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PPARA cDNA ORF clone, Mus musculus(House mouse)

Gene Symbol PPARA
Entrez Gene ID 19013
Full Name peroxisome proliferator activated receptor alpha
Synonyms 4933429D07Rik,AW742785,Nr1c1,PPAR-alpha,PPARalpha,Ppar
General protein information
Preferred Names

peroxisome proliferator activated receptor alpha

Names

peroxisome proliferator-activated receptor alpha
nuclear receptor subfamily 1 group C member 1

Gene Type protein-coding
Organism Mus musculus(House mouse)
Genome

15

15 E2|15 40.42 cM


mRNA and Protein(s)

mRNA Protein Name
NM_001113418.1 NP_001106889.1 peroxisome proliferator-activated receptor alpha
XM_011245519.2 XP_011243821.1 peroxisome proliferator-activated receptor alpha isoform X1
XM_006520621.3 XP_006520684.1 peroxisome proliferator-activated receptor alpha isoform X1
XM_011245516.2 XP_011243818.1 peroxisome proliferator-activated receptor alpha isoform X1
XM_006520620.3 XP_006520683.1 peroxisome proliferator-activated receptor alpha isoform X1
XM_006520622.3 XP_006520685.1 peroxisome proliferator-activated receptor alpha isoform X1
NM_011144.6 NP_035274.2 peroxisome proliferator-activated receptor alpha
XM_006520619.1 XP_006520682.1 peroxisome proliferator-activated receptor alpha isoform X1
XM_011245517.2 XP_011243819.1 peroxisome proliferator-activated receptor alpha isoform X1
XM_006520623.3 XP_006520686.1 peroxisome proliferator-activated receptor alpha isoform X1
XM_006520624.3 XP_006520687.1 peroxisome proliferator-activated receptor alpha isoform X2



Bos taurus (cattle) PPARA NP_001029208.1
Mus musculus (house mouse) Ppara NP_001106889.1
Danio rerio (zebrafish) LOC100534818 XP_003201422.1
Pan troglodytes (chimpanzee) PPARA XP_001136470.1
Canis lupus familiaris (dog) PPARA NP_001003093.1
Rattus norvegicus (Norway rat) Ppara NP_037328.1
Danio rerio (zebrafish) pparab NP_001096037.1
Macaca mulatta (rhesus monkey) PPARA NP_001028201.1
Homo sapiens (human) PPARA NP_001001928.1
Xenopus tropicalis (tropical clawed frog) LOC100497346 XP_002940784.2
Gallus gallus (chicken) PPARA NP_001001464.1


Related articles in PubMed

Peroxisome proliferator-activated receptor α (PPARα) contributes to control of melanogenesis in B16 F10 melanoma cells.
Grabacka M, Wieczorek J, Michalczyk-Wetula D, Malinowski M, Wolan N, Wojcik K, Plonka PM
Archives of dermatological research309(3)141-157(2017 Apr)

Inhibition of adipose tissue PPARγ prevents increased adipocyte expansion after lipectomy and exacerbates a glucose-intolerant phenotype.
Booth AD, Magnuson AM, Cox-York KA, Wei Y, Wang D, Pagliassotti MJ, Foster MT
Cell proliferation50(2)(2017 Apr)

Global Deletion of Glutathione S-Transferase A4 Exacerbates Developmental Nonalcoholic Steatohepatitis.
Ronis M, Mercer K, Engi B, Pulliam C, Zimniak P, Hennings L, Shearn C, Badger T, Petersen D
The American journal of pathology187(2)418-430(2017 Feb)

Peroxisome proliferator-activated receptor-α accelerates α-chlorofatty acid catabolism.
Palladino EN, Wang WY, Albert CJ, Langhi C, Baldán Á, Ford DA
Journal of lipid research58(2)317-324(2017 Feb)

Chronic activation of PPARα with fenofibrate reduces autophagic proteins in the liver of mice independent of FGF21.
Jo E, Li S, Liang Q, Zhang X, Wang H, Herbert TP, Jenkins TA, Xu A, Ye JM
PloS one12(4)e0173676(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data raise the possibility that ATGL deficiency could impair the renal fatty acid metabolism though inhibiting PPARalphaexpression, which may lead to lipid deposition and cell apoptosis of PCT, and finally contribute to the renal fibrosis and dysfunction
Title: Atgl gene deletion predisposes to proximal tubule damage by impairing the fatty acid metabolism.

