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Supt5 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Supt5
Entrez Gene ID 20924
Full Name suppressor of Ty 5
Synonyms AL033283,AL033321,AU019549,Spt5,Supt5h
General protein information
Preferred Names

suppressor of Ty 5

Names

transcription elongation factor SPT5
DRB sensitivity-inducing factor large subunit
DSIF large subunit
suppressor of Ty 5 homolog
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

7

7 A3|7 16.68 cM

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_013676.1 NP_038704.1 transcription elongation factor SPT5
XM_006539702.2 XP_006539765.1 transcription elongation factor SPT5 isoform X1
XM_006539703.2 XP_006539766.1 transcription elongation factor SPT5 isoform X2

Pathways from BioSystems


Homologies


Anopheles gambiae (African malaria mosquito) AgaP_AGAP005021 XP_001231045.2
Caenorhabditis elegans (roundworm) spt-5 NP_502283.1
Xenopus tropicalis (tropical clawed frog) supt5h NP_001123796.1
Canis lupus familiaris (dog) SUPT5H XP_005616644.1
Mus musculus (house mouse) Supt5 NP_038704.1
Gallus gallus (chicken) SUPT5H NP_001026555.1
Neurospora crassa NCU06929 XP_959276.2
SUPT5H XP_001088495.1
Bos taurus (cattle) SUPT5H NP_001076908.2
Rattus norvegicus (Norway rat) Supt5h NP_001100967.2
Schizosaccharomyces pombe (fission yeast) spt5 NP_593191.1
Arabidopsis thaliana (thale cress) AT2G34210 NP_180968.2
Oryza sativa (rice) Os02g0772000 NP_001173159.1
Saccharomyces cerevisiae (baker's yeast) SPT5 NP_013703.1
Eremothecium gossypii AGOS_ADR187W NP_984283.2
Homo sapiens (human) SUPT5H NP_001104490.1
Pan troglodytes (chimpanzee) SUPT5H XP_512652.3
Danio rerio (zebrafish) supt5h NP_571748.2
Drosophila melanogaster (fruit fly) Spt5 NP_652610.1
Kluyveromyces lactis KLLA0B00891g XP_451570.1
Magnaporthe oryzae (rice blast fungus) MGG_12268 XP_003715018.1
Arabidopsis thaliana (thale cress) GTA2 NP_192575.3

Gene Ontology


Bibliography

Related articles in PubMed

Spt5 accumulation at variable genes distinguishes somatic hypermutation in germinal center B cells from ex vivo-activated cells.
Maul RW, Cao Z, Venkataraman L, Giorgetti CA, Press JL, Denizot Y, Du H, Sen R, Gearhart PJ
The Journal of experimental medicine211(11)2297-306(2014 Oct)

Chromatin loop organization of the junb locus in mouse dendritic cells.
Salem T, Gomard T, Court F, Moquet-Torcy G, Brockly F, Forn? T, Piechaczyk M
Nucleic acids research41(19)8908-25(2013 Oct)

E3-ubiquitin ligase Nedd4 determines the fate of AID-associated RNA polymerase II in B cells.
Sun J, Keim CD, Wang J, Kazadi D, Oliver PM, Rabadan R, Basu U
Genes & development27(16)1821-33(2013 Aug)

The DSIF subunits Spt4 and Spt5 have distinct roles at various phases of immunoglobulin class switch recombination.
Stanlie A, Begum NA, Akiyama H, Honjo T
PLoS genetics8(4)e1002675(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spt5 accumulation at variable genes distinguishes somatic hypermutation in germinal center B cells from ex vivo-activated cells.
Title: Spt5 accumulation at variable genes distinguishes somatic hypermutation in germinal center B cells from ex vivo-activated cells.

our findings indicate that Spt4 and Spt5 have essential functions in the DNA repair phase of immunoglobulin class switch recombination.
Title: The DSIF subunits Spt4 and Spt5 have distinct roles at various phases of immunoglobulin class switch recombination.

Study reports that Spt5, a factor normally associated with stalled or paused Pol II, is required for class switch recombination.
Title: Activation-induced cytidine deaminase targets DNA at sites of RNA polymerase II stalling by interaction with Spt5.

The following Supt5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Supt5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu14683 NM_013676.1
Latest version!		 Mus musculus suppressor of Ty 5, DSIF elongation factor subunit (Supt5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $559.30
$799.00
OMu38110 XM_006539702.2
Latest version!		 Mus musculus suppressor of Ty 5, DSIF elongation factor subunit (Supt5), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $559.30
$799.00
OMu38111 XM_006539703.2
Latest version!		 Mus musculus suppressor of Ty 5, DSIF elongation factor subunit (Supt5), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $559.30
$799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu14683
Clone ID Related Accession (Same CDS sequence) NM_013676.1
Accession Version NM_013676.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-06-24
Organism Mus musculus(house mouse)
Product transcription elongation factor SPT5
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC007132.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007132.1, AK145117.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140, SAMN01164141 [ECO:0000348] ##Evidence-Data-END## 

