Slc12a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Slc12a2
Entrez Gene ID	20496
Full Name	solute carrier family 12, member 2
Synonyms	9330166H04Rik,Nkcc1,mBSC2,sy-ns
General protein information	Preferred Names solute carrier family 12, member 2 Names solute carrier family 12 member 2 basolateral Na-K-Cl symporter bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 sodium/potassium/chloride cotransporters sodium/potassium/chloride transporters
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 D3\|18 32.15 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009194.3	NP_033220.2	solute carrier family 12 member 2
XM_006525732.3	XP_006525795.1	solute carrier family 12 member 2 isoform X1
XM_006525732.2	XP_006525795.1	solute carrier family 12 member 2 isoform X1

Pathways from BioSystems

Homologies

	SLC12A2	XP_001098624.1
Rattus norvegicus (Norway rat)	Slc12a2	NP_113986.1
Danio rerio (zebrafish)	slc12a2	NP_001002080.1
Drosophila melanogaster (fruit fly)	Ncc69	NP_648572.1
Homo sapiens (human)	SLC12A2	NP_001243390.1
Bos taurus (cattle)	SLC12A2	NP_777207.1
Gallus gallus (chicken)	SLC12A2	XP_004949435.1
Pan troglodytes (chimpanzee)	SLC12A2	XP_003310846.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001557	XP_321556.3
Xenopus tropicalis (tropical clawed frog)	slc12a2	XP_004910531.1
Canis lupus familiaris (dog)	SLC12A2	XP_538611.5
Mus musculus (house mouse)	Slc12a2	NP_033220.2

Gene Ontology

The following Slc12a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc12a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu54442	XM_006525732.3 Latest version!	Mus musculus solute carrier family 12, member 2 (Slc12a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu19154	NM_009194.3 Latest version!	Mus musculus solute carrier family 12, member 2 (Slc12a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OMu54442	XM_006525732.2	Mus musculus solute carrier family 12, member 2 (Slc12a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc12a2 ( XM_006525732.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525795.1 Mus musculus solute carrier family 12, member 2 (Slc12a2), transcript variant X1, mRNA.

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CloneID	OMu54442
Clone ID Related Accession (Same CDS sequence)	XM_006525732.3 , XM_006525732.2
Accession Version	XM_006525732.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525732.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG GGCCCGCGGC GCCCTCGTCT GGCGCCCCGC GGCCCGCCCG GGATGGGGAC 
GCGCCGCTGG CTGCCGCCGC CGGGGTGGAC CTGCCCGGCA CGGCCGTGCC CTCCGGCCAA 
GAGGATGCTA CTACGGCTGG GAGGCAGGCT GGCGGCGGGG TGCGCGGAGA GGGAACCCCA 
GCGGCGGGCG ATGGGCTGGG CAGACCTCTG GGGCCCACCC CCAGCCAGAG CCGCTTCCAG 
GTGGACCCGG TTTCCGAGAA CGCCGGGCGG GCCGCGGCGG CTGCGGCGGC GGCGGCGGCG 
GCTGCGGCTG CGGCCGGGGC GGCGGGCAAG GAGACCCCCG CGGCCGGGAA AGCCGGCGGT 
GAGAGCGGCG TGGCCAAGGG CAGCGAGGAA GCCAAGGGCC GCTTCCGCGT GAACTTCGTG 
GACCCGGCTG CCTCGTCGTC GGCGGACGAC AGCCTGTCGG ATGCGGCGGG GGTCGGTGGC 
GACGGGCCCA ACGTGAGCTT CCAGAACGGC GGGGACACGG TGCTGAGCGA GGGCAGCAGC 
CTGCACTCGG GGGGTGGCAG TGGACACCAC CAGCAGTACT ACTATGACAC CCACACCAAC 
ACCTACTACC TGCGCACCTT CGGCCACAAC ACCATGGACG CGGTGCCCAG GATCGACCAC 
TACCGGCACA CGGCCGCGCA GCTGGGCGAG AAGCTGCTCC GGCCCAGCCT GGCAGAGCTC 
CACGATGAGC TGGAAAAGGA ACCTTTTGAG GATGGCTTTG CGAATGGAGA AGAAAGTACT 
CCAACCAGAG ATGCTGTGGT CGCATACACT GCCGAGAGTA AAGGAGTTGT AAAGTTTGGC 
TGGATCAAGG GTGTATTAGT CCGCTGCATG TTAAACATTT GGGGTGTGAT GCTCTTTATT 
AGATTGTCAT GGATCGTGGG TCAAGCTGGG ATAGGTCTCT CTGTCGTCGT AATAGCAATG 
