Usp12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Usp12
Entrez Gene ID	22217
Full Name	ubiquitin specific peptidase 12
Synonyms	Ubh1
General protein information	Preferred Names ubiquitin specific peptidase 12 Names ubiquitin carboxyl-terminal hydrolase 12 deubiquitinating enzyme 12 ubiquitin specific protease 12 ubiquitin thioesterase 12 ubiquitin thiolesterase 12 ubiquitin-hydrolyzing enzyme 1 ubiquitin-specific-processing protease 12
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030254365.1	XP_030110225.1	ubiquitin carboxyl-terminal hydrolase 12 isoform X1
XM_006504819.3	XP_006504882.1	ubiquitin carboxyl-terminal hydrolase 12 isoform X2
XM_006504818.2	XP_006504881.1	ubiquitin carboxyl-terminal hydrolase 12 isoform X1
NM_011669.3	NP_035799.1	ubiquitin carboxyl-terminal hydrolase 12

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP12	NP_872294.2
Bos taurus (cattle)	USP12	NP_001091528.1
Rattus norvegicus (Norway rat)	Usp12	NP_001160048.1
Schizosaccharomyces pombe (fission yeast)	ubp9	NP_596207.1
Pan troglodytes (chimpanzee)	USP12	XP_001135418.2
Canis lupus familiaris (dog)	USP12	XP_543159.2
Mus musculus (house mouse)	Usp12	NP_035799.1
Gallus gallus (chicken)	USP12	XP_004938833.1
Oryza sativa (rice)	Os03g0190800	NP_001049228.1
Danio rerio (zebrafish)	usp12a	NP_001077025.1
Xenopus tropicalis (tropical clawed frog)	LOC100494517	XP_002941584.2
Neurospora crassa	NCU08378	XP_965281.2
	LOC721611	XP_001117796.2
Magnaporthe oryzae (rice blast fungus)	MGG_09907	XP_003710004.1

Gene Ontology
Bibliography

Related articles in PubMed

Deubiquitinase USP12 promotes LPS induced macrophage responses through inhibition of I?B?.
Nayak TKS, Alamuru-Yellapragada NP, Parsa KVL
Biochemical and biophysical research communications483(1)69-74(2017 01)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.
Jahan AS, Lestra M, Swee LK, Fan Y, Lamers MM, Tafesse FG, Theile CS, Spooner E, Bruzzone R, Ploegh HL, Sanyal S
Proceedings of the National Academy of Sciences of the United States of America113(6)E705-14(2016 Feb)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mechanistic analysis suggest that USP12 may be required for the activation of NFkappaB pathway as knockdown of USP12 reduced the inhibitory phosphorylation of IkappaBalpha, a well characterized inhibitor of NFkappaB nuclear translocation.
Title: Deubiquitinase USP12 promotes LPS induced macrophage responses through inhibition of I?B?.

Product has deubiquitinating enzyme activity and sequence analysis showed 98.3% amino acid identity with human UBH1.
Title: Molecular cloning and complete cDNA sequence of UBH1 in mouse testis.

