Nlk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nlk
Entrez Gene ID	18099
Full Name	nemo like kinase
Synonyms	AI194375
General protein information	Preferred Names nemo like kinase Names serine/threonine-protein kinase NLK nlk
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_008702.3	NP_032728.3	serine/threonine-protein kinase NLK

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nlk	NP_032728.3
Homo sapiens (human)	NLK	NP_057315.3
Gallus gallus (chicken)	NLK	XP_415915.3
Drosophila melanogaster (fruit fly)	nmo	NP_729318.1
Caenorhabditis elegans (roundworm)	lit-1	NP_001022806.1
Rattus norvegicus (Norway rat)	Nlk	NP_001178853.1
Danio rerio (zebrafish)	nlk2	NP_001106957.1
Pan troglodytes (chimpanzee)	NLK	XP_001147662.2
Canis lupus familiaris (dog)	NLK	XP_868108.2
Bos taurus (cattle)	NLK	NP_001180182.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005898	XP_315928.3
Xenopus tropicalis (tropical clawed frog)	nlk	NP_001116885.1

Gene Ontology
Bibliography

Related articles in PubMed

Wip1 directly dephosphorylates NLK and increases Wnt activity during germ cell development.
Cho SJ, Cha BS, Kwon OS, Lim J, Shin DM, Han DW, Ishitani T, Jho EH, Fornace AJ, Cha HJ
Biochimica et biophysica acta1863(4)1013-1022(2017 Apr)

Correlated evolution between CK1? Protein and the Serine-rich Motif Contributes to Regulating the Mammalian Circadian Clock.
Xing L, An Y, Shi G, Yan J, Xie P, Qu Z, Zhang Z, Liu Z, Pan D, Xu Y
The Journal of biological chemistry292(1)161-171(2017 Jan)

Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization.
Moon S, Kim W, Kim S, Kim Y, Song Y, Bilousov O, Kim J, Lee T, Cha B, Kim M, Kim H, Katanaev VL, Jho EH
EMBO reports18(1)61-71(2017 01)

Nemo-Like Kinase (NLK) Is a Pathological Signaling Effector in the Mouse Heart.
Liu R, Khalil H, Lin SJ, Sargent MA, York AJ, Molkentin JD
PloS one11(10)e0164897(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our data suggest that NLK/Nemo acts as an endogenous regulator of Hippo signaling by controlling nuclear localization and activity of YAP/Yorkie.
Title: Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization.

NLK is a novel signaling molecule for proper lung development through the interconnection between epithelial and endothelial cells during lung morphogenesis
Title: Nemo-like kinase regulates the expression of vascular endothelial growth factor (VEGF) in alveolar epithelial cells.

NLK can phosphorylate the mutant polyglutamine-expanded androgen receptor protein, enhance its aggregation, and promote androgen receptor-dependent gene transcription by regulating androgen receptor-cofactor interactions.
Title: Nemo-like kinase is a novel regulator of spinal and bulbar muscular atrophy.

Nlk is a negative regulator of skeletal homeostasis.
Title: Nemo-like kinase regulates postnatal skeletal homeostasis.

either reducing NLK enzymatic activity or decreasing NLK expression levels can have beneficial effects against the toxicity induced by polyglutamine-expanded ATXN1
Title: Polyglutamine disease toxicity is regulated by Nemo-like kinase in spinocerebellar ataxia type 1.

The following Nlk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02584	NM_008702.3 Latest version!	Mus musculus nemo like kinase (Nlk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nlk ( NM_008702.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032728.3 Mus musculus nemo like kinase (Nlk), mRNA.