1
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961
1021
1081
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1381
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1501
1561
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1681
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1801
1861
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1981
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2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
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ATGTCGGACA GCGAAGACAG CAACTTCTCC GAGGAGGAGG ACAGCGAGCG CAGCAGCGAG 
GCGGAGGAGG CCGAGGTTGA GGAAGACCAG CGAAGTGCTG CAGGCAGTGA AAAAGAGGAG 
GAGCCTGAGG AAGAGGAGGA GGAAGAGGAA GAATACGATG AGGAAGAAGA GGAGGAAGAT 
GATGATAGGC CCCCCAAGAA ACCACGCCAT GGGGGCTTCA TTTTAGATGA GGCTGATGTA 
GATGATGAAT ATGAAGATGA AGACCAGTGG GAAGATGGAG CTGAAGATAT CCTGGAGAAA 
GAAGAGATTG AAGCCTCCAA TATTGATAAC GTTGTGCTGG ATGAAGACCG CTCTGGGGCT 
CGTCGTCTGC AGAATCTCTG GAGGGACCAG AGAGAAGAAG AACTGGGCGA GTATTACATG 
AAGAAATATG CTAAGTCGTC TGTGGGAGAG ACGGTGTATG GAGGGTCTGA CGAGCTCTCA 
GATGACATCA CTCAGCAGCA GCTGCTCCCA GGAGTCAAGG ACCCCAACCT GTGGACTGTC 
AAGTGTAAGA TTGGGGAGGA ACGAGCCACG GCAATTTCCT TGATGCGGAA ATTCATCGCT 
TACCAGTTCA CAGACACACC CCTGCAGATT AAGTCAGTGG TTGCCCCTGA GCACGTGAAG 
GGCTACATCT ATGTGGAGGC CTACAAACAG ACCCATGTGA AGCAGGCCAT TGAAGGTGTG 
GGCAACCTGC GGCTTGGCTA CTGGAACCAG CAGATGGTGC CTATTAAGGA GATGACCGAT 
GTACTCAAGG TGGTGAAGGA GGTGGCCAAC CTGAAGCCGA AGTCCTGGGT CCGCCTCAAG 
CGAGGCATCT ACAAAGATGA CATTGCTCAG GTGGACTATG TGGAGCCCAG CCAGAACACC 
ATTTCCCTGA AGATGATCCC GCGCATCGAC TACGACCGCA TCAAAGCCCG AATGAGCCTG 
AAAGACTGGT TTGCCAAAAG GAAGAAGTTC AAGCGGCCTC CACAGAGGCT CTTTGATGCA 
GAGAAGATCA GGTCCTTGGG GGGTGATGTT GCCTCTGATG GTGACTTCCT CATCTTTGAG 
GGGAACCGCT ACAGCCGAAA GGGCTTCCTG TTCAAGAGCT TTGCCATGTC TGCTGTGATT 
ACAGAAGGTG TGAAGCCCAC ACTGTCAGAG CTGGAAAAGT TTGAAGATCA GCCTGAGGGC 
ATCGACCTGG AGGTTGTGAC TGAAAGCACA GGGAAGGAGC GGGAACACAA TTTCCAGCCT 
GGGGACAATG TGGAGGTGTG CGAGGGCGAG CTCATCAACC TACAGGGCAA GGTCCTCAGC 
GTGGATGGCA ACAAGATCAC TATCATGCCC AAGCACGAGG ACCTCAAGGA CATGCTTGAG 
TTCCCCGCTC AGGAACTTCG GAAGTACTTC AAGATGGGGG ACCACGTAAA AGTGATTGCT 
GGCCGATTTG AGGGTGACAC AGGTCTCATT GTGCGGGTGG AAGAGAACTT TGTTATTCTC 
TTTTCAGACC TCACCATGCA TGAGTTGAAA GTGCTTCCCC GAGACCTGCA GCTCTGCTCA 
GAGACAGCCT CAGGCGTGGA TGTTGGGGGC CAGCATGAGT GGGGTGAGCT GGTGCAGCTG 
GATCCCCGTA CTGTGGGTGT CATCGTGCGG CTAGAGCGAG AAACCTTCCA GGTTCTCAAC 
ATGCACGGGA AGGTGGTGAC CGTTAGGCAC CAGGCCGTGA CGCAGAAGAA GGACAACCGC 
TTCGCTGTGG CTCTGGACTC GGATCAGAAC AACATCCATG TGAAGGACAT TGTCAAGGTC 
ATCGATGGCC CTCACTCAGG CCGAGAGGGT GAGATCCGCC ATCTGTACCG CAGCTTTGCC 
TTCCTGCACT GCAAGAAGCT GGTGGAGAAC GGCGGCATGT TTGTCTGTAA GGCACGGCAC 
CTGGTGTTGG CTGGGGGCTC AAAGCCCCGG GATGTGACCA ATTTAACTGT TGGTGGCTTC 
ACTCCTATGA GCCCCCGGAT CAGCAGCCCT ATGCACCCCA GTGCTGAAGG TCAGCATGGT 
GGCTTTGGAA GCCCAGGTGG CATGAGCAGG GGTCGAGGGA GAAGAGACAA TGAACTCATA 
GGCCAGACTG TGCGTATCTC CCAGGGACCC TACAAAGGCT ACATTGGTGT GGTGAAGGAT 
GCCACAGAGT CCACGGCCAG AGTAGAACTG CATTCTACCT GCCAGACCAT CTCTGTGGAT 
CGCCAGCGGC TCACCACGGT CGACTCCCAG CGTCCAGGTG GCATGACCTC TACATATGGA 
CGGACTCCCA TGTATGGCTC TCAGACACCC ATGTATGGCT CTGGCTCCCG AACACCTATG 
TATGGCTCCC AGACACCTTT GCAGGATGGC AGCCGCACTC CACATTATGG CTCACAGACA 
CCCCTGCATG ATGGTAGCCG CACTCCTGCT CAGAGTGGGG CCTGGGACCC CAACAACCCA 
AACACGCCTT CCAGGGCTGA GGAGGAATAC GAATATGCCT TTGACGACGA ACCCACCCCA 
TCCCCACAAG CATATGGGGG AACCCCGAAT CCCCAAACAC CCGGCTACCC AGACCCATCA 
TCCCCCCAGG TCAATCCACA GTACAATCCA CAGACACCAG GGACGCCAGC CATGTACAAC 
ACAGACCAGT TCTCCCCCTA CGCTGCCCCC TCTCCACAGG GGTCTTACCA GCCCAGCCCC 
AGCCCCCAGA GCTACCACCA AGTGGCACCA AGTCCAGCAG GCTATCAGAA CACCCATTCC 
CCAGCCAGCT ATCACCCGAC ACCGTCACCT ATGGCCTATC AGGCTAGTCC CAGCCCAAGC 
CCTGTTGGCT ACAGTCCAAT GACCCCTGGA GCACCTTCTC CTGGAGGTTA CAATCCCCAC 
ACACCGGGCT CAGGCATTGA GCAGAACTCT AGCGACTGGG TGACCACTGA TATCCAGGTG 
AAGGTTCGAG ACACCTATCT GGACACACAA ATAGTGGGGC AGACAGGCGT CATCCGCAGT 
GTCACGGGAG GCATGTGCTC CGTGTACCTG AAGGACAGTG AGAAGGTGGT CAGCATCTCC 
AGTGAACATC TGGAGCCCAT CACGCCCACC AAGAACAACA AGGTGAAGGT GATTCTGGGT 
GAGGACCGAG AGGCTACCGG GGTCCTACTG AGCATTGATG GTGAGGATGG CATCATCCGC 
ATGGACCTAG AGGACCAGCA GATCAAGATT CTAAACCTCC GCTTCCTAGG GAAGCTCCTG 
GAAGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_038704.1
CDS35..3283
Translation

Target ORF information:

RefSeq Version NM_013676.1
Organism Mus musculus(house mouse)
Definition Mus musculus suppressor of Ty 5, DSIF elongation factor subunit (Supt5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_013676.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCGGACA GCGAAGACAG CAACTTCTCC GAGGAGGAGG ACAGCGAGCG CAGCAGCGAG 
GCGGAGGAGG CCGAGGTTGA GGAAGACCAG CGAAGTGCTG CAGGCAGTGA AAAAGAGGAG 
GAGCCTGAGG AAGAGGAGGA GGAAGAGGAA GAATACGATG AGGAAGAAGA GGAGGAAGAT 
GATGATAGGC CCCCCAAGAA ACCACGCCAT GGGGGCTTCA TTTTAGATGA GGCTGATGTA 
GATGATGAAT ATGAAGATGA AGACCAGTGG GAAGATGGAG CTGAAGATAT CCTGGAGAAA 
GAAGAGATTG AAGCCTCCAA TATTGATAAC GTTGTGCTGG ATGAAGACCG CTCTGGGGCT 
CGTCGTCTGC AGAATCTCTG GAGGGACCAG AGAGAAGAAG AACTGGGCGA GTATTACATG 
AAGAAATATG CTAAGTCGTC TGTGGGAGAG ACGGTGTATG GAGGGTCTGA CGAGCTCTCA 
GATGACATCA CTCAGCAGCA GCTGCTCCCA GGAGTCAAGG ACCCCAACCT GTGGACTGTC 
AAGTGTAAGA TTGGGGAGGA ACGAGCCACG GCAATTTCCT TGATGCGGAA ATTCATCGCT 
TACCAGTTCA CAGACACACC CCTGCAGATT AAGTCAGTGG TTGCCCCTGA GCACGTGAAG 
GGCTACATCT ATGTGGAGGC CTACAAACAG ACCCATGTGA AGCAGGCCAT TGAAGGTGTG 
GGCAACCTGC GGCTTGGCTA CTGGAACCAG CAGATGGTGC CTATTAAGGA GATGACCGAT 
GTACTCAAGG TGGTGAAGGA GGTGGCCAAC CTGAAGCCGA AGTCCTGGGT CCGCCTCAAG 
CGAGGCATCT ACAAAGATGA CATTGCTCAG GTGGACTATG TGGAGCCCAG CCAGAACACC 
ATTTCCCTGA AGATGATCCC GCGCATCGAC TACGACCGCA TCAAAGCCCG AATGAGCCTG 
AAAGACTGGT TTGCCAAAAG GAAGAAGTTC AAGCGGCCTC CACAGAGGCT CTTTGATGCA 
GAGAAGATCA GGTCCTTGGG GGGTGATGTT GCCTCTGATG GTGACTTCCT CATCTTTGAG 
GGGAACCGCT ACAGCCGAAA GGGCTTCCTG TTCAAGAGCT TTGCCATGTC TGCTGTGATT 
ACAGAAGGTG TGAAGCCCAC ACTGTCAGAG CTGGAAAAGT TTGAAGATCA GCCTGAGGGC 
ATCGACCTGG AGGTTGTGAC TGAAAGCACA GGGAAGGAGC GGGAACACAA TTTCCAGCCT 
GGGGACAATG TGGAGGTGTG CGAGGGCGAG CTCATCAACC TACAGGGCAA GGTCCTCAGC 
GTGGATGGCA ACAAGATCAC TATCATGCCC AAGCACGAGG ACCTCAAGGA CATGCTTGAG 
TTCCCCGCTC AGGAACTTCG GAAGTACTTC AAGATGGGGG ACCACGTAAA AGTGATTGCT 
GGCCGATTTG AGGGTGACAC AGGTCTCATT GTGCGGGTGG AAGAGAACTT TGTTATTCTC 
TTTTCAGACC TCACCATGCA TGAGTTGAAA GTGCTTCCCC GAGACCTGCA GCTCTGCTCA 
GAGACAGCCT CAGGCGTGGA TGTTGGGGGC CAGCATGAGT GGGGTGAGCT GGTGCAGCTG 
GATCCCCGTA CTGTGGGTGT CATCGTGCGG CTAGAGCGAG AAACCTTCCA GGTTCTCAAC 
ATGCACGGGA AGGTGGTGAC CGTTAGGCAC CAGGCCGTGA CGCAGAAGAA GGACAACCGC 
TTCGCTGTGG CTCTGGACTC GGATCAGAAC AACATCCATG TGAAGGACAT TGTCAAGGTC 
ATCGATGGCC CTCACTCAGG CCGAGAGGGT GAGATCCGCC ATCTGTACCG CAGCTTTGCC 
TTCCTGCACT GCAAGAAGCT GGTGGAGAAC GGCGGCATGT TTGTCTGTAA GGCACGGCAC 
CTGGTGTTGG CTGGGGGCTC AAAGCCCCGG GATGTGACCA ATTTAACTGT TGGTGGCTTC 
ACTCCTATGA GCCCCCGGAT CAGCAGCCCT ATGCACCCCA GTGCTGAAGG TCAGCATGGT 
GGCTTTGGAA GCCCAGGTGG CATGAGCAGG GGTCGAGGGA GAAGAGACAA TGAACTCATA 
GGCCAGACTG TGCGTATCTC CCAGGGACCC TACAAAGGCT ACATTGGTGT GGTGAAGGAT 
