| Gene Symbol | Astl |
| Entrez Gene ID | 215095 |
| Full Name | astacin-like metalloendopeptidase (M12 family) |
| Synonyms | 7420700F21,C87576,Sas1b |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Mus musculus(house mouse) |
| Genome |
gRNAs
The following Astl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Astl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu14391 | |
| Clone ID Related Accession (Same CDS sequence) | NM_172539.3 | |
| Accession Version | NM_172539.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-06-15 | |
| Organism | Mus musculus(house mouse) | |
| Product | astacin-like metalloendopeptidase isoform b | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845368.6, AK033037.1 and DV662582.1. On Mar 21, 2014 this sequence version replaced NM_172539.2. Transcript Variant: This variant (2) contains an alternate 5' terminal exon, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AK033037.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGGAGCAC CCTCAGCATC CAGATGTTCT GGAGTCTGCA GTACCAGTGT TCCAGAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_766127.1 |
| CDS | 256..1500 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_172539.3 |
1 | ATGGGAGCAC CCTCAGCATC CAGATGTTCT GGAGTCTGCA GTACCAGTGT TCCAGAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu16784 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001291003.1 | |
| Accession Version | NM_001291003.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Mus musculus(house mouse) | |
| Product | astacin-like metalloendopeptidase isoform a precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV649054.1, BC064729.1 and DV662582.1. On Mar 21, 2014 this sequence version replaced XM_006499122.1. Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC064729.1, AJ537599.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGGTATCA TGGGAAGCCT GTGGCCTTGG ATATTAACTA TGCTCTCCTT GCTAGGTTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001277932.1 |
| CDS | 62..1369 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001291003.1 |
1 | ATGGGTATCA TGGGAAGCCT GTGGCCTTGG ATATTAACTA TGCTCTCCTT GCTAGGTTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Intracellular activation of ovastacin mediates pre-fertilization hardening of the zona pellucida. |
A Unique Egg Cortical Granule Localization Motif Is Required for Ovastacin Sequestration to Prevent Premature ZP2 Cleavage and Ensure Female Fertility in Mice. |
Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy. |
Astacin-like metallo-endopeptidase is dynamically expressed in embryonic stem cells and embryonic epithelium during morphogenesis. |
