Unc13b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Unc13b
Entrez Gene ID	22249
Full Name	unc-13 homolog B (C. elegans)
Synonyms	Munc13-1,Munc13-2,Unc13a,Unc13h1,Unc13h2
General protein information	Preferred Names unc-13 homolog B (C. elegans) Names protein unc-13 homolog B UNC-13 homolog 1 munc13 unc-13 homolog A unc13 homolog 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 A5

mRNA and Protein(s)

mRNA	Protein	Name
XM_017320115.1	XP_017175604.1	protein unc-13 homolog B isoform X1
XM_006537788.3	XP_006537851.1	protein unc-13 homolog B isoform X2
XM_006537795.3	XP_006537858.1	protein unc-13 homolog B isoform X8
XM_006537791.4	XP_006537854.1	protein unc-13 homolog B isoform X5
XM_006537795.4	XP_006537858.1	protein unc-13 homolog B isoform X8
XM_017320117.1	XP_017175606.1	protein unc-13 homolog B isoform X9
XM_006537790.3	XP_006537853.1	protein unc-13 homolog B isoform X4
XM_017320118.1	XP_017175607.1	protein unc-13 homolog B isoform X10
XM_017320115.2	XP_017175604.1	protein unc-13 homolog B isoform X1
XM_006537790.4	XP_006537853.1	protein unc-13 homolog B isoform X4
XM_030253406.1	XP_030109266.1	protein unc-13 homolog B isoform X10
NM_001081413.2	NP_001074882.1	protein unc-13 homolog B isoform 2
XM_006537786.3	XP_006537849.1	protein unc-13 homolog B isoform X1
XM_006537791.3	XP_006537854.1	protein unc-13 homolog B isoform X5
XM_017320116.1	XP_017175605.1	protein unc-13 homolog B isoform X6
XM_006537786.4	XP_006537849.1	protein unc-13 homolog B isoform X1
XM_006537794.4	XP_006537857.1	protein unc-13 homolog B isoform X7
XM_017320118.2	XP_017175607.1	protein unc-13 homolog B isoform X10
NM_001310758.1	NP_001297687.1	protein unc-13 homolog B isoform 3
XM_006537789.3	XP_006537852.1	protein unc-13 homolog B isoform X3
XM_006537794.3	XP_006537857.1	protein unc-13 homolog B isoform X7
XM_006537788.4	XP_006537851.1	protein unc-13 homolog B isoform X2
XM_006537789.4	XP_006537852.1	protein unc-13 homolog B isoform X3
XM_017320116.2	XP_017175605.1	protein unc-13 homolog B isoform X6
NM_021468.3	NP_067443.2	protein unc-13 homolog B isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	UNC13B	XP_005626825.1
Mus musculus (house mouse)	Unc13b	NP_067443.2
Gallus gallus (chicken)	LOC100858799	XP_004937184.1
Pan troglodytes (chimpanzee)	UNC13B	XP_519737.2
	UNC13B	XP_001085980.2
Danio rerio (zebrafish)	LOC100329499	XP_002663560.3
Homo sapiens (human)	UNC13B	NP_006368.3
Bos taurus (cattle)	UNC13B	NP_001180168.1
Rattus norvegicus (Norway rat)	Unc13b	NP_001036044.1

Gene Ontology
Bibliography

Related articles in PubMed

Munc13 proteins control regulated exocytosis in mast cells.
Rodarte EM, Ramos MA, Davalos AJ, Moreira DC, Moreno DS, Cardenas EI, Rodarte AI, Petrova Y, Molina S, Rendon LE, Sanchez E, Breaux K, Tortoriello A, Manllo J, Gonzalez EA, Tuvim MJ, Dickey BF, Burns AR, Heidelberger R, Adachi R
The Journal of biological chemistry293(1)345-358(2018 Jan)

Doc2B acts as a calcium sensor for vesicle priming requiring synaptotagmin-1, Munc13-2 and SNAREs.
Houy S, Groffen AJ, Ziomkiewicz I, Verhage M, Pinheiro PS, S?rensen JB
eLife6(2017 Dec)

Phosphatidylinositol 4,5-bisphosphate optical uncaging potentiates exocytosis.
Walter AM, M?ller R, Tawfik B, Wierda KD, Pinheiro PS, Nadler A, McCarthy AW, Ziomkiewicz I, Kruse M, Reither G, Rettig J, Lehmann M, Haucke V, Hille B, Schultz C, S?rensen JB
eLife6(2017 Oct)

Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18.
Lai Y, Choi UB, Leitz J, Rhee HJ, Lee C, Altas B, Zhao M, Pfuetzner RA, Wang AL, Brose N, Rhee J, Brunger AT
Neuron95(3)591-607.e10(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Munc13-2 is recruited to synapses by the AZ protein ELKS1.
Title: ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones.

SIGNIFICANCE STATEMENT: DBA/2J and C57BL/6J mice have been used to understand the genetic mechanisms controlling behaviors related to a number of psychiatric illnesses. However, the fundamental neurobiological mechanisms producing these behavioral characteristics remain unresolved. Here we identify a critical family of presynaptic proteins differentially expressed by these strains that control strain-dependent synaptic ph
Title: Differential Expression of Munc13-2 Produces Unique Synaptic Phenotypes in the Basolateral Amygdala of C57BL/6J and DBA/2J Mice.

Munc13-2 plays a fundamental role in large dense-core vesicle exocytosis, but in contrast to synapses lacking Munc13s, the corresponding chromaffin cells do not exhibit a vesicle docking defect.
Title: Identification of a Munc13-sensitive step in chromaffin cell large dense-core vesicle exocytosis.

betaARs couple to a cAMP/Epac/PLC/Munc13-1/Rab3a/RIM1a-dependent pathway to enhance glutamate release at cerebrocortical nerve terminals.
Title: ?-Adrenergic receptors activate exchange protein directly activated by cAMP (Epac), translocate Munc13-1, and enhance the Rab3A-RIM1? interaction to potentiate glutamate release at cerebrocortical nerve terminals.

Our data establish Munc13-1 as a major presynaptic target of Ca(2+)-Calmodulin signaling and show that the Ca(2+)-Calmodulin-Munc13-1 complex is a pivotal component of the molecular machinery that determines short-term synaptic plasticity characteristics.
Title: Dynamic control of synaptic vesicle replenishment and short-term plasticity by Ca(2+)-calmodulin-Munc13-1 signaling.

The following Unc13b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Unc13b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu32852	XM_017320115.2 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu32852	XM_006537786.4 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu32853	XM_006537788.4 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu32854	XM_006537789.4 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu32855	XM_006537790.4 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu32856	XM_006537791.4 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu32859	XM_006537794.4 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu32860	XM_006537795.4 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu80937	XM_017320116.2 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu80938	XM_017320117.1 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu80939	XM_030253406.1 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu80939	XM_017320118.2 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu02383	NM_001081413.2 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$937.30 $1339.00
OMu12393	NM_021468.3 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$937.30 $1339.00
OMu69012	NM_001310758.1 Latest version!	Mus musculus unc-13 homolog B (Unc13b), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu32860	XM_006537795.3	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu32852	XM_017320115.1	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu32855	XM_006537790.3	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu32856	XM_006537791.3	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu32852	XM_006537786.3	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu80939	XM_017320118.1	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu32859	XM_006537794.3	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu32853	XM_006537788.3	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu32854	XM_006537789.3	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu80937	XM_017320116.1	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Unc13b ( XM_017320115.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175604.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant X2, mRNA.

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CloneID	OMu32852
Clone ID Related Accession (Same CDS sequence)	XM_017320115.1 , XM_017320115.2 , XM_006537786.3 , XM_006537786.4
Accession Version	XM_017320115.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGCAGGAACA ACCTCTAGAG GAACCAGGGC CCAGCATTCG GAACTTGGAT 
TTCTGGCCCA AACTTATCAC ACTCATTGTT TCGATCATAG AGGAGGATAA GAATTCCTAC 
ACACCTGTTC TGAGCCAGTT TCCTCAGGAG TTGAACGTAG GAAAAGTCAG TGCAGAAGTG 
ATGTGGCATC TGTTTGCTCA AGACATGAAA TATGCACTGG AGGAACACGA GAAAGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TATGTGCGCG ATCTGCCTGC CCTCCAGGGG CAAGTGCCTG AGTACCCAGC GTGGTTTGAG 
CAGTTTGTGC TACAGTGGCT AGATGAGAAT GAAGATGTGT CACTGGAATT CCTTCGTGGG 
GCCTTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC CTTGTTTTCC 
TGTTCTGTGG TGGATGTCTT TACACAACTC AACCAGAGCT TTGAGATCAT CCGGAAGTTG 
GAATGTCCAG ACCCCAACAT CCTTGCCCAC TACATGAGAA GGTTTGCTAA GACCATTGGG 
AAAGTGCTGA TGCAGTATGC AGACATCTTA TCAAAGAACT TCCCAGCTTA CTGCACAAAG 
GAGAGACTGC CCTGTATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAAGAGCTG GACTCCGAAG CTGCAGACAG TCTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTTAGCA TGGTGTTTGG AAACAGCTTC 
CAGGTGCGGA TTGATGAATG TGTTCGACAA ATGGCTGACA TCCTGGGCCA AGTTCGGGGC 
ACAGGGAATG CATCCCCTAA CGCCAGGGCC TCGGTGGCTC AGGATGCAGA TAGTGTGCTG 
CGACCTCTCA TGGACTTCCT GGATGGCAAC CTCACGCTGT TTGCCACTGT GTGTGAGAAG 
ACAGTTCTGA AGCGGGTCCT GAAGGAGCTC TGGCGGGTGG TGATGAACAC CATGGAGCGG 
GTTATTGTCC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT AACTGCTGCC 
AAGGAGCTGA GCCAGCTTTC CAAACTCAAG GACCACATGG TACGGGAGGA AACACGGAAT 
CTCACTCCAA AGCAGTGTGC TGTCCTCGAC TTGGCCTTGG ACACCATCAA GCAATACTTC 
CATGCAGGAG GCAATGGTCT GAAGAAAACC TTCCTGGAAA AGAGCCCAGA CCTGCAGTCT 
CTGCGTTATG CCCTCTCTCT GTATACACAG ACTACAGACA CTCTCATCAA GACATTTGTG 
CGCTCACAGA CTGCCCAGGT ACATGATGGG AAAGGTATTA GGTTTACTGC TAACGAGGAC 
ATTCGGCCAG AAAAGGGGGC TGGTGTGGAT GACCCCGTGG GAGAAGTCTC TATTCAGGTG 
GACTTATTTA CACATCCTGG CACTGGGGAG CATAAGGTCA CAGTGAAAGT GGTAGCAGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCTT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGATAA GAAGAGGAAG TTCACAACCA AGTCTAAAAG TAACAACTGG 
ACTCCCAAGT ACAACGAGAC GTTTCACTTC CTCCTGGGAA ATGAAGAGGG TCCAGAGGCC 
TATGAATTGC AGATCTGTGT GAAGGATTAC TGCTTTGCCC GGGAGGATCG TGTGATAGGA 
CTGGCAGTGA TGCCCCTGAG GGATGTGGCA GCTAAGGGCA GCTGTGCCTG CTGGTGCCCA 
CTGGGCCGGA AGATCCATAT GGATGAGACA GGCATGACCA TTCTCCGGAT TCTGTCTCAG 
AGGAGCAATG ACGAGGTAGC CCGAGAGTTC GTGAAGCTCA AATCAGAGTC TCGATCCACA 
GAGGAAGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175604.1
CDS	2569..8523
Translation	MKRLLRESEEEIMLTLGPSSSLSPDKVRAETVCSVKSKSTGPTGSLPEDNFPLPCCDSTTSGERDGDLPQLCSFEQQANNQPSLASTACASGSDSRELSPASITNCSDPSERNQARPILPRGPGQRCRYEQQEPLGDVVEYIIRELQGISRLQSEIAELQQHLNQVQGSVDEVSSCVDSVLSEIEGLHVGSSSLGKVCSGEKAQELHVERSREEAILYLYGLPEHDGENTMELVDNFLAKHLCVNGMQCNRYIQEAYRTGTAPAPRPTVVKLVHLEHRDLILQKSILLQSVGVRVATREEPVWSEGCEDPPKESLSCLQQLQDHSWNSLNQDKPALRLETGNRKQMTEPHQIRTQNQQRGPQASEHQGPRLLPKDGLAKQSDVSKLQEEVKGTSGASWVTSDCCGELSLLHPLEGSSSVLISKEEDCEKLQIFKQGRQEHEACKVTKLQSDCSNSIKDSSCLSLSGPLKADMVNTEDKMLGCEAGLDILSPKQLEDLLADKSRRFATLSPDSTMEEVIIGPETLSNMVHIDLNEEENCTAQVLKDVFDKSSRVLADSQEDEDVEIKFHTTKLSRAIQHFRLALQGVFQKLENNGSISPEDLESNESGSQSENSDRLLWTVSSGGAHDCSVESPASQGSESLLSVVSGGVGISIQGDQTSQAPSNFSLASNNSPFANSLLSFPLAPCLGSETCSRPDSPNQGKLSLEQVCAETIYLNKCINNFKNVLREKRLRQKKLLQELVQTASHLSVEDIPSEGNQEAIQISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_017320115.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320115.2

ORF Insert Sequence:

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGCAGGAACA ACCTCTAGAG GAACCAGGGC CCAGCATTCG GAACTTGGAT 
TTCTGGCCCA AACTTATCAC ACTCATTGTT TCGATCATAG AGGAGGATAA GAATTCCTAC 
ACACCTGTTC TGAGCCAGTT TCCTCAGGAG TTGAACGTAG GAAAAGTCAG TGCAGAAGTG 
ATGTGGCATC TGTTTGCTCA AGACATGAAA TATGCACTGG AGGAACACGA GAAAGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TATGTGCGCG ATCTGCCTGC CCTCCAGGGG CAAGTGCCTG AGTACCCAGC GTGGTTTGAG 
CAGTTTGTGC TACAGTGGCT AGATGAGAAT GAAGATGTGT CACTGGAATT CCTTCGTGGG 
GCCTTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC CTTGTTTTCC 
TGTTCTGTGG TGGATGTCTT TACACAACTC AACCAGAGCT TTGAGATCAT CCGGAAGTTG 
GAATGTCCAG ACCCCAACAT CCTTGCCCAC TACATGAGAA GGTTTGCTAA GACCATTGGG 
AAAGTGCTGA TGCAGTATGC AGACATCTTA TCAAAGAACT TCCCAGCTTA CTGCACAAAG 
GAGAGACTGC CCTGTATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAAGAGCTG GACTCCGAAG CTGCAGACAG TCTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTTAGCA TGGTGTTTGG AAACAGCTTC 
CAGGTGCGGA TTGATGAATG TGTTCGACAA ATGGCTGACA TCCTGGGCCA AGTTCGGGGC 
ACAGGGAATG CATCCCCTAA CGCCAGGGCC TCGGTGGCTC AGGATGCAGA TAGTGTGCTG 
CGACCTCTCA TGGACTTCCT GGATGGCAAC CTCACGCTGT TTGCCACTGT GTGTGAGAAG 
ACAGTTCTGA AGCGGGTCCT GAAGGAGCTC TGGCGGGTGG TGATGAACAC CATGGAGCGG 
GTTATTGTCC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT AACTGCTGCC 
AAGGAGCTGA GCCAGCTTTC CAAACTCAAG GACCACATGG TACGGGAGGA AACACGGAAT 
CTCACTCCAA AGCAGTGTGC TGTCCTCGAC TTGGCCTTGG ACACCATCAA GCAATACTTC 
CATGCAGGAG GCAATGGTCT GAAGAAAACC TTCCTGGAAA AGAGCCCAGA CCTGCAGTCT 
CTGCGTTATG CCCTCTCTCT GTATACACAG ACTACAGACA CTCTCATCAA GACATTTGTG 
CGCTCACAGA CTGCCCAGGT ACATGATGGG AAAGGTATTA GGTTTACTGC TAACGAGGAC 
ATTCGGCCAG AAAAGGGGGC TGGTGTGGAT GACCCCGTGG GAGAAGTCTC TATTCAGGTG 
GACTTATTTA CACATCCTGG CACTGGGGAG CATAAGGTCA CAGTGAAAGT GGTAGCAGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCTT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGATAA GAAGAGGAAG TTCACAACCA AGTCTAAAAG TAACAACTGG 
ACTCCCAAGT ACAACGAGAC GTTTCACTTC CTCCTGGGAA ATGAAGAGGG TCCAGAGGCC 
TATGAATTGC AGATCTGTGT GAAGGATTAC TGCTTTGCCC GGGAGGATCG TGTGATAGGA 
CTGGCAGTGA TGCCCCTGAG GGATGTGGCA GCTAAGGGCA GCTGTGCCTG CTGGTGCCCA 
CTGGGCCGGA AGATCCATAT GGATGAGACA GGCATGACCA TTCTCCGGAT TCTGTCTCAG 
AGGAGCAATG ACGAGGTAGC CCGAGAGTTC GTGAAGCTCA AATCAGAGTC TCGATCCACA 
GAGGAAGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537786.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537849.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant X1, mRNA.

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CloneID	OMu32852
Clone ID Related Accession (Same CDS sequence)	XM_017320115.1 , XM_017320115.2 , XM_006537786.3 , XM_006537786.4
Accession Version	XM_006537786.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537786.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGCAGGAACA ACCTCTAGAG GAACCAGGGC CCAGCATTCG GAACTTGGAT 
TTCTGGCCCA AACTTATCAC ACTCATTGTT TCGATCATAG AGGAGGATAA GAATTCCTAC 
ACACCTGTTC TGAGCCAGTT TCCTCAGGAG TTGAACGTAG GAAAAGTCAG TGCAGAAGTG 
ATGTGGCATC TGTTTGCTCA AGACATGAAA TATGCACTGG AGGAACACGA GAAAGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TATGTGCGCG ATCTGCCTGC CCTCCAGGGG CAAGTGCCTG AGTACCCAGC GTGGTTTGAG 
CAGTTTGTGC TACAGTGGCT AGATGAGAAT GAAGATGTGT CACTGGAATT CCTTCGTGGG 
GCCTTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC CTTGTTTTCC 
TGTTCTGTGG TGGATGTCTT TACACAACTC AACCAGAGCT TTGAGATCAT CCGGAAGTTG 
GAATGTCCAG ACCCCAACAT CCTTGCCCAC TACATGAGAA GGTTTGCTAA GACCATTGGG 
AAAGTGCTGA TGCAGTATGC AGACATCTTA TCAAAGAACT TCCCAGCTTA CTGCACAAAG 
GAGAGACTGC CCTGTATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAAGAGCTG GACTCCGAAG CTGCAGACAG TCTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTTAGCA TGGTGTTTGG AAACAGCTTC 
CAGGTGCGGA TTGATGAATG TGTTCGACAA ATGGCTGACA TCCTGGGCCA AGTTCGGGGC 
ACAGGGAATG CATCCCCTAA CGCCAGGGCC TCGGTGGCTC AGGATGCAGA TAGTGTGCTG 
CGACCTCTCA TGGACTTCCT GGATGGCAAC CTCACGCTGT TTGCCACTGT GTGTGAGAAG 
ACAGTTCTGA AGCGGGTCCT GAAGGAGCTC TGGCGGGTGG TGATGAACAC CATGGAGCGG 
GTTATTGTCC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT AACTGCTGCC 
AAGGAGCTGA GCCAGCTTTC CAAACTCAAG GACCACATGG TACGGGAGGA AACACGGAAT 
CTCACTCCAA AGCAGTGTGC TGTCCTCGAC TTGGCCTTGG ACACCATCAA GCAATACTTC 
CATGCAGGAG GCAATGGTCT GAAGAAAACC TTCCTGGAAA AGAGCCCAGA CCTGCAGTCT 
CTGCGTTATG CCCTCTCTCT GTATACACAG ACTACAGACA CTCTCATCAA GACATTTGTG 
CGCTCACAGA CTGCCCAGGT ACATGATGGG AAAGGTATTA GGTTTACTGC TAACGAGGAC 
ATTCGGCCAG AAAAGGGGGC TGGTGTGGAT GACCCCGTGG GAGAAGTCTC TATTCAGGTG 
GACTTATTTA CACATCCTGG CACTGGGGAG CATAAGGTCA CAGTGAAAGT GGTAGCAGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCTT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGATAA GAAGAGGAAG TTCACAACCA AGTCTAAAAG TAACAACTGG 
ACTCCCAAGT ACAACGAGAC GTTTCACTTC CTCCTGGGAA ATGAAGAGGG TCCAGAGGCC 
TATGAATTGC AGATCTGTGT GAAGGATTAC TGCTTTGCCC GGGAGGATCG TGTGATAGGA 
CTGGCAGTGA TGCCCCTGAG GGATGTGGCA GCTAAGGGCA GCTGTGCCTG CTGGTGCCCA 
CTGGGCCGGA AGATCCATAT GGATGAGACA GGCATGACCA TTCTCCGGAT TCTGTCTCAG 
AGGAGCAATG ACGAGGTAGC CCGAGAGTTC GTGAAGCTCA AATCAGAGTC TCGATCCACA 
GAGGAAGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537849.1
CDS	2563..8517
Translation	MKRLLRESEEEIMLTLGPSSSLSPDKVRAETVCSVKSKSTGPTGSLPEDNFPLPCCDSTTSGERDGDLPQLCSFEQQANNQPSLASTACASGSDSRELSPASITNCSDPSERNQARPILPRGPGQRCRYEQQEPLGDVVEYIIRELQGISRLQSEIAELQQHLNQVQGSVDEVSSCVDSVLSEIEGLHVGSSSLGKVCSGEKAQELHVERSREEAILYLYGLPEHDGENTMELVDNFLAKHLCVNGMQCNRYIQEAYRTGTAPAPRPTVVKLVHLEHRDLILQKSILLQSVGVRVATREEPVWSEGCEDPPKESLSCLQQLQDHSWNSLNQDKPALRLETGNRKQMTEPHQIRTQNQQRGPQASEHQGPRLLPKDGLAKQSDVSKLQEEVKGTSGASWVTSDCCGELSLLHPLEGSSSVLISKEEDCEKLQIFKQGRQEHEACKVTKLQSDCSNSIKDSSCLSLSGPLKADMVNTEDKMLGCEAGLDILSPKQLEDLLADKSRRFATLSPDSTMEEVIIGPETLSNMVHIDLNEEENCTAQVLKDVFDKSSRVLADSQEDEDVEIKFHTTKLSRAIQHFRLALQGVFQKLENNGSISPEDLESNESGSQSENSDRLLWTVSSGGAHDCSVESPASQGSESLLSVVSGGVGISIQGDQTSQAPSNFSLASNNSPFANSLLSFPLAPCLGSETCSRPDSPNQGKLSLEQVCAETIYLNKCINNFKNVLREKRLRQKKLLQELVQTASHLSVEDIPSEGNQEAIQISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537786.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537786.4

ORF Insert Sequence:

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGCAGGAACA ACCTCTAGAG GAACCAGGGC CCAGCATTCG GAACTTGGAT 
TTCTGGCCCA AACTTATCAC ACTCATTGTT TCGATCATAG AGGAGGATAA GAATTCCTAC 
ACACCTGTTC TGAGCCAGTT TCCTCAGGAG TTGAACGTAG GAAAAGTCAG TGCAGAAGTG 
ATGTGGCATC TGTTTGCTCA AGACATGAAA TATGCACTGG AGGAACACGA GAAAGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TATGTGCGCG ATCTGCCTGC CCTCCAGGGG CAAGTGCCTG AGTACCCAGC GTGGTTTGAG 
CAGTTTGTGC TACAGTGGCT AGATGAGAAT GAAGATGTGT CACTGGAATT CCTTCGTGGG 
GCCTTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC CTTGTTTTCC 
TGTTCTGTGG TGGATGTCTT TACACAACTC AACCAGAGCT TTGAGATCAT CCGGAAGTTG 
GAATGTCCAG ACCCCAACAT CCTTGCCCAC TACATGAGAA GGTTTGCTAA GACCATTGGG 
AAAGTGCTGA TGCAGTATGC AGACATCTTA TCAAAGAACT TCCCAGCTTA CTGCACAAAG 
GAGAGACTGC CCTGTATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAAGAGCTG GACTCCGAAG CTGCAGACAG TCTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTTAGCA TGGTGTTTGG AAACAGCTTC 
CAGGTGCGGA TTGATGAATG TGTTCGACAA ATGGCTGACA TCCTGGGCCA AGTTCGGGGC 
ACAGGGAATG CATCCCCTAA CGCCAGGGCC TCGGTGGCTC AGGATGCAGA TAGTGTGCTG 
CGACCTCTCA TGGACTTCCT GGATGGCAAC CTCACGCTGT TTGCCACTGT GTGTGAGAAG 
ACAGTTCTGA AGCGGGTCCT GAAGGAGCTC TGGCGGGTGG TGATGAACAC CATGGAGCGG 
GTTATTGTCC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT AACTGCTGCC 
AAGGAGCTGA GCCAGCTTTC CAAACTCAAG GACCACATGG TACGGGAGGA AACACGGAAT 
CTCACTCCAA AGCAGTGTGC TGTCCTCGAC TTGGCCTTGG ACACCATCAA GCAATACTTC 
CATGCAGGAG GCAATGGTCT GAAGAAAACC TTCCTGGAAA AGAGCCCAGA CCTGCAGTCT 
CTGCGTTATG CCCTCTCTCT GTATACACAG ACTACAGACA CTCTCATCAA GACATTTGTG 
CGCTCACAGA CTGCCCAGGT ACATGATGGG AAAGGTATTA GGTTTACTGC TAACGAGGAC 
ATTCGGCCAG AAAAGGGGGC TGGTGTGGAT GACCCCGTGG GAGAAGTCTC TATTCAGGTG 
GACTTATTTA CACATCCTGG CACTGGGGAG CATAAGGTCA CAGTGAAAGT GGTAGCAGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCTT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGATAA GAAGAGGAAG TTCACAACCA AGTCTAAAAG TAACAACTGG 
ACTCCCAAGT ACAACGAGAC GTTTCACTTC CTCCTGGGAA ATGAAGAGGG TCCAGAGGCC 
TATGAATTGC AGATCTGTGT GAAGGATTAC TGCTTTGCCC GGGAGGATCG TGTGATAGGA 
CTGGCAGTGA TGCCCCTGAG GGATGTGGCA GCTAAGGGCA GCTGTGCCTG CTGGTGCCCA 
CTGGGCCGGA AGATCCATAT GGATGAGACA GGCATGACCA TTCTCCGGAT TCTGTCTCAG 
AGGAGCAATG ACGAGGTAGC CCGAGAGTTC GTGAAGCTCA AATCAGAGTC TCGATCCACA 
GAGGAAGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537788.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537851.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant X3, mRNA.

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CloneID	OMu32853
Clone ID Related Accession (Same CDS sequence)	XM_006537788.3 , XM_006537788.4
Accession Version	XM_006537788.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537788.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGGAACAACC TCTAGAGGAA CCAGGGCCCA GCATTCGGAA CTTGGATTTC 
TGGCCCAAAC TTATCACACT CATTGTTTCG ATCATAGAGG AGGATAAGAA TTCCTACACA 
CCTGTTCTGA GCCAGTTTCC TCAGGAGTTG AACGTAGGAA AAGTCAGTGC AGAAGTGATG 
TGGCATCTGT TTGCTCAAGA CATGAAATAT GCACTGGAGG AACACGAGAA AGACCGGCTG 
TGTAAGAGTG CTGACTACAT GAACCTGCAC TTCAAGGTGA AGTGGCTCCA CAATGAATAT 
GTGCGCGATC TGCCTGCCCT CCAGGGGCAA GTGCCTGAGT ACCCAGCGTG GTTTGAGCAG 
TTTGTGCTAC AGTGGCTAGA TGAGAATGAA GATGTGTCAC TGGAATTCCT TCGTGGGGCC 
TTGGAACGAG ATAAGAAGGA TGGGTTCCAG CAGACATCAG AGCATGCCTT GTTTTCCTGT 
TCTGTGGTGG ATGTCTTTAC ACAACTCAAC CAGAGCTTTG AGATCATCCG GAAGTTGGAA 
TGTCCAGACC CCAACATCCT TGCCCACTAC ATGAGAAGGT TTGCTAAGAC CATTGGGAAA 
GTGCTGATGC AGTATGCAGA CATCTTATCA AAGAACTTCC CAGCTTACTG CACAAAGGAG 
AGACTGCCCT GTATCCTGAT GAACAACATG CAGCAACTGA GGGTCCAGCT GGAGAAAATG 
TTTGAGGCCA TGGGAGGCAA AGAGCTGGAC TCCGAAGCTG CAGACAGTCT GAAGGAGCTG 
CAGGTGAAAC TGAATACAGT TCTGGATGAG CTTAGCATGG TGTTTGGAAA CAGCTTCCAG 
GTGCGGATTG ATGAATGTGT TCGACAAATG GCTGACATCC TGGGCCAAGT TCGGGGCACA 
GGGAATGCAT CCCCTAACGC CAGGGCCTCG GTGGCTCAGG ATGCAGATAG TGTGCTGCGA 
CCTCTCATGG ACTTCCTGGA TGGCAACCTC ACGCTGTTTG CCACTGTGTG TGAGAAGACA 
GTTCTGAAGC GGGTCCTGAA GGAGCTCTGG CGGGTGGTGA TGAACACCAT GGAGCGGGTT 
ATTGTCCTGC CCCCACTCAC TGACCAGACG GGCACCCAGC TGATCTTAAC TGCTGCCAAG 
GAGCTGAGCC AGCTTTCCAA ACTCAAGGAC CACATGGTAC GGGAGGAAAC ACGGAATCTC 
ACTCCAAAGC AGTGTGCTGT CCTCGACTTG GCCTTGGACA CCATCAAGCA ATACTTCCAT 
GCAGGAGGCA ATGGTCTGAA GAAAACCTTC CTGGAAAAGA GCCCAGACCT GCAGTCTCTG 
CGTTATGCCC TCTCTCTGTA TACACAGACT ACAGACACTC TCATCAAGAC ATTTGTGCGC 
TCACAGACTG CCCAGGTACA TGATGGGAAA GGTATTAGGT TTACTGCTAA CGAGGACATT 
CGGCCAGAAA AGGGGGCTGG TGTGGATGAC CCCGTGGGAG AAGTCTCTAT TCAGGTGGAC 
TTATTTACAC ATCCTGGCAC TGGGGAGCAT AAGGTCACAG TGAAAGTGGT AGCAGCCAAT 
GACCTCAAGT GGCAGACAGC GGGTATGTTC CGGCCTTTTG TGGAAGTGAC CATGGTTGGC 
CCACACCAAA GTGATAAGAA GAGGAAGTTC ACAACCAAGT CTAAAAGTAA CAACTGGACT 
CCCAAGTACA ACGAGACGTT TCACTTCCTC CTGGGAAATG AAGAGGGTCC AGAGGCCTAT 
GAATTGCAGA TCTGTGTGAA GGATTACTGC TTTGCCCGGG AGGATCGTGT GATAGGACTG 
GCAGTGATGC CCCTGAGGGA TGTGGCAGCT AAGGGCAGCT GTGCCTGCTG GTGCCCACTG 
GGCCGGAAGA TCCATATGGA TGAGACAGGC ATGACCATTC TCCGGATTCT GTCTCAGAGG 
AGCAATGACG AGGTAGCCCG AGAGTTCGTG AAGCTCAAAT CAGAGTCTCG ATCCACAGAG 
GAAGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537851.1
CDS	2563..8514
Translation	MKRLLRESEEEIMLTLGPSSSLSPDKVRAETVCSVKSKSTGPTGSLPEDNFPLPCCDSTTSGERDGDLPQLCSFEQQANNQPSLASTACASGSDSRELSPASITNCSDPSERNQARPILPRGPGQRCRYEQQEPLGDVVEYIIRELQGISRLQSEIAELQQHLNQVQGSVDEVSSCVDSVLSEIEGLHVGSSSLGKVCSGEKAQELHVERSREEAILYLYGLPEHDGENTMELVDNFLAKHLCVNGMQCNRYIQEAYRTGTAPAPRPTVVKLVHLEHRDLILQKSILLQSVGVRVATREEPVWSEGCEDPPKESLSCLQQLQDHSWNSLNQDKPALRLETGNRKQMTEPHQIRTQNQQRGPQASEHQGPRLLPKDGLAKQSDVSKLQEEVKGTSGASWVTSDCCGELSLLHPLEGSSSVLISKEEDCEKLQIFKQGRQEHEACKVTKLQSDCSNSIKDSSCLSLSGPLKADMVNTEDKMLGCEAGLDILSPKQLEDLLADKSRRFATLSPDSTMEEVIIGPETLSNMVHIDLNEEENCTAQVLKDVFDKSSRVLADSQEDEDVEIKFHTTKLSRAIQHFRLALQGVFQKLENNGSISPEDLESNESGSQSENSDRLLWTVSSGGAHDCSVESPASQGSESLLSVVSGGVGISIQGDQTSQAPSNFSLASNNSPFANSLLSFPLAPCLGSETCSRPDSPNQGKLSLEQVCAETIYLNKCINNFKNVLREKRLRQKKLLQELVQTASHLSVEDIPSEGNQEAIQISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537788.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537788.4

ORF Insert Sequence:

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGGAACAACC TCTAGAGGAA CCAGGGCCCA GCATTCGGAA CTTGGATTTC 
TGGCCCAAAC TTATCACACT CATTGTTTCG ATCATAGAGG AGGATAAGAA TTCCTACACA 
CCTGTTCTGA GCCAGTTTCC TCAGGAGTTG AACGTAGGAA AAGTCAGTGC AGAAGTGATG 
TGGCATCTGT TTGCTCAAGA CATGAAATAT GCACTGGAGG AACACGAGAA AGACCGGCTG 
TGTAAGAGTG CTGACTACAT GAACCTGCAC TTCAAGGTGA AGTGGCTCCA CAATGAATAT 
GTGCGCGATC TGCCTGCCCT CCAGGGGCAA GTGCCTGAGT ACCCAGCGTG GTTTGAGCAG 
TTTGTGCTAC AGTGGCTAGA TGAGAATGAA GATGTGTCAC TGGAATTCCT TCGTGGGGCC 
TTGGAACGAG ATAAGAAGGA TGGGTTCCAG CAGACATCAG AGCATGCCTT GTTTTCCTGT 
TCTGTGGTGG ATGTCTTTAC ACAACTCAAC CAGAGCTTTG AGATCATCCG GAAGTTGGAA 
TGTCCAGACC CCAACATCCT TGCCCACTAC ATGAGAAGGT TTGCTAAGAC CATTGGGAAA 
GTGCTGATGC AGTATGCAGA CATCTTATCA AAGAACTTCC CAGCTTACTG CACAAAGGAG 
AGACTGCCCT GTATCCTGAT GAACAACATG CAGCAACTGA GGGTCCAGCT GGAGAAAATG 
TTTGAGGCCA TGGGAGGCAA AGAGCTGGAC TCCGAAGCTG CAGACAGTCT GAAGGAGCTG 
CAGGTGAAAC TGAATACAGT TCTGGATGAG CTTAGCATGG TGTTTGGAAA CAGCTTCCAG 
GTGCGGATTG ATGAATGTGT TCGACAAATG GCTGACATCC TGGGCCAAGT TCGGGGCACA 
GGGAATGCAT CCCCTAACGC CAGGGCCTCG GTGGCTCAGG ATGCAGATAG TGTGCTGCGA 
CCTCTCATGG ACTTCCTGGA TGGCAACCTC ACGCTGTTTG CCACTGTGTG TGAGAAGACA 
GTTCTGAAGC GGGTCCTGAA GGAGCTCTGG CGGGTGGTGA TGAACACCAT GGAGCGGGTT 
ATTGTCCTGC CCCCACTCAC TGACCAGACG GGCACCCAGC TGATCTTAAC TGCTGCCAAG 
GAGCTGAGCC AGCTTTCCAA ACTCAAGGAC CACATGGTAC GGGAGGAAAC ACGGAATCTC 
ACTCCAAAGC AGTGTGCTGT CCTCGACTTG GCCTTGGACA CCATCAAGCA ATACTTCCAT 
GCAGGAGGCA ATGGTCTGAA GAAAACCTTC CTGGAAAAGA GCCCAGACCT GCAGTCTCTG 
CGTTATGCCC TCTCTCTGTA TACACAGACT ACAGACACTC TCATCAAGAC ATTTGTGCGC 
TCACAGACTG CCCAGGTACA TGATGGGAAA GGTATTAGGT TTACTGCTAA CGAGGACATT 
CGGCCAGAAA AGGGGGCTGG TGTGGATGAC CCCGTGGGAG AAGTCTCTAT TCAGGTGGAC 
TTATTTACAC ATCCTGGCAC TGGGGAGCAT AAGGTCACAG TGAAAGTGGT AGCAGCCAAT 
GACCTCAAGT GGCAGACAGC GGGTATGTTC CGGCCTTTTG TGGAAGTGAC CATGGTTGGC 
CCACACCAAA GTGATAAGAA GAGGAAGTTC ACAACCAAGT CTAAAAGTAA CAACTGGACT 
CCCAAGTACA ACGAGACGTT TCACTTCCTC CTGGGAAATG AAGAGGGTCC AGAGGCCTAT 
GAATTGCAGA TCTGTGTGAA GGATTACTGC TTTGCCCGGG AGGATCGTGT GATAGGACTG 
GCAGTGATGC CCCTGAGGGA TGTGGCAGCT AAGGGCAGCT GTGCCTGCTG GTGCCCACTG 
GGCCGGAAGA TCCATATGGA TGAGACAGGC ATGACCATTC TCCGGATTCT GTCTCAGAGG 
AGCAATGACG AGGTAGCCCG AGAGTTCGTG AAGCTCAAAT CAGAGTCTCG ATCCACAGAG 
GAAGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537789.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537852.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant X4, mRNA.

