Fchsd2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Fchsd2
Entrez Gene ID	207278
Full Name	FCH and double SH3 domains 2
Synonyms	BC034086,R74866,Sh3md3,mKIAA0769
General protein information	Preferred Names FCH and double SH3 domains 2 Names F-BAR and double SH3 domains protein 2 FCH and double SH3 domains protein 2 SH3 multiple domains 3 SH3 multiple domains protein 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_199012.2	NP_950177.2	F-BAR and double SH3 domains protein 2 isoform 1
XM_006507518.2	XP_006507581.1	F-BAR and double SH3 domains protein 2 isoform X2
XM_006507517.3	XP_006507580.1	F-BAR and double SH3 domains protein 2 isoform X1
NM_001146010.1	NP_001139482.1	F-BAR and double SH3 domains protein 2 isoform 2
XM_006507518.3	XP_006507581.1	F-BAR and double SH3 domains protein 2 isoform X3
XM_030242304.1	XP_030098164.1	F-BAR and double SH3 domains protein 2 isoform X2

Pathways from BioSystems

Homologies

	FCHSD2	XP_002799736.1
Rattus norvegicus (Norway rat)	Fchsd2	NP_001101009.1
Pan troglodytes (chimpanzee)	FCHSD2	XP_001174760.3
Bos taurus (cattle)	FCHSD2	XP_002693485.1
Gallus gallus (chicken)	FCHSD2	XP_417258.4
Danio rerio (zebrafish)	LOC100536453	XP_003199428.2
Xenopus tropicalis (tropical clawed frog)	fchsd2	NP_001096157.1
Homo sapiens (human)	FCHSD2	NP_055639.2
Danio rerio (zebrafish)	LOC100536596	XP_003199430.2
Drosophila melanogaster (fruit fly)	nwk	NP_001246692.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006774	XP_308970.4
Canis lupus familiaris (dog)	FCHSD2	XP_005633604.1
Mus musculus (house mouse)	Fchsd2	NP_950177.2

Gene Ontology
Bibliography

Related articles in PubMed

Formation of membrane ridges and scallops by the F-BAR protein Nervous Wreck.
Becalska AN, Kelley CF, Berciu C, Stanishneva-Konovalova TB, Fu X, Wang S, Sokolova OS, Nicastro D, Rodal AA
Molecular biology of the cell24(15)2406-18(2013 Aug)

FCHSD1 and FCHSD2 are expressed in hair cell stereocilia and cuticular plate and regulate actin polymerization in vitro.
Cao H, Yin X, Cao Y, Jin Y, Wang S, Kong Y, Chen Y, Gao J, Heller S, Xu Z
PloS one8(2)e56516(2013)

Telencephalic embryonic subtractive sequences: a unique collection of neurodevelopmental genes.
Bulfone A, Carotenuto P, Faedo A, Aglio V, Garzia L, Bello AM, Basile A, Andr? A, Cocchia M, Guardiola O, Ballabio A, Rubenstein JL, Zollo M
The Journal of neuroscience : the official journal of the Society for Neuroscience25(33)7586-600(2005 Aug)

Identification and characterization of human FCHSD1 and FCHSD2 genes in silico.
Katoh M, Katoh M
International journal of molecular medicine13(5)749-54(2004 May)

Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Aizawa H, Yuasa S, Nakajima D, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(1)35-48(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results suggest that FCHSD1 and FCHSD2 could modulate F-actin assembly or maintenance in hair cell stereocilia and cuticular plate.
Title: FCHSD1 and FCHSD2 are expressed in hair cell stereocilia and cuticular plate and regulate actin polymerization in vitro.

The following Fchsd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fchsd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu39402	XM_006507517.3 Latest version!	Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu07138	NM_001146010.1 Latest version!	Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$433.30 $619.00
OMu09266	NM_199012.2 Latest version!	Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$447.30 $639.00
OMu39403	XM_006507518.3 Latest version!	Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu89407	XM_030242304.1 Latest version!	Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu39403	XM_006507518.2	Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fchsd2 ( XM_006507517.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507580.1 Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu39402
Clone ID Related Accession (Same CDS sequence)	XM_006507517.3
Accession Version	XM_006507517.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	F-BAR and double SH3 domains protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507517.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCTG TTTGGAAATC TTTTCTCGAG GGAACAATGC AGGTAGCCCA GTCTCGGATC 
AATATATGTG AAAACTATAA AAACTTCATT TCTGAACCTG CAAGAGCAGT AAGAAGCTTA 
AAAGAACAGC AACTAAAACG GTGTGTGGAT CAGTTGACAA AGATCCAAAC AGAATTACAG 
GAAACTGTGA AAGATTTAGT GAAAGGCAAA AAGAAGTACT TTGAGACTGA ACAGATGGCT 
CATGCAGTAA GAGAGAAAGC TGACATCGAA GCAAAGTCTA AACTTAGTCT TTTTCAATCA 
AGAATCAGTT TACAGAAAGC AAGTGTGAAG TTAAAAGCCC GGCGATCTGA ATGTAATACC 
AAAGCTACCC ATGCAAGGAA TGATTACCTT CTGACGCTAG CAGCAGCTAA CGCACATCAG 
GACCGCTACT ATCAAACAGA CTTAGTTAAC ATCATGAAGG CTCTTGATGG AAACGTGTAT 
GACCACCTCA AGGATTATTT AATAGCCTTC AGCCGGACCG AGCTAGAAAC TTGCCAAGCT 
ATACAGAATA CATTCCAGTT TTTACTAGAA AACTCCAGCA AGGTGGTACG GGACTATAAC 
CTTCAGCTGT TTCTACAAGA GAATGCTGTA TTTCACAAGC CTCAGCCCTT CCAGTTCCAG 
CCTTGTGACA GTGATACTAG TTTAAAAGCC GCCCAACTGG TGGATATTGA GCTCTCCCCT 
GTCAGTGCTC TGAGAATGAC GATAGCTGAG AGTCGACAAT TAGAATCAGA AACTGGTACC 
ACAGAGGAAC ACAGTCTAAA TAAGGAGGCT CGGAAATGGG CCACACGTGT GGCACGTGAG 
CACAAAAATA TTGTTCATCA GCAGCGGGTT CTAAATGAAC TAGAATGTCA TGGAGTTGCT 
CTATCAGAAC AGAGCCGAGC AGAGCTGGAA CAGAAAATAG ATGAAGCAAG AGAAAGTATC 
CGTAAAGCAG AGATCATTAA ATTGAAAGCT GAAGCCCGGC TAGACCTACT GAAGCAGATT 
GGGGTTTCTG TAGATACGTG GTTAAAGAGT GCCATGAACC AAGTGATGGA GGAACTAGAA 
AATGAGCGGT GGGCCCGTCC TCCAGCCGTG ACCAGCAATG GCACTCTACA CTCGCTTAAT 
GCAGATGCTG AAAGAGAAGA AGGAGAAGAG TTTGAAGACA ACATGGATGT TTTTGATGAC 
AGCAGCTCCA GCCCTTCTGG CACCTTGAGA AATTATCCAC TCACCTGCAA AGTTGTTTAT 
TCCTATAAGG CTTCTCAACC AGATGAGTTG ACCATTGAGG AACATGAGGT GTTAGAAGTG 
ATTGAAGATG GAGATATGGA AGACTGGGTA AAGGCTCGAA ATAAAGTTGG CCAAGTGGGT 
TATGTGCCAG AAAAGTACCT ACAATTTCCC ACCTCGAACA GCCTACTGAG CATGCTACAG 
TCTTTGGCTG CTTTGGACAG TCGGTCACAC ACATCCAGCA ATTCCACAGA AGCAGAACTC 
GTTTCAGGCA GCCTGAATGG AGATGCCAGT GTCTGTTTTG TGAAAGCACT TTATGATTAT 
GAGGGCCAGA CAGATGATGA GCTGTCCTTT CCCGAGGGAG CAATTATTCG CATCTTGAAC 
AAAGAAAACC AAGATGATGA TGGCTTCTGG GAAGGGGAGT TCAGTGGTCG AATCGGTGTT 
TTCCCATCGG TGCTAGTGGA AGAGCTCTCA GCCTCCGAGA ATGGCGACAC TCCATGGACA 
AGAGAGATCC AGATCTCTCC ATCCCCCAAG CCTCACACAT CCCTGCCTCC ACTGCCTCTG 
TATGACCAGC CACCCAGCAG CCCGTATCCC AGTCCAGATA AGAGGAGCTC CCAGTTCTTC 
CCCCGGTCTC CTTCAGCCAA TGAAAACAGC CTTCATGCTG AATCACCAGG ATTCTCACAG 
GCCTCAAGAC AGACTCCTGA CACCTCATAT GGCAAGCTGC GGCCTGTTCG GGCGGCGCCG 
CCACCACCCA CACAGAACCA CCGGCGGACA ACTGAGAAGA TGGAGGACGT GGAGATCACA 
CTGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507580.1
CDS	167..2215
Translation	MYPVWKSFLEGTMQVAQSRINICENYKNFISEPARAVRSLKEQQLKRCVDQLTKIQTELQETVKDLVKGKKKYFETEQMAHAVREKADIEAKSKLSLFQSRISLQKASVKLKARRSECNTKATHARNDYLLTLAAANAHQDRYYQTDLVNIMKALDGNVYDHLKDYLIAFSRTELETCQAIQNTFQFLLENSSKVVRDYNLQLFLQENAVFHKPQPFQFQPCDSDTSLKAAQLVDIELSPVSALRMTIAESRQLESETGTTEEHSLNKEARKWATRVAREHKNIVHQQRVLNELECHGVALSEQSRAELEQKIDEARESIRKAEIIKLKAEARLDLLKQIGVSVDTWLKSAMNQVMEELENERWARPPAVTSNGTLHSLNADAEREEGEEFEDNMDVFDDSSSSPSGTLRNYPLTCKVVYSYKASQPDELTIEEHEVLEVIEDGDMEDWVKARNKVGQVGYVPEKYLQFPTSNSLLSMLQSLAALDSRSHTSSNSTEAELVSGSLNGDASVCFVKALYDYEGQTDDELSFPEGAIIRILNKENQDDDGFWEGEFSGRIGVFPSVLVEELSASENGDTPWTREIQISPSPKPHTSLPPLPLYDQPPSSPYPSPDKRSSQFFPRSPSANENSLHAESPGFSQASRQTPDTSYGKLRPVRAAPPPPTQNHRRTTEKMEDVEITLV

