTAC CCATACTGGG CCTAGCAATC GCTTTAAAAC AGACTTCCTC
    TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT GCCTATGACA ACGTCTCTGC AGTCTATATC
    TATAACTGTA ACTCCTGGGT CAGGGAGTAT ACCAAATACC ATGAGCGGCT GCTGACTGGC
    CTCAAGGGCA GCAAAAGGCT CATTTTCATC GACTGTCCTG GGAAGCTGGC TGAACATATA
    GAGCATGAGC AACAGAAACT CCCTGCTGCT ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT
    TTCCACAATG CTCTCAAGTT AGCTCACAAG GACACCAAAG TTTCTATTAA GGTTGGTTCT
    ACTGCTGTTC AGGTAACTTC AGCAGAAAGA ACAAAAGTTT TGGGGCAATC CGTCTTTTTA
    AATGATATTT ACTATGCTTC TGAAATCGAA GAGATCTGCC TAGTTGATGA GAACCAGTTC
    ACTCTAACCA TTGCAAACCA AGGCACACCA CTGACCTTCA TGCACCAAGA ATGTGAAGCC
    ATTGTTCAGT CTATCATTCA TATCAGGACC CGCTGGGAGC TGTCACAACC TGACTCCATC
    CCTCAGCATA CCAAGATTCG ACCAAAGGAT GTCCCTGGGA CACTGCTTAA TATTGCGTTA
    CTGAATTTGG GCAGTTCAGA CCCTAGTTTA CGGTCTGCTG CCTATAATCT CCTGTGTGCC
    TTAACCTGCA CCTTTAATTT AAAGATTGAA GGTCAGTTGC TAGAGACATC GGGGTTATGC
    ATCCCTGCCA ACAACACACT CTTCATTGTC TCTATCAGTA AGACGCTCGC AGCCAATGAG
    CCACACCTCA CCTTAGAGTT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAATTGAAGC ACCTTTGTTT GGAATATATG ACCCCATGGC TGTCAAATCT AGTCCGTTTT
    TGTAAGCATA ATGATGATGC CAAACGACAA AGGGTTACTG CCATCCTTGA TAAGCTAATA
    ACAATGACTA TAAACGAGAA GCAGATGTAT CCTTCTATTC AAGCAAAAAT CTGGGGGAGC
    CTTGGGCAGA TCACAGACCT GCTGGATGTT GTGCTTGACA GTTTCATCAA GACCAGTGCG
    ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG GTGATGGCAG ACACAGCTGT GGCTTTAGCT
    TCTGGAAATG TGAAATTGGT GTCGAGTAAG GTTATTGGAA GGATGTGTAA AATAATTGAC
    AAGACTTGCT TATCCCCAAC TCCAACTTTA GAACAACATC TTATGTGGGA CGACATTGCC
    ATTTTAGCCC GCTACATGCT GATGCTGTCC TTCAACAACT CCCTCGATGT GGCGGCTCAT
    CTGCCCTATC TCTTCCATGT TGTCACTTTC TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA
    GCTTCCACAC ATGGGCTGCT CATCAATATC ATTCACTCTC TGTGTACTTG TTCTCAGCTT
    CACTTTAGTG AAGAGACCAA GCAAGTTTTG AGGCTCAGTC TAACAGAGTT CTCGTTACCC
    AAATTTTACT TACTGTTTGG CATTAGCAAA GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT
    TCCAGTTACC GGGACCGCTC CTTCTCCCCT GGCTCCTATG AGAGGGAGAC TTTTGCTTTG
    ACGTCCCTGG AAACAGTCAC AGAAGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT
    ATTCCAACAT GCAAGTGGCT GGATCAGTGG ACAGAACTAG CTCAAAGATT TGCGTTTCAG
    TATAACCCAT CGCTGCAGCC AAGAGCTCTT GTGGTGTTTG GCTGTATTAG CAAACGAGTG
    TCTCATGGGC AGATAAAGCA GATTATCCGA ATTCTTAGCA AGGCACTTGA AAGTTGTTTA
    AAAGGACCTG ACACTTACAA CAGTCAAGTT CTGATAGAAT CTACGGTGAT AGCACTAACA
    AAATTACAGC CGCTTCTTAA TAAGGACTCG CCCCTGCACA AAGCCCTCTT TTGGGTTGCT
    GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC TTGTATTCAG CCGGCACTGC ACTTCTGGAA
    CAAAACCTGC ACACCTTGGA CAGTCTCCGG ATATTCAATG ACAAGAGTCC GGAAGAAGTA
    TTTATGGCGA TCCGGAATCC TCTGGAATGG CACTGCAAGC AGATGGATCA CTTTGTCGGA
    CTCAACTTCA ACTCTAACTT TAACTTTGCA CTAGTTGGAC ACCTCTTAAA AGGGTACAGG
    CATCCTTCAC CTGCCATTGT TGCAAGAACA GTCAGAATTT TGCATACACT ACTAACTCTC
    GTTAACAAAC ATAGAAATTG TGACAAATTT GAGGTGAATA CACAGAGTGT GGCTTACTTA
    GCAGCTCTAC TCACAGTGTC TGAAGAGGTT CGAAGTCGCT GTAGCCTCAA GCATAGGAAG
    TCCCTTCTTC TTACTGATAT TTCAATGGAA AATGTTCCTA TGGATACATA TCCCATTCAT
    CACGGTGACC CCAGCTATAG GACACTAAAG GAGACCCAGC CATGGTCCTC TCCCAAAGGT
    TCAGAAGGAT ACCTTGCAGC CACCTACCCA GCTGTAGGCC AAACCAGTCC CCGAGCCAGG
    AAATCCATGA GCTTGGATAT GGGACAGCCT TCTCAGGCCA ACACAAAAAA ATTGCTTGGA
    ACAAGAAAAA GTTTTGATCA CTTGATATCT GACACAAAGG CTCCTAAAAG ACAAGAAATG
    GAATCAGGGA TTACAACACC CCCGAAAATG AGAAGAGTGG CAGAGACTGA CTATGAAATG
    GAAACCCAAA GGATTCCCTC CTCGCAGCAG CACCCACATT TACGGAAAGT CTCAGTCTCT
    GAATCAAATG TTCTTTTGGA TGAAGAAGTT CTTACCGACC CAAAGATCCA AGCGCTGCTT
    CTTACTGTTC TGGCTACACT GGTAAAATAT ACTACAGATG AATTTGATCA ACGGATTCTT
    TATGAATATC TAGCAGAGGC CAGCGTTGTG TTTCCCAAAG TGTTTCCTGT TGTGCACAAT
    TTGTTGGACT CTAAGATCAA TACCCTCTTG TCTTTATGCC AAGATCCAAA TTTGTTAAAT
    CCAATCCATG GAATTGTACA GAGTGTGGTG TACCATGAGG AGTCCCCGCC ACAGTACCAG
    ACATCTTACC TGCAAAGTTT TGGTTTTAAT GGCTTATGGC GGTTTGCAGG ACCCTTTTCA
    AAGCAAACAC AAATCCCTGA CTATGCTGAG CTCATTGTTA AATTTCTTGA TGCCTTGATT
    GACACGTACT TGCCTGGAAT TGATGAAGAA ACCAGTGAGG AGTCCCTCCT GACACCCACA
    TCTCCTTACC CTCCTGCACT GCAGAGCCAG CTTAGTATCA CTGCCAACCT TAACCTCTCT
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GACTCGACAA GGAGAACGTT
    GAACTCTCCC CCACCGCTGG CCACTGTAAC AGTGGACGGA CTCGCCATGG ATCCGCAAGC
    CAAGTGCAGA AGCAAAGAAG TGCTGGCAGT TTCAAACGTA ATAGCATTAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45708
    Clone ID Related Accession (Same CDS sequence) XM_006532442.4 , XM_006532442.3
    Accession Version XM_006532442.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532442.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAG AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGCTTCCCT CAGAACAGCA TCGGTGCCGT AGGAAGTGCC
    ATGTTTCTCA GATTCATCAA TCCTGCCATT GTCTCGCCGT ATGAAGCAGG GATTTTAGAT
    AAAAAGCCAC CACCTAGAAT TGAAAGGGGC TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC
    ATTGCCAATC ATGTACTGTT CACAAAAGAA GAGCATATGA GACCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGACTTGGC ACGAAGGTTT TTCCTAGATA TAGCATCAGA TTGTCCCACA
    AGTGATGCAG TAAACCATAG TCTTTCCTTC ATCAGCGATG GCAATGTGCT TGCTTTACAT
    CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGTAA CAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCACAC TTCTCGCGTA TCTGGGTCCT
    CCGGAGCACA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTTGAGGAAT TTATGACCAG GCACCAGGTA CATGAGAAAG AAGAGTTCAA GGCCTTGAAA
    ACGTTAAGCA TCTTCTACCA AGCTGGCACC TCCAAAGCTG GGAATCCTAT TTTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGCCAGATC AATGGTGATC TGCTGATATA CCATGTCTTG
    CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT
    GGGCCTAGCA ATCGCTTTAA AACAGACTTC CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC
    TTTGCCTATG ACAACGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TATACCAAAT ACCATGAGCG GCTGCTGACT GGCCTCAAGG GCAGCAAAAG GCTCATTTTC
    ATCGACTGTC CTGGGAAGCT GGCTGAACAT ATAGAGCATG AGCAACAGAA ACTCCCTGCT
    GCTACCTTGG CTTTGGAAGA AGATCTAAAG GTTTTCCACA ATGCTCTCAA GTTAGCTCAC
    AAGGACACCA AAGTTTCTAT TAAGGTTGGT TCTACTGCTG TTCAGGTAAC TTCAGCAGAA
    AGAACAAAAG TTTTGGGGCA ATCCGTCTTT TTAAATGATA TTTACTATGC TTCTGAAATC