GCCACAGAGT CCACGGCCAG AGTAGAACTG CATTCTACCT GCCAGACCAT CTCTGTGGAT 
CGCCAGCGGC TCACCACGGT CGACTCCCAG CGTCCAGGTG GCATGACCTC TACATATGGA 
CGGACTCCCA TGTATGGCTC TCAGACACCC ATGTATGGCT CTGGCTCCCG AACACCTATG 
TATGGCTCCC AGACACCTTT GCAGGATGGC AGCCGCACTC CACATTATGG CTCACAGACA 
CCCCTGCATG ATGGTAGCCG CACTCCTGCT CAGAGTGGGG CCTGGGACCC CAACAACCCA 
AACACGCCTT CCAGGGCTGA GGAGGAATAC GAATATGCCT TTGACGACGA ACCCACCCCA 
TCCCCACAAG CATATGGGGG AACCCCGAAT CCCCAAACAC CCGGCTACCC AGACCCATCA 
TCCCCCCAGG TCAATCCACA GTACAATCCA CAGACACCAG GGACGCCAGC CATGTACAAC 
ACAGACCAGT TCTCCCCCTA CGCTGCCCCC TCTCCACAGG GGTCTTACCA GCCCAGCCCC 
AGCCCCCAGA GCTACCACCA AGTGGCACCA AGTCCAGCAG GCTATCAGAA CACCCATTCC 
CCAGCCAGCT ATCACCCGAC ACCGTCACCT ATGGCCTATC AGGCTAGTCC CAGCCCAAGC 
CCTGTTGGCT ACAGTCCAAT GACCCCTGGA GCACCTTCTC CTGGAGGTTA CAATCCCCAC 
ACACCGGGCT CAGGCATTGA GCAGAACTCT AGCGACTGGG TGACCACTGA TATCCAGGTG 
AAGGTTCGAG ACACCTATCT GGACACACAA ATAGTGGGGC AGACAGGCGT CATCCGCAGT 
GTCACGGGAG GCATGTGCTC CGTGTACCTG AAGGACAGTG AGAAGGTGGT CAGCATCTCC 
AGTGAACATC TGGAGCCCAT CACGCCCACC AAGAACAACA AGGTGAAGGT GATTCTGGGT 
GAGGACCGAG AGGCTACCGG GGTCCTACTG AGCATTGATG GTGAGGATGG CATCATCCGC 
ATGGACCTAG AGGACCAGCA GATCAAGATT CTAAACCTCC GCTTCCTAGG GAAGCTCCTG 
GAAGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu38110
Clone ID Related Accession (Same CDS sequence) XM_006539702.2
Accession Version XM_006539702.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product transcription elongation factor SPT5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006539702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
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181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGTCGGACA GCGAAGACAG CAACTTCTCC GAGGAGGAGG ACAGCGAGCG CAGCAGCGAG 
GCGGAGGAGG CCGAGGTTGA GGAAGACCAG CGAAGTGCTG CAGGCAGTGA AAAAGAGGAG 
GAGCCTGAGG AAGAGGAGGA GGAAGAGGAA GAATACGATG AGGAAGAAGA GGAGGAAGAT 
GATGATAGGC CCCCCAAGAA ACCACGCCAT GGGGGCTTCA TTTTAGATGA GGCTGATGTA 
GATGATGAAT ATGAAGATGA AGACCAGTGG GAAGATGGAG CTGAAGATAT CCTGGAGAAA 
GCCTCCAATA TTGATAACGT TGTGCTGGAT GAAGACCGCT CTGGGGCTCG TCGTCTGCAG 
AATCTCTGGA GGGACCAGAG AGAAGAAGAA CTGGGCGAGT ATTACATGAA GAAATATGCT 
AAGTCGTCTG TGGGAGAGAC GGTGTATGGA GGGTCTGACG AGCTCTCAGA TGACATCACT 
CAGCAGCAGC TGCTCCCAGG AGTCAAGGAC CCCAACCTGT GGACTGTCAA GTGTAAGATT 
GGGGAGGAAC GAGCCACGGC AATTTCCTTG ATGCGGAAAT TCATCGCTTA CCAGTTCACA 
GACACACCCC TGCAGATTAA GTCAGTGGTT GCCCCTGAGC ACGTGAAGGG CTACATCTAT 
GTGGAGGCCT ACAAACAGAC CCATGTGAAG CAGGCCATTG AAGGTGTGGG CAACCTGCGG 
CTTGGCTACT GGAACCAGCA GATGGTGCCT ATTAAGGAGA TGACCGATGT ACTCAAGGTG 
GTGAAGGAGG TGGCCAACCT GAAGCCGAAG TCCTGGGTCC GCCTCAAGCG AGGCATCTAC 
AAAGATGACA TTGCTCAGGT GGACTATGTG GAGCCCAGCC AGAACACCAT TTCCCTGAAG 