Target ORF information:

RefSeq Version	XM_006507517.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507517.3

ORF Insert Sequence:

ATGTATCCTG TTTGGAAATC TTTTCTCGAG GGAACAATGC AGGTAGCCCA GTCTCGGATC 
AATATATGTG AAAACTATAA AAACTTCATT TCTGAACCTG CAAGAGCAGT AAGAAGCTTA 
AAAGAACAGC AACTAAAACG GTGTGTGGAT CAGTTGACAA AGATCCAAAC AGAATTACAG 
GAAACTGTGA AAGATTTAGT GAAAGGCAAA AAGAAGTACT TTGAGACTGA ACAGATGGCT 
CATGCAGTAA GAGAGAAAGC TGACATCGAA GCAAAGTCTA AACTTAGTCT TTTTCAATCA 
AGAATCAGTT TACAGAAAGC AAGTGTGAAG TTAAAAGCCC GGCGATCTGA ATGTAATACC 
AAAGCTACCC ATGCAAGGAA TGATTACCTT CTGACGCTAG CAGCAGCTAA CGCACATCAG 
GACCGCTACT ATCAAACAGA CTTAGTTAAC ATCATGAAGG CTCTTGATGG AAACGTGTAT 
GACCACCTCA AGGATTATTT AATAGCCTTC AGCCGGACCG AGCTAGAAAC TTGCCAAGCT 
ATACAGAATA CATTCCAGTT TTTACTAGAA AACTCCAGCA AGGTGGTACG GGACTATAAC 
CTTCAGCTGT TTCTACAAGA GAATGCTGTA TTTCACAAGC CTCAGCCCTT CCAGTTCCAG 
CCTTGTGACA GTGATACTAG TTTAAAAGCC GCCCAACTGG TGGATATTGA GCTCTCCCCT 
GTCAGTGCTC TGAGAATGAC GATAGCTGAG AGTCGACAAT TAGAATCAGA AACTGGTACC 
ACAGAGGAAC ACAGTCTAAA TAAGGAGGCT CGGAAATGGG CCACACGTGT GGCACGTGAG 
CACAAAAATA TTGTTCATCA GCAGCGGGTT CTAAATGAAC TAGAATGTCA TGGAGTTGCT 
CTATCAGAAC AGAGCCGAGC AGAGCTGGAA CAGAAAATAG ATGAAGCAAG AGAAAGTATC 
CGTAAAGCAG AGATCATTAA ATTGAAAGCT GAAGCCCGGC TAGACCTACT GAAGCAGATT 
GGGGTTTCTG TAGATACGTG GTTAAAGAGT GCCATGAACC AAGTGATGGA GGAACTAGAA 
AATGAGCGGT GGGCCCGTCC TCCAGCCGTG ACCAGCAATG GCACTCTACA CTCGCTTAAT 
GCAGATGCTG AAAGAGAAGA AGGAGAAGAG TTTGAAGACA ACATGGATGT TTTTGATGAC 
AGCAGCTCCA GCCCTTCTGG CACCTTGAGA AATTATCCAC TCACCTGCAA AGTTGTTTAT 
TCCTATAAGG CTTCTCAACC AGATGAGTTG ACCATTGAGG AACATGAGGT GTTAGAAGTG 
ATTGAAGATG GAGATATGGA AGACTGGGTA AAGGCTCGAA ATAAAGTTGG CCAAGTGGGT 
TATGTGCCAG AAAAGTACCT ACAATTTCCC ACCTCGAACA GCCTACTGAG CATGCTACAG 
TCTTTGGCTG CTTTGGACAG TCGGTCACAC ACATCCAGCA ATTCCACAGA AGCAGAACTC 
GTTTCAGGCA GCCTGAATGG AGATGCCAGT GTCTGTTTTG TGAAAGCACT TTATGATTAT 
GAGGGCCAGA CAGATGATGA GCTGTCCTTT CCCGAGGGAG CAATTATTCG CATCTTGAAC 
AAAGAAAACC AAGATGATGA TGGCTTCTGG GAAGGGGAGT TCAGTGGTCG AATCGGTGTT 
TTCCCATCGG TGCTAGTGGA AGAGCTCTCA GCCTCCGAGA ATGGCGACAC TCCATGGACA 
AGAGAGATCC AGATCTCTCC ATCCCCCAAG CCTCACACAT CCCTGCCTCC ACTGCCTCTG 
TATGACCAGC CACCCAGCAG CCCGTATCCC AGTCCAGATA AGAGGAGCTC CCAGTTCTTC 
CCCCGGTCTC CTTCAGCCAA TGAAAACAGC CTTCATGCTG AATCACCAGG ATTCTCACAG 
GCCTCAAGAC AGACTCCTGA CACCTCATAT GGCAAGCTGC GGCCTGTTCG GGCGGCGCCG 
CCACCACCCA CACAGAACCA CCGGCGGACA ACTGAGAAGA TGGAGGACGT GGAGATCACA 
CTGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fchsd2 ( XM_006507518.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507581.1 Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu39403
Clone ID Related Accession (Same CDS sequence)	XM_006507518.2 , XM_006507518.3
Accession Version	XM_006507518.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	F-BAR and double SH3 domains protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006507518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG CAGTAAGAGA GAAAGCTGAC ATCGAAGCAA AGTCTAAACT TAGTCTTTTT 
CAATCAAGAA TCAGTTTACA GAAAGCAAGT GTGAAGTTAA AAGCCCGGCG ATCTGAATGT 
AATACCAAAG CTACCCATGC AAGGAATGAT TACCTTCTGA CGCTAGCAGC AGCTAACGCA 
CATCAGGACC GCTACTATCA AACAGACTTA GTTAACATCA TGAAGGCTCT TGATGGAAAC 
GTGTATGACC ACCTCAAGGA TTATTTAATA GCCTTCAGCC GGACCGAGCT AGAAACTTGC 
CAAGCTATAC AGAATACATT CCAGTTTTTA CTAGAAAACT CCAGCAAGGT GGTACGGGAC 
TATAACCTTC AGCTGTTTCT ACAAGAGAAT GCTGTATTTC ACAAGCCTCA GCCCTTCCAG 
TTCCAGCCTT GTGACAGTGA TACTAGTTTA AAAGCCGCCC AACTGGTGGA TATTGAGCTC 
TCCCCTGTCA GTGCTCTGAG AATGACGATA GCTGAGAGTC GACAATTAGA ATCAGAAACT 
GGTACCACAG AGGAACACAG TCTAAATAAG GAGGCTCGGA AATGGGCCAC ACGTGTGGCA 
CGTGAGCACA AAAATATTGT TCATCAGCAG CGGGTTCTAA ATGAACTAGA ATGTCATGGA 
GTTGCTCTAT CAGAACAGAG CCGAGCAGAG CTGGAACAGA AAATAGATGA AGCAAGAGAA 
AGTATCCGTA AAGCAGAGAT CATTAAATTG AAAGCTGAAG CCCGGCTAGA CCTACTGAAG 
CAGATTGGGG TTTCTGTAGA TACGTGGTTA AAGAGTGCCA TGAACCAAGT GATGGAGGAA 
CTAGAAAATG AGCGGTGGGC CCGTCCTCCA GCCGTGACCA GCAATGGCAC TCTACACTCG 
CTTAATGCAG ATGCTGAAAG AGAAGAAGGA GAAGAGTTTG AAGACAACAT GGATGTTTTT 
GATGACAGCA GCTCCAGCCC TTCTGGCACC TTGAGAAATT ATCCACTCAC CTGCAAAGTT 
GTTTATTCCT ATAAGGCTTC TCAACCAGAT GAGTTGACCA TTGAGGAACA TGAGGTGTTA 
GAAGTGATTG AAGATGGAGA TATGGAAGAC TGGGTAAAGG CTCGAAATAA AGTTGGCCAA 
GTGGGTTATG TGCCAGAAAA GTACCTACAA TTTCCCACCT CGAACAGCCT ACTGAGCATG 
CTACAGTCTT TGGCTGCTTT GGACAGTCGG TCACACACAT CCAGCAATTC CACAGAAGCA 
GAACTCGTTT CAGGCAGCCT GAATGGAGAT GCCAGTGTCT GTTTTGTGAA AGCACTTTAT 
GATTATGAGG GCCAGACAGA TGATGAGCTG TCCTTTCCCG AGGGAGCAAT TATTCGCATC 
TTGAACAAAG AAAACCAAGA TGATGATGGC TTCTGGGAAG GGGAGTTCAG TGGTCGAATC 
GGTGTTTTCC CATCGGTGCT AGTGGAAGAG CTCTCAGCCT CCGAGAATGG CGACACTCCA 
TGGACAAGAG AGATCCAGAT CTCTCCATCC CCCAAGCCTC ACACATCCCT GCCTCCACTG 
CCTCTGTATG ACCAGCCACC CAGCAGCCCG TATCCCAGTC CAGATAAGAG GAGCTCCCAG 
TTCTTCCCCC GGTCTCCTTC AGCCAATGAA AACAGCCTTC ATGCTGAATC ACCAGGATTC 
TCACAGGCCT CAAGACAGAC TCCTGACACC TCATATGGCA AGCTGCGGCC TGTTCGGGCG 
GCGCCGCCAC CACCCACACA GAACCACCGG CGGACAACTG AGAAGATGGA GGACGTGGAG 
ATCACACTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507581.1
CDS	644..2458
Translation	MAHAVREKADIEAKSKLSLFQSRISLQKASVKLKARRSECNTKATHARNDYLLTLAAANAHQDRYYQTDLVNIMKALDGNVYDHLKDYLIAFSRTELETCQAIQNTFQFLLENSSKVVRDYNLQLFLQENAVFHKPQPFQFQPCDSDTSLKAAQLVDIELSPVSALRMTIAESRQLESETGTTEEHSLNKEARKWATRVAREHKNIVHQQRVLNELECHGVALSEQSRAELEQKIDEARESIRKAEIIKLKAEARLDLLKQIGVSVDTWLKSAMNQVMEELENERWARPPAVTSNGTLHSLNADAEREEGEEFEDNMDVFDDSSSSPSGTLRNYPLTCKVVYSYKASQPDELTIEEHEVLEVIEDGDMEDWVKARNKVGQVGYVPEKYLQFPTSNSLLSMLQSLAALDSRSHTSSNSTEAELVSGSLNGDASVCFVKALYDYEGQTDDELSFPEGAIIRILNKENQDDDGFWEGEFSGRIGVFPSVLVEELSASENGDTPWTREIQISPSPKPHTSLPPLPLYDQPPSSPYPSPDKRSSQFFPRSPSANENSLHAESPGFSQASRQTPDTSYGKLRPVRAAPPPPTQNHRRTTEKMEDVEITLV