GCCACTGTTG TGACAACTAT CACAGGATTG TCTACTTCAG CAATAGCTAC CAATGGGTTC 
GTGAGAGGAG GAGGAGCGTA CTATTTAATC TCTAGAAGTC TAGGGCCGGA ATTTGGTGGT 
GCAATTGGCT TGATCTTTGC TTTTGCCAAT GCGGTGGCCG TTGCTATGTA TGTTGTCGGA 
TTCGCAGAGA CTGTGGTGGA GCTGCTTAAG GAACATTCCA TACTTATGAT AGATGAAATC 
AATGATATCC GAATTATTGG AGCCATTACA GTCGTGATTC TTCTGGGCAT CTCAGTGGCT 
GGAATGGAGT GGGAAGCAAA GGCTCAGATT GTGCTGCTGG TGATCCTTCT CCTGGCCATC 
GCCGACTTCG TCATAGGGAC GTTTATCTCA CTGGAGAGCA AGAAGCCCAA GGGTTTCTTT 
GGCTATAAAT CTGAAATATT TAATGAGAAC TTTGGGCCGG ATTTTCGAGA AGAAGAGACT 
TTCTTCTCTG TATTTGCCAT CTTTTTTCCT GCTGCAACTG GTATTCTAGC TGGAGCAAAT 
ATCTCAGGTG ATCTTGCAGA TCCTCAGTCA GCCATACCCA AAGGAACACT CTTAGCCATT 
TTGATTACTA CAGTGGTTTA CATAGGAATT GCAGTGTCTG TGGGTTCGTG TGTTGTTCGG 
GATGCCACTG GCAATGTTAA TGATACCATT ACAACAGAGC TGACAAACTG TACTTCTGCA 
GCCTGCAAAC TAAACTTTGA CTTCTCTTAT TGTGAGAGCA ATACTTGTTC TTACGGGCTA 
ATGAACAACT TTCAGGTGAT GAGCATGGTG TCAGGATTTG CACCCTTGAT CTCTGCAGGG 
ATCTTTTCAG CCACGCTCTC GTCAGCACTA GCATCTCTTG TGAGTGCTCC CAAAATATTT 
CAGGCTCTAT GTAAGGACAA TATCTACCCA GCTTTCCAGA TGTTTGCTAA AGGTTATGGG 
AAAAATAATG AACCGCTTCG TGGTTACATC TTAACATTTC TGATTGCACT GGGATTCATC 
CTGATTGCTG AATTGAACGT TATTGCTCCA ATTATCTCTA ATTTCTTCCT TGCATCGTAT 
GCCTTAATCA ACTTTTCAGT ATTCCATGCA TCACTTGCAA AATCTCCAGG ATGGCGTCCT 
GCATTCAAAT ACTACAACAT GTGGATCTCC CTTATTGGAG CAATTCTTTG TTGCATAGTG 
ATGTTTGTCA TTAACTGGTG GGCTGCGTTG CTCACATATG TGATAGTCCT TGGGCTGTAC 
ATTTATGTTA CTTACAAAAA ACCAGATGTG AACTGGGGAT CCTCCACACA AGCGCTGACT 
TACCTCAGTG CACTGCAGCA TTCAATCCGT CTTTCTGGGG TGGAGGACCA TGTGAAAAAC 
TTCAGACCAC AGTGCCTTGT AATGACAGGT TCTCCAAACT CACGGCCTGC TTTACTTCAT 
CTTGTTCATG ACTTCACGAA AAATGTTGGT TTAATGATCT GTGGCCATGT GCATATGGGC 
CCCAGAAGAC AAGCTATGAA AGAGATGTCC ATTGATCAAG CCAGATATCA GCGATGGCTC 
ATTAAAAACA AAATGAAGGC TTTCTATGCT CCGGTTCACG CAGATGACTT GCGAGAAGGT 
GCACAATACT TGATGCAGGC TGCTGGACTT GGTCGTATGA AGCCAAACAC ACTTGTCCTG 
GGATTTAAGA AAGATTGGTT ACAAGCCGAT ATGAGGGATG TGGATATGTA TATAAACTTA 
TTTCATGATG CTTTTGACAT ACAATTTGGA GTTGTGGTTA TCCGCCTGAA GGAAGGACTG 
GATATATCAC ACCTCCAAGG TCAGGAAGAA TTATTATCTT CACAAGAAAA ATCACCTGGT 
ACCAAGGATG TGGTAGTAAA TGTGGATTAC AGTAAAAAGT CAGATCAAGA TACTTGCAAA 
TCATCTGGTG AAAAATCCAT TACACAGAAA GAATCCAAAG GCCCTATTGT GCCTTTAAAT 
GTAGCTGACC AAAAGCTTCT TGAAGCTAGC ACACAGTTTC AGAAAAAACA AGGGAAGAAT 
ACTATAGATG TCTGGTGGCT TTTTGATGAT GGAGGTTTGA CATTGTTGAT ACCCTATCTT 
CTGACTACCA AGAAAAAGTG GAAAGATTGT AAGATCCGAG TATTCATTGG TGGAAAAATA 
AACAGAATAG ACCATGACCG GAGAGCGATG GCTACTTTAT TGAGTAAATT CCGGATAGAC 
TTCTCTGATA TCATGGTCCT TGGAGATATC AACACCAAGC CAAAAAAAGA AAATATTATT 
GCTTTTGATG ACATGATCGA GCCGTACAGA CTTCACGAAG ATGATAAAGA ACAGGACATT 
GCAGATAAGA TGAAAGAAGA TGAGCCTTGG CGAATCACAG ATAATGAGCT TGAGCTTTAT 
AAGACTAAGA CATATCGACA GATCAGGTTA AATGAATTAT TAAAGGAGCA TTCAAGCACA 
GCTAACATTA TTGTCATGAG TCTTCCAGTC GCACGGAAAG GCGCTGTGTC CAGTGCTCTG 
TATATGGCTT GGTTAGAAGC TCTCTCGAAG GACCTGCCAC CAGTCCTCCT GGTTCGTGGG 
AATCATCAGA GCGTCCTTAC CTTCTACTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525795.1
CDS	296..3868
Translation	MEPGPAAPSSGAPRPARDGDAPLAAAAGVDLPGTAVPSGQEDATTAGRQAGGGVRGEGTPAAGDGLGRPLGPTPSQSRFQVDPVSENAGRAAAAAAAAAAAAAAAGAAGKETPAAGKAGGESGVAKGSEEAKGRFRVNFVDPAASSSADDSLSDAAGVGGDGPNVSFQNGGDTVLSEGSSLHSGGGSGHHQQYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFISLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKSITQKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPVLLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	XM_006525732.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 2 (Slc12a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525732.3