ATGATCCCGC GCATCGACTA CGACCGCATC AAAGCCCGAA TGAGCCTGAA AGACTGGTTT 
GCCAAAAGGA AGAAGTTCAA GCGGCCTCCA CAGAGGCTCT TTGATGCAGA GAAGATCAGG 
TCCTTGGGGG GTGATGTTGC CTCTGATGGT GACTTCCTCA TCTTTGAGGG GAACCGCTAC 
AGCCGAAAGG GCTTCCTGTT CAAGAGCTTT GCCATGTCTG CTGTGATTAC AGAAGGTGTG 
AAGCCCACAC TGTCAGAGCT GGAAAAGTTT GAAGATCAGC CTGAGGGCAT CGACCTGGAG 
GTTGTGACTG AAAGCACAGG GAAGGAGCGG GAACACAATT TCCAGCCTGG GGACAATGTG 
GAGGTGTGCG AGGGCGAGCT CATCAACCTA CAGGGCAAGG TCCTCAGCGT GGATGGCAAC 
AAGATCACTA TCATGCCCAA GCACGAGGAC CTCAAGGACA TGCTTGAGTT CCCCGCTCAG 
GAACTTCGGA AGTACTTCAA GATGGGGGAC CACGTAAAAG TGATTGCTGG CCGATTTGAG 
GGTGACACAG GTCTCATTGT GCGGGTGGAA GAGAACTTTG TTATTCTCTT TTCAGACCTC 
ACCATGCATG AGTTGAAAGT GCTTCCCCGA GACCTGCAGC TCTGCTCAGA GACAGCCTCA 
GGCGTGGATG TTGGGGGCCA GCATGAGTGG GGTGAGCTGG TGCAGCTGGA TCCCCGTACT 
GTGGGTGTCA TCGTGCGGCT AGAGCGAGAA ACCTTCCAGG TTCTCAACAT GCACGGGAAG 
GTGGTGACCG TTAGGCACCA GGCCGTGACG CAGAAGAAGG ACAACCGCTT CGCTGTGGCT 
CTGGACTCGG ATCAGAACAA CATCCATGTG AAGGACATTG TCAAGGTCAT CGATGGCCCT 
CACTCAGGCC GAGAGGGTGA GATCCGCCAT CTGTACCGCA GCTTTGCCTT CCTGCACTGC 
AAGAAGCTGG TGGAGAACGG CGGCATGTTT GTCTGTAAGG CACGGCACCT GGTGTTGGCT 
GGGGGCTCAA AGCCCCGGGA TGTGACCAAT TTAACTGTTG GTGGCTTCAC TCCTATGAGC 
CCCCGGATCA GCAGCCCTAT GCACCCCAGT GCTGAAGGTC AGCATGGTGG CTTTGGAAGC 
CCAGGTGGCA TGAGCAGGGG TCGAGGGAGA AGAGACAATG AACTCATAGG CCAGACTGTG 
CGTATCTCCC AGGGACCCTA CAAAGGCTAC ATTGGTGTGG TGAAGGATGC CACAGAGTCC 
ACGGCCAGAG TAGAACTGCA TTCTACCTGC CAGACCATCT CTGTGGATCG CCAGCGGCTC 
ACCACGGTCG ACTCCCAGCG TCCAGGTGGC ATGACCTCTA CATATGGACG GACTCCCATG 
TATGGCTCTC AGACACCCAT GTATGGCTCT GGCTCCCGAA CACCTATGTA TGGCTCCCAG 
ACACCTTTGC AGGATGGCAG CCGCACTCCA CATTATGGCT CACAGACACC CCTGCATGAT 
GGTAGCCGCA CTCCTGCTCA GAGTGGGGCC TGGGACCCCA ACAACCCAAA CACGCCTTCC 
AGGGCTGAGG AGGAATACGA ATATGCCTTT GACGACGAAC CCACCCCATC CCCACAAGCA 
TATGGGGGAA CCCCGAATCC CCAAACACCC GGCTACCCAG ACCCATCATC CCCCCAGGTC 
AATCCACAGT ACAATCCACA GACACCAGGG ACGCCAGCCA TGTACAACAC AGACCAGTTC 
TCCCCCTACG CTGCCCCCTC TCCACAGGGG TCTTACCAGC CCAGCCCCAG CCCCCAGAGC 
TACCACCAAG TGGCACCAAG TCCAGCAGGC TATCAGAACA CCCATTCCCC AGCCAGCTAT 
CACCCGACAC CGTCACCTAT GGCCTATCAG GCTAGTCCCA GCCCAAGCCC TGTTGGCTAC 
AGTCCAATGA CCCCTGGAGC ACCTTCTCCT GGAGGTTACA ATCCCCACAC ACCGGGCTCA 
GGCATTGAGC AGAACTCTAG CGACTGGGTG ACCACTGATA TCCAGGTGAA GGTTCGAGAC 
ACCTATCTGG ACACACAAAT AGTGGGGCAG ACAGGCGTCA TCCGCAGTGT CACGGGAGGC 
ATGTGCTCCG TGTACCTGAA GGACAGTGAG AAGGTGGTCA GCATCTCCAG TGAACATCTG 
GAGCCCATCA CGCCCACCAA GAACAACAAG GTGAAGGTGA TTCTGGGTGA GGACCGAGAG 
GCTACCGGGG TCCTACTGAG CATTGATGGT GAGGATGGCA TCATCCGCAT GGACCTAGAG 
GACCAGCAGA TCAAGATTCT AAACCTCCGC TTCCTAGGGA AGCTCCTGGA AGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539765.1
CDS120..3356
Translation