Target ORF information:

RefSeq Version	XM_006507518.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507518.2

ORF Insert Sequence:

ATGGCTCATG CAGTAAGAGA GAAAGCTGAC ATCGAAGCAA AGTCTAAACT TAGTCTTTTT 
CAATCAAGAA TCAGTTTACA GAAAGCAAGT GTGAAGTTAA AAGCCCGGCG ATCTGAATGT 
AATACCAAAG CTACCCATGC AAGGAATGAT TACCTTCTGA CGCTAGCAGC AGCTAACGCA 
CATCAGGACC GCTACTATCA AACAGACTTA GTTAACATCA TGAAGGCTCT TGATGGAAAC 
GTGTATGACC ACCTCAAGGA TTATTTAATA GCCTTCAGCC GGACCGAGCT AGAAACTTGC 
CAAGCTATAC AGAATACATT CCAGTTTTTA CTAGAAAACT CCAGCAAGGT GGTACGGGAC 
TATAACCTTC AGCTGTTTCT ACAAGAGAAT GCTGTATTTC ACAAGCCTCA GCCCTTCCAG 
TTCCAGCCTT GTGACAGTGA TACTAGTTTA AAAGCCGCCC AACTGGTGGA TATTGAGCTC 
TCCCCTGTCA GTGCTCTGAG AATGACGATA GCTGAGAGTC GACAATTAGA ATCAGAAACT 
GGTACCACAG AGGAACACAG TCTAAATAAG GAGGCTCGGA AATGGGCCAC ACGTGTGGCA 
CGTGAGCACA AAAATATTGT TCATCAGCAG CGGGTTCTAA ATGAACTAGA ATGTCATGGA 
GTTGCTCTAT CAGAACAGAG CCGAGCAGAG CTGGAACAGA AAATAGATGA AGCAAGAGAA 
AGTATCCGTA AAGCAGAGAT CATTAAATTG AAAGCTGAAG CCCGGCTAGA CCTACTGAAG 
CAGATTGGGG TTTCTGTAGA TACGTGGTTA AAGAGTGCCA TGAACCAAGT GATGGAGGAA 
CTAGAAAATG AGCGGTGGGC CCGTCCTCCA GCCGTGACCA GCAATGGCAC TCTACACTCG 
CTTAATGCAG ATGCTGAAAG AGAAGAAGGA GAAGAGTTTG AAGACAACAT GGATGTTTTT 
GATGACAGCA GCTCCAGCCC TTCTGGCACC TTGAGAAATT ATCCACTCAC CTGCAAAGTT 
GTTTATTCCT ATAAGGCTTC TCAACCAGAT GAGTTGACCA TTGAGGAACA TGAGGTGTTA 
GAAGTGATTG AAGATGGAGA TATGGAAGAC TGGGTAAAGG CTCGAAATAA AGTTGGCCAA 
GTGGGTTATG TGCCAGAAAA GTACCTACAA TTTCCCACCT CGAACAGCCT ACTGAGCATG 
CTACAGTCTT TGGCTGCTTT GGACAGTCGG TCACACACAT CCAGCAATTC CACAGAAGCA 
GAACTCGTTT CAGGCAGCCT GAATGGAGAT GCCAGTGTCT GTTTTGTGAA AGCACTTTAT 
GATTATGAGG GCCAGACAGA TGATGAGCTG TCCTTTCCCG AGGGAGCAAT TATTCGCATC 
TTGAACAAAG AAAACCAAGA TGATGATGGC TTCTGGGAAG GGGAGTTCAG TGGTCGAATC 
GGTGTTTTCC CATCGGTGCT AGTGGAAGAG CTCTCAGCCT CCGAGAATGG CGACACTCCA 
TGGACAAGAG AGATCCAGAT CTCTCCATCC CCCAAGCCTC ACACATCCCT GCCTCCACTG 
CCTCTGTATG ACCAGCCACC CAGCAGCCCG TATCCCAGTC CAGATAAGAG GAGCTCCCAG 
TTCTTCCCCC GGTCTCCTTC AGCCAATGAA AACAGCCTTC ATGCTGAATC ACCAGGATTC 
TCACAGGCCT CAAGACAGAC TCCTGACACC TCATATGGCA AGCTGCGGCC TGTTCGGGCG 
GCGCCGCCAC CACCCACACA GAACCACCGG CGGACAACTG AGAAGATGGA GGACGTGGAG 
ATCACACTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fchsd2 ( NM_001146010.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139482.1 Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07138
Clone ID Related Accession (Same CDS sequence)	NM_001146010.1
Accession Version	NM_001146010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	F-BAR and double SH3 domains protein 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK141949.1, AK140322.1 and BC060647.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the mid coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK141949.1, SRR1660815.43554.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCGC CGCCGAGGAA GGTGAAAGTT ACACAAGAGC TGAGAAACAT TCAAGGTGAG 
CAGATGACAA AACTTCAAGC CAAACATCAG GCAGAATGTG ACTTACTTGA AGACATGAGG 
ACTTTCAGTC AGAAGAAGGC TGCTATTGAA AGAGAGTATG CACAGGGTAT CCAGAAGTTG 
GCTAGTCAAT ACCTGAAGAG AGATTGGCCT GGAATAAAAA CTGATGATCG GAATGATTAC 
AGGAGTATGT ATCCTGTTTG GAAATCTTTT CTCGAGGGAA CAATGCAGGT AGCCCAGTCT 
CGGATCAATA TATGTGAAAA CTATAAAAAC TTCATTTCTG AACCTGCAAG AGCAGTAAGA 
AGCTTAAAAG AACAGCAACT AAAACGGTGT GTGGATCAGT TGACAAAGAT CCAAACAGAA 
TTACAGGAAA CTGTGAAAGA TTTAGTGAAA GGCAAAAAGA AGTACTTTGA GACTGAACAG 
ATGGCTCATG CAGTAAGAGA GAAAGCTGAC ATCGAAGCAA AGTCTAAACT TAGTCTTTTT 
CAATCAAGAA TCAGTTTACA GAAAGCAAGT GTGAAGTTAA AAGCCCGGCG ATCTGAATGT 
AATACCAAAG CTACCCATGC AAGGAATGAT TACCTTCTGA CGCTAGCAGC AGCTAACGCA 
CATCAGGACC GCTACTATCA AACAGACTTA GTTAACATCA TGAAGGCTCT TGATGGAAAC 
GTGTATGACC ACCTCAAGGA TTATTTAATA GCCTTCAGCC GGACCGAGCT AGAAACTTGC 
CAAGCTATAC AGAATACATT CCAGTTTTTA CTAGAAAACT CCAGCAAGGT GGTACGGGAC 
TATAACCTTC AGCTGTTTCT ACAAGAGAAT GCTGTATTTC ACAAGCCTCA GCCCTTCCAG 
TTCCAGCCTT GTGACAGTGA TACTAGTCGA CAATTAGAAT CAGAAACTGG TACCACAGAG 
GAACACAGTC TAAATAAGGA GGCTCGGAAA TGGGCCACAC GTGTGGCACG TGAGCACAAA 
AATATTGTTC ATCAGCAGCG GGTTCTAAAT GAACTAGAAT GTCATGGAGT TGCTCTATCA 
GAACAGAGCC GAGCAGAGCT GGAACAGAAA ATAGATGAAG CAAGAGAAAG TATCCGTAAA 
GCAGAGATCA TTAAATTGAA AGCTGAAGCC CGGCTAGACC TACTGAAGCA GATTGGGGTT 
TCTGTAGATA CGTGGTTAAA GAGTGCCATG AACCAAGTGA TGGAGGAACT AGAAAATGAG 
CGGTGGGCCC GTCCTCCAGC CGTGACCAGC AATGGCACTC TACACTCGCT TAATGCAGAT 
GCTGAAAGAG AAGAAGGAGA AGAGTTTGAA GACAACATGG ATGTTTTTGA TGACAGCAGC 
TCCAGCCCTT CTGGCACCTT GAGAAATTAT CCACTCACCT GCAAAGTTGT TTATTCCTAT 
AAGGCTTCTC AACCAGATGA GTTGACCATT GAGGAACATG AGGTGTTAGA AGTGATTGAA 
GATGGAGATA TGGAAGACTG GGTAAAGGCT CGAAATAAAG TTGGCCAAGT GGGTTATGTG 
CCAGAAAAGT ACCTACAATT TCCCACCTCG AACAGCCTAC TGAGCATGCT ACAGTCTTTG 
GCTGCTTTGG ACAGTCGGTC ACACACATCC AGCAATTCCA CAGAAGCAGA ACTCGTTTCA 
GGCAGCCTGA ATGGAGATGC CAGTGTCTGT TTTGTGAAAG CACTTTATGA TTATGAGGGC 
CAGACAGATG ATGAGCTGTC CTTTCCCGAG GGAGCAATTA TTCGCATCTT GAACAAAGAA 
AACCAAGATG ATGATGGCTT CTGGGAAGGG GAGTTCAGTG GTCGAATCGG TGTTTTCCCA 
TCGGTGCTAG TGGAAGAGCT CTCAGCCTCC GAGAATGGCG ACACTCCATG GACAAGAGAG 
ATCCAGATCT CTCCATCCCC CAAGCCTCAC ACATCCCTGC CTCCACTGCC TCTGTATGAC 
CAGCCACCCA GCAGCCCGTA TCCCAGTCCA GATAAGAGGA GCTCCCAGTT CTTCCCCCGG 
TCTCCTTCAG CCAATGAAAA CAGCCTTCAT GCTGAATCAC CAGGATTCTC ACAGGCCTCA 
AGACAGACTC CTGACACCTC ATATGGCAAG CTGCGGCCTG TTCGGGCGGC GCCGCCACCA 
CCCACACAGA ACCACCGGCG GACAACTGAG AAGATGGAGG ACGTGGAGAT CACACTGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139482.1
CDS	245..2467
Translation	MQPPPRKVKVTQELRNIQGEQMTKLQAKHQAECDLLEDMRTFSQKKAAIEREYAQGIQKLASQYLKRDWPGIKTDDRNDYRSMYPVWKSFLEGTMQVAQSRINICENYKNFISEPARAVRSLKEQQLKRCVDQLTKIQTELQETVKDLVKGKKKYFETEQMAHAVREKADIEAKSKLSLFQSRISLQKASVKLKARRSECNTKATHARNDYLLTLAAANAHQDRYYQTDLVNIMKALDGNVYDHLKDYLIAFSRTELETCQAIQNTFQFLLENSSKVVRDYNLQLFLQENAVFHKPQPFQFQPCDSDTSRQLESETGTTEEHSLNKEARKWATRVAREHKNIVHQQRVLNELECHGVALSEQSRAELEQKIDEARESIRKAEIIKLKAEARLDLLKQIGVSVDTWLKSAMNQVMEELENERWARPPAVTSNGTLHSLNADAEREEGEEFEDNMDVFDDSSSSPSGTLRNYPLTCKVVYSYKASQPDELTIEEHEVLEVIEDGDMEDWVKARNKVGQVGYVPEKYLQFPTSNSLLSMLQSLAALDSRSHTSSNSTEAELVSGSLNGDASVCFVKALYDYEGQTDDELSFPEGAIIRILNKENQDDDGFWEGEFSGRIGVFPSVLVEELSASENGDTPWTREIQISPSPKPHTSLPPLPLYDQPPSSPYPSPDKRSSQFFPRSPSANENSLHAESPGFSQASRQTPDTSYGKLRPVRAAPPPPTQNHRRTTEKMEDVEITLV