The results indicate that alterations in PGC-1a acetylation and expression level might contribute to the regulation of melanogenesis by PPARalpha and fenofibrate.
Title: Peroxisome proliferator-activated receptor α (PPARα) contributes to control of melanogenesis in B16 F10 melanoma cells.

CYP2A5 protects against the development of alcoholic fatty liver disease, and the PPARalpha-FGF21 axis contributes to the protective effects of CYP2A5 on alcoholic fatty liver disease.
Title: Alcoholic fatty liver is enhanced in CYP2A5 knockout mice: The role of the PPARα-FGF21 axis.

These studies conclusively show that cell proliferation is mediated exclusively by PPARA activation in hepatocytes and that Kupffer cell PPARA has an important role in mediating the anti-inflammatory effects of PPARA agonists.
Title: Hepatocyte-specific PPARA expression exclusively promotes agonist-induced cell proliferation without influence from nonparenchymal cells.

Chronic stimulation of PPARalpha may suppress the autophagy capacity in the liver as a result of reduced content of a number of autophagy-associated proteins independent of FGF21.
Title: Chronic activation of PPARα with fenofibrate reduces autophagic proteins in the liver of mice independent of FGF21.

The following PPARA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPARA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OMu22504 NM_001113418.1
Latest version!
Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu22504 NM_011144.6
Latest version!
Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu22504 XM_011245517.2
Latest version!
Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu22504 XM_006520623.3
Latest version!
Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu22504 XM_006520620.3
Latest version!
Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu22504 XM_006520621.3
Latest version!
Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu22504 XM_011245519.2
Latest version!
Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu22504 XM_006520619.1
Latest version!
Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu22504 XM_011245516.2
Latest version!
Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu22504 XM_006520622.3
Latest version!
Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu51072 XM_006520624.3
Latest version!
Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu22504
Clone ID Related Accession (Same CDS sequence) NM_001113418.1 , XM_011245519.2 , XM_006520621.3 , XM_011245516.2 , XM_006520620.3 , XM_006520622.3 , NM_011144.6 , XM_006520619.1 , XM_011245517.2 , XM_006520623.3
Accession Version NM_001113418.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Mus musculus(House mouse)
Product peroxisome proliferator-activated receptor alpha
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK081709.1, AC117784.13 and AI118064.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK081709.1, AK149460.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001106889.1
CDS208..1614
Misc Feature(1)121..123
Misc Feature(2)508..759
Misc Feature(3)order(511..513,520..522,562..564,571..573,619..621,634..636,664..666,673..675)
Misc Feature(4)order(535..549,565..570,574..576,580..582,586..591,598..600,655..660,667..669,676..678,709..711,718..732,736..738)
Misc Feature(5)order(649..654,658..663)
Misc Feature(6)808..1608
Misc Feature(7)order(1030..1035,1045..1047,1147..1149,1159..1161,1168..1170,1195..1197,1201..1203,1270..1272,1525..1527)
Misc Feature(8)1045..1611
Misc Feature(9)order(1060..1062,1069..1071,1081..1083,1096..1098,1111..1116,1120..1125,1132..1137)
Misc Feature(10)1117..1506
Misc Feature(11)order(1297..1299,1363..1365,1372..1374,1399..1401,1468..1470,1480..1482,1489..1491,1495..1500,1507..1512,1531..1533)
Misc Feature(12)1504..1506
Exon (1)1..83
Exon (2)84..168
Exon (3)169..415
Exon (4)416..576
Exon (5)577..715
Exon (6)716..918
Exon (7)919..1366
Exon (8)1367..7207
Translation

Target ORF information:

RefSeq Version NM_001113418.1
Organism Mus musculus(House mouse)
Definition Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001113418.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22504
Clone ID Related Accession (Same CDS sequence) NM_001113418.1 , XM_011245519.2 , XM_006520621.3 , XM_011245516.2 , XM_006520620.3 , XM_006520622.3 , NM_011144.6 , XM_006520619.1 , XM_011245517.2 , XM_006520623.3
Accession Version NM_011144.6 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Mus musculus(House mouse)
Product peroxisome proliferator-activated receptor alpha
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK035676.1, AC117784.13 and AI118064.1. On Jan 12, 2008 this sequence version replaced NM_011144.5. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK035676.1, BC016892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_035274.2
CDS446..1852
Misc Feature(1)359..361
Misc Feature(2)746..997
Misc Feature(3)order(749..751,758..760,800..802,809..811,857..859,872..874,902..904,911..913)
Misc Feature(4)order(773..787,803..808,812..814,818..820,824..829,836..838,893..898,905..907,914..916,947..949,956..970,974..976)
Misc Feature(5)order(887..892,896..901)
Misc Feature(6)1046..1846
Misc Feature(7)order(1268..1273,1283..1285,1385..1387,1397..1399,1406..1408,1433..1435,1439..1441,1508..1510,1763..1765)
Misc Feature(8)1283..1849
Misc Feature(9)order(1298..1300,1307..1309,1319..1321,1334..1336,1349..1354,1358..1363,1370..1375)
Misc Feature(10)1355..1744
Misc Feature(11)order(1535..1537,1601..1603,1610..1612,1637..1639,1706..1708,1718..1720,1727..1729,1733..1738,1745..1750,1769..1771)
Misc Feature(12)1742..1744
Exon (1)1..321
Exon (2)322..406
Exon (3)407..653
Exon (4)654..814
Exon (5)815..953
Exon (6)954..1156
Exon (7)1157..1604
Exon (8)1605..7445
Translation

Target ORF information:

RefSeq Version NM_011144.6
Organism Mus musculus(House mouse)
Definition Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_011144.6

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22504
Clone ID Related Accession (Same CDS sequence) NM_001113418.1 , XM_011245519.2 , XM_006520621.3 , XM_011245516.2 , XM_006520620.3 , XM_006520622.3 , NM_011144.6 , XM_006520619.1 , XM_011245517.2 , XM_006520623.3
Accession Version XM_011245519.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product peroxisome proliferator-activated receptor alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011243821.1
CDS320..1726
Misc Feature(1)620..871
Misc Feature(2)order(623..625,632..634,674..676,683..685,731..733,746..748,776..778,785..787)
Misc Feature(3)order(647..661,677..682,686..688,692..694,698..703,710..712,767..772,779..781,788..790,821..823,830..844,848..850)
Misc Feature(4)order(761..766,770..775)
Misc Feature(5)920..1720
Misc Feature(6)order(1142..1147,1157..1159,1259..1261,1271..1273,1280..1282,1307..1309,1313..1315,1382..1384,1637..1639)
Misc Feature(7)order(1172..1174,1181..1183,1193..1195,1208..1210,1223..1228,1232..1237,1244..1249)
Misc Feature(8)order(1409..1411,1475..1477,1484..1486,1511..1513,1580..1582,1592..1594,1601..1603,1607..1612,1619..1624,1643..1645)
Translation

Target ORF information:

RefSeq Version XM_011245519.2
Organism Mus musculus(House mouse)
Definition Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011245519.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22504
Clone ID Related Accession (Same CDS sequence) NM_001113418.1 , XM_011245519.2 , XM_006520621.3 , XM_011245516.2 , XM_006520620.3 , XM_006520622.3 , NM_011144.6 , XM_006520619.1 , XM_011245517.2 , XM_006520623.3
Accession Version XM_006520621.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product peroxisome proliferator-activated receptor alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520621.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006520684.1
CDS282..1688
Misc Feature(1)582..833
Misc Feature(2)order(585..587,594..596,636..638,645..647,693..695,708..710,738..740,747..749)
Misc Feature(3)order(609..623,639..644,648..650,654..656,660..665,672..674,729..734,741..743,750..752,783..785,792..806,810..812)
Misc Feature(4)order(723..728,732..737)
Misc Feature(5)882..1682
Misc Feature(6)order(1104..1109,1119..1121,1221..1223,1233..1235,1242..1244,1269..1271,1275..1277,1344..1346,1599..1601)
Misc Feature(7)order(1134..1136,1143..1145,1155..1157,1170..1172,1185..1190,1194..1199,1206..1211)
Misc Feature(8)order(1371..1373,1437..1439,1446..1448,1473..1475,1542..1544,1554..1556,1563..1565,1569..1574,1581..1586,1605..1607)
Translation