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CloneID	OMu32854
Clone ID Related Accession (Same CDS sequence)	XM_006537789.3 , XM_006537789.4
Accession Version	XM_006537789.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537789.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGTCACTGGT CCAGTCACGG AAAGCTGGGA TTACATCTGC TATGGCTACA 
CGTACCTCTC TCAAGGATGA AGAACTGAAA TCTCATGTGT ATAAGAAAAC TCTGCAGGCC 
TTAATCTACC CCATCTCGTG TACCACGCCC CACAACTTTG AGGTCTGGTC TGCCACTACA 
CCTACCTATT GCTATGAGTG TGAAGGCTTG CTCTGGGGCC TTGCCCGGCA AGGCATGCGC 
TGCAGCGAGT GTGGAGTCAA GTGCCATGAG AAGTGCCAGG ACCTGCTCAA TGCTGACTGC 
CTACAGCGGG CTGCTGAAAA GAGCTCTAAG CATGGAGCTG AGGACCGAAC TCAGAACATT 
ATCATGGCCA TGAAGGACCG TATGAAGATC CGAGAACGGA ATAAACCAGA GATCTTTGAG 
GTTATCCGGG ATGTGTTCAC AGTGAGCAAA GTTGCGCATG TGCAGCAGAT GAAAACAGTA 
AAGCAGAGTG TCCTGGATGG CACCTCCAAG TGGTCAGCCA AAATTACCAT CACGGTGGTT 
TGTGCCCAGG GCCTACAAGC CAAAGACAAG ACAGGATCCA GTGACCCTTA TGTGACTGTG 
CAAGTTGGGA AAACCAAGAA GCGCACCAAG ACCATTTTTG GAAATTTGAA TCCTGTTTGG 
GAAGAGAAGT TCCATTTTGA GTGCCACAAC TCTTCTGATC GAATTAAGGT ACGCGTGTGG 
GATGAGGATG ATGACATCAA GTCCAGAGTA AAGCAGCGGC TAAAGCGAGA GTCAGATGAT 
TTCCTTGGCC AAACTATCAT TGAAGTTCGA ACGCTGAGTG GAGAGATGGA TGTCTGGTAC 
AATCTGGAGA AGAGAACAGA CAAGTCAGCT GTCTCAGGGG CTATCCGACT TCAAATCAGT 
GTGGAGATCA AGGGGGAGGA GAAGGTAGCC CCATACCATG TACAATATAC ATGTCTCCAT 
GAGAACCTTT TCCATTACCT CACAGACATT CAGGGCAGTG GAGGAGTCTG GATCCCAGAG 
GCTCGGGGGG ACGATGCATG GAAGGTGTAC TTTGATGAGA CAGCCCAAGA AATTGTGGAT 
GAATTCGCTA TGCGCTATGG CATCGAGTCC ATTTATCAGG CCATGACGCA CTTTGCATGT 
TTGTCATCCA AGTACATGTG TCCTGGTGTA CCAGCCGTGA TGAGCACCTT ACTGGCCAAC 
ATCAATGCCT ATTATGCTCA CACAACTGCC TCCACTAACG TCTCTGCATC TGATCGTTTT 
GCAGCCTCCA ACTTTGGTAA AGAAAGATTT GTAAAACTGC TGGACCAGCT ACACAACTCA 
CTGAGGATAG ACCTCTCTAC ATACAGGAAT AACTTTCCTG CTGGGAGCCC TGAGCGGCTT 
CAGGATTTAA AGTCTACAGT GGATTTGCTG ACCAGCATTA CTTTCTTCAG GATGAAGGTG 
CAAGAACTTC AGAGCCCTCC AAGAGCCAGC CAGGTGGTAA AGGACTGTGT GAAAGCCTGC 
TTGAACTCTA CATATGAATA CATCTTCAAC AACTGCCATG ACCTCTACAG TCACCAGTAT 
CAGCTGCAGG AACAACCTCT AGAGGAACCA GGGCCCAGCA TTCGGAACTT GGATTTCTGG 
CCCAAACTTA TCACACTCAT TGTTTCGATC ATAGAGGAGG ATAAGAATTC CTACACACCT 
GTTCTGAGCC AGTTTCCTCA GGAGTTGAAC GTAGGAAAAG TCAGTGCAGA AGTGATGTGG 
CATCTGTTTG CTCAAGACAT GAAATATGCA CTGGAGGAAC ACGAGAAAGA CCGGCTGTGT 
AAGAGTGCTG ACTACATGAA CCTGCACTTC AAGGTGAAGT GGCTCCACAA TGAATATGTG 
CGCGATCTGC CTGCCCTCCA GGGGCAAGTG CCTGAGTACC CAGCGTGGTT TGAGCAGTTT 
GTGCTACAGT GGCTAGATGA GAATGAAGAT GTGTCACTGG AATTCCTTCG TGGGGCCTTG 
GAACGAGATA AGAAGGATGG GTTCCAGCAG ACATCAGAGC ATGCCTTGTT TTCCTGTTCT 
GTGGTGGATG TCTTTACACA ACTCAACCAG AGCTTTGAGA TCATCCGGAA GTTGGAATGT 
CCAGACCCCA ACATCCTTGC CCACTACATG AGAAGGTTTG CTAAGACCAT TGGGAAAGTG 
CTGATGCAGT ATGCAGACAT CTTATCAAAG AACTTCCCAG CTTACTGCAC AAAGGAGAGA 
CTGCCCTGTA TCCTGATGAA CAACATGCAG CAACTGAGGG TCCAGCTGGA GAAAATGTTT 
GAGGCCATGG GAGGCAAAGA GCTGGACTCC GAAGCTGCAG ACAGTCTGAA GGAGCTGCAG 
GTGAAACTGA ATACAGTTCT GGATGAGCTT AGCATGGTGT TTGGAAACAG CTTCCAGGTG 
CGGATTGATG AATGTGTTCG ACAAATGGCT GACATCCTGG GCCAAGTTCG GGGCACAGGG 
AATGCATCCC CTAACGCCAG GGCCTCGGTG GCTCAGGATG CAGATAGTGT GCTGCGACCT 
CTCATGGACT TCCTGGATGG CAACCTCACG CTGTTTGCCA CTGTGTGTGA GAAGACAGTT 
CTGAAGCGGG TCCTGAAGGA GCTCTGGCGG GTGGTGATGA ACACCATGGA GCGGGTTATT 
GTCCTGCCCC CACTCACTGA CCAGACGGGC ACCCAGCTGA TCTTAACTGC TGCCAAGGAG 
CTGAGCCAGC TTTCCAAACT CAAGGACCAC ATGGTACGGG AGGAAACACG GAATCTCACT 
CCAAAGCAGT GTGCTGTCCT CGACTTGGCC TTGGACACCA TCAAGCAATA CTTCCATGCA 
GGAGGCAATG GTCTGAAGAA AACCTTCCTG GAAAAGAGCC CAGACCTGCA GTCTCTGCGT 
TATGCCCTCT CTCTGTATAC ACAGACTACA GACACTCTCA TCAAGACATT TGTGCGCTCA 
CAGACTGCCC AGGTACATGA TGGGAAAGGT ATTAGGTTTA CTGCTAACGA GGACATTCGG 
CCAGAAAAGG GGGCTGGTGT GGATGACCCC GTGGGAGAAG TCTCTATTCA GGTGGACTTA 
TTTACACATC CTGGCACTGG GGAGCATAAG GTCACAGTGA AAGTGGTAGC AGCCAATGAC 
CTCAAGTGGC AGACAGCGGG TATGTTCCGG CCTTTTGTGG AAGTGACCAT GGTTGGCCCA 
CACCAAAGTG ATAAGAAGAG GAAGTTCACA ACCAAGTCTA AAAGTAACAA CTGGACTCCC 
AAGTACAACG AGACGTTTCA CTTCCTCCTG GGAAATGAAG AGGGTCCAGA GGCCTATGAA 
TTGCAGATCT GTGTGAAGGA TTACTGCTTT GCCCGGGAGG ATCGTGTGAT AGGACTGGCA 
GTGATGCCCC TGAGGGATGT GGCAGCTAAG GGCAGCTGTG CCTGCTGGTG CCCACTGGGC 
CGGAAGATCC ATATGGATGA GACAGGCATG ACCATTCTCC GGATTCTGTC TCAGAGGAGC 
AATGACGAGG TAGCCCGAGA GTTCGTGAAG CTCAAATCAG AGTCTCGATC CACAGAGGAA 
GGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537852.1
CDS	2563..8511
Translation	MKRLLRESEEEIMLTLGPSSSLSPDKVRAETVCSVKSKSTGPTGSLPEDNFPLPCCDSTTSGERDGDLPQLCSFEQQANNQPSLASTACASGSDSRELSPASITNCSDPSERNQARPILPRGPGQRCRYEQQEPLGDVVEYIIRELQGISRLQSEIAELQQHLNQVQGSVDEVSSCVDSVLSEIEGLHVGSSSLGKVCSGEKAQELHVERSREEAILYLYGLPEHDGENTMELVDNFLAKHLCVNGMQCNRYIQEAYRTGTAPAPRPTVVKLVHLEHRDLILQKSILLQSVGVRVATREEPVWSEGCEDPPKESLSCLQQLQDHSWNSLNQDKPALRLETGNRKQMTEPHQIRTQNQQRGPQASEHQGPRLLPKDGLAKQSDVSKLQEEVKGTSGASWVTSDCCGELSLLHPLEGSSSVLISKEEDCEKLQIFKQGRQEHEACKVTKLQSDCSNSIKDSSCLSLSGPLKADMVNTEDKMLGCEAGLDILSPKQLEDLLADKSRRFATLSPDSTMEEVIIGPETLSNMVHIDLNEEENCTAQVLKDVFDKSSRVLADSQEDEDVEIKFHTTKLSRAIQHFRLALQGVFQKLENNGSISPEDLESNESGSQSENSDRLLWTVSSGGAHDCSVESPASQGSESLLSVVSGGVGISIQGDQTSQAPSNFSLASNNSPFANSLLSFPLAPCLGSETCSRPDSPNQGKLSLEQVCAETIYLNKCINNFKNVLREKRLRQKKLLQELVQTASHLSVEDIPSEGNQEAIQISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537789.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537789.4

ORF Insert Sequence:

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGTCACTGGT CCAGTCACGG AAAGCTGGGA TTACATCTGC TATGGCTACA 
CGTACCTCTC TCAAGGATGA AGAACTGAAA TCTCATGTGT ATAAGAAAAC TCTGCAGGCC 
TTAATCTACC CCATCTCGTG TACCACGCCC CACAACTTTG AGGTCTGGTC TGCCACTACA 
CCTACCTATT GCTATGAGTG TGAAGGCTTG CTCTGGGGCC TTGCCCGGCA AGGCATGCGC 
TGCAGCGAGT GTGGAGTCAA GTGCCATGAG AAGTGCCAGG ACCTGCTCAA TGCTGACTGC 
CTACAGCGGG CTGCTGAAAA GAGCTCTAAG CATGGAGCTG AGGACCGAAC TCAGAACATT 
ATCATGGCCA TGAAGGACCG TATGAAGATC CGAGAACGGA ATAAACCAGA GATCTTTGAG 
GTTATCCGGG ATGTGTTCAC AGTGAGCAAA GTTGCGCATG TGCAGCAGAT GAAAACAGTA 
AAGCAGAGTG TCCTGGATGG CACCTCCAAG TGGTCAGCCA AAATTACCAT CACGGTGGTT 
TGTGCCCAGG GCCTACAAGC CAAAGACAAG ACAGGATCCA GTGACCCTTA TGTGACTGTG 
CAAGTTGGGA AAACCAAGAA GCGCACCAAG ACCATTTTTG GAAATTTGAA TCCTGTTTGG 
GAAGAGAAGT TCCATTTTGA GTGCCACAAC TCTTCTGATC GAATTAAGGT ACGCGTGTGG 
GATGAGGATG ATGACATCAA GTCCAGAGTA AAGCAGCGGC TAAAGCGAGA GTCAGATGAT 
TTCCTTGGCC AAACTATCAT TGAAGTTCGA ACGCTGAGTG GAGAGATGGA TGTCTGGTAC 
AATCTGGAGA AGAGAACAGA CAAGTCAGCT GTCTCAGGGG CTATCCGACT TCAAATCAGT 
GTGGAGATCA AGGGGGAGGA GAAGGTAGCC CCATACCATG TACAATATAC ATGTCTCCAT 
GAGAACCTTT TCCATTACCT CACAGACATT CAGGGCAGTG GAGGAGTCTG GATCCCAGAG 
GCTCGGGGGG ACGATGCATG GAAGGTGTAC TTTGATGAGA CAGCCCAAGA AATTGTGGAT 
GAATTCGCTA TGCGCTATGG CATCGAGTCC ATTTATCAGG CCATGACGCA CTTTGCATGT 
TTGTCATCCA AGTACATGTG TCCTGGTGTA CCAGCCGTGA TGAGCACCTT ACTGGCCAAC 
ATCAATGCCT ATTATGCTCA CACAACTGCC TCCACTAACG TCTCTGCATC TGATCGTTTT 
GCAGCCTCCA ACTTTGGTAA AGAAAGATTT GTAAAACTGC TGGACCAGCT ACACAACTCA 
CTGAGGATAG ACCTCTCTAC ATACAGGAAT AACTTTCCTG CTGGGAGCCC TGAGCGGCTT 
CAGGATTTAA AGTCTACAGT GGATTTGCTG ACCAGCATTA CTTTCTTCAG GATGAAGGTG 
CAAGAACTTC AGAGCCCTCC AAGAGCCAGC CAGGTGGTAA AGGACTGTGT GAAAGCCTGC 
TTGAACTCTA CATATGAATA CATCTTCAAC AACTGCCATG ACCTCTACAG TCACCAGTAT 
CAGCTGCAGG AACAACCTCT AGAGGAACCA GGGCCCAGCA TTCGGAACTT GGATTTCTGG 
CCCAAACTTA TCACACTCAT TGTTTCGATC ATAGAGGAGG ATAAGAATTC CTACACACCT 
GTTCTGAGCC AGTTTCCTCA GGAGTTGAAC GTAGGAAAAG TCAGTGCAGA AGTGATGTGG 
CATCTGTTTG CTCAAGACAT GAAATATGCA CTGGAGGAAC ACGAGAAAGA CCGGCTGTGT 
AAGAGTGCTG ACTACATGAA CCTGCACTTC AAGGTGAAGT GGCTCCACAA TGAATATGTG 
CGCGATCTGC CTGCCCTCCA GGGGCAAGTG CCTGAGTACC CAGCGTGGTT TGAGCAGTTT 
GTGCTACAGT GGCTAGATGA GAATGAAGAT GTGTCACTGG AATTCCTTCG TGGGGCCTTG 
GAACGAGATA AGAAGGATGG GTTCCAGCAG ACATCAGAGC ATGCCTTGTT TTCCTGTTCT 
GTGGTGGATG TCTTTACACA ACTCAACCAG AGCTTTGAGA TCATCCGGAA GTTGGAATGT 
CCAGACCCCA ACATCCTTGC CCACTACATG AGAAGGTTTG CTAAGACCAT TGGGAAAGTG 
CTGATGCAGT ATGCAGACAT CTTATCAAAG AACTTCCCAG CTTACTGCAC AAAGGAGAGA 
CTGCCCTGTA TCCTGATGAA CAACATGCAG CAACTGAGGG TCCAGCTGGA GAAAATGTTT 
GAGGCCATGG GAGGCAAAGA GCTGGACTCC GAAGCTGCAG ACAGTCTGAA GGAGCTGCAG 
GTGAAACTGA ATACAGTTCT GGATGAGCTT AGCATGGTGT TTGGAAACAG CTTCCAGGTG 
CGGATTGATG AATGTGTTCG ACAAATGGCT GACATCCTGG GCCAAGTTCG GGGCACAGGG 
AATGCATCCC CTAACGCCAG GGCCTCGGTG GCTCAGGATG CAGATAGTGT GCTGCGACCT 
CTCATGGACT TCCTGGATGG CAACCTCACG CTGTTTGCCA CTGTGTGTGA GAAGACAGTT 
CTGAAGCGGG TCCTGAAGGA GCTCTGGCGG GTGGTGATGA ACACCATGGA GCGGGTTATT 
GTCCTGCCCC CACTCACTGA CCAGACGGGC ACCCAGCTGA TCTTAACTGC TGCCAAGGAG 
CTGAGCCAGC TTTCCAAACT CAAGGACCAC ATGGTACGGG AGGAAACACG GAATCTCACT 
CCAAAGCAGT GTGCTGTCCT CGACTTGGCC TTGGACACCA TCAAGCAATA CTTCCATGCA 
GGAGGCAATG GTCTGAAGAA AACCTTCCTG GAAAAGAGCC CAGACCTGCA GTCTCTGCGT 
TATGCCCTCT CTCTGTATAC ACAGACTACA GACACTCTCA TCAAGACATT TGTGCGCTCA 
CAGACTGCCC AGGTACATGA TGGGAAAGGT ATTAGGTTTA CTGCTAACGA GGACATTCGG 
CCAGAAAAGG GGGCTGGTGT GGATGACCCC GTGGGAGAAG TCTCTATTCA GGTGGACTTA 
TTTACACATC CTGGCACTGG GGAGCATAAG GTCACAGTGA AAGTGGTAGC AGCCAATGAC 
CTCAAGTGGC AGACAGCGGG TATGTTCCGG CCTTTTGTGG AAGTGACCAT GGTTGGCCCA 
CACCAAAGTG ATAAGAAGAG GAAGTTCACA ACCAAGTCTA AAAGTAACAA CTGGACTCCC 
AAGTACAACG AGACGTTTCA CTTCCTCCTG GGAAATGAAG AGGGTCCAGA GGCCTATGAA 
TTGCAGATCT GTGTGAAGGA TTACTGCTTT GCCCGGGAGG ATCGTGTGAT AGGACTGGCA 
GTGATGCCCC TGAGGGATGT GGCAGCTAAG GGCAGCTGTG CCTGCTGGTG CCCACTGGGC 
CGGAAGATCC ATATGGATGA GACAGGCATG ACCATTCTCC GGATTCTGTC TCAGAGGAGC 
AATGACGAGG TAGCCCGAGA GTTCGTGAAG CTCAAATCAG AGTCTCGATC CACAGAGGAA 
GGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537790.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537853.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant X5, mRNA.

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CloneID	OMu32855
Clone ID Related Accession (Same CDS sequence)	XM_006537790.3 , XM_006537790.4
Accession Version	XM_006537790.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537790.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGCAGGAACA ACCTCTAGAG GAACCAGGGC CCAGCATTCG GAACTTGGAT 
TTCTGGCCCA AACTTATCAC ACTCATTGTT TCGATCATAG AGGAGGATAA GAATTCCTAC 
ACACCTGTTC TGAGCCAGTT TCCTCAGGAG TTGAACGTAG GAAAAGTCAG TGCAGAAGTG 
ATGTGGCATC TGTTTGCTCA AGACATGAAA TATGCACTGG AGGAACACGA GAAAGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TATGTGCGCG ATCTGCCTGC CCTCCAGGGG CAAGTGCCTG AGTACCCAGC GTGGTTTGAG 
CAGTTTGTGC TACAGTGGCT AGATGAGAAT GAAGATGTGT CACTGGAATT CCTTCGTGGG 
GCCTTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC CTTGTTTTCC 
TGTTCTGTGG TGGATGTCTT TACACAACTC AACCAGAGCT TTGAGATCAT CCGGAAGTTG 
GAATGTCCAG ACCCCAACAT CCTTGCCCAC TACATGAGAA GGTTTGCTAA GACCATTGGG 
AAAGTGCTGA TGCAGTATGC AGACATCTTA TCAAAGAACT TCCCAGCTTA CTGCACAAAG 
GAGAGACTGC CCTGTATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAAGAGCTG GACTCCGAAG CTGCAGACAG TCTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTTAGCA TGGTGTTTGG AAACAGCTTC 
CAGGTGCGGA TTGATGAATG TGTTCGACAA ATGGCTGACA TCCTGGGCCA AGTTCGGGGC 
ACAGGGAATG CATCCCCTAA CGCCAGGGCC TCGGTGGCTC AGGATGCAGA TAGTGTGCTG 
CGACCTCTCA TGGACTTCCT GGATGGCAAC CTCACGCTGT TTGCCACTGT GTGTGAGAAG 
ACAGTTCTGA AGCGGGTCCT GAAGGAGCTC TGGCGGGTGG TGATGAACAC CATGGAGCGG 
GTTATTGTCC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT AACTGCTGCC 
AAGGAGCTGA GCCAGCTTTC CAAACTCAAG GACCACATGG TACGGGAGGA AACACGGAAT 
CTCACTCCAA AGCAGTGTGC TGTCCTCGAC TTGGCCTTGG ACACCATCAA GCAATACTTC 
CATGCAGGAG GCAATGGTCT GAAGAAAACC TTCCTGGAAA AGAGCCCAGA CCTGCAGTCT 
CTGCGTTATG CCCTCTCTCT GTATACACAG ACTACAGACA CTCTCATCAA GACATTTGTG 
CGCTCACAGA CTGCCCAGGG GGCTGGTGTG GATGACCCCG TGGGAGAAGT CTCTATTCAG 
GTGGACTTAT TTACACATCC TGGCACTGGG GAGCATAAGG TCACAGTGAA AGTGGTAGCA 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CTTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA TAAGAAGAGG AAGTTCACAA CCAAGTCTAA AAGTAACAAC 
TGGACTCCCA AGTACAACGA GACGTTTCAC TTCCTCCTGG GAAATGAAGA GGGTCCAGAG 
GCCTATGAAT TGCAGATCTG TGTGAAGGAT TACTGCTTTG CCCGGGAGGA TCGTGTGATA 
GGACTGGCAG TGATGCCCCT GAGGGATGTG GCAGCTAAGG GCAGCTGTGC CTGCTGGTGC 
CCACTGGGCC GGAAGATCCA TATGGATGAG ACAGGCATGA CCATTCTCCG GATTCTGTCT 
CAGAGGAGCA ATGACGAGGT AGCCCGAGAG TTCGTGAAGC TCAAATCAGA GTCTCGATCC 
ACAGAGGAAG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537853.1
CDS	2563..8460
Translation	MKRLLRESEEEIMLTLGPSSSLSPDKVRAETVCSVKSKSTGPTGSLPEDNFPLPCCDSTTSGERDGDLPQLCSFEQQANNQPSLASTACASGSDSRELSPASITNCSDPSERNQARPILPRGPGQRCRYEQQEPLGDVVEYIIRELQGISRLQSEIAELQQHLNQVQGSVDEVSSCVDSVLSEIEGLHVGSSSLGKVCSGEKAQELHVERSREEAILYLYGLPEHDGENTMELVDNFLAKHLCVNGMQCNRYIQEAYRTGTAPAPRPTVVKLVHLEHRDLILQKSILLQSVGVRVATREEPVWSEGCEDPPKESLSCLQQLQDHSWNSLNQDKPALRLETGNRKQMTEPHQIRTQNQQRGPQASEHQGPRLLPKDGLAKQSDVSKLQEEVKGTSGASWVTSDCCGELSLLHPLEGSSSVLISKEEDCEKLQIFKQGRQEHEACKVTKLQSDCSNSIKDSSCLSLSGPLKADMVNTEDKMLGCEAGLDILSPKQLEDLLADKSRRFATLSPDSTMEEVIIGPETLSNMVHIDLNEEENCTAQVLKDVFDKSSRVLADSQEDEDVEIKFHTTKLSRAIQHFRLALQGVFQKLENNGSISPEDLESNESGSQSENSDRLLWTVSSGGAHDCSVESPASQGSESLLSVVSGGVGISIQGDQTSQAPSNFSLASNNSPFANSLLSFPLAPCLGSETCSRPDSPNQGKLSLEQVCAETIYLNKCINNFKNVLREKRLRQKKLLQELVQTASHLSVEDIPSEGNQEAIQISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537790.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537790.4

ORF Insert Sequence:

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGCAGGAACA ACCTCTAGAG GAACCAGGGC CCAGCATTCG GAACTTGGAT 
TTCTGGCCCA AACTTATCAC ACTCATTGTT TCGATCATAG AGGAGGATAA GAATTCCTAC 
ACACCTGTTC TGAGCCAGTT TCCTCAGGAG TTGAACGTAG GAAAAGTCAG TGCAGAAGTG 
ATGTGGCATC TGTTTGCTCA AGACATGAAA TATGCACTGG AGGAACACGA GAAAGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TATGTGCGCG ATCTGCCTGC CCTCCAGGGG CAAGTGCCTG AGTACCCAGC GTGGTTTGAG 
CAGTTTGTGC TACAGTGGCT AGATGAGAAT GAAGATGTGT CACTGGAATT CCTTCGTGGG 
GCCTTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC CTTGTTTTCC 
TGTTCTGTGG TGGATGTCTT TACACAACTC AACCAGAGCT TTGAGATCAT CCGGAAGTTG 
GAATGTCCAG ACCCCAACAT CCTTGCCCAC TACATGAGAA GGTTTGCTAA GACCATTGGG 
AAAGTGCTGA TGCAGTATGC AGACATCTTA TCAAAGAACT TCCCAGCTTA CTGCACAAAG 
GAGAGACTGC CCTGTATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAAGAGCTG GACTCCGAAG CTGCAGACAG TCTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTTAGCA TGGTGTTTGG AAACAGCTTC 
CAGGTGCGGA TTGATGAATG TGTTCGACAA ATGGCTGACA TCCTGGGCCA AGTTCGGGGC 
ACAGGGAATG CATCCCCTAA CGCCAGGGCC TCGGTGGCTC AGGATGCAGA TAGTGTGCTG 
CGACCTCTCA TGGACTTCCT GGATGGCAAC CTCACGCTGT TTGCCACTGT GTGTGAGAAG 
ACAGTTCTGA AGCGGGTCCT GAAGGAGCTC TGGCGGGTGG TGATGAACAC CATGGAGCGG 
GTTATTGTCC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT AACTGCTGCC 
AAGGAGCTGA GCCAGCTTTC CAAACTCAAG GACCACATGG TACGGGAGGA AACACGGAAT 
CTCACTCCAA AGCAGTGTGC TGTCCTCGAC TTGGCCTTGG ACACCATCAA GCAATACTTC 
CATGCAGGAG GCAATGGTCT GAAGAAAACC TTCCTGGAAA AGAGCCCAGA CCTGCAGTCT 
CTGCGTTATG CCCTCTCTCT GTATACACAG ACTACAGACA CTCTCATCAA GACATTTGTG 
CGCTCACAGA CTGCCCAGGG GGCTGGTGTG GATGACCCCG TGGGAGAAGT CTCTATTCAG 
GTGGACTTAT TTACACATCC TGGCACTGGG GAGCATAAGG TCACAGTGAA AGTGGTAGCA 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CTTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA TAAGAAGAGG AAGTTCACAA CCAAGTCTAA AAGTAACAAC 
TGGACTCCCA AGTACAACGA GACGTTTCAC TTCCTCCTGG GAAATGAAGA GGGTCCAGAG 
GCCTATGAAT TGCAGATCTG TGTGAAGGAT TACTGCTTTG CCCGGGAGGA TCGTGTGATA 
GGACTGGCAG TGATGCCCCT GAGGGATGTG GCAGCTAAGG GCAGCTGTGC CTGCTGGTGC 
CCACTGGGCC GGAAGATCCA TATGGATGAG ACAGGCATGA CCATTCTCCG GATTCTGTCT 
CAGAGGAGCA ATGACGAGGT AGCCCGAGAG TTCGTGAAGC TCAAATCAGA GTCTCGATCC 
ACAGAGGAAG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537791.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537854.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant X6, mRNA.

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CloneID	OMu32856
Clone ID Related Accession (Same CDS sequence)	XM_006537791.4 , XM_006537791.3
Accession Version	XM_006537791.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537791.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGAGAC 
AGTGACTACC GCAGTGAGAC CAGCAATAGC GCCCCACCTC CTTACCACAC GACTACCCAG 
CCCAACGCTT CTGTACACCA GTTCCCTGTT CCTGTGCGGC TGCCACAGCA GCTCTTTCTT 
CAAGGCAGTT CCCATGACTC TTGCAATGAC TCTATGCAGA GTTACGACCT TGATTATCCA 
GAGCGTCGGG CCCTCAGCCC TACCAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CAAGGAAGCT CTCTGCTGAG TGAGCTGGAC CAGTATCATG AGCAAGATGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGTCATAGC TATGGCAGCC TCTCCAAAGA TGGCCAGGCT 
GGTTTGGGAG AACAAGAGAA AGCCTTGGAA GTGACATGTG AATCAGAAAA AGAGAAAACA 
GGTGAATCCA AGGAGATGAG AGATGATGCT ACAATCCACC CTCCTTCAGA TCTGGTGCTG 
CATAAGGACC ATGTCCTAGG ACCCCAGGAG AGTCTTCCTG AGGAGACCGC ATCATCGCCA 
TTTACCCAGG CCAGAGCACA CTGGTTCCGA GCGGTTACCA AGGTCCGACT CCAACTGCAG 
GAGATTTCAG ATGATGGTGA CCCCTCCTTG CCTCAGTGGC TCCCAGAAGG GCCAGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGAAGGA AGAAGCCACT GCCACTTGTC 
AGTGATCTGG CTATGTCACT GGTCCAGTCA CGGAAAGCTG GGATTACATC TGCTATGGCT 
ACACGTACCT CTCTCAAGGA TGAAGAACTG AAATCTCATG TGTATAAGAA AACTCTGCAG 
GCCTTAATCT ACCCCATCTC GTGTACCACG CCCCACAACT TTGAGGTCTG GTCTGCCACT 
ACACCTACCT ATTGCTATGA GTGTGAAGGC TTGCTCTGGG GCCTTGCCCG GCAAGGCATG 
CGCTGCAGCG AGTGTGGAGT CAAGTGCCAT GAGAAGTGCC AGGACCTGCT CAATGCTGAC 
TGCCTACAGC GGGCTGCTGA AAAGAGCTCT AAGCATGGAG CTGAGGACCG AACTCAGAAC 
ATTATCATGG CCATGAAGGA CCGTATGAAG ATCCGAGAAC GGAATAAACC AGAGATCTTT 
GAGGTTATCC GGGATGTGTT CACAGTGAGC AAAGTTGCGC ATGTGCAGCA GATGAAAACA 
GTAAAGCAGA GTGTCCTGGA TGGCACCTCC AAGTGGTCAG CCAAAATTAC CATCACGGTG 
GTTTGTGCCC AGGGCCTACA AGCCAAAGAC AAGACAGGAT CCAGTGACCC TTATGTGACT 
GTGCAAGTTG GGAAAACCAA GAAGCGCACC AAGACCATTT TTGGAAATTT GAATCCTGTT 
TGGGAAGAGA AGTTCCATTT TGAGTGCCAC AACTCTTCTG ATCGAATTAA GGTACGCGTG 
TGGGATGAGG ATGATGACAT CAAGTCCAGA GTAAAGCAGC GGCTAAAGCG AGAGTCAGAT 
GATTTCCTTG GCCAAACTAT CATTGAAGTT CGAACGCTGA GTGGAGAGAT GGATGTCTGG 
TACAATCTGG AGAAGAGAAC AGACAAGTCA GCTGTCTCAG GGGCTATCCG ACTTCAAATC 
AGTGTGGAGA TCAAGGGGGA GGAGAAGGTA GCCCCATACC ATGTACAATA TACATGTCTC 
CATGAGAACC TTTTCCATTA CCTCACAGAC ATTCAGGGCA GTGGAGGAGT CTGGATCCCA 
GAGGCTCGGG GGGACGATGC ATGGAAGGTG TACTTTGATG AGACAGCCCA AGAAATTGTG 
GATGAATTCG CTATGCGCTA TGGCATCGAG TCCATTTATC AGGCCATGAC GCACTTTGCA 
TGTTTGTCAT CCAAGTACAT GTGTCCTGGT GTACCAGCCG TGATGAGCAC CTTACTGGCC 
AACATCAATG CCTATTATGC TCACACAACT GCCTCCACTA ACGTCTCTGC ATCTGATCGT 
TTTGCAGCCT CCAACTTTGG TAAAGAAAGA TTTGTAAAAC TGCTGGACCA GCTACACAAC 
TCACTGAGGA TAGACCTCTC TACATACAGG AATAACTTTC CTGCTGGGAG CCCTGAGCGG 
CTTCAGGATT TAAAGTCTAC AGTGGATTTG CTGACCAGCA TTACTTTCTT CAGGATGAAG 
GTGCAAGAAC TTCAGAGCCC TCCAAGAGCC AGCCAGGTGG TAAAGGACTG TGTGAAAGCC 
TGCTTGAACT CTACATATGA ATACATCTTC AACAACTGCC ATGACCTCTA CAGTCACCAG 
TATCAGCTGC AGGAACAACC TCTAGAGGAA CCAGGGCCCA GCATTCGGAA CTTGGATTTC 
TGGCCCAAAC TTATCACACT CATTGTTTCG ATCATAGAGG AGGATAAGAA TTCCTACACA 
CCTGTTCTGA GCCAGTTTCC TCAGGAGTTG AACGTAGGAA AAGTCAGTGC AGAAGTGATG 
TGGCATCTGT TTGCTCAAGA CATGAAATAT GCACTGGAGG AACACGAGAA AGACCGGCTG 
TGTAAGAGTG CTGACTACAT GAACCTGCAC TTCAAGGTGA AGTGGCTCCA CAATGAATAT 
GTGCGCGATC TGCCTGCCCT CCAGGGGCAA GTGCCTGAGT ACCCAGCGTG GTTTGAGCAG 
TTTGTGCTAC AGTGGCTAGA TGAGAATGAA GATGTGTCAC TGGAATTCCT TCGTGGGGCC 
TTGGAACGAG ATAAGAAGGA TGGGTTCCAG CAGACATCAG AGCATGCCTT GTTTTCCTGT 
TCTGTGGTGG ATGTCTTTAC ACAACTCAAC CAGAGCTTTG AGATCATCCG GAAGTTGGAA 
TGTCCAGACC CCAACATCCT TGCCCACTAC ATGAGAAGGT TTGCTAAGAC CATTGGGAAA 
GTGCTGATGC AGTATGCAGA CATCTTATCA AAGAACTTCC CAGCTTACTG CACAAAGGAG 
AGACTGCCCT GTATCCTGAT GAACAACATG CAGCAACTGA GGGTCCAGCT GGAGAAAATG 
TTTGAGGCCA TGGGAGGCAA AGAGCTGGAC TCCGAAGCTG CAGACAGTCT GAAGGAGCTG 
CAGGTGAAAC TGAATACAGT TCTGGATGAG CTTAGCATGG TGTTTGGAAA CAGCTTCCAG 
GTGCGGATTG ATGAATGTGT TCGACAAATG GCTGACATCC TGGGCCAAGT TCGGGGCACA 
GGGAATGCAT CCCCTAACGC CAGGGCCTCG GTGGCTCAGG ATGCAGATAG TGTGCTGCGA 
CCTCTCATGG ACTTCCTGGA TGGCAACCTC ACGCTGTTTG CCACTGTGTG TGAGAAGACA 
GTTCTGAAGC GGGTCCTGAA GGAGCTCTGG CGGGTGGTGA TGAACACCAT GGAGCGGGTT 
ATTGTCCTGC CCCCACTCAC TGACCAGACG GGCACCCAGC TGATCTTAAC TGCTGCCAAG 
GAGCTGAGCC AGCTTTCCAA ACTCAAGGAC CACATGGTAC GGGAGGAAAC ACGGAATCTC 
ACTCCAAAGC AGTGTGCTGT CCTCGACTTG GCCTTGGACA CCATCAAGCA ATACTTCCAT 
GCAGGAGGCA ATGGTCTGAA GAAAACCTTC CTGGAAAAGA GCCCAGACCT GCAGTCTCTG 
CGTTATGCCC TCTCTCTGTA TACACAGACT ACAGACACTC TCATCAAGAC ATTTGTGCGC 
TCACAGACTG CCCAGGTACA TGATGGGAAA GGTATTAGGT TTACTGCTAA CGAGGACATT 
CGGCCAGAAA AGGGGGCTGG TGTGGATGAC CCCGTGGGAG AAGTCTCTAT TCAGGTGGAC 
TTATTTACAC ATCCTGGCAC TGGGGAGCAT AAGGTCACAG TGAAAGTGGT AGCAGCCAAT 
GACCTCAAGT GGCAGACAGC GGGTATGTTC CGGCCTTTTG TGGAAGTGAC CATGGTTGGC 
CCACACCAAA GTGATAAGAA GAGGAAGTTC ACAACCAAGT CTAAAAGTAA CAACTGGACT 
CCCAAGTACA ACGAGACGTT TCACTTCCTC CTGGGAAATG AAGAGGGTCC AGAGGCCTAT 
GAATTGCAGA TCTGTGTGAA GGATTACTGC TTTGCCCGGG AGGATCGTGT GATAGGACTG 
GCAGTGATGC CCCTGAGGGA TGTGGCAGCT AAGGGCAGCT GTGCCTGCTG GTGCCCACTG 
GGCCGGAAGA TCCATATGGA TGAGACAGGC ATGACCATTC TCCGGATTCT GTCTCAGAGG 
AGCAATGACG AGGTAGCCCG AGAGTTCGTG AAGCTCAAAT CAGAGTCTCG ATCCACAGAG 
GAAGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537854.1
CDS	449..5320
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCCHWTYLGWGEHQTFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537791.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537791.4

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGAGAC 
AGTGACTACC GCAGTGAGAC CAGCAATAGC GCCCCACCTC CTTACCACAC GACTACCCAG 
CCCAACGCTT CTGTACACCA GTTCCCTGTT CCTGTGCGGC TGCCACAGCA GCTCTTTCTT 
CAAGGCAGTT CCCATGACTC TTGCAATGAC TCTATGCAGA GTTACGACCT TGATTATCCA 
GAGCGTCGGG CCCTCAGCCC TACCAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CAAGGAAGCT CTCTGCTGAG TGAGCTGGAC CAGTATCATG AGCAAGATGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGTCATAGC TATGGCAGCC TCTCCAAAGA TGGCCAGGCT 
GGTTTGGGAG AACAAGAGAA AGCCTTGGAA GTGACATGTG AATCAGAAAA AGAGAAAACA 
GGTGAATCCA AGGAGATGAG AGATGATGCT ACAATCCACC CTCCTTCAGA TCTGGTGCTG 
CATAAGGACC ATGTCCTAGG ACCCCAGGAG AGTCTTCCTG AGGAGACCGC ATCATCGCCA 
TTTACCCAGG CCAGAGCACA CTGGTTCCGA GCGGTTACCA AGGTCCGACT CCAACTGCAG 
GAGATTTCAG ATGATGGTGA CCCCTCCTTG CCTCAGTGGC TCCCAGAAGG GCCAGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGAAGGA AGAAGCCACT GCCACTTGTC 
AGTGATCTGG CTATGTCACT GGTCCAGTCA CGGAAAGCTG GGATTACATC TGCTATGGCT 
ACACGTACCT CTCTCAAGGA TGAAGAACTG AAATCTCATG TGTATAAGAA AACTCTGCAG 
GCCTTAATCT ACCCCATCTC GTGTACCACG CCCCACAACT TTGAGGTCTG GTCTGCCACT 
ACACCTACCT ATTGCTATGA GTGTGAAGGC TTGCTCTGGG GCCTTGCCCG GCAAGGCATG 
CGCTGCAGCG AGTGTGGAGT CAAGTGCCAT GAGAAGTGCC AGGACCTGCT CAATGCTGAC 
TGCCTACAGC GGGCTGCTGA AAAGAGCTCT AAGCATGGAG CTGAGGACCG AACTCAGAAC 
ATTATCATGG CCATGAAGGA CCGTATGAAG ATCCGAGAAC GGAATAAACC AGAGATCTTT 
GAGGTTATCC GGGATGTGTT CACAGTGAGC AAAGTTGCGC ATGTGCAGCA GATGAAAACA 
GTAAAGCAGA GTGTCCTGGA TGGCACCTCC AAGTGGTCAG CCAAAATTAC CATCACGGTG 
GTTTGTGCCC AGGGCCTACA AGCCAAAGAC AAGACAGGAT CCAGTGACCC TTATGTGACT 
GTGCAAGTTG GGAAAACCAA GAAGCGCACC AAGACCATTT TTGGAAATTT GAATCCTGTT 
TGGGAAGAGA AGTTCCATTT TGAGTGCCAC AACTCTTCTG ATCGAATTAA GGTACGCGTG 
TGGGATGAGG ATGATGACAT CAAGTCCAGA GTAAAGCAGC GGCTAAAGCG AGAGTCAGAT 
GATTTCCTTG GCCAAACTAT CATTGAAGTT CGAACGCTGA GTGGAGAGAT GGATGTCTGG 
TACAATCTGG AGAAGAGAAC AGACAAGTCA GCTGTCTCAG GGGCTATCCG ACTTCAAATC 
AGTGTGGAGA TCAAGGGGGA GGAGAAGGTA GCCCCATACC ATGTACAATA TACATGTCTC 
CATGAGAACC TTTTCCATTA CCTCACAGAC ATTCAGGGCA GTGGAGGAGT CTGGATCCCA 
GAGGCTCGGG GGGACGATGC ATGGAAGGTG TACTTTGATG AGACAGCCCA AGAAATTGTG 
GATGAATTCG CTATGCGCTA TGGCATCGAG TCCATTTATC AGGCCATGAC GCACTTTGCA 
TGTTTGTCAT CCAAGTACAT GTGTCCTGGT GTACCAGCCG TGATGAGCAC CTTACTGGCC 
AACATCAATG CCTATTATGC TCACACAACT GCCTCCACTA ACGTCTCTGC ATCTGATCGT 
TTTGCAGCCT CCAACTTTGG TAAAGAAAGA TTTGTAAAAC TGCTGGACCA GCTACACAAC 
TCACTGAGGA TAGACCTCTC TACATACAGG AATAACTTTC CTGCTGGGAG CCCTGAGCGG 
CTTCAGGATT TAAAGTCTAC AGTGGATTTG CTGACCAGCA TTACTTTCTT CAGGATGAAG 
GTGCAAGAAC TTCAGAGCCC TCCAAGAGCC AGCCAGGTGG TAAAGGACTG TGTGAAAGCC 
TGCTTGAACT CTACATATGA ATACATCTTC AACAACTGCC ATGACCTCTA CAGTCACCAG 
TATCAGCTGC AGGAACAACC TCTAGAGGAA CCAGGGCCCA GCATTCGGAA CTTGGATTTC 
TGGCCCAAAC TTATCACACT CATTGTTTCG ATCATAGAGG AGGATAAGAA TTCCTACACA 
CCTGTTCTGA GCCAGTTTCC TCAGGAGTTG AACGTAGGAA AAGTCAGTGC AGAAGTGATG 
TGGCATCTGT TTGCTCAAGA CATGAAATAT GCACTGGAGG AACACGAGAA AGACCGGCTG 
TGTAAGAGTG CTGACTACAT GAACCTGCAC TTCAAGGTGA AGTGGCTCCA CAATGAATAT 
GTGCGCGATC TGCCTGCCCT CCAGGGGCAA GTGCCTGAGT ACCCAGCGTG GTTTGAGCAG 
TTTGTGCTAC AGTGGCTAGA TGAGAATGAA GATGTGTCAC TGGAATTCCT TCGTGGGGCC 
TTGGAACGAG ATAAGAAGGA TGGGTTCCAG CAGACATCAG AGCATGCCTT GTTTTCCTGT 
TCTGTGGTGG ATGTCTTTAC ACAACTCAAC CAGAGCTTTG AGATCATCCG GAAGTTGGAA 
TGTCCAGACC CCAACATCCT TGCCCACTAC ATGAGAAGGT TTGCTAAGAC CATTGGGAAA 
GTGCTGATGC AGTATGCAGA CATCTTATCA AAGAACTTCC CAGCTTACTG CACAAAGGAG 
AGACTGCCCT GTATCCTGAT GAACAACATG CAGCAACTGA GGGTCCAGCT GGAGAAAATG 
TTTGAGGCCA TGGGAGGCAA AGAGCTGGAC TCCGAAGCTG CAGACAGTCT GAAGGAGCTG 
CAGGTGAAAC TGAATACAGT TCTGGATGAG CTTAGCATGG TGTTTGGAAA CAGCTTCCAG 
GTGCGGATTG ATGAATGTGT TCGACAAATG GCTGACATCC TGGGCCAAGT TCGGGGCACA 
GGGAATGCAT CCCCTAACGC CAGGGCCTCG GTGGCTCAGG ATGCAGATAG TGTGCTGCGA 
CCTCTCATGG ACTTCCTGGA TGGCAACCTC ACGCTGTTTG CCACTGTGTG TGAGAAGACA 
GTTCTGAAGC GGGTCCTGAA GGAGCTCTGG CGGGTGGTGA TGAACACCAT GGAGCGGGTT 
ATTGTCCTGC CCCCACTCAC TGACCAGACG GGCACCCAGC TGATCTTAAC TGCTGCCAAG 
GAGCTGAGCC AGCTTTCCAA ACTCAAGGAC CACATGGTAC GGGAGGAAAC ACGGAATCTC 
ACTCCAAAGC AGTGTGCTGT CCTCGACTTG GCCTTGGACA CCATCAAGCA ATACTTCCAT 
GCAGGAGGCA ATGGTCTGAA GAAAACCTTC CTGGAAAAGA GCCCAGACCT GCAGTCTCTG 
CGTTATGCCC TCTCTCTGTA TACACAGACT ACAGACACTC TCATCAAGAC ATTTGTGCGC 
TCACAGACTG CCCAGGTACA TGATGGGAAA GGTATTAGGT TTACTGCTAA CGAGGACATT 
CGGCCAGAAA AGGGGGCTGG TGTGGATGAC CCCGTGGGAG AAGTCTCTAT TCAGGTGGAC 
TTATTTACAC ATCCTGGCAC TGGGGAGCAT AAGGTCACAG TGAAAGTGGT AGCAGCCAAT 
GACCTCAAGT GGCAGACAGC GGGTATGTTC CGGCCTTTTG TGGAAGTGAC CATGGTTGGC 
CCACACCAAA GTGATAAGAA GAGGAAGTTC ACAACCAAGT CTAAAAGTAA CAACTGGACT 
CCCAAGTACA ACGAGACGTT TCACTTCCTC CTGGGAAATG AAGAGGGTCC AGAGGCCTAT 
GAATTGCAGA TCTGTGTGAA GGATTACTGC TTTGCCCGGG AGGATCGTGT GATAGGACTG 
GCAGTGATGC CCCTGAGGGA TGTGGCAGCT AAGGGCAGCT GTGCCTGCTG GTGCCCACTG 
GGCCGGAAGA TCCATATGGA TGAGACAGGC ATGACCATTC TCCGGATTCT GTCTCAGAGG 
AGCAATGACG AGGTAGCCCG AGAGTTCGTG AAGCTCAAAT CAGAGTCTCG ATCCACAGAG 
GAAGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537794.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537857.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant X8, mRNA.