Target ORF information:

RefSeq Version	NM_001146010.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146010.1

ORF Insert Sequence:

ATGCAGCCGC CGCCGAGGAA GGTGAAAGTT ACACAAGAGC TGAGAAACAT TCAAGGTGAG 
CAGATGACAA AACTTCAAGC CAAACATCAG GCAGAATGTG ACTTACTTGA AGACATGAGG 
ACTTTCAGTC AGAAGAAGGC TGCTATTGAA AGAGAGTATG CACAGGGTAT CCAGAAGTTG 
GCTAGTCAAT ACCTGAAGAG AGATTGGCCT GGAATAAAAA CTGATGATCG GAATGATTAC 
AGGAGTATGT ATCCTGTTTG GAAATCTTTT CTCGAGGGAA CAATGCAGGT AGCCCAGTCT 
CGGATCAATA TATGTGAAAA CTATAAAAAC TTCATTTCTG AACCTGCAAG AGCAGTAAGA 
AGCTTAAAAG AACAGCAACT AAAACGGTGT GTGGATCAGT TGACAAAGAT CCAAACAGAA 
TTACAGGAAA CTGTGAAAGA TTTAGTGAAA GGCAAAAAGA AGTACTTTGA GACTGAACAG 
ATGGCTCATG CAGTAAGAGA GAAAGCTGAC ATCGAAGCAA AGTCTAAACT TAGTCTTTTT 
CAATCAAGAA TCAGTTTACA GAAAGCAAGT GTGAAGTTAA AAGCCCGGCG ATCTGAATGT 
AATACCAAAG CTACCCATGC AAGGAATGAT TACCTTCTGA CGCTAGCAGC AGCTAACGCA 
CATCAGGACC GCTACTATCA AACAGACTTA GTTAACATCA TGAAGGCTCT TGATGGAAAC 
GTGTATGACC ACCTCAAGGA TTATTTAATA GCCTTCAGCC GGACCGAGCT AGAAACTTGC 
CAAGCTATAC AGAATACATT CCAGTTTTTA CTAGAAAACT CCAGCAAGGT GGTACGGGAC 
TATAACCTTC AGCTGTTTCT ACAAGAGAAT GCTGTATTTC ACAAGCCTCA GCCCTTCCAG 
TTCCAGCCTT GTGACAGTGA TACTAGTCGA CAATTAGAAT CAGAAACTGG TACCACAGAG 
GAACACAGTC TAAATAAGGA GGCTCGGAAA TGGGCCACAC GTGTGGCACG TGAGCACAAA 
AATATTGTTC ATCAGCAGCG GGTTCTAAAT GAACTAGAAT GTCATGGAGT TGCTCTATCA 
GAACAGAGCC GAGCAGAGCT GGAACAGAAA ATAGATGAAG CAAGAGAAAG TATCCGTAAA 
GCAGAGATCA TTAAATTGAA AGCTGAAGCC CGGCTAGACC TACTGAAGCA GATTGGGGTT 
TCTGTAGATA CGTGGTTAAA GAGTGCCATG AACCAAGTGA TGGAGGAACT AGAAAATGAG 
CGGTGGGCCC GTCCTCCAGC CGTGACCAGC AATGGCACTC TACACTCGCT TAATGCAGAT 
GCTGAAAGAG AAGAAGGAGA AGAGTTTGAA GACAACATGG ATGTTTTTGA TGACAGCAGC 
TCCAGCCCTT CTGGCACCTT GAGAAATTAT CCACTCACCT GCAAAGTTGT TTATTCCTAT 
AAGGCTTCTC AACCAGATGA GTTGACCATT GAGGAACATG AGGTGTTAGA AGTGATTGAA 
GATGGAGATA TGGAAGACTG GGTAAAGGCT CGAAATAAAG TTGGCCAAGT GGGTTATGTG 
CCAGAAAAGT ACCTACAATT TCCCACCTCG AACAGCCTAC TGAGCATGCT ACAGTCTTTG 
GCTGCTTTGG ACAGTCGGTC ACACACATCC AGCAATTCCA CAGAAGCAGA ACTCGTTTCA 
GGCAGCCTGA ATGGAGATGC CAGTGTCTGT TTTGTGAAAG CACTTTATGA TTATGAGGGC 
CAGACAGATG ATGAGCTGTC CTTTCCCGAG GGAGCAATTA TTCGCATCTT GAACAAAGAA 
AACCAAGATG ATGATGGCTT CTGGGAAGGG GAGTTCAGTG GTCGAATCGG TGTTTTCCCA 
TCGGTGCTAG TGGAAGAGCT CTCAGCCTCC GAGAATGGCG ACACTCCATG GACAAGAGAG 
ATCCAGATCT CTCCATCCCC CAAGCCTCAC ACATCCCTGC CTCCACTGCC TCTGTATGAC 
CAGCCACCCA GCAGCCCGTA TCCCAGTCCA GATAAGAGGA GCTCCCAGTT CTTCCCCCGG 
TCTCCTTCAG CCAATGAAAA CAGCCTTCAT GCTGAATCAC CAGGATTCTC ACAGGCCTCA 
AGACAGACTC CTGACACCTC ATATGGCAAG CTGCGGCCTG TTCGGGCGGC GCCGCCACCA 
CCCACACAGA ACCACCGGCG GACAACTGAG AAGATGGAGG ACGTGGAGAT CACACTGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fchsd2 ( NM_199012.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_950177.2 Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant 1, mRNA.