Price & Availability↑

CloneID	OMu02584
Clone ID Related Accession (Same CDS sequence)	NM_008702.3
Accession Version	NM_008702.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase NLK
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY137033.1, BY237029.1, BU923375.1, AK078118.1, CB234984.1, AK160340.1 and CO042120.1. On Jun 22, 2007 this sequence version replaced NM_008702.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058652.1, SRR1660819.30727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTTT GTGGCACAAG AGCCAACGCA AAAATGATGG CGGCTTACAA TGGCGGTACA 
TCTGCAGCTG CAGCAGGTCA CCACCACCAC CATCATCACC ACCTTCCACA CCTTCCTCCT 
CCTCACCTTC ACCACCACCA CCACCCTCAA CACCACCTTC ATCCGGGGTC GGCTGCTGCC 
GTACACCCAG TCCAGCAGCA TACGTCTTCG GCAGCTGCGG CAGCCGCCGC AGCAGCTGCA 
GCCGCAGCCA TGTTAAACCC TGGGCAACAA CAGCCATATT TCCCATCCCC GGCACCGGGT 
CAGGCTCCTG GGCCAGCCGC AGCAGCCCCA GCTCAGGTAC AGGCTGCTGC AGCTGCTACA 
GTTAAGGCGC ACCATCATCA GCACTCGCAT CATCCGCAAC AGCAGCTGGA CATTGAGCCG 
GATAGACCAA TTGGATATGG AGCCTTTGGC GTTGTCTGGT CAGTAACAGA TCCAAGAGAT 
GGAAAGAGAG TAGCACTCAA AAAGATGCCC AACGTCTTCC AGAATCTGGT CTCTTGCAAA 
AGAGTCTTCC GGGAATTGAA GATGTTGTGT TTTTTTAAAC ATGATAATGT ACTCTCTGCC 
CTTGACATAC TCCAACCTCC ACACATTGAC TATTTTGAAG AAATATATGT TGTCACAGAA 
TTGATGCAGA GTGATCTACA TAAAATTATC GTCTCTCCTC AGCCACTCAG CTCGGATCAT 
GTCAAAGTTT TTCTTTATCA GATTTTGCGA GGTTTGAAAT ATCTCCATTC AGCTGGCATT 
TTGCATCGAG ACATTAAGCC AGGGAACCTC CTTGTGAACA GCAATTGTGT TCTAAAGATT 
TGTGATTTTG GATTGGCCAG AGTGGAGGAA TTGGATGAAT CCCGTCATAT GACTCAGGAA 
GTAGTTACTC AGTATTACCG GGCTCCGGAG ATCCTGATGG GCAGCCGTCA TTACAGCAAT 
GCTATCGACA TCTGGTCTGT CGGGTGCATC TTTGCAGAGC TACTGGGCCG AAGAATATTG 
TTTCAGGCGC AGAGTCCCAT TCAGCAGTTG GATTTGATCA CAGATCTGTT GGGCACACCA 
TCACTGGAAG CAATGAGGAC AGCTTGTGAA GGTGCTAAGG CACACATACT CAGGGGTCCT 
CATAAACAGC CATCTCTTCC TGTGCTCTAC ACCCTCTCCA GCCAGGCCAC ACATGAAGCT 
GTTCACCTGC TTTGCAGGAT GTTGGTCTTT GATCCATCAA AAAGAATATC CGCTAAGGAT 
GCCTTAGCCC ACCCCTACCT AGATGAAGGG CGGCTGAGAT ACCATACGTG TATGTGCAAG 
TGCTGCTTTT CCACCTCAAC GGGGAGAGTC TACACCAGTG ACTTTGAGCC TGTCACCAAC 
CCCAAATTTG ATGACACCTT TGAGAAGAAC CTCAGCTCTG TCCGACAGGT TAAAGAAATT 
ATCCACCAGT TCATTCTGGA ACAGCAGAAA GGAAACAGAG TGCCTCTCTG CATCAACCCG 
CAGTCCGCTG CCTTTAAGAG CTTTATTAGT TCCACCGTCG CTCAGCCGTC TGAGATGCCG 
CCGTCTCCTC TGGTGTGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032728.3
CDS	1160..2743
Translation	MSLCGTRANAKMMAAYNGGTSAAAAGHHHHHHHHLPHLPPPHLHHHHHPQHHLHPGSAAAVHPVQQHTSSAAAAAAAAAAAAAMLNPGQQQPYFPSPAPGQAPGPAAAAPAQVQAAAAATVKAHHHQHSHHPQQQLDIEPDRPIGYGAFGVVWSVTDPRDGKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSDLHKIIVSPQPLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCVLKICDFGLARVEELDESRHMTQEVVTQYYRAPEILMGSRHYSNAIDIWSVGCIFAELLGRRILFQAQSPIQQLDLITDLLGTPSLEAMRTACEGAKAHILRGPHKQPSLPVLYTLSSQATHEAVHLLCRMLVFDPSKRISAKDALAHPYLDEGRLRYHTCMCKCCFSTSTGRVYTSDFEPVTNPKFDDTFEKNLSSVRQVKEIIHQFILEQQKGNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLVWE

Target ORF information:

RefSeq Version	NM_008702.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nemo like kinase (Nlk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008702.3

ORF Insert Sequence:

ATGTCTCTTT GTGGCACAAG AGCCAACGCA AAAATGATGG CGGCTTACAA TGGCGGTACA 
TCTGCAGCTG CAGCAGGTCA CCACCACCAC CATCATCACC ACCTTCCACA CCTTCCTCCT 
CCTCACCTTC ACCACCACCA CCACCCTCAA CACCACCTTC ATCCGGGGTC GGCTGCTGCC 
GTACACCCAG TCCAGCAGCA TACGTCTTCG GCAGCTGCGG CAGCCGCCGC AGCAGCTGCA 
GCCGCAGCCA TGTTAAACCC TGGGCAACAA CAGCCATATT TCCCATCCCC GGCACCGGGT 
CAGGCTCCTG GGCCAGCCGC AGCAGCCCCA GCTCAGGTAC AGGCTGCTGC AGCTGCTACA 
GTTAAGGCGC ACCATCATCA GCACTCGCAT CATCCGCAAC AGCAGCTGGA CATTGAGCCG 
GATAGACCAA TTGGATATGG AGCCTTTGGC GTTGTCTGGT CAGTAACAGA TCCAAGAGAT 
GGAAAGAGAG TAGCACTCAA AAAGATGCCC AACGTCTTCC AGAATCTGGT CTCTTGCAAA 
AGAGTCTTCC GGGAATTGAA GATGTTGTGT TTTTTTAAAC ATGATAATGT ACTCTCTGCC 
CTTGACATAC TCCAACCTCC ACACATTGAC TATTTTGAAG AAATATATGT TGTCACAGAA 
TTGATGCAGA GTGATCTACA TAAAATTATC GTCTCTCCTC AGCCACTCAG CTCGGATCAT 
GTCAAAGTTT TTCTTTATCA GATTTTGCGA GGTTTGAAAT ATCTCCATTC AGCTGGCATT 
TTGCATCGAG ACATTAAGCC AGGGAACCTC CTTGTGAACA GCAATTGTGT TCTAAAGATT 
TGTGATTTTG GATTGGCCAG AGTGGAGGAA TTGGATGAAT CCCGTCATAT GACTCAGGAA 
GTAGTTACTC AGTATTACCG GGCTCCGGAG ATCCTGATGG GCAGCCGTCA TTACAGCAAT 
GCTATCGACA TCTGGTCTGT CGGGTGCATC TTTGCAGAGC TACTGGGCCG AAGAATATTG 
TTTCAGGCGC AGAGTCCCAT TCAGCAGTTG GATTTGATCA CAGATCTGTT GGGCACACCA 
TCACTGGAAG CAATGAGGAC AGCTTGTGAA GGTGCTAAGG CACACATACT CAGGGGTCCT 
CATAAACAGC CATCTCTTCC TGTGCTCTAC ACCCTCTCCA GCCAGGCCAC ACATGAAGCT 
GTTCACCTGC TTTGCAGGAT GTTGGTCTTT GATCCATCAA AAAGAATATC CGCTAAGGAT 
GCCTTAGCCC ACCCCTACCT AGATGAAGGG CGGCTGAGAT ACCATACGTG TATGTGCAAG 
TGCTGCTTTT CCACCTCAAC GGGGAGAGTC TACACCAGTG ACTTTGAGCC TGTCACCAAC 
CCCAAATTTG ATGACACCTT TGAGAAGAAC CTCAGCTCTG TCCGACAGGT TAAAGAAATT 
ATCCACCAGT TCATTCTGGA ACAGCAGAAA GGAAACAGAG TGCCTCTCTG CATCAACCCG 
CAGTCCGCTG CCTTTAAGAG CTTTATTAGT TCCACCGTCG CTCAGCCGTC TGAGATGCCG 
CCGTCTCCTC TGGTGTGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Wip1 directly dephosphorylates NLK and increases Wnt activity during germ cell development.
Biochimica et biophysica acta1863(4)1013-1022(2017 Apr)
Cho SJ,Cha BS,Kwon OS,Lim J,Shin DM,Han DW,Ishitani T,Jho EH,Fornace AJ,Cha HJ

Correlated evolution between CK1? Protein and the Serine-rich Motif Contributes to Regulating the Mammalian Circadian Clock.
The Journal of biological chemistry292(1)161-171(2017 Jan)
Xing L,An Y,Shi G,Yan J,Xie P,Qu Z,Zhang Z,Liu Z,Pan D,Xu Y

Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization.
EMBO reports18(1)61-71(2017 01)
Moon S,Kim W,Kim S,Kim Y,Song Y,Bilousov O,Kim J,Lee T,Cha B,Kim M,Kim H,Katanaev VL,Jho EH

Nemo-Like Kinase (NLK) Is a Pathological Signaling Effector in the Mouse Heart.
PloS one11(10)e0164897(2016)
Liu R,Khalil H,Lin SJ,Sargent MA,York AJ,Molkentin JD

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

Nlk cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM

SUPPORT

COMPANY

SUPPORT

COMPANY