    GAAGAGATCT GCCTAGTTGA TGAGAACCAG TTCACTCTAA CCATTGCAAA CCAAGGCACA
    CCACTGACCT TCATGCACCA AGAATGTGAA GCCATTGTTC AGTCTATCAT TCATATCAGG
    ACCCGCTGGG AGCTGTCACA ACCTGACTCC ATCCCTCAGC ATACCAAGAT TCGACCAAAG
    GATGTCCCTG GGACACTGCT TAATATTGCG TTACTGAATT TGGGCAGTTC AGACCCTAGT
    TTACGGTCTG CTGCCTATAA TCTCCTGTGT GCCTTAACCT GCACCTTTAA TTTAAAGATT
    GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA TGCATCCCTG CCAACAACAC ACTCTTCATT
    GTCTCTATCA GTAAGACGCT CGCAGCCAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT
    ATGACCCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ATAATGATGA TGCCAAACGA
    CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CTATAAACGA GAAGCAGATG
    TATCCTTCTA TTCAAGCAAA AATCTGGGGG AGCCTTGGGC AGATCACAGA CCTGCTGGAT
    GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCGACAGGAG GCTTAGGGTC TATCAAAGCT
    GAGGTGATGG CAGACACAGC TGTGGCTTTA GCTTCTGGAA ATGTGAAATT GGTGTCGAGT
    AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCCCC AACTCCAACT
    TTAGAACAAC ATCTTATGTG GGACGACATT GCCATTTTAG CCCGCTACAT GCTGATGCTG
    TCCTTCAACA ACTCCCTCGA TGTGGCGGCT CATCTGCCCT ATCTCTTCCA TGTTGTCACT
    TTCTTAGTAG CCACAGGTCC CTTGTCCCTC CGAGCTTCCA CACATGGGCT GCTCATCAAT
    ATCATTCACT CTCTGTGTAC TTGTTCTCAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT
    TTGAGGCTCA GTCTAACAGA GTTCTCGTTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CGGCTGCTGT CATTGCCTTC CGTTCCAGTT ACCGGGACCG CTCCTTCTCC
    CCTGGCTCCT ATGAGAGGGA GACTTTTGCT TTGACGTCCC TGGAAACAGT CACAGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG ATTTGCGTTT CAGTATAACC CATCGCTGCA GCCAAGAGCT
    CTTGTGGTGT TTGGCTGTAT TAGCAAACGA GTGTCTCATG GGCAGATAAA GCAGATTATC
    CGAATTCTTA GCAAGGCACT TGAAAGTTGT TTAAAAGGAC CTGACACTTA CAACAGTCAA
    GTTCTGATAG AATCTACGGT GATAGCACTA ACAAAATTAC AGCCGCTTCT TAATAAGGAC
    TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT GCTGTGGCTG TGCTGCAGCT GGACGAAGTC
    AACTTGTATT CAGCCGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT GGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCGGAAGAA GTATTTATGG CGATCCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAGATGGA TCACTTTGTC GGACTCAACT TCAACTCTAA CTTTAACTTT
    GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTCGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG
    GTTCGAAGTC GCTGTAGCCT CAAGCATAGG AAGTCCCTTC TTCTTACTGA TATTTCAATG
    GAAAATGTTC CTATGGATAC ATATCCCATT CATCACGGTG ACCCCAGCTA TAGGACACTA
    AAGGAGACCC AGCCATGGTC CTCTCCCAAA GGTTCAGAAG GATACCTTGC AGCCACCTAC
    CCAGCTGTAG GCCAAACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA TATGGGACAG
    CCTTCTCAGG CCAACACAAA AAAATTGCTT GGAACAAGAA AAAGTTTTGA TCACTTGATA
    TCTGACACAA AGGCTCCTAA AAGACAAGAA ATGGAATCAG GGATTACAAC ACCCCCGAAA
    ATGAGAAGAG TGGCAGAGAC TGACTATGAA ATGGCAGAAA CCCAAAGGAT TCCCTCCTCG
    CAGCAGCACC CACATTTACG GAAAGTCTCA GTCTCTGAAT CAAATGTTCT TTTGGATGAA
    GAAGTTCTTA CCGACCCAAA GATCCAAGCG CTGCTTCTTA CTGTTCTGGC TACACTGGTA
    AAATATACTA CAGATGAATT TGATCAACGG ATTCTTTATG AATATCTAGC AGAGGCCAGC
    GTTGTGTTTC CCAAAGTGTT TCCTGTTGTG CACAATTTGT TGGACTCTAA GATCAATACC
    CTCTTGTCTT TATGCCAAGA TCCAAATTTG TTAAATCCAA TCCATGGAAT TGTACAGAGT
    GTGGTGTACC ATGAGGAGTC CCCGCCACAG TACCAGACAT CTTACCTGCA AAGTTTTGGT
    TTTAATGGCT TATGGCGGTT TGCAGGACCC TTTTCAAAGG ACTCGACAAG GAGAACGTTG
    AACTCTCCCC CACCGCTGGC CACTGTAACA GTGGACGGAC TCGCCATGGA TCCGCAAGCC
    AAGTGCAGAA GCAAAGAAGT GCTGGCAGTT TCAAACGTAA TAGCATTAAG AAGATCGTGT
    GAAGCGTTCT TTGTTTCACT CTTCAAACCA ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532505.1
    CDS411..8786
    Translation

    Target ORF information:

    RefSeq Version XM_006532442.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromin 1 (Nf1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532442.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAG AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGCTTCCCT CAGAACAGCA TCGGTGCCGT AGGAAGTGCC
    ATGTTTCTCA GATTCATCAA TCCTGCCATT GTCTCGCCGT ATGAAGCAGG GATTTTAGAT
    AAAAAGCCAC CACCTAGAAT TGAAAGGGGC TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC
    ATTGCCAATC ATGTACTGTT CACAAAAGAA GAGCATATGA GACCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGACTTGGC ACGAAGGTTT TTCCTAGATA TAGCATCAGA TTGTCCCACA
    AGTGATGCAG TAAACCATAG TCTTTCCTTC ATCAGCGATG GCAATGTGCT TGCTTTACAT
    CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGTAA CAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCACAC TTCTCGCGTA TCTGGGTCCT
    CCGGAGCACA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTTGAGGAAT TTATGACCAG GCACCAGGTA CATGAGAAAG AAGAGTTCAA GGCCTTGAAA
    ACGTTAAGCA TCTTCTACCA AGCTGGCACC TCCAAAGCTG GGAATCCTAT TTTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGCCAGATC AATGGTGATC TGCTGATATA CCATGTCTTG
    CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT
    GGGCCTAGCA ATCGCTTTAA AACAGACTTC CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC
    TTTGCCTATG ACAACGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TATACCAAAT ACCATGAGCG GCTGCTGACT GGCCTCAAGG GCAGCAAAAG GCTCATTTTC
    ATCGACTGTC CTGGGAAGCT GGCTGAACAT ATAGAGCATG AGCAACAGAA ACTCCCTGCT
    GCTACCTTGG CTTTGGAAGA AGATCTAAAG GTTTTCCACA ATGCTCTCAA GTTAGCTCAC
    AAGGACACCA AAGTTTCTAT TAAGGTTGGT TCTACTGCTG TTCAGGTAAC TTCAGCAGAA
    AGAACAAAAG TTTTGGGGCA ATCCGTCTTT TTAAATGATA TTTACTATGC TTCTGAAATC
    GAAGAGATCT GCCTAGTTGA TGAGAACCAG TTCACTCTAA CCATTGCAAA CCAAGGCACA
    CCACTGACCT TCATGCACCA AGAATGTGAA GCCATTGTTC AGTCTATCAT TCATATCAGG
    ACCCGCTGGG AGCTGTCACA ACCTGACTCC ATCCCTCAGC ATACCAAGAT TCGACCAAAG
    GATGTCCCTG GGACACTGCT TAATATTGCG TTACTGAATT TGGGCAGTTC AGACCCTAGT
    TTACGGTCTG CTGCCTATAA TCTCCTGTGT GCCTTAACCT GCACCTTTAA TTTAAAGATT
    GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA TGCATCCCTG CCAACAACAC ACTCTTCATT
    GTCTCTATCA GTAAGACGCT CGCAGCCAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT
    ATGACCCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ATAATGATGA TGCCAAACGA
    CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CTATAAACGA GAAGCAGATG
    TATCCTTCTA TTCAAGCAAA AATCTGGGGG AGCCTTGGGC AGATCACAGA CCTGCTGGAT
    GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCGACAGGAG GCTTAGGGTC TATCAAAGCT
    GAGGTGATGG CAGACACAGC TGTGGCTTTA GCTTCTGGAA ATGTGAAATT GGTGTCGAGT
    AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCCCC AACTCCAACT
    TTAGAACAAC ATCTTATGTG GGACGACATT GCCATTTTAG CCCGCTACAT GCTGATGCTG
    TCCTTCAACA ACTCCCTCGA TGTGGCGGCT CATCTGCCCT ATCTCTTCCA TGTTGTCACT
    TTCTTAGTAG CCACAGGTCC CTTGTCCCTC CGAGCTTCCA CACATGGGCT GCTCATCAAT
    ATCATTCACT CTCTGTGTAC TTGTTCTCAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT
    TTGAGGCTCA GTCTAACAGA GTTCTCGTTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CGGCTGCTGT CATTGCCTTC CGTTCCAGTT ACCGGGACCG CTCCTTCTCC
    CCTGGCTCCT ATGAGAGGGA GACTTTTGCT TTGACGTCCC TGGAAACAGT CACAGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG ATTTGCGTTT CAGTATAACC CATCGCTGCA GCCAAGAGCT
    CTTGTGGTGT TTGGCTGTAT TAGCAAACGA GTGTCTCATG GGCAGATAAA GCAGATTATC
    CGAATTCTTA GCAAGGCACT TGAAAGTTGT TTAAAAGGAC CTGACACTTA CAACAGTCAA
    GTTCTGATAG AATCTACGGT GATAGCACTA ACAAAATTAC AGCCGCTTCT TAATAAGGAC
    TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT GCTGTGGCTG TGCTGCAGCT GGACGAAGTC
    AACTTGTATT CAGCCGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT GGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCGGAAGAA GTATTTATGG CGATCCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAGATGGA TCACTTTGTC GGACTCAACT TCAACTCTAA CTTTAACTTT
    GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTCGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG
    GTTCGAAGTC GCTGTAGCCT CAAGCATAGG AAGTCCCTTC TTCTTACTGA TATTTCAATG
    GAAAATGTTC CTATGGATAC ATATCCCATT CATCACGGTG ACCCCAGCTA TAGGACACTA
    AAGGAGACCC AGCCATGGTC CTCTCCCAAA GGTTCAGAAG GATACCTTGC AGCCACCTAC
    CCAGCTGTAG GCCAAACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA TATGGGACAG
    CCTTCTCAGG CCAACACAAA AAAATTGCTT GGAACAAGAA AAAGTTTTGA TCACTTGATA
    TCTGACACAA AGGCTCCTAA AAGACAAGAA ATGGAATCAG GGATTACAAC ACCCCCGAAA
    ATGAGAAGAG TGGCAGAGAC TGACTATGAA ATGGCAGAAA CCCAAAGGAT TCCCTCCTCG
    CAGCAGCACC CACATTTACG GAAAGTCTCA GTCTCTGAAT CAAATGTTCT TTTGGATGAA
    GAAGTTCTTA CCGACCCAAA GATCCAAGCG CTGCTTCTTA CTGTTCTGGC TACACTGGTA
    AAATATACTA CAGATGAATT TGATCAACGG ATTCTTTATG AATATCTAGC AGAGGCCAGC
    GTTGTGTTTC CCAAAGTGTT TCCTGTTGTG CACAATTTGT TGGACTCTAA GATCAATACC
    CTCTTGTCTT TATGCCAAGA TCCAAATTTG TTAAATCCAA TCCATGGAAT TGTACAGAGT
    GTGGTGTACC ATGAGGAGTC CCCGCCACAG TACCAGACAT CTTACCTGCA AAGTTTTGGT
    TTTAATGGCT TATGGCGGTT TGCAGGACCC TTTTCAAAGG ACTCGACAAG GAGAACGTTG
    AACTCTCCCC CACCGCTGGC CACTGTAACA GTGGACGGAC TCGCCATGGA TCCGCAAGCC
    AAGTGCAGAA GCAAAGAAGT GCTGGCAGTT TCAAACGTAA TAGCATTAAG AAGATCGTGT
    GAAGCGTTCT TTGTTTCACT CTTCAAACCA ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45708
    Clone ID Related Accession (Same CDS sequence) XM_006532442.4 , XM_006532442.3
    Accession Version XM_006532442.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2221
    2281
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    2521
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    2641
    2701
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    3001
    3061
    3121
    3181
    3241
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    3421
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    4141
    4201
    4261
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    4381
    4441
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    4681
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    5641
    5701
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6721
    6781
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    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAG AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGCTTCCCT CAGAACAGCA TCGGTGCCGT AGGAAGTGCC
    ATGTTTCTCA GATTCATCAA TCCTGCCATT GTCTCGCCGT ATGAAGCAGG GATTTTAGAT
    AAAAAGCCAC CACCTAGAAT TGAAAGGGGC TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC
    ATTGCCAATC ATGTACTGTT CACAAAAGAA GAGCATATGA GACCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGACTTGGC ACGAAGGTTT TTCCTAGATA TAGCATCAGA TTGTCCCACA
    AGTGATGCAG TAAACCATAG TCTTTCCTTC ATCAGCGATG GCAATGTGCT TGCTTTACAT
    CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGTAA CAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCACAC TTCTCGCGTA TCTGGGTCCT
    CCGGAGCACA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTTGAGGAAT TTATGACCAG GCACCAGGTA CATGAGAAAG AAGAGTTCAA GGCCTTGAAA
    ACGTTAAGCA TCTTCTACCA AGCTGGCACC TCCAAAGCTG GGAATCCTAT TTTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGCCAGATC AATGGTGATC TGCTGATATA CCATGTCTTG
    CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT
    GGGCCTAGCA ATCGCTTTAA AACAGACTTC CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC
    TTTGCCTATG ACAACGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TATACCAAAT ACCATGAGCG GCTGCTGACT GGCCTCAAGG GCAGCAAAAG GCTCATTTTC
    ATCGACTGTC CTGGGAAGCT GGCTGAACAT ATAGAGCATG AGCAACAGAA ACTCCCTGCT
    GCTACCTTGG CTTTGGAAGA AGATCTAAAG GTTTTCCACA ATGCTCTCAA GTTAGCTCAC
    AAGGACACCA AAGTTTCTAT TAAGGTTGGT TCTACTGCTG TTCAGGTAAC TTCAGCAGAA
    AGAACAAAAG TTTTGGGGCA ATCCGTCTTT TTAAATGATA TTTACTATGC TTCTGAAATC
    GAAGAGATCT GCCTAGTTGA TGAGAACCAG TTCACTCTAA CCATTGCAAA CCAAGGCACA
    CCACTGACCT TCATGCACCA AGAATGTGAA GCCATTGTTC AGTCTATCAT TCATATCAGG
    ACCCGCTGGG AGCTGTCACA ACCTGACTCC ATCCCTCAGC ATACCAAGAT TCGACCAAAG
    GATGTCCCTG GGACACTGCT TAATATTGCG TTACTGAATT TGGGCAGTTC AGACCCTAGT
    TTACGGTCTG CTGCCTATAA TCTCCTGTGT GCCTTAACCT GCACCTTTAA TTTAAAGATT
    GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA TGCATCCCTG CCAACAACAC ACTCTTCATT
    GTCTCTATCA GTAAGACGCT CGCAGCCAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT
    ATGACCCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ATAATGATGA TGCCAAACGA
    CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CTATAAACGA GAAGCAGATG
    TATCCTTCTA TTCAAGCAAA AATCTGGGGG AGCCTTGGGC AGATCACAGA CCTGCTGGAT
    GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCGACAGGAG GCTTAGGGTC TATCAAAGCT
    GAGGTGATGG CAGACACAGC TGTGGCTTTA GCTTCTGGAA ATGTGAAATT GGTGTCGAGT
    AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCCCC AACTCCAACT
    TTAGAACAAC ATCTTATGTG GGACGACATT GCCATTTTAG CCCGCTACAT GCTGATGCTG
    TCCTTCAACA ACTCCCTCGA TGTGGCGGCT CATCTGCCCT ATCTCTTCCA TGTTGTCACT
    TTCTTAGTAG CCACAGGTCC CTTGTCCCTC CGAGCTTCCA CACATGGGCT GCTCATCAAT
    ATCATTCACT CTCTGTGTAC TTGTTCTCAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT
    TTGAGGCTCA GTCTAACAGA GTTCTCGTTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CGGCTGCTGT CATTGCCTTC CGTTCCAGTT ACCGGGACCG CTCCTTCTCC
    CCTGGCTCCT ATGAGAGGGA GACTTTTGCT TTGACGTCCC TGGAAACAGT CACAGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG ATTTGCGTTT CAGTATAACC CATCGCTGCA GCCAAGAGCT
    CTTGTGGTGT TTGGCTGTAT TAGCAAACGA GTGTCTCATG GGCAGATAAA GCAGATTATC
    CGAATTCTTA GCAAGGCACT TGAAAGTTGT TTAAAAGGAC CTGACACTTA CAACAGTCAA
    GTTCTGATAG AATCTACGGT GATAGCACTA ACAAAATTAC AGCCGCTTCT TAATAAGGAC
    TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT GCTGTGGCTG TGCTGCAGCT GGACGAAGTC
    AACTTGTATT CAGCCGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT GGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCGGAAGAA GTATTTATGG CGATCCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAGATGGA TCACTTTGTC GGACTCAACT TCAACTCTAA CTTTAACTTT
    GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTCGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG
    GTTCGAAGTC GCTGTAGCCT CAAGCATAGG AAGTCCCTTC TTCTTACTGA TATTTCAATG
    GAAAATGTTC CTATGGATAC ATATCCCATT CATCACGGTG ACCCCAGCTA TAGGACACTA
    AAGGAGACCC AGCCATGGTC CTCTCCCAAA GGTTCAGAAG GATACCTTGC AGCCACCTAC
    CCAGCTGTAG GCCAAACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA TATGGGACAG
    CCTTCTCAGG CCAACACAAA AAAATTGCTT GGAACAAGAA AAAGTTTTGA TCACTTGATA
    TCTGACACAA AGGCTCCTAA AAGACAAGAA ATGGAATCAG GGATTACAAC ACCCCCGAAA
    ATGAGAAGAG TGGCAGAGAC TGACTATGAA ATGGCAGAAA CCCAAAGGAT TCCCTCCTCG
    CAGCAGCACC CACATTTACG GAAAGTCTCA GTCTCTGAAT CAAATGTTCT TTTGGATGAA
    GAAGTTCTTA CCGACCCAAA GATCCAAGCG CTGCTTCTTA CTGTTCTGGC TACACTGGTA
    AAATATACTA CAGATGAATT TGATCAACGG ATTCTTTATG AATATCTAGC AGAGGCCAGC
    GTTGTGTTTC CCAAAGTGTT TCCTGTTGTG CACAATTTGT TGGACTCTAA GATCAATACC
    CTCTTGTCTT TATGCCAAGA TCCAAATTTG TTAAATCCAA TCCATGGAAT TGTACAGAGT
    GTGGTGTACC ATGAGGAGTC CCCGCCACAG TACCAGACAT CTTACCTGCA AAGTTTTGGT
    TTTAATGGCT TATGGCGGTT TGCAGGACCC TTTTCAAAGG ACTCGACAAG GAGAACGTTG
    AACTCTCCCC CACCGCTGGC CACTGTAACA GTGGACGGAC TCGCCATGGA TCCGCAAGCC
    AAGTGCAGAA GCAAAGAAGT GCTGGCAGTT TCAAACGTAA TAGCATTAAG AAGATCGTGT
    GAAGCGTTCT TTGTTTCACT CTTCAAACCA ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532505.1
    CDS1612..9987
    Translation

    Target ORF information:

    RefSeq Version XM_006532442.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromatosis 1 (Nf1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532442.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCGTTGG ATTGGTGGCC TAAGATTGAT
    GCTGTATATT GTCACTCAGT TGAACTTCGC AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGCT GTGGAGCGCA CCCAGCAATA CGAATGGCAC CAAGTCTCAC CTTTAAAGAA
    AAGGTAACAA GCCTTAAATT TAAAGAAAAG CCTACAGACC TAGAGACAAG GAGCTACAAA
    TGTCTTCTTT TGTCCATGGT GAAACTGATT CATGCAGACC CAAAGCTTTT GCTTTGTAAT
    CCAAGAAAAC AGGGCCCTGA AACTCAGAGC AGTACAGCAG AACTAATTAC AGGGCTCGTA
    CAACTGGTTC CTCAGTCACA CATGCCAGAG GTCGCTCAGG AGGCTATGGA GGCTTTGCTG
    GTCCTTCATC AGTTAGATAG CATTGATCTC TGGAATCCTG ACGCTCCTGT AGAGACATTC
    TGGGAAATCA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG CCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTA CGGGAAATTC TGATCTGCAG AAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGC TCCTGTCATT CTCTCTACCT TTATGGAGTA
    GGATGTGAAA TGTCTGCTAC TGGAAATACC ACTCAGATGT CAGTTGATCA TGATGAGTTC
    CTGCGTGCCT GTACTCCTGG AGCATCTCTG CGGAAAGGAA GAGGGAATTC CTCCATGGAT
    AGCACAGCAG GGTGCAGTGG AACCCCACCT ATATGCCGAC AAGCCCAGAC CAAGCTAGAA
    GTGGCCTTAT ACATGTTTCT GTGGAATCCT GACACTGAAG CTGTTCTGGT TGCTATGTCC
    TGTTTCCGCC ACCTCTGTGA AGAAGCAGAT ATTCGATGTG GGGTAGATGA AGTGTCAGTA
    CACAACTTCT TGCCCAACTA TAACACATTC ATGGAATTTG CCTCAGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCGCT TCAGAAAAGA GTGATGGCCC TGCTAAGGCG CATTGAGCAC
    CCTACTGCAG GAAACATTGA GGCCTGGGAA GATACACATG CAAAGTGGGA ACAGGCTACA
    AAACTAATCC TTAACTACCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC AGAAAGTCTT
    CATAAGACCA TTGTTAAGAG ACGGATGTCC CATGTCAGTG GAGGAGGCTC CATAGACTTG
    TCTGACACAG ACTCCCTGCA GGAGTGGATC AACATGACTG GCTTCCTTTG TGCCCTTGGT
    GGGGTGTGCC TGCAACAGAG AAGCAGCTCT GGCCTAGCGA CATACAGCCC ACCTATGGGC
    GCTGTCAGTG AACGCAAAGG GTCTATGATT TCTGTAATGT CTTCTGAAGG GAATATTGAT
    TCACCTGTCA GCAGATTTAT GGACCGGCTT CTGTCCTTAA TGGTGTGTAA CCACGAGAAA
    GTGGGGCTTC AGATACGGAC CAATGTTAAG GACCTGGTGG GTCTGGAGTT GAGTCCTGCT
    CTGTATCCAA TGCTGTTTAA CAAACTGAAG AATACCATCA GCAAGTTTTT TGACTCTCAA
    GGACAGGTAT TACTAAGTGA CAGCAATACT CAGTTTGTAG AGCAAACCAT AGCCATAATG
    AAAAACCTAC TGGATAATCA TACTGAAGGC AGCTCTGAGC ATCTGGGACA AGCGAGCATT
    GAGACAATGA TGCTAAATCT GGTCAGATAC GTTCGTGTTC TTGGGAATAT GGTCCATGCA
    ATACAAATAA AAACAAAATT GTGTCAGTTA GTTGAAGTCA TGATGGCAAG AAGAGATGAC
    CTCTCGTTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT AACAGACTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGACATAA AATGTCTTAC GAGAGATCTG
    GACCAAGCAA GCATGGAAGC AGTAGTTTCT CTCCTCGCTG GTCTTCCACT GCAGCCCGAA
    GAAGGAGATG GGGTGGAGCT GATGGAGGCC AAATCACAGT TATTCCTTAA GTACTTCACA
    TTATTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACGGGT
    GGCAGGAAAC GTGGCATGTC TCGGAGGCTG GCATCCCTGA GGCACTGTAC AGTGCTTGCA
    ATGTCAAACT TACTCAATGC TAATGTGGAC AGTGGACTCA TGCACTCCAT AGGCTTAGGT
    TATCACAAGG ATCTTCAGAC AAGAGCTACG TTTATGGAAG TTCTGACAAA AATCCTTCAA
    CAAGGAACAG AATTCGATAC ACTTGCGGAA ACGGTGTTGG CAGATCGATT TGAGAGACTT
    GTGGAACTGG TCACAATGAT GGGAGACCAG GGAGAACTCC CTATAGCTAT GGCTCTTGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAG CTGGCTCGGG TTCTGGTCAC TCTGTTTGAT
    TCCCGGCATT TACTCTACCA GCTGCTCTGG AACATGTTTT CTAAGGAGGT AGAATTGGCA
    GACTCCATGC AGACTCTCTT TCGAGGCAAC AGCTTGGCCA GTAAGATAAT GACATTCTGC
    TTCAAGGTGT ATGGTGCTAC TTACCTACAA AAGCTCTTGG ACCCTTTATT ACGAGTCATC
