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SIX3 cDNA ORF clone, Mus musculus(House mouse)

Gene Symbol SIX3
Entrez Gene ID 20473
Full Name sine oculis-related homeobox 3
Synonyms E130112M24Rik,Six3a,Six3alpha,Six3b,Six3beta
General protein information
Preferred Names

sine oculis-related homeobox 3

Names

homeobox protein SIX3
sine oculis homeobox homolog 3
sine oculis-related homeobox 3 homolog

Gene Type protein-coding
Organism Mus musculus(House mouse)
Genome

17

17 E4|17 55.42 cM


mRNA and Protein(s)

mRNA Protein Name
XM_017317367.1 XP_017172856.1 homeobox protein SIX3 isoform X1
NM_011381.4 NP_035511.2 homeobox protein SIX3



Xenopus tropicalis (tropical clawed frog) six3 XP_002944388.2
Canis lupus familiaris (dog) SIX3 XP_538477.4
Mus musculus (house mouse) Six3 NP_035511.2
Rattus norvegicus (Norway rat) Six3 NP_076480.1
Danio rerio (zebrafish) six3a NP_571437.1
Pan troglodytes (chimpanzee) SIX3 XP_525749.4
Bos taurus (cattle) SIX3 NP_001180053.1
Gallus gallus (chicken) SIX3 NP_989695.1
Homo sapiens (human) SIX3 NP_005404.1


Related articles in PubMed

Identification of a conserved cis-regulatory element controlling mid-diencephalic expression of mouse Six3.
Lee B, Yoon J, Tri Lam D, Yoon J, Baek K, Jeong Y
Genesis (New York, N.Y. : 2000)55(3)(2017 Mar)

The Gene Regulatory Network of Lens Induction Is Wired through Meis-Dependent Shadow Enhancers of Pax6.
Antosova B, Smolikova J, Klimova L, Lachova J, Bendova M, Kozmikova I, Machon O, Kozmik Z
PLoS genetics12(12)e1006441(2016 Dec)

Tcf7l1 protects the anterior neural fold from adopting the neural crest fate.
Mašek J, Machoň O, Kořínek V, Taketo MM, Kozmik Z
Development (Cambridge, England)143(12)2206-16(2016 Jun)

IGF-2/IGF-1R signaling has distinct effects on Sox1, Irx3, and Six3 expressions during ES cell derived-neuroectoderm development in vitro.
Takata N, Sakakura E, Sasai Y
In vitro cellular & developmental biology. Animal52(5)607-15(2016 May)

NOTCH activation interferes with cell fate specification in the gastrulating mouse embryo.
Souilhol C, Perea-Gomez A, Camus A, Beck-Cormier S, Vandormael-Pournin S, Escande M, Collignon J, Cohen-Tannoudji M
Development (Cambridge, England)142(21)3649-60(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SIX3 and SIX6 play distinct but compensatory roles in regulating transcription of gonadotrope-specific genes as gonadotrope cells differentiate.
Title: Homeodomain Proteins SIX3 and SIX6 Regulate Gonadotrope-specific Genes During Pituitary Development.

Sox2 directly activates the Six3 forebrain enhancer.
Title: Genomic code for Sox2 binding uncovers its regulatory role in Six3 activation in the forebrain.

It was shown that Six3 is necessary for ependymal cell maturation during postnatal stages of brain development. In its absence, ependymal cells fail to suppress radial glia characteristics.
Title: Six3 is required for ependymal cell maturation.

Six3OS regulates Six3 activity in developing retina by a mechanism via which promoter-associated long non-coding RNAs can modulate the activity of their associated protein coding genes during development.
Title: The long noncoding RNA Six3OS acts in trans to regulate retinal development by modulating Six3 activity.

Neuroretina specification requires Six3-mediated suppression of Wnt8b a few hours later at the 6- to 8-somite stage.
Title: Neuroretina specification in mouse embryos requires Six3-mediated suppression of Wnt8b in the anterior neural plate.