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CloneID	OMu09266
Clone ID Related Accession (Same CDS sequence)	NM_199012.2
Accession Version	NM_199012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	F-BAR and double SH3 domains protein 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK141949.1 and BC060647.1. On Mar 24, 2009 this sequence version replaced NM_199012.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC060647.1, AK122368.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCGC CGCCGAGGAA GGTGAAAGTT ACACAAGAGC TGAGAAACAT TCAAGGTGAG 
CAGATGACAA AACTTCAAGC CAAACATCAG GCAGAATGTG ACTTACTTGA AGACATGAGG 
ACTTTCAGTC AGAAGAAGGC TGCTATTGAA AGAGAGTATG CACAGGGTAT CCAGAAGTTG 
GCTAGTCAAT ACCTGAAGAG AGATTGGCCT GGAATAAAAA CTGATGATCG GAATGATTAC 
AGGAGTATGT ATCCTGTTTG GAAATCTTTT CTCGAGGGAA CAATGCAGGT AGCCCAGTCT 
CGGATCAATA TATGTGAAAA CTATAAAAAC TTCATTTCTG AACCTGCAAG AGCAGTAAGA 
AGCTTAAAAG AACAGCAACT AAAACGGTGT GTGGATCAGT TGACAAAGAT CCAAACAGAA 
TTACAGGAAA CTGTGAAAGA TTTAGTGAAA GGCAAAAAGA AGTACTTTGA GACTGAACAG 
ATGGCTCATG CAGTAAGAGA GAAAGCTGAC ATCGAAGCAA AGTCTAAACT TAGTCTTTTT 
CAATCAAGAA TCAGTTTACA GAAAGCAAGT GTGAAGTTAA AAGCCCGGCG ATCTGAATGT 
AATACCAAAG CTACCCATGC AAGGAATGAT TACCTTCTGA CGCTAGCAGC AGCTAACGCA 
CATCAGGACC GCTACTATCA AACAGACTTA GTTAACATCA TGAAGGCTCT TGATGGAAAC 
GTGTATGACC ACCTCAAGGA TTATTTAATA GCCTTCAGCC GGACCGAGCT AGAAACTTGC 
CAAGCTATAC AGAATACATT CCAGTTTTTA CTAGAAAACT CCAGCAAGGT GGTACGGGAC 
TATAACCTTC AGCTGTTTCT ACAAGAGAAT GCTGTATTTC ACAAGCCTCA GCCCTTCCAG 
TTCCAGCCTT GTGACAGTGA TACTAGTTTA AAAGCCGCCC AACTGGTGGA TATTGAGCTC 
TCCCCTGTCA GTGCTCTGAG AATGACGATA GCTGAGAGTC GACAATTAGA ATCAGAAACT 
GGTACCACAG AGGAACACAG TCTAAATAAG GAGGCTCGGA AATGGGCCAC ACGTGTGGCA 
CGTGAGCACA AAAATATTGT TCATCAGCAG CGGGTTCTAA ATGAACTAGA ATGTCATGGA 
GTTGCTCTAT CAGAACAGAG CCGAGCAGAG CTGGAACAGA AAATAGATGA AGCAAGAGAA 
AGTATCCGTA AAGCAGAGAT CATTAAATTG AAAGCTGAAG CCCGGCTAGA CCTACTGAAG 
CAGATTGGGG TTTCTGTAGA TACGTGGTTA AAGAGTGCCA TGAACCAAGT GATGGAGGAA 
CTAGAAAATG AGCGGTGGGC CCGTCCTCCA GCCGTGACCA GCAATGGCAC TCTACACTCG 
CTTAATGCAG ATGCTGAAAG AGAAGAAGGA GAAGAGTTTG AAGACAACAT GGATGTTTTT 
GATGACAGCA GCTCCAGCCC TTCTGGCACC TTGAGAAATT ATCCACTCAC CTGCAAAGTT 
GTTTATTCCT ATAAGGCTTC TCAACCAGAT GAGTTGACCA TTGAGGAACA TGAGGTGTTA 
GAAGTGATTG AAGATGGAGA TATGGAAGAC TGGGTAAAGG CTCGAAATAA AGTTGGCCAA 
GTGGGTTATG TGCCAGAAAA GTACCTACAA TTTCCCACCT CGAACAGCCT ACTGAGCATG 
CTACAGTCTT TGGCTGCTTT GGACAGTCGG TCACACACAT CCAGCAATTC CACAGAAGCA 
GAACTCGTTT CAGGCAGCCT GAATGGAGAT GCCAGTGTCT GTTTTGTGAA AGCACTTTAT 
GATTATGAGG GCCAGACAGA TGATGAGCTG TCCTTTCCCG AGGGAGCAAT TATTCGCATC 
TTGAACAAAG AAAACCAAGA TGATGATGGC TTCTGGGAAG GGGAGTTCAG TGGTCGAATC 
GGTGTTTTCC CATCGGTGCT AGTGGAAGAG CTCTCAGCCT CCGAGAATGG CGACACTCCA 
TGGACAAGAG AGATCCAGAT CTCTCCATCC CCCAAGCCTC ACACATCCCT GCCTCCACTG 
CCTCTGTATG ACCAGCCACC CAGCAGCCCG TATCCCAGTC CAGATAAGAG GAGCTCCCAG 
TTCTTCCCCC GGTCTCCTTC AGCCAATGAA AACAGCCTTC ATGCTGAATC ACCAGGATTC 
TCACAGGCCT CAAGACAGAC TCCTGACACC TCATATGGCA AGCTGCGGCC TGTTCGGGCG 
GCGCCGCCAC CACCCACACA GAACCACCGG CGGACAACTG AGAAGATGGA GGACGTGGAG 
ATCACACTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_950177.2
CDS	245..2539
Translation	MQPPPRKVKVTQELRNIQGEQMTKLQAKHQAECDLLEDMRTFSQKKAAIEREYAQGIQKLASQYLKRDWPGIKTDDRNDYRSMYPVWKSFLEGTMQVAQSRINICENYKNFISEPARAVRSLKEQQLKRCVDQLTKIQTELQETVKDLVKGKKKYFETEQMAHAVREKADIEAKSKLSLFQSRISLQKASVKLKARRSECNTKATHARNDYLLTLAAANAHQDRYYQTDLVNIMKALDGNVYDHLKDYLIAFSRTELETCQAIQNTFQFLLENSSKVVRDYNLQLFLQENAVFHKPQPFQFQPCDSDTSLKAAQLVDIELSPVSALRMTIAESRQLESETGTTEEHSLNKEARKWATRVAREHKNIVHQQRVLNELECHGVALSEQSRAELEQKIDEARESIRKAEIIKLKAEARLDLLKQIGVSVDTWLKSAMNQVMEELENERWARPPAVTSNGTLHSLNADAEREEGEEFEDNMDVFDDSSSSPSGTLRNYPLTCKVVYSYKASQPDELTIEEHEVLEVIEDGDMEDWVKARNKVGQVGYVPEKYLQFPTSNSLLSMLQSLAALDSRSHTSSNSTEAELVSGSLNGDASVCFVKALYDYEGQTDDELSFPEGAIIRILNKENQDDDGFWEGEFSGRIGVFPSVLVEELSASENGDTPWTREIQISPSPKPHTSLPPLPLYDQPPSSPYPSPDKRSSQFFPRSPSANENSLHAESPGFSQASRQTPDTSYGKLRPVRAAPPPPTQNHRRTTEKMEDVEITLV

Target ORF information:

RefSeq Version	NM_199012.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199012.2

ORF Insert Sequence:

ATGCAGCCGC CGCCGAGGAA GGTGAAAGTT ACACAAGAGC TGAGAAACAT TCAAGGTGAG 
CAGATGACAA AACTTCAAGC CAAACATCAG GCAGAATGTG ACTTACTTGA AGACATGAGG 
ACTTTCAGTC AGAAGAAGGC TGCTATTGAA AGAGAGTATG CACAGGGTAT CCAGAAGTTG 
GCTAGTCAAT ACCTGAAGAG AGATTGGCCT GGAATAAAAA CTGATGATCG GAATGATTAC 
AGGAGTATGT ATCCTGTTTG GAAATCTTTT CTCGAGGGAA CAATGCAGGT AGCCCAGTCT 
CGGATCAATA TATGTGAAAA CTATAAAAAC TTCATTTCTG AACCTGCAAG AGCAGTAAGA 
AGCTTAAAAG AACAGCAACT AAAACGGTGT GTGGATCAGT TGACAAAGAT CCAAACAGAA 
TTACAGGAAA CTGTGAAAGA TTTAGTGAAA GGCAAAAAGA AGTACTTTGA GACTGAACAG 
ATGGCTCATG CAGTAAGAGA GAAAGCTGAC ATCGAAGCAA AGTCTAAACT TAGTCTTTTT 
CAATCAAGAA TCAGTTTACA GAAAGCAAGT GTGAAGTTAA AAGCCCGGCG ATCTGAATGT 
AATACCAAAG CTACCCATGC AAGGAATGAT TACCTTCTGA CGCTAGCAGC AGCTAACGCA 
CATCAGGACC GCTACTATCA AACAGACTTA GTTAACATCA TGAAGGCTCT TGATGGAAAC 
GTGTATGACC ACCTCAAGGA TTATTTAATA GCCTTCAGCC GGACCGAGCT AGAAACTTGC 
CAAGCTATAC AGAATACATT CCAGTTTTTA CTAGAAAACT CCAGCAAGGT GGTACGGGAC 
TATAACCTTC AGCTGTTTCT ACAAGAGAAT GCTGTATTTC ACAAGCCTCA GCCCTTCCAG 
TTCCAGCCTT GTGACAGTGA TACTAGTTTA AAAGCCGCCC AACTGGTGGA TATTGAGCTC 
TCCCCTGTCA GTGCTCTGAG AATGACGATA GCTGAGAGTC GACAATTAGA ATCAGAAACT 
GGTACCACAG AGGAACACAG TCTAAATAAG GAGGCTCGGA AATGGGCCAC ACGTGTGGCA 
CGTGAGCACA AAAATATTGT TCATCAGCAG CGGGTTCTAA ATGAACTAGA ATGTCATGGA 
GTTGCTCTAT CAGAACAGAG CCGAGCAGAG CTGGAACAGA AAATAGATGA AGCAAGAGAA 
AGTATCCGTA AAGCAGAGAT CATTAAATTG AAAGCTGAAG CCCGGCTAGA CCTACTGAAG 
CAGATTGGGG TTTCTGTAGA TACGTGGTTA AAGAGTGCCA TGAACCAAGT GATGGAGGAA 
CTAGAAAATG AGCGGTGGGC CCGTCCTCCA GCCGTGACCA GCAATGGCAC TCTACACTCG 
CTTAATGCAG ATGCTGAAAG AGAAGAAGGA GAAGAGTTTG AAGACAACAT GGATGTTTTT 
GATGACAGCA GCTCCAGCCC TTCTGGCACC TTGAGAAATT ATCCACTCAC CTGCAAAGTT 
GTTTATTCCT ATAAGGCTTC TCAACCAGAT GAGTTGACCA TTGAGGAACA TGAGGTGTTA 
GAAGTGATTG AAGATGGAGA TATGGAAGAC TGGGTAAAGG CTCGAAATAA AGTTGGCCAA 
GTGGGTTATG TGCCAGAAAA GTACCTACAA TTTCCCACCT CGAACAGCCT ACTGAGCATG 
CTACAGTCTT TGGCTGCTTT GGACAGTCGG TCACACACAT CCAGCAATTC CACAGAAGCA 
GAACTCGTTT CAGGCAGCCT GAATGGAGAT GCCAGTGTCT GTTTTGTGAA AGCACTTTAT 
GATTATGAGG GCCAGACAGA TGATGAGCTG TCCTTTCCCG AGGGAGCAAT TATTCGCATC 
TTGAACAAAG AAAACCAAGA TGATGATGGC TTCTGGGAAG GGGAGTTCAG TGGTCGAATC 
GGTGTTTTCC CATCGGTGCT AGTGGAAGAG CTCTCAGCCT CCGAGAATGG CGACACTCCA 
TGGACAAGAG AGATCCAGAT CTCTCCATCC CCCAAGCCTC ACACATCCCT GCCTCCACTG 
CCTCTGTATG ACCAGCCACC CAGCAGCCCG TATCCCAGTC CAGATAAGAG GAGCTCCCAG 
TTCTTCCCCC GGTCTCCTTC AGCCAATGAA AACAGCCTTC ATGCTGAATC ACCAGGATTC 
TCACAGGCCT CAAGACAGAC TCCTGACACC TCATATGGCA AGCTGCGGCC TGTTCGGGCG 
GCGCCGCCAC CACCCACACA GAACCACCGG CGGACAACTG AGAAGATGGA GGACGTGGAG 
ATCACACTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fchsd2 ( XM_006507518.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507581.1 Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant X3, mRNA.

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CloneID	OMu39403
Clone ID Related Accession (Same CDS sequence)	XM_006507518.2 , XM_006507518.3
Accession Version	XM_006507518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	F-BAR and double SH3 domains protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507518.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG CAGTAAGAGA GAAAGCTGAC ATCGAAGCAA AGTCTAAACT TAGTCTTTTT 
CAATCAAGAA TCAGTTTACA GAAAGCAAGT GTGAAGTTAA AAGCCCGGCG ATCTGAATGT 
AATACCAAAG CTACCCATGC AAGGAATGAT TACCTTCTGA CGCTAGCAGC AGCTAACGCA 
CATCAGGACC GCTACTATCA AACAGACTTA GTTAACATCA TGAAGGCTCT TGATGGAAAC 
GTGTATGACC ACCTCAAGGA TTATTTAATA GCCTTCAGCC GGACCGAGCT AGAAACTTGC 
CAAGCTATAC AGAATACATT CCAGTTTTTA CTAGAAAACT CCAGCAAGGT GGTACGGGAC 
TATAACCTTC AGCTGTTTCT ACAAGAGAAT GCTGTATTTC ACAAGCCTCA GCCCTTCCAG 
TTCCAGCCTT GTGACAGTGA TACTAGTTTA AAAGCCGCCC AACTGGTGGA TATTGAGCTC 
TCCCCTGTCA GTGCTCTGAG AATGACGATA GCTGAGAGTC GACAATTAGA ATCAGAAACT 
GGTACCACAG AGGAACACAG TCTAAATAAG GAGGCTCGGA AATGGGCCAC ACGTGTGGCA 
CGTGAGCACA AAAATATTGT TCATCAGCAG CGGGTTCTAA ATGAACTAGA ATGTCATGGA 
GTTGCTCTAT CAGAACAGAG CCGAGCAGAG CTGGAACAGA AAATAGATGA AGCAAGAGAA 
AGTATCCGTA AAGCAGAGAT CATTAAATTG AAAGCTGAAG CCCGGCTAGA CCTACTGAAG 
CAGATTGGGG TTTCTGTAGA TACGTGGTTA AAGAGTGCCA TGAACCAAGT GATGGAGGAA 
CTAGAAAATG AGCGGTGGGC CCGTCCTCCA GCCGTGACCA GCAATGGCAC TCTACACTCG 
CTTAATGCAG ATGCTGAAAG AGAAGAAGGA GAAGAGTTTG AAGACAACAT GGATGTTTTT 
GATGACAGCA GCTCCAGCCC TTCTGGCACC TTGAGAAATT ATCCACTCAC CTGCAAAGTT 
GTTTATTCCT ATAAGGCTTC TCAACCAGAT GAGTTGACCA TTGAGGAACA TGAGGTGTTA 
GAAGTGATTG AAGATGGAGA TATGGAAGAC TGGGTAAAGG CTCGAAATAA AGTTGGCCAA 
GTGGGTTATG TGCCAGAAAA GTACCTACAA TTTCCCACCT CGAACAGCCT ACTGAGCATG 
CTACAGTCTT TGGCTGCTTT GGACAGTCGG TCACACACAT CCAGCAATTC CACAGAAGCA 
GAACTCGTTT CAGGCAGCCT GAATGGAGAT GCCAGTGTCT GTTTTGTGAA AGCACTTTAT 
GATTATGAGG GCCAGACAGA TGATGAGCTG TCCTTTCCCG AGGGAGCAAT TATTCGCATC 
TTGAACAAAG AAAACCAAGA TGATGATGGC TTCTGGGAAG GGGAGTTCAG TGGTCGAATC 
GGTGTTTTCC CATCGGTGCT AGTGGAAGAG CTCTCAGCCT CCGAGAATGG CGACACTCCA 
TGGACAAGAG AGATCCAGAT CTCTCCATCC CCCAAGCCTC ACACATCCCT GCCTCCACTG 
CCTCTGTATG ACCAGCCACC CAGCAGCCCG TATCCCAGTC CAGATAAGAG GAGCTCCCAG 
TTCTTCCCCC GGTCTCCTTC AGCCAATGAA AACAGCCTTC ATGCTGAATC ACCAGGATTC 
TCACAGGCCT CAAGACAGAC TCCTGACACC TCATATGGCA AGCTGCGGCC TGTTCGGGCG 
GCGCCGCCAC CACCCACACA GAACCACCGG CGGACAACTG AGAAGATGGA GGACGTGGAG 
ATCACACTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507581.1
CDS	856..2670
Translation	MAHAVREKADIEAKSKLSLFQSRISLQKASVKLKARRSECNTKATHARNDYLLTLAAANAHQDRYYQTDLVNIMKALDGNVYDHLKDYLIAFSRTELETCQAIQNTFQFLLENSSKVVRDYNLQLFLQENAVFHKPQPFQFQPCDSDTSLKAAQLVDIELSPVSALRMTIAESRQLESETGTTEEHSLNKEARKWATRVAREHKNIVHQQRVLNELECHGVALSEQSRAELEQKIDEARESIRKAEIIKLKAEARLDLLKQIGVSVDTWLKSAMNQVMEELENERWARPPAVTSNGTLHSLNADAEREEGEEFEDNMDVFDDSSSSPSGTLRNYPLTCKVVYSYKASQPDELTIEEHEVLEVIEDGDMEDWVKARNKVGQVGYVPEKYLQFPTSNSLLSMLQSLAALDSRSHTSSNSTEAELVSGSLNGDASVCFVKALYDYEGQTDDELSFPEGAIIRILNKENQDDDGFWEGEFSGRIGVFPSVLVEELSASENGDTPWTREIQISPSPKPHTSLPPLPLYDQPPSSPYPSPDKRSSQFFPRSPSANENSLHAESPGFSQASRQTPDTSYGKLRPVRAAPPPPTQNHRRTTEKMEDVEITLV