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CloneID	OMu32859
Clone ID Related Accession (Same CDS sequence)	XM_006537794.4 , XM_006537794.3
Accession Version	XM_006537794.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537794.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTCCTT TGAAGACCCT 
GATAGTGCCG TTGATGACCG AGACAGTGAC TACCGCAGTG AGACCAGCAA TAGCGCCCCA 
CCTCCTTACC ACACGACTAC CCAGCCCAAC GCTTCTGTAC ACCAGTTCCC TGTTCCTGTG 
CGGCTGCCAC AGCAGCTCTT TCTTCAAGGC AGTTCCCATG ACTCTTGCAA TGACTCTATG 
CAGAGTTACG ACCTTGATTA TCCAGAGCGT CGGGCCCTCA GCCCTACCAG CAGTAGTAGG 
TATGGCTCCT CCTGTAATGT GAGTCAAGGA AGCTCTCTGC TGAGTGAGCT GGACCAGTAT 
CATGAGCAAG ATGATGATGG TCGGGAGAGG GACTCCATTC ATTCTAGTCA TAGCTATGGC 
AGCCTCTCCA AAGATGGCCA GGCTGGTTTG GGAGAACAAG AGAAAGCCTT GGAAGTGACA 
TGTGAATCAG AAAAAGAGAA AACAGGTGAA TCCAAGGAGA TGAGAGATGA TGCTACAATC 
CACCCTCCTT CAGATCTGGT GCTGCATAAG GACCATGTCC TAGGACCCCA GGAGAGTCTT 
CCTGAGGAGA CCGCATCATC GCCATTTACC CAGGCCAGAG CACACTGGTT CCGAGCGGTT 
ACCAAGGTCC GACTCCAACT GCAGGAGATT TCAGATGATG GTGACCCCTC CTTGCCTCAG 
TGGCTCCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGA 
AGGAAGAAGC CACTGCCACT TGTCAGTGAT CTGGCTATGT CACTGGTCCA GTCACGGAAA 
GCTGGGATTA CATCTGCTAT GGCTACACGT ACCTCTCTCA AGGATGAAGA ACTGAAATCT 
CATGTGTATA AGAAAACTCT GCAGGCCTTA ATCTACCCCA TCTCGTGTAC CACGCCCCAC 
AACTTTGAGG TCTGGTCTGC CACTACACCT ACCTATTGCT ATGAGTGTGA AGGCTTGCTC 
TGGGGCCTTG CCCGGCAAGG CATGCGCTGC AGCGAGTGTG GAGTCAAGTG CCATGAGAAG 
TGCCAGGACC TGCTCAATGC TGACTGCCTA CAGCGGGCTG CTGAAAAGAG CTCTAAGCAT 
GGAGCTGAGG ACCGAACTCA GAACATTATC ATGGCCATGA AGGACCGTAT GAAGATCCGA 
GAACGGAATA AACCAGAGAT CTTTGAGGTT ATCCGGGATG TGTTCACAGT GAGCAAAGTT 
GCGCATGTGC AGCAGATGAA AACAGTAAAG CAGAGTGTCC TGGATGGCAC CTCCAAGTGG 
TCAGCCAAAA TTACCATCAC GGTGGTTTGT GCCCAGGGCC TACAAGCCAA AGACAAGACA 
GGATCCAGTG ACCCTTATGT GACTGTGCAA GTTGGGAAAA CCAAGAAGCG CACCAAGACC 
ATTTTTGGAA ATTTGAATCC TGTTTGGGAA GAGAAGTTCC ATTTTGAGTG CCACAACTCT 
TCTGATCGAA TTAAGGTACG CGTGTGGGAT GAGGATGATG ACATCAAGTC CAGAGTAAAG 
CAGCGGCTAA AGCGAGAGTC AGATGATTTC CTTGGCCAAA CTATCATTGA AGTTCGAACG 
CTGAGTGGAG AGATGGATGT CTGGTACAAT CTGGAGAAGA GAACAGACAA GTCAGCTGTC 
TCAGGGGCTA TCCGACTTCA AATCAGTGTG GAGATCAAGG GGGAGGAGAA GGTAGCCCCA 
TACCATGTAC AATATACATG TCTCCATGAG AACCTTTTCC ATTACCTCAC AGACATTCAG 
GGCAGTGGAG GAGTCTGGAT CCCAGAGGCT CGGGGGGACG ATGCATGGAA GGTGTACTTT 
GATGAGACAG CCCAAGAAAT TGTGGATGAA TTCGCTATGC GCTATGGCAT CGAGTCCATT 
TATCAGGCCA TGACGCACTT TGCATGTTTG TCATCCAAGT ACATGTGTCC TGGTGTACCA 
GCCGTGATGA GCACCTTACT GGCCAACATC AATGCCTATT ATGCTCACAC AACTGCCTCC 
ACTAACGTCT CTGCATCTGA TCGTTTTGCA GCCTCCAACT TTGGTAAAGA AAGATTTGTA 
AAACTGCTGG ACCAGCTACA CAACTCACTG AGGATAGACC TCTCTACATA CAGGAATAAC 
TTTCCTGCTG GGAGCCCTGA GCGGCTTCAG GATTTAAAGT CTACAGTGGA TTTGCTGACC 
AGCATTACTT TCTTCAGGAT GAAGGTGCAA GAACTTCAGA GCCCTCCAAG AGCCAGCCAG 
GTGGTAAAGG ACTGTGTGAA AGCCTGCTTG AACTCTACAT ATGAATACAT CTTCAACAAC 
TGCCATGACC TCTACAGTCA CCAGTATCAG CTGCAGGAAC AACCTCTAGA GGAACCAGGG 
CCCAGCATTC GGAACTTGGA TTTCTGGCCC AAACTTATCA CACTCATTGT TTCGATCATA 
GAGGAGGATA AGAATTCCTA CACACCTGTT CTGAGCCAGT TTCCTCAGGA GTTGAACGTA 
GGAAAAGTCA GTGCAGAAGT GATGTGGCAT CTGTTTGCTC AAGACATGAA ATATGCACTG 
GAGGAACACG AGAAAGACCG GCTGTGTAAG AGTGCTGACT ACATGAACCT GCACTTCAAG 
GTGAAGTGGC TCCACAATGA ATATGTGCGC GATCTGCCTG CCCTCCAGGG GCAAGTGCCT 
GAGTACCCAG CGTGGTTTGA GCAGTTTGTG CTACAGTGGC TAGATGAGAA TGAAGATGTG 
TCACTGGAAT TCCTTCGTGG GGCCTTGGAA CGAGATAAGA AGGATGGGTT CCAGCAGACA 
TCAGAGCATG CCTTGTTTTC CTGTTCTGTG GTGGATGTCT TTACACAACT CAACCAGAGC 
TTTGAGATCA TCCGGAAGTT GGAATGTCCA GACCCCAACA TCCTTGCCCA CTACATGAGA 
AGGTTTGCTA AGACCATTGG GAAAGTGCTG ATGCAGTATG CAGACATCTT ATCAAAGAAC 
TTCCCAGCTT ACTGCACAAA GGAGAGACTG CCCTGTATCC TGATGAACAA CATGCAGCAA 
CTGAGGGTCC AGCTGGAGAA AATGTTTGAG GCCATGGGAG GCAAAGAGCT GGACTCCGAA 
GCTGCAGACA GTCTGAAGGA GCTGCAGGTG AAACTGAATA CAGTTCTGGA TGAGCTTAGC 
ATGGTGTTTG GAAACAGCTT CCAGGTGCGG ATTGATGAAT GTGTTCGACA AATGGCTGAC 
ATCCTGGGCC AAGTTCGGGG CACAGGGAAT GCATCCCCTA ACGCCAGGGC CTCGGTGGCT 
CAGGATGCAG ATAGTGTGCT GCGACCTCTC ATGGACTTCC TGGATGGCAA CCTCACGCTG 
TTTGCCACTG TGTGTGAGAA GACAGTTCTG AAGCGGGTCC TGAAGGAGCT CTGGCGGGTG 
GTGATGAACA CCATGGAGCG GGTTATTGTC CTGCCCCCAC TCACTGACCA GACGGGCACC 
CAGCTGATCT TAACTGCTGC CAAGGAGCTG AGCCAGCTTT CCAAACTCAA GGACCACATG 
GTACGGGAGG AAACACGGAA TCTCACTCCA AAGCAGTGTG CTGTCCTCGA CTTGGCCTTG 
GACACCATCA AGCAATACTT CCATGCAGGA GGCAATGGTC TGAAGAAAAC CTTCCTGGAA 
AAGAGCCCAG ACCTGCAGTC TCTGCGTTAT GCCCTCTCTC TGTATACACA GACTACAGAC 
ACTCTCATCA AGACATTTGT GCGCTCACAG ACTGCCCAGG TACATGATGG GAAAGGTATT 
AGGTTTACTG CTAACGAGGA CATTCGGCCA GAAAAGGGGG CTGGTGTGGA TGACCCCGTG 
GGAGAAGTCT CTATTCAGGT GGACTTATTT ACACATCCTG GCACTGGGGA GCATAAGGTC 
ACAGTGAAAG TGGTAGCAGC CAATGACCTC AAGTGGCAGA CAGCGGGTAT GTTCCGGCCT 
TTTGTGGAAG TGACCATGGT TGGCCCACAC CAAAGTGATA AGAAGAGGAA GTTCACAACC 
AAGTCTAAAA GTAACAACTG GACTCCCAAG TACAACGAGA CGTTTCACTT CCTCCTGGGA 
AATGAAGAGG GTCCAGAGGC CTATGAATTG CAGATCTGTG TGAAGGATTA CTGCTTTGCC 
CGGGAGGATC GTGTGATAGG ACTGGCAGTG ATGCCCCTGA GGGATGTGGC AGCTAAGGGC 
AGCTGTGCCT GCTGGTGCCC ACTGGGCCGG AAGATCCATA TGGATGAGAC AGGCATGACC 
ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT GACGAGGTAG CCCGAGAGTT CGTGAAGCTC 
AAATCAGAGT CTCGATCCAC AGAGGAAGGG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537857.1
CDS	449..5284
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537794.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537794.4

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTCCTT TGAAGACCCT 
GATAGTGCCG TTGATGACCG AGACAGTGAC TACCGCAGTG AGACCAGCAA TAGCGCCCCA 
CCTCCTTACC ACACGACTAC CCAGCCCAAC GCTTCTGTAC ACCAGTTCCC TGTTCCTGTG 
CGGCTGCCAC AGCAGCTCTT TCTTCAAGGC AGTTCCCATG ACTCTTGCAA TGACTCTATG 
CAGAGTTACG ACCTTGATTA TCCAGAGCGT CGGGCCCTCA GCCCTACCAG CAGTAGTAGG 
TATGGCTCCT CCTGTAATGT GAGTCAAGGA AGCTCTCTGC TGAGTGAGCT GGACCAGTAT 
CATGAGCAAG ATGATGATGG TCGGGAGAGG GACTCCATTC ATTCTAGTCA TAGCTATGGC 
AGCCTCTCCA AAGATGGCCA GGCTGGTTTG GGAGAACAAG AGAAAGCCTT GGAAGTGACA 
TGTGAATCAG AAAAAGAGAA AACAGGTGAA TCCAAGGAGA TGAGAGATGA TGCTACAATC 
CACCCTCCTT CAGATCTGGT GCTGCATAAG GACCATGTCC TAGGACCCCA GGAGAGTCTT 
CCTGAGGAGA CCGCATCATC GCCATTTACC CAGGCCAGAG CACACTGGTT CCGAGCGGTT 
ACCAAGGTCC GACTCCAACT GCAGGAGATT TCAGATGATG GTGACCCCTC CTTGCCTCAG 
TGGCTCCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGA 
AGGAAGAAGC CACTGCCACT TGTCAGTGAT CTGGCTATGT CACTGGTCCA GTCACGGAAA 
GCTGGGATTA CATCTGCTAT GGCTACACGT ACCTCTCTCA AGGATGAAGA ACTGAAATCT 
CATGTGTATA AGAAAACTCT GCAGGCCTTA ATCTACCCCA TCTCGTGTAC CACGCCCCAC 
AACTTTGAGG TCTGGTCTGC CACTACACCT ACCTATTGCT ATGAGTGTGA AGGCTTGCTC 
TGGGGCCTTG CCCGGCAAGG CATGCGCTGC AGCGAGTGTG GAGTCAAGTG CCATGAGAAG 
TGCCAGGACC TGCTCAATGC TGACTGCCTA CAGCGGGCTG CTGAAAAGAG CTCTAAGCAT 
GGAGCTGAGG ACCGAACTCA GAACATTATC ATGGCCATGA AGGACCGTAT GAAGATCCGA 
GAACGGAATA AACCAGAGAT CTTTGAGGTT ATCCGGGATG TGTTCACAGT GAGCAAAGTT 
GCGCATGTGC AGCAGATGAA AACAGTAAAG CAGAGTGTCC TGGATGGCAC CTCCAAGTGG 
TCAGCCAAAA TTACCATCAC GGTGGTTTGT GCCCAGGGCC TACAAGCCAA AGACAAGACA 
GGATCCAGTG ACCCTTATGT GACTGTGCAA GTTGGGAAAA CCAAGAAGCG CACCAAGACC 
ATTTTTGGAA ATTTGAATCC TGTTTGGGAA GAGAAGTTCC ATTTTGAGTG CCACAACTCT 
TCTGATCGAA TTAAGGTACG CGTGTGGGAT GAGGATGATG ACATCAAGTC CAGAGTAAAG 
CAGCGGCTAA AGCGAGAGTC AGATGATTTC CTTGGCCAAA CTATCATTGA AGTTCGAACG 
CTGAGTGGAG AGATGGATGT CTGGTACAAT CTGGAGAAGA GAACAGACAA GTCAGCTGTC 
TCAGGGGCTA TCCGACTTCA AATCAGTGTG GAGATCAAGG GGGAGGAGAA GGTAGCCCCA 
TACCATGTAC AATATACATG TCTCCATGAG AACCTTTTCC ATTACCTCAC AGACATTCAG 
GGCAGTGGAG GAGTCTGGAT CCCAGAGGCT CGGGGGGACG ATGCATGGAA GGTGTACTTT 
GATGAGACAG CCCAAGAAAT TGTGGATGAA TTCGCTATGC GCTATGGCAT CGAGTCCATT 
TATCAGGCCA TGACGCACTT TGCATGTTTG TCATCCAAGT ACATGTGTCC TGGTGTACCA 
GCCGTGATGA GCACCTTACT GGCCAACATC AATGCCTATT ATGCTCACAC AACTGCCTCC 
ACTAACGTCT CTGCATCTGA TCGTTTTGCA GCCTCCAACT TTGGTAAAGA AAGATTTGTA 
AAACTGCTGG ACCAGCTACA CAACTCACTG AGGATAGACC TCTCTACATA CAGGAATAAC 
TTTCCTGCTG GGAGCCCTGA GCGGCTTCAG GATTTAAAGT CTACAGTGGA TTTGCTGACC 
AGCATTACTT TCTTCAGGAT GAAGGTGCAA GAACTTCAGA GCCCTCCAAG AGCCAGCCAG 
GTGGTAAAGG ACTGTGTGAA AGCCTGCTTG AACTCTACAT ATGAATACAT CTTCAACAAC 
TGCCATGACC TCTACAGTCA CCAGTATCAG CTGCAGGAAC AACCTCTAGA GGAACCAGGG 
CCCAGCATTC GGAACTTGGA TTTCTGGCCC AAACTTATCA CACTCATTGT TTCGATCATA 
GAGGAGGATA AGAATTCCTA CACACCTGTT CTGAGCCAGT TTCCTCAGGA GTTGAACGTA 
GGAAAAGTCA GTGCAGAAGT GATGTGGCAT CTGTTTGCTC AAGACATGAA ATATGCACTG 
GAGGAACACG AGAAAGACCG GCTGTGTAAG AGTGCTGACT ACATGAACCT GCACTTCAAG 
GTGAAGTGGC TCCACAATGA ATATGTGCGC GATCTGCCTG CCCTCCAGGG GCAAGTGCCT 
GAGTACCCAG CGTGGTTTGA GCAGTTTGTG CTACAGTGGC TAGATGAGAA TGAAGATGTG 
TCACTGGAAT TCCTTCGTGG GGCCTTGGAA CGAGATAAGA AGGATGGGTT CCAGCAGACA 
TCAGAGCATG CCTTGTTTTC CTGTTCTGTG GTGGATGTCT TTACACAACT CAACCAGAGC 
TTTGAGATCA TCCGGAAGTT GGAATGTCCA GACCCCAACA TCCTTGCCCA CTACATGAGA 
AGGTTTGCTA AGACCATTGG GAAAGTGCTG ATGCAGTATG CAGACATCTT ATCAAAGAAC 
TTCCCAGCTT ACTGCACAAA GGAGAGACTG CCCTGTATCC TGATGAACAA CATGCAGCAA 
CTGAGGGTCC AGCTGGAGAA AATGTTTGAG GCCATGGGAG GCAAAGAGCT GGACTCCGAA 
GCTGCAGACA GTCTGAAGGA GCTGCAGGTG AAACTGAATA CAGTTCTGGA TGAGCTTAGC 
ATGGTGTTTG GAAACAGCTT CCAGGTGCGG ATTGATGAAT GTGTTCGACA AATGGCTGAC 
ATCCTGGGCC AAGTTCGGGG CACAGGGAAT GCATCCCCTA ACGCCAGGGC CTCGGTGGCT 
CAGGATGCAG ATAGTGTGCT GCGACCTCTC ATGGACTTCC TGGATGGCAA CCTCACGCTG 
TTTGCCACTG TGTGTGAGAA GACAGTTCTG AAGCGGGTCC TGAAGGAGCT CTGGCGGGTG 
GTGATGAACA CCATGGAGCG GGTTATTGTC CTGCCCCCAC TCACTGACCA GACGGGCACC 
CAGCTGATCT TAACTGCTGC CAAGGAGCTG AGCCAGCTTT CCAAACTCAA GGACCACATG 
GTACGGGAGG AAACACGGAA TCTCACTCCA AAGCAGTGTG CTGTCCTCGA CTTGGCCTTG 
GACACCATCA AGCAATACTT CCATGCAGGA GGCAATGGTC TGAAGAAAAC CTTCCTGGAA 
AAGAGCCCAG ACCTGCAGTC TCTGCGTTAT GCCCTCTCTC TGTATACACA GACTACAGAC 
ACTCTCATCA AGACATTTGT GCGCTCACAG ACTGCCCAGG TACATGATGG GAAAGGTATT 
AGGTTTACTG CTAACGAGGA CATTCGGCCA GAAAAGGGGG CTGGTGTGGA TGACCCCGTG 
GGAGAAGTCT CTATTCAGGT GGACTTATTT ACACATCCTG GCACTGGGGA GCATAAGGTC 
ACAGTGAAAG TGGTAGCAGC CAATGACCTC AAGTGGCAGA CAGCGGGTAT GTTCCGGCCT 
TTTGTGGAAG TGACCATGGT TGGCCCACAC CAAAGTGATA AGAAGAGGAA GTTCACAACC 
AAGTCTAAAA GTAACAACTG GACTCCCAAG TACAACGAGA CGTTTCACTT CCTCCTGGGA 
AATGAAGAGG GTCCAGAGGC CTATGAATTG CAGATCTGTG TGAAGGATTA CTGCTTTGCC 
CGGGAGGATC GTGTGATAGG ACTGGCAGTG ATGCCCCTGA GGGATGTGGC AGCTAAGGGC 
AGCTGTGCCT GCTGGTGCCC ACTGGGCCGG AAGATCCATA TGGATGAGAC AGGCATGACC 
ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT GACGAGGTAG CCCGAGAGTT CGTGAAGCTC 
AAATCAGAGT CTCGATCCAC AGAGGAAGGG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537795.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537858.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant X9, mRNA.

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CloneID	OMu32860
Clone ID Related Accession (Same CDS sequence)	XM_006537795.3 , XM_006537795.4
Accession Version	XM_006537795.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537795.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTCCTT TGAAGACCCT 
GATAGTGCCG TTGATGACCG AGACAGTGAC TACCGCAGTG AGACCAGCAA TAGCGCCCCA 
CCTCCTTACC ACACGACTAC CCAGCCCAAC GCTTCTGTAC ACCAGTTCCC TGTTCCTGTG 
CGGCTGCCAC AGCAGCTCTT TCTTCAAGGC AGTTCCCATG ACTCTTGCAA TGACTCTATG 
CAGAGTTACG ACCTTGATTA TCCAGAGCGT CGGGCCCTCA GCCCTACCAG CAGTAGTAGG 
TATGGCTCCT CCTGTAATGT GAGTCAAGGA AGCTCTCTGC TGAGTGAGCT GGACCAGTAT 
CATGAGCAAG ATGATGATGG TCGGGAGAGG GACTCCATTC ATTCTAGTCA TAGCTATGGC 
AGCCTCTCCA AAGATGGCCA GGCTGGTTTG GGAGAACAAG AGAAAGCCTT GGAAGTGACA 
TGTGAATCAG AAAAAGAGAA AACAGGTGAA TCCAAGGAGA TGAGAGATGA TGCTACAATC 
CACCCTCCTT CAGATCTGGT GCTGCATAAG GACCATGTCC TAGGACCCCA GGAGAGTCTT 
CCTGAGGAGA CCGCATCATC GCCATTTACC CAGGCCAGAG CACACTGGTT CCGAGCGGTT 
ACCAAGGTCC GACTCCAACT GCAGGAGATT TCAGATGATG GTGACCCCTC CTTGCCTCAG 
TGGCTCCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGA 
AGGAAGAAGC CACTGCCACT TGTCAGTGAT CTGTCACTGG TCCAGTCACG GAAAGCTGGG 
ATTACATCTG CTATGGCTAC ACGTACCTCT CTCAAGGATG AAGAACTGAA ATCTCATGTG 
TATAAGAAAA CTCTGCAGGC CTTAATCTAC CCCATCTCGT GTACCACGCC CCACAACTTT 
GAGGTCTGGT CTGCCACTAC ACCTACCTAT TGCTATGAGT GTGAAGGCTT GCTCTGGGGC 
CTTGCCCGGC AAGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG 
GACCTGCTCA ATGCTGACTG CCTACAGCGG GCTGCTGAAA AGAGCTCTAA GCATGGAGCT 
GAGGACCGAA CTCAGAACAT TATCATGGCC ATGAAGGACC GTATGAAGAT CCGAGAACGG 
AATAAACCAG AGATCTTTGA GGTTATCCGG GATGTGTTCA CAGTGAGCAA AGTTGCGCAT 
GTGCAGCAGA TGAAAACAGT AAAGCAGAGT GTCCTGGATG GCACCTCCAA GTGGTCAGCC 
AAAATTACCA TCACGGTGGT TTGTGCCCAG GGCCTACAAG CCAAAGACAA GACAGGATCC 
AGTGACCCTT ATGTGACTGT GCAAGTTGGG AAAACCAAGA AGCGCACCAA GACCATTTTT 
GGAAATTTGA ATCCTGTTTG GGAAGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAT 
CGAATTAAGG TACGCGTGTG GGATGAGGAT GATGACATCA AGTCCAGAGT AAAGCAGCGG 
CTAAAGCGAG AGTCAGATGA TTTCCTTGGC CAAACTATCA TTGAAGTTCG AACGCTGAGT 
GGAGAGATGG ATGTCTGGTA CAATCTGGAG AAGAGAACAG ACAAGTCAGC TGTCTCAGGG 
GCTATCCGAC TTCAAATCAG TGTGGAGATC AAGGGGGAGG AGAAGGTAGC CCCATACCAT 
GTACAATATA CATGTCTCCA TGAGAACCTT TTCCATTACC TCACAGACAT TCAGGGCAGT 
GGAGGAGTCT GGATCCCAGA GGCTCGGGGG GACGATGCAT GGAAGGTGTA CTTTGATGAG 
ACAGCCCAAG AAATTGTGGA TGAATTCGCT ATGCGCTATG GCATCGAGTC CATTTATCAG 
GCCATGACGC ACTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT ACCAGCCGTG 
ATGAGCACCT TACTGGCCAA CATCAATGCC TATTATGCTC ACACAACTGC CTCCACTAAC 
GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGTA AAGAAAGATT TGTAAAACTG 
CTGGACCAGC TACACAACTC ACTGAGGATA GACCTCTCTA CATACAGGAA TAACTTTCCT 
GCTGGGAGCC CTGAGCGGCT TCAGGATTTA AAGTCTACAG TGGATTTGCT GACCAGCATT 
ACTTTCTTCA GGATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTGGTA 
AAGGACTGTG TGAAAGCCTG CTTGAACTCT ACATATGAAT ACATCTTCAA CAACTGCCAT 
GACCTCTACA GTCACCAGTA TCAGCTGCAG GAACAACCTC TAGAGGAACC AGGGCCCAGC 
ATTCGGAACT TGGATTTCTG GCCCAAACTT ATCACACTCA TTGTTTCGAT CATAGAGGAG 
GATAAGAATT CCTACACACC TGTTCTGAGC CAGTTTCCTC AGGAGTTGAA CGTAGGAAAA 
GTCAGTGCAG AAGTGATGTG GCATCTGTTT GCTCAAGACA TGAAATATGC ACTGGAGGAA 
CACGAGAAAG ACCGGCTGTG TAAGAGTGCT GACTACATGA ACCTGCACTT CAAGGTGAAG 
TGGCTCCACA ATGAATATGT GCGCGATCTG CCTGCCCTCC AGGGGCAAGT GCCTGAGTAC 
CCAGCGTGGT TTGAGCAGTT TGTGCTACAG TGGCTAGATG AGAATGAAGA TGTGTCACTG 
GAATTCCTTC GTGGGGCCTT GGAACGAGAT AAGAAGGATG GGTTCCAGCA GACATCAGAG 
CATGCCTTGT TTTCCTGTTC TGTGGTGGAT GTCTTTACAC AACTCAACCA GAGCTTTGAG 
ATCATCCGGA AGTTGGAATG TCCAGACCCC AACATCCTTG CCCACTACAT GAGAAGGTTT 
GCTAAGACCA TTGGGAAAGT GCTGATGCAG TATGCAGACA TCTTATCAAA GAACTTCCCA 
GCTTACTGCA CAAAGGAGAG ACTGCCCTGT ATCCTGATGA ACAACATGCA GCAACTGAGG 
GTCCAGCTGG AGAAAATGTT TGAGGCCATG GGAGGCAAAG AGCTGGACTC CGAAGCTGCA 
GACAGTCTGA AGGAGCTGCA GGTGAAACTG AATACAGTTC TGGATGAGCT TAGCATGGTG 
TTTGGAAACA GCTTCCAGGT GCGGATTGAT GAATGTGTTC GACAAATGGC TGACATCCTG 
GGCCAAGTTC GGGGCACAGG GAATGCATCC CCTAACGCCA GGGCCTCGGT GGCTCAGGAT 
GCAGATAGTG TGCTGCGACC TCTCATGGAC TTCCTGGATG GCAACCTCAC GCTGTTTGCC 
ACTGTGTGTG AGAAGACAGT TCTGAAGCGG GTCCTGAAGG AGCTCTGGCG GGTGGTGATG 
AACACCATGG AGCGGGTTAT TGTCCTGCCC CCACTCACTG ACCAGACGGG CACCCAGCTG 
ATCTTAACTG CTGCCAAGGA GCTGAGCCAG CTTTCCAAAC TCAAGGACCA CATGGTACGG 
GAGGAAACAC GGAATCTCAC TCCAAAGCAG TGTGCTGTCC TCGACTTGGC CTTGGACACC 
ATCAAGCAAT ACTTCCATGC AGGAGGCAAT GGTCTGAAGA AAACCTTCCT GGAAAAGAGC 
CCAGACCTGC AGTCTCTGCG TTATGCCCTC TCTCTGTATA CACAGACTAC AGACACTCTC 
ATCAAGACAT TTGTGCGCTC ACAGACTGCC CAGGTACATG ATGGGAAAGG TATTAGGTTT 
ACTGCTAACG AGGACATTCG GCCAGAAAAG GGGGCTGGTG TGGATGACCC CGTGGGAGAA 
GTCTCTATTC AGGTGGACTT ATTTACACAT CCTGGCACTG GGGAGCATAA GGTCACAGTG 
AAAGTGGTAG CAGCCAATGA CCTCAAGTGG CAGACAGCGG GTATGTTCCG GCCTTTTGTG 
GAAGTGACCA TGGTTGGCCC ACACCAAAGT GATAAGAAGA GGAAGTTCAC AACCAAGTCT 
AAAAGTAACA ACTGGACTCC CAAGTACAAC GAGACGTTTC ACTTCCTCCT GGGAAATGAA 
GAGGGTCCAG AGGCCTATGA ATTGCAGATC TGTGTGAAGG ATTACTGCTT TGCCCGGGAG 
GATCGTGTGA TAGGACTGGC AGTGATGCCC CTGAGGGATG TGGCAGCTAA GGGCAGCTGT 
GCCTGCTGGT GCCCACTGGG CCGGAAGATC CATATGGATG AGACAGGCAT GACCATTCTC 
CGGATTCTGT CTCAGAGGAG CAATGACGAG GTAGCCCGAG AGTTCGTGAA GCTCAAATCA 
GAGTCTCGAT CCACAGAGGA AGGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537858.1
CDS	449..5278
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537795.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537795.4

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTCCTT TGAAGACCCT 
GATAGTGCCG TTGATGACCG AGACAGTGAC TACCGCAGTG AGACCAGCAA TAGCGCCCCA 
CCTCCTTACC ACACGACTAC CCAGCCCAAC GCTTCTGTAC ACCAGTTCCC TGTTCCTGTG 
CGGCTGCCAC AGCAGCTCTT TCTTCAAGGC AGTTCCCATG ACTCTTGCAA TGACTCTATG 
CAGAGTTACG ACCTTGATTA TCCAGAGCGT CGGGCCCTCA GCCCTACCAG CAGTAGTAGG 
TATGGCTCCT CCTGTAATGT GAGTCAAGGA AGCTCTCTGC TGAGTGAGCT GGACCAGTAT 
CATGAGCAAG ATGATGATGG TCGGGAGAGG GACTCCATTC ATTCTAGTCA TAGCTATGGC 
AGCCTCTCCA AAGATGGCCA GGCTGGTTTG GGAGAACAAG AGAAAGCCTT GGAAGTGACA 
TGTGAATCAG AAAAAGAGAA AACAGGTGAA TCCAAGGAGA TGAGAGATGA TGCTACAATC 
CACCCTCCTT CAGATCTGGT GCTGCATAAG GACCATGTCC TAGGACCCCA GGAGAGTCTT 
CCTGAGGAGA CCGCATCATC GCCATTTACC CAGGCCAGAG CACACTGGTT CCGAGCGGTT 
ACCAAGGTCC GACTCCAACT GCAGGAGATT TCAGATGATG GTGACCCCTC CTTGCCTCAG 
TGGCTCCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGA 
AGGAAGAAGC CACTGCCACT TGTCAGTGAT CTGTCACTGG TCCAGTCACG GAAAGCTGGG 
ATTACATCTG CTATGGCTAC ACGTACCTCT CTCAAGGATG AAGAACTGAA ATCTCATGTG 
TATAAGAAAA CTCTGCAGGC CTTAATCTAC CCCATCTCGT GTACCACGCC CCACAACTTT 
GAGGTCTGGT CTGCCACTAC ACCTACCTAT TGCTATGAGT GTGAAGGCTT GCTCTGGGGC 
CTTGCCCGGC AAGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG 
GACCTGCTCA ATGCTGACTG CCTACAGCGG GCTGCTGAAA AGAGCTCTAA GCATGGAGCT 
GAGGACCGAA CTCAGAACAT TATCATGGCC ATGAAGGACC GTATGAAGAT CCGAGAACGG 
AATAAACCAG AGATCTTTGA GGTTATCCGG GATGTGTTCA CAGTGAGCAA AGTTGCGCAT 
GTGCAGCAGA TGAAAACAGT AAAGCAGAGT GTCCTGGATG GCACCTCCAA GTGGTCAGCC 
AAAATTACCA TCACGGTGGT TTGTGCCCAG GGCCTACAAG CCAAAGACAA GACAGGATCC 
AGTGACCCTT ATGTGACTGT GCAAGTTGGG AAAACCAAGA AGCGCACCAA GACCATTTTT 
GGAAATTTGA ATCCTGTTTG GGAAGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAT 
CGAATTAAGG TACGCGTGTG GGATGAGGAT GATGACATCA AGTCCAGAGT AAAGCAGCGG 
CTAAAGCGAG AGTCAGATGA TTTCCTTGGC CAAACTATCA TTGAAGTTCG AACGCTGAGT 
GGAGAGATGG ATGTCTGGTA CAATCTGGAG AAGAGAACAG ACAAGTCAGC TGTCTCAGGG 
GCTATCCGAC TTCAAATCAG TGTGGAGATC AAGGGGGAGG AGAAGGTAGC CCCATACCAT 
GTACAATATA CATGTCTCCA TGAGAACCTT TTCCATTACC TCACAGACAT TCAGGGCAGT 
GGAGGAGTCT GGATCCCAGA GGCTCGGGGG GACGATGCAT GGAAGGTGTA CTTTGATGAG 
ACAGCCCAAG AAATTGTGGA TGAATTCGCT ATGCGCTATG GCATCGAGTC CATTTATCAG 
GCCATGACGC ACTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT ACCAGCCGTG 
ATGAGCACCT TACTGGCCAA CATCAATGCC TATTATGCTC ACACAACTGC CTCCACTAAC 
GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGTA AAGAAAGATT TGTAAAACTG 
CTGGACCAGC TACACAACTC ACTGAGGATA GACCTCTCTA CATACAGGAA TAACTTTCCT 
GCTGGGAGCC CTGAGCGGCT TCAGGATTTA AAGTCTACAG TGGATTTGCT GACCAGCATT 
ACTTTCTTCA GGATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTGGTA 
AAGGACTGTG TGAAAGCCTG CTTGAACTCT ACATATGAAT ACATCTTCAA CAACTGCCAT 
GACCTCTACA GTCACCAGTA TCAGCTGCAG GAACAACCTC TAGAGGAACC AGGGCCCAGC 
ATTCGGAACT TGGATTTCTG GCCCAAACTT ATCACACTCA TTGTTTCGAT CATAGAGGAG 
GATAAGAATT CCTACACACC TGTTCTGAGC CAGTTTCCTC AGGAGTTGAA CGTAGGAAAA 
GTCAGTGCAG AAGTGATGTG GCATCTGTTT GCTCAAGACA TGAAATATGC ACTGGAGGAA 
CACGAGAAAG ACCGGCTGTG TAAGAGTGCT GACTACATGA ACCTGCACTT CAAGGTGAAG 
TGGCTCCACA ATGAATATGT GCGCGATCTG CCTGCCCTCC AGGGGCAAGT GCCTGAGTAC 
CCAGCGTGGT TTGAGCAGTT TGTGCTACAG TGGCTAGATG AGAATGAAGA TGTGTCACTG 
GAATTCCTTC GTGGGGCCTT GGAACGAGAT AAGAAGGATG GGTTCCAGCA GACATCAGAG 
CATGCCTTGT TTTCCTGTTC TGTGGTGGAT GTCTTTACAC AACTCAACCA GAGCTTTGAG 
ATCATCCGGA AGTTGGAATG TCCAGACCCC AACATCCTTG CCCACTACAT GAGAAGGTTT 
GCTAAGACCA TTGGGAAAGT GCTGATGCAG TATGCAGACA TCTTATCAAA GAACTTCCCA 
GCTTACTGCA CAAAGGAGAG ACTGCCCTGT ATCCTGATGA ACAACATGCA GCAACTGAGG 
GTCCAGCTGG AGAAAATGTT TGAGGCCATG GGAGGCAAAG AGCTGGACTC CGAAGCTGCA 
GACAGTCTGA AGGAGCTGCA GGTGAAACTG AATACAGTTC TGGATGAGCT TAGCATGGTG 
TTTGGAAACA GCTTCCAGGT GCGGATTGAT GAATGTGTTC GACAAATGGC TGACATCCTG 
GGCCAAGTTC GGGGCACAGG GAATGCATCC CCTAACGCCA GGGCCTCGGT GGCTCAGGAT 
GCAGATAGTG TGCTGCGACC TCTCATGGAC TTCCTGGATG GCAACCTCAC GCTGTTTGCC 
ACTGTGTGTG AGAAGACAGT TCTGAAGCGG GTCCTGAAGG AGCTCTGGCG GGTGGTGATG 
AACACCATGG AGCGGGTTAT TGTCCTGCCC CCACTCACTG ACCAGACGGG CACCCAGCTG 
ATCTTAACTG CTGCCAAGGA GCTGAGCCAG CTTTCCAAAC TCAAGGACCA CATGGTACGG 
GAGGAAACAC GGAATCTCAC TCCAAAGCAG TGTGCTGTCC TCGACTTGGC CTTGGACACC 
ATCAAGCAAT ACTTCCATGC AGGAGGCAAT GGTCTGAAGA AAACCTTCCT GGAAAAGAGC 
CCAGACCTGC AGTCTCTGCG TTATGCCCTC TCTCTGTATA CACAGACTAC AGACACTCTC 
ATCAAGACAT TTGTGCGCTC ACAGACTGCC CAGGTACATG ATGGGAAAGG TATTAGGTTT 
ACTGCTAACG AGGACATTCG GCCAGAAAAG GGGGCTGGTG TGGATGACCC CGTGGGAGAA 
GTCTCTATTC AGGTGGACTT ATTTACACAT CCTGGCACTG GGGAGCATAA GGTCACAGTG 
AAAGTGGTAG CAGCCAATGA CCTCAAGTGG CAGACAGCGG GTATGTTCCG GCCTTTTGTG 
GAAGTGACCA TGGTTGGCCC ACACCAAAGT GATAAGAAGA GGAAGTTCAC AACCAAGTCT 
AAAAGTAACA ACTGGACTCC CAAGTACAAC GAGACGTTTC ACTTCCTCCT GGGAAATGAA 
GAGGGTCCAG AGGCCTATGA ATTGCAGATC TGTGTGAAGG ATTACTGCTT TGCCCGGGAG 
GATCGTGTGA TAGGACTGGC AGTGATGCCC CTGAGGGATG TGGCAGCTAA GGGCAGCTGT 
GCCTGCTGGT GCCCACTGGG CCGGAAGATC CATATGGATG AGACAGGCAT GACCATTCTC 
CGGATTCTGT CTCAGAGGAG CAATGACGAG GTAGCCCGAG AGTTCGTGAA GCTCAAATCA 
GAGTCTCGAT CCACAGAGGA AGGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_017320116.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175605.1 Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X7, mRNA.

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CloneID	OMu80937
Clone ID Related Accession (Same CDS sequence)	XM_017320116.1 , XM_017320116.2
Accession Version	XM_017320116.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGAGAC 
AGTGACTACC GCAGTGAGAC CAGCAATAGC GCCCCACCTC CTTACCACAC GACTACCCAG 
CCCAACGCTT CTGTACACCA GTTCCCTGTT CCTGTGCGGC TGCCACAGCA GCTCTTTCTT 
CAAGGCAGTT CCCATGACTC TTGCAATGAC TCTATGCAGA GTTACGACCT TGATTATCCA 
GAGCGTCGGG CCCTCAGCCC TACCAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CAAGGAAGCT CTCTGCTGAG TGAGCTGGAC CAGTATCATG AGCAAGATGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGTCATAGC TATGGCAGCC TCTCCAAAGA TGGCCAGGCT 
GGTTTGGGAG AACAAGAGAA AGCCTTGGAA GTGACATGTG AATCAGAAAA AGAGAAAACA 
GGTGAATCCA AGGAGATGAG AGATGATGCT ACAATCCACC CTCCTTCAGA TCTGGTGCTG 
CATAAGGACC ATGTCCTAGG ACCCCAGGAG AGTCTTCCTG AGGAGACCGC ATCATCGCCA 
TTTACCCAGG CCAGAGCACA CTGGTTCCGA GCGGTTACCA AGGTCCGACT CCAACTGCAG 
GAGATTTCAG ATGATGGTGA CCCCTCCTTG CCTCAGTGGC TCCCAGAAGG GCCAGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGAAGGA AGAAGCCACT GCCACTTGTC 
AGTGATCTGT CACTGGTCCA GTCACGGAAA GCTGGGATTA CATCTGCTAT GGCTACACGT 
ACCTCTCTCA AGGATGAAGA ACTGAAATCT CATGTGTATA AGAAAACTCT GCAGGCCTTA 
ATCTACCCCA TCTCGTGTAC CACGCCCCAC AACTTTGAGG TCTGGTCTGC CACTACACCT 
ACCTATTGCT ATGAGTGTGA AGGCTTGCTC TGGGGCCTTG CCCGGCAAGG CATGCGCTGC 
AGCGAGTGTG GAGTCAAGTG CCATGAGAAG TGCCAGGACC TGCTCAATGC TGACTGCCTA 
CAGCGGGCTG CTGAAAAGAG CTCTAAGCAT GGAGCTGAGG ACCGAACTCA GAACATTATC 
ATGGCCATGA AGGACCGTAT GAAGATCCGA GAACGGAATA AACCAGAGAT CTTTGAGGTT 
ATCCGGGATG TGTTCACAGT GAGCAAAGTT GCGCATGTGC AGCAGATGAA AACAGTAAAG 
CAGAGTGTCC TGGATGGCAC CTCCAAGTGG TCAGCCAAAA TTACCATCAC GGTGGTTTGT 
GCCCAGGGCC TACAAGCCAA AGACAAGACA GGATCCAGTG ACCCTTATGT GACTGTGCAA 
GTTGGGAAAA CCAAGAAGCG CACCAAGACC ATTTTTGGAA ATTTGAATCC TGTTTGGGAA 
GAGAAGTTCC ATTTTGAGTG CCACAACTCT TCTGATCGAA TTAAGGTACG CGTGTGGGAT 
GAGGATGATG ACATCAAGTC CAGAGTAAAG CAGCGGCTAA AGCGAGAGTC AGATGATTTC 
CTTGGCCAAA CTATCATTGA AGTTCGAACG CTGAGTGGAG AGATGGATGT CTGGTACAAT 
CTGGAGAAGA GAACAGACAA GTCAGCTGTC TCAGGGGCTA TCCGACTTCA AATCAGTGTG 
GAGATCAAGG GGGAGGAGAA GGTAGCCCCA TACCATGTAC AATATACATG TCTCCATGAG 
AACCTTTTCC ATTACCTCAC AGACATTCAG GGCAGTGGAG GAGTCTGGAT CCCAGAGGCT 
CGGGGGGACG ATGCATGGAA GGTGTACTTT GATGAGACAG CCCAAGAAAT TGTGGATGAA 
TTCGCTATGC GCTATGGCAT CGAGTCCATT TATCAGGCCA TGACGCACTT TGCATGTTTG 
TCATCCAAGT ACATGTGTCC TGGTGTACCA GCCGTGATGA GCACCTTACT GGCCAACATC 
AATGCCTATT ATGCTCACAC AACTGCCTCC ACTAACGTCT CTGCATCTGA TCGTTTTGCA 
GCCTCCAACT TTGGTAAAGA AAGATTTGTA AAACTGCTGG ACCAGCTACA CAACTCACTG 
AGGATAGACC TCTCTACATA CAGGAATAAC TTTCCTGCTG GGAGCCCTGA GCGGCTTCAG 
GATTTAAAGT CTACAGTGGA TTTGCTGACC AGCATTACTT TCTTCAGGAT GAAGGTGCAA 
GAACTTCAGA GCCCTCCAAG AGCCAGCCAG GTGGTAAAGG ACTGTGTGAA AGCCTGCTTG 
AACTCTACAT ATGAATACAT CTTCAACAAC TGCCATGACC TCTACAGTCA CCAGTATCAG 
CTGCAGGAAC AACCTCTAGA GGAACCAGGG CCCAGCATTC GGAACTTGGA TTTCTGGCCC 
AAACTTATCA CACTCATTGT TTCGATCATA GAGGAGGATA AGAATTCCTA CACACCTGTT 
CTGAGCCAGT TTCCTCAGGA GTTGAACGTA GGAAAAGTCA GTGCAGAAGT GATGTGGCAT 
CTGTTTGCTC AAGACATGAA ATATGCACTG GAGGAACACG AGAAAGACCG GCTGTGTAAG 
AGTGCTGACT ACATGAACCT GCACTTCAAG GTGAAGTGGC TCCACAATGA ATATGTGCGC 
GATCTGCCTG CCCTCCAGGG GCAAGTGCCT GAGTACCCAG CGTGGTTTGA GCAGTTTGTG 
CTACAGTGGC TAGATGAGAA TGAAGATGTG TCACTGGAAT TCCTTCGTGG GGCCTTGGAA 
CGAGATAAGA AGGATGGGTT CCAGCAGACA TCAGAGCATG CCTTGTTTTC CTGTTCTGTG 
GTGGATGTCT TTACACAACT CAACCAGAGC TTTGAGATCA TCCGGAAGTT GGAATGTCCA 
GACCCCAACA TCCTTGCCCA CTACATGAGA AGGTTTGCTA AGACCATTGG GAAAGTGCTG 
ATGCAGTATG CAGACATCTT ATCAAAGAAC TTCCCAGCTT ACTGCACAAA GGAGAGACTG 
CCCTGTATCC TGATGAACAA CATGCAGCAA CTGAGGGTCC AGCTGGAGAA AATGTTTGAG 
GCCATGGGAG GCAAAGAGCT GGACTCCGAA GCTGCAGACA GTCTGAAGGA GCTGCAGGTG 
AAACTGAATA CAGTTCTGGA TGAGCTTAGC ATGGTGTTTG GAAACAGCTT CCAGGTGCGG 
ATTGATGAAT GTGTTCGACA AATGGCTGAC ATCCTGGGCC AAGTTCGGGG CACAGGGAAT 
GCATCCCCTA ACGCCAGGGC CTCGGTGGCT CAGGATGCAG ATAGTGTGCT GCGACCTCTC 
ATGGACTTCC TGGATGGCAA CCTCACGCTG TTTGCCACTG TGTGTGAGAA GACAGTTCTG 
AAGCGGGTCC TGAAGGAGCT CTGGCGGGTG GTGATGAACA CCATGGAGCG GGTTATTGTC 
CTGCCCCCAC TCACTGACCA GACGGGCACC CAGCTGATCT TAACTGCTGC CAAGGAGCTG 
AGCCAGCTTT CCAAACTCAA GGACCACATG GTACGGGAGG AAACACGGAA TCTCACTCCA 
AAGCAGTGTG CTGTCCTCGA CTTGGCCTTG GACACCATCA AGCAATACTT CCATGCAGGA 
GGCAATGGTC TGAAGAAAAC CTTCCTGGAA AAGAGCCCAG ACCTGCAGTC TCTGCGTTAT 
GCCCTCTCTC TGTATACACA GACTACAGAC ACTCTCATCA AGACATTTGT GCGCTCACAG 
ACTGCCCAGG TACATGATGG GAAAGGTATT AGGTTTACTG CTAACGAGGA CATTCGGCCA 
GAAAAGGGGG CTGGTGTGGA TGACCCCGTG GGAGAAGTCT CTATTCAGGT GGACTTATTT 
ACACATCCTG GCACTGGGGA GCATAAGGTC ACAGTGAAAG TGGTAGCAGC CAATGACCTC 
AAGTGGCAGA CAGCGGGTAT GTTCCGGCCT TTTGTGGAAG TGACCATGGT TGGCCCACAC 
CAAAGTGATA AGAAGAGGAA GTTCACAACC AAGTCTAAAA GTAACAACTG GACTCCCAAG 
TACAACGAGA CGTTTCACTT CCTCCTGGGA AATGAAGAGG GTCCAGAGGC CTATGAATTG 
CAGATCTGTG TGAAGGATTA CTGCTTTGCC CGGGAGGATC GTGTGATAGG ACTGGCAGTG 
ATGCCCCTGA GGGATGTGGC AGCTAAGGGC AGCTGTGCCT GCTGGTGCCC ACTGGGCCGG 
AAGATCCATA TGGATGAGAC AGGCATGACC ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT 
GACGAGGTAG CCCGAGAGTT CGTGAAGCTC AAATCAGAGT CTCGATCCAC AGAGGAAGGG 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175605.1
CDS	336..5201
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCCHWTYLGWGEHQTFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_017320116.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320116.1

