Nsg1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Nsg1
Entrez Gene ID	18196
Full Name	neuron specific gene family member 1
Synonyms	Neep21,m234,p21
General protein information	Preferred Names neuron specific gene family member 1 Names neuronal vesicle trafficking-associated protein 1 neuron-specific protein family member 1 brain neuron cytoplasmic protein 1 neuron-enriched endosomal protein of 21 kDa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 B3\|5 20.21 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010942.3	NP_035072.2	neuron-specific protein family member 1
NM_001359090.1	NP_001346019.1	neuronal vesicle trafficking-associated protein 1
NM_010942.4	NP_035072.2	neuronal vesicle trafficking-associated protein 1
NM_001359091.1	NP_001346020.1	neuronal vesicle trafficking-associated protein 1
XM_006503773.1	XP_006503836.1	neuron-specific protein family member 1 isoform X1
NM_001359092.1	NP_001346021.1	neuronal vesicle trafficking-associated protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NSG1	XP_536243.4
Mus musculus (house mouse)	Nsg1	NP_035072.2
Homo sapiens (human)	NSG1	NP_055207.1
Pan troglodytes (chimpanzee)	LOC461091	XP_001154737.2
Gallus gallus (chicken)	NSG1	NP_989896.1
	NSG1	XP_001095468.2
Rattus norvegicus (Norway rat)	Nsg1	NP_077042.2
Bos taurus (cattle)	NSG1	NP_001071425.1
Xenopus tropicalis (tropical clawed frog)	nsg1	NP_001072849.1

Gene Ontology
Bibliography

Related articles in PubMed

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Rutledge EA, Benazet JD, McMahon AP
Development (Cambridge, England)144(17)3177-3188(2017 09)

The endosomal neuronal proteins Nsg1/NEEP21 and Nsg2/P19 are itinerant, not resident proteins of dendritic endosomes.
Yap CC, Digilio L, McMahon L, Winckler B
Scientific reports7(1)10481(2017 Sep)

The related neuronal endosomal proteins NEEP21 (Nsg1) and P19 (Nsg2) have divergent expression profiles in vivo.
Barford K, Yap CC, Dwyer ND, Winckler B
The Journal of comparative neurology525(8)1861-1878(2017 Jun)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we show that NEEP21/Nsg1 and P19/Nsg2 are present in both overlapping and distinct cell populations in the hippocampus, neocortex, and cerebellum during development.
Title: The related neuronal endosomal proteins NEEP21 (Nsg1) and P19 (Nsg2) have divergent expression profiles in vivo.

Expression of Nsg1 in the mouse nucleus accumbens is modulated by a sequence variant (B2 SINE indel) in the 3' UTR of Comt (catechol-O-methyltransferase).
Title: A transposon in Comt generates mRNA variants and causes widespread expression and behavioral differences among mice.

The following Nsg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nsg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07206	NM_001359092.1 Latest version!	Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu07206	NM_001359091.1 Latest version!	Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu07206	NM_001359090.1 Latest version!	Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu07206	NM_010942.4 Latest version!	Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu07206	XM_006503773.1 Latest version!	Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu07206	NM_010942.3	Mus musculus neuron specific gene family member 1 (Nsg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nsg1 ( NM_010942.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035072.2 Mus musculus neuron specific gene family member 1 (Nsg1), mRNA.