Target ORF information:

RefSeq Version XM_006539702.2
Organism Mus musculus(house mouse)
Definition Mus musculus suppressor of Ty 5, DSIF elongation factor subunit (Supt5), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539702.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGTCGGACA GCGAAGACAG CAACTTCTCC GAGGAGGAGG ACAGCGAGCG CAGCAGCGAG 
GCGGAGGAGG CCGAGGTTGA GGAAGACCAG CGAAGTGCTG CAGGCAGTGA AAAAGAGGAG 
GAGCCTGAGG AAGAGGAGGA GGAAGAGGAA GAATACGATG AGGAAGAAGA GGAGGAAGAT 
GATGATAGGC CCCCCAAGAA ACCACGCCAT GGGGGCTTCA TTTTAGATGA GGCTGATGTA 
GATGATGAAT ATGAAGATGA AGACCAGTGG GAAGATGGAG CTGAAGATAT CCTGGAGAAA 
GCCTCCAATA TTGATAACGT TGTGCTGGAT GAAGACCGCT CTGGGGCTCG TCGTCTGCAG 
AATCTCTGGA GGGACCAGAG AGAAGAAGAA CTGGGCGAGT ATTACATGAA GAAATATGCT 
AAGTCGTCTG TGGGAGAGAC GGTGTATGGA GGGTCTGACG AGCTCTCAGA TGACATCACT 
CAGCAGCAGC TGCTCCCAGG AGTCAAGGAC CCCAACCTGT GGACTGTCAA GTGTAAGATT 
GGGGAGGAAC GAGCCACGGC AATTTCCTTG ATGCGGAAAT TCATCGCTTA CCAGTTCACA 
GACACACCCC TGCAGATTAA GTCAGTGGTT GCCCCTGAGC ACGTGAAGGG CTACATCTAT 
GTGGAGGCCT ACAAACAGAC CCATGTGAAG CAGGCCATTG AAGGTGTGGG CAACCTGCGG 
CTTGGCTACT GGAACCAGCA GATGGTGCCT ATTAAGGAGA TGACCGATGT ACTCAAGGTG 
GTGAAGGAGG TGGCCAACCT GAAGCCGAAG TCCTGGGTCC GCCTCAAGCG AGGCATCTAC 
AAAGATGACA TTGCTCAGGT GGACTATGTG GAGCCCAGCC AGAACACCAT TTCCCTGAAG 
ATGATCCCGC GCATCGACTA CGACCGCATC AAAGCCCGAA TGAGCCTGAA AGACTGGTTT 
GCCAAAAGGA AGAAGTTCAA GCGGCCTCCA CAGAGGCTCT TTGATGCAGA GAAGATCAGG 
TCCTTGGGGG GTGATGTTGC CTCTGATGGT GACTTCCTCA TCTTTGAGGG GAACCGCTAC 
AGCCGAAAGG GCTTCCTGTT CAAGAGCTTT GCCATGTCTG CTGTGATTAC AGAAGGTGTG 
AAGCCCACAC TGTCAGAGCT GGAAAAGTTT GAAGATCAGC CTGAGGGCAT CGACCTGGAG 
GTTGTGACTG AAAGCACAGG GAAGGAGCGG GAACACAATT TCCAGCCTGG GGACAATGTG 
GAGGTGTGCG AGGGCGAGCT CATCAACCTA CAGGGCAAGG TCCTCAGCGT GGATGGCAAC 
AAGATCACTA TCATGCCCAA GCACGAGGAC CTCAAGGACA TGCTTGAGTT CCCCGCTCAG 
GAACTTCGGA AGTACTTCAA GATGGGGGAC CACGTAAAAG TGATTGCTGG CCGATTTGAG 
GGTGACACAG GTCTCATTGT GCGGGTGGAA GAGAACTTTG TTATTCTCTT TTCAGACCTC 
ACCATGCATG AGTTGAAAGT GCTTCCCCGA GACCTGCAGC TCTGCTCAGA GACAGCCTCA 
GGCGTGGATG TTGGGGGCCA GCATGAGTGG GGTGAGCTGG TGCAGCTGGA TCCCCGTACT 
GTGGGTGTCA TCGTGCGGCT AGAGCGAGAA ACCTTCCAGG TTCTCAACAT GCACGGGAAG 
GTGGTGACCG TTAGGCACCA GGCCGTGACG CAGAAGAAGG ACAACCGCTT CGCTGTGGCT 
CTGGACTCGG ATCAGAACAA CATCCATGTG AAGGACATTG TCAAGGTCAT CGATGGCCCT 
CACTCAGGCC GAGAGGGTGA GATCCGCCAT CTGTACCGCA GCTTTGCCTT CCTGCACTGC 
AAGAAGCTGG TGGAGAACGG CGGCATGTTT GTCTGTAAGG CACGGCACCT GGTGTTGGCT 
GGGGGCTCAA AGCCCCGGGA TGTGACCAAT TTAACTGTTG GTGGCTTCAC TCCTATGAGC 
CCCCGGATCA GCAGCCCTAT GCACCCCAGT GCTGAAGGTC AGCATGGTGG CTTTGGAAGC 
CCAGGTGGCA TGAGCAGGGG TCGAGGGAGA AGAGACAATG AACTCATAGG CCAGACTGTG 
CGTATCTCCC AGGGACCCTA CAAAGGCTAC ATTGGTGTGG TGAAGGATGC CACAGAGTCC 
ACGGCCAGAG TAGAACTGCA TTCTACCTGC CAGACCATCT CTGTGGATCG CCAGCGGCTC 
ACCACGGTCG ACTCCCAGCG TCCAGGTGGC ATGACCTCTA CATATGGACG GACTCCCATG 
TATGGCTCTC AGACACCCAT GTATGGCTCT GGCTCCCGAA CACCTATGTA TGGCTCCCAG 
ACACCTTTGC AGGATGGCAG CCGCACTCCA CATTATGGCT CACAGACACC CCTGCATGAT 
GGTAGCCGCA CTCCTGCTCA GAGTGGGGCC TGGGACCCCA ACAACCCAAA CACGCCTTCC 
AGGGCTGAGG AGGAATACGA ATATGCCTTT GACGACGAAC CCACCCCATC CCCACAAGCA 
TATGGGGGAA CCCCGAATCC CCAAACACCC GGCTACCCAG ACCCATCATC CCCCCAGGTC 
AATCCACAGT ACAATCCACA GACACCAGGG ACGCCAGCCA TGTACAACAC AGACCAGTTC 
TCCCCCTACG CTGCCCCCTC TCCACAGGGG TCTTACCAGC CCAGCCCCAG CCCCCAGAGC 
TACCACCAAG TGGCACCAAG TCCAGCAGGC TATCAGAACA CCCATTCCCC AGCCAGCTAT 
CACCCGACAC CGTCACCTAT GGCCTATCAG GCTAGTCCCA GCCCAAGCCC TGTTGGCTAC 
AGTCCAATGA CCCCTGGAGC ACCTTCTCCT GGAGGTTACA ATCCCCACAC ACCGGGCTCA 
GGCATTGAGC AGAACTCTAG CGACTGGGTG ACCACTGATA TCCAGGTGAA GGTTCGAGAC 
ACCTATCTGG ACACACAAAT AGTGGGGCAG ACAGGCGTCA TCCGCAGTGT CACGGGAGGC 
ATGTGCTCCG TGTACCTGAA GGACAGTGAG AAGGTGGTCA GCATCTCCAG TGAACATCTG 
GAGCCCATCA CGCCCACCAA GAACAACAAG GTGAAGGTGA TTCTGGGTGA GGACCGAGAG 
GCTACCGGGG TCCTACTGAG CATTGATGGT GAGGATGGCA TCATCCGCAT GGACCTAGAG 
GACCAGCAGA TCAAGATTCT AAACCTCCGC TTCCTAGGGA AGCTCCTGGA AGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu38111