    ATCACATCTT CGGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACCC
    TCTGAGAGCC TTGAGGAGAA CCAGAGGAAC CTCCTTCAGA TGACAGAGAA GTTCTTCCAT
    GCCATCATCA GCTCTTCCTC AGAATTCCCC TCACAGCTTC GAAGTGTCTG CCATTGTTTG
    TACCAGGCAA CTTGCCACTC CCTACTGAAT AAAGCTACAG TAAAAGAAAG AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGCTTCCCT CAGAACAGCA TCGGTGCCGT AGGAAGTGCC
    ATGTTTCTCA GATTCATCAA TCCTGCCATT GTCTCGCCGT ATGAAGCAGG GATTTTAGAT
    AAAAAGCCAC CACCTAGAAT TGAAAGGGGC TTGAAGTTAA TGTCAAAGGT ACTTCAGAGC
    ATTGCCAATC ATGTACTGTT CACAAAAGAA GAGCATATGA GACCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGACTTGGC ACGAAGGTTT TTCCTAGATA TAGCATCAGA TTGTCCCACA
    AGTGATGCAG TAAACCATAG TCTTTCCTTC ATCAGCGATG GCAATGTGCT TGCTTTACAT
    CGGCTGCTTT GGAACAATCA GGAGAAAATT GGCCAGTATC TTTCCAGTAA CAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCCACAC TTCTCGCGTA TCTGGGTCCT
    CCGGAGCACA AGCCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTTGAGGAAT TTATGACCAG GCACCAGGTA CATGAGAAAG AAGAGTTCAA GGCCTTGAAA
    ACGTTAAGCA TCTTCTACCA AGCTGGCACC TCCAAAGCTG GGAATCCTAT TTTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGCCAGATC AATGGTGATC TGCTGATATA CCATGTCTTG
    CTGACTTTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACT
    GGGCCTAGCA ATCGCTTTAA AACAGACTTC CTCTCTAAGT GGTTTGTTGT CTTTCCTGGC
    TTTGCCTATG ACAACGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TATACCAAAT ACCATGAGCG GCTGCTGACT GGCCTCAAGG GCAGCAAAAG GCTCATTTTC
    ATCGACTGTC CTGGGAAGCT GGCTGAACAT ATAGAGCATG AGCAACAGAA ACTCCCTGCT
    GCTACCTTGG CTTTGGAAGA AGATCTAAAG GTTTTCCACA ATGCTCTCAA GTTAGCTCAC
    AAGGACACCA AAGTTTCTAT TAAGGTTGGT TCTACTGCTG TTCAGGTAAC TTCAGCAGAA
    AGAACAAAAG TTTTGGGGCA ATCCGTCTTT TTAAATGATA TTTACTATGC TTCTGAAATC
    GAAGAGATCT GCCTAGTTGA TGAGAACCAG TTCACTCTAA CCATTGCAAA CCAAGGCACA
    CCACTGACCT TCATGCACCA AGAATGTGAA GCCATTGTTC AGTCTATCAT TCATATCAGG
    ACCCGCTGGG AGCTGTCACA ACCTGACTCC ATCCCTCAGC ATACCAAGAT TCGACCAAAG
    GATGTCCCTG GGACACTGCT TAATATTGCG TTACTGAATT TGGGCAGTTC AGACCCTAGT
    TTACGGTCTG CTGCCTATAA TCTCCTGTGT GCCTTAACCT GCACCTTTAA TTTAAAGATT
    GAAGGTCAGT TGCTAGAGAC ATCGGGGTTA TGCATCCCTG CCAACAACAC ACTCTTCATT
    GTCTCTATCA GTAAGACGCT CGCAGCCAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAATTGA AGCACCTTTG TTTGGAATAT
    ATGACCCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ATAATGATGA TGCCAAACGA
    CAAAGGGTTA CTGCCATCCT TGATAAGCTA ATAACAATGA CTATAAACGA GAAGCAGATG
    TATCCTTCTA TTCAAGCAAA AATCTGGGGG AGCCTTGGGC AGATCACAGA CCTGCTGGAT
    GTTGTGCTTG ACAGTTTCAT CAAGACCAGT GCGACAGGAG GCTTAGGGTC TATCAAAGCT
    GAGGTGATGG CAGACACAGC TGTGGCTTTA GCTTCTGGAA ATGTGAAATT GGTGTCGAGT
    AAGGTTATTG GAAGGATGTG TAAAATAATT GACAAGACTT GCTTATCCCC AACTCCAACT
    TTAGAACAAC ATCTTATGTG GGACGACATT GCCATTTTAG CCCGCTACAT GCTGATGCTG
    TCCTTCAACA ACTCCCTCGA TGTGGCGGCT CATCTGCCCT ATCTCTTCCA TGTTGTCACT
    TTCTTAGTAG CCACAGGTCC CTTGTCCCTC CGAGCTTCCA CACATGGGCT GCTCATCAAT
    ATCATTCACT CTCTGTGTAC TTGTTCTCAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT
    TTGAGGCTCA GTCTAACAGA GTTCTCGTTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CGGCTGCTGT CATTGCCTTC CGTTCCAGTT ACCGGGACCG CTCCTTCTCC
    CCTGGCTCCT ATGAGAGGGA GACTTTTGCT TTGACGTCCC TGGAAACAGT CACAGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG ATTTGCGTTT CAGTATAACC CATCGCTGCA GCCAAGAGCT
    CTTGTGGTGT TTGGCTGTAT TAGCAAACGA GTGTCTCATG GGCAGATAAA GCAGATTATC
    CGAATTCTTA GCAAGGCACT TGAAAGTTGT TTAAAAGGAC CTGACACTTA CAACAGTCAA
    GTTCTGATAG AATCTACGGT GATAGCACTA ACAAAATTAC AGCCGCTTCT TAATAAGGAC
    TCGCCCCTGC ACAAAGCCCT CTTTTGGGTT GCTGTGGCTG TGCTGCAGCT GGACGAAGTC
    AACTTGTATT CAGCCGGCAC TGCACTTCTG GAACAAAACC TGCACACCTT GGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCGGAAGAA GTATTTATGG CGATCCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAGATGGA TCACTTTGTC GGACTCAACT TCAACTCTAA CTTTAACTTT
    GCACTAGTTG GACACCTCTT AAAAGGGTAC AGGCATCCTT CACCTGCCAT TGTTGCAAGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTCGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACACAGAG TGTGGCTTAC TTAGCAGCTC TACTCACAGT GTCTGAAGAG
    GTTCGAAGTC GCTGTAGCCT CAAGCATAGG AAGTCCCTTC TTCTTACTGA TATTTCAATG
    GAAAATGTTC CTATGGATAC ATATCCCATT CATCACGGTG ACCCCAGCTA TAGGACACTA
    AAGGAGACCC AGCCATGGTC CTCTCCCAAA GGTTCAGAAG GATACCTTGC AGCCACCTAC
    CCAGCTGTAG GCCAAACCAG TCCCCGAGCC AGGAAATCCA TGAGCTTGGA TATGGGACAG
    CCTTCTCAGG CCAACACAAA AAAATTGCTT GGAACAAGAA AAAGTTTTGA TCACTTGATA
    TCTGACACAA AGGCTCCTAA AAGACAAGAA ATGGAATCAG GGATTACAAC ACCCCCGAAA
    ATGAGAAGAG TGGCAGAGAC TGACTATGAA ATGGCAGAAA CCCAAAGGAT TCCCTCCTCG
    CAGCAGCACC CACATTTACG GAAAGTCTCA GTCTCTGAAT CAAATGTTCT TTTGGATGAA
    GAAGTTCTTA CCGACCCAAA GATCCAAGCG CTGCTTCTTA CTGTTCTGGC TACACTGGTA
    AAATATACTA CAGATGAATT TGATCAACGG ATTCTTTATG AATATCTAGC AGAGGCCAGC
    GTTGTGTTTC CCAAAGTGTT TCCTGTTGTG CACAATTTGT TGGACTCTAA GATCAATACC
    CTCTTGTCTT TATGCCAAGA TCCAAATTTG TTAAATCCAA TCCATGGAAT TGTACAGAGT
    GTGGTGTACC ATGAGGAGTC CCCGCCACAG TACCAGACAT CTTACCTGCA AAGTTTTGGT
    TTTAATGGCT TATGGCGGTT TGCAGGACCC TTTTCAAAGG ACTCGACAAG GAGAACGTTG
    AACTCTCCCC CACCGCTGGC CACTGTAACA GTGGACGGAC TCGCCATGGA TCCGCAAGCC
    AAGTGCAGAA GCAAAGAAGT GCTGGCAGTT TCAAACGTAA TAGCATTAAG AAGATCGTGT
    GAAGCGTTCT TTGTTTCACT CTTCAAACCA ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85649
    Clone ID Related Accession (Same CDS sequence) XM_030245644.1
    Accession Version XM_030245644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product neurofibromin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGC
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGCT GTGGAGCGCA CCCAGCAATA
    CGAATGGCAC CAAGTCTCAC CTTTAAAGAA AAGGTAACAA GCCTTAAATT TAAAGAAAAG
    CCTACAGACC TAGAGACAAG GAGCTACAAA TGTCTTCTTT TGTCCATGGT GAAACTGATT
    CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAGAGC
    AGTACAGCAG AACTAATTAC AGGGCTCGTA CAACTGGTTC CTCAGTCACA CATGCCAGAG
    GTCGCTCAGG AGGCTATGGA GGCTTTGCTG GTCCTTCATC AGTTAGATAG CATTGATCTC
    TGGAATCCTG ACGCTCCTGT AGAGACATTC TGGGAAATCA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA
    CGGGAAATTC TGATCTGCAG AAATAAATTT CTTCTTAAAA ATAAGGCAGA TAGAAGCTCC
