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Med22 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Med22
Entrez Gene ID 20933
Full Name mediator complex subunit 22
Synonyms AW212655,AW558812,Surf-5,Surf5
General protein information
Preferred Names

mediator complex subunit 22

Names

mediator of RNA polymerase II transcription subunit 22
surfeit gene 5
surfeit locus protein 5
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

2

2 A3|2 19.09 cM

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006497829.1 XP_006497892.1 mediator of RNA polymerase II transcription subunit 22 isoform X1
NM_001033908.1 NP_001029080.1 mediator of RNA polymerase II transcription subunit 22 isoform 2
NM_011513.2 NP_035643.1 mediator of RNA polymerase II transcription subunit 22 isoform 1

Pathways from BioSystems


Homologies


Homo sapiens (human) MED22 NP_598395.1
Bos taurus (cattle) MED22 NP_001029914.1
Mus musculus (house mouse) Med22 NP_001029080.1
Pan troglodytes (chimpanzee) MED22 XP_528453.2
Gallus gallus (chicken) MED22 NP_001039295.1
Xenopus tropicalis (tropical clawed frog) med22 NP_001120562.1
Canis lupus familiaris (dog) MED22 XP_005625201.1
Rattus norvegicus (Norway rat) Med22 NP_001071147.1
Rattus norvegicus (Norway rat) LOC100911917 XP_003749478.1
Anopheles gambiae (African malaria mosquito) MED22_ANOGA XP_313084.3
Danio rerio (zebrafish) med22 NP_001002144.1
MED22 XP_001101391.1
Drosophila melanogaster (fruit fly) MED22 NP_569870.1

Gene Ontology


Bibliography

Related articles in PubMed

A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2.
Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW
The Journal of biological chemistry279(7)5846-51(2004 Feb)

Constitutive knockout of Surf1 is associated with high embryonic lethality, mitochondrial disease and cytochrome c oxidase deficiency in mice.
Agostino A, Invernizzi F, Tiveron C, Fagiolari G, Prelle A, Lamantea E, Giavazzi A, Battaglia G, Tatangelo L, Tiranti V, Zeviani M
Human molecular genetics12(4)399-413(2003 Feb)

The human homologue of the mouse Surf5 gene encodes multiple alternatively spliced transcripts.
Angiolillo A, Russo G, Porcellini A, Smaldone S, D'Alessandro F, Pietropaolo C
Gene284(1-2)169-78(2002 Feb)

Gene expression in the developing mouse retina by EST sequencing and microarray analysis.
Mu X, Zhao S, Pershad R, Hsieh TF, Scarpa A, Wang SW, White RA, Beremand PD, Thomas TL, Gan L, Klein WH
Nucleic acids research29(24)4983-93(2001 Dec)

The Surf-6 gene of the mouse surfeit locus encodes a novel nucleolar protein.
Magoulas C, Fried M
DNA and cell biology15(4)305-16(1996 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Med22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu12758 NM_001033908.1
Latest version!		 Mus musculus mediator complex subunit 22 (Med22), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OMu29407 XM_006497829.1
Latest version!		 Mus musculus mediator complex subunit 22 (Med22), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OMu08310 NM_011513.2
Latest version!		 Mus musculus mediator complex subunit 22 (Med22), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu12758
Clone ID Related Accession (Same CDS sequence) NM_001033908.1
Accession Version NM_001033908.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 603bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-18
Organism Mus musculus(house mouse)
Product mediator of RNA polymerase II transcription subunit 22 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL773563.12 and BG805496.1. Transcript Variant: This variant (2) uses alternate splice sites in the coding region, compared to variant 1. The resulting isoform (2) is longer and has a unique C-terminus when it is compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: FO719623.1, FO720089.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGCCCAGC AGAGAGCCCT TCCCCAGAGC AAGGAGACGC TGCTGCAGTC ATACAACAAG 
CGGCTCAAAG ACGACATCAA GTCCATCATG GACAACTTCA CCGAGATCAT CAAGACCGCC 
AAGATTGAGG ATGAGACGCA GGTGTCTCGG GCTACTCAGG GCGAACAGGA CAATTATGAG 
ATGCACGTTC GAGCAGCCAA CATCGTTCGA GCTGGTGAGT CACTGATGAA GCTGGTATCC 
GATCTCAAGC AGTTTCTGAT CCTCAACGAC TTCCCGTCTG TGAATGAAGC CATCGACCAG 
CGTAACCAGC AGCTTCGAGC CCTGCAGGAG GAGTGTGACC GCAAGCTCAT CACCCTGCGG 
GACGAGGTCT CCATTGACCT ATATGAGCTG GAGGAGGAGT ACTACTCGTC CAGCTCAAGT 
CTCTGCGAAG CTAATGACCT GCCTCTGTGT GAAGCTTACT GGAGGCTGGA CCTCGACGCA 
GATTCTGCTG ATGGCCTCTC AGCCCCTCTT CTGGCGTCCC CGGAGACTGG TGCTGGTCCC 
TTACAGTCTG CAGCCCCTGT CCACTCCCAT GGTGGTGGCC CTGGCCCTAC AGAACACACC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001029080.1
CDS228..830
Translation