ORF Insert Sequence:

ATGGAGCCCG GGCCCGCGGC GCCCTCGTCT GGCGCCCCGC GGCCCGCCCG GGATGGGGAC 
GCGCCGCTGG CTGCCGCCGC CGGGGTGGAC CTGCCCGGCA CGGCCGTGCC CTCCGGCCAA 
GAGGATGCTA CTACGGCTGG GAGGCAGGCT GGCGGCGGGG TGCGCGGAGA GGGAACCCCA 
GCGGCGGGCG ATGGGCTGGG CAGACCTCTG GGGCCCACCC CCAGCCAGAG CCGCTTCCAG 
GTGGACCCGG TTTCCGAGAA CGCCGGGCGG GCCGCGGCGG CTGCGGCGGC GGCGGCGGCG 
GCTGCGGCTG CGGCCGGGGC GGCGGGCAAG GAGACCCCCG CGGCCGGGAA AGCCGGCGGT 
GAGAGCGGCG TGGCCAAGGG CAGCGAGGAA GCCAAGGGCC GCTTCCGCGT GAACTTCGTG 
GACCCGGCTG CCTCGTCGTC GGCGGACGAC AGCCTGTCGG ATGCGGCGGG GGTCGGTGGC 
GACGGGCCCA ACGTGAGCTT CCAGAACGGC GGGGACACGG TGCTGAGCGA GGGCAGCAGC 
CTGCACTCGG GGGGTGGCAG TGGACACCAC CAGCAGTACT ACTATGACAC CCACACCAAC 
ACCTACTACC TGCGCACCTT CGGCCACAAC ACCATGGACG CGGTGCCCAG GATCGACCAC 
TACCGGCACA CGGCCGCGCA GCTGGGCGAG AAGCTGCTCC GGCCCAGCCT GGCAGAGCTC 
CACGATGAGC TGGAAAAGGA ACCTTTTGAG GATGGCTTTG CGAATGGAGA AGAAAGTACT 
CCAACCAGAG ATGCTGTGGT CGCATACACT GCCGAGAGTA AAGGAGTTGT AAAGTTTGGC 
TGGATCAAGG GTGTATTAGT CCGCTGCATG TTAAACATTT GGGGTGTGAT GCTCTTTATT 
AGATTGTCAT GGATCGTGGG TCAAGCTGGG ATAGGTCTCT CTGTCGTCGT AATAGCAATG 
GCCACTGTTG TGACAACTAT CACAGGATTG TCTACTTCAG CAATAGCTAC CAATGGGTTC 
GTGAGAGGAG GAGGAGCGTA CTATTTAATC TCTAGAAGTC TAGGGCCGGA ATTTGGTGGT 
GCAATTGGCT TGATCTTTGC TTTTGCCAAT GCGGTGGCCG TTGCTATGTA TGTTGTCGGA 
TTCGCAGAGA CTGTGGTGGA GCTGCTTAAG GAACATTCCA TACTTATGAT AGATGAAATC 
AATGATATCC GAATTATTGG AGCCATTACA GTCGTGATTC TTCTGGGCAT CTCAGTGGCT 
GGAATGGAGT GGGAAGCAAA GGCTCAGATT GTGCTGCTGG TGATCCTTCT CCTGGCCATC 
GCCGACTTCG TCATAGGGAC GTTTATCTCA CTGGAGAGCA AGAAGCCCAA GGGTTTCTTT 
GGCTATAAAT CTGAAATATT TAATGAGAAC TTTGGGCCGG ATTTTCGAGA AGAAGAGACT 
TTCTTCTCTG TATTTGCCAT CTTTTTTCCT GCTGCAACTG GTATTCTAGC TGGAGCAAAT 
ATCTCAGGTG ATCTTGCAGA TCCTCAGTCA GCCATACCCA AAGGAACACT CTTAGCCATT 
TTGATTACTA CAGTGGTTTA CATAGGAATT GCAGTGTCTG TGGGTTCGTG TGTTGTTCGG 
GATGCCACTG GCAATGTTAA TGATACCATT ACAACAGAGC TGACAAACTG TACTTCTGCA 
GCCTGCAAAC TAAACTTTGA CTTCTCTTAT TGTGAGAGCA ATACTTGTTC TTACGGGCTA 
ATGAACAACT TTCAGGTGAT GAGCATGGTG TCAGGATTTG CACCCTTGAT CTCTGCAGGG 
ATCTTTTCAG CCACGCTCTC GTCAGCACTA GCATCTCTTG TGAGTGCTCC CAAAATATTT 
CAGGCTCTAT GTAAGGACAA TATCTACCCA GCTTTCCAGA TGTTTGCTAA AGGTTATGGG 
AAAAATAATG AACCGCTTCG TGGTTACATC TTAACATTTC TGATTGCACT GGGATTCATC 
CTGATTGCTG AATTGAACGT TATTGCTCCA ATTATCTCTA ATTTCTTCCT TGCATCGTAT 
GCCTTAATCA ACTTTTCAGT ATTCCATGCA TCACTTGCAA AATCTCCAGG ATGGCGTCCT 
GCATTCAAAT ACTACAACAT GTGGATCTCC CTTATTGGAG CAATTCTTTG TTGCATAGTG 
ATGTTTGTCA TTAACTGGTG GGCTGCGTTG CTCACATATG TGATAGTCCT TGGGCTGTAC 
ATTTATGTTA CTTACAAAAA ACCAGATGTG AACTGGGGAT CCTCCACACA AGCGCTGACT 
TACCTCAGTG CACTGCAGCA TTCAATCCGT CTTTCTGGGG TGGAGGACCA TGTGAAAAAC 
TTCAGACCAC AGTGCCTTGT AATGACAGGT TCTCCAAACT CACGGCCTGC TTTACTTCAT 
CTTGTTCATG ACTTCACGAA AAATGTTGGT TTAATGATCT GTGGCCATGT GCATATGGGC 
CCCAGAAGAC AAGCTATGAA AGAGATGTCC ATTGATCAAG CCAGATATCA GCGATGGCTC 
ATTAAAAACA AAATGAAGGC TTTCTATGCT CCGGTTCACG CAGATGACTT GCGAGAAGGT 
GCACAATACT TGATGCAGGC TGCTGGACTT GGTCGTATGA AGCCAAACAC ACTTGTCCTG 
GGATTTAAGA AAGATTGGTT ACAAGCCGAT ATGAGGGATG TGGATATGTA TATAAACTTA 
TTTCATGATG CTTTTGACAT ACAATTTGGA GTTGTGGTTA TCCGCCTGAA GGAAGGACTG 
GATATATCAC ACCTCCAAGG TCAGGAAGAA TTATTATCTT CACAAGAAAA ATCACCTGGT 
ACCAAGGATG TGGTAGTAAA TGTGGATTAC AGTAAAAAGT CAGATCAAGA TACTTGCAAA 
TCATCTGGTG AAAAATCCAT TACACAGAAA GAATCCAAAG GCCCTATTGT GCCTTTAAAT 
GTAGCTGACC AAAAGCTTCT TGAAGCTAGC ACACAGTTTC AGAAAAAACA AGGGAAGAAT 
ACTATAGATG TCTGGTGGCT TTTTGATGAT GGAGGTTTGA CATTGTTGAT ACCCTATCTT 
CTGACTACCA AGAAAAAGTG GAAAGATTGT AAGATCCGAG TATTCATTGG TGGAAAAATA 
AACAGAATAG ACCATGACCG GAGAGCGATG GCTACTTTAT TGAGTAAATT CCGGATAGAC 
TTCTCTGATA TCATGGTCCT TGGAGATATC AACACCAAGC CAAAAAAAGA AAATATTATT 
GCTTTTGATG ACATGATCGA GCCGTACAGA CTTCACGAAG ATGATAAAGA ACAGGACATT 
GCAGATAAGA TGAAAGAAGA TGAGCCTTGG CGAATCACAG ATAATGAGCT TGAGCTTTAT 
AAGACTAAGA CATATCGACA GATCAGGTTA AATGAATTAT TAAAGGAGCA TTCAAGCACA 
GCTAACATTA TTGTCATGAG TCTTCCAGTC GCACGGAAAG GCGCTGTGTC CAGTGCTCTG 
TATATGGCTT GGTTAGAAGC TCTCTCGAAG GACCTGCCAC CAGTCCTCCT GGTTCGTGGG 
AATCATCAGA GCGTCCTTAC CTTCTACTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a2 ( NM_009194.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033220.2 Mus musculus solute carrier family 12, member 2 (Slc12a2), mRNA.