    TGTCATTCTC TCTACCTTTA TGGAGTAGGA TGTGAAATGT CTGCTACTGG AAATACCACT
    CAGATGTCAG TTGATCATGA TGAGTTCCTG CGTGCCTGTA CTCCTGGAGC ATCTCTGCGG
    AAAGGAAGAG GGAATTCCTC CATGGATAGC ACAGCAGGGT GCAGTGGAAC CCCACCTATA
    TGCCGACAAG CCCAGACCAA GCTAGAAGTG GCCTTATACA TGTTTCTGTG GAATCCTGAC
    ACTGAAGCTG TTCTGGTTGC TATGTCCTGT TTCCGCCACC TCTGTGAAGA AGCAGATATT
    CGATGTGGGG TAGATGAAGT GTCAGTACAC AACTTCTTGC CCAACTATAA CACATTCATG
    GAATTTGCCT CAGTCAGCAA TATGATGTCA ACAGGAAGAG CAGCGCTTCA GAAAAGAGTG
    ATGGCCCTGC TAAGGCGCAT TGAGCACCCT ACTGCAGGAA ACATTGAGGC CTGGGAAGAT
    ACACATGCAA AGTGGGAACA GGCTACAAAA CTAATCCTTA ACTACCCAAA AGCCAAAATG
    GAAGATGGCC AGGCTGCAGA AAGTCTTCAT AAGACCATTG TTAAGAGACG GATGTCCCAT
    GTCAGTGGAG GAGGCTCCAT AGACTTGTCT GACACAGACT CCCTGCAGGA GTGGATCAAC
    ATGACTGGCT TCCTTTGTGC CCTTGGTGGG GTGTGCCTGC AACAGAGAAG CAGCTCTGGC
    CTAGCGACAT ACAGCCCACC TATGGGCGCT GTCAGTGAAC GCAAAGGGTC TATGATTTCT
    GTAATGTCTT CTGAAGGGAA TATTGATTCA CCTGTCAGCA GATTTATGGA CCGGCTTCTG
    TCCTTAATGG TGTGTAACCA CGAGAAAGTG GGGCTTCAGA TACGGACCAA TGTTAAGGAC
    CTGGTGGGTC TGGAGTTGAG TCCTGCTCTG TATCCAATGC TGTTTAACAA ACTGAAGAAT
    ACCATCAGCA AGTTTTTTGA CTCTCAAGGA CAGGTATTAC TAAGTGACAG CAATACTCAG
    TTTGTAGAGC AAACCATAGC CATAATGAAA AACCTACTGG ATAATCATAC TGAAGGCAGC
    TCTGAGCATC TGGGACAAGC GAGCATTGAG ACAATGATGC TAAATCTGGT CAGATACGTT
    CGTGTTCTTG GGAATATGGT CCATGCAATA CAAATAAAAA CAAAATTGTG TCAGTTAGTT
    GAAGTCATGA TGGCAAGAAG AGATGACCTC TCGTTTTGTC AAGAGATGAA ATTTAGGAAT
    AAGATGGTAG AATATCTAAC AGACTGGGTT ATGGGAACAT CAAACCAAGC AGCAGATGAT
    GACATAAAAT GTCTTACGAG AGATCTGGAC CAAGCAAGCA TGGAAGCAGT AGTTTCTCTC
    CTCGCTGGTC TTCCACTGCA GCCCGAAGAA GGAGATGGGG TGGAGCTGAT GGAGGCCAAA
    TCACAGTTAT TCCTTAAGTA CTTCACATTA TTTATGAACC TTTTGAATGA CTGTAGTGAA
    GTTGAAGATG AAAATGCACA AACGGGTGGC AGGAAACGTG GCATGTCTCG GAGGCTGGCA
    TCCCTGAGGC ACTGTACAGT GCTTGCAATG TCAAACTTAC TCAATGCTAA TGTGGACAGT
    GGACTCATGC ACTCCATAGG CTTAGGTTAT CACAAGGATC TTCAGACAAG AGCTACGTTT
    ATGGAAGTTC TGACAAAAAT CCTTCAACAA GGAACAGAAT TCGATACACT TGCGGAAACG
    GTGTTGGCAG ATCGATTTGA GAGACTTGTG GAACTGGTCA CAATGATGGG AGACCAGGGA
    GAACTCCCTA TAGCTATGGC TCTTGCCAAT GTGGTCCCTT GTTCTCAGTG GGATGAGCTG
    GCTCGGGTTC TGGTCACTCT GTTTGATTCC CGGCATTTAC TCTACCAGCT GCTCTGGAAC
    ATGTTTTCTA AGGAGGTAGA ATTGGCAGAC TCCATGCAGA CTCTCTTTCG AGGCAACAGC
    TTGGCCAGTA AGATAATGAC ATTCTGCTTC AAGGTGTATG GTGCTACTTA CCTACAAAAG
    CTCTTGGACC CTTTATTACG AGTCATCATC ACATCTTCGG ATTGGCAACA TGTTAGCTTT
    GAAGTGGATC CTACCAGGTT AGAACCCTCT GAGAGCCTTG AGGAGAACCA GAGGAACCTC
    CTTCAGATGA CAGAGAAGTT CTTCCATGCC ATCATCAGCT CTTCCTCAGA ATTCCCCTCA
    CAGCTTCGAA GTGTCTGCCA TTGTTTGTAC CAGGCAACTT GCCACTCCCT ACTGAATAAA
    GCTACAGTAA AAGAAAGAAA GGAAAACAAA AAATCAGTGG TTAGCCAGCG CTTCCCTCAG
    AACAGCATCG GTGCCGTAGG AAGTGCCATG TTTCTCAGAT TCATCAATCC TGCCATTGTC
    TCGCCGTATG AAGCAGGGAT TTTAGATAAA AAGCCACCAC CTAGAATTGA AAGGGGCTTG
    AAGTTAATGT CAAAGGTACT TCAGAGCATT GCCAATCATG TACTGTTCAC AAAAGAAGAG
    CATATGAGAC CTTTTAATGA TTTTGTGAAA AGCAACTTTG ACTTGGCACG AAGGTTTTTC
    CTAGATATAG CATCAGATTG TCCCACAAGT GATGCAGTAA ACCATAGTCT TTCCTTCATC
    AGCGATGGCA ATGTGCTTGC TTTACATCGG CTGCTTTGGA ACAATCAGGA GAAAATTGGC
    CAGTATCTTT CCAGTAACAG GGATCATAAA GCTGTTGGAA GACGACCTTT TGATAAGATG
    GCCACACTTC TCGCGTATCT GGGTCCTCCG GAGCACAAGC CTGTGGCAGA TACACACTGG
    TCCAGCCTTA ACCTTACCAG TTCAAAGTTT GAGGAATTTA TGACCAGGCA CCAGGTACAT
    GAGAAAGAAG AGTTCAAGGC CTTGAAAACG TTAAGCATCT TCTACCAAGC TGGCACCTCC
    AAAGCTGGGA ATCCTATTTT TTATTATGTT GCACGGAGGT TCAAAACTGG CCAGATCAAT
    GGTGATCTGC TGATATACCA TGTCTTGCTG ACTTTAAAGC CATATTATGC AAAGCCATAT
    GAAATTGTAG TGGACCTTAC CCATACTGGG CCTAGCAATC GCTTTAAAAC AGACTTCCTC
    TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT GCCTATGACA ACGTCTCTGC AGTCTATATC
    TATAACTGTA ACTCCTGGGT CAGGGAGTAT ACCAAATACC ATGAGCGGCT GCTGACTGGC
    CTCAAGGGCA GCAAAAGGCT CATTTTCATC GACTGTCCTG GGAAGCTGGC TGAACATATA
    GAGCATGAGC AACAGAAACT CCCTGCTGCT ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT
    TTCCACAATG CTCTCAAGTT AGCTCACAAG GACACCAAAG TTTCTATTAA GGTTGGTTCT
    ACTGCTGTTC AGGTAACTTC AGCAGAAAGA ACAAAAGTTT TGGGGCAATC CGTCTTTTTA
    AATGATATTT ACTATGCTTC TGAAATCGAA GAGATCTGCC TAGTTGATGA GAACCAGTTC
    ACTCTAACCA TTGCAAACCA AGGCACACCA CTGACCTTCA TGCACCAAGA ATGTGAAGCC
    ATTGTTCAGT CTATCATTCA TATCAGGACC CGCTGGGAGC TGTCACAACC TGACTCCATC
    CCTCAGCATA CCAAGATTCG ACCAAAGGAT GTCCCTGGGA CACTGCTTAA TATTGCGTTA
    CTGAATTTGG GCAGTTCAGA CCCTAGTTTA CGGTCTGCTG CCTATAATCT CCTGTGTGCC
    TTAACCTGCA CCTTTAATTT AAAGATTGAA GGTCAGTTGC TAGAGACATC GGGGTTATGC
    ATCCCTGCCA ACAACACACT CTTCATTGTC TCTATCAGTA AGACGCTCGC AGCCAATGAG
    CCACACCTCA CCTTAGAGTT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAATTGAAGC ACCTTTGTTT GGAATATATG ACCCCATGGC TGTCAAATCT AGTCCGTTTT
    TGTAAGCATA ATGATGATGC CAAACGACAA AGGGTTACTG CCATCCTTGA TAAGCTAATA
    ACAATGACTA TAAACGAGAA GCAGATGTAT CCTTCTATTC AAGCAAAAAT CTGGGGGAGC
    CTTGGGCAGA TCACAGACCT GCTGGATGTT GTGCTTGACA GTTTCATCAA GACCAGTGCG
    ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG GTGATGGCAG ACACAGCTGT GGCTTTAGCT
    TCTGGAAATG TGAAATTGGT GTCGAGTAAG GTTATTGGAA GGATGTGTAA AATAATTGAC
    AAGACTTGCT TATCCCCAAC TCCAACTTTA GAACAACATC TTATGTGGGA CGACATTGCC
    ATTTTAGCCC GCTACATGCT GATGCTGTCC TTCAACAACT CCCTCGATGT GGCGGCTCAT
    CTGCCCTATC TCTTCCATGT TGTCACTTTC TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA
    GCTTCCACAC ATGGGCTGCT CATCAATATC ATTCACTCTC TGTGTACTTG TTCTCAGCTT
    CACTTTAGTG AAGAGACCAA GCAAGTTTTG AGGCTCAGTC TAACAGAGTT CTCGTTACCC
    AAATTTTACT TACTGTTTGG CATTAGCAAA GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT
    TCCAGTTACC GGGACCGCTC CTTCTCCCCT GGCTCCTATG AGAGGGAGAC TTTTGCTTTG
    ACGTCCCTGG AAACAGTCAC AGAAGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT
    ATTCCAACAT GCAAGTGGCT GGATCAGTGG ACAGAACTAG CTCAAAGATT TGCGTTTCAG
    TATAACCCAT CGCTGCAGCC AAGAGCTCTT GTGGTGTTTG GCTGTATTAG CAAACGAGTG
    TCTCATGGGC AGATAAAGCA GATTATCCGA ATTCTTAGCA AGGCACTTGA AAGTTGTTTA
    AAAGGACCTG ACACTTACAA CAGTCAAGTT CTGATAGAAT CTACGGTGAT AGCACTAACA
    AAATTACAGC CGCTTCTTAA TAAGGACTCG CCCCTGCACA AAGCCCTCTT TTGGGTTGCT
    GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC TTGTATTCAG CCGGCACTGC ACTTCTGGAA
    CAAAACCTGC ACACCTTGGA CAGTCTCCGG ATATTCAATG ACAAGAGTCC GGAAGAAGTA
    TTTATGGCGA TCCGGAATCC TCTGGAATGG CACTGCAAGC AGATGGATCA CTTTGTCGGA
    CTCAACTTCA ACTCTAACTT TAACTTTGCA CTAGTTGGAC ACCTCTTAAA AGGGTACAGG
    CATCCTTCAC CTGCCATTGT TGCAAGAACA GTCAGAATTT TGCATACACT ACTAACTCTC
    GTTAACAAAC ATAGAAATTG TGACAAATTT GAGGTGAATA CACAGAGTGT GGCTTACTTA
    GCAGCTCTAC TCACAGTGTC TGAAGAGGTT CGAAGTCGCT GTAGCCTCAA GCATAGGAAG
    TCCCTTCTTC TTACTGATAT TTCAATGGAA AATGTTCCTA TGGATACATA TCCCATTCAT
    CACGGTGACC CCAGCTATAG GACACTAAAG GAGACCCAGC CATGGTCCTC TCCCAAAGGT
    TCAGAAGGAT ACCTTGCAGC CACCTACCCA GCTGTAGGCC AAACCAGTCC CCGAGCCAGG
    AAATCCATGA GCTTGGATAT GGGACAGCCT TCTCAGGCCA ACACAAAAAA ATTGCTTGGA
    ACAAGAAAAA GTTTTGATCA CTTGATATCT GACACAAAGG CTCCTAAAAG ACAAGAAATG
    GAATCAGGGA TTACAACACC CCCGAAAATG AGAAGAGTGG CAGAGACTGA CTATGAAATG
    GCAGAAACCC AAAGGATTCC CTCCTCGCAG CAGCACCCAC ATTTACGGAA AGTCTCAGTC
    TCTGAATCAA ATGTTCTTTT GGATGAAGAA GTTCTTACCG ACCCAAAGAT CCAAGCGCTG
    CTTCTTACTG TTCTGGCTAC ACTGGTAAAA TATACTACAG ATGAATTTGA TCAACGGATT
    CTTTATGAAT ATCTAGCAGA GGCCAGCGTT GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC
    AATTTGTTGG ACTCTAAGAT CAATACCCTC TTGTCTTTAT GCCAAGATCC AAATTTGTTA
    AATCCAATCC ATGGAATTGT ACAGAGTGTG GTGTACCATG AGGAGTCCCC GCCACAGTAC
    CAGACATCTT ACCTGCAAAG TTTTGGTTTT AATGGCTTAT GGCGGTTTGC AGGACCCTTT
    TCAAAGCAAA CACAAATCCC TGACTATGCT GAGCTCATTG TTAAATTTCT TGATGCCTTG
    ATTGACACGT ACTTGCCTGG AATTGATGAA GAAACCAGTG AGGAGTCCCT CCTGACACCC
    ACATCTCCTT ACCCTCCTGC ACTGCAGAGC CAGCTTAGTA TCACTGCCAA CCTTAACCTC
    TCTAATTCCA TGACCTCACT TGCAACTTCC CAGCATTCCC CAGGACTCGA CAAGGAGAAC
    GTTGAACTCT CCCCCACCGC TGGCCACTGT AACAGTGGAC GGACTCGCCA TGGATCCGCA
    AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC AGTTTCAAAC GTAATAGCAT TAAGAAGATC
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101504.1
    CDS407..8932
    Translation

    Target ORF information:

    RefSeq Version XM_030245644.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus neurofibromin 1 (Nf1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCAC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AGACAGGACA GCAGAACACA CATACCAAAG TCAGCACCGA GCACAACAAG
    GAGTGTCTGA TCAACATTTC CAAATACAAG TTTTCTCTGG TCATCAGTGG GCTCACCACC
    ATCCTAAAGA ATGTTAACAA TATGAGGATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATCATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAGAC AATGCTGGTC AAACAATTAC TACCAGAAAT CTGCCATTTT
    CTTCACACCT GCCGTGAAGG AAACCAACAT GCAGCCGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACAAGG
    TTACAGGAGT TGACTGTCTG TTCAGAAGAT AATGTTGATG TTCACGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTTC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TGAAGAAGGT TGCACAGTTG GCAGTTATAA ATAGCCTAGA AAAGGCTTTT
    TGGAACTGGG TAGAAAATTA TCCGGATGAA TTTACAAAGC TATACCAAAT TCCACAGACA
    GATATGGCTG AATGTGCAGA GAAGCTGTTT GACTTGGTGG ATGGTTTTGC AGAAAGCACC
    AAACGTAAAG CAGCAGTGTG GCCCCTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGACA TCTCCAAGGA TGTGGTGGAC GAGAGCAACA TAAACAAGAA GCTGTTTCTG
    GACAGTTTAC GGAAAGCACT CGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAGAGTGCT
    GCCATTGCTT GTGTCAAGTT GTGTAAAGCA AGTACTTACA TCAACTGGGA AGATAATTCT
    GTCATTTTCC TACTGGTTCA GTCCATGGTA GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCTAGAGG CAGTCAGCCT GCAGATGTGG ACCTCATGAT TGACTGTCTT
    GTCTCTTGCT TTCGGATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGTTG GTAAACTCAC TGCACCGCAT CATCACCAAT
    TCTGCGTTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GTCACTCAGT TGAACTTCGC
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGCT GTGGAGCGCA CCCAGCAATA
    CGAATGGCAC CAAGTCTCAC CTTTAAAGAA AAGGTAACAA GCCTTAAATT TAAAGAAAAG
    CCTACAGACC TAGAGACAAG GAGCTACAAA TGTCTTCTTT TGTCCATGGT GAAACTGATT
    CATGCAGACC CAAAGCTTTT GCTTTGTAAT CCAAGAAAAC AGGGCCCTGA AACTCAGAGC
    AGTACAGCAG AACTAATTAC AGGGCTCGTA CAACTGGTTC CTCAGTCACA CATGCCAGAG
    GTCGCTCAGG AGGCTATGGA GGCTTTGCTG GTCCTTCATC AGTTAGATAG CATTGATCTC
    TGGAATCCTG ACGCTCCTGT AGAGACATTC TGGGAAATCA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG CCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTA
    CGGGAAATTC TGATCTGCAG AAATAAATTT CTTCTTAAAA ATAAGGCAGA TAGAAGCTCC
    TGTCATTCTC TCTACCTTTA TGGAGTAGGA TGTGAAATGT CTGCTACTGG AAATACCACT
    CAGATGTCAG TTGATCATGA TGAGTTCCTG CGTGCCTGTA CTCCTGGAGC ATCTCTGCGG
    AAAGGAAGAG GGAATTCCTC CATGGATAGC ACAGCAGGGT GCAGTGGAAC CCCACCTATA
    TGCCGACAAG CCCAGACCAA GCTAGAAGTG GCCTTATACA TGTTTCTGTG GAATCCTGAC
    ACTGAAGCTG TTCTGGTTGC TATGTCCTGT TTCCGCCACC TCTGTGAAGA AGCAGATATT
    CGATGTGGGG TAGATGAAGT GTCAGTACAC AACTTCTTGC CCAACTATAA CACATTCATG
    GAATTTGCCT CAGTCAGCAA TATGATGTCA ACAGGAAGAG CAGCGCTTCA GAAAAGAGTG
    ATGGCCCTGC TAAGGCGCAT TGAGCACCCT ACTGCAGGAA ACATTGAGGC CTGGGAAGAT
    ACACATGCAA AGTGGGAACA GGCTACAAAA CTAATCCTTA ACTACCCAAA AGCCAAAATG
    GAAGATGGCC AGGCTGCAGA AAGTCTTCAT AAGACCATTG TTAAGAGACG GATGTCCCAT
    GTCAGTGGAG GAGGCTCCAT AGACTTGTCT GACACAGACT CCCTGCAGGA GTGGATCAAC
    ATGACTGGCT TCCTTTGTGC CCTTGGTGGG GTGTGCCTGC AACAGAGAAG CAGCTCTGGC
    CTAGCGACAT ACAGCCCACC TATGGGCGCT GTCAGTGAAC GCAAAGGGTC TATGATTTCT
    GTAATGTCTT CTGAAGGGAA TATTGATTCA CCTGTCAGCA GATTTATGGA CCGGCTTCTG
    TCCTTAATGG TGTGTAACCA CGAGAAAGTG GGGCTTCAGA TACGGACCAA TGTTAAGGAC
    CTGGTGGGTC TGGAGTTGAG TCCTGCTCTG TATCCAATGC TGTTTAACAA ACTGAAGAAT
    ACCATCAGCA AGTTTTTTGA CTCTCAAGGA CAGGTATTAC TAAGTGACAG CAATACTCAG
    TTTGTAGAGC AAACCATAGC CATAATGAAA AACCTACTGG ATAATCATAC TGAAGGCAGC
    TCTGAGCATC TGGGACAAGC GAGCATTGAG ACAATGATGC TAAATCTGGT CAGATACGTT
    CGTGTTCTTG GGAATATGGT CCATGCAATA CAAATAAAAA CAAAATTGTG TCAGTTAGTT
    GAAGTCATGA TGGCAAGAAG AGATGACCTC TCGTTTTGTC AAGAGATGAA ATTTAGGAAT
    AAGATGGTAG AATATCTAAC AGACTGGGTT ATGGGAACAT CAAACCAAGC AGCAGATGAT
    GACATAAAAT GTCTTACGAG AGATCTGGAC CAAGCAAGCA TGGAAGCAGT AGTTTCTCTC
    CTCGCTGGTC TTCCACTGCA GCCCGAAGAA GGAGATGGGG TGGAGCTGAT GGAGGCCAAA
    TCACAGTTAT TCCTTAAGTA CTTCACATTA TTTATGAACC TTTTGAATGA CTGTAGTGAA
    GTTGAAGATG AAAATGCACA AACGGGTGGC AGGAAACGTG GCATGTCTCG GAGGCTGGCA