The following SIX3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIX3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OMu79536 XM_017317367.1
Latest version!
Mus musculus sine oculis-related homeobox 3 (Six3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OMu19606 NM_011381.4
Latest version!
Mus musculus sine oculis-related homeobox 3 (Six3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu79536
Clone ID Related Accession (Same CDS sequence) XM_017317367.1
Accession Version XM_017317367.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1065bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product homeobox protein SIX3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAATATTT ATCCGCCGCT GCTACACGCT CCCGGCTTCT CTTACCTTTC TGCAGGTCAG 
TCCATGGTAT TCCGCTCCCC CCTAGATCTC TATTCCTCCC ACTTCTTGTT GCCAAACTTC
GCCGATTCTC ACCACTGCTC CCTACTTCTG GCGAGTAGCG GCGGCGGGAG CGGTGCGAGC
GGCGGCGGCG GCGGCGCGGG AGGAGGCGGC GGCGGGAACC GTGCGGGAGG CGGCGGTGCT
GGCGGAGCAG GCGGCGGCAG CGGCGGCGGC GGCTCCAGGG CCCCCCCGGA AGAGTTGTCC
ATGTTCCAGT TGCCCACCCT CAACTTCTCG CCGGAGCAGG TGGCCAGCGT CTGCGAGACG
CTGGAGGAGA CGGGCGACAT CGAGCGGCTG GGCCGCTTCC TCTGGTCGCT GCCCGTGGCC
CCCGGGGCGT GCGAGGCCAT CAACAAACAC GAGTCGATCC TGCGCGCGCG CGCTGTCGTC
GCCTTCCACA CCGGCAACTT CCGCGACCTG TACCACATCC TGGAGAACCA CAAGTTCACT
AAGGAGTCTC ACGGCAAGCT GCAAGCCATG TGGCTCGAGG CGCACTACCA GGAGGCCGAG
AAGCTGCGCG GCCGCCCGCT CGGCCCGGTG GACAAGTACC GCGTGCGCAA GAAGTTCCCT
CTGCCGCGCA CCATCTGGGA TGGCGAGCAG AAGACCCATT GCTTCAAGGA GCGGACTCGG
AGCCTGCTGC GGGAGTGGTA CCTGCAGGAT CCCTACCCCA ACCCCAGCAA GAAACGCGAA
CTGGCGCAGG CCACCGGCCT CACCCCCACA CAAGTAGGCA ACTGGTTTAA GAACCGGCGA
CAGCGCGACC GCGCAGCGGC GGCCAAGAAC AGGCTCCAGC ATCAGGCCAT CGGGCCGAGC
GGGATGCGCT CGCTGGCCGA GCCCGGCTGC CCCACGCACG GCTCAGCAGA GTCACCGTCC
ACGGCGGCCA GCCCGACCAC CAGTGTGTCC AGCCTGACGG AGCGCGCGGA CACCGGCACT
TCGATCCTCT CGGTAACCTC CAGCGACTCG GAATGTGATG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017172856.1
CDS52..1116
Translation

Target ORF information:

RefSeq Version XM_017317367.1
Organism Mus musculus(House mouse)
Definition Mus musculus sine oculis-related homeobox 3 (Six3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017317367.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAATATTT ATCCGCCGCT GCTACACGCT CCCGGCTTCT CTTACCTTTC TGCAGGTCAG 
TCCATGGTAT TCCGCTCCCC CCTAGATCTC TATTCCTCCC ACTTCTTGTT GCCAAACTTC
GCCGATTCTC ACCACTGCTC CCTACTTCTG GCGAGTAGCG GCGGCGGGAG CGGTGCGAGC
GGCGGCGGCG GCGGCGCGGG AGGAGGCGGC GGCGGGAACC GTGCGGGAGG CGGCGGTGCT
GGCGGAGCAG GCGGCGGCAG CGGCGGCGGC GGCTCCAGGG CCCCCCCGGA AGAGTTGTCC
ATGTTCCAGT TGCCCACCCT CAACTTCTCG CCGGAGCAGG TGGCCAGCGT CTGCGAGACG
CTGGAGGAGA CGGGCGACAT CGAGCGGCTG GGCCGCTTCC TCTGGTCGCT GCCCGTGGCC
CCCGGGGCGT GCGAGGCCAT CAACAAACAC GAGTCGATCC TGCGCGCGCG CGCTGTCGTC
GCCTTCCACA CCGGCAACTT CCGCGACCTG TACCACATCC TGGAGAACCA CAAGTTCACT
AAGGAGTCTC ACGGCAAGCT GCAAGCCATG TGGCTCGAGG CGCACTACCA GGAGGCCGAG
AAGCTGCGCG GCCGCCCGCT CGGCCCGGTG GACAAGTACC GCGTGCGCAA GAAGTTCCCT
CTGCCGCGCA CCATCTGGGA TGGCGAGCAG AAGACCCATT GCTTCAAGGA GCGGACTCGG
AGCCTGCTGC GGGAGTGGTA CCTGCAGGAT CCCTACCCCA ACCCCAGCAA GAAACGCGAA
CTGGCGCAGG CCACCGGCCT CACCCCCACA CAAGTAGGCA ACTGGTTTAA GAACCGGCGA
CAGCGCGACC GCGCAGCGGC GGCCAAGAAC AGGCTCCAGC ATCAGGCCAT CGGGCCGAGC
GGGATGCGCT CGCTGGCCGA GCCCGGCTGC CCCACGCACG GCTCAGCAGA GTCACCGTCC
ACGGCGGCCA GCCCGACCAC CAGTGTGTCC AGCCTGACGG AGCGCGCGGA CACCGGCACT
TCGATCCTCT CGGTAACCTC CAGCGACTCG GAATGTGATG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19606
Clone ID Related Accession (Same CDS sequence) NM_011381.4
Accession Version NM_011381.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1002bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-22
Organism Mus musculus(House mouse)
Product homeobox protein SIX3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CD351258.1, BC098096.1 and AC166821.2. On Sep 17, 2009 this sequence version replaced NM_011381.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098096.1, D83145.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN00849390 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGTATTCC GCTCCCCCCT AGATCTCTAT TCCTCCCACT TCTTGTTGCC AAACTTCGCC 
GATTCTCACC ACTGCTCCCT ACTTCTGGCG AGTAGCGGCG GCGGGAGCGG TGCGAGCGGC
GGCGGCGGCG GCGCGGGAGG AGGCGGCGGC GGGAACCGTG CGGGAGGCGG CGGTGCTGGC
GGAGCAGGCG GCGGCAGCGG CGGCGGCGGC TCCAGGGCCC CCCCGGAAGA GTTGTCCATG
TTCCAGTTGC CCACCCTCAA CTTCTCGCCG GAGCAGGTGG CCAGCGTCTG CGAGACGCTG
GAGGAGACGG GCGACATCGA GCGGCTGGGC CGCTTCCTCT GGTCGCTGCC CGTGGCCCCC
GGGGCGTGCG AGGCCATCAA CAAACACGAG TCGATCCTGC GCGCGCGCGC TGTCGTCGCC
TTCCACACCG GCAACTTCCG CGACCTGTAC CACATCCTGG AGAACCACAA GTTCACTAAG
GAGTCTCACG GCAAGCTGCA AGCCATGTGG CTCGAGGCGC ACTACCAGGA GGCCGAGAAG
CTGCGCGGCC GCCCGCTCGG CCCGGTGGAC AAGTACCGCG TGCGCAAGAA GTTCCCTCTG
CCGCGCACCA TCTGGGATGG CGAGCAGAAG ACCCATTGCT TCAAGGAGCG GACTCGGAGC
CTGCTGCGGG AGTGGTACCT GCAGGATCCC TACCCCAACC CCAGCAAGAA ACGCGAACTG
GCGCAGGCCA CCGGCCTCAC CCCCACACAA GTAGGCAACT GGTTTAAGAA CCGGCGACAG
CGCGACCGCG CAGCGGCGGC CAAGAACAGG CTCCAGCATC AGGCCATCGG GCCGAGCGGG
ATGCGCTCGC TGGCCGAGCC CGGCTGCCCC ACGCACGGCT CAGCAGAGTC ACCGTCCACG
GCGGCCAGCC CGACCACCAG TGTGTCCAGC CTGACGGAGC GCGCGGACAC CGGCACTTCG
ATCCTCTCGG TAACCTCCAG CGACTCGGAA TGTGATGTAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_035511.2
CDS407..1408
Misc Feature(1)17..19
Misc Feature(2)623..766
Misc Feature(3)668..1009
Misc Feature(4)1031..1177
Misc Feature(5)order(1034..1036,1043..1045,1163..1165,1172..1177)
Misc Feature(6)1103..1111
Exon (1)1..1215
Exon (2)1216..3680
Translation