Target ORF information:

RefSeq Version	XM_006507518.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507518.3

ORF Insert Sequence:

ATGGCTCATG CAGTAAGAGA GAAAGCTGAC ATCGAAGCAA AGTCTAAACT TAGTCTTTTT 
CAATCAAGAA TCAGTTTACA GAAAGCAAGT GTGAAGTTAA AAGCCCGGCG ATCTGAATGT 
AATACCAAAG CTACCCATGC AAGGAATGAT TACCTTCTGA CGCTAGCAGC AGCTAACGCA 
CATCAGGACC GCTACTATCA AACAGACTTA GTTAACATCA TGAAGGCTCT TGATGGAAAC 
GTGTATGACC ACCTCAAGGA TTATTTAATA GCCTTCAGCC GGACCGAGCT AGAAACTTGC 
CAAGCTATAC AGAATACATT CCAGTTTTTA CTAGAAAACT CCAGCAAGGT GGTACGGGAC 
TATAACCTTC AGCTGTTTCT ACAAGAGAAT GCTGTATTTC ACAAGCCTCA GCCCTTCCAG 
TTCCAGCCTT GTGACAGTGA TACTAGTTTA AAAGCCGCCC AACTGGTGGA TATTGAGCTC 
TCCCCTGTCA GTGCTCTGAG AATGACGATA GCTGAGAGTC GACAATTAGA ATCAGAAACT 
GGTACCACAG AGGAACACAG TCTAAATAAG GAGGCTCGGA AATGGGCCAC ACGTGTGGCA 
CGTGAGCACA AAAATATTGT TCATCAGCAG CGGGTTCTAA ATGAACTAGA ATGTCATGGA 
GTTGCTCTAT CAGAACAGAG CCGAGCAGAG CTGGAACAGA AAATAGATGA AGCAAGAGAA 
AGTATCCGTA AAGCAGAGAT CATTAAATTG AAAGCTGAAG CCCGGCTAGA CCTACTGAAG 
CAGATTGGGG TTTCTGTAGA TACGTGGTTA AAGAGTGCCA TGAACCAAGT GATGGAGGAA 
CTAGAAAATG AGCGGTGGGC CCGTCCTCCA GCCGTGACCA GCAATGGCAC TCTACACTCG 
CTTAATGCAG ATGCTGAAAG AGAAGAAGGA GAAGAGTTTG AAGACAACAT GGATGTTTTT 
GATGACAGCA GCTCCAGCCC TTCTGGCACC TTGAGAAATT ATCCACTCAC CTGCAAAGTT 
GTTTATTCCT ATAAGGCTTC TCAACCAGAT GAGTTGACCA TTGAGGAACA TGAGGTGTTA 
GAAGTGATTG AAGATGGAGA TATGGAAGAC TGGGTAAAGG CTCGAAATAA AGTTGGCCAA 
GTGGGTTATG TGCCAGAAAA GTACCTACAA TTTCCCACCT CGAACAGCCT ACTGAGCATG 
CTACAGTCTT TGGCTGCTTT GGACAGTCGG TCACACACAT CCAGCAATTC CACAGAAGCA 
GAACTCGTTT CAGGCAGCCT GAATGGAGAT GCCAGTGTCT GTTTTGTGAA AGCACTTTAT 
GATTATGAGG GCCAGACAGA TGATGAGCTG TCCTTTCCCG AGGGAGCAAT TATTCGCATC 
TTGAACAAAG AAAACCAAGA TGATGATGGC TTCTGGGAAG GGGAGTTCAG TGGTCGAATC 
GGTGTTTTCC CATCGGTGCT AGTGGAAGAG CTCTCAGCCT CCGAGAATGG CGACACTCCA 
TGGACAAGAG AGATCCAGAT CTCTCCATCC CCCAAGCCTC ACACATCCCT GCCTCCACTG 
CCTCTGTATG ACCAGCCACC CAGCAGCCCG TATCCCAGTC CAGATAAGAG GAGCTCCCAG 
TTCTTCCCCC GGTCTCCTTC AGCCAATGAA AACAGCCTTC ATGCTGAATC ACCAGGATTC 
TCACAGGCCT CAAGACAGAC TCCTGACACC TCATATGGCA AGCTGCGGCC TGTTCGGGCG 
GCGCCGCCAC CACCCACACA GAACCACCGG CGGACAACTG AGAAGATGGA GGACGTGGAG 
ATCACACTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fchsd2 ( XM_030242304.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098164.1 Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant X2, mRNA.

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CloneID	OMu89407
Clone ID Related Accession (Same CDS sequence)	XM_030242304.1
Accession Version	XM_030242304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	F-BAR and double SH3 domains protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCTG TTTGGAAATC TTTTCTCGAG GGAACAATGC AGGTAGCCCA GTCTCGGATC 
AATATATGTG AAAACTATAA AAACTTCATT TCTGAACCTG CAAGAGCAGT AAGAAGCTTA 
AAAGAACAGC AACTAAAACG GTGTGTGGAT CAGTTGACAA AGATCCAAAC AGAATTACAG 
GAAACTGTGA AAGATTTAGT GAAAGGCAAA AAGAAGTACT TTGAGACTGA ACAGATGGCT 
CATGCAGTAA GAGAGAAAGC TGACATCGAA GCAAAGTCTA AACTTAGTCT TTTTCAATCA 
AGAATCAGTT TACAGAAAGC AAGTGTGAAG TTAAAAGCCC GGCGATCTGA ATGTAATACC 
AAAGCTACCC ATGCAAGGAA TGATTACCTT CTGACGCTAG CAGCAGCTAA CGCACATCAG 
GACCGCTACT ATCAAACAGA CTTAGTTAAC ATCATGAAGG CTCTTGATGG AAACGTGTAT 
GACCACCTCA AGGATTATTT AATAGCCTTC AGCCGGACCG AGCTAGAAAC TTGCCAAGCT 
ATACAGAATA CATTCCAGTT TTTACTAGAA AACTCCAGCA AGGTGGTACG GGACTATAAC 
CTTCAGCTGT TTCTACAAGA GAATGCTGTA TTTCACAAGC CTCAGCCCTT CCAGTTCCAG 
CCTTGTGACA GTGATACTAG TCGACAATTA GAATCAGAAA CTGGTACCAC AGAGGAACAC 
AGTCTAAATA AGGAGGCTCG GAAATGGGCC ACACGTGTGG CACGTGAGCA CAAAAATATT 
GTTCATCAGC AGCGGGTTCT AAATGAACTA GAATGTCATG GAGTTGCTCT ATCAGAACAG 
AGCCGAGCAG AGCTGGAACA GAAAATAGAT GAAGCAAGAG AAAGTATCCG TAAAGCAGAG 
ATCATTAAAT TGAAAGCTGA AGCCCGGCTA GACCTACTGA AGCAGATTGG GGTTTCTGTA 
GATACGTGGT TAAAGAGTGC CATGAACCAA GTGATGGAGG AACTAGAAAA TGAGCGGTGG 
GCCCGTCCTC CAGCCGTGAC CAGCAATGGC ACTCTACACT CGCTTAATGC AGATGCTGAA 
AGAGAAGAAG GAGAAGAGTT TGAAGACAAC ATGGATGTTT TTGATGACAG CAGCTCCAGC 
CCTTCTGGCA CCTTGAGAAA TTATCCACTC ACCTGCAAAG TTGTTTATTC CTATAAGGCT 
TCTCAACCAG ATGAGTTGAC CATTGAGGAA CATGAGGTGT TAGAAGTGAT TGAAGATGGA 
GATATGGAAG ACTGGGTAAA GGCTCGAAAT AAAGTTGGCC AAGTGGGTTA TGTGCCAGAA 
AAGTACCTAC AATTTCCCAC CTCGAACAGC CTACTGAGCA TGCTACAGTC TTTGGCTGCT 
TTGGACAGTC GGTCACACAC ATCCAGCAAT TCCACAGAAG CAGAACTCGT TTCAGGCAGC 
CTGAATGGAG ATGCCAGTGT CTGTTTTGTG AAAGCACTTT ATGATTATGA GGGCCAGACA 
GATGATGAGC TGTCCTTTCC CGAGGGAGCA ATTATTCGCA TCTTGAACAA AGAAAACCAA 
GATGATGATG GCTTCTGGGA AGGGGAGTTC AGTGGTCGAA TCGGTGTTTT CCCATCGGTG 
CTAGTGGAAG AGCTCTCAGC CTCCGAGAAT GGCGACACTC CATGGACAAG AGAGATCCAG 
ATCTCTCCAT CCCCCAAGCC TCACACATCC CTGCCTCCAC TGCCTCTGTA TGACCAGCCA 
CCCAGCAGCC CGTATCCCAG TCCAGATAAG AGGAGCTCCC AGTTCTTCCC CCGGTCTCCT 
TCAGCCAATG AAAACAGCCT TCATGCTGAA TCACCAGGAT TCTCACAGGC CTCAAGACAG 
ACTCCTGACA CCTCATATGG CAAGCTGCGG CCTGTTCGGG CGGCGCCGCC ACCACCCACA 
CAGAACCACC GGCGGACAAC TGAGAAGATG GAGGACGTGG AGATCACACT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098164.1
CDS	462..2438
Translation	MYPVWKSFLEGTMQVAQSRINICENYKNFISEPARAVRSLKEQQLKRCVDQLTKIQTELQETVKDLVKGKKKYFETEQMAHAVREKADIEAKSKLSLFQSRISLQKASVKLKARRSECNTKATHARNDYLLTLAAANAHQDRYYQTDLVNIMKALDGNVYDHLKDYLIAFSRTELETCQAIQNTFQFLLENSSKVVRDYNLQLFLQENAVFHKPQPFQFQPCDSDTSRQLESETGTTEEHSLNKEARKWATRVAREHKNIVHQQRVLNELECHGVALSEQSRAELEQKIDEARESIRKAEIIKLKAEARLDLLKQIGVSVDTWLKSAMNQVMEELENERWARPPAVTSNGTLHSLNADAEREEGEEFEDNMDVFDDSSSSPSGTLRNYPLTCKVVYSYKASQPDELTIEEHEVLEVIEDGDMEDWVKARNKVGQVGYVPEKYLQFPTSNSLLSMLQSLAALDSRSHTSSNSTEAELVSGSLNGDASVCFVKALYDYEGQTDDELSFPEGAIIRILNKENQDDDGFWEGEFSGRIGVFPSVLVEELSASENGDTPWTREIQISPSPKPHTSLPPLPLYDQPPSSPYPSPDKRSSQFFPRSPSANENSLHAESPGFSQASRQTPDTSYGKLRPVRAAPPPPTQNHRRTTEKMEDVEITLV