Price & Availability↑

CloneID	OMu19154
Clone ID Related Accession (Same CDS sequence)	NM_009194.3
Accession Version	NM_009194.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ172807.1, U13174.1, CN711123.1 and AC127678.9. On Sep 25, 2008 this sequence version replaced NM_009194.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U13174.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCCG GGCCCGCGGC GCCCTCGTCT GGCGCCCCGC GGCCCGCCCG GGATGGGGAC 
GCGCCGCTGG CTGCCGCCGC CGGGGTGGAC CTGCCCGGCA CGGCCGTGCC CTCCGGCCAA 
GAGGATGCTA CTACGGCTGG GAGGCAGGCT GGCGGCGGGG TGCGCGGAGA GGGAACCCCA 
GCGGCGGGCG ATGGGCTGGG CAGACCTCTG GGGCCCACCC CCAGCCAGAG CCGCTTCCAG 
GTGGACCCGG TTTCCGAGAA CGCCGGGCGG GCCGCGGCGG CTGCGGCGGC GGCGGCGGCG 
GCTGCGGCTG CGGCCGGGGC GGCGGGCAAG GAGACCCCCG CGGCCGGGAA AGCCGGCGGT 
GAGAGCGGCG TGGCCAAGGG CAGCGAGGAA GCCAAGGGCC GCTTCCGCGT GAACTTCGTG 
GACCCGGCTG CCTCGTCGTC GGCGGACGAC AGCCTGTCGG ATGCGGCGGG GGTCGGTGGC 
GACGGGCCCA ACGTGAGCTT CCAGAACGGC GGGGACACGG TGCTGAGCGA GGGCAGCAGC 
CTGCACTCGG GGGGTGGCAG TGGACACCAC CAGCAGTACT ACTATGACAC CCACACCAAC 
ACCTACTACC TGCGCACCTT CGGCCACAAC ACCATGGACG CGGTGCCCAG GATCGACCAC 
TACCGGCACA CGGCCGCGCA GCTGGGCGAG AAGCTGCTCC GGCCCAGCCT GGCAGAGCTC 
CACGATGAGC TGGAAAAGGA ACCTTTTGAG GATGGCTTTG CGAATGGAGA AGAAAGTACT 
CCAACCAGAG ATGCTGTGGT CGCATACACT GCCGAGAGTA AAGGAGTTGT AAAGTTTGGC 
TGGATCAAGG GTGTATTAGT CCGCTGCATG TTAAACATTT GGGGTGTGAT GCTCTTTATT 
AGATTGTCAT GGATCGTGGG TCAAGCTGGG ATAGGTCTCT CTGTCGTCGT AATAGCAATG 
GCCACTGTTG TGACAACTAT CACAGGATTG TCTACTTCAG CAATAGCTAC CAATGGGTTC 
GTGAGAGGAG GAGGAGCGTA CTATTTAATC TCTAGAAGTC TAGGGCCGGA ATTTGGTGGT 
GCAATTGGCT TGATCTTTGC TTTTGCCAAT GCGGTGGCCG TTGCTATGTA TGTTGTCGGA 
TTCGCAGAGA CTGTGGTGGA GCTGCTTAAG GAACATTCCA TACTTATGAT AGATGAAATC 
AATGATATCC GAATTATTGG AGCCATTACA GTCGTGATTC TTCTGGGCAT CTCAGTGGCT 
GGAATGGAGT GGGAAGCAAA GGCTCAGATT GTGCTGCTGG TGATCCTTCT CCTGGCCATC 
GCCGACTTCG TCATAGGGAC GTTTATCTCA CTGGAGAGCA AGAAGCCCAA GGGTTTCTTT 
GGCTATAAAT CTGAAATATT TAATGAGAAC TTTGGGCCGG ATTTTCGAGA AGAAGAGACT 
TTCTTCTCTG TATTTGCCAT CTTTTTTCCT GCTGCAACTG GTATTCTAGC TGGAGCAAAT 
ATCTCAGGTG ATCTTGCAGA TCCTCAGTCA GCCATACCCA AAGGAACACT CTTAGCCATT 
TTGATTACTA CAGTGGTTTA CATAGGAATT GCAGTGTCTG TGGGTTCGTG TGTTGTTCGG 
GATGCCACTG GCAATGTTAA TGATACCATT ACAACAGAGC TGACAAACTG TACTTCTGCA 
GCCTGCAAAC TAAACTTTGA CTTCTCTTAT TGTGAGAGCA ATACTTGTTC TTACGGGCTA 
ATGAACAACT TTCAGGTGAT GAGCATGGTG TCAGGATTTG CACCCTTGAT CTCTGCAGGG 
ATCTTTTCAG CCACGCTCTC GTCAGCACTA GCATCTCTTG TGAGTGCTCC CAAAATATTT 
CAGGCTCTAT GTAAGGACAA TATCTACCCA GCTTTCCAGA TGTTTGCTAA AGGTTATGGG 