Target ORF information:

RefSeq Version XM_006520621.3
Organism Mus musculus(House mouse)
Definition Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006520621.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22504
Clone ID Related Accession (Same CDS sequence) NM_001113418.1 , XM_011245519.2 , XM_006520621.3 , XM_011245516.2 , XM_006520620.3 , XM_006520622.3 , NM_011144.6 , XM_006520619.1 , XM_011245517.2 , XM_006520623.3
Accession Version XM_011245516.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product peroxisome proliferator-activated receptor alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245516.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011243818.1
CDS420..1826
Misc Feature(1)720..971
Misc Feature(2)order(723..725,732..734,774..776,783..785,831..833,846..848,876..878,885..887)
Misc Feature(3)order(747..761,777..782,786..788,792..794,798..803,810..812,867..872,879..881,888..890,921..923,930..944,948..950)
Misc Feature(4)order(861..866,870..875)
Misc Feature(5)1020..1820
Misc Feature(6)order(1242..1247,1257..1259,1359..1361,1371..1373,1380..1382,1407..1409,1413..1415,1482..1484,1737..1739)
Misc Feature(7)order(1272..1274,1281..1283,1293..1295,1308..1310,1323..1328,1332..1337,1344..1349)
Misc Feature(8)order(1509..1511,1575..1577,1584..1586,1611..1613,1680..1682,1692..1694,1701..1703,1707..1712,1719..1724,1743..1745)
Translation

Target ORF information:

RefSeq Version XM_011245516.2
Organism Mus musculus(House mouse)
Definition Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011245516.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22504
Clone ID Related Accession (Same CDS sequence) NM_001113418.1 , XM_011245519.2 , XM_006520621.3 , XM_011245516.2 , XM_006520620.3 , XM_006520622.3 , NM_011144.6 , XM_006520619.1 , XM_011245517.2 , XM_006520623.3
Accession Version XM_006520620.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product peroxisome proliferator-activated receptor alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520620.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006520683.1
CDS192..1598
Misc Feature(1)492..743
Misc Feature(2)order(495..497,504..506,546..548,555..557,603..605,618..620,648..650,657..659)
Misc Feature(3)order(519..533,549..554,558..560,564..566,570..575,582..584,639..644,651..653,660..662,693..695,702..716,720..722)
Misc Feature(4)order(633..638,642..647)
Misc Feature(5)792..1592
Misc Feature(6)order(1014..1019,1029..1031,1131..1133,1143..1145,1152..1154,1179..1181,1185..1187,1254..1256,1509..1511)
Misc Feature(7)order(1044..1046,1053..1055,1065..1067,1080..1082,1095..1100,1104..1109,1116..1121)
Misc Feature(8)order(1281..1283,1347..1349,1356..1358,1383..1385,1452..1454,1464..1466,1473..1475,1479..1484,1491..1496,1515..1517)
Translation

Target ORF information:

RefSeq Version XM_006520620.3
Organism Mus musculus(House mouse)
Definition Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006520620.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22504
Clone ID Related Accession (Same CDS sequence) NM_001113418.1 , XM_011245519.2 , XM_006520621.3 , XM_011245516.2 , XM_006520620.3 , XM_006520622.3 , NM_011144.6 , XM_006520619.1 , XM_011245517.2 , XM_006520623.3
Accession Version XM_006520622.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product peroxisome proliferator-activated receptor alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006520685.1
CDS1147..2553
Misc Feature(1)1447..1698
Misc Feature(2)order(1450..1452,1459..1461,1501..1503,1510..1512,1558..1560,1573..1575,1603..1605,1612..1614)
Misc Feature(3)order(1474..1488,1504..1509,1513..1515,1519..1521,1525..1530,1537..1539,1594..1599,1606..1608,1615..1617,1648..1650,1657..1671,1675..1677)
Misc Feature(4)order(1588..1593,1597..1602)
Misc Feature(5)1747..2547
Misc Feature(6)order(1969..1974,1984..1986,2086..2088,2098..2100,2107..2109,2134..2136,2140..2142,2209..2211,2464..2466)
Misc Feature(7)order(1999..2001,2008..2010,2020..2022,2035..2037,2050..2055,2059..2064,2071..2076)
Misc Feature(8)order(2236..2238,2302..2304,2311..2313,2338..2340,2407..2409,2419..2421,2428..2430,2434..2439,2446..2451,2470..2472)
Translation