The following Usp12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu36391	XM_006504818.2 Latest version!	Mus musculus ubiquitin specific peptidase 12 (Usp12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu36392	XM_006504819.3 Latest version!	Mus musculus ubiquitin specific peptidase 12 (Usp12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu08580	NM_011669.3 Latest version!	Mus musculus ubiquitin specific peptidase 12 (Usp12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu36391	XM_030254365.1 Latest version!	Mus musculus ubiquitin specific peptidase 12 (Usp12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp12 ( XM_006504818.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504881.1 Mus musculus ubiquitin specific peptidase 12 (Usp12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu36391
Clone ID Related Accession (Same CDS sequence)	XM_030254365.1 , XM_006504818.2
Accession Version	XM_006504818.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504818.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTCA ATACAATTGC TGATATTCTA 
CAAGAAGAAA GAAAACAGGA GAAGCAAAAT GGCCGATTAC GGAACGGAGA CGTGGACAAC 
GAGGATAACA ACAGCACACC AGACCCAACC TGGGTCCACG AGATCTTTCA GGGAACATTA 
ACTAATGAAA CCAGGTGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAACACA TCAATTACTC ACTGCTTAAG AGGGTTCAGT 
AACACAGAAA CTCTGTGCAG TGAATATAAG TACTACTGTG AAGAGTGCCG GAGCAAGCAG 
GAAGCACACA AGCGGATGAA GGTTAAGAAA CTGCCCCTGA TCCTGGCTCT GCACCTGAAG 
AGGTTTAAGT ATATGGACCA GCTTCACCGG TACACGAAGC TTTCTTATCG CGTGGTCTTT 
CCTTTAGAGC TCCGTCTGTT CAACACGTCA GGAGATGCGA CCAACCCTGA CAGAATGTAT 
GACCTGGTCG CCGTTGTGGT TCACTGTGGA AGTGGTCCCA ACCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT CTGGCTGTTG TTTGATGATG ACATTGTAGA AAAAATCGAT 
GCCCAAGCTA TTGAAGAATT CTACGGATTG ACATCTGACA TCTCCAAGAA CTCGGAGTCT 
GGCTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504881.1
CDS	100..852
Translation	MQQDAHEFLNYLLNTIADILQEERKQEKQNGRLRNGDVDNEDNNSTPDPTWVHEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMKVKKLPLILALHLKRFKYMDQLHRYTKLSYRVVFPLELRLFNTSGDATNPDRMYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRD

Target ORF information:

RefSeq Version	XM_006504818.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 12 (Usp12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504818.2

ORF Insert Sequence:

ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTCA ATACAATTGC TGATATTCTA 
CAAGAAGAAA GAAAACAGGA GAAGCAAAAT GGCCGATTAC GGAACGGAGA CGTGGACAAC 
GAGGATAACA ACAGCACACC AGACCCAACC TGGGTCCACG AGATCTTTCA GGGAACATTA 
ACTAATGAAA CCAGGTGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAACACA TCAATTACTC ACTGCTTAAG AGGGTTCAGT 
AACACAGAAA CTCTGTGCAG TGAATATAAG TACTACTGTG AAGAGTGCCG GAGCAAGCAG 
GAAGCACACA AGCGGATGAA GGTTAAGAAA CTGCCCCTGA TCCTGGCTCT GCACCTGAAG 
AGGTTTAAGT ATATGGACCA GCTTCACCGG TACACGAAGC TTTCTTATCG CGTGGTCTTT 
CCTTTAGAGC TCCGTCTGTT CAACACGTCA GGAGATGCGA CCAACCCTGA CAGAATGTAT 
GACCTGGTCG CCGTTGTGGT TCACTGTGGA AGTGGTCCCA ACCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT CTGGCTGTTG TTTGATGATG ACATTGTAGA AAAAATCGAT 
GCCCAAGCTA TTGAAGAATT CTACGGATTG ACATCTGACA TCTCCAAGAA CTCGGAGTCT 
GGCTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp12 ( XM_006504819.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504882.1 Mus musculus ubiquitin specific peptidase 12 (Usp12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu36392
Clone ID Related Accession (Same CDS sequence)	XM_006504819.3
Accession Version	XM_006504819.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504819.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATCC TAATGACAGT CTCCAAATTC GCCTCCATCT GTACCATGGG CGCCAATGCC 
TCGGCATTAG AGAAAGAGAT TGGTCCAGAA CAGTTTCCGG TCAACGAGCA CTATTTTGGA 
TTAGTCAATT TTGGGAATAC CTGCTACTGC AACTCAGTTC TTCAAGCACT TTATTTTTGT 
CGTCCATTTC GGGAAAAAGT TCTAGCATAC AAAAGTCAGC CCAGAAAAAA GGAGAACCTT 
CTCACCTGCC TGGCAGACCT CTTCCACAGC ATAGCCACCC AGAAGAAGAA GGTCGGCGTG 
ATCCCGCCCA AGAAGTTCAT CACAAGGTTG CGGAAAGAAA ACGAGCTGTT TGACAACTAC 
ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTCA ATACAATTGC TGATATTCTA 
CAAGAAGAAA GAAAACAGGA GAAGCAAAAT GGCCGATTAC GGAACGGAGA CGTGGACAAC 
GAGGATAACA ACAGCACACC AGACCCAACC TGGGTCCACG AGATCTTTCA GGGAACATTA 
ACTAATGAAA CCAGGTGTCT TACTTGTGAA ACTGCCAAGT CAGTCCCACC CAGTTCTCCA 
ACCTCGGTGG CTCGTCCTAC AAGTCCTGGC CTTGAAGATC TCGTCCAGAG CACCTGGGCG 
GTGTCCACAT GGAGCCTGTG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504882.1
CDS	290..976
Translation	MEILMTVSKFASICTMGANASALEKEIGPEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKVLAYKSQPRKKENLLTCLADLFHSIATQKKKVGVIPPKKFITRLRKENELFDNYMQQDAHEFLNYLLNTIADILQEERKQEKQNGRLRNGDVDNEDNNSTPDPTWVHEIFQGTLTNETRCLTCETAKSVPPSSPTSVARPTSPGLEDLVQSTWAVSTWSLCF