Price & Availability↑

CloneID	OMu07206
Clone ID Related Accession (Same CDS sequence)	NM_010942.3 , NM_001359090.1 , NM_010942.4 , NM_001359091.1 , XM_006503773.1 , NM_001359092.1
Accession Version	NM_010942.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-26
Organism	Mus musculus(House mouse)
Product	neuron-specific protein family member 1
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AX775765.1 and BC008272.1. On Oct 16, 2005 this sequence version replaced NM_010942.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK148941.1, AK078054.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAAGT TGGGGAATAA TTTCGCAGAG AAGGGCACCA AGCAGCCACT GCTGGAGGAT 
GGCTTCGACA CCATTCCTTT GATGACGCCC CTCGATGTCA ACCAGCTGCA GTTCCCACCC 
CCAGATAAGG TCGTGGTGAA AACTAAGACT GAATATGAAC CTGATCGCAA AAAAGGAAAA 
GCACGTCCTC CCAAGATAGC CGAGTTCACC GTCAGCATCA CCGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGCTGGT CCTCTTTGCC CTGGCCTTCC TCACCTGTGT CGTCTTCCTG 
GTTGTCTACA AAGTGTACAA GTATGACCGC GCCTGCCCTG ATGGGTTTGT CTTGAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACACGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACTGTCAT AAACCACTAC AACGTGGCCA AGCAGAGCAT CACCCGCTCC 
GTGTCGCCAT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CGGAACAGGA GACCGAAGCT 
GCAGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035072.2
CDS	104..661
Translation	MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPKIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYTEQDSSAREKFYTVINHYNVAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA

Target ORF information:

RefSeq Version	NM_010942.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus neuron specific gene family member 1 (Nsg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010942.3

ORF Insert Sequence:

ATGGTGAAGT TGGGGAATAA TTTCGCAGAG AAGGGCACCA AGCAGCCACT GCTGGAGGAT 
GGCTTCGACA CCATTCCTTT GATGACGCCC CTCGATGTCA ACCAGCTGCA GTTCCCACCC 
CCAGATAAGG TCGTGGTGAA AACTAAGACT GAATATGAAC CTGATCGCAA AAAAGGAAAA 
GCACGTCCTC CCAAGATAGC CGAGTTCACC GTCAGCATCA CCGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGCTGGT CCTCTTTGCC CTGGCCTTCC TCACCTGTGT CGTCTTCCTG 
GTTGTCTACA AAGTGTACAA GTATGACCGC GCCTGCCCTG ATGGGTTTGT CTTGAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACACGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACTGTCAT AAACCACTAC AACGTGGCCA AGCAGAGCAT CACCCGCTCC 
GTGTCGCCAT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CGGAACAGGA GACCGAAGCT 
GCAGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsg1 ( NM_001359090.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346019.1 Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07206
Clone ID Related Accession (Same CDS sequence)	NM_010942.3 , NM_001359090.1 , NM_010942.4 , NM_001359091.1 , XM_006503773.1 , NM_001359092.1
Accession Version	NM_001359090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	neuronal vesicle trafficking-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115931.7. Transcript Variant: This variant (1) represents the longest transcript. All four variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7652917.1141234.1, SRR7345562.4414768.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGT TGGGGAATAA TTTCGCAGAG AAGGGCACCA AGCAGCCACT GCTGGAGGAT 
GGCTTCGACA CCATTCCTTT GATGACGCCC CTCGATGTCA ACCAGCTGCA GTTCCCACCC 
CCAGATAAGG TCGTGGTGAA AACTAAGACT GAATATGAAC CTGATCGCAA AAAAGGAAAA 
GCACGTCCTC CCAAGATAGC CGAGTTCACC GTCAGCATCA CCGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGCTGGT CCTCTTTGCC CTGGCCTTCC TCACCTGTGT CGTCTTCCTG 
GTTGTCTACA AAGTGTACAA GTATGACCGC GCCTGCCCTG ATGGGTTTGT CTTGAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACACGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACTGTCAT AAACCACTAC AACGTGGCCA AGCAGAGCAT CACCCGCTCC 
GTGTCGCCAT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CGGAACAGGA GACCGAAGCT 
GCAGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346019.1
CDS	739..1296
Translation	MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPKIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYTEQDSSAREKFYTVINHYNVAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA

Target ORF information:

RefSeq Version	NM_001359090.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359090.1

ORF Insert Sequence:

ATGGTGAAGT TGGGGAATAA TTTCGCAGAG AAGGGCACCA AGCAGCCACT GCTGGAGGAT 
GGCTTCGACA CCATTCCTTT GATGACGCCC CTCGATGTCA ACCAGCTGCA GTTCCCACCC 
CCAGATAAGG TCGTGGTGAA AACTAAGACT GAATATGAAC CTGATCGCAA AAAAGGAAAA 
GCACGTCCTC CCAAGATAGC CGAGTTCACC GTCAGCATCA CCGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGCTGGT CCTCTTTGCC CTGGCCTTCC TCACCTGTGT CGTCTTCCTG 
GTTGTCTACA AAGTGTACAA GTATGACCGC GCCTGCCCTG ATGGGTTTGT CTTGAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACACGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACTGTCAT AAACCACTAC AACGTGGCCA AGCAGAGCAT CACCCGCTCC 
GTGTCGCCAT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CGGAACAGGA GACCGAAGCT 
GCAGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsg1 ( NM_010942.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035072.2 Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07206
Clone ID Related Accession (Same CDS sequence)	NM_010942.3 , NM_001359090.1 , NM_010942.4 , NM_001359091.1 , XM_006503773.1 , NM_001359092.1
Accession Version	NM_010942.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	neuronal vesicle trafficking-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK076451.1, AK148941.1 and AC115931.7. On Dec 20, 2017 this sequence version replaced NM_010942.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK148941.1, SRR1660813.176513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGT TGGGGAATAA TTTCGCAGAG AAGGGCACCA AGCAGCCACT GCTGGAGGAT 
GGCTTCGACA CCATTCCTTT GATGACGCCC CTCGATGTCA ACCAGCTGCA GTTCCCACCC 
CCAGATAAGG TCGTGGTGAA AACTAAGACT GAATATGAAC CTGATCGCAA AAAAGGAAAA 
GCACGTCCTC CCAAGATAGC CGAGTTCACC GTCAGCATCA CCGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGCTGGT CCTCTTTGCC CTGGCCTTCC TCACCTGTGT CGTCTTCCTG 
GTTGTCTACA AAGTGTACAA GTATGACCGC GCCTGCCCTG ATGGGTTTGT CTTGAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACACGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACTGTCAT AAACCACTAC AACGTGGCCA AGCAGAGCAT CACCCGCTCC 
GTGTCGCCAT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CGGAACAGGA GACCGAAGCT 
GCAGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035072.2
CDS	380..937
Translation	MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPKIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYTEQDSSAREKFYTVINHYNVAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA

Target ORF information:

RefSeq Version	NM_010942.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010942.4

ORF Insert Sequence:

ATGGTGAAGT TGGGGAATAA TTTCGCAGAG AAGGGCACCA AGCAGCCACT GCTGGAGGAT 
GGCTTCGACA CCATTCCTTT GATGACGCCC CTCGATGTCA ACCAGCTGCA GTTCCCACCC 
CCAGATAAGG TCGTGGTGAA AACTAAGACT GAATATGAAC CTGATCGCAA AAAAGGAAAA 
GCACGTCCTC CCAAGATAGC CGAGTTCACC GTCAGCATCA CCGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGCTGGT CCTCTTTGCC CTGGCCTTCC TCACCTGTGT CGTCTTCCTG 
GTTGTCTACA AAGTGTACAA GTATGACCGC GCCTGCCCTG ATGGGTTTGT CTTGAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACACGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACTGTCAT AAACCACTAC AACGTGGCCA AGCAGAGCAT CACCCGCTCC 
GTGTCGCCAT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CGGAACAGGA GACCGAAGCT 
GCAGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsg1 ( NM_001359091.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346020.1 Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu07206
Clone ID Related Accession (Same CDS sequence)	NM_010942.3 , NM_001359090.1 , NM_010942.4 , NM_001359091.1 , XM_006503773.1 , NM_001359092.1
Accession Version	NM_001359091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	neuronal vesicle trafficking-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115931.7. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.222834.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGT TGGGGAATAA TTTCGCAGAG AAGGGCACCA AGCAGCCACT GCTGGAGGAT 
GGCTTCGACA CCATTCCTTT GATGACGCCC CTCGATGTCA ACCAGCTGCA GTTCCCACCC 
CCAGATAAGG TCGTGGTGAA AACTAAGACT GAATATGAAC CTGATCGCAA AAAAGGAAAA 
GCACGTCCTC CCAAGATAGC CGAGTTCACC GTCAGCATCA CCGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGCTGGT CCTCTTTGCC CTGGCCTTCC TCACCTGTGT CGTCTTCCTG 
GTTGTCTACA AAGTGTACAA GTATGACCGC GCCTGCCCTG ATGGGTTTGT CTTGAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACACGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACTGTCAT AAACCACTAC AACGTGGCCA AGCAGAGCAT CACCCGCTCC 
GTGTCGCCAT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CGGAACAGGA GACCGAAGCT 
GCAGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346020.1
CDS	655..1212
Translation	MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPKIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYTEQDSSAREKFYTVINHYNVAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA

Target ORF information:

RefSeq Version	NM_001359091.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359091.1

ORF Insert Sequence:

ATGGTGAAGT TGGGGAATAA TTTCGCAGAG AAGGGCACCA AGCAGCCACT GCTGGAGGAT 
GGCTTCGACA CCATTCCTTT GATGACGCCC CTCGATGTCA ACCAGCTGCA GTTCCCACCC 
CCAGATAAGG TCGTGGTGAA AACTAAGACT GAATATGAAC CTGATCGCAA AAAAGGAAAA 
GCACGTCCTC CCAAGATAGC CGAGTTCACC GTCAGCATCA CCGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGCTGGT CCTCTTTGCC CTGGCCTTCC TCACCTGTGT CGTCTTCCTG 
GTTGTCTACA AAGTGTACAA GTATGACCGC GCCTGCCCTG ATGGGTTTGT CTTGAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACACGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACTGTCAT AAACCACTAC AACGTGGCCA AGCAGAGCAT CACCCGCTCC 
GTGTCGCCAT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CGGAACAGGA GACCGAAGCT 
GCAGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsg1 ( NM_001359092.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346021.1 Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu07206
Clone ID Related Accession (Same CDS sequence)	NM_010942.3 , NM_001359090.1 , NM_010942.4 , NM_001359091.1 , XM_006503773.1 , NM_001359092.1
Accession Version	NM_001359092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	neuronal vesicle trafficking-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115931.7. On Dec 20, 2017 this sequence version replaced XM_006503773.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.741.1, SRR1660817.216473.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGT TGGGGAATAA TTTCGCAGAG AAGGGCACCA AGCAGCCACT GCTGGAGGAT 
GGCTTCGACA CCATTCCTTT GATGACGCCC CTCGATGTCA ACCAGCTGCA GTTCCCACCC 
CCAGATAAGG TCGTGGTGAA AACTAAGACT GAATATGAAC CTGATCGCAA AAAAGGAAAA 
GCACGTCCTC CCAAGATAGC CGAGTTCACC GTCAGCATCA CCGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGCTGGT CCTCTTTGCC CTGGCCTTCC TCACCTGTGT CGTCTTCCTG 
GTTGTCTACA AAGTGTACAA GTATGACCGC GCCTGCCCTG ATGGGTTTGT CTTGAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACACGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACTGTCAT AAACCACTAC AACGTGGCCA AGCAGAGCAT CACCCGCTCC 
GTGTCGCCAT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CGGAACAGGA GACCGAAGCT 
GCAGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346021.1
CDS	472..1029
Translation	MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPKIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYTEQDSSAREKFYTVINHYNVAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA

Target ORF information:

RefSeq Version	NM_001359092.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359092.1

ORF Insert Sequence:

ATGGTGAAGT TGGGGAATAA TTTCGCAGAG AAGGGCACCA AGCAGCCACT GCTGGAGGAT 
GGCTTCGACA CCATTCCTTT GATGACGCCC CTCGATGTCA ACCAGCTGCA GTTCCCACCC 
CCAGATAAGG TCGTGGTGAA AACTAAGACT GAATATGAAC CTGATCGCAA AAAAGGAAAA 
GCACGTCCTC CCAAGATAGC CGAGTTCACC GTCAGCATCA CCGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGCTGGT CCTCTTTGCC CTGGCCTTCC TCACCTGTGT CGTCTTCCTG 
GTTGTCTACA AAGTGTACAA GTATGACCGC GCCTGCCCTG ATGGGTTTGT CTTGAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACACGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACTGTCAT AAACCACTAC AACGTGGCCA AGCAGAGCAT CACCCGCTCC 
GTGTCGCCAT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CGGAACAGGA GACCGAAGCT 
GCAGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsg1 ( XM_006503773.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503836.1 Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant X1, mRNA.