Target ORF information:

RefSeq Version NM_001033908.1
Organism Mus musculus(house mouse)
Definition Mus musculus mediator complex subunit 22 (Med22), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001033908.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCCCAGC AGAGAGCCCT TCCCCAGAGC AAGGAGACGC TGCTGCAGTC ATACAACAAG 
CGGCTCAAAG ACGACATCAA GTCCATCATG GACAACTTCA CCGAGATCAT CAAGACCGCC 
AAGATTGAGG ATGAGACGCA GGTGTCTCGG GCTACTCAGG GCGAACAGGA CAATTATGAG 
ATGCACGTTC GAGCAGCCAA CATCGTTCGA GCTGGTGAGT CACTGATGAA GCTGGTATCC 
GATCTCAAGC AGTTTCTGAT CCTCAACGAC TTCCCGTCTG TGAATGAAGC CATCGACCAG 
CGTAACCAGC AGCTTCGAGC CCTGCAGGAG GAGTGTGACC GCAAGCTCAT CACCCTGCGG 
GACGAGGTCT CCATTGACCT ATATGAGCTG GAGGAGGAGT ACTACTCGTC CAGCTCAAGT 
CTCTGCGAAG CTAATGACCT GCCTCTGTGT GAAGCTTACT GGAGGCTGGA CCTCGACGCA 
GATTCTGCTG ATGGCCTCTC AGCCCCTCTT CTGGCGTCCC CGGAGACTGG TGCTGGTCCC 
TTACAGTCTG CAGCCCCTGT CCACTCCCAT GGTGGTGGCC CTGGCCCTAC AGAACACACC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu29407
Clone ID Related Accession (Same CDS sequence) XM_006497829.1
Accession Version XM_006497829.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 711bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product mediator of RNA polymerase II transcription subunit 22 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCCCAGC AGAGAGCCCT TCCCCAGAGC AAGGAGACGC TGCTGCAGTC ATACAACAAG 
CGGCTCAAAG ACGACATCAA GTCCATCATG GACAACTTCA CCGAGATCAT CAAGACCGCC 
AAGATTGAGG ATGAGACGCA GGTGTCTCGG GCTACTCAGG GCGAACAGGA CAATTATGAG 
ATGCACGTTC GAGCAGCCAA CATCAACGTA AACCAGACTT CCAGGATGCA CTGCTGGCTC 
AGCAGCACGT ACACAGAGCA GGGCCACTGT GTCTGCCTCC TTTTATATTT TACCCTGATT 
ACGATGGTGC AGGTTCGAGC TGGTGAGTCA CTGATGAAGC TGGTATCCGA TCTCAAGCAG 
TTTCTGATCC TCAACGACTT CCCGTCTGTG AATGAAGCCA TCGACCAGCG TAACCAGCAG 
CTTCGAGCCC TGCAGGAGGA GTGTGACCGC AAGCTCATCA CCCTGCGGGA CGAGGTCTCC 
ATTGACCTAT ATGAGCTGGA GGAGGAGTAC TACTCGTCCA GCTCAAGTCT CTGCGAAGCT 
AATGACCTGC CTCTGTGTGA AGCTTACTGG AGGCTGGACC TCGACGCAGA TTCTGCTGAT 
GGCCTCTCAG CCCCTCTTCT GGCGTCCCCG GAGACTGGTG CTGGTCCCTT ACAGTCTGCA 
GCCCCTGTCC ACTCCCATGG TGGTGGCCCT GGCCCTACAG AACACACCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006497892.1
CDS258..968
Translation

Target ORF information:

RefSeq Version XM_006497829.1
Organism Mus musculus(house mouse)
Definition Mus musculus mediator complex subunit 22 (Med22), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006497829.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCCCAGC AGAGAGCCCT TCCCCAGAGC AAGGAGACGC TGCTGCAGTC ATACAACAAG 
CGGCTCAAAG ACGACATCAA GTCCATCATG GACAACTTCA CCGAGATCAT CAAGACCGCC 
AAGATTGAGG ATGAGACGCA GGTGTCTCGG GCTACTCAGG GCGAACAGGA CAATTATGAG 
ATGCACGTTC GAGCAGCCAA CATCAACGTA AACCAGACTT CCAGGATGCA CTGCTGGCTC 
AGCAGCACGT ACACAGAGCA GGGCCACTGT GTCTGCCTCC TTTTATATTT TACCCTGATT 
ACGATGGTGC AGGTTCGAGC TGGTGAGTCA CTGATGAAGC TGGTATCCGA TCTCAAGCAG 
TTTCTGATCC TCAACGACTT CCCGTCTGTG AATGAAGCCA TCGACCAGCG TAACCAGCAG 
CTTCGAGCCC TGCAGGAGGA GTGTGACCGC AAGCTCATCA CCCTGCGGGA CGAGGTCTCC 
ATTGACCTAT ATGAGCTGGA GGAGGAGTAC TACTCGTCCA GCTCAAGTCT CTGCGAAGCT 
AATGACCTGC CTCTGTGTGA AGCTTACTGG AGGCTGGACC TCGACGCAGA TTCTGCTGAT 
GGCCTCTCAG CCCCTCTTCT GGCGTCCCCG GAGACTGGTG CTGGTCCCTT ACAGTCTGCA 
GCCCCTGTCC ACTCCCATGG TGGTGGCCCT GGCCCTACAG AACACACCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08310
Clone ID Related Accession (Same CDS sequence) NM_011513.2
Accession Version NM_011513.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 423bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-18
Organism Mus musculus(house mouse)
Product mediator of RNA polymerase II transcription subunit 22 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL773563.12. On Oct 3, 2005 this sequence version replaced NM_011513.1. Transcript Variant: This variant (1) represents the longer transcript and it encodes the shorter protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: BC018225.1, SRR1660817.91580.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849389 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
ATGGCCCAGC AGAGAGCCCT TCCCCAGAGC AAGGAGACGC TGCTGCAGTC ATACAACAAG 
CGGCTCAAAG ACGACATCAA GTCCATCATG GACAACTTCA CCGAGATCAT CAAGACCGCC 
AAGATTGAGG ATGAGACGCA GGTGTCTCGG GCTACTCAGG GCGAACAGGA CAATTATGAG 
ATGCACGTTC GAGCAGCCAA CATCGTTCGA GCTGGTGAGT CACTGATGAA GCTGGTATCC 
GATCTCAAGC AGTTTCTGAT CCTCAACGAC TTCCCGTCTG TGAATGAAGC CATCGACCAG 
CGTAACCAGC AGCTTCGAGC CCTGCAGGAG GAGTGTGACC GCAAGCTCAT CACCCTGCGG 
GACGAGGTCT CCATTGACCT ATATGAGCTG GAGGAGGAGT ACTACTCGTC CAGGTACAAA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_035643.1
CDS228..650
Translation

Target ORF information:

RefSeq Version NM_011513.2
Organism Mus musculus(house mouse)
Definition Mus musculus mediator complex subunit 22 (Med22), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_011513.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCCCAGC AGAGAGCCCT TCCCCAGAGC AAGGAGACGC TGCTGCAGTC ATACAACAAG 
CGGCTCAAAG ACGACATCAA GTCCATCATG GACAACTTCA CCGAGATCAT CAAGACCGCC 
AAGATTGAGG ATGAGACGCA GGTGTCTCGG GCTACTCAGG GCGAACAGGA CAATTATGAG 
ATGCACGTTC GAGCAGCCAA CATCGTTCGA GCTGGTGAGT CACTGATGAA GCTGGTATCC 
GATCTCAAGC AGTTTCTGAT CCTCAACGAC TTCCCGTCTG TGAATGAAGC CATCGACCAG 
CGTAACCAGC AGCTTCGAGC CCTGCAGGAG GAGTGTGACC GCAAGCTCAT CACCCTGCGG 
GACGAGGTCT CCATTGACCT ATATGAGCTG GAGGAGGAGT ACTACTCGTC CAGGTACAAA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2.
The Journal of biological chemistry279(7)5846-51(2004 Feb)
Tomomori-Sato C,Sato S,Parmely TJ,Banks CA,Sorokina I,Florens L,Zybailov B,Washburn MP,Brower CS,Conaway RC,Conaway JW


book

Constitutive knockout of Surf1 is associated with high embryonic lethality, mitochondrial disease and cytochrome c oxidase deficiency in mice.
Human molecular genetics12(4)399-413(2003 Feb)
Agostino A,Invernizzi F,Tiveron C,Fagiolari G,Prelle A,Lamantea E,Giavazzi A,Battaglia G,Tatangelo L,Tiranti V,Zeviani M


book

The human homologue of the mouse Surf5 gene encodes multiple alternatively spliced transcripts.
Gene284(1-2)169-78(2002 Feb)
Angiolillo A,Russo G,Porcellini A,Smaldone S,D'Alessandro F,Pietropaolo C


book

Gene expression in the developing mouse retina by EST sequencing and microarray analysis.
Nucleic acids research29(24)4983-93(2001 Dec)
Mu X,Zhao S,Pershad R,Hsieh TF,Scarpa A,Wang SW,White RA,Beremand PD,Thomas TL,Gan L,Klein WH


book

The Surf-6 gene of the mouse surfeit locus encodes a novel nucleolar protein.
DNA and cell biology15(4)305-16(1996 Apr)
Magoulas C,Fried M