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGAGAC 
AGTGACTACC GCAGTGAGAC CAGCAATAGC GCCCCACCTC CTTACCACAC GACTACCCAG 
CCCAACGCTT CTGTACACCA GTTCCCTGTT CCTGTGCGGC TGCCACAGCA GCTCTTTCTT 
CAAGGCAGTT CCCATGACTC TTGCAATGAC TCTATGCAGA GTTACGACCT TGATTATCCA 
GAGCGTCGGG CCCTCAGCCC TACCAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CAAGGAAGCT CTCTGCTGAG TGAGCTGGAC CAGTATCATG AGCAAGATGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGTCATAGC TATGGCAGCC TCTCCAAAGA TGGCCAGGCT 
GGTTTGGGAG AACAAGAGAA AGCCTTGGAA GTGACATGTG AATCAGAAAA AGAGAAAACA 
GGTGAATCCA AGGAGATGAG AGATGATGCT ACAATCCACC CTCCTTCAGA TCTGGTGCTG 
CATAAGGACC ATGTCCTAGG ACCCCAGGAG AGTCTTCCTG AGGAGACCGC ATCATCGCCA 
TTTACCCAGG CCAGAGCACA CTGGTTCCGA GCGGTTACCA AGGTCCGACT CCAACTGCAG 
GAGATTTCAG ATGATGGTGA CCCCTCCTTG CCTCAGTGGC TCCCAGAAGG GCCAGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGAAGGA AGAAGCCACT GCCACTTGTC 
AGTGATCTGT CACTGGTCCA GTCACGGAAA GCTGGGATTA CATCTGCTAT GGCTACACGT 
ACCTCTCTCA AGGATGAAGA ACTGAAATCT CATGTGTATA AGAAAACTCT GCAGGCCTTA 
ATCTACCCCA TCTCGTGTAC CACGCCCCAC AACTTTGAGG TCTGGTCTGC CACTACACCT 
ACCTATTGCT ATGAGTGTGA AGGCTTGCTC TGGGGCCTTG CCCGGCAAGG CATGCGCTGC 
AGCGAGTGTG GAGTCAAGTG CCATGAGAAG TGCCAGGACC TGCTCAATGC TGACTGCCTA 
CAGCGGGCTG CTGAAAAGAG CTCTAAGCAT GGAGCTGAGG ACCGAACTCA GAACATTATC 
ATGGCCATGA AGGACCGTAT GAAGATCCGA GAACGGAATA AACCAGAGAT CTTTGAGGTT 
ATCCGGGATG TGTTCACAGT GAGCAAAGTT GCGCATGTGC AGCAGATGAA AACAGTAAAG 
CAGAGTGTCC TGGATGGCAC CTCCAAGTGG TCAGCCAAAA TTACCATCAC GGTGGTTTGT 
GCCCAGGGCC TACAAGCCAA AGACAAGACA GGATCCAGTG ACCCTTATGT GACTGTGCAA 
GTTGGGAAAA CCAAGAAGCG CACCAAGACC ATTTTTGGAA ATTTGAATCC TGTTTGGGAA 
GAGAAGTTCC ATTTTGAGTG CCACAACTCT TCTGATCGAA TTAAGGTACG CGTGTGGGAT 
GAGGATGATG ACATCAAGTC CAGAGTAAAG CAGCGGCTAA AGCGAGAGTC AGATGATTTC 
CTTGGCCAAA CTATCATTGA AGTTCGAACG CTGAGTGGAG AGATGGATGT CTGGTACAAT 
CTGGAGAAGA GAACAGACAA GTCAGCTGTC TCAGGGGCTA TCCGACTTCA AATCAGTGTG 
GAGATCAAGG GGGAGGAGAA GGTAGCCCCA TACCATGTAC AATATACATG TCTCCATGAG 
AACCTTTTCC ATTACCTCAC AGACATTCAG GGCAGTGGAG GAGTCTGGAT CCCAGAGGCT 
CGGGGGGACG ATGCATGGAA GGTGTACTTT GATGAGACAG CCCAAGAAAT TGTGGATGAA 
TTCGCTATGC GCTATGGCAT CGAGTCCATT TATCAGGCCA TGACGCACTT TGCATGTTTG 
TCATCCAAGT ACATGTGTCC TGGTGTACCA GCCGTGATGA GCACCTTACT GGCCAACATC 
AATGCCTATT ATGCTCACAC AACTGCCTCC ACTAACGTCT CTGCATCTGA TCGTTTTGCA 
GCCTCCAACT TTGGTAAAGA AAGATTTGTA AAACTGCTGG ACCAGCTACA CAACTCACTG 
AGGATAGACC TCTCTACATA CAGGAATAAC TTTCCTGCTG GGAGCCCTGA GCGGCTTCAG 
GATTTAAAGT CTACAGTGGA TTTGCTGACC AGCATTACTT TCTTCAGGAT GAAGGTGCAA 
GAACTTCAGA GCCCTCCAAG AGCCAGCCAG GTGGTAAAGG ACTGTGTGAA AGCCTGCTTG 
AACTCTACAT ATGAATACAT CTTCAACAAC TGCCATGACC TCTACAGTCA CCAGTATCAG 
CTGCAGGAAC AACCTCTAGA GGAACCAGGG CCCAGCATTC GGAACTTGGA TTTCTGGCCC 
AAACTTATCA CACTCATTGT TTCGATCATA GAGGAGGATA AGAATTCCTA CACACCTGTT 
CTGAGCCAGT TTCCTCAGGA GTTGAACGTA GGAAAAGTCA GTGCAGAAGT GATGTGGCAT 
CTGTTTGCTC AAGACATGAA ATATGCACTG GAGGAACACG AGAAAGACCG GCTGTGTAAG 
AGTGCTGACT ACATGAACCT GCACTTCAAG GTGAAGTGGC TCCACAATGA ATATGTGCGC 
GATCTGCCTG CCCTCCAGGG GCAAGTGCCT GAGTACCCAG CGTGGTTTGA GCAGTTTGTG 
CTACAGTGGC TAGATGAGAA TGAAGATGTG TCACTGGAAT TCCTTCGTGG GGCCTTGGAA 
CGAGATAAGA AGGATGGGTT CCAGCAGACA TCAGAGCATG CCTTGTTTTC CTGTTCTGTG 
GTGGATGTCT TTACACAACT CAACCAGAGC TTTGAGATCA TCCGGAAGTT GGAATGTCCA 
GACCCCAACA TCCTTGCCCA CTACATGAGA AGGTTTGCTA AGACCATTGG GAAAGTGCTG 
ATGCAGTATG CAGACATCTT ATCAAAGAAC TTCCCAGCTT ACTGCACAAA GGAGAGACTG 
CCCTGTATCC TGATGAACAA CATGCAGCAA CTGAGGGTCC AGCTGGAGAA AATGTTTGAG 
GCCATGGGAG GCAAAGAGCT GGACTCCGAA GCTGCAGACA GTCTGAAGGA GCTGCAGGTG 
AAACTGAATA CAGTTCTGGA TGAGCTTAGC ATGGTGTTTG GAAACAGCTT CCAGGTGCGG 
ATTGATGAAT GTGTTCGACA AATGGCTGAC ATCCTGGGCC AAGTTCGGGG CACAGGGAAT 
GCATCCCCTA ACGCCAGGGC CTCGGTGGCT CAGGATGCAG ATAGTGTGCT GCGACCTCTC 
ATGGACTTCC TGGATGGCAA CCTCACGCTG TTTGCCACTG TGTGTGAGAA GACAGTTCTG 
AAGCGGGTCC TGAAGGAGCT CTGGCGGGTG GTGATGAACA CCATGGAGCG GGTTATTGTC 
CTGCCCCCAC TCACTGACCA GACGGGCACC CAGCTGATCT TAACTGCTGC CAAGGAGCTG 
AGCCAGCTTT CCAAACTCAA GGACCACATG GTACGGGAGG AAACACGGAA TCTCACTCCA 
AAGCAGTGTG CTGTCCTCGA CTTGGCCTTG GACACCATCA AGCAATACTT CCATGCAGGA 
GGCAATGGTC TGAAGAAAAC CTTCCTGGAA AAGAGCCCAG ACCTGCAGTC TCTGCGTTAT 
GCCCTCTCTC TGTATACACA GACTACAGAC ACTCTCATCA AGACATTTGT GCGCTCACAG 
ACTGCCCAGG TACATGATGG GAAAGGTATT AGGTTTACTG CTAACGAGGA CATTCGGCCA 
GAAAAGGGGG CTGGTGTGGA TGACCCCGTG GGAGAAGTCT CTATTCAGGT GGACTTATTT 
ACACATCCTG GCACTGGGGA GCATAAGGTC ACAGTGAAAG TGGTAGCAGC CAATGACCTC 
AAGTGGCAGA CAGCGGGTAT GTTCCGGCCT TTTGTGGAAG TGACCATGGT TGGCCCACAC 
CAAAGTGATA AGAAGAGGAA GTTCACAACC AAGTCTAAAA GTAACAACTG GACTCCCAAG 
TACAACGAGA CGTTTCACTT CCTCCTGGGA AATGAAGAGG GTCCAGAGGC CTATGAATTG 
CAGATCTGTG TGAAGGATTA CTGCTTTGCC CGGGAGGATC GTGTGATAGG ACTGGCAGTG 
ATGCCCCTGA GGGATGTGGC AGCTAAGGGC AGCTGTGCCT GCTGGTGCCC ACTGGGCCGG 
AAGATCCATA TGGATGAGAC AGGCATGACC ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT 
GACGAGGTAG CCCGAGAGTT CGTGAAGCTC AAATCAGAGT CTCGATCCAC AGAGGAAGGG 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_017320116.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175605.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu80937
Clone ID Related Accession (Same CDS sequence)	XM_017320116.1 , XM_017320116.2
Accession Version	XM_017320116.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320116.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGAGAC 
AGTGACTACC GCAGTGAGAC CAGCAATAGC GCCCCACCTC CTTACCACAC GACTACCCAG 
CCCAACGCTT CTGTACACCA GTTCCCTGTT CCTGTGCGGC TGCCACAGCA GCTCTTTCTT 
CAAGGCAGTT CCCATGACTC TTGCAATGAC TCTATGCAGA GTTACGACCT TGATTATCCA 
GAGCGTCGGG CCCTCAGCCC TACCAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CAAGGAAGCT CTCTGCTGAG TGAGCTGGAC CAGTATCATG AGCAAGATGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGTCATAGC TATGGCAGCC TCTCCAAAGA TGGCCAGGCT 
GGTTTGGGAG AACAAGAGAA AGCCTTGGAA GTGACATGTG AATCAGAAAA AGAGAAAACA 
GGTGAATCCA AGGAGATGAG AGATGATGCT ACAATCCACC CTCCTTCAGA TCTGGTGCTG 
CATAAGGACC ATGTCCTAGG ACCCCAGGAG AGTCTTCCTG AGGAGACCGC ATCATCGCCA 
TTTACCCAGG CCAGAGCACA CTGGTTCCGA GCGGTTACCA AGGTCCGACT CCAACTGCAG 
GAGATTTCAG ATGATGGTGA CCCCTCCTTG CCTCAGTGGC TCCCAGAAGG GCCAGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGAAGGA AGAAGCCACT GCCACTTGTC 
AGTGATCTGT CACTGGTCCA GTCACGGAAA GCTGGGATTA CATCTGCTAT GGCTACACGT 
ACCTCTCTCA AGGATGAAGA ACTGAAATCT CATGTGTATA AGAAAACTCT GCAGGCCTTA 
ATCTACCCCA TCTCGTGTAC CACGCCCCAC AACTTTGAGG TCTGGTCTGC CACTACACCT 
ACCTATTGCT ATGAGTGTGA AGGCTTGCTC TGGGGCCTTG CCCGGCAAGG CATGCGCTGC 
AGCGAGTGTG GAGTCAAGTG CCATGAGAAG TGCCAGGACC TGCTCAATGC TGACTGCCTA 
CAGCGGGCTG CTGAAAAGAG CTCTAAGCAT GGAGCTGAGG ACCGAACTCA GAACATTATC 
ATGGCCATGA AGGACCGTAT GAAGATCCGA GAACGGAATA AACCAGAGAT CTTTGAGGTT 
ATCCGGGATG TGTTCACAGT GAGCAAAGTT GCGCATGTGC AGCAGATGAA AACAGTAAAG 
CAGAGTGTCC TGGATGGCAC CTCCAAGTGG TCAGCCAAAA TTACCATCAC GGTGGTTTGT 
GCCCAGGGCC TACAAGCCAA AGACAAGACA GGATCCAGTG ACCCTTATGT GACTGTGCAA 
GTTGGGAAAA CCAAGAAGCG CACCAAGACC ATTTTTGGAA ATTTGAATCC TGTTTGGGAA 
GAGAAGTTCC ATTTTGAGTG CCACAACTCT TCTGATCGAA TTAAGGTACG CGTGTGGGAT 
GAGGATGATG ACATCAAGTC CAGAGTAAAG CAGCGGCTAA AGCGAGAGTC AGATGATTTC 
CTTGGCCAAA CTATCATTGA AGTTCGAACG CTGAGTGGAG AGATGGATGT CTGGTACAAT 
CTGGAGAAGA GAACAGACAA GTCAGCTGTC TCAGGGGCTA TCCGACTTCA AATCAGTGTG 
GAGATCAAGG GGGAGGAGAA GGTAGCCCCA TACCATGTAC AATATACATG TCTCCATGAG 
AACCTTTTCC ATTACCTCAC AGACATTCAG GGCAGTGGAG GAGTCTGGAT CCCAGAGGCT 
CGGGGGGACG ATGCATGGAA GGTGTACTTT GATGAGACAG CCCAAGAAAT TGTGGATGAA 
TTCGCTATGC GCTATGGCAT CGAGTCCATT TATCAGGCCA TGACGCACTT TGCATGTTTG 
TCATCCAAGT ACATGTGTCC TGGTGTACCA GCCGTGATGA GCACCTTACT GGCCAACATC 
AATGCCTATT ATGCTCACAC AACTGCCTCC ACTAACGTCT CTGCATCTGA TCGTTTTGCA 
GCCTCCAACT TTGGTAAAGA AAGATTTGTA AAACTGCTGG ACCAGCTACA CAACTCACTG 
AGGATAGACC TCTCTACATA CAGGAATAAC TTTCCTGCTG GGAGCCCTGA GCGGCTTCAG 
GATTTAAAGT CTACAGTGGA TTTGCTGACC AGCATTACTT TCTTCAGGAT GAAGGTGCAA 
GAACTTCAGA GCCCTCCAAG AGCCAGCCAG GTGGTAAAGG ACTGTGTGAA AGCCTGCTTG 
AACTCTACAT ATGAATACAT CTTCAACAAC TGCCATGACC TCTACAGTCA CCAGTATCAG 
CTGCAGGAAC AACCTCTAGA GGAACCAGGG CCCAGCATTC GGAACTTGGA TTTCTGGCCC 
AAACTTATCA CACTCATTGT TTCGATCATA GAGGAGGATA AGAATTCCTA CACACCTGTT 
CTGAGCCAGT TTCCTCAGGA GTTGAACGTA GGAAAAGTCA GTGCAGAAGT GATGTGGCAT 
CTGTTTGCTC AAGACATGAA ATATGCACTG GAGGAACACG AGAAAGACCG GCTGTGTAAG 
AGTGCTGACT ACATGAACCT GCACTTCAAG GTGAAGTGGC TCCACAATGA ATATGTGCGC 
GATCTGCCTG CCCTCCAGGG GCAAGTGCCT GAGTACCCAG CGTGGTTTGA GCAGTTTGTG 
CTACAGTGGC TAGATGAGAA TGAAGATGTG TCACTGGAAT TCCTTCGTGG GGCCTTGGAA 
CGAGATAAGA AGGATGGGTT CCAGCAGACA TCAGAGCATG CCTTGTTTTC CTGTTCTGTG 
GTGGATGTCT TTACACAACT CAACCAGAGC TTTGAGATCA TCCGGAAGTT GGAATGTCCA 
GACCCCAACA TCCTTGCCCA CTACATGAGA AGGTTTGCTA AGACCATTGG GAAAGTGCTG 
ATGCAGTATG CAGACATCTT ATCAAAGAAC TTCCCAGCTT ACTGCACAAA GGAGAGACTG 
CCCTGTATCC TGATGAACAA CATGCAGCAA CTGAGGGTCC AGCTGGAGAA AATGTTTGAG 
GCCATGGGAG GCAAAGAGCT GGACTCCGAA GCTGCAGACA GTCTGAAGGA GCTGCAGGTG 
AAACTGAATA CAGTTCTGGA TGAGCTTAGC ATGGTGTTTG GAAACAGCTT CCAGGTGCGG 
ATTGATGAAT GTGTTCGACA AATGGCTGAC ATCCTGGGCC AAGTTCGGGG CACAGGGAAT 
GCATCCCCTA ACGCCAGGGC CTCGGTGGCT CAGGATGCAG ATAGTGTGCT GCGACCTCTC 
ATGGACTTCC TGGATGGCAA CCTCACGCTG TTTGCCACTG TGTGTGAGAA GACAGTTCTG 
AAGCGGGTCC TGAAGGAGCT CTGGCGGGTG GTGATGAACA CCATGGAGCG GGTTATTGTC 
CTGCCCCCAC TCACTGACCA GACGGGCACC CAGCTGATCT TAACTGCTGC CAAGGAGCTG 
AGCCAGCTTT CCAAACTCAA GGACCACATG GTACGGGAGG AAACACGGAA TCTCACTCCA 
AAGCAGTGTG CTGTCCTCGA CTTGGCCTTG GACACCATCA AGCAATACTT CCATGCAGGA 
GGCAATGGTC TGAAGAAAAC CTTCCTGGAA AAGAGCCCAG ACCTGCAGTC TCTGCGTTAT 
GCCCTCTCTC TGTATACACA GACTACAGAC ACTCTCATCA AGACATTTGT GCGCTCACAG 
ACTGCCCAGG TACATGATGG GAAAGGTATT AGGTTTACTG CTAACGAGGA CATTCGGCCA 
GAAAAGGGGG CTGGTGTGGA TGACCCCGTG GGAGAAGTCT CTATTCAGGT GGACTTATTT 
ACACATCCTG GCACTGGGGA GCATAAGGTC ACAGTGAAAG TGGTAGCAGC CAATGACCTC 
AAGTGGCAGA CAGCGGGTAT GTTCCGGCCT TTTGTGGAAG TGACCATGGT TGGCCCACAC 
CAAAGTGATA AGAAGAGGAA GTTCACAACC AAGTCTAAAA GTAACAACTG GACTCCCAAG 
TACAACGAGA CGTTTCACTT CCTCCTGGGA AATGAAGAGG GTCCAGAGGC CTATGAATTG 
CAGATCTGTG TGAAGGATTA CTGCTTTGCC CGGGAGGATC GTGTGATAGG ACTGGCAGTG 
ATGCCCCTGA GGGATGTGGC AGCTAAGGGC AGCTGTGCCT GCTGGTGCCC ACTGGGCCGG 
AAGATCCATA TGGATGAGAC AGGCATGACC ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT 
GACGAGGTAG CCCGAGAGTT CGTGAAGCTC AAATCAGAGT CTCGATCCAC AGAGGAAGGG 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175605.1
CDS	449..5314
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCCHWTYLGWGEHQTFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_017320116.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320116.2

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGAGAC 
AGTGACTACC GCAGTGAGAC CAGCAATAGC GCCCCACCTC CTTACCACAC GACTACCCAG 
CCCAACGCTT CTGTACACCA GTTCCCTGTT CCTGTGCGGC TGCCACAGCA GCTCTTTCTT 
CAAGGCAGTT CCCATGACTC TTGCAATGAC TCTATGCAGA GTTACGACCT TGATTATCCA 
GAGCGTCGGG CCCTCAGCCC TACCAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CAAGGAAGCT CTCTGCTGAG TGAGCTGGAC CAGTATCATG AGCAAGATGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGTCATAGC TATGGCAGCC TCTCCAAAGA TGGCCAGGCT 
GGTTTGGGAG AACAAGAGAA AGCCTTGGAA GTGACATGTG AATCAGAAAA AGAGAAAACA 
GGTGAATCCA AGGAGATGAG AGATGATGCT ACAATCCACC CTCCTTCAGA TCTGGTGCTG 
CATAAGGACC ATGTCCTAGG ACCCCAGGAG AGTCTTCCTG AGGAGACCGC ATCATCGCCA 
TTTACCCAGG CCAGAGCACA CTGGTTCCGA GCGGTTACCA AGGTCCGACT CCAACTGCAG 
GAGATTTCAG ATGATGGTGA CCCCTCCTTG CCTCAGTGGC TCCCAGAAGG GCCAGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGAAGGA AGAAGCCACT GCCACTTGTC 
AGTGATCTGT CACTGGTCCA GTCACGGAAA GCTGGGATTA CATCTGCTAT GGCTACACGT 
ACCTCTCTCA AGGATGAAGA ACTGAAATCT CATGTGTATA AGAAAACTCT GCAGGCCTTA 
ATCTACCCCA TCTCGTGTAC CACGCCCCAC AACTTTGAGG TCTGGTCTGC CACTACACCT 
ACCTATTGCT ATGAGTGTGA AGGCTTGCTC TGGGGCCTTG CCCGGCAAGG CATGCGCTGC 
AGCGAGTGTG GAGTCAAGTG CCATGAGAAG TGCCAGGACC TGCTCAATGC TGACTGCCTA 
CAGCGGGCTG CTGAAAAGAG CTCTAAGCAT GGAGCTGAGG ACCGAACTCA GAACATTATC 
ATGGCCATGA AGGACCGTAT GAAGATCCGA GAACGGAATA AACCAGAGAT CTTTGAGGTT 
ATCCGGGATG TGTTCACAGT GAGCAAAGTT GCGCATGTGC AGCAGATGAA AACAGTAAAG 
CAGAGTGTCC TGGATGGCAC CTCCAAGTGG TCAGCCAAAA TTACCATCAC GGTGGTTTGT 
GCCCAGGGCC TACAAGCCAA AGACAAGACA GGATCCAGTG ACCCTTATGT GACTGTGCAA 
GTTGGGAAAA CCAAGAAGCG CACCAAGACC ATTTTTGGAA ATTTGAATCC TGTTTGGGAA 
GAGAAGTTCC ATTTTGAGTG CCACAACTCT TCTGATCGAA TTAAGGTACG CGTGTGGGAT 
GAGGATGATG ACATCAAGTC CAGAGTAAAG CAGCGGCTAA AGCGAGAGTC AGATGATTTC 
CTTGGCCAAA CTATCATTGA AGTTCGAACG CTGAGTGGAG AGATGGATGT CTGGTACAAT 
CTGGAGAAGA GAACAGACAA GTCAGCTGTC TCAGGGGCTA TCCGACTTCA AATCAGTGTG 
GAGATCAAGG GGGAGGAGAA GGTAGCCCCA TACCATGTAC AATATACATG TCTCCATGAG 
AACCTTTTCC ATTACCTCAC AGACATTCAG GGCAGTGGAG GAGTCTGGAT CCCAGAGGCT 
CGGGGGGACG ATGCATGGAA GGTGTACTTT GATGAGACAG CCCAAGAAAT TGTGGATGAA 
TTCGCTATGC GCTATGGCAT CGAGTCCATT TATCAGGCCA TGACGCACTT TGCATGTTTG 
TCATCCAAGT ACATGTGTCC TGGTGTACCA GCCGTGATGA GCACCTTACT GGCCAACATC 
AATGCCTATT ATGCTCACAC AACTGCCTCC ACTAACGTCT CTGCATCTGA TCGTTTTGCA 
GCCTCCAACT TTGGTAAAGA AAGATTTGTA AAACTGCTGG ACCAGCTACA CAACTCACTG 
AGGATAGACC TCTCTACATA CAGGAATAAC TTTCCTGCTG GGAGCCCTGA GCGGCTTCAG 
GATTTAAAGT CTACAGTGGA TTTGCTGACC AGCATTACTT TCTTCAGGAT GAAGGTGCAA 
GAACTTCAGA GCCCTCCAAG AGCCAGCCAG GTGGTAAAGG ACTGTGTGAA AGCCTGCTTG 
AACTCTACAT ATGAATACAT CTTCAACAAC TGCCATGACC TCTACAGTCA CCAGTATCAG 
CTGCAGGAAC AACCTCTAGA GGAACCAGGG CCCAGCATTC GGAACTTGGA TTTCTGGCCC 
AAACTTATCA CACTCATTGT TTCGATCATA GAGGAGGATA AGAATTCCTA CACACCTGTT 
CTGAGCCAGT TTCCTCAGGA GTTGAACGTA GGAAAAGTCA GTGCAGAAGT GATGTGGCAT 
CTGTTTGCTC AAGACATGAA ATATGCACTG GAGGAACACG AGAAAGACCG GCTGTGTAAG 
AGTGCTGACT ACATGAACCT GCACTTCAAG GTGAAGTGGC TCCACAATGA ATATGTGCGC 
GATCTGCCTG CCCTCCAGGG GCAAGTGCCT GAGTACCCAG CGTGGTTTGA GCAGTTTGTG 
CTACAGTGGC TAGATGAGAA TGAAGATGTG TCACTGGAAT TCCTTCGTGG GGCCTTGGAA 
CGAGATAAGA AGGATGGGTT CCAGCAGACA TCAGAGCATG CCTTGTTTTC CTGTTCTGTG 
GTGGATGTCT TTACACAACT CAACCAGAGC TTTGAGATCA TCCGGAAGTT GGAATGTCCA 
GACCCCAACA TCCTTGCCCA CTACATGAGA AGGTTTGCTA AGACCATTGG GAAAGTGCTG 
ATGCAGTATG CAGACATCTT ATCAAAGAAC TTCCCAGCTT ACTGCACAAA GGAGAGACTG 
CCCTGTATCC TGATGAACAA CATGCAGCAA CTGAGGGTCC AGCTGGAGAA AATGTTTGAG 
GCCATGGGAG GCAAAGAGCT GGACTCCGAA GCTGCAGACA GTCTGAAGGA GCTGCAGGTG 
AAACTGAATA CAGTTCTGGA TGAGCTTAGC ATGGTGTTTG GAAACAGCTT CCAGGTGCGG 
ATTGATGAAT GTGTTCGACA AATGGCTGAC ATCCTGGGCC AAGTTCGGGG CACAGGGAAT 
GCATCCCCTA ACGCCAGGGC CTCGGTGGCT CAGGATGCAG ATAGTGTGCT GCGACCTCTC 
ATGGACTTCC TGGATGGCAA CCTCACGCTG TTTGCCACTG TGTGTGAGAA GACAGTTCTG 
AAGCGGGTCC TGAAGGAGCT CTGGCGGGTG GTGATGAACA CCATGGAGCG GGTTATTGTC 
CTGCCCCCAC TCACTGACCA GACGGGCACC CAGCTGATCT TAACTGCTGC CAAGGAGCTG 
AGCCAGCTTT CCAAACTCAA GGACCACATG GTACGGGAGG AAACACGGAA TCTCACTCCA 
AAGCAGTGTG CTGTCCTCGA CTTGGCCTTG GACACCATCA AGCAATACTT CCATGCAGGA 
GGCAATGGTC TGAAGAAAAC CTTCCTGGAA AAGAGCCCAG ACCTGCAGTC TCTGCGTTAT 
GCCCTCTCTC TGTATACACA GACTACAGAC ACTCTCATCA AGACATTTGT GCGCTCACAG 
ACTGCCCAGG TACATGATGG GAAAGGTATT AGGTTTACTG CTAACGAGGA CATTCGGCCA 
GAAAAGGGGG CTGGTGTGGA TGACCCCGTG GGAGAAGTCT CTATTCAGGT GGACTTATTT 
ACACATCCTG GCACTGGGGA GCATAAGGTC ACAGTGAAAG TGGTAGCAGC CAATGACCTC 
AAGTGGCAGA CAGCGGGTAT GTTCCGGCCT TTTGTGGAAG TGACCATGGT TGGCCCACAC 
CAAAGTGATA AGAAGAGGAA GTTCACAACC AAGTCTAAAA GTAACAACTG GACTCCCAAG 
TACAACGAGA CGTTTCACTT CCTCCTGGGA AATGAAGAGG GTCCAGAGGC CTATGAATTG 
CAGATCTGTG TGAAGGATTA CTGCTTTGCC CGGGAGGATC GTGTGATAGG ACTGGCAGTG 
ATGCCCCTGA GGGATGTGGC AGCTAAGGGC AGCTGTGCCT GCTGGTGCCC ACTGGGCCGG 
AAGATCCATA TGGATGAGAC AGGCATGACC ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT 
GACGAGGTAG CCCGAGAGTT CGTGAAGCTC AAATCAGAGT CTCGATCCAC AGAGGAAGGG 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_017320117.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175606.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant X10, mRNA.

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CloneID	OMu80938
Clone ID Related Accession (Same CDS sequence)	XM_017320117.1
Accession Version	XM_017320117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGCA CCGTGTGGAT TGCACTGAAG ACAATCCGTC AGTCGGATGA GGAAGGGCCC 
GGGGAGTGGT CTACACTGGA GGCAGAGACG TTAATGAAAG ATGATGAGAT CTGTGGAACT 
AAAAATCCAA CTCCTCATAA AATCTTGCTT GATACAAGAT TTGAGCTGCC TTTTGACATC 
CCAGAGGAGG AAGCCAGATA TTGGACCTAC AAATTGGAGC AAATCAATGC CTTGGCAGAT 
GATAATGAGT ATTCTAGTCA AGAAGAAAGC CAGAGGAAGC CATTACCCAC TGCTGCCGCC 
CAGTGTTGTC ACTGGACCTA TTTGGGCTGG GGAGAGCATC AGACCTTTGA AGACCCTGAT 
AGTGCCGTTG ATGACCGAGA CAGTGACTAC CGCAGTGAGA CCAGCAATAG CGCCCCACCT 
CCTTACCACA CGACTACCCA GCCCAACGCT TCTGTACACC AGTTCCCTGT TCCTGTGCGG 
CTGCCACAGC AGCTCTTTCT TCAAGGCAGT TCCCATGACT CTTGCAATGA CTCTATGCAG 
AGTTACGACC TTGATTATCC AGAGCGTCGG GCCCTCAGCC CTACCAGCAG TAGTAGGTAT 
GGCTCCTCCT GTAATGTGAG TCAAGGAAGC TCTCTGCTGA GTGAGCTGGA CCAGTATCAT 
GAGCAAGATG ATGATGGTCG GGAGAGGGAC TCCATTCATT CTAGTCATAG CTATGGCAGC 
CTCTCCAAAG ATGGCCAGGC TGGTTTGGGA GAACAAGAGA AAGCCTTGGA AGTGACATGT 
GAATCAGAAA AAGAGAAAAC AGGTGAATCC AAGGAGATGA GAGATGATGC TACAATCCAC 
CCTCCTTCAG ATCTGGTGCT GCATAAGGAC CATGTCCTAG GACCCCAGGA GAGTCTTCCT 
GAGGAGACCG CATCATCGCC ATTTACCCAG GCCAGAGCAC ACTGGTTCCG AGCGGTTACC 
AAGGTCCGAC TCCAACTGCA GGAGATTTCA GATGATGGTG ACCCCTCCTT GCCTCAGTGG 
CTCCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCAGA TCTACGAAGG 
AAGAAGCCAC TGCCACTTGT CAGTGATCTG GCTATGTCAC TGGTCCAGTC ACGGAAAGCT 
GGGATTACAT CTGCTATGGC TACACGTACC TCTCTCAAGG ATGAAGAACT GAAATCTCAT 
GTGTATAAGA AAACTCTGCA GGCCTTAATC TACCCCATCT CGTGTACCAC GCCCCACAAC 
TTTGAGGTCT GGTCTGCCAC TACACCTACC TATTGCTATG AGTGTGAAGG CTTGCTCTGG 
GGCCTTGCCC GGCAAGGCAT GCGCTGCAGC GAGTGTGGAG TCAAGTGCCA TGAGAAGTGC 
CAGGACCTGC TCAATGCTGA CTGCCTACAG CGGGCTGCTG AAAAGAGCTC TAAGCATGGA 
GCTGAGGACC GAACTCAGAA CATTATCATG GCCATGAAGG ACCGTATGAA GATCCGAGAA 
CGGAATAAAC CAGAGATCTT TGAGGTTATC CGGGATGTGT TCACAGTGAG CAAAGTTGCG 
CATGTGCAGC AGATGAAAAC AGTAAAGCAG AGTGTCCTGG ATGGCACCTC CAAGTGGTCA 
GCCAAAATTA CCATCACGGT GGTTTGTGCC CAGGGCCTAC AAGCCAAAGA CAAGACAGGA 
TCCAGTGACC CTTATGTGAC TGTGCAAGTT GGGAAAACCA AGAAGCGCAC CAAGACCATT 
TTTGGAAATT TGAATCCTGT TTGGGAAGAG AAGTTCCATT TTGAGTGCCA CAACTCTTCT 
GATCGAATTA AGGTACGCGT GTGGGATGAG GATGATGACA TCAAGTCCAG AGTAAAGCAG 
CGGCTAAAGC GAGAGTCAGA TGATTTCCTT GGCCAAACTA TCATTGAAGT TCGAACGCTG 
AGTGGAGAGA TGGATGTCTG GTACAATCTG GAGAAGAGAA CAGACAAGTC AGCTGTCTCA 
GGGGCTATCC GACTTCAAAT CAGTGTGGAG ATCAAGGGGG AGGAGAAGGT AGCCCCATAC 
CATGTACAAT ATACATGTCT CCATGAGAAC CTTTTCCATT ACCTCACAGA CATTCAGGGC 
AGTGGAGGAG TCTGGATCCC AGAGGCTCGG GGGGACGATG CATGGAAGGT GTACTTTGAT 
GAGACAGCCC AAGAAATTGT GGATGAATTC GCTATGCGCT ATGGCATCGA GTCCATTTAT 
CAGGCCATGA CGCACTTTGC ATGTTTGTCA TCCAAGTACA TGTGTCCTGG TGTACCAGCC 
GTGATGAGCA CCTTACTGGC CAACATCAAT GCCTATTATG CTCACACAAC TGCCTCCACT 
AACGTCTCTG CATCTGATCG TTTTGCAGCC TCCAACTTTG GTAAAGAAAG ATTTGTAAAA 
CTGCTGGACC AGCTACACAA CTCACTGAGG ATAGACCTCT CTACATACAG GAATAACTTT 
CCTGCTGGGA GCCCTGAGCG GCTTCAGGAT TTAAAGTCTA CAGTGGATTT GCTGACCAGC 
ATTACTTTCT TCAGGATGAA GGTGCAAGAA CTTCAGAGCC CTCCAAGAGC CAGCCAGGTG 
GTAAAGGACT GTGTGAAAGC CTGCTTGAAC TCTACATATG AATACATCTT CAACAACTGC 
CATGACCTCT ACAGTCACCA GTATCAGCTG CAGGAACAAC CTCTAGAGGA ACCAGGGCCC 
AGCATTCGGA ACTTGGATTT CTGGCCCAAA CTTATCACAC TCATTGTTTC GATCATAGAG 
GAGGATAAGA ATTCCTACAC ACCTGTTCTG AGCCAGTTTC CTCAGGAGTT GAACGTAGGA 
AAAGTCAGTG CAGAAGTGAT GTGGCATCTG TTTGCTCAAG ACATGAAATA TGCACTGGAG 
GAACACGAGA AAGACCGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG 
AAGTGGCTCC ACAATGAATA TGTGCGCGAT CTGCCTGCCC TCCAGGGGCA AGTGCCTGAG 
TACCCAGCGT GGTTTGAGCA GTTTGTGCTA CAGTGGCTAG ATGAGAATGA AGATGTGTCA 
CTGGAATTCC TTCGTGGGGC CTTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA 
GAGCATGCCT TGTTTTCCTG TTCTGTGGTG GATGTCTTTA CACAACTCAA CCAGAGCTTT 
GAGATCATCC GGAAGTTGGA ATGTCCAGAC CCCAACATCC TTGCCCACTA CATGAGAAGG 
TTTGCTAAGA CCATTGGGAA AGTGCTGATG CAGTATGCAG ACATCTTATC AAAGAACTTC 
CCAGCTTACT GCACAAAGGA GAGACTGCCC TGTATCCTGA TGAACAACAT GCAGCAACTG 
AGGGTCCAGC TGGAGAAAAT GTTTGAGGCC ATGGGAGGCA AAGAGCTGGA CTCCGAAGCT 
GCAGACAGTC TGAAGGAGCT GCAGGTGAAA CTGAATACAG TTCTGGATGA GCTTAGCATG 
GTGTTTGGAA ACAGCTTCCA GGTGCGGATT GATGAATGTG TTCGACAAAT GGCTGACATC 
CTGGGCCAAG TTCGGGGCAC AGGGAATGCA TCCCCTAACG CCAGGGCCTC GGTGGCTCAG 
GATGCAGATA GTGTGCTGCG ACCTCTCATG GACTTCCTGG ATGGCAACCT CACGCTGTTT 
GCCACTGTGT GTGAGAAGAC AGTTCTGAAG CGGGTCCTGA AGGAGCTCTG GCGGGTGGTG 
ATGAACACCA TGGAGCGGGT TATTGTCCTG CCCCCACTCA CTGACCAGAC GGGCACCCAG 
CTGATCTTAA CTGCTGCCAA GGAGCTGAGC CAGCTTTCCA AACTCAAGGA CCACATGGTA 
CGGGAGGAAA CACGGAATCT CACTCCAAAG CAGTGTGCTG TCCTCGACTT GGCCTTGGAC 
ACCATCAAGC AATACTTCCA TGCAGGAGGC AATGGTCTGA AGAAAACCTT CCTGGAAAAG 
AGCCCAGACC TGCAGTCTCT GCGTTATGCC CTCTCTCTGT ATACACAGAC TACAGACACT 
CTCATCAAGA CATTTGTGCG CTCACAGACT GCCCAGGTAC ATGATGGGAA AGGTATTAGG 
TTTACTGCTA ACGAGGACAT TCGGCCAGAA AAGGGGGCTG GTGTGGATGA CCCCGTGGGA 
GAAGTCTCTA TTCAGGTGGA CTTATTTACA CATCCTGGCA CTGGGGAGCA TAAGGTCACA 
GTGAAAGTGG TAGCAGCCAA TGACCTCAAG TGGCAGACAG CGGGTATGTT CCGGCCTTTT 
GTGGAAGTGA CCATGGTTGG CCCACACCAA AGTGATAAGA AGAGGAAGTT CACAACCAAG 
TCTAAAAGTA ACAACTGGAC TCCCAAGTAC AACGAGACGT TTCACTTCCT CCTGGGAAAT 
GAAGAGGGTC CAGAGGCCTA TGAATTGCAG ATCTGTGTGA AGGATTACTG CTTTGCCCGG 
GAGGATCGTG TGATAGGACT GGCAGTGATG CCCCTGAGGG ATGTGGCAGC TAAGGGCAGC 
TGTGCCTGCT GGTGCCCACT GGGCCGGAAG ATCCATATGG ATGAGACAGG CATGACCATT 
CTCCGGATTC TGTCTCAGAG GAGCAATGAC GAGGTAGCCC GAGAGTTCGT GAAGCTCAAA 
TCAGAGTCTC GATCCACAGA GGAAGGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175606.1
CDS	87..4739
Translation	MVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCCHWTYLGWGEHQTFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_017320117.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320117.1

ORF Insert Sequence:

ATGGTGGGCA CCGTGTGGAT TGCACTGAAG ACAATCCGTC AGTCGGATGA GGAAGGGCCC 
GGGGAGTGGT CTACACTGGA GGCAGAGACG TTAATGAAAG ATGATGAGAT CTGTGGAACT 
AAAAATCCAA CTCCTCATAA AATCTTGCTT GATACAAGAT TTGAGCTGCC TTTTGACATC 
CCAGAGGAGG AAGCCAGATA TTGGACCTAC AAATTGGAGC AAATCAATGC CTTGGCAGAT 
GATAATGAGT ATTCTAGTCA AGAAGAAAGC CAGAGGAAGC CATTACCCAC TGCTGCCGCC 
CAGTGTTGTC ACTGGACCTA TTTGGGCTGG GGAGAGCATC AGACCTTTGA AGACCCTGAT 
AGTGCCGTTG ATGACCGAGA CAGTGACTAC CGCAGTGAGA CCAGCAATAG CGCCCCACCT 
CCTTACCACA CGACTACCCA GCCCAACGCT TCTGTACACC AGTTCCCTGT TCCTGTGCGG 
CTGCCACAGC AGCTCTTTCT TCAAGGCAGT TCCCATGACT CTTGCAATGA CTCTATGCAG 
AGTTACGACC TTGATTATCC AGAGCGTCGG GCCCTCAGCC CTACCAGCAG TAGTAGGTAT 
GGCTCCTCCT GTAATGTGAG TCAAGGAAGC TCTCTGCTGA GTGAGCTGGA CCAGTATCAT 
GAGCAAGATG ATGATGGTCG GGAGAGGGAC TCCATTCATT CTAGTCATAG CTATGGCAGC 
CTCTCCAAAG ATGGCCAGGC TGGTTTGGGA GAACAAGAGA AAGCCTTGGA AGTGACATGT 
GAATCAGAAA AAGAGAAAAC AGGTGAATCC AAGGAGATGA GAGATGATGC TACAATCCAC 
CCTCCTTCAG ATCTGGTGCT GCATAAGGAC CATGTCCTAG GACCCCAGGA GAGTCTTCCT 
GAGGAGACCG CATCATCGCC ATTTACCCAG GCCAGAGCAC ACTGGTTCCG AGCGGTTACC 
AAGGTCCGAC TCCAACTGCA GGAGATTTCA GATGATGGTG ACCCCTCCTT GCCTCAGTGG 
CTCCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCAGA TCTACGAAGG 
AAGAAGCCAC TGCCACTTGT CAGTGATCTG GCTATGTCAC TGGTCCAGTC ACGGAAAGCT 
GGGATTACAT CTGCTATGGC TACACGTACC TCTCTCAAGG ATGAAGAACT GAAATCTCAT 
GTGTATAAGA AAACTCTGCA GGCCTTAATC TACCCCATCT CGTGTACCAC GCCCCACAAC 
TTTGAGGTCT GGTCTGCCAC TACACCTACC TATTGCTATG AGTGTGAAGG CTTGCTCTGG 
GGCCTTGCCC GGCAAGGCAT GCGCTGCAGC GAGTGTGGAG TCAAGTGCCA TGAGAAGTGC 
CAGGACCTGC TCAATGCTGA CTGCCTACAG CGGGCTGCTG AAAAGAGCTC TAAGCATGGA 
GCTGAGGACC GAACTCAGAA CATTATCATG GCCATGAAGG ACCGTATGAA GATCCGAGAA 
CGGAATAAAC CAGAGATCTT TGAGGTTATC CGGGATGTGT TCACAGTGAG CAAAGTTGCG 
CATGTGCAGC AGATGAAAAC AGTAAAGCAG AGTGTCCTGG ATGGCACCTC CAAGTGGTCA 
GCCAAAATTA CCATCACGGT GGTTTGTGCC CAGGGCCTAC AAGCCAAAGA CAAGACAGGA 
TCCAGTGACC CTTATGTGAC TGTGCAAGTT GGGAAAACCA AGAAGCGCAC CAAGACCATT 
TTTGGAAATT TGAATCCTGT TTGGGAAGAG AAGTTCCATT TTGAGTGCCA CAACTCTTCT 
GATCGAATTA AGGTACGCGT GTGGGATGAG GATGATGACA TCAAGTCCAG AGTAAAGCAG 
CGGCTAAAGC GAGAGTCAGA TGATTTCCTT GGCCAAACTA TCATTGAAGT TCGAACGCTG 
AGTGGAGAGA TGGATGTCTG GTACAATCTG GAGAAGAGAA CAGACAAGTC AGCTGTCTCA 
GGGGCTATCC GACTTCAAAT CAGTGTGGAG ATCAAGGGGG AGGAGAAGGT AGCCCCATAC 
CATGTACAAT ATACATGTCT CCATGAGAAC CTTTTCCATT ACCTCACAGA CATTCAGGGC 
AGTGGAGGAG TCTGGATCCC AGAGGCTCGG GGGGACGATG CATGGAAGGT GTACTTTGAT 
GAGACAGCCC AAGAAATTGT GGATGAATTC GCTATGCGCT ATGGCATCGA GTCCATTTAT 
CAGGCCATGA CGCACTTTGC ATGTTTGTCA TCCAAGTACA TGTGTCCTGG TGTACCAGCC 
GTGATGAGCA CCTTACTGGC CAACATCAAT GCCTATTATG CTCACACAAC TGCCTCCACT 
AACGTCTCTG CATCTGATCG TTTTGCAGCC TCCAACTTTG GTAAAGAAAG ATTTGTAAAA 
CTGCTGGACC AGCTACACAA CTCACTGAGG ATAGACCTCT CTACATACAG GAATAACTTT 
CCTGCTGGGA GCCCTGAGCG GCTTCAGGAT TTAAAGTCTA CAGTGGATTT GCTGACCAGC 
ATTACTTTCT TCAGGATGAA GGTGCAAGAA CTTCAGAGCC CTCCAAGAGC CAGCCAGGTG 
GTAAAGGACT GTGTGAAAGC CTGCTTGAAC TCTACATATG AATACATCTT CAACAACTGC 
CATGACCTCT ACAGTCACCA GTATCAGCTG CAGGAACAAC CTCTAGAGGA ACCAGGGCCC 
AGCATTCGGA ACTTGGATTT CTGGCCCAAA CTTATCACAC TCATTGTTTC GATCATAGAG 
GAGGATAAGA ATTCCTACAC ACCTGTTCTG AGCCAGTTTC CTCAGGAGTT GAACGTAGGA 
AAAGTCAGTG CAGAAGTGAT GTGGCATCTG TTTGCTCAAG ACATGAAATA TGCACTGGAG 
GAACACGAGA AAGACCGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG 
AAGTGGCTCC ACAATGAATA TGTGCGCGAT CTGCCTGCCC TCCAGGGGCA AGTGCCTGAG 
TACCCAGCGT GGTTTGAGCA GTTTGTGCTA CAGTGGCTAG ATGAGAATGA AGATGTGTCA 
CTGGAATTCC TTCGTGGGGC CTTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA 
GAGCATGCCT TGTTTTCCTG TTCTGTGGTG GATGTCTTTA CACAACTCAA CCAGAGCTTT 
GAGATCATCC GGAAGTTGGA ATGTCCAGAC CCCAACATCC TTGCCCACTA CATGAGAAGG 
TTTGCTAAGA CCATTGGGAA AGTGCTGATG CAGTATGCAG ACATCTTATC AAAGAACTTC 
CCAGCTTACT GCACAAAGGA GAGACTGCCC TGTATCCTGA TGAACAACAT GCAGCAACTG 
AGGGTCCAGC TGGAGAAAAT GTTTGAGGCC ATGGGAGGCA AAGAGCTGGA CTCCGAAGCT 
GCAGACAGTC TGAAGGAGCT GCAGGTGAAA CTGAATACAG TTCTGGATGA GCTTAGCATG 
GTGTTTGGAA ACAGCTTCCA GGTGCGGATT GATGAATGTG TTCGACAAAT GGCTGACATC 
CTGGGCCAAG TTCGGGGCAC AGGGAATGCA TCCCCTAACG CCAGGGCCTC GGTGGCTCAG 
GATGCAGATA GTGTGCTGCG ACCTCTCATG GACTTCCTGG ATGGCAACCT CACGCTGTTT 
GCCACTGTGT GTGAGAAGAC AGTTCTGAAG CGGGTCCTGA AGGAGCTCTG GCGGGTGGTG 
ATGAACACCA TGGAGCGGGT TATTGTCCTG CCCCCACTCA CTGACCAGAC GGGCACCCAG 
CTGATCTTAA CTGCTGCCAA GGAGCTGAGC CAGCTTTCCA AACTCAAGGA CCACATGGTA 
CGGGAGGAAA CACGGAATCT CACTCCAAAG CAGTGTGCTG TCCTCGACTT GGCCTTGGAC 
ACCATCAAGC AATACTTCCA TGCAGGAGGC AATGGTCTGA AGAAAACCTT CCTGGAAAAG 
AGCCCAGACC TGCAGTCTCT GCGTTATGCC CTCTCTCTGT ATACACAGAC TACAGACACT 
CTCATCAAGA CATTTGTGCG CTCACAGACT GCCCAGGTAC ATGATGGGAA AGGTATTAGG 
TTTACTGCTA ACGAGGACAT TCGGCCAGAA AAGGGGGCTG GTGTGGATGA CCCCGTGGGA 
GAAGTCTCTA TTCAGGTGGA CTTATTTACA CATCCTGGCA CTGGGGAGCA TAAGGTCACA 
GTGAAAGTGG TAGCAGCCAA TGACCTCAAG TGGCAGACAG CGGGTATGTT CCGGCCTTTT 
GTGGAAGTGA CCATGGTTGG CCCACACCAA AGTGATAAGA AGAGGAAGTT CACAACCAAG 
TCTAAAAGTA ACAACTGGAC TCCCAAGTAC AACGAGACGT TTCACTTCCT CCTGGGAAAT 
GAAGAGGGTC CAGAGGCCTA TGAATTGCAG ATCTGTGTGA AGGATTACTG CTTTGCCCGG 
GAGGATCGTG TGATAGGACT GGCAGTGATG CCCCTGAGGG ATGTGGCAGC TAAGGGCAGC 
TGTGCCTGCT GGTGCCCACT GGGCCGGAAG ATCCATATGG ATGAGACAGG CATGACCATT 
CTCCGGATTC TGTCTCAGAG GAGCAATGAC GAGGTAGCCC GAGAGTTCGT GAAGCTCAAA 
TCAGAGTCTC GATCCACAGA GGAAGGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_017320118.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175607.1 Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X11, mRNA.

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CloneID	OMu80939
Clone ID Related Accession (Same CDS sequence)	XM_017320118.1 , XM_030253406.1 , XM_017320118.2
Accession Version	XM_017320118.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAT CTGTGGAACT AAAAATCCAA CTCCTCATAA AATCTTGCTT GATACAAGAT 
TTGAGCTGCC TTTTGACATC CCAGAGGAGG AAGCCAGATA TTGGACCTAC AAATTGGAGC 
AAATCAATGC CTTGGCAGAT GATAATGAGT ATTCTAGTCA AGAAGAAAGC CAGAGGAAGC 
CATTACCCAC TGCTGCCGCC CAGTGTTGTC ACTGGACCTA TTTGGGCTGG GGAGAGCATC 
AGAGTACCCT TTGAAGACCC TGATAGTGCC GTTGATGACC GAGACAGTGA CTACCGCAGT 
GAGACCAGCA ATAGCGCCCC ACCTCCTTAC CACACGACTA CCCAGCCCAA CGCTTCTGTA 
CACCAGTTCC CTGTTCCTGT GCGGCTGCCA CAGCAGCTCT TTCTTCAAGG CAGTTCCCAT 
GACTCTTGCA ATGACTCTAT GCAGAGTTAC GACCTTGATT ATCCAGAGCG TCGGGCCCTC 
AGCCCTACCA GCAGTAGTAG GTATGGCTCC TCCTGTAATG TGAGTCAAGG AAGCTCTCTG 
CTGAGTGAGC TGGACCAGTA TCATGAGCAA GATGATGATG GTCGGGAGAG GGACTCCATT 
CATTCTAGTC ATAGCTATGG CAGCCTCTCC AAAGATGGCC AGGCTGGTTT GGGAGAACAA 
GAGAAAGCCT TGGAAGTGAC ATGTGAATCA GAAAAAGAGA AAACAGGTGA ATCCAAGGAG 
ATGAGAGATG ATGCTACAAT CCACCCTCCT TCAGATCTGG TGCTGCATAA GGACCATGTC 
CTAGGACCCC AGGAGAGTCT TCCTGAGGAG ACCGCATCAT CGCCATTTAC CCAGGCCAGA 
GCACACTGGT TCCGAGCGGT TACCAAGGTC CGACTCCAAC TGCAGGAGAT TTCAGATGAT 
GGTGACCCCT CCTTGCCTCA GTGGCTCCCA GAAGGGCCAG CTGGAGGGCT CTATGGCATT 
GACAGCATGC CAGATCTACG AAGGAAGAAG CCACTGCCAC TTGTCAGTGA TCTGGCTATG 
TCACTGGTCC AGTCACGGAA AGCTGGGATT ACATCTGCTA TGGCTACACG TACCTCTCTC 
AAGGATGAAG AACTGAAATC TCATGTGTAT AAGAAAACTC TGCAGGCCTT AATCTACCCC 
ATCTCGTGTA CCACGCCCCA CAACTTTGAG GTCTGGTCTG CCACTACACC TACCTATTGC 
TATGAGTGTG AAGGCTTGCT CTGGGGCCTT GCCCGGCAAG GCATGCGCTG CAGCGAGTGT 
GGAGTCAAGT GCCATGAGAA GTGCCAGGAC CTGCTCAATG CTGACTGCCT ACAGCGGGCT 
GCTGAAAAGA GCTCTAAGCA TGGAGCTGAG GACCGAACTC AGAACATTAT CATGGCCATG 
AAGGACCGTA TGAAGATCCG AGAACGGAAT AAACCAGAGA TCTTTGAGGT TATCCGGGAT 
GTGTTCACAG TGAGCAAAGT TGCGCATGTG CAGCAGATGA AAACAGTAAA GCAGAGTGTC 
CTGGATGGCA CCTCCAAGTG GTCAGCCAAA ATTACCATCA CGGTGGTTTG TGCCCAGGGC 
CTACAAGCCA AAGACAAGAC AGGATCCAGT GACCCTTATG TGACTGTGCA AGTTGGGAAA 
ACCAAGAAGC GCACCAAGAC CATTTTTGGA AATTTGAATC CTGTTTGGGA AGAGAAGTTC 
CATTTTGAGT GCCACAACTC TTCTGATCGA ATTAAGGTAC GCGTGTGGGA TGAGGATGAT 
GACATCAAGT CCAGAGTAAA GCAGCGGCTA AAGCGAGAGT CAGATGATTT CCTTGGCCAA 
ACTATCATTG AAGTTCGAAC GCTGAGTGGA GAGATGGATG TCTGGTACAA TCTGGAGAAG 
AGAACAGACA AGTCAGCTGT CTCAGGGGCT ATCCGACTTC AAATCAGTGT GGAGATCAAG 
GGGGAGGAGA AGGTAGCCCC ATACCATGTA CAATATACAT GTCTCCATGA GAACCTTTTC 
CATTACCTCA CAGACATTCA GGGCAGTGGA GGAGTCTGGA TCCCAGAGGC TCGGGGGGAC 
GATGCATGGA AGGTGTACTT TGATGAGACA GCCCAAGAAA TTGTGGATGA ATTCGCTATG 
CGCTATGGCA TCGAGTCCAT TTATCAGGCC ATGACGCACT TTGCATGTTT GTCATCCAAG 
TACATGTGTC CTGGTGTACC AGCCGTGATG AGCACCTTAC TGGCCAACAT CAATGCCTAT 
TATGCTCACA CAACTGCCTC CACTAACGTC TCTGCATCTG ATCGTTTTGC AGCCTCCAAC 
TTTGGTAAAG AAAGATTTGT AAAACTGCTG GACCAGCTAC ACAACTCACT GAGGATAGAC 
CTCTCTACAT ACAGGAATAA CTTTCCTGCT GGGAGCCCTG AGCGGCTTCA GGATTTAAAG 
TCTACAGTGG ATTTGCTGAC CAGCATTACT TTCTTCAGGA TGAAGGTGCA AGAACTTCAG 
AGCCCTCCAA GAGCCAGCCA GGTGGTAAAG GACTGTGTGA AAGCCTGCTT GAACTCTACA 
TATGAATACA TCTTCAACAA CTGCCATGAC CTCTACAGTC ACCAGTATCA GCTGCAGGAA 
CAACCTCTAG AGGAACCAGG GCCCAGCATT CGGAACTTGG ATTTCTGGCC CAAACTTATC 
ACACTCATTG TTTCGATCAT AGAGGAGGAT AAGAATTCCT ACACACCTGT TCTGAGCCAG 
TTTCCTCAGG AGTTGAACGT AGGAAAAGTC AGTGCAGAAG TGATGTGGCA TCTGTTTGCT 
CAAGACATGA AATATGCACT GGAGGAACAC GAGAAAGACC GGCTGTGTAA GAGTGCTGAC 
TACATGAACC TGCACTTCAA GGTGAAGTGG CTCCACAATG AATATGTGCG CGATCTGCCT 
GCCCTCCAGG GGCAAGTGCC TGAGTACCCA GCGTGGTTTG AGCAGTTTGT GCTACAGTGG 
CTAGATGAGA ATGAAGATGT GTCACTGGAA TTCCTTCGTG GGGCCTTGGA ACGAGATAAG 
AAGGATGGGT TCCAGCAGAC ATCAGAGCAT GCCTTGTTTT CCTGTTCTGT GGTGGATGTC 
TTTACACAAC TCAACCAGAG CTTTGAGATC ATCCGGAAGT TGGAATGTCC AGACCCCAAC 
ATCCTTGCCC ACTACATGAG AAGGTTTGCT AAGACCATTG GGAAAGTGCT GATGCAGTAT 
GCAGACATCT TATCAAAGAA CTTCCCAGCT TACTGCACAA AGGAGAGACT GCCCTGTATC 
CTGATGAACA ACATGCAGCA ACTGAGGGTC CAGCTGGAGA AAATGTTTGA GGCCATGGGA 
GGCAAAGAGC TGGACTCCGA AGCTGCAGAC AGTCTGAAGG AGCTGCAGGT GAAACTGAAT 
ACAGTTCTGG ATGAGCTTAG CATGGTGTTT GGAAACAGCT TCCAGGTGCG GATTGATGAA 
TGTGTTCGAC AAATGGCTGA CATCCTGGGC CAAGTTCGGG GCACAGGGAA TGCATCCCCT 
AACGCCAGGG CCTCGGTGGC TCAGGATGCA GATAGTGTGC TGCGACCTCT CATGGACTTC 
CTGGATGGCA ACCTCACGCT GTTTGCCACT GTGTGTGAGA AGACAGTTCT GAAGCGGGTC 
CTGAAGGAGC TCTGGCGGGT GGTGATGAAC ACCATGGAGC GGGTTATTGT CCTGCCCCCA 
CTCACTGACC AGACGGGCAC CCAGCTGATC TTAACTGCTG CCAAGGAGCT GAGCCAGCTT 
TCCAAACTCA AGGACCACAT GGTACGGGAG GAAACACGGA ATCTCACTCC AAAGCAGTGT 
GCTGTCCTCG ACTTGGCCTT GGACACCATC AAGCAATACT TCCATGCAGG AGGCAATGGT 
CTGAAGAAAA CCTTCCTGGA AAAGAGCCCA GACCTGCAGT CTCTGCGTTA TGCCCTCTCT 
CTGTATACAC AGACTACAGA CACTCTCATC AAGACATTTG TGCGCTCACA GACTGCCCAG 
GTACATGATG GGAAAGGTAT TAGGTTTACT GCTAACGAGG ACATTCGGCC AGAAAAGGGG 
GCTGGTGTGG ATGACCCCGT GGGAGAAGTC TCTATTCAGG TGGACTTATT TACACATCCT 
GGCACTGGGG AGCATAAGGT CACAGTGAAA GTGGTAGCAG CCAATGACCT CAAGTGGCAG 
ACAGCGGGTA TGTTCCGGCC TTTTGTGGAA GTGACCATGG TTGGCCCACA CCAAAGTGAT 
AAGAAGAGGA AGTTCACAAC CAAGTCTAAA AGTAACAACT GGACTCCCAA GTACAACGAG 
ACGTTTCACT TCCTCCTGGG AAATGAAGAG GGTCCAGAGG CCTATGAATT GCAGATCTGT 
GTGAAGGATT ACTGCTTTGC CCGGGAGGAT CGTGTGATAG GACTGGCAGT GATGCCCCTG 
AGGGATGTGG CAGCTAAGGG CAGCTGTGCC TGCTGGTGCC CACTGGGCCG GAAGATCCAT 
ATGGATGAGA CAGGCATGAC CATTCTCCGG ATTCTGTCTC AGAGGAGCAA TGACGAGGTA 
GCCCGAGAGT TCGTGAAGCT CAAATCAGAG TCTCGATCCA CAGAGGAAGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175607.1
CDS	655..5211
Translation	MMRSVELKIQLLIKSCLIQDLSCLLTSQRRKPDIGPTNWSKSMPWQMIMSILVKKKARGSHYPLLPPSVVTGPIWAGESIRVPFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_017320118.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320118.1

ORF Insert Sequence:

ATGATGAGAT CTGTGGAACT AAAAATCCAA CTCCTCATAA AATCTTGCTT GATACAAGAT 
TTGAGCTGCC TTTTGACATC CCAGAGGAGG AAGCCAGATA TTGGACCTAC AAATTGGAGC 
AAATCAATGC CTTGGCAGAT GATAATGAGT ATTCTAGTCA AGAAGAAAGC CAGAGGAAGC 
CATTACCCAC TGCTGCCGCC CAGTGTTGTC ACTGGACCTA TTTGGGCTGG GGAGAGCATC 
AGAGTACCCT TTGAAGACCC TGATAGTGCC GTTGATGACC GAGACAGTGA CTACCGCAGT 
GAGACCAGCA ATAGCGCCCC ACCTCCTTAC CACACGACTA CCCAGCCCAA CGCTTCTGTA 
CACCAGTTCC CTGTTCCTGT GCGGCTGCCA CAGCAGCTCT TTCTTCAAGG CAGTTCCCAT 
GACTCTTGCA ATGACTCTAT GCAGAGTTAC GACCTTGATT ATCCAGAGCG TCGGGCCCTC 
AGCCCTACCA GCAGTAGTAG GTATGGCTCC TCCTGTAATG TGAGTCAAGG AAGCTCTCTG 
CTGAGTGAGC TGGACCAGTA TCATGAGCAA GATGATGATG GTCGGGAGAG GGACTCCATT 
CATTCTAGTC ATAGCTATGG CAGCCTCTCC AAAGATGGCC AGGCTGGTTT GGGAGAACAA 
GAGAAAGCCT TGGAAGTGAC ATGTGAATCA GAAAAAGAGA AAACAGGTGA ATCCAAGGAG 
ATGAGAGATG ATGCTACAAT CCACCCTCCT TCAGATCTGG TGCTGCATAA GGACCATGTC 
CTAGGACCCC AGGAGAGTCT TCCTGAGGAG ACCGCATCAT CGCCATTTAC CCAGGCCAGA 
GCACACTGGT TCCGAGCGGT TACCAAGGTC CGACTCCAAC TGCAGGAGAT TTCAGATGAT 
GGTGACCCCT CCTTGCCTCA GTGGCTCCCA GAAGGGCCAG CTGGAGGGCT CTATGGCATT 
GACAGCATGC CAGATCTACG AAGGAAGAAG CCACTGCCAC TTGTCAGTGA TCTGGCTATG 
TCACTGGTCC AGTCACGGAA AGCTGGGATT ACATCTGCTA TGGCTACACG TACCTCTCTC 
AAGGATGAAG AACTGAAATC TCATGTGTAT AAGAAAACTC TGCAGGCCTT AATCTACCCC 
ATCTCGTGTA CCACGCCCCA CAACTTTGAG GTCTGGTCTG CCACTACACC TACCTATTGC 
TATGAGTGTG AAGGCTTGCT CTGGGGCCTT GCCCGGCAAG GCATGCGCTG CAGCGAGTGT 
GGAGTCAAGT GCCATGAGAA GTGCCAGGAC CTGCTCAATG CTGACTGCCT ACAGCGGGCT 
GCTGAAAAGA GCTCTAAGCA TGGAGCTGAG GACCGAACTC AGAACATTAT CATGGCCATG 
AAGGACCGTA TGAAGATCCG AGAACGGAAT AAACCAGAGA TCTTTGAGGT TATCCGGGAT 
GTGTTCACAG TGAGCAAAGT TGCGCATGTG CAGCAGATGA AAACAGTAAA GCAGAGTGTC 
CTGGATGGCA CCTCCAAGTG GTCAGCCAAA ATTACCATCA CGGTGGTTTG TGCCCAGGGC 
CTACAAGCCA AAGACAAGAC AGGATCCAGT GACCCTTATG TGACTGTGCA AGTTGGGAAA 
ACCAAGAAGC GCACCAAGAC CATTTTTGGA AATTTGAATC CTGTTTGGGA AGAGAAGTTC 
CATTTTGAGT GCCACAACTC TTCTGATCGA ATTAAGGTAC GCGTGTGGGA TGAGGATGAT 
GACATCAAGT CCAGAGTAAA GCAGCGGCTA AAGCGAGAGT CAGATGATTT CCTTGGCCAA 
ACTATCATTG AAGTTCGAAC GCTGAGTGGA GAGATGGATG TCTGGTACAA TCTGGAGAAG 
AGAACAGACA AGTCAGCTGT CTCAGGGGCT ATCCGACTTC AAATCAGTGT GGAGATCAAG 
GGGGAGGAGA AGGTAGCCCC ATACCATGTA CAATATACAT GTCTCCATGA GAACCTTTTC 
CATTACCTCA CAGACATTCA GGGCAGTGGA GGAGTCTGGA TCCCAGAGGC TCGGGGGGAC 
GATGCATGGA AGGTGTACTT TGATGAGACA GCCCAAGAAA TTGTGGATGA ATTCGCTATG 
CGCTATGGCA TCGAGTCCAT TTATCAGGCC ATGACGCACT TTGCATGTTT GTCATCCAAG 
TACATGTGTC CTGGTGTACC AGCCGTGATG AGCACCTTAC TGGCCAACAT CAATGCCTAT 
TATGCTCACA CAACTGCCTC CACTAACGTC TCTGCATCTG ATCGTTTTGC AGCCTCCAAC 
TTTGGTAAAG AAAGATTTGT AAAACTGCTG GACCAGCTAC ACAACTCACT GAGGATAGAC 
CTCTCTACAT ACAGGAATAA CTTTCCTGCT GGGAGCCCTG AGCGGCTTCA GGATTTAAAG 
TCTACAGTGG ATTTGCTGAC CAGCATTACT TTCTTCAGGA TGAAGGTGCA AGAACTTCAG 
AGCCCTCCAA GAGCCAGCCA GGTGGTAAAG GACTGTGTGA AAGCCTGCTT GAACTCTACA 
TATGAATACA TCTTCAACAA CTGCCATGAC CTCTACAGTC ACCAGTATCA GCTGCAGGAA 
CAACCTCTAG AGGAACCAGG GCCCAGCATT CGGAACTTGG ATTTCTGGCC CAAACTTATC 
ACACTCATTG TTTCGATCAT AGAGGAGGAT AAGAATTCCT ACACACCTGT TCTGAGCCAG 
TTTCCTCAGG AGTTGAACGT AGGAAAAGTC AGTGCAGAAG TGATGTGGCA TCTGTTTGCT 
CAAGACATGA AATATGCACT GGAGGAACAC GAGAAAGACC GGCTGTGTAA GAGTGCTGAC 
TACATGAACC TGCACTTCAA GGTGAAGTGG CTCCACAATG AATATGTGCG CGATCTGCCT 
GCCCTCCAGG GGCAAGTGCC TGAGTACCCA GCGTGGTTTG AGCAGTTTGT GCTACAGTGG 
CTAGATGAGA ATGAAGATGT GTCACTGGAA TTCCTTCGTG GGGCCTTGGA ACGAGATAAG 
AAGGATGGGT TCCAGCAGAC ATCAGAGCAT GCCTTGTTTT CCTGTTCTGT GGTGGATGTC 
TTTACACAAC TCAACCAGAG CTTTGAGATC ATCCGGAAGT TGGAATGTCC AGACCCCAAC 
ATCCTTGCCC ACTACATGAG AAGGTTTGCT AAGACCATTG GGAAAGTGCT GATGCAGTAT 
GCAGACATCT TATCAAAGAA CTTCCCAGCT TACTGCACAA AGGAGAGACT GCCCTGTATC 
CTGATGAACA ACATGCAGCA ACTGAGGGTC CAGCTGGAGA AAATGTTTGA GGCCATGGGA 
GGCAAAGAGC TGGACTCCGA AGCTGCAGAC AGTCTGAAGG AGCTGCAGGT GAAACTGAAT 
ACAGTTCTGG ATGAGCTTAG CATGGTGTTT GGAAACAGCT TCCAGGTGCG GATTGATGAA 
TGTGTTCGAC AAATGGCTGA CATCCTGGGC CAAGTTCGGG GCACAGGGAA TGCATCCCCT 
AACGCCAGGG CCTCGGTGGC TCAGGATGCA GATAGTGTGC TGCGACCTCT CATGGACTTC 
CTGGATGGCA ACCTCACGCT GTTTGCCACT GTGTGTGAGA AGACAGTTCT GAAGCGGGTC 
CTGAAGGAGC TCTGGCGGGT GGTGATGAAC ACCATGGAGC GGGTTATTGT CCTGCCCCCA 
CTCACTGACC AGACGGGCAC CCAGCTGATC TTAACTGCTG CCAAGGAGCT GAGCCAGCTT 
TCCAAACTCA AGGACCACAT GGTACGGGAG GAAACACGGA ATCTCACTCC AAAGCAGTGT 
GCTGTCCTCG ACTTGGCCTT GGACACCATC AAGCAATACT TCCATGCAGG AGGCAATGGT 
CTGAAGAAAA CCTTCCTGGA AAAGAGCCCA GACCTGCAGT CTCTGCGTTA TGCCCTCTCT 
CTGTATACAC AGACTACAGA CACTCTCATC AAGACATTTG TGCGCTCACA GACTGCCCAG 
GTACATGATG GGAAAGGTAT TAGGTTTACT GCTAACGAGG ACATTCGGCC AGAAAAGGGG 
GCTGGTGTGG ATGACCCCGT GGGAGAAGTC TCTATTCAGG TGGACTTATT TACACATCCT 
GGCACTGGGG AGCATAAGGT CACAGTGAAA GTGGTAGCAG CCAATGACCT CAAGTGGCAG 
ACAGCGGGTA TGTTCCGGCC TTTTGTGGAA GTGACCATGG TTGGCCCACA CCAAAGTGAT 
AAGAAGAGGA AGTTCACAAC CAAGTCTAAA AGTAACAACT GGACTCCCAA GTACAACGAG 
ACGTTTCACT TCCTCCTGGG AAATGAAGAG GGTCCAGAGG CCTATGAATT GCAGATCTGT 
GTGAAGGATT ACTGCTTTGC CCGGGAGGAT CGTGTGATAG GACTGGCAGT GATGCCCCTG 
AGGGATGTGG CAGCTAAGGG CAGCTGTGCC TGCTGGTGCC CACTGGGCCG GAAGATCCAT 
ATGGATGAGA CAGGCATGAC CATTCTCCGG ATTCTGTCTC AGAGGAGCAA TGACGAGGTA 
GCCCGAGAGT TCGTGAAGCT CAAATCAGAG TCTCGATCCA CAGAGGAAGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_030253406.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109266.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant X12, mRNA.

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CloneID	OMu80939
Clone ID Related Accession (Same CDS sequence)	XM_017320118.1 , XM_030253406.1 , XM_017320118.2
Accession Version	XM_030253406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAT CTGTGGAACT AAAAATCCAA CTCCTCATAA AATCTTGCTT GATACAAGAT 
TTGAGCTGCC TTTTGACATC CCAGAGGAGG AAGCCAGATA TTGGACCTAC AAATTGGAGC 
AAATCAATGC CTTGGCAGAT GATAATGAGT ATTCTAGTCA AGAAGAAAGC CAGAGGAAGC 
CATTACCCAC TGCTGCCGCC CAGTGTTGTC ACTGGACCTA TTTGGGCTGG GGAGAGCATC 
AGAGTACCCT TTGAAGACCC TGATAGTGCC GTTGATGACC GAGACAGTGA CTACCGCAGT 
GAGACCAGCA ATAGCGCCCC ACCTCCTTAC CACACGACTA CCCAGCCCAA CGCTTCTGTA 
CACCAGTTCC CTGTTCCTGT GCGGCTGCCA CAGCAGCTCT TTCTTCAAGG CAGTTCCCAT 
GACTCTTGCA ATGACTCTAT GCAGAGTTAC GACCTTGATT ATCCAGAGCG TCGGGCCCTC 
AGCCCTACCA GCAGTAGTAG GTATGGCTCC TCCTGTAATG TGAGTCAAGG AAGCTCTCTG 
CTGAGTGAGC TGGACCAGTA TCATGAGCAA GATGATGATG GTCGGGAGAG GGACTCCATT 
CATTCTAGTC ATAGCTATGG CAGCCTCTCC AAAGATGGCC AGGCTGGTTT GGGAGAACAA 
GAGAAAGCCT TGGAAGTGAC ATGTGAATCA GAAAAAGAGA AAACAGGTGA ATCCAAGGAG 
ATGAGAGATG ATGCTACAAT CCACCCTCCT TCAGATCTGG TGCTGCATAA GGACCATGTC 
CTAGGACCCC AGGAGAGTCT TCCTGAGGAG ACCGCATCAT CGCCATTTAC CCAGGCCAGA 
GCACACTGGT TCCGAGCGGT TACCAAGGTC CGACTCCAAC TGCAGGAGAT TTCAGATGAT 
GGTGACCCCT CCTTGCCTCA GTGGCTCCCA GAAGGGCCAG CTGGAGGGCT CTATGGCATT 
GACAGCATGC CAGATCTACG AAGGAAGAAG CCACTGCCAC TTGTCAGTGA TCTGGCTATG 
TCACTGGTCC AGTCACGGAA AGCTGGGATT ACATCTGCTA TGGCTACACG TACCTCTCTC 
AAGGATGAAG AACTGAAATC TCATGTGTAT AAGAAAACTC TGCAGGCCTT AATCTACCCC 
ATCTCGTGTA CCACGCCCCA CAACTTTGAG GTCTGGTCTG CCACTACACC TACCTATTGC 
TATGAGTGTG AAGGCTTGCT CTGGGGCCTT GCCCGGCAAG GCATGCGCTG CAGCGAGTGT 
GGAGTCAAGT GCCATGAGAA GTGCCAGGAC CTGCTCAATG CTGACTGCCT ACAGCGGGCT 
GCTGAAAAGA GCTCTAAGCA TGGAGCTGAG GACCGAACTC AGAACATTAT CATGGCCATG 
AAGGACCGTA TGAAGATCCG AGAACGGAAT AAACCAGAGA TCTTTGAGGT TATCCGGGAT 
GTGTTCACAG TGAGCAAAGT TGCGCATGTG CAGCAGATGA AAACAGTAAA GCAGAGTGTC 
CTGGATGGCA CCTCCAAGTG GTCAGCCAAA ATTACCATCA CGGTGGTTTG TGCCCAGGGC 
CTACAAGCCA AAGACAAGAC AGGATCCAGT GACCCTTATG TGACTGTGCA AGTTGGGAAA 
ACCAAGAAGC GCACCAAGAC CATTTTTGGA AATTTGAATC CTGTTTGGGA AGAGAAGTTC 
CATTTTGAGT GCCACAACTC TTCTGATCGA ATTAAGGTAC GCGTGTGGGA TGAGGATGAT 
GACATCAAGT CCAGAGTAAA GCAGCGGCTA AAGCGAGAGT CAGATGATTT CCTTGGCCAA 
ACTATCATTG AAGTTCGAAC GCTGAGTGGA GAGATGGATG TCTGGTACAA TCTGGAGAAG 
AGAACAGACA AGTCAGCTGT CTCAGGGGCT ATCCGACTTC AAATCAGTGT GGAGATCAAG 
GGGGAGGAGA AGGTAGCCCC ATACCATGTA CAATATACAT GTCTCCATGA GAACCTTTTC 
CATTACCTCA CAGACATTCA GGGCAGTGGA GGAGTCTGGA TCCCAGAGGC TCGGGGGGAC 
GATGCATGGA AGGTGTACTT TGATGAGACA GCCCAAGAAA TTGTGGATGA ATTCGCTATG 
CGCTATGGCA TCGAGTCCAT TTATCAGGCC ATGACGCACT TTGCATGTTT GTCATCCAAG 
TACATGTGTC CTGGTGTACC AGCCGTGATG AGCACCTTAC TGGCCAACAT CAATGCCTAT 
TATGCTCACA CAACTGCCTC CACTAACGTC TCTGCATCTG ATCGTTTTGC AGCCTCCAAC 
TTTGGTAAAG AAAGATTTGT AAAACTGCTG GACCAGCTAC ACAACTCACT GAGGATAGAC 
CTCTCTACAT ACAGGAATAA CTTTCCTGCT GGGAGCCCTG AGCGGCTTCA GGATTTAAAG 
TCTACAGTGG ATTTGCTGAC CAGCATTACT TTCTTCAGGA TGAAGGTGCA AGAACTTCAG 
AGCCCTCCAA GAGCCAGCCA GGTGGTAAAG GACTGTGTGA AAGCCTGCTT GAACTCTACA 
TATGAATACA TCTTCAACAA CTGCCATGAC CTCTACAGTC ACCAGTATCA GCTGCAGGAA 
CAACCTCTAG AGGAACCAGG GCCCAGCATT CGGAACTTGG ATTTCTGGCC CAAACTTATC 
ACACTCATTG TTTCGATCAT AGAGGAGGAT AAGAATTCCT ACACACCTGT TCTGAGCCAG 
TTTCCTCAGG AGTTGAACGT AGGAAAAGTC AGTGCAGAAG TGATGTGGCA TCTGTTTGCT 
CAAGACATGA AATATGCACT GGAGGAACAC GAGAAAGACC GGCTGTGTAA GAGTGCTGAC 
TACATGAACC TGCACTTCAA GGTGAAGTGG CTCCACAATG AATATGTGCG CGATCTGCCT 
GCCCTCCAGG GGCAAGTGCC TGAGTACCCA GCGTGGTTTG AGCAGTTTGT GCTACAGTGG 
CTAGATGAGA ATGAAGATGT GTCACTGGAA TTCCTTCGTG GGGCCTTGGA ACGAGATAAG 
AAGGATGGGT TCCAGCAGAC ATCAGAGCAT GCCTTGTTTT CCTGTTCTGT GGTGGATGTC 
TTTACACAAC TCAACCAGAG CTTTGAGATC ATCCGGAAGT TGGAATGTCC AGACCCCAAC 
ATCCTTGCCC ACTACATGAG AAGGTTTGCT AAGACCATTG GGAAAGTGCT GATGCAGTAT 
GCAGACATCT TATCAAAGAA CTTCCCAGCT TACTGCACAA AGGAGAGACT GCCCTGTATC 
CTGATGAACA ACATGCAGCA ACTGAGGGTC CAGCTGGAGA AAATGTTTGA GGCCATGGGA 
GGCAAAGAGC TGGACTCCGA AGCTGCAGAC AGTCTGAAGG AGCTGCAGGT GAAACTGAAT 
ACAGTTCTGG ATGAGCTTAG CATGGTGTTT GGAAACAGCT TCCAGGTGCG GATTGATGAA 
TGTGTTCGAC AAATGGCTGA CATCCTGGGC CAAGTTCGGG GCACAGGGAA TGCATCCCCT 
AACGCCAGGG CCTCGGTGGC TCAGGATGCA GATAGTGTGC TGCGACCTCT CATGGACTTC 
CTGGATGGCA ACCTCACGCT GTTTGCCACT GTGTGTGAGA AGACAGTTCT GAAGCGGGTC 
CTGAAGGAGC TCTGGCGGGT GGTGATGAAC ACCATGGAGC GGGTTATTGT CCTGCCCCCA 
CTCACTGACC AGACGGGCAC CCAGCTGATC TTAACTGCTG CCAAGGAGCT GAGCCAGCTT 
TCCAAACTCA AGGACCACAT GGTACGGGAG GAAACACGGA ATCTCACTCC AAAGCAGTGT 
GCTGTCCTCG ACTTGGCCTT GGACACCATC AAGCAATACT TCCATGCAGG AGGCAATGGT 
CTGAAGAAAA CCTTCCTGGA AAAGAGCCCA GACCTGCAGT CTCTGCGTTA TGCCCTCTCT 
CTGTATACAC AGACTACAGA CACTCTCATC AAGACATTTG TGCGCTCACA GACTGCCCAG 
GTACATGATG GGAAAGGTAT TAGGTTTACT GCTAACGAGG ACATTCGGCC AGAAAAGGGG 
GCTGGTGTGG ATGACCCCGT GGGAGAAGTC TCTATTCAGG TGGACTTATT TACACATCCT 
GGCACTGGGG AGCATAAGGT CACAGTGAAA GTGGTAGCAG CCAATGACCT CAAGTGGCAG 
ACAGCGGGTA TGTTCCGGCC TTTTGTGGAA GTGACCATGG TTGGCCCACA CCAAAGTGAT 
AAGAAGAGGA AGTTCACAAC CAAGTCTAAA AGTAACAACT GGACTCCCAA GTACAACGAG 
ACGTTTCACT TCCTCCTGGG AAATGAAGAG GGTCCAGAGG CCTATGAATT GCAGATCTGT 
GTGAAGGATT ACTGCTTTGC CCGGGAGGAT CGTGTGATAG GACTGGCAGT GATGCCCCTG 
AGGGATGTGG CAGCTAAGGG CAGCTGTGCC TGCTGGTGCC CACTGGGCCG GAAGATCCAT 
ATGGATGAGA CAGGCATGAC CATTCTCCGG ATTCTGTCTC AGAGGAGCAA TGACGAGGTA 
GCCCGAGAGT TCGTGAAGCT CAAATCAGAG TCTCGATCCA CAGAGGAAGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109266.1
CDS	383..4939
Translation	MMRSVELKIQLLIKSCLIQDLSCLLTSQRRKPDIGPTNWSKSMPWQMIMSILVKKKARGSHYPLLPPSVVTGPIWAGESIRVPFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_030253406.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253406.1

ORF Insert Sequence:

ATGATGAGAT CTGTGGAACT AAAAATCCAA CTCCTCATAA AATCTTGCTT GATACAAGAT 
TTGAGCTGCC TTTTGACATC CCAGAGGAGG AAGCCAGATA TTGGACCTAC AAATTGGAGC 
AAATCAATGC CTTGGCAGAT GATAATGAGT ATTCTAGTCA AGAAGAAAGC CAGAGGAAGC 
CATTACCCAC TGCTGCCGCC CAGTGTTGTC ACTGGACCTA TTTGGGCTGG GGAGAGCATC 
AGAGTACCCT TTGAAGACCC TGATAGTGCC GTTGATGACC GAGACAGTGA CTACCGCAGT 
GAGACCAGCA ATAGCGCCCC ACCTCCTTAC CACACGACTA CCCAGCCCAA CGCTTCTGTA 
CACCAGTTCC CTGTTCCTGT GCGGCTGCCA CAGCAGCTCT TTCTTCAAGG CAGTTCCCAT 
GACTCTTGCA ATGACTCTAT GCAGAGTTAC GACCTTGATT ATCCAGAGCG TCGGGCCCTC 
AGCCCTACCA GCAGTAGTAG GTATGGCTCC TCCTGTAATG TGAGTCAAGG AAGCTCTCTG 
CTGAGTGAGC TGGACCAGTA TCATGAGCAA GATGATGATG GTCGGGAGAG GGACTCCATT 
CATTCTAGTC ATAGCTATGG CAGCCTCTCC AAAGATGGCC AGGCTGGTTT GGGAGAACAA 
GAGAAAGCCT TGGAAGTGAC ATGTGAATCA GAAAAAGAGA AAACAGGTGA ATCCAAGGAG 
ATGAGAGATG ATGCTACAAT CCACCCTCCT TCAGATCTGG TGCTGCATAA GGACCATGTC 
CTAGGACCCC AGGAGAGTCT TCCTGAGGAG ACCGCATCAT CGCCATTTAC CCAGGCCAGA 
GCACACTGGT TCCGAGCGGT TACCAAGGTC CGACTCCAAC TGCAGGAGAT TTCAGATGAT 
GGTGACCCCT CCTTGCCTCA GTGGCTCCCA GAAGGGCCAG CTGGAGGGCT CTATGGCATT 
GACAGCATGC CAGATCTACG AAGGAAGAAG CCACTGCCAC TTGTCAGTGA TCTGGCTATG 
TCACTGGTCC AGTCACGGAA AGCTGGGATT ACATCTGCTA TGGCTACACG TACCTCTCTC 
AAGGATGAAG AACTGAAATC TCATGTGTAT AAGAAAACTC TGCAGGCCTT AATCTACCCC 
ATCTCGTGTA CCACGCCCCA CAACTTTGAG GTCTGGTCTG CCACTACACC TACCTATTGC 
TATGAGTGTG AAGGCTTGCT CTGGGGCCTT GCCCGGCAAG GCATGCGCTG CAGCGAGTGT 
GGAGTCAAGT GCCATGAGAA GTGCCAGGAC CTGCTCAATG CTGACTGCCT ACAGCGGGCT 
GCTGAAAAGA GCTCTAAGCA TGGAGCTGAG GACCGAACTC AGAACATTAT CATGGCCATG 
AAGGACCGTA TGAAGATCCG AGAACGGAAT AAACCAGAGA TCTTTGAGGT TATCCGGGAT 
GTGTTCACAG TGAGCAAAGT TGCGCATGTG CAGCAGATGA AAACAGTAAA GCAGAGTGTC 
CTGGATGGCA CCTCCAAGTG GTCAGCCAAA ATTACCATCA CGGTGGTTTG TGCCCAGGGC 
CTACAAGCCA AAGACAAGAC AGGATCCAGT GACCCTTATG TGACTGTGCA AGTTGGGAAA 
ACCAAGAAGC GCACCAAGAC CATTTTTGGA AATTTGAATC CTGTTTGGGA AGAGAAGTTC 
CATTTTGAGT GCCACAACTC TTCTGATCGA ATTAAGGTAC GCGTGTGGGA TGAGGATGAT 
GACATCAAGT CCAGAGTAAA GCAGCGGCTA AAGCGAGAGT CAGATGATTT CCTTGGCCAA 
ACTATCATTG AAGTTCGAAC GCTGAGTGGA GAGATGGATG TCTGGTACAA TCTGGAGAAG 
AGAACAGACA AGTCAGCTGT CTCAGGGGCT ATCCGACTTC AAATCAGTGT GGAGATCAAG 
GGGGAGGAGA AGGTAGCCCC ATACCATGTA CAATATACAT GTCTCCATGA GAACCTTTTC 
CATTACCTCA CAGACATTCA GGGCAGTGGA GGAGTCTGGA TCCCAGAGGC TCGGGGGGAC 
GATGCATGGA AGGTGTACTT TGATGAGACA GCCCAAGAAA TTGTGGATGA ATTCGCTATG 
CGCTATGGCA TCGAGTCCAT TTATCAGGCC ATGACGCACT TTGCATGTTT GTCATCCAAG 
TACATGTGTC CTGGTGTACC AGCCGTGATG AGCACCTTAC TGGCCAACAT CAATGCCTAT 
TATGCTCACA CAACTGCCTC CACTAACGTC TCTGCATCTG ATCGTTTTGC AGCCTCCAAC 
TTTGGTAAAG AAAGATTTGT AAAACTGCTG GACCAGCTAC ACAACTCACT GAGGATAGAC 
CTCTCTACAT ACAGGAATAA CTTTCCTGCT GGGAGCCCTG AGCGGCTTCA GGATTTAAAG 
TCTACAGTGG ATTTGCTGAC CAGCATTACT TTCTTCAGGA TGAAGGTGCA AGAACTTCAG 
AGCCCTCCAA GAGCCAGCCA GGTGGTAAAG GACTGTGTGA AAGCCTGCTT GAACTCTACA 
TATGAATACA TCTTCAACAA CTGCCATGAC CTCTACAGTC ACCAGTATCA GCTGCAGGAA 
CAACCTCTAG AGGAACCAGG GCCCAGCATT CGGAACTTGG ATTTCTGGCC CAAACTTATC 
ACACTCATTG TTTCGATCAT AGAGGAGGAT AAGAATTCCT ACACACCTGT TCTGAGCCAG 
TTTCCTCAGG AGTTGAACGT AGGAAAAGTC AGTGCAGAAG TGATGTGGCA TCTGTTTGCT 
CAAGACATGA AATATGCACT GGAGGAACAC GAGAAAGACC GGCTGTGTAA GAGTGCTGAC 
TACATGAACC TGCACTTCAA GGTGAAGTGG CTCCACAATG AATATGTGCG CGATCTGCCT 
GCCCTCCAGG GGCAAGTGCC TGAGTACCCA GCGTGGTTTG AGCAGTTTGT GCTACAGTGG 
CTAGATGAGA ATGAAGATGT GTCACTGGAA TTCCTTCGTG GGGCCTTGGA ACGAGATAAG 
AAGGATGGGT TCCAGCAGAC ATCAGAGCAT GCCTTGTTTT CCTGTTCTGT GGTGGATGTC 
TTTACACAAC TCAACCAGAG CTTTGAGATC ATCCGGAAGT TGGAATGTCC AGACCCCAAC 
ATCCTTGCCC ACTACATGAG AAGGTTTGCT AAGACCATTG GGAAAGTGCT GATGCAGTAT 
GCAGACATCT TATCAAAGAA CTTCCCAGCT TACTGCACAA AGGAGAGACT GCCCTGTATC 
CTGATGAACA ACATGCAGCA ACTGAGGGTC CAGCTGGAGA AAATGTTTGA GGCCATGGGA 
GGCAAAGAGC TGGACTCCGA AGCTGCAGAC AGTCTGAAGG AGCTGCAGGT GAAACTGAAT 
ACAGTTCTGG ATGAGCTTAG CATGGTGTTT GGAAACAGCT TCCAGGTGCG GATTGATGAA 
TGTGTTCGAC AAATGGCTGA CATCCTGGGC CAAGTTCGGG GCACAGGGAA TGCATCCCCT 
AACGCCAGGG CCTCGGTGGC TCAGGATGCA GATAGTGTGC TGCGACCTCT CATGGACTTC 
CTGGATGGCA ACCTCACGCT GTTTGCCACT GTGTGTGAGA AGACAGTTCT GAAGCGGGTC 
CTGAAGGAGC TCTGGCGGGT GGTGATGAAC ACCATGGAGC GGGTTATTGT CCTGCCCCCA 
CTCACTGACC AGACGGGCAC CCAGCTGATC TTAACTGCTG CCAAGGAGCT GAGCCAGCTT 
TCCAAACTCA AGGACCACAT GGTACGGGAG GAAACACGGA ATCTCACTCC AAAGCAGTGT 
GCTGTCCTCG ACTTGGCCTT GGACACCATC AAGCAATACT TCCATGCAGG AGGCAATGGT 
CTGAAGAAAA CCTTCCTGGA AAAGAGCCCA GACCTGCAGT CTCTGCGTTA TGCCCTCTCT 
CTGTATACAC AGACTACAGA CACTCTCATC AAGACATTTG TGCGCTCACA GACTGCCCAG 
GTACATGATG GGAAAGGTAT TAGGTTTACT GCTAACGAGG ACATTCGGCC AGAAAAGGGG 
GCTGGTGTGG ATGACCCCGT GGGAGAAGTC TCTATTCAGG TGGACTTATT TACACATCCT 
GGCACTGGGG AGCATAAGGT CACAGTGAAA GTGGTAGCAG CCAATGACCT CAAGTGGCAG 
ACAGCGGGTA TGTTCCGGCC TTTTGTGGAA GTGACCATGG TTGGCCCACA CCAAAGTGAT 
AAGAAGAGGA AGTTCACAAC CAAGTCTAAA AGTAACAACT GGACTCCCAA GTACAACGAG 
ACGTTTCACT TCCTCCTGGG AAATGAAGAG GGTCCAGAGG CCTATGAATT GCAGATCTGT 
GTGAAGGATT ACTGCTTTGC CCGGGAGGAT CGTGTGATAG GACTGGCAGT GATGCCCCTG 
AGGGATGTGG CAGCTAAGGG CAGCTGTGCC TGCTGGTGCC CACTGGGCCG GAAGATCCAT 
ATGGATGAGA CAGGCATGAC CATTCTCCGG ATTCTGTCTC AGAGGAGCAA TGACGAGGTA 
GCCCGAGAGT TCGTGAAGCT CAAATCAGAG TCTCGATCCA CAGAGGAAGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_017320118.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175607.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant X11, mRNA.