Target ORF information:

RefSeq Version XM_006520622.3
Organism Mus musculus(House mouse)
Definition Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006520622.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22504
Clone ID Related Accession (Same CDS sequence) NM_001113418.1 , XM_011245519.2 , XM_006520621.3 , XM_011245516.2 , XM_006520620.3 , XM_006520622.3 , NM_011144.6 , XM_006520619.1 , XM_011245517.2 , XM_006520623.3
Accession Version XM_006520619.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product peroxisome proliferator-activated receptor alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006520682.1
CDS213..1619
Misc Feature(1)513..764
Misc Feature(2)order(516..518,525..527,567..569,576..578,624..626,639..641,669..671,678..680)
Misc Feature(3)order(540..554,570..575,579..581,585..587,591..596,603..605,660..665,672..674,681..683,714..716,723..737,741..743)
Misc Feature(4)order(654..659,663..668)
Misc Feature(5)813..1613
Misc Feature(6)order(1035..1040,1050..1052,1152..1154,1164..1166,1173..1175,1200..1202,1206..1208,1275..1277,1530..1532)
Misc Feature(7)order(1065..1067,1074..1076,1086..1088,1101..1103,1116..1121,1125..1130,1137..1142)
Misc Feature(8)order(1302..1304,1368..1370,1377..1379,1404..1406,1473..1475,1485..1487,1494..1496,1500..1505,1512..1517,1536..1538)
Translation

Target ORF information:

RefSeq Version XM_006520619.1
Organism Mus musculus(House mouse)
Definition Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006520619.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22504
Clone ID Related Accession (Same CDS sequence) NM_001113418.1 , XM_011245519.2 , XM_006520621.3 , XM_011245516.2 , XM_006520620.3 , XM_006520622.3 , NM_011144.6 , XM_006520619.1 , XM_011245517.2 , XM_006520623.3
Accession Version XM_011245517.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product peroxisome proliferator-activated receptor alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011243819.1
CDS643..2049
Misc Feature(1)943..1194
Misc Feature(2)order(946..948,955..957,997..999,1006..1008,1054..1056,1069..1071,1099..1101,1108..1110)
Misc Feature(3)order(970..984,1000..1005,1009..1011,1015..1017,1021..1026,1033..1035,1090..1095,1102..1104,1111..1113,1144..1146,1153..1167,1171..1173)
Misc Feature(4)order(1084..1089,1093..1098)
Misc Feature(5)1243..2043
Misc Feature(6)order(1465..1470,1480..1482,1582..1584,1594..1596,1603..1605,1630..1632,1636..1638,1705..1707,1960..1962)
Misc Feature(7)order(1495..1497,1504..1506,1516..1518,1531..1533,1546..1551,1555..1560,1567..1572)
Misc Feature(8)order(1732..1734,1798..1800,1807..1809,1834..1836,1903..1905,1915..1917,1924..1926,1930..1935,1942..1947,1966..1968)
Translation

Target ORF information:

RefSeq Version XM_011245517.2
Organism Mus musculus(House mouse)
Definition Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011245517.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22504
Clone ID Related Accession (Same CDS sequence) NM_001113418.1 , XM_011245519.2 , XM_006520621.3 , XM_011245516.2 , XM_006520620.3 , XM_006520622.3 , NM_011144.6 , XM_006520619.1 , XM_011245517.2 , XM_006520623.3
Accession Version XM_006520623.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product peroxisome proliferator-activated receptor alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520623.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006520686.1
CDS820..2226
Misc Feature(1)1120..1371
Misc Feature(2)order(1123..1125,1132..1134,1174..1176,1183..1185,1231..1233,1246..1248,1276..1278,1285..1287)
Misc Feature(3)order(1147..1161,1177..1182,1186..1188,1192..1194,1198..1203,1210..1212,1267..1272,1279..1281,1288..1290,1321..1323,1330..1344,1348..1350)
Misc Feature(4)order(1261..1266,1270..1275)
Misc Feature(5)1420..2220
Misc Feature(6)order(1642..1647,1657..1659,1759..1761,1771..1773,1780..1782,1807..1809,1813..1815,1882..1884,2137..2139)
Misc Feature(7)order(1672..1674,1681..1683,1693..1695,1708..1710,1723..1728,1732..1737,1744..1749)
Misc Feature(8)order(1909..1911,1975..1977,1984..1986,2011..2013,2080..2082,2092..2094,2101..2103,2107..2112,2119..2124,2143..2145)
Translation

Target ORF information:

RefSeq Version XM_006520623.3
Organism Mus musculus(House mouse)
Definition Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006520623.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGA TCGGCCTGGC CTTCTAAACA TAGGCTACAT TGAGAAGTTG
CAGGAGGGGA TTGTGCACGT GCTTAAGCTC CACCTGCAGA GCAACCATCC AGATGACACC
TTCCTCTTCC CAAAGCTCCT TCAAAAAATG GTGGACCTTC GGCAGCTGGT CACGGAGCAT
GCGCAGCTCG TACAGGTCAT CAAGAAGACC GAGTCCGACG CAGCGCTGCA CCCACTGTTG
CAAGAGATCT ACAGAGACAT GTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu51072
Clone ID Related Accession (Same CDS sequence) XM_006520624.3
Accession Version XM_006520624.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1404bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product peroxisome proliferator-activated receptor alpha isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520624.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGC TCCTTCAAAA AATGGTGGAC CTTCGGCAGC TGGTCACGGA
GCATGCGCAG CTCGTACAGG TCATCAAGAA GACCGAGTCC GACGCAGCGC TGCACCCACT
GTTGCAAGAG ATCTACAGAG ACATGTACTG ATCTTTCCTG AGATGGCAGG CCGTTGCCAC
TGTTCAGGGA CCTCCGAGGC CTGCGGCCCC ATACAGGAGA GCAGGGATTT GCACAGAGGG
CCTCCCTCCT ACGCTTGGGG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006520687.1
CDS420..1823
Misc Feature(1)720..971
Misc Feature(2)order(723..725,732..734,774..776,783..785,831..833,846..848,876..878,885..887)
Misc Feature(3)order(747..761,777..782,786..788,792..794,798..803,810..812,867..872,879..881,888..890,921..923,930..944,948..950)
Misc Feature(4)order(861..866,870..875)
Misc Feature(5)1020..>1577
Misc Feature(6)order(1245..1250,1257..1259,1266..1271,1356..1361,1368..1373,1380..1382,1389..1391,1491..1493)
Misc Feature(7)order(1272..1274,1281..1283,1293..1295,1308..1310,1323..1328,1332..1337,1344..1349)
Translation

Target ORF information:

RefSeq Version XM_006520624.3
Organism Mus musculus(House mouse)
Definition Mus musculus peroxisome proliferator activated receptor alpha (Ppara), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006520624.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGACA CAGAGAGCCC CATCTGTCCT CTCTCCCCAC TGGAGGCAGA TGACCTGGAA 
AGTCCCTTAT CTGAAGAATT CTTACAAGAA ATGGGAAACA TTCAAGAGAT TTCTCAGTCC
ATCGGTGAGG AGAGCTCTGG AAGCTTTGGT TTTGCAGACT ACCAGTACTT AGGAAGCTGT
CCGGGCTCCG AGGGCTCTGT CATCACAGAC ACCCTCTCTC CAGCTTCCAG CCCTTCCTCA
GTCAGCTGCC CCGTGATCCC CGCCAGCACG GACGAGTCCC CCGGCAGTGC CCTGAACATC
GAGTGTCGAA TATGTGGGGA CAAGGCCTCA GGGTACCACT ACGGAGTTCA CGCATGTGAA
GGCTGTAAGG GCTTCTTTCG GCGAACTATT CGGCTGAAGC TGGTGTACGA CAAGTGTGAT
CGGAGCTGCA AGATTCAGAA GAAGAACCGG AACAAATGCC AGTACTGCCG TTTTCACAAG
TGCCTGTCTG TCGGGATGTC ACACAATGCA ATTCGCTTTG GAAGAATGCC AAGATCTGAA
AAAGCAAAAC TGAAAGCAGA AATTCTTACC TGTGAACACG ACCTGAAAGA TTCGGAAACT
GCAGACCTCA AATCTCTGGG CAAGAGAATC CACGAAGCCT ACCTGAAGAA CTTCAACATG
AACAAGGTCA AGGCCCGGGT CATACTCGCG GGAAAGACCA GCAACAACCC GCCTTTTGTC
ATACATGACA TGGAGACCTT GTGTATGGCC GAGAAGACGC TTGTGGCCAA GATGGTGGCC
AACGGCGTCG AAGACAAAGA GGCAGAGGTC CGATTCTTCC ACTGCTGCCA GTGCATGTCC
GTGGAGACCG TCACGGAGCT CACAGAATTT GCCAAGGCTA TCCCAGGCTT TGCAAACTTG
GACTTGAACG ACCAAGTCAC CTTGCTAAAG TACGGTGTGT ATGAAGCCAT CTTCACGATG
CTGTCCTCCT TGATGAACAA AGACGGGATG CTGATCGCGT ACGGCAATGG CTTTATCACA
CGCGAGTTCC TTAAGAACCT GAGGAAGCCG TTCTGTGACA TCATGGAACC CAAGTTTGAC
TTCGCTATGA AGTTCAATGC CTTAGAACTG GATGACAGTG ACATTTCCCT GTTTGTGGCT
GCTATAATTT GCTGTGGAGC TCCTTCAAAA AATGGTGGAC CTTCGGCAGC TGGTCACGGA
GCATGCGCAG CTCGTACAGG TCATCAAGAA GACCGAGTCC GACGCAGCGC TGCACCCACT
GTTGCAAGAG ATCTACAGAG ACATGTACTG ATCTTTCCTG AGATGGCAGG CCGTTGCCAC
TGTTCAGGGA CCTCCGAGGC CTGCGGCCCC ATACAGGAGA GCAGGGATTT GCACAGAGGG
CCTCCCTCCT ACGCTTGGGG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Peroxisome proliferator-activated receptor α (PPARα) contributes to control of melanogenesis in B16 F10 melanoma cells.
Archives of dermatological research309(3)141-157(2017 Apr)
Grabacka M,Wieczorek J,Michalczyk-Wetula D,Malinowski M,Wolan N,Wojcik K,Plonka PM


book

Inhibition of adipose tissue PPARγ prevents increased adipocyte expansion after lipectomy and exacerbates a glucose-intolerant phenotype.
Cell proliferation50(2)(2017 Apr)
Booth AD,Magnuson AM,Cox-York KA,Wei Y,Wang D,Pagliassotti MJ,Foster MT


book

Global Deletion of Glutathione S-Transferase A4 Exacerbates Developmental Nonalcoholic Steatohepatitis.
The American journal of pathology187(2)418-430(2017 Feb)
Ronis M,Mercer K,Engi B,Pulliam C,Zimniak P,Hennings L,Shearn C,Badger T,Petersen D


book

Peroxisome proliferator-activated receptor-α accelerates α-chlorofatty acid catabolism.
Journal of lipid research58(2)317-324(2017 Feb)
Palladino EN,Wang WY,Albert CJ,Langhi C,Baldán Á,Ford DA


book

Chronic activation of PPARα with fenofibrate reduces autophagic proteins in the liver of mice independent of FGF21.
PloS one12(4)e0173676(2017)
Jo E,Li S,Liang Q,Zhang X,Wang H,Herbert TP,Jenkins TA,Xu A,Ye JM


 
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