AAAAATAATG AACCGCTTCG TGGTTACATC TTAACATTTC TGATTGCACT GGGATTCATC 
CTGATTGCTG AATTGAACGT TATTGCTCCA ATTATCTCTA ATTTCTTCCT TGCATCGTAT 
GCCTTAATCA ACTTTTCAGT ATTCCATGCA TCACTTGCAA AATCTCCAGG ATGGCGTCCT 
GCATTCAAAT ACTACAACAT GTGGATCTCC CTTATTGGAG CAATTCTTTG TTGCATAGTG 
ATGTTTGTCA TTAACTGGTG GGCTGCGTTG CTCACATATG TGATAGTCCT TGGGCTGTAC 
ATTTATGTTA CTTACAAAAA ACCAGATGTG AACTGGGGAT CCTCCACACA AGCGCTGACT 
TACCTCAGTG CACTGCAGCA TTCAATCCGT CTTTCTGGGG TGGAGGACCA TGTGAAAAAC 
TTCAGACCAC AGTGCCTTGT AATGACAGGT TCTCCAAACT CACGGCCTGC TTTACTTCAT 
CTTGTTCATG ACTTCACGAA AAATGTTGGT TTAATGATCT GTGGCCATGT GCATATGGGC 
CCCAGAAGAC AAGCTATGAA AGAGATGTCC ATTGATCAAG CCAGATATCA GCGATGGCTC 
ATTAAAAACA AAATGAAGGC TTTCTATGCT CCGGTTCACG CAGATGACTT GCGAGAAGGT 
GCACAATACT TGATGCAGGC TGCTGGACTT GGTCGTATGA AGCCAAACAC ACTTGTCCTG 
GGATTTAAGA AAGATTGGTT ACAAGCCGAT ATGAGGGATG TGGATATGTA TATAAACTTA 
TTTCATGATG CTTTTGACAT ACAATTTGGA GTTGTGGTTA TCCGCCTGAA GGAAGGACTG 
GATATATCAC ACCTCCAAGG TCAGGAAGAA TTATTATCTT CACAAGAAAA ATCACCTGGT 
ACCAAGGATG TGGTAGTAAA TGTGGATTAC AGTAAAAAGT CAGATCAAGA TACTTGCAAA 
TCATCTGGTG AAAAATCCAT TACACAGAAA GATGAGGAAG AGGATGGCAA GACTCCAACT 
CAGCCACTGT TGAAAAAAGA ATCCAAAGGC CCTATTGTGC CTTTAAATGT AGCTGACCAA 
AAGCTTCTTG AAGCTAGCAC ACAGTTTCAG AAAAAACAAG GGAAGAATAC TATAGATGTC 
TGGTGGCTTT TTGATGATGG AGGTTTGACA TTGTTGATAC CCTATCTTCT GACTACCAAG 
AAAAAGTGGA AAGATTGTAA GATCCGAGTA TTCATTGGTG GAAAAATAAA CAGAATAGAC 
CATGACCGGA GAGCGATGGC TACTTTATTG AGTAAATTCC GGATAGACTT CTCTGATATC 
ATGGTCCTTG GAGATATCAA CACCAAGCCA AAAAAAGAAA ATATTATTGC TTTTGATGAC 
ATGATCGAGC CGTACAGACT TCACGAAGAT GATAAAGAAC AGGACATTGC AGATAAGATG 
AAAGAAGATG AGCCTTGGCG AATCACAGAT AATGAGCTTG AGCTTTATAA GACTAAGACA 
TATCGACAGA TCAGGTTAAA TGAATTATTA AAGGAGCATT CAAGCACAGC TAACATTATT 
GTCATGAGTC TTCCAGTCGC ACGGAAAGGC GCTGTGTCCA GTGCTCTGTA TATGGCTTGG 
TTAGAAGCTC TCTCGAAGGA CCTGCCACCA GTCCTCCTGG TTCGTGGGAA TCATCAGAGC 
GTCCTTACCT TCTACTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033220.2
CDS	130..3750
Translation	MEPGPAAPSSGAPRPARDGDAPLAAAAGVDLPGTAVPSGQEDATTAGRQAGGGVRGEGTPAAGDGLGRPLGPTPSQSRFQVDPVSENAGRAAAAAAAAAAAAAAAGAAGKETPAAGKAGGESGVAKGSEEAKGRFRVNFVDPAASSSADDSLSDAAGVGGDGPNVSFQNGGDTVLSEGSSLHSGGGSGHHQQYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFISLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKSITQKDEEEDGKTPTQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPVLLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	NM_009194.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 2 (Slc12a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009194.3