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CloneID	OMu07206
Clone ID Related Accession (Same CDS sequence)	NM_010942.3 , NM_001359090.1 , NM_010942.4 , NM_001359091.1 , XM_006503773.1 , NM_001359092.1
Accession Version	XM_006503773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	neuron-specific protein family member 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGT TGGGGAATAA TTTCGCAGAG AAGGGCACCA AGCAGCCACT GCTGGAGGAT 
GGCTTCGACA CCATTCCTTT GATGACGCCC CTCGATGTCA ACCAGCTGCA GTTCCCACCC 
CCAGATAAGG TCGTGGTGAA AACTAAGACT GAATATGAAC CTGATCGCAA AAAAGGAAAA 
GCACGTCCTC CCAAGATAGC CGAGTTCACC GTCAGCATCA CCGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGCTGGT CCTCTTTGCC CTGGCCTTCC TCACCTGTGT CGTCTTCCTG 
GTTGTCTACA AAGTGTACAA GTATGACCGC GCCTGCCCTG ATGGGTTTGT CTTGAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACACGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACTGTCAT AAACCACTAC AACGTGGCCA AGCAGAGCAT CACCCGCTCC 
GTGTCGCCAT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CGGAACAGGA GACCGAAGCT 
GCAGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503836.1
CDS	134..691
Translation	MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPKIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYTEQDSSAREKFYTVINHYNVAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA

Target ORF information:

RefSeq Version	XM_006503773.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus neuron specific gene family member 1 (Nsg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503773.1

ORF Insert Sequence:

ATGGTGAAGT TGGGGAATAA TTTCGCAGAG AAGGGCACCA AGCAGCCACT GCTGGAGGAT 
GGCTTCGACA CCATTCCTTT GATGACGCCC CTCGATGTCA ACCAGCTGCA GTTCCCACCC 
CCAGATAAGG TCGTGGTGAA AACTAAGACT GAATATGAAC CTGATCGCAA AAAAGGAAAA 
GCACGTCCTC CCAAGATAGC CGAGTTCACC GTCAGCATCA CCGAGGGTGT CACCGAGAGG 
TTTAAGGTCT CCGTGCTGGT CCTCTTTGCC CTGGCCTTCC TCACCTGTGT CGTCTTCCTG 
GTTGTCTACA AAGTGTACAA GTATGACCGC GCCTGCCCTG ATGGGTTTGT CTTGAAGAAC 
ACCCAGTGCA TCCCAGAAGG CTTGGAGAGC TACTACACGG AGCAAGACTC CAGTGCCCGG 
GAGAAATTTT ACACTGTCAT AAACCACTAC AACGTGGCCA AGCAGAGCAT CACCCGCTCC 
GTGTCGCCAT GGATGTCAGT TCTGTCAGAA GAGAAGCTGT CGGAACAGGA GACCGAAGCT 
GCAGAGAAGT CAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Development (Cambridge, England)144(17)3177-3188(2017 09)
Rutledge EA,Benazet JD,McMahon AP

The endosomal neuronal proteins Nsg1/NEEP21 and Nsg2/P19 are itinerant, not resident proteins of dendritic endosomes.
Scientific reports7(1)10481(2017 Sep)
Yap CC,Digilio L,McMahon L,Winckler B

The related neuronal endosomal proteins NEEP21 (Nsg1) and P19 (Nsg2) have divergent expression profiles in vivo.
The Journal of comparative neurology525(8)1861-1878(2017 Jun)
Barford K,Yap CC,Dwyer ND,Winckler B

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

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Nsg1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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