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CloneID	OMu80939
Clone ID Related Accession (Same CDS sequence)	XM_017320118.1 , XM_030253406.1 , XM_017320118.2
Accession Version	XM_017320118.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAT CTGTGGAACT AAAAATCCAA CTCCTCATAA AATCTTGCTT GATACAAGAT 
TTGAGCTGCC TTTTGACATC CCAGAGGAGG AAGCCAGATA TTGGACCTAC AAATTGGAGC 
AAATCAATGC CTTGGCAGAT GATAATGAGT ATTCTAGTCA AGAAGAAAGC CAGAGGAAGC 
CATTACCCAC TGCTGCCGCC CAGTGTTGTC ACTGGACCTA TTTGGGCTGG GGAGAGCATC 
AGAGTACCCT TTGAAGACCC TGATAGTGCC GTTGATGACC GAGACAGTGA CTACCGCAGT 
GAGACCAGCA ATAGCGCCCC ACCTCCTTAC CACACGACTA CCCAGCCCAA CGCTTCTGTA 
CACCAGTTCC CTGTTCCTGT GCGGCTGCCA CAGCAGCTCT TTCTTCAAGG CAGTTCCCAT 
GACTCTTGCA ATGACTCTAT GCAGAGTTAC GACCTTGATT ATCCAGAGCG TCGGGCCCTC 
AGCCCTACCA GCAGTAGTAG GTATGGCTCC TCCTGTAATG TGAGTCAAGG AAGCTCTCTG 
CTGAGTGAGC TGGACCAGTA TCATGAGCAA GATGATGATG GTCGGGAGAG GGACTCCATT 
CATTCTAGTC ATAGCTATGG CAGCCTCTCC AAAGATGGCC AGGCTGGTTT GGGAGAACAA 
GAGAAAGCCT TGGAAGTGAC ATGTGAATCA GAAAAAGAGA AAACAGGTGA ATCCAAGGAG 
ATGAGAGATG ATGCTACAAT CCACCCTCCT TCAGATCTGG TGCTGCATAA GGACCATGTC 
CTAGGACCCC AGGAGAGTCT TCCTGAGGAG ACCGCATCAT CGCCATTTAC CCAGGCCAGA 
GCACACTGGT TCCGAGCGGT TACCAAGGTC CGACTCCAAC TGCAGGAGAT TTCAGATGAT 
GGTGACCCCT CCTTGCCTCA GTGGCTCCCA GAAGGGCCAG CTGGAGGGCT CTATGGCATT 
GACAGCATGC CAGATCTACG AAGGAAGAAG CCACTGCCAC TTGTCAGTGA TCTGGCTATG 
TCACTGGTCC AGTCACGGAA AGCTGGGATT ACATCTGCTA TGGCTACACG TACCTCTCTC 
AAGGATGAAG AACTGAAATC TCATGTGTAT AAGAAAACTC TGCAGGCCTT AATCTACCCC 
ATCTCGTGTA CCACGCCCCA CAACTTTGAG GTCTGGTCTG CCACTACACC TACCTATTGC 
TATGAGTGTG AAGGCTTGCT CTGGGGCCTT GCCCGGCAAG GCATGCGCTG CAGCGAGTGT 
GGAGTCAAGT GCCATGAGAA GTGCCAGGAC CTGCTCAATG CTGACTGCCT ACAGCGGGCT 
GCTGAAAAGA GCTCTAAGCA TGGAGCTGAG GACCGAACTC AGAACATTAT CATGGCCATG 
AAGGACCGTA TGAAGATCCG AGAACGGAAT AAACCAGAGA TCTTTGAGGT TATCCGGGAT 
GTGTTCACAG TGAGCAAAGT TGCGCATGTG CAGCAGATGA AAACAGTAAA GCAGAGTGTC 
CTGGATGGCA CCTCCAAGTG GTCAGCCAAA ATTACCATCA CGGTGGTTTG TGCCCAGGGC 
CTACAAGCCA AAGACAAGAC AGGATCCAGT GACCCTTATG TGACTGTGCA AGTTGGGAAA 
ACCAAGAAGC GCACCAAGAC CATTTTTGGA AATTTGAATC CTGTTTGGGA AGAGAAGTTC 
CATTTTGAGT GCCACAACTC TTCTGATCGA ATTAAGGTAC GCGTGTGGGA TGAGGATGAT 
GACATCAAGT CCAGAGTAAA GCAGCGGCTA AAGCGAGAGT CAGATGATTT CCTTGGCCAA 
ACTATCATTG AAGTTCGAAC GCTGAGTGGA GAGATGGATG TCTGGTACAA TCTGGAGAAG 
AGAACAGACA AGTCAGCTGT CTCAGGGGCT ATCCGACTTC AAATCAGTGT GGAGATCAAG 
GGGGAGGAGA AGGTAGCCCC ATACCATGTA CAATATACAT GTCTCCATGA GAACCTTTTC 
CATTACCTCA CAGACATTCA GGGCAGTGGA GGAGTCTGGA TCCCAGAGGC TCGGGGGGAC 
GATGCATGGA AGGTGTACTT TGATGAGACA GCCCAAGAAA TTGTGGATGA ATTCGCTATG 
CGCTATGGCA TCGAGTCCAT TTATCAGGCC ATGACGCACT TTGCATGTTT GTCATCCAAG 
TACATGTGTC CTGGTGTACC AGCCGTGATG AGCACCTTAC TGGCCAACAT CAATGCCTAT 
TATGCTCACA CAACTGCCTC CACTAACGTC TCTGCATCTG ATCGTTTTGC AGCCTCCAAC 
TTTGGTAAAG AAAGATTTGT AAAACTGCTG GACCAGCTAC ACAACTCACT GAGGATAGAC 
CTCTCTACAT ACAGGAATAA CTTTCCTGCT GGGAGCCCTG AGCGGCTTCA GGATTTAAAG 
TCTACAGTGG ATTTGCTGAC CAGCATTACT TTCTTCAGGA TGAAGGTGCA AGAACTTCAG 
AGCCCTCCAA GAGCCAGCCA GGTGGTAAAG GACTGTGTGA AAGCCTGCTT GAACTCTACA 
TATGAATACA TCTTCAACAA CTGCCATGAC CTCTACAGTC ACCAGTATCA GCTGCAGGAA 
CAACCTCTAG AGGAACCAGG GCCCAGCATT CGGAACTTGG ATTTCTGGCC CAAACTTATC 
ACACTCATTG TTTCGATCAT AGAGGAGGAT AAGAATTCCT ACACACCTGT TCTGAGCCAG 
TTTCCTCAGG AGTTGAACGT AGGAAAAGTC AGTGCAGAAG TGATGTGGCA TCTGTTTGCT 
CAAGACATGA AATATGCACT GGAGGAACAC GAGAAAGACC GGCTGTGTAA GAGTGCTGAC 
TACATGAACC TGCACTTCAA GGTGAAGTGG CTCCACAATG AATATGTGCG CGATCTGCCT 
GCCCTCCAGG GGCAAGTGCC TGAGTACCCA GCGTGGTTTG AGCAGTTTGT GCTACAGTGG 
CTAGATGAGA ATGAAGATGT GTCACTGGAA TTCCTTCGTG GGGCCTTGGA ACGAGATAAG 
AAGGATGGGT TCCAGCAGAC ATCAGAGCAT GCCTTGTTTT CCTGTTCTGT GGTGGATGTC 
TTTACACAAC TCAACCAGAG CTTTGAGATC ATCCGGAAGT TGGAATGTCC AGACCCCAAC 
ATCCTTGCCC ACTACATGAG AAGGTTTGCT AAGACCATTG GGAAAGTGCT GATGCAGTAT 
GCAGACATCT TATCAAAGAA CTTCCCAGCT TACTGCACAA AGGAGAGACT GCCCTGTATC 
CTGATGAACA ACATGCAGCA ACTGAGGGTC CAGCTGGAGA AAATGTTTGA GGCCATGGGA 
GGCAAAGAGC TGGACTCCGA AGCTGCAGAC AGTCTGAAGG AGCTGCAGGT GAAACTGAAT 
ACAGTTCTGG ATGAGCTTAG CATGGTGTTT GGAAACAGCT TCCAGGTGCG GATTGATGAA 
TGTGTTCGAC AAATGGCTGA CATCCTGGGC CAAGTTCGGG GCACAGGGAA TGCATCCCCT 
AACGCCAGGG CCTCGGTGGC TCAGGATGCA GATAGTGTGC TGCGACCTCT CATGGACTTC 
CTGGATGGCA ACCTCACGCT GTTTGCCACT GTGTGTGAGA AGACAGTTCT GAAGCGGGTC 
CTGAAGGAGC TCTGGCGGGT GGTGATGAAC ACCATGGAGC GGGTTATTGT CCTGCCCCCA 
CTCACTGACC AGACGGGCAC CCAGCTGATC TTAACTGCTG CCAAGGAGCT GAGCCAGCTT 
TCCAAACTCA AGGACCACAT GGTACGGGAG GAAACACGGA ATCTCACTCC AAAGCAGTGT 
GCTGTCCTCG ACTTGGCCTT GGACACCATC AAGCAATACT TCCATGCAGG AGGCAATGGT 
CTGAAGAAAA CCTTCCTGGA AAAGAGCCCA GACCTGCAGT CTCTGCGTTA TGCCCTCTCT 
CTGTATACAC AGACTACAGA CACTCTCATC AAGACATTTG TGCGCTCACA GACTGCCCAG 
GTACATGATG GGAAAGGTAT TAGGTTTACT GCTAACGAGG ACATTCGGCC AGAAAAGGGG 
GCTGGTGTGG ATGACCCCGT GGGAGAAGTC TCTATTCAGG TGGACTTATT TACACATCCT 
GGCACTGGGG AGCATAAGGT CACAGTGAAA GTGGTAGCAG CCAATGACCT CAAGTGGCAG 
ACAGCGGGTA TGTTCCGGCC TTTTGTGGAA GTGACCATGG TTGGCCCACA CCAAAGTGAT 
AAGAAGAGGA AGTTCACAAC CAAGTCTAAA AGTAACAACT GGACTCCCAA GTACAACGAG 
ACGTTTCACT TCCTCCTGGG AAATGAAGAG GGTCCAGAGG CCTATGAATT GCAGATCTGT 
GTGAAGGATT ACTGCTTTGC CCGGGAGGAT CGTGTGATAG GACTGGCAGT GATGCCCCTG 
AGGGATGTGG CAGCTAAGGG CAGCTGTGCC TGCTGGTGCC CACTGGGCCG GAAGATCCAT 
ATGGATGAGA CAGGCATGAC CATTCTCCGG ATTCTGTCTC AGAGGAGCAA TGACGAGGTA 
GCCCGAGAGT TCGTGAAGCT CAAATCAGAG TCTCGATCCA CAGAGGAAGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175607.1
CDS	654..5210
Translation	MMRSVELKIQLLIKSCLIQDLSCLLTSQRRKPDIGPTNWSKSMPWQMIMSILVKKKARGSHYPLLPPSVVTGPIWAGESIRVPFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_017320118.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320118.2

ORF Insert Sequence:

ATGATGAGAT CTGTGGAACT AAAAATCCAA CTCCTCATAA AATCTTGCTT GATACAAGAT 
TTGAGCTGCC TTTTGACATC CCAGAGGAGG AAGCCAGATA TTGGACCTAC AAATTGGAGC 
AAATCAATGC CTTGGCAGAT GATAATGAGT ATTCTAGTCA AGAAGAAAGC CAGAGGAAGC 
CATTACCCAC TGCTGCCGCC CAGTGTTGTC ACTGGACCTA TTTGGGCTGG GGAGAGCATC 
AGAGTACCCT TTGAAGACCC TGATAGTGCC GTTGATGACC GAGACAGTGA CTACCGCAGT 
GAGACCAGCA ATAGCGCCCC ACCTCCTTAC CACACGACTA CCCAGCCCAA CGCTTCTGTA 
CACCAGTTCC CTGTTCCTGT GCGGCTGCCA CAGCAGCTCT TTCTTCAAGG CAGTTCCCAT 
GACTCTTGCA ATGACTCTAT GCAGAGTTAC GACCTTGATT ATCCAGAGCG TCGGGCCCTC 
AGCCCTACCA GCAGTAGTAG GTATGGCTCC TCCTGTAATG TGAGTCAAGG AAGCTCTCTG 
CTGAGTGAGC TGGACCAGTA TCATGAGCAA GATGATGATG GTCGGGAGAG GGACTCCATT 
CATTCTAGTC ATAGCTATGG CAGCCTCTCC AAAGATGGCC AGGCTGGTTT GGGAGAACAA 
GAGAAAGCCT TGGAAGTGAC ATGTGAATCA GAAAAAGAGA AAACAGGTGA ATCCAAGGAG 
ATGAGAGATG ATGCTACAAT CCACCCTCCT TCAGATCTGG TGCTGCATAA GGACCATGTC 
CTAGGACCCC AGGAGAGTCT TCCTGAGGAG ACCGCATCAT CGCCATTTAC CCAGGCCAGA 
GCACACTGGT TCCGAGCGGT TACCAAGGTC CGACTCCAAC TGCAGGAGAT TTCAGATGAT 
GGTGACCCCT CCTTGCCTCA GTGGCTCCCA GAAGGGCCAG CTGGAGGGCT CTATGGCATT 
GACAGCATGC CAGATCTACG AAGGAAGAAG CCACTGCCAC TTGTCAGTGA TCTGGCTATG 
TCACTGGTCC AGTCACGGAA AGCTGGGATT ACATCTGCTA TGGCTACACG TACCTCTCTC 
AAGGATGAAG AACTGAAATC TCATGTGTAT AAGAAAACTC TGCAGGCCTT AATCTACCCC 
ATCTCGTGTA CCACGCCCCA CAACTTTGAG GTCTGGTCTG CCACTACACC TACCTATTGC 
TATGAGTGTG AAGGCTTGCT CTGGGGCCTT GCCCGGCAAG GCATGCGCTG CAGCGAGTGT 
GGAGTCAAGT GCCATGAGAA GTGCCAGGAC CTGCTCAATG CTGACTGCCT ACAGCGGGCT 
GCTGAAAAGA GCTCTAAGCA TGGAGCTGAG GACCGAACTC AGAACATTAT CATGGCCATG 
AAGGACCGTA TGAAGATCCG AGAACGGAAT AAACCAGAGA TCTTTGAGGT TATCCGGGAT 
GTGTTCACAG TGAGCAAAGT TGCGCATGTG CAGCAGATGA AAACAGTAAA GCAGAGTGTC 
CTGGATGGCA CCTCCAAGTG GTCAGCCAAA ATTACCATCA CGGTGGTTTG TGCCCAGGGC 
CTACAAGCCA AAGACAAGAC AGGATCCAGT GACCCTTATG TGACTGTGCA AGTTGGGAAA 
ACCAAGAAGC GCACCAAGAC CATTTTTGGA AATTTGAATC CTGTTTGGGA AGAGAAGTTC 
CATTTTGAGT GCCACAACTC TTCTGATCGA ATTAAGGTAC GCGTGTGGGA TGAGGATGAT 
GACATCAAGT CCAGAGTAAA GCAGCGGCTA AAGCGAGAGT CAGATGATTT CCTTGGCCAA 
ACTATCATTG AAGTTCGAAC GCTGAGTGGA GAGATGGATG TCTGGTACAA TCTGGAGAAG 
AGAACAGACA AGTCAGCTGT CTCAGGGGCT ATCCGACTTC AAATCAGTGT GGAGATCAAG 
GGGGAGGAGA AGGTAGCCCC ATACCATGTA CAATATACAT GTCTCCATGA GAACCTTTTC 
CATTACCTCA CAGACATTCA GGGCAGTGGA GGAGTCTGGA TCCCAGAGGC TCGGGGGGAC 
GATGCATGGA AGGTGTACTT TGATGAGACA GCCCAAGAAA TTGTGGATGA ATTCGCTATG 
CGCTATGGCA TCGAGTCCAT TTATCAGGCC ATGACGCACT TTGCATGTTT GTCATCCAAG 
TACATGTGTC CTGGTGTACC AGCCGTGATG AGCACCTTAC TGGCCAACAT CAATGCCTAT 
TATGCTCACA CAACTGCCTC CACTAACGTC TCTGCATCTG ATCGTTTTGC AGCCTCCAAC 
TTTGGTAAAG AAAGATTTGT AAAACTGCTG GACCAGCTAC ACAACTCACT GAGGATAGAC 
CTCTCTACAT ACAGGAATAA CTTTCCTGCT GGGAGCCCTG AGCGGCTTCA GGATTTAAAG 
TCTACAGTGG ATTTGCTGAC CAGCATTACT TTCTTCAGGA TGAAGGTGCA AGAACTTCAG 
AGCCCTCCAA GAGCCAGCCA GGTGGTAAAG GACTGTGTGA AAGCCTGCTT GAACTCTACA 
TATGAATACA TCTTCAACAA CTGCCATGAC CTCTACAGTC ACCAGTATCA GCTGCAGGAA 
CAACCTCTAG AGGAACCAGG GCCCAGCATT CGGAACTTGG ATTTCTGGCC CAAACTTATC 
ACACTCATTG TTTCGATCAT AGAGGAGGAT AAGAATTCCT ACACACCTGT TCTGAGCCAG 
TTTCCTCAGG AGTTGAACGT AGGAAAAGTC AGTGCAGAAG TGATGTGGCA TCTGTTTGCT 
CAAGACATGA AATATGCACT GGAGGAACAC GAGAAAGACC GGCTGTGTAA GAGTGCTGAC 
TACATGAACC TGCACTTCAA GGTGAAGTGG CTCCACAATG AATATGTGCG CGATCTGCCT 
GCCCTCCAGG GGCAAGTGCC TGAGTACCCA GCGTGGTTTG AGCAGTTTGT GCTACAGTGG 
CTAGATGAGA ATGAAGATGT GTCACTGGAA TTCCTTCGTG GGGCCTTGGA ACGAGATAAG 
AAGGATGGGT TCCAGCAGAC ATCAGAGCAT GCCTTGTTTT CCTGTTCTGT GGTGGATGTC 
TTTACACAAC TCAACCAGAG CTTTGAGATC ATCCGGAAGT TGGAATGTCC AGACCCCAAC 
ATCCTTGCCC ACTACATGAG AAGGTTTGCT AAGACCATTG GGAAAGTGCT GATGCAGTAT 
GCAGACATCT TATCAAAGAA CTTCCCAGCT TACTGCACAA AGGAGAGACT GCCCTGTATC 
CTGATGAACA ACATGCAGCA ACTGAGGGTC CAGCTGGAGA AAATGTTTGA GGCCATGGGA 
GGCAAAGAGC TGGACTCCGA AGCTGCAGAC AGTCTGAAGG AGCTGCAGGT GAAACTGAAT 
ACAGTTCTGG ATGAGCTTAG CATGGTGTTT GGAAACAGCT TCCAGGTGCG GATTGATGAA 
TGTGTTCGAC AAATGGCTGA CATCCTGGGC CAAGTTCGGG GCACAGGGAA TGCATCCCCT 
AACGCCAGGG CCTCGGTGGC TCAGGATGCA GATAGTGTGC TGCGACCTCT CATGGACTTC 
CTGGATGGCA ACCTCACGCT GTTTGCCACT GTGTGTGAGA AGACAGTTCT GAAGCGGGTC 
CTGAAGGAGC TCTGGCGGGT GGTGATGAAC ACCATGGAGC GGGTTATTGT CCTGCCCCCA 
CTCACTGACC AGACGGGCAC CCAGCTGATC TTAACTGCTG CCAAGGAGCT GAGCCAGCTT 
TCCAAACTCA AGGACCACAT GGTACGGGAG GAAACACGGA ATCTCACTCC AAAGCAGTGT 
GCTGTCCTCG ACTTGGCCTT GGACACCATC AAGCAATACT TCCATGCAGG AGGCAATGGT 
CTGAAGAAAA CCTTCCTGGA AAAGAGCCCA GACCTGCAGT CTCTGCGTTA TGCCCTCTCT 
CTGTATACAC AGACTACAGA CACTCTCATC AAGACATTTG TGCGCTCACA GACTGCCCAG 
GTACATGATG GGAAAGGTAT TAGGTTTACT GCTAACGAGG ACATTCGGCC AGAAAAGGGG 
GCTGGTGTGG ATGACCCCGT GGGAGAAGTC TCTATTCAGG TGGACTTATT TACACATCCT 
GGCACTGGGG AGCATAAGGT CACAGTGAAA GTGGTAGCAG CCAATGACCT CAAGTGGCAG 
ACAGCGGGTA TGTTCCGGCC TTTTGTGGAA GTGACCATGG TTGGCCCACA CCAAAGTGAT 
AAGAAGAGGA AGTTCACAAC CAAGTCTAAA AGTAACAACT GGACTCCCAA GTACAACGAG 
ACGTTTCACT TCCTCCTGGG AAATGAAGAG GGTCCAGAGG CCTATGAATT GCAGATCTGT 
GTGAAGGATT ACTGCTTTGC CCGGGAGGAT CGTGTGATAG GACTGGCAGT GATGCCCCTG 
AGGGATGTGG CAGCTAAGGG CAGCTGTGCC TGCTGGTGCC CACTGGGCCG GAAGATCCAT 
ATGGATGAGA CAGGCATGAC CATTCTCCGG ATTCTGTCTC AGAGGAGCAA TGACGAGGTA 
GCCCGAGAGT TCGTGAAGCT CAAATCAGAG TCTCGATCCA CAGAGGAAGG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( NM_001081413.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074882.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant 2, mRNA.

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CloneID	OMu02383
Clone ID Related Accession (Same CDS sequence)	NM_001081413.2
Accession Version	NM_001081413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY741509.1, AL672276.9, BB612447.1, CK636958.1, CD351898.1, CO434486.1, CB520764.1, CN459646.1, CB245273.1, BU613335.1, CX568667.1, BQ572350.1, EL605839.1, CJ053228.1, DV661336.1, BP758729.1 and BI202627.1. On Jul 18, 2015 this sequence version replaced NM_001081413.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region compared to variant 1. The encoded isoform (2) has the same N- and C-termini, but is one amino acid shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC158025.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGAGAC 
AGTGACTACC GCAGTGAGAC CAGCAATAGC GCCCCACCTC CTTACCACAC GACTACCCAG 
CCCAACGCTT CTGTACACCA GTTCCCTGTT CCTGTGCGGC TGCCACAGCA GCTCTTTCTT 
CAAGGCAGTT CCCATGACTC TTGCAATGAC TCTATGCAGA GTTACGACCT TGATTATCCA 
GAGCGTCGGG CCCTCAGCCC TACCAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CAAGGAAGCT CTCTGCTGAG TGAGCTGGAC CAGTATCATG AGCAAGATGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGTCATAGC TATGGCAGCC TCTCCAAAGA TGGCCAGGCT 
GGTTTGGGAG AACAAGAGAA AGCCTTGGAA GTGACATGTG AATCAGAAAA AGAGAAAACA 
GGTGAATCCA AGGAGATGAG AGATGATGCT ACAATCCACC CTCCTTCAGA TCTGGTGCTG 
CATAAGGACC ATGTCCTAGG ACCCCAGGAG AGTCTTCCTG AGGAGACCGC ATCATCGCCA 
TTTACCCAGG CCAGAGCACA CTGGTTCCGA GCGGTTACCA AGGTCCGACT CCAACTGCAG 
GAGATTTCAG ATGATGGTGA CCCCTCCTTG CCTCAGTGGC TCCCAGAAGG GCCAGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGAAGGA AGAAGCCACT GCCACTTGTC 
AGTGATCTGT CACTGGTCCA GTCACGGAAA GCTGGGATTA CATCTGCTAT GGCTACACGT 
ACCTCTCTCA AGGATGAAGA ACTGAAATCT CATGTGTATA AGAAAACTCT GCAGGCCTTA 
ATCTACCCCA TCTCGTGTAC CACGCCCCAC AACTTTGAGG TCTGGTCTGC CACTACACCT 
ACCTATTGCT ATGAGTGTGA AGGCTTGCTC TGGGGCCTTG CCCGGCAAGG CATGCGCTGC 
AGCGAGTGTG GAGTCAAGTG CCATGAGAAG TGCCAGGACC TGCTCAATGC TGACTGCCTA 
CAGCGGGCTG CTGAAAAGAG CTCTAAGCAT GGAGCTGAGG ACCGAACTCA GAACATTATC 
ATGGCCATGA AGGACCGTAT GAAGATCCGA GAACGGAATA AACCAGAGAT CTTTGAGGTT 
ATCCGGGATG TGTTCACAGT GAGCAAAGTT GCGCATGTGC AGCAGATGAA AACAGTAAAG 
CAGAGTGTCC TGGATGGCAC CTCCAAGTGG TCAGCCAAAA TTACCATCAC GGTGGTTTGT 
GCCCAGGGCC TACAAGCCAA AGACAAGACA GGATCCAGTG ACCCTTATGT GACTGTGCAA 
GTTGGGAAAA CCAAGAAGCG CACCAAGACC ATTTTTGGAA ATTTGAATCC TGTTTGGGAA 
GAGAAGTTCC ATTTTGAGTG CCACAACTCT TCTGATCGAA TTAAGGTACG CGTGTGGGAT 
GAGGATGATG ACATCAAGTC CAGAGTAAAG CAGCGGCTAA AGCGAGAGTC AGATGATTTC 
CTTGGCCAAA CTATCATTGA AGTTCGAACG CTGAGTGGAG AGATGGATGT CTGGTACAAT 
CTGGAGAAGA GAACAGACAA GTCAGCTGTC TCAGGGGCTA TCCGACTTCA AATCAGTGTG 
GAGATCAAGG GGGAGGAGAA GGTAGCCCCA TACCATGTAC AATATACATG TCTCCATGAG 
AACCTTTTCC ATTACCTCAC AGACATTCAG GGCAGTGGAG GAGTCTGGAT CCCAGAGGCT 
CGGGGGGACG ATGCATGGAA GGTGTACTTT GATGAGACAG CCCAAGAAAT TGTGGATGAA 
TTCGCTATGC GCTATGGCAT CGAGTCCATT TATCAGGCCA TGACGCACTT TGCATGTTTG 
TCATCCAAGT ACATGTGTCC TGGTGTACCA GCCGTGATGA GCACCTTACT GGCCAACATC 
AATGCCTATT ATGCTCACAC AACTGCCTCC ACTAACGTCT CTGCATCTGA TCGTTTTGCA 
GCCTCCAACT TTGGTAAAGA AAGATTTGTA AAACTGCTGG ACCAGCTACA CAACTCACTG 
AGGATAGACC TCTCTACATA CAGGAATAAC TTTCCTGCTG GGAGCCCTGA GCGGCTTCAG 
GATTTAAAGT CTACAGTGGA TTTGCTGACC AGCATTACTT TCTTCAGGAT GAAGGTGCAA 
GAACTTCAGA GCCCTCCAAG AGCCAGCCAG GTGGTAAAGG ACTGTGTGAA AGCCTGCTTG 
AACTCTACAT ATGAATACAT CTTCAACAAC TGCCATGACC TCTACAGTCA CCAGTATCAG 
CTGGAACAAC CTCTAGAGGA ACCAGGGCCC AGCATTCGGA ACTTGGATTT CTGGCCCAAA 
CTTATCACAC TCATTGTTTC GATCATAGAG GAGGATAAGA ATTCCTACAC ACCTGTTCTG 
AGCCAGTTTC CTCAGGAGTT GAACGTAGGA AAAGTCAGTG CAGAAGTGAT GTGGCATCTG 
TTTGCTCAAG ACATGAAATA TGCACTGGAG GAACACGAGA AAGACCGGCT GTGTAAGAGT 
GCTGACTACA TGAACCTGCA CTTCAAGGTG AAGTGGCTCC ACAATGAATA TGTGCGCGAT 
CTGCCTGCCC TCCAGGGGCA AGTGCCTGAG TACCCAGCGT GGTTTGAGCA GTTTGTGCTA 
CAGTGGCTAG ATGAGAATGA AGATGTGTCA CTGGAATTCC TTCGTGGGGC CTTGGAACGA 
GATAAGAAGG ATGGGTTCCA GCAGACATCA GAGCATGCCT TGTTTTCCTG TTCTGTGGTG 
GATGTCTTTA CACAACTCAA CCAGAGCTTT GAGATCATCC GGAAGTTGGA ATGTCCAGAC 
CCCAACATCC TTGCCCACTA CATGAGAAGG TTTGCTAAGA CCATTGGGAA AGTGCTGATG 
CAGTATGCAG ACATCTTATC AAAGAACTTC CCAGCTTACT GCACAAAGGA GAGACTGCCC 
TGTATCCTGA TGAACAACAT GCAGCAACTG AGGGTCCAGC TGGAGAAAAT GTTTGAGGCC 
ATGGGAGGCA AAGAGCTGGA CTCCGAAGCT GCAGACAGTC TGAAGGAGCT GCAGGTGAAA 
CTGAATACAG TTCTGGATGA GCTTAGCATG GTGTTTGGAA ACAGCTTCCA GGTGCGGATT 
GATGAATGTG TTCGACAAAT GGCTGACATC CTGGGCCAAG TTCGGGGCAC AGGGAATGCA 
TCCCCTAACG CCAGGGCCTC GGTGGCTCAG GATGCAGATA GTGTGCTGCG ACCTCTCATG 
GACTTCCTGG ATGGCAACCT CACGCTGTTT GCCACTGTGT GTGAGAAGAC AGTTCTGAAG 
CGGGTCCTGA AGGAGCTCTG GCGGGTGGTG ATGAACACCA TGGAGCGGGT TATTGTCCTG 
CCCCCACTCA CTGACCAGAC GGGCACCCAG CTGATCTTAA CTGCTGCCAA GGAGCTGAGC 
CAGCTTTCCA AACTCAAGGA CCACATGGTA CGGGAGGAAA CACGGAATCT CACTCCAAAG 
CAGTGTGCTG TCCTCGACTT GGCCTTGGAC ACCATCAAGC AATACTTCCA TGCAGGAGGC 
AATGGTCTGA AGAAAACCTT CCTGGAAAAG AGCCCAGACC TGCAGTCTCT GCGTTATGCC 
CTCTCTCTGT ATACACAGAC TACAGACACT CTCATCAAGA CATTTGTGCG CTCACAGACT 
GCCCAGGGGG CTGGTGTGGA TGACCCCGTG GGAGAAGTCT CTATTCAGGT GGACTTATTT 
ACACATCCTG GCACTGGGGA GCATAAGGTC ACAGTGAAAG TGGTAGCAGC CAATGACCTC 
AAGTGGCAGA CAGCGGGTAT GTTCCGGCCT TTTGTGGAAG TGACCATGGT TGGCCCACAC 
CAAAGTGATA AGAAGAGGAA GTTCACAACC AAGTCTAAAA GTAACAACTG GACTCCCAAG 
TACAACGAGA CGTTTCACTT CCTCCTGGGA AATGAAGAGG GTCCAGAGGC CTATGAATTG 
CAGATCTGTG TGAAGGATTA CTGCTTTGCC CGGGAGGATC GTGTGATAGG ACTGGCAGTG 
ATGCCCCTGA GGGATGTGGC AGCTAAGGGC AGCTGTGCCT GCTGGTGCCC ACTGGGCCGG 
AAGATCCATA TGGATGAGAC AGGCATGACC ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT 
GACGAGGTAG CCCGAGAGTT CGTGAAGCTC AAATCAGAGT CTCGATCCAC AGAGGAAGGG 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074882.1
CDS	312..5117
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCCHWTYLGWGEHQTFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	NM_001081413.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081413.2

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGAGAC 
AGTGACTACC GCAGTGAGAC CAGCAATAGC GCCCCACCTC CTTACCACAC GACTACCCAG 
CCCAACGCTT CTGTACACCA GTTCCCTGTT CCTGTGCGGC TGCCACAGCA GCTCTTTCTT 
CAAGGCAGTT CCCATGACTC TTGCAATGAC TCTATGCAGA GTTACGACCT TGATTATCCA 
GAGCGTCGGG CCCTCAGCCC TACCAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CAAGGAAGCT CTCTGCTGAG TGAGCTGGAC CAGTATCATG AGCAAGATGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGTCATAGC TATGGCAGCC TCTCCAAAGA TGGCCAGGCT 
GGTTTGGGAG AACAAGAGAA AGCCTTGGAA GTGACATGTG AATCAGAAAA AGAGAAAACA 
GGTGAATCCA AGGAGATGAG AGATGATGCT ACAATCCACC CTCCTTCAGA TCTGGTGCTG 
CATAAGGACC ATGTCCTAGG ACCCCAGGAG AGTCTTCCTG AGGAGACCGC ATCATCGCCA 
TTTACCCAGG CCAGAGCACA CTGGTTCCGA GCGGTTACCA AGGTCCGACT CCAACTGCAG 
GAGATTTCAG ATGATGGTGA CCCCTCCTTG CCTCAGTGGC TCCCAGAAGG GCCAGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGAAGGA AGAAGCCACT GCCACTTGTC 
AGTGATCTGT CACTGGTCCA GTCACGGAAA GCTGGGATTA CATCTGCTAT GGCTACACGT 
ACCTCTCTCA AGGATGAAGA ACTGAAATCT CATGTGTATA AGAAAACTCT GCAGGCCTTA 
ATCTACCCCA TCTCGTGTAC CACGCCCCAC AACTTTGAGG TCTGGTCTGC CACTACACCT 
ACCTATTGCT ATGAGTGTGA AGGCTTGCTC TGGGGCCTTG CCCGGCAAGG CATGCGCTGC 
AGCGAGTGTG GAGTCAAGTG CCATGAGAAG TGCCAGGACC TGCTCAATGC TGACTGCCTA 
CAGCGGGCTG CTGAAAAGAG CTCTAAGCAT GGAGCTGAGG ACCGAACTCA GAACATTATC 
ATGGCCATGA AGGACCGTAT GAAGATCCGA GAACGGAATA AACCAGAGAT CTTTGAGGTT 
ATCCGGGATG TGTTCACAGT GAGCAAAGTT GCGCATGTGC AGCAGATGAA AACAGTAAAG 
CAGAGTGTCC TGGATGGCAC CTCCAAGTGG TCAGCCAAAA TTACCATCAC GGTGGTTTGT 
GCCCAGGGCC TACAAGCCAA AGACAAGACA GGATCCAGTG ACCCTTATGT GACTGTGCAA 
GTTGGGAAAA CCAAGAAGCG CACCAAGACC ATTTTTGGAA ATTTGAATCC TGTTTGGGAA 
GAGAAGTTCC ATTTTGAGTG CCACAACTCT TCTGATCGAA TTAAGGTACG CGTGTGGGAT 
GAGGATGATG ACATCAAGTC CAGAGTAAAG CAGCGGCTAA AGCGAGAGTC AGATGATTTC 
CTTGGCCAAA CTATCATTGA AGTTCGAACG CTGAGTGGAG AGATGGATGT CTGGTACAAT 
CTGGAGAAGA GAACAGACAA GTCAGCTGTC TCAGGGGCTA TCCGACTTCA AATCAGTGTG 
GAGATCAAGG GGGAGGAGAA GGTAGCCCCA TACCATGTAC AATATACATG TCTCCATGAG 
AACCTTTTCC ATTACCTCAC AGACATTCAG GGCAGTGGAG GAGTCTGGAT CCCAGAGGCT 
CGGGGGGACG ATGCATGGAA GGTGTACTTT GATGAGACAG CCCAAGAAAT TGTGGATGAA 
TTCGCTATGC GCTATGGCAT CGAGTCCATT TATCAGGCCA TGACGCACTT TGCATGTTTG 
TCATCCAAGT ACATGTGTCC TGGTGTACCA GCCGTGATGA GCACCTTACT GGCCAACATC 
AATGCCTATT ATGCTCACAC AACTGCCTCC ACTAACGTCT CTGCATCTGA TCGTTTTGCA 
GCCTCCAACT TTGGTAAAGA AAGATTTGTA AAACTGCTGG ACCAGCTACA CAACTCACTG 
AGGATAGACC TCTCTACATA CAGGAATAAC TTTCCTGCTG GGAGCCCTGA GCGGCTTCAG 
GATTTAAAGT CTACAGTGGA TTTGCTGACC AGCATTACTT TCTTCAGGAT GAAGGTGCAA 
GAACTTCAGA GCCCTCCAAG AGCCAGCCAG GTGGTAAAGG ACTGTGTGAA AGCCTGCTTG 
AACTCTACAT ATGAATACAT CTTCAACAAC TGCCATGACC TCTACAGTCA CCAGTATCAG 
CTGGAACAAC CTCTAGAGGA ACCAGGGCCC AGCATTCGGA ACTTGGATTT CTGGCCCAAA 
CTTATCACAC TCATTGTTTC GATCATAGAG GAGGATAAGA ATTCCTACAC ACCTGTTCTG 
AGCCAGTTTC CTCAGGAGTT GAACGTAGGA AAAGTCAGTG CAGAAGTGAT GTGGCATCTG 
TTTGCTCAAG ACATGAAATA TGCACTGGAG GAACACGAGA AAGACCGGCT GTGTAAGAGT 
GCTGACTACA TGAACCTGCA CTTCAAGGTG AAGTGGCTCC ACAATGAATA TGTGCGCGAT 
CTGCCTGCCC TCCAGGGGCA AGTGCCTGAG TACCCAGCGT GGTTTGAGCA GTTTGTGCTA 
CAGTGGCTAG ATGAGAATGA AGATGTGTCA CTGGAATTCC TTCGTGGGGC CTTGGAACGA 
GATAAGAAGG ATGGGTTCCA GCAGACATCA GAGCATGCCT TGTTTTCCTG TTCTGTGGTG 
GATGTCTTTA CACAACTCAA CCAGAGCTTT GAGATCATCC GGAAGTTGGA ATGTCCAGAC 
CCCAACATCC TTGCCCACTA CATGAGAAGG TTTGCTAAGA CCATTGGGAA AGTGCTGATG 
CAGTATGCAG ACATCTTATC AAAGAACTTC CCAGCTTACT GCACAAAGGA GAGACTGCCC 
TGTATCCTGA TGAACAACAT GCAGCAACTG AGGGTCCAGC TGGAGAAAAT GTTTGAGGCC 
ATGGGAGGCA AAGAGCTGGA CTCCGAAGCT GCAGACAGTC TGAAGGAGCT GCAGGTGAAA 
CTGAATACAG TTCTGGATGA GCTTAGCATG GTGTTTGGAA ACAGCTTCCA GGTGCGGATT 
GATGAATGTG TTCGACAAAT GGCTGACATC CTGGGCCAAG TTCGGGGCAC AGGGAATGCA 
TCCCCTAACG CCAGGGCCTC GGTGGCTCAG GATGCAGATA GTGTGCTGCG ACCTCTCATG 
GACTTCCTGG ATGGCAACCT CACGCTGTTT GCCACTGTGT GTGAGAAGAC AGTTCTGAAG 
CGGGTCCTGA AGGAGCTCTG GCGGGTGGTG ATGAACACCA TGGAGCGGGT TATTGTCCTG 
CCCCCACTCA CTGACCAGAC GGGCACCCAG CTGATCTTAA CTGCTGCCAA GGAGCTGAGC 
CAGCTTTCCA AACTCAAGGA CCACATGGTA CGGGAGGAAA CACGGAATCT CACTCCAAAG 
CAGTGTGCTG TCCTCGACTT GGCCTTGGAC ACCATCAAGC AATACTTCCA TGCAGGAGGC 
AATGGTCTGA AGAAAACCTT CCTGGAAAAG AGCCCAGACC TGCAGTCTCT GCGTTATGCC 
CTCTCTCTGT ATACACAGAC TACAGACACT CTCATCAAGA CATTTGTGCG CTCACAGACT 
GCCCAGGGGG CTGGTGTGGA TGACCCCGTG GGAGAAGTCT CTATTCAGGT GGACTTATTT 
ACACATCCTG GCACTGGGGA GCATAAGGTC ACAGTGAAAG TGGTAGCAGC CAATGACCTC 
AAGTGGCAGA CAGCGGGTAT GTTCCGGCCT TTTGTGGAAG TGACCATGGT TGGCCCACAC 
CAAAGTGATA AGAAGAGGAA GTTCACAACC AAGTCTAAAA GTAACAACTG GACTCCCAAG 
TACAACGAGA CGTTTCACTT CCTCCTGGGA AATGAAGAGG GTCCAGAGGC CTATGAATTG 
CAGATCTGTG TGAAGGATTA CTGCTTTGCC CGGGAGGATC GTGTGATAGG ACTGGCAGTG 
ATGCCCCTGA GGGATGTGGC AGCTAAGGGC AGCTGTGCCT GCTGGTGCCC ACTGGGCCGG 
AAGATCCATA TGGATGAGAC AGGCATGACC ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT 
GACGAGGTAG CCCGAGAGTT CGTGAAGCTC AAATCAGAGT CTCGATCCAC AGAGGAAGGG 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( NM_021468.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067443.2 Mus musculus unc-13 homolog B (Unc13b), transcript variant 1, mRNA.