ORF Insert Sequence:

ATGGAGCCCG GGCCCGCGGC GCCCTCGTCT GGCGCCCCGC GGCCCGCCCG GGATGGGGAC 
GCGCCGCTGG CTGCCGCCGC CGGGGTGGAC CTGCCCGGCA CGGCCGTGCC CTCCGGCCAA 
GAGGATGCTA CTACGGCTGG GAGGCAGGCT GGCGGCGGGG TGCGCGGAGA GGGAACCCCA 
GCGGCGGGCG ATGGGCTGGG CAGACCTCTG GGGCCCACCC CCAGCCAGAG CCGCTTCCAG 
GTGGACCCGG TTTCCGAGAA CGCCGGGCGG GCCGCGGCGG CTGCGGCGGC GGCGGCGGCG 
GCTGCGGCTG CGGCCGGGGC GGCGGGCAAG GAGACCCCCG CGGCCGGGAA AGCCGGCGGT 
GAGAGCGGCG TGGCCAAGGG CAGCGAGGAA GCCAAGGGCC GCTTCCGCGT GAACTTCGTG 
GACCCGGCTG CCTCGTCGTC GGCGGACGAC AGCCTGTCGG ATGCGGCGGG GGTCGGTGGC 
GACGGGCCCA ACGTGAGCTT CCAGAACGGC GGGGACACGG TGCTGAGCGA GGGCAGCAGC 
CTGCACTCGG GGGGTGGCAG TGGACACCAC CAGCAGTACT ACTATGACAC CCACACCAAC 
ACCTACTACC TGCGCACCTT CGGCCACAAC ACCATGGACG CGGTGCCCAG GATCGACCAC 
TACCGGCACA CGGCCGCGCA GCTGGGCGAG AAGCTGCTCC GGCCCAGCCT GGCAGAGCTC 
CACGATGAGC TGGAAAAGGA ACCTTTTGAG GATGGCTTTG CGAATGGAGA AGAAAGTACT 
CCAACCAGAG ATGCTGTGGT CGCATACACT GCCGAGAGTA AAGGAGTTGT AAAGTTTGGC 
TGGATCAAGG GTGTATTAGT CCGCTGCATG TTAAACATTT GGGGTGTGAT GCTCTTTATT 
AGATTGTCAT GGATCGTGGG TCAAGCTGGG ATAGGTCTCT CTGTCGTCGT AATAGCAATG 
GCCACTGTTG TGACAACTAT CACAGGATTG TCTACTTCAG CAATAGCTAC CAATGGGTTC 
GTGAGAGGAG GAGGAGCGTA CTATTTAATC TCTAGAAGTC TAGGGCCGGA ATTTGGTGGT 
GCAATTGGCT TGATCTTTGC TTTTGCCAAT GCGGTGGCCG TTGCTATGTA TGTTGTCGGA 
TTCGCAGAGA CTGTGGTGGA GCTGCTTAAG GAACATTCCA TACTTATGAT AGATGAAATC 
AATGATATCC GAATTATTGG AGCCATTACA GTCGTGATTC TTCTGGGCAT CTCAGTGGCT 
GGAATGGAGT GGGAAGCAAA GGCTCAGATT GTGCTGCTGG TGATCCTTCT CCTGGCCATC 
GCCGACTTCG TCATAGGGAC GTTTATCTCA CTGGAGAGCA AGAAGCCCAA GGGTTTCTTT 
GGCTATAAAT CTGAAATATT TAATGAGAAC TTTGGGCCGG ATTTTCGAGA AGAAGAGACT 
TTCTTCTCTG TATTTGCCAT CTTTTTTCCT GCTGCAACTG GTATTCTAGC TGGAGCAAAT 
ATCTCAGGTG ATCTTGCAGA TCCTCAGTCA GCCATACCCA AAGGAACACT CTTAGCCATT 
TTGATTACTA CAGTGGTTTA CATAGGAATT GCAGTGTCTG TGGGTTCGTG TGTTGTTCGG 
GATGCCACTG GCAATGTTAA TGATACCATT ACAACAGAGC TGACAAACTG TACTTCTGCA 
GCCTGCAAAC TAAACTTTGA CTTCTCTTAT TGTGAGAGCA ATACTTGTTC TTACGGGCTA 
ATGAACAACT TTCAGGTGAT GAGCATGGTG TCAGGATTTG CACCCTTGAT CTCTGCAGGG 
ATCTTTTCAG CCACGCTCTC GTCAGCACTA GCATCTCTTG TGAGTGCTCC CAAAATATTT 
CAGGCTCTAT GTAAGGACAA TATCTACCCA GCTTTCCAGA TGTTTGCTAA AGGTTATGGG 
AAAAATAATG AACCGCTTCG TGGTTACATC TTAACATTTC TGATTGCACT GGGATTCATC 
CTGATTGCTG AATTGAACGT TATTGCTCCA ATTATCTCTA ATTTCTTCCT TGCATCGTAT 
GCCTTAATCA ACTTTTCAGT ATTCCATGCA TCACTTGCAA AATCTCCAGG ATGGCGTCCT 
GCATTCAAAT ACTACAACAT GTGGATCTCC CTTATTGGAG CAATTCTTTG TTGCATAGTG 
ATGTTTGTCA TTAACTGGTG GGCTGCGTTG CTCACATATG TGATAGTCCT TGGGCTGTAC 
ATTTATGTTA CTTACAAAAA ACCAGATGTG AACTGGGGAT CCTCCACACA AGCGCTGACT 
TACCTCAGTG CACTGCAGCA TTCAATCCGT CTTTCTGGGG TGGAGGACCA TGTGAAAAAC 
TTCAGACCAC AGTGCCTTGT AATGACAGGT TCTCCAAACT CACGGCCTGC TTTACTTCAT 
CTTGTTCATG ACTTCACGAA AAATGTTGGT TTAATGATCT GTGGCCATGT GCATATGGGC 
CCCAGAAGAC AAGCTATGAA AGAGATGTCC ATTGATCAAG CCAGATATCA GCGATGGCTC 
ATTAAAAACA AAATGAAGGC TTTCTATGCT CCGGTTCACG CAGATGACTT GCGAGAAGGT 
GCACAATACT TGATGCAGGC TGCTGGACTT GGTCGTATGA AGCCAAACAC ACTTGTCCTG 
GGATTTAAGA AAGATTGGTT ACAAGCCGAT ATGAGGGATG TGGATATGTA TATAAACTTA 
TTTCATGATG CTTTTGACAT ACAATTTGGA GTTGTGGTTA TCCGCCTGAA GGAAGGACTG 
GATATATCAC ACCTCCAAGG TCAGGAAGAA TTATTATCTT CACAAGAAAA ATCACCTGGT 
ACCAAGGATG TGGTAGTAAA TGTGGATTAC AGTAAAAAGT CAGATCAAGA TACTTGCAAA 
TCATCTGGTG AAAAATCCAT TACACAGAAA GATGAGGAAG AGGATGGCAA GACTCCAACT 
CAGCCACTGT TGAAAAAAGA ATCCAAAGGC CCTATTGTGC CTTTAAATGT AGCTGACCAA 
AAGCTTCTTG AAGCTAGCAC ACAGTTTCAG AAAAAACAAG GGAAGAATAC TATAGATGTC 
TGGTGGCTTT TTGATGATGG AGGTTTGACA TTGTTGATAC CCTATCTTCT GACTACCAAG 
AAAAAGTGGA AAGATTGTAA GATCCGAGTA TTCATTGGTG GAAAAATAAA CAGAATAGAC 
CATGACCGGA GAGCGATGGC TACTTTATTG AGTAAATTCC GGATAGACTT CTCTGATATC 
ATGGTCCTTG GAGATATCAA CACCAAGCCA AAAAAAGAAA ATATTATTGC TTTTGATGAC 
ATGATCGAGC CGTACAGACT TCACGAAGAT GATAAAGAAC AGGACATTGC AGATAAGATG 
AAAGAAGATG AGCCTTGGCG AATCACAGAT AATGAGCTTG AGCTTTATAA GACTAAGACA 
TATCGACAGA TCAGGTTAAA TGAATTATTA AAGGAGCATT CAAGCACAGC TAACATTATT 
GTCATGAGTC TTCCAGTCGC ACGGAAAGGC GCTGTGTCCA GTGCTCTGTA TATGGCTTGG 
TTAGAAGCTC TCTCGAAGGA CCTGCCACCA GTCCTCCTGG TTCGTGGGAA TCATCAGAGC 
GTCCTTACCT TCTACTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a2 ( XM_006525732.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525795.1 Mus musculus solute carrier family 12, member 2 (Slc12a2), transcript variant X1, mRNA.