Clone ID Related Accession (Same CDS sequence) XM_006539703.2
Accession Version XM_006539703.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product transcription elongation factor SPT5 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006539703.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGTCGGACA GCGAAGACAG CAACTTCTCC GAGGAGGAGG ACAGCGAGCG CAGCAGCGAG 
GCGGAGGAGG CCGAGGTTGA GGAAGACCAG CGAAGTGCTG CAGGCAGTGA AAAAGAGGAG 
GAGCCTGAGG AAGAGGAGGA GGAAGAGGAA GAATACGATG AGGAAGAAGA GGAGGAAGAT 
GATGATAGGC CCCCCAAGAA ACCACGCCAT GGGGGCTTCA TTTTAGATGA GGCTGATGTA 
GATGATGAAT ATGAAGATGA AGACCAGTGG GAAGATGGAG CTGAAGATAT CCTGGAGAAA 
GAAGAGATTG AAGCCTCCAA TATTGATAAC GTTGTGCTGG ATGAAGACCG CTCTGGGGCT 
CGTCGTCTGC AGAATCTCTG GAGGGACCAG AGAGAAGAAG AACTGGGCGA GTATTACATG 
AAGAAATATG CTAAGTCGTC TGTGGGAGAG ACGGTGTATG GAGGGTCTGA CGAGCTCTCA 
GATGACATCA CTCAGCAGCA GCTGCTCCCA GGAGTCAAGG ACCCCAACCT GTGGACTGTC 
AAGTGTAAGA TTGGGGAGGA ACGAGCCACG GCAATTTCCT TGATGCGGAA ATTCATCGCT 
TACCAGTTCA CAGACACACC CCTGCAGATT AAGTCAGTGG TTGCCCCTGA GCACGTGAAG 
GGCTACATCT ATGTGGAGGC CTACAAACAG ACCCATGTGA AGCAGGCCAT TGAAGGTGTG 
GGCAACCTGC GGCTTGGCTA CTGGAACCAG CAGATGGTGC CTATTAAGGA GATGACCGAT 
GTACTCAAGG TGGTGAAGGA GGTGGCCAAC CTGAAGCCGA AGTCCTGGGT CCGCCTCAAG 
CGAGGCATCT ACAAAGATGA CATTGCTCAG GTGGACTATG TGGAGCCCAG CCAGAACACC 
ATTTCCCTGA AGATGATCCC GCGCATCGAC TACGACCGCA TCAAAGCCCG AATGAGCCTG 
AAAGACTGGT TTGCCAAAAG GAAGAAGTTC AAGCGGCCTC CACAGAGGCT CTTTGATGCA 
GAGAAGATCA GGTCCTTGGG GGGTGATGTT GCCTCTGATG GTGACTTCCT CATCTTTGAG 
GGGAACCGCT ACAGCCGAAA GGGCTTCCTG TTCAAGAGCT TTGCCATGTC TGCTGTGATT 
ACAGAAGGTG TGAAGCCCAC ACTGTCAGAG CTGGAAAAGT TTGAAGATCA GCCTGAGGGC 
ATCGACCTGG AGGTTGTGAC TGAAAGCACA GGGAAGGAGC GGGAACACAA TTTCCAGCCT 
GGGGACAATG TGGAGGTGTG CGAGGGCGAG CTCATCAACC TACAGGGCAA GGTCCTCAGC 
GTGGATGGCA ACAAGATCAC TATCATGCCC AAGCACGAGG ACCTCAAGGA CATGCTTGAG 
TTCCCCGCTC AGGAACTTCG GAAGTACTTC AAGATGGGGG ACCACGTAAA AGTGATTGCT 
GGCCGATTTG AGGGTGACAC AGGTCTCATT GTGCGGGTGG AAGAGAACTT TGTTATTCTC 
TTTTCAGACC TCACCATGCA TGAGTTGAAA GTGCTTCCCC GAGACCTGCA GCTCTGCTCA 
GAGACAGCCT CAGGCGTGGA TGTTGGGGGC CAGCATGAGT GGGGTGAGCT GGTGCAGCTG 
GATCCCCGTA CTGTGGGTGT CATCGTGCGG CTAGAGCGAG AAACCTTCCA GGTTCTCAAC 
ATGCACGGGA AGGTGGTGAC CGTTAGGCAC CAGGCCGTGA CGCAGAAGAA GGACAACCGC 
TTCGCTGTGG CTCTGGACTC GGATCAGAAC AACATCCATG TGAAGGACAT TGTCAAGGTC 
ATCGATGGCC CTCACTCAGG CCGAGAGGGT GAGATCCGCC ATCTGTACCG CAGCTTTGCC 
TTCCTGCACT GCAAGAAGCT GGTGGAGAAC GGCGGCATGT TTGTCTGTAA GGCACGGCAC 
CTGGTGTTGG CTGGGGGCTC AAAGCCCCGG GATGTGACCA ATTTAACTGT TGGTGGCTTC 
ACTCCTATGA GCCCCCGGAT CAGCAGCCCT ATGCACCCCA GTGCTGAAGG TCAGCATGGT 
GGCTTTGGAA GCCCAGGTGG CATGAGCAGG GGTCGAGGGA GAAGAGACAA TGAACTCATA 
GGCCAGACTG TGCGTATCTC CCAGGGACCC TACAAAGGCT ACATTGGTGT GGTGAAGGAT 
GCCACAGAGT CCACGGCCAG AGTAGAACTG CATTCTACCT GCCAGACCAT CTCTGTGGAT 
CGCCAGCGGC TCACCACGGT CGACTCCCAG CGTCCAGGTG GCATGACCTC TACATATGGA 
CGGACTCCCA TGTATGGCTC TCAGACACCC ATGTATGGCT CTGGCTCCCG AACACCTATG 
TATGGCTCCC AGACACCTTT GCAGGATGGC AGCCGCACTC CACATTATGG CTCACAGACA 
CCCCTGCATG ATGGTAGCCG CACTCCTGCT CAGAGTGGGG CCTGGGACCC CAACAACCCA 
AACACGCCTT CCAGGTACAA CACAGACCAG TTCTCCCCCT ACGCTGCCCC CTCTCCACAG 
GGGTCTTACC AGCCCAGCCC CAGCCCCCAG AGCTACCACC AAGTGGCACC AAGTCCAGCA 
GGCTATCAGA ACACCCATTC CCCAGCCAGC TATCACCCGA CACCGTCACC TATGGCCTAT 
CAGGCTAGTC CCAGCCCAAG CCCTGTTGGC TACAGTCCAA TGACCCCTGG AGCACCTTCT 
CCTGGAGGTT ACAATCCCCA CACACCGGGC TCAGGCATTG AGCAGAACTC TAGCGACTGG 
GTGACCACTG ATATCCAGGT GAAGGTTCGA GACACCTATC TGGACACACA AATAGTGGGG 
CAGACAGGCG TCATCCGCAG TGTCACGGGA GGCATGTGCT CCGTGTACCT GAAGGACAGT 
GAGAAGGTGG TCAGCATCTC CAGTGAACAT CTGGAGCCCA TCACGCCCAC CAAGAACAAC 
AAGGTGAAGG TGATTCTGGG TGAGGACCGA GAGGCTACCG GGGTCCTACT GAGCATTGAT 
GGTGAGGATG GCATCATCCG CATGGACCTA GAGGACCAGC AGATCAAGAT TCTAAACCTC 
CGCTTCCTAG GGAAGCTCCT GGAAGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539766.1
CDS120..3209
Translation