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CloneID	OMu12393
Clone ID Related Accession (Same CDS sequence)	NM_021468.3
Accession Version	NM_021468.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY741509.1, AL672276.9, BB612447.1, CK636958.1, CD351898.1, CO434486.1, CB520764.1, CN459646.1, BU613335.1, CX568667.1, BQ572350.1, EL605839.1, CJ053228.1, DV661336.1, BP758729.1 and BI202627.1. On Jul 18, 2015 this sequence version replaced NM_021468.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC157967.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGAGAC 
AGTGACTACC GCAGTGAGAC CAGCAATAGC GCCCCACCTC CTTACCACAC GACTACCCAG 
CCCAACGCTT CTGTACACCA GTTCCCTGTT CCTGTGCGGC TGCCACAGCA GCTCTTTCTT 
CAAGGCAGTT CCCATGACTC TTGCAATGAC TCTATGCAGA GTTACGACCT TGATTATCCA 
GAGCGTCGGG CCCTCAGCCC TACCAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CAAGGAAGCT CTCTGCTGAG TGAGCTGGAC CAGTATCATG AGCAAGATGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGTCATAGC TATGGCAGCC TCTCCAAAGA TGGCCAGGCT 
GGTTTGGGAG AACAAGAGAA AGCCTTGGAA GTGACATGTG AATCAGAAAA AGAGAAAACA 
GGTGAATCCA AGGAGATGAG AGATGATGCT ACAATCCACC CTCCTTCAGA TCTGGTGCTG 
CATAAGGACC ATGTCCTAGG ACCCCAGGAG AGTCTTCCTG AGGAGACCGC ATCATCGCCA 
TTTACCCAGG CCAGAGCACA CTGGTTCCGA GCGGTTACCA AGGTCCGACT CCAACTGCAG 
GAGATTTCAG ATGATGGTGA CCCCTCCTTG CCTCAGTGGC TCCCAGAAGG GCCAGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGAAGGA AGAAGCCACT GCCACTTGTC 
AGTGATCTGT CACTGGTCCA GTCACGGAAA GCTGGGATTA CATCTGCTAT GGCTACACGT 
ACCTCTCTCA AGGATGAAGA ACTGAAATCT CATGTGTATA AGAAAACTCT GCAGGCCTTA 
ATCTACCCCA TCTCGTGTAC CACGCCCCAC AACTTTGAGG TCTGGTCTGC CACTACACCT 
ACCTATTGCT ATGAGTGTGA AGGCTTGCTC TGGGGCCTTG CCCGGCAAGG CATGCGCTGC 
AGCGAGTGTG GAGTCAAGTG CCATGAGAAG TGCCAGGACC TGCTCAATGC TGACTGCCTA 
CAGCGGGCTG CTGAAAAGAG CTCTAAGCAT GGAGCTGAGG ACCGAACTCA GAACATTATC 
ATGGCCATGA AGGACCGTAT GAAGATCCGA GAACGGAATA AACCAGAGAT CTTTGAGGTT 
ATCCGGGATG TGTTCACAGT GAGCAAAGTT GCGCATGTGC AGCAGATGAA AACAGTAAAG 
CAGAGTGTCC TGGATGGCAC CTCCAAGTGG TCAGCCAAAA TTACCATCAC GGTGGTTTGT 
GCCCAGGGCC TACAAGCCAA AGACAAGACA GGATCCAGTG ACCCTTATGT GACTGTGCAA 
GTTGGGAAAA CCAAGAAGCG CACCAAGACC ATTTTTGGAA ATTTGAATCC TGTTTGGGAA 
GAGAAGTTCC ATTTTGAGTG CCACAACTCT TCTGATCGAA TTAAGGTACG CGTGTGGGAT 
GAGGATGATG ACATCAAGTC CAGAGTAAAG CAGCGGCTAA AGCGAGAGTC AGATGATTTC 
CTTGGCCAAA CTATCATTGA AGTTCGAACG CTGAGTGGAG AGATGGATGT CTGGTACAAT 
CTGGAGAAGA GAACAGACAA GTCAGCTGTC TCAGGGGCTA TCCGACTTCA AATCAGTGTG 
GAGATCAAGG GGGAGGAGAA GGTAGCCCCA TACCATGTAC AATATACATG TCTCCATGAG 
AACCTTTTCC ATTACCTCAC AGACATTCAG GGCAGTGGAG GAGTCTGGAT CCCAGAGGCT 
CGGGGGGACG ATGCATGGAA GGTGTACTTT GATGAGACAG CCCAAGAAAT TGTGGATGAA 
TTCGCTATGC GCTATGGCAT CGAGTCCATT TATCAGGCCA TGACGCACTT TGCATGTTTG 
TCATCCAAGT ACATGTGTCC TGGTGTACCA GCCGTGATGA GCACCTTACT GGCCAACATC 
AATGCCTATT ATGCTCACAC AACTGCCTCC ACTAACGTCT CTGCATCTGA TCGTTTTGCA 
GCCTCCAACT TTGGTAAAGA AAGATTTGTA AAACTGCTGG ACCAGCTACA CAACTCACTG 
AGGATAGACC TCTCTACATA CAGGAATAAC TTTCCTGCTG GGAGCCCTGA GCGGCTTCAG 
GATTTAAAGT CTACAGTGGA TTTGCTGACC AGCATTACTT TCTTCAGGAT GAAGGTGCAA 
GAACTTCAGA GCCCTCCAAG AGCCAGCCAG GTGGTAAAGG ACTGTGTGAA AGCCTGCTTG 
AACTCTACAT ATGAATACAT CTTCAACAAC TGCCATGACC TCTACAGTCA CCAGTATCAG 
CTGCAGGAAC AACCTCTAGA GGAACCAGGG CCCAGCATTC GGAACTTGGA TTTCTGGCCC 
AAACTTATCA CACTCATTGT TTCGATCATA GAGGAGGATA AGAATTCCTA CACACCTGTT 
CTGAGCCAGT TTCCTCAGGA GTTGAACGTA GGAAAAGTCA GTGCAGAAGT GATGTGGCAT 
CTGTTTGCTC AAGACATGAA ATATGCACTG GAGGAACACG AGAAAGACCG GCTGTGTAAG 
AGTGCTGACT ACATGAACCT GCACTTCAAG GTGAAGTGGC TCCACAATGA ATATGTGCGC 
GATCTGCCTG CCCTCCAGGG GCAAGTGCCT GAGTACCCAG CGTGGTTTGA GCAGTTTGTG 
CTACAGTGGC TAGATGAGAA TGAAGATGTG TCACTGGAAT TCCTTCGTGG GGCCTTGGAA 
CGAGATAAGA AGGATGGGTT CCAGCAGACA TCAGAGCATG CCTTGTTTTC CTGTTCTGTG 
GTGGATGTCT TTACACAACT CAACCAGAGC TTTGAGATCA TCCGGAAGTT GGAATGTCCA 
GACCCCAACA TCCTTGCCCA CTACATGAGA AGGTTTGCTA AGACCATTGG GAAAGTGCTG 
ATGCAGTATG CAGACATCTT ATCAAAGAAC TTCCCAGCTT ACTGCACAAA GGAGAGACTG 
CCCTGTATCC TGATGAACAA CATGCAGCAA CTGAGGGTCC AGCTGGAGAA AATGTTTGAG 
GCCATGGGAG GCAAAGAGCT GGACTCCGAA GCTGCAGACA GTCTGAAGGA GCTGCAGGTG 
AAACTGAATA CAGTTCTGGA TGAGCTTAGC ATGGTGTTTG GAAACAGCTT CCAGGTGCGG 
ATTGATGAAT GTGTTCGACA AATGGCTGAC ATCCTGGGCC AAGTTCGGGG CACAGGGAAT 
GCATCCCCTA ACGCCAGGGC CTCGGTGGCT CAGGATGCAG ATAGTGTGCT GCGACCTCTC 
ATGGACTTCC TGGATGGCAA CCTCACGCTG TTTGCCACTG TGTGTGAGAA GACAGTTCTG 
AAGCGGGTCC TGAAGGAGCT CTGGCGGGTG GTGATGAACA CCATGGAGCG GGTTATTGTC 
CTGCCCCCAC TCACTGACCA GACGGGCACC CAGCTGATCT TAACTGCTGC CAAGGAGCTG 
AGCCAGCTTT CCAAACTCAA GGACCACATG GTACGGGAGG AAACACGGAA TCTCACTCCA 
AAGCAGTGTG CTGTCCTCGA CTTGGCCTTG GACACCATCA AGCAATACTT CCATGCAGGA 
GGCAATGGTC TGAAGAAAAC CTTCCTGGAA AAGAGCCCAG ACCTGCAGTC TCTGCGTTAT 
GCCCTCTCTC TGTATACACA GACTACAGAC ACTCTCATCA AGACATTTGT GCGCTCACAG 
ACTGCCCAGG GGGCTGGTGT GGATGACCCC GTGGGAGAAG TCTCTATTCA GGTGGACTTA 
TTTACACATC CTGGCACTGG GGAGCATAAG GTCACAGTGA AAGTGGTAGC AGCCAATGAC 
CTCAAGTGGC AGACAGCGGG TATGTTCCGG CCTTTTGTGG AAGTGACCAT GGTTGGCCCA 
CACCAAAGTG ATAAGAAGAG GAAGTTCACA ACCAAGTCTA AAAGTAACAA CTGGACTCCC 
AAGTACAACG AGACGTTTCA CTTCCTCCTG GGAAATGAAG AGGGTCCAGA GGCCTATGAA 
TTGCAGATCT GTGTGAAGGA TTACTGCTTT GCCCGGGAGG ATCGTGTGAT AGGACTGGCA 
GTGATGCCCC TGAGGGATGT GGCAGCTAAG GGCAGCTGTG CCTGCTGGTG CCCACTGGGC 
CGGAAGATCC ATATGGATGA GACAGGCATG ACCATTCTCC GGATTCTGTC TCAGAGGAGC 
AATGACGAGG TAGCCCGAGA GTTCGTGAAG CTCAAATCAG AGTCTCGATC CACAGAGGAA 
GGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067443.2
CDS	312..5120
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCCHWTYLGWGEHQTFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	NM_021468.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021468.3

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGAGAC 
AGTGACTACC GCAGTGAGAC CAGCAATAGC GCCCCACCTC CTTACCACAC GACTACCCAG 
CCCAACGCTT CTGTACACCA GTTCCCTGTT CCTGTGCGGC TGCCACAGCA GCTCTTTCTT 
CAAGGCAGTT CCCATGACTC TTGCAATGAC TCTATGCAGA GTTACGACCT TGATTATCCA 
GAGCGTCGGG CCCTCAGCCC TACCAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CAAGGAAGCT CTCTGCTGAG TGAGCTGGAC CAGTATCATG AGCAAGATGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGTCATAGC TATGGCAGCC TCTCCAAAGA TGGCCAGGCT 
GGTTTGGGAG AACAAGAGAA AGCCTTGGAA GTGACATGTG AATCAGAAAA AGAGAAAACA 
GGTGAATCCA AGGAGATGAG AGATGATGCT ACAATCCACC CTCCTTCAGA TCTGGTGCTG 
CATAAGGACC ATGTCCTAGG ACCCCAGGAG AGTCTTCCTG AGGAGACCGC ATCATCGCCA 
TTTACCCAGG CCAGAGCACA CTGGTTCCGA GCGGTTACCA AGGTCCGACT CCAACTGCAG 
GAGATTTCAG ATGATGGTGA CCCCTCCTTG CCTCAGTGGC TCCCAGAAGG GCCAGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGAAGGA AGAAGCCACT GCCACTTGTC 
AGTGATCTGT CACTGGTCCA GTCACGGAAA GCTGGGATTA CATCTGCTAT GGCTACACGT 
ACCTCTCTCA AGGATGAAGA ACTGAAATCT CATGTGTATA AGAAAACTCT GCAGGCCTTA 
ATCTACCCCA TCTCGTGTAC CACGCCCCAC AACTTTGAGG TCTGGTCTGC CACTACACCT 
ACCTATTGCT ATGAGTGTGA AGGCTTGCTC TGGGGCCTTG CCCGGCAAGG CATGCGCTGC 
AGCGAGTGTG GAGTCAAGTG CCATGAGAAG TGCCAGGACC TGCTCAATGC TGACTGCCTA 
CAGCGGGCTG CTGAAAAGAG CTCTAAGCAT GGAGCTGAGG ACCGAACTCA GAACATTATC 
ATGGCCATGA AGGACCGTAT GAAGATCCGA GAACGGAATA AACCAGAGAT CTTTGAGGTT 
ATCCGGGATG TGTTCACAGT GAGCAAAGTT GCGCATGTGC AGCAGATGAA AACAGTAAAG 
CAGAGTGTCC TGGATGGCAC CTCCAAGTGG TCAGCCAAAA TTACCATCAC GGTGGTTTGT 
GCCCAGGGCC TACAAGCCAA AGACAAGACA GGATCCAGTG ACCCTTATGT GACTGTGCAA 
GTTGGGAAAA CCAAGAAGCG CACCAAGACC ATTTTTGGAA ATTTGAATCC TGTTTGGGAA 
GAGAAGTTCC ATTTTGAGTG CCACAACTCT TCTGATCGAA TTAAGGTACG CGTGTGGGAT 
GAGGATGATG ACATCAAGTC CAGAGTAAAG CAGCGGCTAA AGCGAGAGTC AGATGATTTC 
CTTGGCCAAA CTATCATTGA AGTTCGAACG CTGAGTGGAG AGATGGATGT CTGGTACAAT 
CTGGAGAAGA GAACAGACAA GTCAGCTGTC TCAGGGGCTA TCCGACTTCA AATCAGTGTG 
GAGATCAAGG GGGAGGAGAA GGTAGCCCCA TACCATGTAC AATATACATG TCTCCATGAG 
AACCTTTTCC ATTACCTCAC AGACATTCAG GGCAGTGGAG GAGTCTGGAT CCCAGAGGCT 
CGGGGGGACG ATGCATGGAA GGTGTACTTT GATGAGACAG CCCAAGAAAT TGTGGATGAA 
TTCGCTATGC GCTATGGCAT CGAGTCCATT TATCAGGCCA TGACGCACTT TGCATGTTTG 
TCATCCAAGT ACATGTGTCC TGGTGTACCA GCCGTGATGA GCACCTTACT GGCCAACATC 
AATGCCTATT ATGCTCACAC AACTGCCTCC ACTAACGTCT CTGCATCTGA TCGTTTTGCA 
GCCTCCAACT TTGGTAAAGA AAGATTTGTA AAACTGCTGG ACCAGCTACA CAACTCACTG 
AGGATAGACC TCTCTACATA CAGGAATAAC TTTCCTGCTG GGAGCCCTGA GCGGCTTCAG 
GATTTAAAGT CTACAGTGGA TTTGCTGACC AGCATTACTT TCTTCAGGAT GAAGGTGCAA 
GAACTTCAGA GCCCTCCAAG AGCCAGCCAG GTGGTAAAGG ACTGTGTGAA AGCCTGCTTG 
AACTCTACAT ATGAATACAT CTTCAACAAC TGCCATGACC TCTACAGTCA CCAGTATCAG 
CTGCAGGAAC AACCTCTAGA GGAACCAGGG CCCAGCATTC GGAACTTGGA TTTCTGGCCC 
AAACTTATCA CACTCATTGT TTCGATCATA GAGGAGGATA AGAATTCCTA CACACCTGTT 
CTGAGCCAGT TTCCTCAGGA GTTGAACGTA GGAAAAGTCA GTGCAGAAGT GATGTGGCAT 
CTGTTTGCTC AAGACATGAA ATATGCACTG GAGGAACACG AGAAAGACCG GCTGTGTAAG 
AGTGCTGACT ACATGAACCT GCACTTCAAG GTGAAGTGGC TCCACAATGA ATATGTGCGC 
GATCTGCCTG CCCTCCAGGG GCAAGTGCCT GAGTACCCAG CGTGGTTTGA GCAGTTTGTG 
CTACAGTGGC TAGATGAGAA TGAAGATGTG TCACTGGAAT TCCTTCGTGG GGCCTTGGAA 
CGAGATAAGA AGGATGGGTT CCAGCAGACA TCAGAGCATG CCTTGTTTTC CTGTTCTGTG 
GTGGATGTCT TTACACAACT CAACCAGAGC TTTGAGATCA TCCGGAAGTT GGAATGTCCA 
GACCCCAACA TCCTTGCCCA CTACATGAGA AGGTTTGCTA AGACCATTGG GAAAGTGCTG 
ATGCAGTATG CAGACATCTT ATCAAAGAAC TTCCCAGCTT ACTGCACAAA GGAGAGACTG 
CCCTGTATCC TGATGAACAA CATGCAGCAA CTGAGGGTCC AGCTGGAGAA AATGTTTGAG 
GCCATGGGAG GCAAAGAGCT GGACTCCGAA GCTGCAGACA GTCTGAAGGA GCTGCAGGTG 
AAACTGAATA CAGTTCTGGA TGAGCTTAGC ATGGTGTTTG GAAACAGCTT CCAGGTGCGG 
ATTGATGAAT GTGTTCGACA AATGGCTGAC ATCCTGGGCC AAGTTCGGGG CACAGGGAAT 
GCATCCCCTA ACGCCAGGGC CTCGGTGGCT CAGGATGCAG ATAGTGTGCT GCGACCTCTC 
ATGGACTTCC TGGATGGCAA CCTCACGCTG TTTGCCACTG TGTGTGAGAA GACAGTTCTG 
AAGCGGGTCC TGAAGGAGCT CTGGCGGGTG GTGATGAACA CCATGGAGCG GGTTATTGTC 
CTGCCCCCAC TCACTGACCA GACGGGCACC CAGCTGATCT TAACTGCTGC CAAGGAGCTG 
AGCCAGCTTT CCAAACTCAA GGACCACATG GTACGGGAGG AAACACGGAA TCTCACTCCA 
AAGCAGTGTG CTGTCCTCGA CTTGGCCTTG GACACCATCA AGCAATACTT CCATGCAGGA 
GGCAATGGTC TGAAGAAAAC CTTCCTGGAA AAGAGCCCAG ACCTGCAGTC TCTGCGTTAT 
GCCCTCTCTC TGTATACACA GACTACAGAC ACTCTCATCA AGACATTTGT GCGCTCACAG 
ACTGCCCAGG GGGCTGGTGT GGATGACCCC GTGGGAGAAG TCTCTATTCA GGTGGACTTA 
TTTACACATC CTGGCACTGG GGAGCATAAG GTCACAGTGA AAGTGGTAGC AGCCAATGAC 
CTCAAGTGGC AGACAGCGGG TATGTTCCGG CCTTTTGTGG AAGTGACCAT GGTTGGCCCA 
CACCAAAGTG ATAAGAAGAG GAAGTTCACA ACCAAGTCTA AAAGTAACAA CTGGACTCCC 
AAGTACAACG AGACGTTTCA CTTCCTCCTG GGAAATGAAG AGGGTCCAGA GGCCTATGAA 
TTGCAGATCT GTGTGAAGGA TTACTGCTTT GCCCGGGAGG ATCGTGTGAT AGGACTGGCA 
GTGATGCCCC TGAGGGATGT GGCAGCTAAG GGCAGCTGTG CCTGCTGGTG CCCACTGGGC 
CGGAAGATCC ATATGGATGA GACAGGCATG ACCATTCTCC GGATTCTGTC TCAGAGGAGC 
AATGACGAGG TAGCCCGAGA GTTCGTGAAG CTCAAATCAG AGTCTCGATC CACAGAGGAA 
GGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( NM_001310758.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297687.1 Mus musculus unc-13 homolog B (Unc13b), transcript variant 3, mRNA.

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CloneID	OMu69012
Clone ID Related Accession (Same CDS sequence)	NM_001310758.1
Accession Version	NM_001310758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY741509.1, AL672276.9, BB612447.1, CK636958.1, CF746404.1, CO434486.1, CB520764.1, CN459646.1, BU613335.1, CX568667.1, BQ572350.1, EL605839.1, CJ053228.1, DV661336.1, BP758729.1 and BI202627.1. On Jul 18, 2015 this sequence version replaced XM_006537796.2. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (3) has the same N- and C-termini, but is shorter and has a distinct amino acid at the splice variant junction compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF115848.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTCCTT TGAAGACCCT 
GATAGTGCCG TTGATGACCG AGACAGTGAC TACCGCAGTG AGACCAGCAA TAGCGCCCCA 
CCTCCTTACC ACACGACTAC CCAGCCCAAC GCTTCTGTAC ACCAGTTCCC TGTTCCTGTG 
CGGCTGCCAC AGCAGCTCTT TCTTCAAGGC AGTTCCCATG ACTCTTGCAA TGACTCTATG 
CAGAGTTACG ACCTTGATTA TCCAGAGCGT CGGGCCCTCA GCCCTACCAG CAGTAGTAGG 
TATGGCTCCT CCTGTAATGT GAGTCAAGGA AGCTCTCTGC TGAGTGAGCT GGACCAGTAT 
CATGAGCAAG ATGATGATGG TCGGGAGAGG GACTCCATTC ATTCTAGTCA TAGCTATGGC 
AGCCTCTCCA AAGATGGCCA GGCTGGTTTG GGAGAACAAG AGAAAGCCTT GGAAGTGACA 
TGTGAATCAG AAAAAGAGAA AACAGGTGAA TCCAAGGAGA TGAGAGATGA TGCTACAATC 
CACCCTCCTT CAGATCTGGT GCTGCATAAG GACCATGTCC TAGGACCCCA GGAGAGTCTT 
CCTGAGGAGA CCGCATCATC GCCATTTACC CAGGCCAGAG CACACTGGTT CCGAGCGGTT 
ACCAAGGTCC GACTCCAACT GCAGGAGATT TCAGATGATG GTGACCCCTC CTTGCCTCAG 
TGGCTCCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGA 
AGGAAGAAGC CACTGCCACT TGTCAGTGAT CTGTCACTGG TCCAGTCACG GAAAGCTGGG 
ATTACATCTG CTATGGCTAC ACGTACCTCT CTCAAGGATG AAGAACTGAA ATCTCATGTG 
TATAAGAAAA CTCTGCAGGC CTTAATCTAC CCCATCTCGT GTACCACGCC CCACAACTTT 
GAGGTCTGGT CTGCCACTAC ACCTACCTAT TGCTATGAGT GTGAAGGCTT GCTCTGGGGC 
CTTGCCCGGC AAGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG 
GACCTGCTCA ATGCTGACTG CCTACAGCGG GCTGCTGAAA AGAGCTCTAA GCATGGAGCT 
GAGGACCGAA CTCAGAACAT TATCATGGCC ATGAAGGACC GTATGAAGAT CCGAGAACGG 
AATAAACCAG AGATCTTTGA GGTTATCCGG GATGTGTTCA CAGTGAGCAA AGTTGCGCAT 
GTGCAGCAGA TGAAAACAGT AAAGCAGAGT GTCCTGGATG GCACCTCCAA GTGGTCAGCC 
AAAATTACCA TCACGGTGGT TTGTGCCCAG GGCCTACAAG CCAAAGACAA GACAGGATCC 
AGTGACCCTT ATGTGACTGT GCAAGTTGGG AAAACCAAGA AGCGCACCAA GACCATTTTT 
GGAAATTTGA ATCCTGTTTG GGAAGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAT 
CGAATTAAGG TACGCGTGTG GGATGAGGAT GATGACATCA AGTCCAGAGT AAAGCAGCGG 
CTAAAGCGAG AGTCAGATGA TTTCCTTGGC CAAACTATCA TTGAAGTTCG AACGCTGAGT 
GGAGAGATGG ATGTCTGGTA CAATCTGGAG AAGAGAACAG ACAAGTCAGC TGTCTCAGGG 
GCTATCCGAC TTCAAATCAG TGTGGAGATC AAGGGGGAGG AGAAGGTAGC CCCATACCAT 
GTACAATATA CATGTCTCCA TGAGAACCTT TTCCATTACC TCACAGACAT TCAGGGCAGT 
GGAGGAGTCT GGATCCCAGA GGCTCGGGGG GACGATGCAT GGAAGGTGTA CTTTGATGAG 
ACAGCCCAAG AAATTGTGGA TGAATTCGCT ATGCGCTATG GCATCGAGTC CATTTATCAG 
GCCATGACGC ACTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT ACCAGCCGTG 
ATGAGCACCT TACTGGCCAA CATCAATGCC TATTATGCTC ACACAACTGC CTCCACTAAC 
GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGTA AAGAAAGATT TGTAAAACTG 
CTGGACCAGC TACACAACTC ACTGAGGATA GACCTCTCTA CATACAGGAA TAACTTTCCT 
GCTGGGAGCC CTGAGCGGCT TCAGGATTTA AAGTCTACAG TGGATTTGCT GACCAGCATT 
ACTTTCTTCA GGATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTGGTA 
AAGGACTGTG TGAAAGCCTG CTTGAACTCT ACATATGAAT ACATCTTCAA CAACTGCCAT 
GACCTCTACA GTCACCAGTA TCAGCTGCAG GAACAACCTC TAGAGGAACC AGGGCCCAGC 
ATTCGGAACT TGGATTTCTG GCCCAAACTT ATCACACTCA TTGTTTCGAT CATAGAGGAG 
GATAAGAATT CCTACACACC TGTTCTGAGC CAGTTTCCTC AGGAGTTGAA CGTAGGAAAA 
GTCAGTGCAG AAGTGATGTG GCATCTGTTT GCTCAAGACA TGAAATATGC ACTGGAGGAA 
CACGAGAAAG ACCGGCTGTG TAAGAGTGCT GACTACATGA ACCTGCACTT CAAGGTGAAG 
TGGCTCCACA ATGAATATGT GCGCGATCTG CCTGCCCTCC AGGGGCAAGT GCCTGAGTAC 
CCAGCGTGGT TTGAGCAGTT TGTGCTACAG TGGCTAGATG AGAATGAAGA TGTGTCACTG 
GAATTCCTTC GTGGGGCCTT GGAACGAGAT AAGAAGGATG GGTTCCAGCA GACATCAGAG 
CATGCCTTGT TTTCCTGTTC TGTGGTGGAT GTCTTTACAC AACTCAACCA GAGCTTTGAG 
ATCATCCGGA AGTTGGAATG TCCAGACCCC AACATCCTTG CCCACTACAT GAGAAGGTTT 
GCTAAGACCA TTGGGAAAGT GCTGATGCAG TATGCAGACA TCTTATCAAA GAACTTCCCA 
GCTTACTGCA CAAAGGAGAG ACTGCCCTGT ATCCTGATGA ACAACATGCA GCAACTGAGG 
GTCCAGCTGG AGAAAATGTT TGAGGCCATG GGAGGCAAAG AGCTGGACTC CGAAGCTGCA 
GACAGTCTGA AGGAGCTGCA GGTGAAACTG AATACAGTTC TGGATGAGCT TAGCATGGTG 
TTTGGAAACA GCTTCCAGGT GCGGATTGAT GAATGTGTTC GACAAATGGC TGACATCCTG 
GGCCAAGTTC GGGGCACAGG GAATGCATCC CCTAACGCCA GGGCCTCGGT GGCTCAGGAT 
GCAGATAGTG TGCTGCGACC TCTCATGGAC TTCCTGGATG GCAACCTCAC GCTGTTTGCC 
ACTGTGTGTG AGAAGACAGT TCTGAAGCGG GTCCTGAAGG AGCTCTGGCG GGTGGTGATG 
AACACCATGG AGCGGGTTAT TGTCCTGCCC CCACTCACTG ACCAGACGGG CACCCAGCTG 
ATCTTAACTG CTGCCAAGGA GCTGAGCCAG CTTTCCAAAC TCAAGGACCA CATGGTACGG 
GAGGAAACAC GGAATCTCAC TCCAAAGCAG TGTGCTGTCC TCGACTTGGC CTTGGACACC 
ATCAAGCAAT ACTTCCATGC AGGAGGCAAT GGTCTGAAGA AAACCTTCCT GGAAAAGAGC 
CCAGACCTGC AGTCTCTGCG TTATGCCCTC TCTCTGTATA CACAGACTAC AGACACTCTC 
ATCAAGACAT TTGTGCGCTC ACAGACTGCC CAGGGGGCTG GTGTGGATGA CCCCGTGGGA 
GAAGTCTCTA TTCAGGTGGA CTTATTTACA CATCCTGGCA CTGGGGAGCA TAAGGTCACA 
GTGAAAGTGG TAGCAGCCAA TGACCTCAAG TGGCAGACAG CGGGTATGTT CCGGCCTTTT 
GTGGAAGTGA CCATGGTTGG CCCACACCAA AGTGATAAGA AGAGGAAGTT CACAACCAAG 
TCTAAAAGTA ACAACTGGAC TCCCAAGTAC AACGAGACGT TTCACTTCCT CCTGGGAAAT 
GAAGAGGGTC CAGAGGCCTA TGAATTGCAG ATCTGTGTGA AGGATTACTG CTTTGCCCGG 
GAGGATCGTG TGATAGGACT GGCAGTGATG CCCCTGAGGG ATGTGGCAGC TAAGGGCAGC 
TGTGCCTGCT GGTGCCCACT GGGCCGGAAG ATCCATATGG ATGAGACAGG CATGACCATT 
CTCCGGATTC TGTCTCAGAG GAGCAATGAC GAGGTAGCCC GAGAGTTCGT GAAGCTCAAA 
TCAGAGTCTC GATCCACAGA GGAAGGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297687.1
CDS	312..5084
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	NM_001310758.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (Unc13b), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310758.1

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTCCTT TGAAGACCCT 
GATAGTGCCG TTGATGACCG AGACAGTGAC TACCGCAGTG AGACCAGCAA TAGCGCCCCA 
CCTCCTTACC ACACGACTAC CCAGCCCAAC GCTTCTGTAC ACCAGTTCCC TGTTCCTGTG 
CGGCTGCCAC AGCAGCTCTT TCTTCAAGGC AGTTCCCATG ACTCTTGCAA TGACTCTATG 
CAGAGTTACG ACCTTGATTA TCCAGAGCGT CGGGCCCTCA GCCCTACCAG CAGTAGTAGG 
TATGGCTCCT CCTGTAATGT GAGTCAAGGA AGCTCTCTGC TGAGTGAGCT GGACCAGTAT 
CATGAGCAAG ATGATGATGG TCGGGAGAGG GACTCCATTC ATTCTAGTCA TAGCTATGGC 
AGCCTCTCCA AAGATGGCCA GGCTGGTTTG GGAGAACAAG AGAAAGCCTT GGAAGTGACA 
TGTGAATCAG AAAAAGAGAA AACAGGTGAA TCCAAGGAGA TGAGAGATGA TGCTACAATC 
CACCCTCCTT CAGATCTGGT GCTGCATAAG GACCATGTCC TAGGACCCCA GGAGAGTCTT 
CCTGAGGAGA CCGCATCATC GCCATTTACC CAGGCCAGAG CACACTGGTT CCGAGCGGTT 
ACCAAGGTCC GACTCCAACT GCAGGAGATT TCAGATGATG GTGACCCCTC CTTGCCTCAG 
TGGCTCCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGA 
AGGAAGAAGC CACTGCCACT TGTCAGTGAT CTGTCACTGG TCCAGTCACG GAAAGCTGGG 
ATTACATCTG CTATGGCTAC ACGTACCTCT CTCAAGGATG AAGAACTGAA ATCTCATGTG 
TATAAGAAAA CTCTGCAGGC CTTAATCTAC CCCATCTCGT GTACCACGCC CCACAACTTT 
GAGGTCTGGT CTGCCACTAC ACCTACCTAT TGCTATGAGT GTGAAGGCTT GCTCTGGGGC 
CTTGCCCGGC AAGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG 
GACCTGCTCA ATGCTGACTG CCTACAGCGG GCTGCTGAAA AGAGCTCTAA GCATGGAGCT 
GAGGACCGAA CTCAGAACAT TATCATGGCC ATGAAGGACC GTATGAAGAT CCGAGAACGG 
AATAAACCAG AGATCTTTGA GGTTATCCGG GATGTGTTCA CAGTGAGCAA AGTTGCGCAT 
GTGCAGCAGA TGAAAACAGT AAAGCAGAGT GTCCTGGATG GCACCTCCAA GTGGTCAGCC 
AAAATTACCA TCACGGTGGT TTGTGCCCAG GGCCTACAAG CCAAAGACAA GACAGGATCC 
AGTGACCCTT ATGTGACTGT GCAAGTTGGG AAAACCAAGA AGCGCACCAA GACCATTTTT 
GGAAATTTGA ATCCTGTTTG GGAAGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAT 
CGAATTAAGG TACGCGTGTG GGATGAGGAT GATGACATCA AGTCCAGAGT AAAGCAGCGG 
CTAAAGCGAG AGTCAGATGA TTTCCTTGGC CAAACTATCA TTGAAGTTCG AACGCTGAGT 
GGAGAGATGG ATGTCTGGTA CAATCTGGAG AAGAGAACAG ACAAGTCAGC TGTCTCAGGG 
GCTATCCGAC TTCAAATCAG TGTGGAGATC AAGGGGGAGG AGAAGGTAGC CCCATACCAT 
GTACAATATA CATGTCTCCA TGAGAACCTT TTCCATTACC TCACAGACAT TCAGGGCAGT 
GGAGGAGTCT GGATCCCAGA GGCTCGGGGG GACGATGCAT GGAAGGTGTA CTTTGATGAG 
ACAGCCCAAG AAATTGTGGA TGAATTCGCT ATGCGCTATG GCATCGAGTC CATTTATCAG 
GCCATGACGC ACTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT ACCAGCCGTG 
ATGAGCACCT TACTGGCCAA CATCAATGCC TATTATGCTC ACACAACTGC CTCCACTAAC 
GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGTA AAGAAAGATT TGTAAAACTG 
CTGGACCAGC TACACAACTC ACTGAGGATA GACCTCTCTA CATACAGGAA TAACTTTCCT 
GCTGGGAGCC CTGAGCGGCT TCAGGATTTA AAGTCTACAG TGGATTTGCT GACCAGCATT 
ACTTTCTTCA GGATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTGGTA 
AAGGACTGTG TGAAAGCCTG CTTGAACTCT ACATATGAAT ACATCTTCAA CAACTGCCAT 
GACCTCTACA GTCACCAGTA TCAGCTGCAG GAACAACCTC TAGAGGAACC AGGGCCCAGC 
ATTCGGAACT TGGATTTCTG GCCCAAACTT ATCACACTCA TTGTTTCGAT CATAGAGGAG 
GATAAGAATT CCTACACACC TGTTCTGAGC CAGTTTCCTC AGGAGTTGAA CGTAGGAAAA 
GTCAGTGCAG AAGTGATGTG GCATCTGTTT GCTCAAGACA TGAAATATGC ACTGGAGGAA 
CACGAGAAAG ACCGGCTGTG TAAGAGTGCT GACTACATGA ACCTGCACTT CAAGGTGAAG 
TGGCTCCACA ATGAATATGT GCGCGATCTG CCTGCCCTCC AGGGGCAAGT GCCTGAGTAC 
CCAGCGTGGT TTGAGCAGTT TGTGCTACAG TGGCTAGATG AGAATGAAGA TGTGTCACTG 
GAATTCCTTC GTGGGGCCTT GGAACGAGAT AAGAAGGATG GGTTCCAGCA GACATCAGAG 
CATGCCTTGT TTTCCTGTTC TGTGGTGGAT GTCTTTACAC AACTCAACCA GAGCTTTGAG 
ATCATCCGGA AGTTGGAATG TCCAGACCCC AACATCCTTG CCCACTACAT GAGAAGGTTT 
GCTAAGACCA TTGGGAAAGT GCTGATGCAG TATGCAGACA TCTTATCAAA GAACTTCCCA 
GCTTACTGCA CAAAGGAGAG ACTGCCCTGT ATCCTGATGA ACAACATGCA GCAACTGAGG 
GTCCAGCTGG AGAAAATGTT TGAGGCCATG GGAGGCAAAG AGCTGGACTC CGAAGCTGCA 
GACAGTCTGA AGGAGCTGCA GGTGAAACTG AATACAGTTC TGGATGAGCT TAGCATGGTG 
TTTGGAAACA GCTTCCAGGT GCGGATTGAT GAATGTGTTC GACAAATGGC TGACATCCTG 
GGCCAAGTTC GGGGCACAGG GAATGCATCC CCTAACGCCA GGGCCTCGGT GGCTCAGGAT 
GCAGATAGTG TGCTGCGACC TCTCATGGAC TTCCTGGATG GCAACCTCAC GCTGTTTGCC 
ACTGTGTGTG AGAAGACAGT TCTGAAGCGG GTCCTGAAGG AGCTCTGGCG GGTGGTGATG 
AACACCATGG AGCGGGTTAT TGTCCTGCCC CCACTCACTG ACCAGACGGG CACCCAGCTG 
ATCTTAACTG CTGCCAAGGA GCTGAGCCAG CTTTCCAAAC TCAAGGACCA CATGGTACGG 
GAGGAAACAC GGAATCTCAC TCCAAAGCAG TGTGCTGTCC TCGACTTGGC CTTGGACACC 
ATCAAGCAAT ACTTCCATGC AGGAGGCAAT GGTCTGAAGA AAACCTTCCT GGAAAAGAGC 
CCAGACCTGC AGTCTCTGCG TTATGCCCTC TCTCTGTATA CACAGACTAC AGACACTCTC 
ATCAAGACAT TTGTGCGCTC ACAGACTGCC CAGGGGGCTG GTGTGGATGA CCCCGTGGGA 
GAAGTCTCTA TTCAGGTGGA CTTATTTACA CATCCTGGCA CTGGGGAGCA TAAGGTCACA 
GTGAAAGTGG TAGCAGCCAA TGACCTCAAG TGGCAGACAG CGGGTATGTT CCGGCCTTTT 
GTGGAAGTGA CCATGGTTGG CCCACACCAA AGTGATAAGA AGAGGAAGTT CACAACCAAG 
TCTAAAAGTA ACAACTGGAC TCCCAAGTAC AACGAGACGT TTCACTTCCT CCTGGGAAAT 
GAAGAGGGTC CAGAGGCCTA TGAATTGCAG ATCTGTGTGA AGGATTACTG CTTTGCCCGG 
GAGGATCGTG TGATAGGACT GGCAGTGATG CCCCTGAGGG ATGTGGCAGC TAAGGGCAGC 
TGTGCCTGCT GGTGCCCACT GGGCCGGAAG ATCCATATGG ATGAGACAGG CATGACCATT 
CTCCGGATTC TGTCTCAGAG GAGCAATGAC GAGGTAGCCC GAGAGTTCGT GAAGCTCAAA 
TCAGAGTCTC GATCCACAGA GGAAGGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_017320115.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175604.1 Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X2, mRNA.