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CloneID	OMu54442
Clone ID Related Accession (Same CDS sequence)	XM_006525732.3 , XM_006525732.2
Accession Version	XM_006525732.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG GGCCCGCGGC GCCCTCGTCT GGCGCCCCGC GGCCCGCCCG GGATGGGGAC 
GCGCCGCTGG CTGCCGCCGC CGGGGTGGAC CTGCCCGGCA CGGCCGTGCC CTCCGGCCAA 
GAGGATGCTA CTACGGCTGG GAGGCAGGCT GGCGGCGGGG TGCGCGGAGA GGGAACCCCA 
GCGGCGGGCG ATGGGCTGGG CAGACCTCTG GGGCCCACCC CCAGCCAGAG CCGCTTCCAG 
GTGGACCCGG TTTCCGAGAA CGCCGGGCGG GCCGCGGCGG CTGCGGCGGC GGCGGCGGCG 
GCTGCGGCTG CGGCCGGGGC GGCGGGCAAG GAGACCCCCG CGGCCGGGAA AGCCGGCGGT 
GAGAGCGGCG TGGCCAAGGG CAGCGAGGAA GCCAAGGGCC GCTTCCGCGT GAACTTCGTG 
GACCCGGCTG CCTCGTCGTC GGCGGACGAC AGCCTGTCGG ATGCGGCGGG GGTCGGTGGC 
GACGGGCCCA ACGTGAGCTT CCAGAACGGC GGGGACACGG TGCTGAGCGA GGGCAGCAGC 
CTGCACTCGG GGGGTGGCAG TGGACACCAC CAGCAGTACT ACTATGACAC CCACACCAAC 
ACCTACTACC TGCGCACCTT CGGCCACAAC ACCATGGACG CGGTGCCCAG GATCGACCAC 
TACCGGCACA CGGCCGCGCA GCTGGGCGAG AAGCTGCTCC GGCCCAGCCT GGCAGAGCTC 
CACGATGAGC TGGAAAAGGA ACCTTTTGAG GATGGCTTTG CGAATGGAGA AGAAAGTACT 
CCAACCAGAG ATGCTGTGGT CGCATACACT GCCGAGAGTA AAGGAGTTGT AAAGTTTGGC 
TGGATCAAGG GTGTATTAGT CCGCTGCATG TTAAACATTT GGGGTGTGAT GCTCTTTATT 
AGATTGTCAT GGATCGTGGG TCAAGCTGGG ATAGGTCTCT CTGTCGTCGT AATAGCAATG 
GCCACTGTTG TGACAACTAT CACAGGATTG TCTACTTCAG CAATAGCTAC CAATGGGTTC 
GTGAGAGGAG GAGGAGCGTA CTATTTAATC TCTAGAAGTC TAGGGCCGGA ATTTGGTGGT 
GCAATTGGCT TGATCTTTGC TTTTGCCAAT GCGGTGGCCG TTGCTATGTA TGTTGTCGGA 
TTCGCAGAGA CTGTGGTGGA GCTGCTTAAG GAACATTCCA TACTTATGAT AGATGAAATC 
AATGATATCC GAATTATTGG AGCCATTACA GTCGTGATTC TTCTGGGCAT CTCAGTGGCT 
GGAATGGAGT GGGAAGCAAA GGCTCAGATT GTGCTGCTGG TGATCCTTCT CCTGGCCATC 
GCCGACTTCG TCATAGGGAC GTTTATCTCA CTGGAGAGCA AGAAGCCCAA GGGTTTCTTT 
GGCTATAAAT CTGAAATATT TAATGAGAAC TTTGGGCCGG ATTTTCGAGA AGAAGAGACT 
TTCTTCTCTG TATTTGCCAT CTTTTTTCCT GCTGCAACTG GTATTCTAGC TGGAGCAAAT 
ATCTCAGGTG ATCTTGCAGA TCCTCAGTCA GCCATACCCA AAGGAACACT CTTAGCCATT 
TTGATTACTA CAGTGGTTTA CATAGGAATT GCAGTGTCTG TGGGTTCGTG TGTTGTTCGG 
GATGCCACTG GCAATGTTAA TGATACCATT ACAACAGAGC TGACAAACTG TACTTCTGCA 
GCCTGCAAAC TAAACTTTGA CTTCTCTTAT TGTGAGAGCA ATACTTGTTC TTACGGGCTA 
ATGAACAACT TTCAGGTGAT GAGCATGGTG TCAGGATTTG CACCCTTGAT CTCTGCAGGG 
ATCTTTTCAG CCACGCTCTC GTCAGCACTA GCATCTCTTG TGAGTGCTCC CAAAATATTT 
CAGGCTCTAT GTAAGGACAA TATCTACCCA GCTTTCCAGA TGTTTGCTAA AGGTTATGGG 
AAAAATAATG AACCGCTTCG TGGTTACATC TTAACATTTC TGATTGCACT GGGATTCATC 
CTGATTGCTG AATTGAACGT TATTGCTCCA ATTATCTCTA ATTTCTTCCT TGCATCGTAT 
GCCTTAATCA ACTTTTCAGT ATTCCATGCA TCACTTGCAA AATCTCCAGG ATGGCGTCCT 
GCATTCAAAT ACTACAACAT GTGGATCTCC CTTATTGGAG CAATTCTTTG TTGCATAGTG 
ATGTTTGTCA TTAACTGGTG GGCTGCGTTG CTCACATATG TGATAGTCCT TGGGCTGTAC 
ATTTATGTTA CTTACAAAAA ACCAGATGTG AACTGGGGAT CCTCCACACA AGCGCTGACT 
TACCTCAGTG CACTGCAGCA TTCAATCCGT CTTTCTGGGG TGGAGGACCA TGTGAAAAAC 
TTCAGACCAC AGTGCCTTGT AATGACAGGT TCTCCAAACT CACGGCCTGC TTTACTTCAT 
CTTGTTCATG ACTTCACGAA AAATGTTGGT TTAATGATCT GTGGCCATGT GCATATGGGC 
CCCAGAAGAC AAGCTATGAA AGAGATGTCC ATTGATCAAG CCAGATATCA GCGATGGCTC 
ATTAAAAACA AAATGAAGGC TTTCTATGCT CCGGTTCACG CAGATGACTT GCGAGAAGGT 
GCACAATACT TGATGCAGGC TGCTGGACTT GGTCGTATGA AGCCAAACAC ACTTGTCCTG 
GGATTTAAGA AAGATTGGTT ACAAGCCGAT ATGAGGGATG TGGATATGTA TATAAACTTA 
TTTCATGATG CTTTTGACAT ACAATTTGGA GTTGTGGTTA TCCGCCTGAA GGAAGGACTG 
GATATATCAC ACCTCCAAGG TCAGGAAGAA TTATTATCTT CACAAGAAAA ATCACCTGGT 
ACCAAGGATG TGGTAGTAAA TGTGGATTAC AGTAAAAAGT CAGATCAAGA TACTTGCAAA 
TCATCTGGTG AAAAATCCAT TACACAGAAA GAATCCAAAG GCCCTATTGT GCCTTTAAAT 
GTAGCTGACC AAAAGCTTCT TGAAGCTAGC ACACAGTTTC AGAAAAAACA AGGGAAGAAT 
ACTATAGATG TCTGGTGGCT TTTTGATGAT GGAGGTTTGA CATTGTTGAT ACCCTATCTT 
CTGACTACCA AGAAAAAGTG GAAAGATTGT AAGATCCGAG TATTCATTGG TGGAAAAATA 
AACAGAATAG ACCATGACCG GAGAGCGATG GCTACTTTAT TGAGTAAATT CCGGATAGAC 
TTCTCTGATA TCATGGTCCT TGGAGATATC AACACCAAGC CAAAAAAAGA AAATATTATT 
GCTTTTGATG ACATGATCGA GCCGTACAGA CTTCACGAAG ATGATAAAGA ACAGGACATT 
GCAGATAAGA TGAAAGAAGA TGAGCCTTGG CGAATCACAG ATAATGAGCT TGAGCTTTAT 
AAGACTAAGA CATATCGACA GATCAGGTTA AATGAATTAT TAAAGGAGCA TTCAAGCACA 
GCTAACATTA TTGTCATGAG TCTTCCAGTC GCACGGAAAG GCGCTGTGTC CAGTGCTCTG 
TATATGGCTT GGTTAGAAGC TCTCTCGAAG GACCTGCCAC CAGTCCTCCT GGTTCGTGGG 
AATCATCAGA GCGTCCTTAC CTTCTACTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525795.1
CDS	296..3868
Translation	MEPGPAAPSSGAPRPARDGDAPLAAAAGVDLPGTAVPSGQEDATTAGRQAGGGVRGEGTPAAGDGLGRPLGPTPSQSRFQVDPVSENAGRAAAAAAAAAAAAAAAGAAGKETPAAGKAGGESGVAKGSEEAKGRFRVNFVDPAASSSADDSLSDAAGVGGDGPNVSFQNGGDTVLSEGSSLHSGGGSGHHQQYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFISLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKSITQKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPVLLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	XM_006525732.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 2 (Slc12a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525732.2