Target ORF information:

RefSeq Version	XM_030242304.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus FCH and double SH3 domains 2 (Fchsd2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242304.1

ORF Insert Sequence:

ATGTATCCTG TTTGGAAATC TTTTCTCGAG GGAACAATGC AGGTAGCCCA GTCTCGGATC 
AATATATGTG AAAACTATAA AAACTTCATT TCTGAACCTG CAAGAGCAGT AAGAAGCTTA 
AAAGAACAGC AACTAAAACG GTGTGTGGAT CAGTTGACAA AGATCCAAAC AGAATTACAG 
GAAACTGTGA AAGATTTAGT GAAAGGCAAA AAGAAGTACT TTGAGACTGA ACAGATGGCT 
CATGCAGTAA GAGAGAAAGC TGACATCGAA GCAAAGTCTA AACTTAGTCT TTTTCAATCA 
AGAATCAGTT TACAGAAAGC AAGTGTGAAG TTAAAAGCCC GGCGATCTGA ATGTAATACC 
AAAGCTACCC ATGCAAGGAA TGATTACCTT CTGACGCTAG CAGCAGCTAA CGCACATCAG 
GACCGCTACT ATCAAACAGA CTTAGTTAAC ATCATGAAGG CTCTTGATGG AAACGTGTAT 
GACCACCTCA AGGATTATTT AATAGCCTTC AGCCGGACCG AGCTAGAAAC TTGCCAAGCT 
ATACAGAATA CATTCCAGTT TTTACTAGAA AACTCCAGCA AGGTGGTACG GGACTATAAC 
CTTCAGCTGT TTCTACAAGA GAATGCTGTA TTTCACAAGC CTCAGCCCTT CCAGTTCCAG 
CCTTGTGACA GTGATACTAG TCGACAATTA GAATCAGAAA CTGGTACCAC AGAGGAACAC 
AGTCTAAATA AGGAGGCTCG GAAATGGGCC ACACGTGTGG CACGTGAGCA CAAAAATATT 
GTTCATCAGC AGCGGGTTCT AAATGAACTA GAATGTCATG GAGTTGCTCT ATCAGAACAG 
AGCCGAGCAG AGCTGGAACA GAAAATAGAT GAAGCAAGAG AAAGTATCCG TAAAGCAGAG 
ATCATTAAAT TGAAAGCTGA AGCCCGGCTA GACCTACTGA AGCAGATTGG GGTTTCTGTA 
GATACGTGGT TAAAGAGTGC CATGAACCAA GTGATGGAGG AACTAGAAAA TGAGCGGTGG 
GCCCGTCCTC CAGCCGTGAC CAGCAATGGC ACTCTACACT CGCTTAATGC AGATGCTGAA 
AGAGAAGAAG GAGAAGAGTT TGAAGACAAC ATGGATGTTT TTGATGACAG CAGCTCCAGC 
CCTTCTGGCA CCTTGAGAAA TTATCCACTC ACCTGCAAAG TTGTTTATTC CTATAAGGCT 
TCTCAACCAG ATGAGTTGAC CATTGAGGAA CATGAGGTGT TAGAAGTGAT TGAAGATGGA 
GATATGGAAG ACTGGGTAAA GGCTCGAAAT AAAGTTGGCC AAGTGGGTTA TGTGCCAGAA 
AAGTACCTAC AATTTCCCAC CTCGAACAGC CTACTGAGCA TGCTACAGTC TTTGGCTGCT 
TTGGACAGTC GGTCACACAC ATCCAGCAAT TCCACAGAAG CAGAACTCGT TTCAGGCAGC 
CTGAATGGAG ATGCCAGTGT CTGTTTTGTG AAAGCACTTT ATGATTATGA GGGCCAGACA 
GATGATGAGC TGTCCTTTCC CGAGGGAGCA ATTATTCGCA TCTTGAACAA AGAAAACCAA 
GATGATGATG GCTTCTGGGA AGGGGAGTTC AGTGGTCGAA TCGGTGTTTT CCCATCGGTG 
CTAGTGGAAG AGCTCTCAGC CTCCGAGAAT GGCGACACTC CATGGACAAG AGAGATCCAG 
ATCTCTCCAT CCCCCAAGCC TCACACATCC CTGCCTCCAC TGCCTCTGTA TGACCAGCCA 
CCCAGCAGCC CGTATCCCAG TCCAGATAAG AGGAGCTCCC AGTTCTTCCC CCGGTCTCCT 
TCAGCCAATG AAAACAGCCT TCATGCTGAA TCACCAGGAT TCTCACAGGC CTCAAGACAG 
ACTCCTGACA CCTCATATGG CAAGCTGCGG CCTGTTCGGG CGGCGCCGCC ACCACCCACA 
CAGAACCACC GGCGGACAAC TGAGAAGATG GAGGACGTGG AGATCACACT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Formation of membrane ridges and scallops by the F-BAR protein Nervous Wreck.
Molecular biology of the cell24(15)2406-18(2013 Aug)
Becalska AN,Kelley CF,Berciu C,Stanishneva-Konovalova TB,Fu X,Wang S,Sokolova OS,Nicastro D,Rodal AA

FCHSD1 and FCHSD2 are expressed in hair cell stereocilia and cuticular plate and regulate actin polymerization in vitro.
PloS one8(2)e56516(2013)
Cao H,Yin X,Cao Y,Jin Y,Wang S,Kong Y,Chen Y,Gao J,Heller S,Xu Z

Telencephalic embryonic subtractive sequences: a unique collection of neurodevelopmental genes.
The Journal of neuroscience : the official journal of the Society for Neuroscience25(33)7586-600(2005 Aug)
Bulfone A,Carotenuto P,Faedo A,Aglio V,Garzia L,Bello AM,Basile A,Andr? A,Cocchia M,Guardiola O,Ballabio A,Rubenstein JL,Zollo M

Identification and characterization of human FCHSD1 and FCHSD2 genes in silico.
International journal of molecular medicine13(5)749-54(2004 May)
Katoh M,Katoh M

Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(1)35-48(2003 Feb)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Aizawa H,Yuasa S,Nakajima D,Nagase T,Ohara O,Koga H

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Fchsd2 cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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