Target ORF information:

RefSeq Version XM_006539703.2
Organism Mus musculus(house mouse)
Definition Mus musculus suppressor of Ty 5, DSIF elongation factor subunit (Supt5), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539703.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGTCGGACA GCGAAGACAG CAACTTCTCC GAGGAGGAGG ACAGCGAGCG CAGCAGCGAG 
GCGGAGGAGG CCGAGGTTGA GGAAGACCAG CGAAGTGCTG CAGGCAGTGA AAAAGAGGAG 
GAGCCTGAGG AAGAGGAGGA GGAAGAGGAA GAATACGATG AGGAAGAAGA GGAGGAAGAT 
GATGATAGGC CCCCCAAGAA ACCACGCCAT GGGGGCTTCA TTTTAGATGA GGCTGATGTA 
GATGATGAAT ATGAAGATGA AGACCAGTGG GAAGATGGAG CTGAAGATAT CCTGGAGAAA 
GAAGAGATTG AAGCCTCCAA TATTGATAAC GTTGTGCTGG ATGAAGACCG CTCTGGGGCT 
CGTCGTCTGC AGAATCTCTG GAGGGACCAG AGAGAAGAAG AACTGGGCGA GTATTACATG 
AAGAAATATG CTAAGTCGTC TGTGGGAGAG ACGGTGTATG GAGGGTCTGA CGAGCTCTCA 
GATGACATCA CTCAGCAGCA GCTGCTCCCA GGAGTCAAGG ACCCCAACCT GTGGACTGTC 
AAGTGTAAGA TTGGGGAGGA ACGAGCCACG GCAATTTCCT TGATGCGGAA ATTCATCGCT 
TACCAGTTCA CAGACACACC CCTGCAGATT AAGTCAGTGG TTGCCCCTGA GCACGTGAAG 
GGCTACATCT ATGTGGAGGC CTACAAACAG ACCCATGTGA AGCAGGCCAT TGAAGGTGTG 
GGCAACCTGC GGCTTGGCTA CTGGAACCAG CAGATGGTGC CTATTAAGGA GATGACCGAT 
GTACTCAAGG TGGTGAAGGA GGTGGCCAAC CTGAAGCCGA AGTCCTGGGT CCGCCTCAAG 
CGAGGCATCT ACAAAGATGA CATTGCTCAG GTGGACTATG TGGAGCCCAG CCAGAACACC 
ATTTCCCTGA AGATGATCCC GCGCATCGAC TACGACCGCA TCAAAGCCCG AATGAGCCTG 
AAAGACTGGT TTGCCAAAAG GAAGAAGTTC AAGCGGCCTC CACAGAGGCT CTTTGATGCA 
GAGAAGATCA GGTCCTTGGG GGGTGATGTT GCCTCTGATG GTGACTTCCT CATCTTTGAG 
GGGAACCGCT ACAGCCGAAA GGGCTTCCTG TTCAAGAGCT TTGCCATGTC TGCTGTGATT 
ACAGAAGGTG TGAAGCCCAC ACTGTCAGAG CTGGAAAAGT TTGAAGATCA GCCTGAGGGC 
ATCGACCTGG AGGTTGTGAC TGAAAGCACA GGGAAGGAGC GGGAACACAA TTTCCAGCCT 
GGGGACAATG TGGAGGTGTG CGAGGGCGAG CTCATCAACC TACAGGGCAA GGTCCTCAGC 
GTGGATGGCA ACAAGATCAC TATCATGCCC AAGCACGAGG ACCTCAAGGA CATGCTTGAG 
TTCCCCGCTC AGGAACTTCG GAAGTACTTC AAGATGGGGG ACCACGTAAA AGTGATTGCT 
GGCCGATTTG AGGGTGACAC AGGTCTCATT GTGCGGGTGG AAGAGAACTT TGTTATTCTC 
TTTTCAGACC TCACCATGCA TGAGTTGAAA GTGCTTCCCC GAGACCTGCA GCTCTGCTCA 
GAGACAGCCT CAGGCGTGGA TGTTGGGGGC CAGCATGAGT GGGGTGAGCT GGTGCAGCTG 
GATCCCCGTA CTGTGGGTGT CATCGTGCGG CTAGAGCGAG AAACCTTCCA GGTTCTCAAC 
ATGCACGGGA AGGTGGTGAC CGTTAGGCAC CAGGCCGTGA CGCAGAAGAA GGACAACCGC 
TTCGCTGTGG CTCTGGACTC GGATCAGAAC AACATCCATG TGAAGGACAT TGTCAAGGTC 
ATCGATGGCC CTCACTCAGG CCGAGAGGGT GAGATCCGCC ATCTGTACCG CAGCTTTGCC 
TTCCTGCACT GCAAGAAGCT GGTGGAGAAC GGCGGCATGT TTGTCTGTAA GGCACGGCAC 
CTGGTGTTGG CTGGGGGCTC AAAGCCCCGG GATGTGACCA ATTTAACTGT TGGTGGCTTC 
ACTCCTATGA GCCCCCGGAT CAGCAGCCCT ATGCACCCCA GTGCTGAAGG TCAGCATGGT 
GGCTTTGGAA GCCCAGGTGG CATGAGCAGG GGTCGAGGGA GAAGAGACAA TGAACTCATA 
GGCCAGACTG TGCGTATCTC CCAGGGACCC TACAAAGGCT ACATTGGTGT GGTGAAGGAT 
GCCACAGAGT CCACGGCCAG AGTAGAACTG CATTCTACCT GCCAGACCAT CTCTGTGGAT 
CGCCAGCGGC TCACCACGGT CGACTCCCAG CGTCCAGGTG GCATGACCTC TACATATGGA 
CGGACTCCCA TGTATGGCTC TCAGACACCC ATGTATGGCT CTGGCTCCCG AACACCTATG 
TATGGCTCCC AGACACCTTT GCAGGATGGC AGCCGCACTC CACATTATGG CTCACAGACA 
CCCCTGCATG ATGGTAGCCG CACTCCTGCT CAGAGTGGGG CCTGGGACCC CAACAACCCA 
AACACGCCTT CCAGGTACAA CACAGACCAG TTCTCCCCCT ACGCTGCCCC CTCTCCACAG 
GGGTCTTACC AGCCCAGCCC CAGCCCCCAG AGCTACCACC AAGTGGCACC AAGTCCAGCA 
GGCTATCAGA ACACCCATTC CCCAGCCAGC TATCACCCGA CACCGTCACC TATGGCCTAT 
CAGGCTAGTC CCAGCCCAAG CCCTGTTGGC TACAGTCCAA TGACCCCTGG AGCACCTTCT 
CCTGGAGGTT ACAATCCCCA CACACCGGGC TCAGGCATTG AGCAGAACTC TAGCGACTGG 
GTGACCACTG ATATCCAGGT GAAGGTTCGA GACACCTATC TGGACACACA AATAGTGGGG 
CAGACAGGCG TCATCCGCAG TGTCACGGGA GGCATGTGCT CCGTGTACCT GAAGGACAGT 
GAGAAGGTGG TCAGCATCTC CAGTGAACAT CTGGAGCCCA TCACGCCCAC CAAGAACAAC 
AAGGTGAAGG TGATTCTGGG TGAGGACCGA GAGGCTACCG GGGTCCTACT GAGCATTGAT 
GGTGAGGATG GCATCATCCG CATGGACCTA GAGGACCAGC AGATCAAGAT TCTAAACCTC 
CGCTTCCTAG GGAAGCTCCT GGAAGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Spt5 accumulation at variable genes distinguishes somatic hypermutation in germinal center B cells from ex vivo-activated cells.
The Journal of experimental medicine211(11)2297-306(2014 Oct)
Maul RW,Cao Z,Venkataraman L,Giorgetti CA,Press JL,Denizot Y,Du H,Sen R,Gearhart PJ


book

Chromatin loop organization of the junb locus in mouse dendritic cells.
Nucleic acids research41(19)8908-25(2013 Oct)
Salem T,Gomard T,Court F,Moquet-Torcy G,Brockly F,Forn? T,Piechaczyk M


book

E3-ubiquitin ligase Nedd4 determines the fate of AID-associated RNA polymerase II in B cells.
Genes & development27(16)1821-33(2013 Aug)
Sun J,Keim CD,Wang J,Kazadi D,Oliver PM,Rabadan R,Basu U


book

The DSIF subunits Spt4 and Spt5 have distinct roles at various phases of immunoglobulin class switch recombination.
PLoS genetics8(4)e1002675(2012)
Stanlie A,Begum NA,Akiyama H,Honjo T