ORF Insert Sequence:

ATGGAGCCCG GGCCCGCGGC GCCCTCGTCT GGCGCCCCGC GGCCCGCCCG GGATGGGGAC 
GCGCCGCTGG CTGCCGCCGC CGGGGTGGAC CTGCCCGGCA CGGCCGTGCC CTCCGGCCAA 
GAGGATGCTA CTACGGCTGG GAGGCAGGCT GGCGGCGGGG TGCGCGGAGA GGGAACCCCA 
GCGGCGGGCG ATGGGCTGGG CAGACCTCTG GGGCCCACCC CCAGCCAGAG CCGCTTCCAG 
GTGGACCCGG TTTCCGAGAA CGCCGGGCGG GCCGCGGCGG CTGCGGCGGC GGCGGCGGCG 
GCTGCGGCTG CGGCCGGGGC GGCGGGCAAG GAGACCCCCG CGGCCGGGAA AGCCGGCGGT 
GAGAGCGGCG TGGCCAAGGG CAGCGAGGAA GCCAAGGGCC GCTTCCGCGT GAACTTCGTG 
GACCCGGCTG CCTCGTCGTC GGCGGACGAC AGCCTGTCGG ATGCGGCGGG GGTCGGTGGC 
GACGGGCCCA ACGTGAGCTT CCAGAACGGC GGGGACACGG TGCTGAGCGA GGGCAGCAGC 
CTGCACTCGG GGGGTGGCAG TGGACACCAC CAGCAGTACT ACTATGACAC CCACACCAAC 
ACCTACTACC TGCGCACCTT CGGCCACAAC ACCATGGACG CGGTGCCCAG GATCGACCAC 
TACCGGCACA CGGCCGCGCA GCTGGGCGAG AAGCTGCTCC GGCCCAGCCT GGCAGAGCTC 
CACGATGAGC TGGAAAAGGA ACCTTTTGAG GATGGCTTTG CGAATGGAGA AGAAAGTACT 
CCAACCAGAG ATGCTGTGGT CGCATACACT GCCGAGAGTA AAGGAGTTGT AAAGTTTGGC 
TGGATCAAGG GTGTATTAGT CCGCTGCATG TTAAACATTT GGGGTGTGAT GCTCTTTATT 
AGATTGTCAT GGATCGTGGG TCAAGCTGGG ATAGGTCTCT CTGTCGTCGT AATAGCAATG 
GCCACTGTTG TGACAACTAT CACAGGATTG TCTACTTCAG CAATAGCTAC CAATGGGTTC 
GTGAGAGGAG GAGGAGCGTA CTATTTAATC TCTAGAAGTC TAGGGCCGGA ATTTGGTGGT 
GCAATTGGCT TGATCTTTGC TTTTGCCAAT GCGGTGGCCG TTGCTATGTA TGTTGTCGGA 
TTCGCAGAGA CTGTGGTGGA GCTGCTTAAG GAACATTCCA TACTTATGAT AGATGAAATC 
AATGATATCC GAATTATTGG AGCCATTACA GTCGTGATTC TTCTGGGCAT CTCAGTGGCT 
GGAATGGAGT GGGAAGCAAA GGCTCAGATT GTGCTGCTGG TGATCCTTCT CCTGGCCATC 
GCCGACTTCG TCATAGGGAC GTTTATCTCA CTGGAGAGCA AGAAGCCCAA GGGTTTCTTT 
GGCTATAAAT CTGAAATATT TAATGAGAAC TTTGGGCCGG ATTTTCGAGA AGAAGAGACT 
TTCTTCTCTG TATTTGCCAT CTTTTTTCCT GCTGCAACTG GTATTCTAGC TGGAGCAAAT 
ATCTCAGGTG ATCTTGCAGA TCCTCAGTCA GCCATACCCA AAGGAACACT CTTAGCCATT 
TTGATTACTA CAGTGGTTTA CATAGGAATT GCAGTGTCTG TGGGTTCGTG TGTTGTTCGG 
GATGCCACTG GCAATGTTAA TGATACCATT ACAACAGAGC TGACAAACTG TACTTCTGCA 
GCCTGCAAAC TAAACTTTGA CTTCTCTTAT TGTGAGAGCA ATACTTGTTC TTACGGGCTA 
ATGAACAACT TTCAGGTGAT GAGCATGGTG TCAGGATTTG CACCCTTGAT CTCTGCAGGG 
ATCTTTTCAG CCACGCTCTC GTCAGCACTA GCATCTCTTG TGAGTGCTCC CAAAATATTT 
CAGGCTCTAT GTAAGGACAA TATCTACCCA GCTTTCCAGA TGTTTGCTAA AGGTTATGGG 
AAAAATAATG AACCGCTTCG TGGTTACATC TTAACATTTC TGATTGCACT GGGATTCATC 
CTGATTGCTG AATTGAACGT TATTGCTCCA ATTATCTCTA ATTTCTTCCT TGCATCGTAT 
GCCTTAATCA ACTTTTCAGT ATTCCATGCA TCACTTGCAA AATCTCCAGG ATGGCGTCCT 
GCATTCAAAT ACTACAACAT GTGGATCTCC CTTATTGGAG CAATTCTTTG TTGCATAGTG 
ATGTTTGTCA TTAACTGGTG GGCTGCGTTG CTCACATATG TGATAGTCCT TGGGCTGTAC 
ATTTATGTTA CTTACAAAAA ACCAGATGTG AACTGGGGAT CCTCCACACA AGCGCTGACT 
TACCTCAGTG CACTGCAGCA TTCAATCCGT CTTTCTGGGG TGGAGGACCA TGTGAAAAAC 
TTCAGACCAC AGTGCCTTGT AATGACAGGT TCTCCAAACT CACGGCCTGC TTTACTTCAT 
CTTGTTCATG ACTTCACGAA AAATGTTGGT TTAATGATCT GTGGCCATGT GCATATGGGC 
CCCAGAAGAC AAGCTATGAA AGAGATGTCC ATTGATCAAG CCAGATATCA GCGATGGCTC 
ATTAAAAACA AAATGAAGGC TTTCTATGCT CCGGTTCACG CAGATGACTT GCGAGAAGGT 
GCACAATACT TGATGCAGGC TGCTGGACTT GGTCGTATGA AGCCAAACAC ACTTGTCCTG 
GGATTTAAGA AAGATTGGTT ACAAGCCGAT ATGAGGGATG TGGATATGTA TATAAACTTA 
TTTCATGATG CTTTTGACAT ACAATTTGGA GTTGTGGTTA TCCGCCTGAA GGAAGGACTG 
GATATATCAC ACCTCCAAGG TCAGGAAGAA TTATTATCTT CACAAGAAAA ATCACCTGGT 
ACCAAGGATG TGGTAGTAAA TGTGGATTAC AGTAAAAAGT CAGATCAAGA TACTTGCAAA 
TCATCTGGTG AAAAATCCAT TACACAGAAA GAATCCAAAG GCCCTATTGT GCCTTTAAAT 
GTAGCTGACC AAAAGCTTCT TGAAGCTAGC ACACAGTTTC AGAAAAAACA AGGGAAGAAT 
ACTATAGATG TCTGGTGGCT TTTTGATGAT GGAGGTTTGA CATTGTTGAT ACCCTATCTT 
CTGACTACCA AGAAAAAGTG GAAAGATTGT AAGATCCGAG TATTCATTGG TGGAAAAATA 
AACAGAATAG ACCATGACCG GAGAGCGATG GCTACTTTAT TGAGTAAATT CCGGATAGAC 
TTCTCTGATA TCATGGTCCT TGGAGATATC AACACCAAGC CAAAAAAAGA AAATATTATT 
GCTTTTGATG ACATGATCGA GCCGTACAGA CTTCACGAAG ATGATAAAGA ACAGGACATT 
GCAGATAAGA TGAAAGAAGA TGAGCCTTGG CGAATCACAG ATAATGAGCT TGAGCTTTAT 
AAGACTAAGA CATATCGACA GATCAGGTTA AATGAATTAT TAAAGGAGCA TTCAAGCACA 
GCTAACATTA TTGTCATGAG TCTTCCAGTC GCACGGAAAG GCGCTGTGTC CAGTGCTCTG 
TATATGGCTT GGTTAGAAGC TCTCTCGAAG GACCTGCCAC CAGTCCTCCT GGTTCGTGGG 
AATCATCAGA GCGTCCTTAC CTTCTACTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Slc12a2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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