Target ORF information:

RefSeq Version	XM_006504819.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 12 (Usp12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504819.3

ORF Insert Sequence:

ATGGAAATCC TAATGACAGT CTCCAAATTC GCCTCCATCT GTACCATGGG CGCCAATGCC 
TCGGCATTAG AGAAAGAGAT TGGTCCAGAA CAGTTTCCGG TCAACGAGCA CTATTTTGGA 
TTAGTCAATT TTGGGAATAC CTGCTACTGC AACTCAGTTC TTCAAGCACT TTATTTTTGT 
CGTCCATTTC GGGAAAAAGT TCTAGCATAC AAAAGTCAGC CCAGAAAAAA GGAGAACCTT 
CTCACCTGCC TGGCAGACCT CTTCCACAGC ATAGCCACCC AGAAGAAGAA GGTCGGCGTG 
ATCCCGCCCA AGAAGTTCAT CACAAGGTTG CGGAAAGAAA ACGAGCTGTT TGACAACTAC 
ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTCA ATACAATTGC TGATATTCTA 
CAAGAAGAAA GAAAACAGGA GAAGCAAAAT GGCCGATTAC GGAACGGAGA CGTGGACAAC 
GAGGATAACA ACAGCACACC AGACCCAACC TGGGTCCACG AGATCTTTCA GGGAACATTA 
ACTAATGAAA CCAGGTGTCT TACTTGTGAA ACTGCCAAGT CAGTCCCACC CAGTTCTCCA 
ACCTCGGTGG CTCGTCCTAC AAGTCCTGGC CTTGAAGATC TCGTCCAGAG CACCTGGGCG 
GTGTCCACAT GGAGCCTGTG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp12 ( NM_011669.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035799.1 Mus musculus ubiquitin specific peptidase 12 (Usp12), mRNA.

Price & Availability↑

CloneID	OMu08580
Clone ID Related Accession (Same CDS sequence)	NM_011669.3
Accession Version	NM_011669.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC124828.14 and AC127589.4. On Nov 17, 2006 this sequence version replaced NM_011669.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC068136.1, AK035629.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