Target ORF information:

RefSeq Version NM_011381.4
Organism Mus musculus(House mouse)
Definition Mus musculus sine oculis-related homeobox 3 (Six3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_011381.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGTATTCC GCTCCCCCCT AGATCTCTAT TCCTCCCACT TCTTGTTGCC AAACTTCGCC 
GATTCTCACC ACTGCTCCCT ACTTCTGGCG AGTAGCGGCG GCGGGAGCGG TGCGAGCGGC
GGCGGCGGCG GCGCGGGAGG AGGCGGCGGC GGGAACCGTG CGGGAGGCGG CGGTGCTGGC
GGAGCAGGCG GCGGCAGCGG CGGCGGCGGC TCCAGGGCCC CCCCGGAAGA GTTGTCCATG
TTCCAGTTGC CCACCCTCAA CTTCTCGCCG GAGCAGGTGG CCAGCGTCTG CGAGACGCTG
GAGGAGACGG GCGACATCGA GCGGCTGGGC CGCTTCCTCT GGTCGCTGCC CGTGGCCCCC
GGGGCGTGCG AGGCCATCAA CAAACACGAG TCGATCCTGC GCGCGCGCGC TGTCGTCGCC
TTCCACACCG GCAACTTCCG CGACCTGTAC CACATCCTGG AGAACCACAA GTTCACTAAG
GAGTCTCACG GCAAGCTGCA AGCCATGTGG CTCGAGGCGC ACTACCAGGA GGCCGAGAAG
CTGCGCGGCC GCCCGCTCGG CCCGGTGGAC AAGTACCGCG TGCGCAAGAA GTTCCCTCTG
CCGCGCACCA TCTGGGATGG CGAGCAGAAG ACCCATTGCT TCAAGGAGCG GACTCGGAGC
CTGCTGCGGG AGTGGTACCT GCAGGATCCC TACCCCAACC CCAGCAAGAA ACGCGAACTG
GCGCAGGCCA CCGGCCTCAC CCCCACACAA GTAGGCAACT GGTTTAAGAA CCGGCGACAG
CGCGACCGCG CAGCGGCGGC CAAGAACAGG CTCCAGCATC AGGCCATCGG GCCGAGCGGG
ATGCGCTCGC TGGCCGAGCC CGGCTGCCCC ACGCACGGCT CAGCAGAGTC ACCGTCCACG
GCGGCCAGCC CGACCACCAG TGTGTCCAGC CTGACGGAGC GCGCGGACAC CGGCACTTCG
ATCCTCTCGG TAACCTCCAG CGACTCGGAA TGTGATGTAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Identification of a conserved cis-regulatory element controlling mid-diencephalic expression of mouse Six3.
Genesis (New York, N.Y. : 2000)55(3)(2017 Mar)
Lee B,Yoon J,Tri Lam D,Yoon J,Baek K,Jeong Y


book

The Gene Regulatory Network of Lens Induction Is Wired through Meis-Dependent Shadow Enhancers of Pax6.
PLoS genetics12(12)e1006441(2016 Dec)
Antosova B,Smolikova J,Klimova L,Lachova J,Bendova M,Kozmikova I,Machon O,Kozmik Z


book

Tcf7l1 protects the anterior neural fold from adopting the neural crest fate.
Development (Cambridge, England)143(12)2206-16(2016 Jun)
Mašek J,Machoň O,Kořínek V,Taketo MM,Kozmik Z


book

IGF-2/IGF-1R signaling has distinct effects on Sox1, Irx3, and Six3 expressions during ES cell derived-neuroectoderm development in vitro.
In vitro cellular & developmental biology. Animal52(5)607-15(2016 May)
Takata N,Sakakura E,Sasai Y


book

NOTCH activation interferes with cell fate specification in the gastrulating mouse embryo.
Development (Cambridge, England)142(21)3649-60(2015 Nov)
Souilhol C,Perea-Gomez A,Camus A,Beck-Cormier S,Vandormael-Pournin S,Escande M,Collignon J,Cohen-Tannoudji M


 
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