    TCCCTGAGGC ACTGTACAGT GCTTGCAATG TCAAACTTAC TCAATGCTAA TGTGGACAGT
    GGACTCATGC ACTCCATAGG CTTAGGTTAT CACAAGGATC TTCAGACAAG AGCTACGTTT
    ATGGAAGTTC TGACAAAAAT CCTTCAACAA GGAACAGAAT TCGATACACT TGCGGAAACG
    GTGTTGGCAG ATCGATTTGA GAGACTTGTG GAACTGGTCA CAATGATGGG AGACCAGGGA
    GAACTCCCTA TAGCTATGGC TCTTGCCAAT GTGGTCCCTT GTTCTCAGTG GGATGAGCTG
    GCTCGGGTTC TGGTCACTCT GTTTGATTCC CGGCATTTAC TCTACCAGCT GCTCTGGAAC
    ATGTTTTCTA AGGAGGTAGA ATTGGCAGAC TCCATGCAGA CTCTCTTTCG AGGCAACAGC
    TTGGCCAGTA AGATAATGAC ATTCTGCTTC AAGGTGTATG GTGCTACTTA CCTACAAAAG
    CTCTTGGACC CTTTATTACG AGTCATCATC ACATCTTCGG ATTGGCAACA TGTTAGCTTT
    GAAGTGGATC CTACCAGGTT AGAACCCTCT GAGAGCCTTG AGGAGAACCA GAGGAACCTC
    CTTCAGATGA CAGAGAAGTT CTTCCATGCC ATCATCAGCT CTTCCTCAGA ATTCCCCTCA
    CAGCTTCGAA GTGTCTGCCA TTGTTTGTAC CAGGCAACTT GCCACTCCCT ACTGAATAAA
    GCTACAGTAA AAGAAAGAAA GGAAAACAAA AAATCAGTGG TTAGCCAGCG CTTCCCTCAG
    AACAGCATCG GTGCCGTAGG AAGTGCCATG TTTCTCAGAT TCATCAATCC TGCCATTGTC
    TCGCCGTATG AAGCAGGGAT TTTAGATAAA AAGCCACCAC CTAGAATTGA AAGGGGCTTG
    AAGTTAATGT CAAAGGTACT TCAGAGCATT GCCAATCATG TACTGTTCAC AAAAGAAGAG
    CATATGAGAC CTTTTAATGA TTTTGTGAAA AGCAACTTTG ACTTGGCACG AAGGTTTTTC
    CTAGATATAG CATCAGATTG TCCCACAAGT GATGCAGTAA ACCATAGTCT TTCCTTCATC
    AGCGATGGCA ATGTGCTTGC TTTACATCGG CTGCTTTGGA ACAATCAGGA GAAAATTGGC
    CAGTATCTTT CCAGTAACAG GGATCATAAA GCTGTTGGAA GACGACCTTT TGATAAGATG
    GCCACACTTC TCGCGTATCT GGGTCCTCCG GAGCACAAGC CTGTGGCAGA TACACACTGG
    TCCAGCCTTA ACCTTACCAG TTCAAAGTTT GAGGAATTTA TGACCAGGCA CCAGGTACAT
    GAGAAAGAAG AGTTCAAGGC CTTGAAAACG TTAAGCATCT TCTACCAAGC TGGCACCTCC
    AAAGCTGGGA ATCCTATTTT TTATTATGTT GCACGGAGGT TCAAAACTGG CCAGATCAAT
    GGTGATCTGC TGATATACCA TGTCTTGCTG ACTTTAAAGC CATATTATGC AAAGCCATAT
    GAAATTGTAG TGGACCTTAC CCATACTGGG CCTAGCAATC GCTTTAAAAC AGACTTCCTC
    TCTAAGTGGT TTGTTGTCTT TCCTGGCTTT GCCTATGACA ACGTCTCTGC AGTCTATATC
    TATAACTGTA ACTCCTGGGT CAGGGAGTAT ACCAAATACC ATGAGCGGCT GCTGACTGGC
    CTCAAGGGCA GCAAAAGGCT CATTTTCATC GACTGTCCTG GGAAGCTGGC TGAACATATA
    GAGCATGAGC AACAGAAACT CCCTGCTGCT ACCTTGGCTT TGGAAGAAGA TCTAAAGGTT
    TTCCACAATG CTCTCAAGTT AGCTCACAAG GACACCAAAG TTTCTATTAA GGTTGGTTCT
    ACTGCTGTTC AGGTAACTTC AGCAGAAAGA ACAAAAGTTT TGGGGCAATC CGTCTTTTTA
    AATGATATTT ACTATGCTTC TGAAATCGAA GAGATCTGCC TAGTTGATGA GAACCAGTTC
    ACTCTAACCA TTGCAAACCA AGGCACACCA CTGACCTTCA TGCACCAAGA ATGTGAAGCC
    ATTGTTCAGT CTATCATTCA TATCAGGACC CGCTGGGAGC TGTCACAACC TGACTCCATC
    CCTCAGCATA CCAAGATTCG ACCAAAGGAT GTCCCTGGGA CACTGCTTAA TATTGCGTTA
    CTGAATTTGG GCAGTTCAGA CCCTAGTTTA CGGTCTGCTG CCTATAATCT CCTGTGTGCC
    TTAACCTGCA CCTTTAATTT AAAGATTGAA GGTCAGTTGC TAGAGACATC GGGGTTATGC
    ATCCCTGCCA ACAACACACT CTTCATTGTC TCTATCAGTA AGACGCTCGC AGCCAATGAG
    CCACACCTCA CCTTAGAGTT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAATTGAAGC ACCTTTGTTT GGAATATATG ACCCCATGGC TGTCAAATCT AGTCCGTTTT
    TGTAAGCATA ATGATGATGC CAAACGACAA AGGGTTACTG CCATCCTTGA TAAGCTAATA
    ACAATGACTA TAAACGAGAA GCAGATGTAT CCTTCTATTC AAGCAAAAAT CTGGGGGAGC
    CTTGGGCAGA TCACAGACCT GCTGGATGTT GTGCTTGACA GTTTCATCAA GACCAGTGCG
    ACAGGAGGCT TAGGGTCTAT CAAAGCTGAG GTGATGGCAG ACACAGCTGT GGCTTTAGCT
    TCTGGAAATG TGAAATTGGT GTCGAGTAAG GTTATTGGAA GGATGTGTAA AATAATTGAC
    AAGACTTGCT TATCCCCAAC TCCAACTTTA GAACAACATC TTATGTGGGA CGACATTGCC
    ATTTTAGCCC GCTACATGCT GATGCTGTCC TTCAACAACT CCCTCGATGT GGCGGCTCAT
    CTGCCCTATC TCTTCCATGT TGTCACTTTC TTAGTAGCCA CAGGTCCCTT GTCCCTCCGA
    GCTTCCACAC ATGGGCTGCT CATCAATATC ATTCACTCTC TGTGTACTTG TTCTCAGCTT
    CACTTTAGTG AAGAGACCAA GCAAGTTTTG AGGCTCAGTC TAACAGAGTT CTCGTTACCC
    AAATTTTACT TACTGTTTGG CATTAGCAAA GTCAAGTCGG CTGCTGTCAT TGCCTTCCGT
    TCCAGTTACC GGGACCGCTC CTTCTCCCCT GGCTCCTATG AGAGGGAGAC TTTTGCTTTG
    ACGTCCCTGG AAACAGTCAC AGAAGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT
    ATTCCAACAT GCAAGTGGCT GGATCAGTGG ACAGAACTAG CTCAAAGATT TGCGTTTCAG
    TATAACCCAT CGCTGCAGCC AAGAGCTCTT GTGGTGTTTG GCTGTATTAG CAAACGAGTG
    TCTCATGGGC AGATAAAGCA GATTATCCGA ATTCTTAGCA AGGCACTTGA AAGTTGTTTA
    AAAGGACCTG ACACTTACAA CAGTCAAGTT CTGATAGAAT CTACGGTGAT AGCACTAACA
    AAATTACAGC CGCTTCTTAA TAAGGACTCG CCCCTGCACA AAGCCCTCTT TTGGGTTGCT
    GTGGCTGTGC TGCAGCTGGA CGAAGTCAAC TTGTATTCAG CCGGCACTGC ACTTCTGGAA
    CAAAACCTGC ACACCTTGGA CAGTCTCCGG ATATTCAATG ACAAGAGTCC GGAAGAAGTA
    TTTATGGCGA TCCGGAATCC TCTGGAATGG CACTGCAAGC AGATGGATCA CTTTGTCGGA
    CTCAACTTCA ACTCTAACTT TAACTTTGCA CTAGTTGGAC ACCTCTTAAA AGGGTACAGG
    CATCCTTCAC CTGCCATTGT TGCAAGAACA GTCAGAATTT TGCATACACT ACTAACTCTC
    GTTAACAAAC ATAGAAATTG TGACAAATTT GAGGTGAATA CACAGAGTGT GGCTTACTTA
    GCAGCTCTAC TCACAGTGTC TGAAGAGGTT CGAAGTCGCT GTAGCCTCAA GCATAGGAAG
    TCCCTTCTTC TTACTGATAT TTCAATGGAA AATGTTCCTA TGGATACATA TCCCATTCAT
    CACGGTGACC CCAGCTATAG GACACTAAAG GAGACCCAGC CATGGTCCTC TCCCAAAGGT
    TCAGAAGGAT ACCTTGCAGC CACCTACCCA GCTGTAGGCC AAACCAGTCC CCGAGCCAGG
    AAATCCATGA GCTTGGATAT GGGACAGCCT TCTCAGGCCA ACACAAAAAA ATTGCTTGGA
    ACAAGAAAAA GTTTTGATCA CTTGATATCT GACACAAAGG CTCCTAAAAG ACAAGAAATG
    GAATCAGGGA TTACAACACC CCCGAAAATG AGAAGAGTGG CAGAGACTGA CTATGAAATG
    GCAGAAACCC AAAGGATTCC CTCCTCGCAG CAGCACCCAC ATTTACGGAA AGTCTCAGTC
    TCTGAATCAA ATGTTCTTTT GGATGAAGAA GTTCTTACCG ACCCAAAGAT CCAAGCGCTG
    CTTCTTACTG TTCTGGCTAC ACTGGTAAAA TATACTACAG ATGAATTTGA TCAACGGATT
    CTTTATGAAT ATCTAGCAGA GGCCAGCGTT GTGTTTCCCA AAGTGTTTCC TGTTGTGCAC
    AATTTGTTGG ACTCTAAGAT CAATACCCTC TTGTCTTTAT GCCAAGATCC AAATTTGTTA
    AATCCAATCC ATGGAATTGT ACAGAGTGTG GTGTACCATG AGGAGTCCCC GCCACAGTAC
    CAGACATCTT ACCTGCAAAG TTTTGGTTTT AATGGCTTAT GGCGGTTTGC AGGACCCTTT
    TCAAAGCAAA CACAAATCCC TGACTATGCT GAGCTCATTG TTAAATTTCT TGATGCCTTG
    ATTGACACGT ACTTGCCTGG AATTGATGAA GAAACCAGTG AGGAGTCCCT CCTGACACCC
    ACATCTCCTT ACCCTCCTGC ACTGCAGAGC CAGCTTAGTA TCACTGCCAA CCTTAACCTC
    TCTAATTCCA TGACCTCACT TGCAACTTCC CAGCATTCCC CAGGACTCGA CAAGGAGAAC
    GTTGAACTCT CCCCCACCGC TGGCCACTGT AACAGTGGAC GGACTCGCCA TGGATCCGCA
    AGCCAAGTGC AGAAGCAAAG AAGTGCTGGC AGTTTCAAAC GTAATAGCAT TAAGAAGATC
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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