ATGGAAATCC TAATGACAGT CTCCAAATTC GCCTCCATCT GTACCATGGG CGCCAATGCC 
TCGGCATTAG AGAAAGAGAT TGGTCCAGAA CAGTTTCCGG TCAACGAGCA CTATTTTGGA 
TTAGTCAATT TTGGGAATAC CTGCTACTGC AACTCAGTTC TTCAAGCACT TTATTTTTGT 
CGTCCATTTC GGGAAAAAGT TCTAGCATAC AAAAGTCAGC CCAGAAAAAA GGAGAACCTT 
CTCACCTGCC TGGCAGACCT CTTCCACAGC ATAGCCACCC AGAAGAAGAA GGTCGGCGTG 
ATCCCGCCCA AGAAGTTCAT CACAAGGTTG CGGAAAGAAA ACGAGCTGTT TGACAACTAC 
ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTCA ATACAATTGC TGATATTCTA 
CAAGAAGAAA GAAAACAGGA GAAGCAAAAT GGCCGATTAC GGAACGGAGA CGTGGACAAC 
GAGGATAACA ACAGCACACC AGACCCAACC TGGGTCCACG AGATCTTTCA GGGAACATTA 
ACTAATGAAA CCAGGTGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAACACA TCAATTACTC ACTGCTTAAG AGGGTTCAGT 
AACACAGAAA CTCTGTGCAG TGAATATAAG TACTACTGTG AAGAGTGCCG GAGCAAGCAG 
GAAGCACACA AGCGGATGAA GGTTAAGAAA CTGCCCCTGA TCCTGGCTCT GCACCTGAAG 
AGGTTTAAGT ATATGGACCA GCTTCACCGG TACACGAAGC TTTCTTATCG CGTGGTCTTT 
CCTTTAGAGC TCCGTCTGTT CAACACGTCA GGAGATGCGA CCAACCCTGA CAGAATGTAT 
GACCTGGTCG CCGTTGTGGT TCACTGTGGA AGTGGTCCCA ACCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT CTGGCTGTTG TTTGATGATG ACATTGTAGA AAAAATCGAT 
GCCCAAGCTA TTGAAGAATT CTACGGATTG ACATCTGACA TCTCCAAGAA CTCGGAGTCT 
GGCTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035799.1
CDS	295..1407
Translation	MEILMTVSKFASICTMGANASALEKEIGPEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKVLAYKSQPRKKENLLTCLADLFHSIATQKKKVGVIPPKKFITRLRKENELFDNYMQQDAHEFLNYLLNTIADILQEERKQEKQNGRLRNGDVDNEDNNSTPDPTWVHEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMKVKKLPLILALHLKRFKYMDQLHRYTKLSYRVVFPLELRLFNTSGDATNPDRMYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRD

Target ORF information:

RefSeq Version	NM_011669.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 12 (Usp12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011669.3

ORF Insert Sequence:

ATGGAAATCC TAATGACAGT CTCCAAATTC GCCTCCATCT GTACCATGGG CGCCAATGCC 
TCGGCATTAG AGAAAGAGAT TGGTCCAGAA CAGTTTCCGG TCAACGAGCA CTATTTTGGA 
TTAGTCAATT TTGGGAATAC CTGCTACTGC AACTCAGTTC TTCAAGCACT TTATTTTTGT 
CGTCCATTTC GGGAAAAAGT TCTAGCATAC AAAAGTCAGC CCAGAAAAAA GGAGAACCTT 
CTCACCTGCC TGGCAGACCT CTTCCACAGC ATAGCCACCC AGAAGAAGAA GGTCGGCGTG 
ATCCCGCCCA AGAAGTTCAT CACAAGGTTG CGGAAAGAAA ACGAGCTGTT TGACAACTAC 
ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTCA ATACAATTGC TGATATTCTA 
CAAGAAGAAA GAAAACAGGA GAAGCAAAAT GGCCGATTAC GGAACGGAGA CGTGGACAAC 
GAGGATAACA ACAGCACACC AGACCCAACC TGGGTCCACG AGATCTTTCA GGGAACATTA 
ACTAATGAAA CCAGGTGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAACACA TCAATTACTC ACTGCTTAAG AGGGTTCAGT 
AACACAGAAA CTCTGTGCAG TGAATATAAG TACTACTGTG AAGAGTGCCG GAGCAAGCAG 
GAAGCACACA AGCGGATGAA GGTTAAGAAA CTGCCCCTGA TCCTGGCTCT GCACCTGAAG 
AGGTTTAAGT ATATGGACCA GCTTCACCGG TACACGAAGC TTTCTTATCG CGTGGTCTTT 
CCTTTAGAGC TCCGTCTGTT CAACACGTCA GGAGATGCGA CCAACCCTGA CAGAATGTAT 
GACCTGGTCG CCGTTGTGGT TCACTGTGGA AGTGGTCCCA ACCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT CTGGCTGTTG TTTGATGATG ACATTGTAGA AAAAATCGAT 
GCCCAAGCTA TTGAAGAATT CTACGGATTG ACATCTGACA TCTCCAAGAA CTCGGAGTCT 
GGCTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp12 ( XM_030254365.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110225.1 Mus musculus ubiquitin specific peptidase 12 (Usp12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu36391
Clone ID Related Accession (Same CDS sequence)	XM_030254365.1 , XM_006504818.2
Accession Version	XM_030254365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTCA ATACAATTGC TGATATTCTA 
CAAGAAGAAA GAAAACAGGA GAAGCAAAAT GGCCGATTAC GGAACGGAGA CGTGGACAAC 
GAGGATAACA ACAGCACACC AGACCCAACC TGGGTCCACG AGATCTTTCA GGGAACATTA 
ACTAATGAAA CCAGGTGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAACACA TCAATTACTC ACTGCTTAAG AGGGTTCAGT 
AACACAGAAA CTCTGTGCAG TGAATATAAG TACTACTGTG AAGAGTGCCG GAGCAAGCAG 
GAAGCACACA AGCGGATGAA GGTTAAGAAA CTGCCCCTGA TCCTGGCTCT GCACCTGAAG 
AGGTTTAAGT ATATGGACCA GCTTCACCGG TACACGAAGC TTTCTTATCG CGTGGTCTTT 
CCTTTAGAGC TCCGTCTGTT CAACACGTCA GGAGATGCGA CCAACCCTGA CAGAATGTAT 
GACCTGGTCG CCGTTGTGGT TCACTGTGGA AGTGGTCCCA ACCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT CTGGCTGTTG TTTGATGATG ACATTGTAGA AAAAATCGAT 
GCCCAAGCTA TTGAAGAATT CTACGGATTG ACATCTGACA TCTCCAAGAA CTCGGAGTCT 
GGCTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110225.1
CDS	349..1101
Translation	MQQDAHEFLNYLLNTIADILQEERKQEKQNGRLRNGDVDNEDNNSTPDPTWVHEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMKVKKLPLILALHLKRFKYMDQLHRYTKLSYRVVFPLELRLFNTSGDATNPDRMYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRD

Target ORF information:

RefSeq Version	XM_030254365.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 12 (Usp12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254365.1

ORF Insert Sequence:

ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTCA ATACAATTGC TGATATTCTA 
CAAGAAGAAA GAAAACAGGA GAAGCAAAAT GGCCGATTAC GGAACGGAGA CGTGGACAAC 
GAGGATAACA ACAGCACACC AGACCCAACC TGGGTCCACG AGATCTTTCA GGGAACATTA 
ACTAATGAAA CCAGGTGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAACACA TCAATTACTC ACTGCTTAAG AGGGTTCAGT 
AACACAGAAA CTCTGTGCAG TGAATATAAG TACTACTGTG AAGAGTGCCG GAGCAAGCAG 
GAAGCACACA AGCGGATGAA GGTTAAGAAA CTGCCCCTGA TCCTGGCTCT GCACCTGAAG 
AGGTTTAAGT ATATGGACCA GCTTCACCGG TACACGAAGC TTTCTTATCG CGTGGTCTTT 
CCTTTAGAGC TCCGTCTGTT CAACACGTCA GGAGATGCGA CCAACCCTGA CAGAATGTAT 
GACCTGGTCG CCGTTGTGGT TCACTGTGGA AGTGGTCCCA ACCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT CTGGCTGTTG TTTGATGATG ACATTGTAGA AAAAATCGAT 
GCCCAAGCTA TTGAAGAATT CTACGGATTG ACATCTGACA TCTCCAAGAA CTCGGAGTCT 
GGCTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deubiquitinase USP12 promotes LPS induced macrophage responses through inhibition of I?B?.
Biochemical and biophysical research communications483(1)69-74(2017 01)
Nayak TKS,Alamuru-Yellapragada NP,Parsa KVL

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.
Proceedings of the National Academy of Sciences of the United States of America113(6)E705-14(2016 Feb)
Jahan AS,Lestra M,Swee LK,Fan Y,Lamers MM,Tafesse FG,Theile CS,Spooner E,Bruzzone R,Ploegh HL,Sanyal S

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

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Usp12 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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