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CloneID	OMu32852
Clone ID Related Accession (Same CDS sequence)	XM_017320115.1 , XM_017320115.2 , XM_006537786.3 , XM_006537786.4
Accession Version	XM_017320115.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGCAGGAACA ACCTCTAGAG GAACCAGGGC CCAGCATTCG GAACTTGGAT 
TTCTGGCCCA AACTTATCAC ACTCATTGTT TCGATCATAG AGGAGGATAA GAATTCCTAC 
ACACCTGTTC TGAGCCAGTT TCCTCAGGAG TTGAACGTAG GAAAAGTCAG TGCAGAAGTG 
ATGTGGCATC TGTTTGCTCA AGACATGAAA TATGCACTGG AGGAACACGA GAAAGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TATGTGCGCG ATCTGCCTGC CCTCCAGGGG CAAGTGCCTG AGTACCCAGC GTGGTTTGAG 
CAGTTTGTGC TACAGTGGCT AGATGAGAAT GAAGATGTGT CACTGGAATT CCTTCGTGGG 
GCCTTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC CTTGTTTTCC 
TGTTCTGTGG TGGATGTCTT TACACAACTC AACCAGAGCT TTGAGATCAT CCGGAAGTTG 
GAATGTCCAG ACCCCAACAT CCTTGCCCAC TACATGAGAA GGTTTGCTAA GACCATTGGG 
AAAGTGCTGA TGCAGTATGC AGACATCTTA TCAAAGAACT TCCCAGCTTA CTGCACAAAG 
GAGAGACTGC CCTGTATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAAGAGCTG GACTCCGAAG CTGCAGACAG TCTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTTAGCA TGGTGTTTGG AAACAGCTTC 
CAGGTGCGGA TTGATGAATG TGTTCGACAA ATGGCTGACA TCCTGGGCCA AGTTCGGGGC 
ACAGGGAATG CATCCCCTAA CGCCAGGGCC TCGGTGGCTC AGGATGCAGA TAGTGTGCTG 
CGACCTCTCA TGGACTTCCT GGATGGCAAC CTCACGCTGT TTGCCACTGT GTGTGAGAAG 
ACAGTTCTGA AGCGGGTCCT GAAGGAGCTC TGGCGGGTGG TGATGAACAC CATGGAGCGG 
GTTATTGTCC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT AACTGCTGCC 
AAGGAGCTGA GCCAGCTTTC CAAACTCAAG GACCACATGG TACGGGAGGA AACACGGAAT 
CTCACTCCAA AGCAGTGTGC TGTCCTCGAC TTGGCCTTGG ACACCATCAA GCAATACTTC 
CATGCAGGAG GCAATGGTCT GAAGAAAACC TTCCTGGAAA AGAGCCCAGA CCTGCAGTCT 
CTGCGTTATG CCCTCTCTCT GTATACACAG ACTACAGACA CTCTCATCAA GACATTTGTG 
CGCTCACAGA CTGCCCAGGT ACATGATGGG AAAGGTATTA GGTTTACTGC TAACGAGGAC 
ATTCGGCCAG AAAAGGGGGC TGGTGTGGAT GACCCCGTGG GAGAAGTCTC TATTCAGGTG 
GACTTATTTA CACATCCTGG CACTGGGGAG CATAAGGTCA CAGTGAAAGT GGTAGCAGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCTT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGATAA GAAGAGGAAG TTCACAACCA AGTCTAAAAG TAACAACTGG 
ACTCCCAAGT ACAACGAGAC GTTTCACTTC CTCCTGGGAA ATGAAGAGGG TCCAGAGGCC 
TATGAATTGC AGATCTGTGT GAAGGATTAC TGCTTTGCCC GGGAGGATCG TGTGATAGGA 
CTGGCAGTGA TGCCCCTGAG GGATGTGGCA GCTAAGGGCA GCTGTGCCTG CTGGTGCCCA 
CTGGGCCGGA AGATCCATAT GGATGAGACA GGCATGACCA TTCTCCGGAT TCTGTCTCAG 
AGGAGCAATG ACGAGGTAGC CCGAGAGTTC GTGAAGCTCA AATCAGAGTC TCGATCCACA 
GAGGAAGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175604.1
CDS	258..6212
Translation	MKRLLRESEEEIMLTLGPSSSLSPDKVRAETVCSVKSKSTGPTGSLPEDNFPLPCCDSTTSGERDGDLPQLCSFEQQANNQPSLASTACASGSDSRELSPASITNCSDPSERNQARPILPRGPGQRCRYEQQEPLGDVVEYIIRELQGISRLQSEIAELQQHLNQVQGSVDEVSSCVDSVLSEIEGLHVGSSSLGKVCSGEKAQELHVERSREEAILYLYGLPEHDGENTMELVDNFLAKHLCVNGMQCNRYIQEAYRTGTAPAPRPTVVKLVHLEHRDLILQKSILLQSVGVRVATREEPVWSEGCEDPPKESLSCLQQLQDHSWNSLNQDKPALRLETGNRKQMTEPHQIRTQNQQRGPQASEHQGPRLLPKDGLAKQSDVSKLQEEVKGTSGASWVTSDCCGELSLLHPLEGSSSVLISKEEDCEKLQIFKQGRQEHEACKVTKLQSDCSNSIKDSSCLSLSGPLKADMVNTEDKMLGCEAGLDILSPKQLEDLLADKSRRFATLSPDSTMEEVIIGPETLSNMVHIDLNEEENCTAQVLKDVFDKSSRVLADSQEDEDVEIKFHTTKLSRAIQHFRLALQGVFQKLENNGSISPEDLESNESGSQSENSDRLLWTVSSGGAHDCSVESPASQGSESLLSVVSGGVGISIQGDQTSQAPSNFSLASNNSPFANSLLSFPLAPCLGSETCSRPDSPNQGKLSLEQVCAETIYLNKCINNFKNVLREKRLRQKKLLQELVQTASHLSVEDIPSEGNQEAIQISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_017320115.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320115.1

ORF Insert Sequence:

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGCAGGAACA ACCTCTAGAG GAACCAGGGC CCAGCATTCG GAACTTGGAT 
TTCTGGCCCA AACTTATCAC ACTCATTGTT TCGATCATAG AGGAGGATAA GAATTCCTAC 
ACACCTGTTC TGAGCCAGTT TCCTCAGGAG TTGAACGTAG GAAAAGTCAG TGCAGAAGTG 
ATGTGGCATC TGTTTGCTCA AGACATGAAA TATGCACTGG AGGAACACGA GAAAGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TATGTGCGCG ATCTGCCTGC CCTCCAGGGG CAAGTGCCTG AGTACCCAGC GTGGTTTGAG 
CAGTTTGTGC TACAGTGGCT AGATGAGAAT GAAGATGTGT CACTGGAATT CCTTCGTGGG 
GCCTTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC CTTGTTTTCC 
TGTTCTGTGG TGGATGTCTT TACACAACTC AACCAGAGCT TTGAGATCAT CCGGAAGTTG 
GAATGTCCAG ACCCCAACAT CCTTGCCCAC TACATGAGAA GGTTTGCTAA GACCATTGGG 
AAAGTGCTGA TGCAGTATGC AGACATCTTA TCAAAGAACT TCCCAGCTTA CTGCACAAAG 
GAGAGACTGC CCTGTATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAAGAGCTG GACTCCGAAG CTGCAGACAG TCTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTTAGCA TGGTGTTTGG AAACAGCTTC 
CAGGTGCGGA TTGATGAATG TGTTCGACAA ATGGCTGACA TCCTGGGCCA AGTTCGGGGC 
ACAGGGAATG CATCCCCTAA CGCCAGGGCC TCGGTGGCTC AGGATGCAGA TAGTGTGCTG 
CGACCTCTCA TGGACTTCCT GGATGGCAAC CTCACGCTGT TTGCCACTGT GTGTGAGAAG 
ACAGTTCTGA AGCGGGTCCT GAAGGAGCTC TGGCGGGTGG TGATGAACAC CATGGAGCGG 
GTTATTGTCC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT AACTGCTGCC 
AAGGAGCTGA GCCAGCTTTC CAAACTCAAG GACCACATGG TACGGGAGGA AACACGGAAT 
CTCACTCCAA AGCAGTGTGC TGTCCTCGAC TTGGCCTTGG ACACCATCAA GCAATACTTC 
CATGCAGGAG GCAATGGTCT GAAGAAAACC TTCCTGGAAA AGAGCCCAGA CCTGCAGTCT 
CTGCGTTATG CCCTCTCTCT GTATACACAG ACTACAGACA CTCTCATCAA GACATTTGTG 
CGCTCACAGA CTGCCCAGGT ACATGATGGG AAAGGTATTA GGTTTACTGC TAACGAGGAC 
ATTCGGCCAG AAAAGGGGGC TGGTGTGGAT GACCCCGTGG GAGAAGTCTC TATTCAGGTG 
GACTTATTTA CACATCCTGG CACTGGGGAG CATAAGGTCA CAGTGAAAGT GGTAGCAGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCTT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGATAA GAAGAGGAAG TTCACAACCA AGTCTAAAAG TAACAACTGG 
ACTCCCAAGT ACAACGAGAC GTTTCACTTC CTCCTGGGAA ATGAAGAGGG TCCAGAGGCC 
TATGAATTGC AGATCTGTGT GAAGGATTAC TGCTTTGCCC GGGAGGATCG TGTGATAGGA 
CTGGCAGTGA TGCCCCTGAG GGATGTGGCA GCTAAGGGCA GCTGTGCCTG CTGGTGCCCA 
CTGGGCCGGA AGATCCATAT GGATGAGACA GGCATGACCA TTCTCCGGAT TCTGTCTCAG 
AGGAGCAATG ACGAGGTAGC CCGAGAGTTC GTGAAGCTCA AATCAGAGTC TCGATCCACA 
GAGGAAGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537786.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537849.1 Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X1, mRNA.

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CloneID	OMu32852
Clone ID Related Accession (Same CDS sequence)	XM_017320115.1 , XM_017320115.2 , XM_006537786.3 , XM_006537786.4
Accession Version	XM_006537786.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537786.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGCAGGAACA ACCTCTAGAG GAACCAGGGC CCAGCATTCG GAACTTGGAT 
TTCTGGCCCA AACTTATCAC ACTCATTGTT TCGATCATAG AGGAGGATAA GAATTCCTAC 
ACACCTGTTC TGAGCCAGTT TCCTCAGGAG TTGAACGTAG GAAAAGTCAG TGCAGAAGTG 
ATGTGGCATC TGTTTGCTCA AGACATGAAA TATGCACTGG AGGAACACGA GAAAGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TATGTGCGCG ATCTGCCTGC CCTCCAGGGG CAAGTGCCTG AGTACCCAGC GTGGTTTGAG 
CAGTTTGTGC TACAGTGGCT AGATGAGAAT GAAGATGTGT CACTGGAATT CCTTCGTGGG 
GCCTTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC CTTGTTTTCC 
TGTTCTGTGG TGGATGTCTT TACACAACTC AACCAGAGCT TTGAGATCAT CCGGAAGTTG 
GAATGTCCAG ACCCCAACAT CCTTGCCCAC TACATGAGAA GGTTTGCTAA GACCATTGGG 
AAAGTGCTGA TGCAGTATGC AGACATCTTA TCAAAGAACT TCCCAGCTTA CTGCACAAAG 
GAGAGACTGC CCTGTATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAAGAGCTG GACTCCGAAG CTGCAGACAG TCTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTTAGCA TGGTGTTTGG AAACAGCTTC 
CAGGTGCGGA TTGATGAATG TGTTCGACAA ATGGCTGACA TCCTGGGCCA AGTTCGGGGC 
ACAGGGAATG CATCCCCTAA CGCCAGGGCC TCGGTGGCTC AGGATGCAGA TAGTGTGCTG 
CGACCTCTCA TGGACTTCCT GGATGGCAAC CTCACGCTGT TTGCCACTGT GTGTGAGAAG 
ACAGTTCTGA AGCGGGTCCT GAAGGAGCTC TGGCGGGTGG TGATGAACAC CATGGAGCGG 
GTTATTGTCC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT AACTGCTGCC 
AAGGAGCTGA GCCAGCTTTC CAAACTCAAG GACCACATGG TACGGGAGGA AACACGGAAT 
CTCACTCCAA AGCAGTGTGC TGTCCTCGAC TTGGCCTTGG ACACCATCAA GCAATACTTC 
CATGCAGGAG GCAATGGTCT GAAGAAAACC TTCCTGGAAA AGAGCCCAGA CCTGCAGTCT 
CTGCGTTATG CCCTCTCTCT GTATACACAG ACTACAGACA CTCTCATCAA GACATTTGTG 
CGCTCACAGA CTGCCCAGGT ACATGATGGG AAAGGTATTA GGTTTACTGC TAACGAGGAC 
ATTCGGCCAG AAAAGGGGGC TGGTGTGGAT GACCCCGTGG GAGAAGTCTC TATTCAGGTG 
GACTTATTTA CACATCCTGG CACTGGGGAG CATAAGGTCA CAGTGAAAGT GGTAGCAGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCTT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGATAA GAAGAGGAAG TTCACAACCA AGTCTAAAAG TAACAACTGG 
ACTCCCAAGT ACAACGAGAC GTTTCACTTC CTCCTGGGAA ATGAAGAGGG TCCAGAGGCC 
TATGAATTGC AGATCTGTGT GAAGGATTAC TGCTTTGCCC GGGAGGATCG TGTGATAGGA 
CTGGCAGTGA TGCCCCTGAG GGATGTGGCA GCTAAGGGCA GCTGTGCCTG CTGGTGCCCA 
CTGGGCCGGA AGATCCATAT GGATGAGACA GGCATGACCA TTCTCCGGAT TCTGTCTCAG 
AGGAGCAATG ACGAGGTAGC CCGAGAGTTC GTGAAGCTCA AATCAGAGTC TCGATCCACA 
GAGGAAGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537849.1
CDS	761..6715
Translation	MKRLLRESEEEIMLTLGPSSSLSPDKVRAETVCSVKSKSTGPTGSLPEDNFPLPCCDSTTSGERDGDLPQLCSFEQQANNQPSLASTACASGSDSRELSPASITNCSDPSERNQARPILPRGPGQRCRYEQQEPLGDVVEYIIRELQGISRLQSEIAELQQHLNQVQGSVDEVSSCVDSVLSEIEGLHVGSSSLGKVCSGEKAQELHVERSREEAILYLYGLPEHDGENTMELVDNFLAKHLCVNGMQCNRYIQEAYRTGTAPAPRPTVVKLVHLEHRDLILQKSILLQSVGVRVATREEPVWSEGCEDPPKESLSCLQQLQDHSWNSLNQDKPALRLETGNRKQMTEPHQIRTQNQQRGPQASEHQGPRLLPKDGLAKQSDVSKLQEEVKGTSGASWVTSDCCGELSLLHPLEGSSSVLISKEEDCEKLQIFKQGRQEHEACKVTKLQSDCSNSIKDSSCLSLSGPLKADMVNTEDKMLGCEAGLDILSPKQLEDLLADKSRRFATLSPDSTMEEVIIGPETLSNMVHIDLNEEENCTAQVLKDVFDKSSRVLADSQEDEDVEIKFHTTKLSRAIQHFRLALQGVFQKLENNGSISPEDLESNESGSQSENSDRLLWTVSSGGAHDCSVESPASQGSESLLSVVSGGVGISIQGDQTSQAPSNFSLASNNSPFANSLLSFPLAPCLGSETCSRPDSPNQGKLSLEQVCAETIYLNKCINNFKNVLREKRLRQKKLLQELVQTASHLSVEDIPSEGNQEAIQISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537786.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537786.3

ORF Insert Sequence:

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGCAGGAACA ACCTCTAGAG GAACCAGGGC CCAGCATTCG GAACTTGGAT 
TTCTGGCCCA AACTTATCAC ACTCATTGTT TCGATCATAG AGGAGGATAA GAATTCCTAC 
ACACCTGTTC TGAGCCAGTT TCCTCAGGAG TTGAACGTAG GAAAAGTCAG TGCAGAAGTG 
ATGTGGCATC TGTTTGCTCA AGACATGAAA TATGCACTGG AGGAACACGA GAAAGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TATGTGCGCG ATCTGCCTGC CCTCCAGGGG CAAGTGCCTG AGTACCCAGC GTGGTTTGAG 
CAGTTTGTGC TACAGTGGCT AGATGAGAAT GAAGATGTGT CACTGGAATT CCTTCGTGGG 
GCCTTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC CTTGTTTTCC 
TGTTCTGTGG TGGATGTCTT TACACAACTC AACCAGAGCT TTGAGATCAT CCGGAAGTTG 
GAATGTCCAG ACCCCAACAT CCTTGCCCAC TACATGAGAA GGTTTGCTAA GACCATTGGG 
AAAGTGCTGA TGCAGTATGC AGACATCTTA TCAAAGAACT TCCCAGCTTA CTGCACAAAG 
GAGAGACTGC CCTGTATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAAGAGCTG GACTCCGAAG CTGCAGACAG TCTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTTAGCA TGGTGTTTGG AAACAGCTTC 
CAGGTGCGGA TTGATGAATG TGTTCGACAA ATGGCTGACA TCCTGGGCCA AGTTCGGGGC 
ACAGGGAATG CATCCCCTAA CGCCAGGGCC TCGGTGGCTC AGGATGCAGA TAGTGTGCTG 
CGACCTCTCA TGGACTTCCT GGATGGCAAC CTCACGCTGT TTGCCACTGT GTGTGAGAAG 
ACAGTTCTGA AGCGGGTCCT GAAGGAGCTC TGGCGGGTGG TGATGAACAC CATGGAGCGG 
GTTATTGTCC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT AACTGCTGCC 
AAGGAGCTGA GCCAGCTTTC CAAACTCAAG GACCACATGG TACGGGAGGA AACACGGAAT 
CTCACTCCAA AGCAGTGTGC TGTCCTCGAC TTGGCCTTGG ACACCATCAA GCAATACTTC 
CATGCAGGAG GCAATGGTCT GAAGAAAACC TTCCTGGAAA AGAGCCCAGA CCTGCAGTCT 
CTGCGTTATG CCCTCTCTCT GTATACACAG ACTACAGACA CTCTCATCAA GACATTTGTG 
CGCTCACAGA CTGCCCAGGT ACATGATGGG AAAGGTATTA GGTTTACTGC TAACGAGGAC 
ATTCGGCCAG AAAAGGGGGC TGGTGTGGAT GACCCCGTGG GAGAAGTCTC TATTCAGGTG 
GACTTATTTA CACATCCTGG CACTGGGGAG CATAAGGTCA CAGTGAAAGT GGTAGCAGCC 
AATGACCTCA AGTGGCAGAC AGCGGGTATG TTCCGGCCTT TTGTGGAAGT GACCATGGTT 
GGCCCACACC AAAGTGATAA GAAGAGGAAG TTCACAACCA AGTCTAAAAG TAACAACTGG 
ACTCCCAAGT ACAACGAGAC GTTTCACTTC CTCCTGGGAA ATGAAGAGGG TCCAGAGGCC 
TATGAATTGC AGATCTGTGT GAAGGATTAC TGCTTTGCCC GGGAGGATCG TGTGATAGGA 
CTGGCAGTGA TGCCCCTGAG GGATGTGGCA GCTAAGGGCA GCTGTGCCTG CTGGTGCCCA 
CTGGGCCGGA AGATCCATAT GGATGAGACA GGCATGACCA TTCTCCGGAT TCTGTCTCAG 
AGGAGCAATG ACGAGGTAGC CCGAGAGTTC GTGAAGCTCA AATCAGAGTC TCGATCCACA 
GAGGAAGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537788.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537851.1 Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X3, mRNA.

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CloneID	OMu32853
Clone ID Related Accession (Same CDS sequence)	XM_006537788.3 , XM_006537788.4
Accession Version	XM_006537788.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537788.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGGAACAACC TCTAGAGGAA CCAGGGCCCA GCATTCGGAA CTTGGATTTC 
TGGCCCAAAC TTATCACACT CATTGTTTCG ATCATAGAGG AGGATAAGAA TTCCTACACA 
CCTGTTCTGA GCCAGTTTCC TCAGGAGTTG AACGTAGGAA AAGTCAGTGC AGAAGTGATG 
TGGCATCTGT TTGCTCAAGA CATGAAATAT GCACTGGAGG AACACGAGAA AGACCGGCTG 
TGTAAGAGTG CTGACTACAT GAACCTGCAC TTCAAGGTGA AGTGGCTCCA CAATGAATAT 
GTGCGCGATC TGCCTGCCCT CCAGGGGCAA GTGCCTGAGT ACCCAGCGTG GTTTGAGCAG 
TTTGTGCTAC AGTGGCTAGA TGAGAATGAA GATGTGTCAC TGGAATTCCT TCGTGGGGCC 
TTGGAACGAG ATAAGAAGGA TGGGTTCCAG CAGACATCAG AGCATGCCTT GTTTTCCTGT 
TCTGTGGTGG ATGTCTTTAC ACAACTCAAC CAGAGCTTTG AGATCATCCG GAAGTTGGAA 
TGTCCAGACC CCAACATCCT TGCCCACTAC ATGAGAAGGT TTGCTAAGAC CATTGGGAAA 
GTGCTGATGC AGTATGCAGA CATCTTATCA AAGAACTTCC CAGCTTACTG CACAAAGGAG 
AGACTGCCCT GTATCCTGAT GAACAACATG CAGCAACTGA GGGTCCAGCT GGAGAAAATG 
TTTGAGGCCA TGGGAGGCAA AGAGCTGGAC TCCGAAGCTG CAGACAGTCT GAAGGAGCTG 
CAGGTGAAAC TGAATACAGT TCTGGATGAG CTTAGCATGG TGTTTGGAAA CAGCTTCCAG 
GTGCGGATTG ATGAATGTGT TCGACAAATG GCTGACATCC TGGGCCAAGT TCGGGGCACA 
GGGAATGCAT CCCCTAACGC CAGGGCCTCG GTGGCTCAGG ATGCAGATAG TGTGCTGCGA 
CCTCTCATGG ACTTCCTGGA TGGCAACCTC ACGCTGTTTG CCACTGTGTG TGAGAAGACA 
GTTCTGAAGC GGGTCCTGAA GGAGCTCTGG CGGGTGGTGA TGAACACCAT GGAGCGGGTT 
ATTGTCCTGC CCCCACTCAC TGACCAGACG GGCACCCAGC TGATCTTAAC TGCTGCCAAG 
GAGCTGAGCC AGCTTTCCAA ACTCAAGGAC CACATGGTAC GGGAGGAAAC ACGGAATCTC 
ACTCCAAAGC AGTGTGCTGT CCTCGACTTG GCCTTGGACA CCATCAAGCA ATACTTCCAT 
GCAGGAGGCA ATGGTCTGAA GAAAACCTTC CTGGAAAAGA GCCCAGACCT GCAGTCTCTG 
CGTTATGCCC TCTCTCTGTA TACACAGACT ACAGACACTC TCATCAAGAC ATTTGTGCGC 
TCACAGACTG CCCAGGTACA TGATGGGAAA GGTATTAGGT TTACTGCTAA CGAGGACATT 
CGGCCAGAAA AGGGGGCTGG TGTGGATGAC CCCGTGGGAG AAGTCTCTAT TCAGGTGGAC 
TTATTTACAC ATCCTGGCAC TGGGGAGCAT AAGGTCACAG TGAAAGTGGT AGCAGCCAAT 
GACCTCAAGT GGCAGACAGC GGGTATGTTC CGGCCTTTTG TGGAAGTGAC CATGGTTGGC 
CCACACCAAA GTGATAAGAA GAGGAAGTTC ACAACCAAGT CTAAAAGTAA CAACTGGACT 
CCCAAGTACA ACGAGACGTT TCACTTCCTC CTGGGAAATG AAGAGGGTCC AGAGGCCTAT 
GAATTGCAGA TCTGTGTGAA GGATTACTGC TTTGCCCGGG AGGATCGTGT GATAGGACTG 
GCAGTGATGC CCCTGAGGGA TGTGGCAGCT AAGGGCAGCT GTGCCTGCTG GTGCCCACTG 
GGCCGGAAGA TCCATATGGA TGAGACAGGC ATGACCATTC TCCGGATTCT GTCTCAGAGG 
AGCAATGACG AGGTAGCCCG AGAGTTCGTG AAGCTCAAAT CAGAGTCTCG ATCCACAGAG 
GAAGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537851.1
CDS	938..6889
Translation	MKRLLRESEEEIMLTLGPSSSLSPDKVRAETVCSVKSKSTGPTGSLPEDNFPLPCCDSTTSGERDGDLPQLCSFEQQANNQPSLASTACASGSDSRELSPASITNCSDPSERNQARPILPRGPGQRCRYEQQEPLGDVVEYIIRELQGISRLQSEIAELQQHLNQVQGSVDEVSSCVDSVLSEIEGLHVGSSSLGKVCSGEKAQELHVERSREEAILYLYGLPEHDGENTMELVDNFLAKHLCVNGMQCNRYIQEAYRTGTAPAPRPTVVKLVHLEHRDLILQKSILLQSVGVRVATREEPVWSEGCEDPPKESLSCLQQLQDHSWNSLNQDKPALRLETGNRKQMTEPHQIRTQNQQRGPQASEHQGPRLLPKDGLAKQSDVSKLQEEVKGTSGASWVTSDCCGELSLLHPLEGSSSVLISKEEDCEKLQIFKQGRQEHEACKVTKLQSDCSNSIKDSSCLSLSGPLKADMVNTEDKMLGCEAGLDILSPKQLEDLLADKSRRFATLSPDSTMEEVIIGPETLSNMVHIDLNEEENCTAQVLKDVFDKSSRVLADSQEDEDVEIKFHTTKLSRAIQHFRLALQGVFQKLENNGSISPEDLESNESGSQSENSDRLLWTVSSGGAHDCSVESPASQGSESLLSVVSGGVGISIQGDQTSQAPSNFSLASNNSPFANSLLSFPLAPCLGSETCSRPDSPNQGKLSLEQVCAETIYLNKCINNFKNVLREKRLRQKKLLQELVQTASHLSVEDIPSEGNQEAIQISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537788.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537788.3

ORF Insert Sequence:

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGGAACAACC TCTAGAGGAA CCAGGGCCCA GCATTCGGAA CTTGGATTTC 
TGGCCCAAAC TTATCACACT CATTGTTTCG ATCATAGAGG AGGATAAGAA TTCCTACACA 
CCTGTTCTGA GCCAGTTTCC TCAGGAGTTG AACGTAGGAA AAGTCAGTGC AGAAGTGATG 
TGGCATCTGT TTGCTCAAGA CATGAAATAT GCACTGGAGG AACACGAGAA AGACCGGCTG 
TGTAAGAGTG CTGACTACAT GAACCTGCAC TTCAAGGTGA AGTGGCTCCA CAATGAATAT 
GTGCGCGATC TGCCTGCCCT CCAGGGGCAA GTGCCTGAGT ACCCAGCGTG GTTTGAGCAG 
TTTGTGCTAC AGTGGCTAGA TGAGAATGAA GATGTGTCAC TGGAATTCCT TCGTGGGGCC 
TTGGAACGAG ATAAGAAGGA TGGGTTCCAG CAGACATCAG AGCATGCCTT GTTTTCCTGT 
TCTGTGGTGG ATGTCTTTAC ACAACTCAAC CAGAGCTTTG AGATCATCCG GAAGTTGGAA 
TGTCCAGACC CCAACATCCT TGCCCACTAC ATGAGAAGGT TTGCTAAGAC CATTGGGAAA 
GTGCTGATGC AGTATGCAGA CATCTTATCA AAGAACTTCC CAGCTTACTG CACAAAGGAG 
AGACTGCCCT GTATCCTGAT GAACAACATG CAGCAACTGA GGGTCCAGCT GGAGAAAATG 
TTTGAGGCCA TGGGAGGCAA AGAGCTGGAC TCCGAAGCTG CAGACAGTCT GAAGGAGCTG 
CAGGTGAAAC TGAATACAGT TCTGGATGAG CTTAGCATGG TGTTTGGAAA CAGCTTCCAG 
GTGCGGATTG ATGAATGTGT TCGACAAATG GCTGACATCC TGGGCCAAGT TCGGGGCACA 
GGGAATGCAT CCCCTAACGC CAGGGCCTCG GTGGCTCAGG ATGCAGATAG TGTGCTGCGA 
CCTCTCATGG ACTTCCTGGA TGGCAACCTC ACGCTGTTTG CCACTGTGTG TGAGAAGACA 
GTTCTGAAGC GGGTCCTGAA GGAGCTCTGG CGGGTGGTGA TGAACACCAT GGAGCGGGTT 
ATTGTCCTGC CCCCACTCAC TGACCAGACG GGCACCCAGC TGATCTTAAC TGCTGCCAAG 
GAGCTGAGCC AGCTTTCCAA ACTCAAGGAC CACATGGTAC GGGAGGAAAC ACGGAATCTC 
ACTCCAAAGC AGTGTGCTGT CCTCGACTTG GCCTTGGACA CCATCAAGCA ATACTTCCAT 
GCAGGAGGCA ATGGTCTGAA GAAAACCTTC CTGGAAAAGA GCCCAGACCT GCAGTCTCTG 
CGTTATGCCC TCTCTCTGTA TACACAGACT ACAGACACTC TCATCAAGAC ATTTGTGCGC 
TCACAGACTG CCCAGGTACA TGATGGGAAA GGTATTAGGT TTACTGCTAA CGAGGACATT 
CGGCCAGAAA AGGGGGCTGG TGTGGATGAC CCCGTGGGAG AAGTCTCTAT TCAGGTGGAC 
TTATTTACAC ATCCTGGCAC TGGGGAGCAT AAGGTCACAG TGAAAGTGGT AGCAGCCAAT 
GACCTCAAGT GGCAGACAGC GGGTATGTTC CGGCCTTTTG TGGAAGTGAC CATGGTTGGC 
CCACACCAAA GTGATAAGAA GAGGAAGTTC ACAACCAAGT CTAAAAGTAA CAACTGGACT 
CCCAAGTACA ACGAGACGTT TCACTTCCTC CTGGGAAATG AAGAGGGTCC AGAGGCCTAT 
GAATTGCAGA TCTGTGTGAA GGATTACTGC TTTGCCCGGG AGGATCGTGT GATAGGACTG 
GCAGTGATGC CCCTGAGGGA TGTGGCAGCT AAGGGCAGCT GTGCCTGCTG GTGCCCACTG 
GGCCGGAAGA TCCATATGGA TGAGACAGGC ATGACCATTC TCCGGATTCT GTCTCAGAGG 
AGCAATGACG AGGTAGCCCG AGAGTTCGTG AAGCTCAAAT CAGAGTCTCG ATCCACAGAG 
GAAGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537789.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537852.1 Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X4, mRNA.

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CloneID	OMu32854
Clone ID Related Accession (Same CDS sequence)	XM_006537789.3 , XM_006537789.4
Accession Version	XM_006537789.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537789.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGTCACTGGT CCAGTCACGG AAAGCTGGGA TTACATCTGC TATGGCTACA 
CGTACCTCTC TCAAGGATGA AGAACTGAAA TCTCATGTGT ATAAGAAAAC TCTGCAGGCC 
TTAATCTACC CCATCTCGTG TACCACGCCC CACAACTTTG AGGTCTGGTC TGCCACTACA 
CCTACCTATT GCTATGAGTG TGAAGGCTTG CTCTGGGGCC TTGCCCGGCA AGGCATGCGC 
TGCAGCGAGT GTGGAGTCAA GTGCCATGAG AAGTGCCAGG ACCTGCTCAA TGCTGACTGC 
CTACAGCGGG CTGCTGAAAA GAGCTCTAAG CATGGAGCTG AGGACCGAAC TCAGAACATT 
ATCATGGCCA TGAAGGACCG TATGAAGATC CGAGAACGGA ATAAACCAGA GATCTTTGAG 
GTTATCCGGG ATGTGTTCAC AGTGAGCAAA GTTGCGCATG TGCAGCAGAT GAAAACAGTA 
AAGCAGAGTG TCCTGGATGG CACCTCCAAG TGGTCAGCCA AAATTACCAT CACGGTGGTT 
TGTGCCCAGG GCCTACAAGC CAAAGACAAG ACAGGATCCA GTGACCCTTA TGTGACTGTG 
CAAGTTGGGA AAACCAAGAA GCGCACCAAG ACCATTTTTG GAAATTTGAA TCCTGTTTGG 
GAAGAGAAGT TCCATTTTGA GTGCCACAAC TCTTCTGATC GAATTAAGGT ACGCGTGTGG 
GATGAGGATG ATGACATCAA GTCCAGAGTA AAGCAGCGGC TAAAGCGAGA GTCAGATGAT 
TTCCTTGGCC AAACTATCAT TGAAGTTCGA ACGCTGAGTG GAGAGATGGA TGTCTGGTAC 
AATCTGGAGA AGAGAACAGA CAAGTCAGCT GTCTCAGGGG CTATCCGACT TCAAATCAGT 
GTGGAGATCA AGGGGGAGGA GAAGGTAGCC CCATACCATG TACAATATAC ATGTCTCCAT 
GAGAACCTTT TCCATTACCT CACAGACATT CAGGGCAGTG GAGGAGTCTG GATCCCAGAG 
GCTCGGGGGG ACGATGCATG GAAGGTGTAC TTTGATGAGA CAGCCCAAGA AATTGTGGAT 
GAATTCGCTA TGCGCTATGG CATCGAGTCC ATTTATCAGG CCATGACGCA CTTTGCATGT 
TTGTCATCCA AGTACATGTG TCCTGGTGTA CCAGCCGTGA TGAGCACCTT ACTGGCCAAC 
ATCAATGCCT ATTATGCTCA CACAACTGCC TCCACTAACG TCTCTGCATC TGATCGTTTT 
GCAGCCTCCA ACTTTGGTAA AGAAAGATTT GTAAAACTGC TGGACCAGCT ACACAACTCA 
CTGAGGATAG ACCTCTCTAC ATACAGGAAT AACTTTCCTG CTGGGAGCCC TGAGCGGCTT 
CAGGATTTAA AGTCTACAGT GGATTTGCTG ACCAGCATTA CTTTCTTCAG GATGAAGGTG 
CAAGAACTTC AGAGCCCTCC AAGAGCCAGC CAGGTGGTAA AGGACTGTGT GAAAGCCTGC 
TTGAACTCTA CATATGAATA CATCTTCAAC AACTGCCATG ACCTCTACAG TCACCAGTAT 
CAGCTGCAGG AACAACCTCT AGAGGAACCA GGGCCCAGCA TTCGGAACTT GGATTTCTGG 
CCCAAACTTA TCACACTCAT TGTTTCGATC ATAGAGGAGG ATAAGAATTC CTACACACCT 
GTTCTGAGCC AGTTTCCTCA GGAGTTGAAC GTAGGAAAAG TCAGTGCAGA AGTGATGTGG 
CATCTGTTTG CTCAAGACAT GAAATATGCA CTGGAGGAAC ACGAGAAAGA CCGGCTGTGT 
AAGAGTGCTG ACTACATGAA CCTGCACTTC AAGGTGAAGT GGCTCCACAA TGAATATGTG 
CGCGATCTGC CTGCCCTCCA GGGGCAAGTG CCTGAGTACC CAGCGTGGTT TGAGCAGTTT 
GTGCTACAGT GGCTAGATGA GAATGAAGAT GTGTCACTGG AATTCCTTCG TGGGGCCTTG 
GAACGAGATA AGAAGGATGG GTTCCAGCAG ACATCAGAGC ATGCCTTGTT TTCCTGTTCT 
GTGGTGGATG TCTTTACACA ACTCAACCAG AGCTTTGAGA TCATCCGGAA GTTGGAATGT 
CCAGACCCCA ACATCCTTGC CCACTACATG AGAAGGTTTG CTAAGACCAT TGGGAAAGTG 
CTGATGCAGT ATGCAGACAT CTTATCAAAG AACTTCCCAG CTTACTGCAC AAAGGAGAGA 
CTGCCCTGTA TCCTGATGAA CAACATGCAG CAACTGAGGG TCCAGCTGGA GAAAATGTTT 
GAGGCCATGG GAGGCAAAGA GCTGGACTCC GAAGCTGCAG ACAGTCTGAA GGAGCTGCAG 
GTGAAACTGA ATACAGTTCT GGATGAGCTT AGCATGGTGT TTGGAAACAG CTTCCAGGTG 
CGGATTGATG AATGTGTTCG ACAAATGGCT GACATCCTGG GCCAAGTTCG GGGCACAGGG 
AATGCATCCC CTAACGCCAG GGCCTCGGTG GCTCAGGATG CAGATAGTGT GCTGCGACCT 
CTCATGGACT TCCTGGATGG CAACCTCACG CTGTTTGCCA CTGTGTGTGA GAAGACAGTT 
CTGAAGCGGG TCCTGAAGGA GCTCTGGCGG GTGGTGATGA ACACCATGGA GCGGGTTATT 
GTCCTGCCCC CACTCACTGA CCAGACGGGC ACCCAGCTGA TCTTAACTGC TGCCAAGGAG 
CTGAGCCAGC TTTCCAAACT CAAGGACCAC ATGGTACGGG AGGAAACACG GAATCTCACT 
CCAAAGCAGT GTGCTGTCCT CGACTTGGCC TTGGACACCA TCAAGCAATA CTTCCATGCA 
GGAGGCAATG GTCTGAAGAA AACCTTCCTG GAAAAGAGCC CAGACCTGCA GTCTCTGCGT 
TATGCCCTCT CTCTGTATAC ACAGACTACA GACACTCTCA TCAAGACATT TGTGCGCTCA 
CAGACTGCCC AGGTACATGA TGGGAAAGGT ATTAGGTTTA CTGCTAACGA GGACATTCGG 
CCAGAAAAGG GGGCTGGTGT GGATGACCCC GTGGGAGAAG TCTCTATTCA GGTGGACTTA 
TTTACACATC CTGGCACTGG GGAGCATAAG GTCACAGTGA AAGTGGTAGC AGCCAATGAC 
CTCAAGTGGC AGACAGCGGG TATGTTCCGG CCTTTTGTGG AAGTGACCAT GGTTGGCCCA 
CACCAAAGTG ATAAGAAGAG GAAGTTCACA ACCAAGTCTA AAAGTAACAA CTGGACTCCC 
AAGTACAACG AGACGTTTCA CTTCCTCCTG GGAAATGAAG AGGGTCCAGA GGCCTATGAA 
TTGCAGATCT GTGTGAAGGA TTACTGCTTT GCCCGGGAGG ATCGTGTGAT AGGACTGGCA 
GTGATGCCCC TGAGGGATGT GGCAGCTAAG GGCAGCTGTG CCTGCTGGTG CCCACTGGGC 
CGGAAGATCC ATATGGATGA GACAGGCATG ACCATTCTCC GGATTCTGTC TCAGAGGAGC 
AATGACGAGG TAGCCCGAGA GTTCGTGAAG CTCAAATCAG AGTCTCGATC CACAGAGGAA 
GGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537852.1
CDS	938..6886
Translation	MKRLLRESEEEIMLTLGPSSSLSPDKVRAETVCSVKSKSTGPTGSLPEDNFPLPCCDSTTSGERDGDLPQLCSFEQQANNQPSLASTACASGSDSRELSPASITNCSDPSERNQARPILPRGPGQRCRYEQQEPLGDVVEYIIRELQGISRLQSEIAELQQHLNQVQGSVDEVSSCVDSVLSEIEGLHVGSSSLGKVCSGEKAQELHVERSREEAILYLYGLPEHDGENTMELVDNFLAKHLCVNGMQCNRYIQEAYRTGTAPAPRPTVVKLVHLEHRDLILQKSILLQSVGVRVATREEPVWSEGCEDPPKESLSCLQQLQDHSWNSLNQDKPALRLETGNRKQMTEPHQIRTQNQQRGPQASEHQGPRLLPKDGLAKQSDVSKLQEEVKGTSGASWVTSDCCGELSLLHPLEGSSSVLISKEEDCEKLQIFKQGRQEHEACKVTKLQSDCSNSIKDSSCLSLSGPLKADMVNTEDKMLGCEAGLDILSPKQLEDLLADKSRRFATLSPDSTMEEVIIGPETLSNMVHIDLNEEENCTAQVLKDVFDKSSRVLADSQEDEDVEIKFHTTKLSRAIQHFRLALQGVFQKLENNGSISPEDLESNESGSQSENSDRLLWTVSSGGAHDCSVESPASQGSESLLSVVSGGVGISIQGDQTSQAPSNFSLASNNSPFANSLLSFPLAPCLGSETCSRPDSPNQGKLSLEQVCAETIYLNKCINNFKNVLREKRLRQKKLLQELVQTASHLSVEDIPSEGNQEAIQISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537789.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537789.3

ORF Insert Sequence:

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGTCACTGGT CCAGTCACGG AAAGCTGGGA TTACATCTGC TATGGCTACA 
CGTACCTCTC TCAAGGATGA AGAACTGAAA TCTCATGTGT ATAAGAAAAC TCTGCAGGCC 
TTAATCTACC CCATCTCGTG TACCACGCCC CACAACTTTG AGGTCTGGTC TGCCACTACA 
CCTACCTATT GCTATGAGTG TGAAGGCTTG CTCTGGGGCC TTGCCCGGCA AGGCATGCGC 
TGCAGCGAGT GTGGAGTCAA GTGCCATGAG AAGTGCCAGG ACCTGCTCAA TGCTGACTGC 
CTACAGCGGG CTGCTGAAAA GAGCTCTAAG CATGGAGCTG AGGACCGAAC TCAGAACATT 
ATCATGGCCA TGAAGGACCG TATGAAGATC CGAGAACGGA ATAAACCAGA GATCTTTGAG 
GTTATCCGGG ATGTGTTCAC AGTGAGCAAA GTTGCGCATG TGCAGCAGAT GAAAACAGTA 
AAGCAGAGTG TCCTGGATGG CACCTCCAAG TGGTCAGCCA AAATTACCAT CACGGTGGTT 
TGTGCCCAGG GCCTACAAGC CAAAGACAAG ACAGGATCCA GTGACCCTTA TGTGACTGTG 
CAAGTTGGGA AAACCAAGAA GCGCACCAAG ACCATTTTTG GAAATTTGAA TCCTGTTTGG 
GAAGAGAAGT TCCATTTTGA GTGCCACAAC TCTTCTGATC GAATTAAGGT ACGCGTGTGG 
GATGAGGATG ATGACATCAA GTCCAGAGTA AAGCAGCGGC TAAAGCGAGA GTCAGATGAT 
TTCCTTGGCC AAACTATCAT TGAAGTTCGA ACGCTGAGTG GAGAGATGGA TGTCTGGTAC 
AATCTGGAGA AGAGAACAGA CAAGTCAGCT GTCTCAGGGG CTATCCGACT TCAAATCAGT 
GTGGAGATCA AGGGGGAGGA GAAGGTAGCC CCATACCATG TACAATATAC ATGTCTCCAT 
GAGAACCTTT TCCATTACCT CACAGACATT CAGGGCAGTG GAGGAGTCTG GATCCCAGAG 
GCTCGGGGGG ACGATGCATG GAAGGTGTAC TTTGATGAGA CAGCCCAAGA AATTGTGGAT 
GAATTCGCTA TGCGCTATGG CATCGAGTCC ATTTATCAGG CCATGACGCA CTTTGCATGT 
TTGTCATCCA AGTACATGTG TCCTGGTGTA CCAGCCGTGA TGAGCACCTT ACTGGCCAAC 
ATCAATGCCT ATTATGCTCA CACAACTGCC TCCACTAACG TCTCTGCATC TGATCGTTTT 
GCAGCCTCCA ACTTTGGTAA AGAAAGATTT GTAAAACTGC TGGACCAGCT ACACAACTCA 
CTGAGGATAG ACCTCTCTAC ATACAGGAAT AACTTTCCTG CTGGGAGCCC TGAGCGGCTT 
CAGGATTTAA AGTCTACAGT GGATTTGCTG ACCAGCATTA CTTTCTTCAG GATGAAGGTG 
CAAGAACTTC AGAGCCCTCC AAGAGCCAGC CAGGTGGTAA AGGACTGTGT GAAAGCCTGC 
TTGAACTCTA CATATGAATA CATCTTCAAC AACTGCCATG ACCTCTACAG TCACCAGTAT 
CAGCTGCAGG AACAACCTCT AGAGGAACCA GGGCCCAGCA TTCGGAACTT GGATTTCTGG 
CCCAAACTTA TCACACTCAT TGTTTCGATC ATAGAGGAGG ATAAGAATTC CTACACACCT 
GTTCTGAGCC AGTTTCCTCA GGAGTTGAAC GTAGGAAAAG TCAGTGCAGA AGTGATGTGG 
CATCTGTTTG CTCAAGACAT GAAATATGCA CTGGAGGAAC ACGAGAAAGA CCGGCTGTGT 
AAGAGTGCTG ACTACATGAA CCTGCACTTC AAGGTGAAGT GGCTCCACAA TGAATATGTG 
CGCGATCTGC CTGCCCTCCA GGGGCAAGTG CCTGAGTACC CAGCGTGGTT TGAGCAGTTT 
GTGCTACAGT GGCTAGATGA GAATGAAGAT GTGTCACTGG AATTCCTTCG TGGGGCCTTG 
GAACGAGATA AGAAGGATGG GTTCCAGCAG ACATCAGAGC ATGCCTTGTT TTCCTGTTCT 
GTGGTGGATG TCTTTACACA ACTCAACCAG AGCTTTGAGA TCATCCGGAA GTTGGAATGT 
CCAGACCCCA ACATCCTTGC CCACTACATG AGAAGGTTTG CTAAGACCAT TGGGAAAGTG 
CTGATGCAGT ATGCAGACAT CTTATCAAAG AACTTCCCAG CTTACTGCAC AAAGGAGAGA 
CTGCCCTGTA TCCTGATGAA CAACATGCAG CAACTGAGGG TCCAGCTGGA GAAAATGTTT 
GAGGCCATGG GAGGCAAAGA GCTGGACTCC GAAGCTGCAG ACAGTCTGAA GGAGCTGCAG 
GTGAAACTGA ATACAGTTCT GGATGAGCTT AGCATGGTGT TTGGAAACAG CTTCCAGGTG 
CGGATTGATG AATGTGTTCG ACAAATGGCT GACATCCTGG GCCAAGTTCG GGGCACAGGG 
AATGCATCCC CTAACGCCAG GGCCTCGGTG GCTCAGGATG CAGATAGTGT GCTGCGACCT 
CTCATGGACT TCCTGGATGG CAACCTCACG CTGTTTGCCA CTGTGTGTGA GAAGACAGTT 
CTGAAGCGGG TCCTGAAGGA GCTCTGGCGG GTGGTGATGA ACACCATGGA GCGGGTTATT 
GTCCTGCCCC CACTCACTGA CCAGACGGGC ACCCAGCTGA TCTTAACTGC TGCCAAGGAG 
CTGAGCCAGC TTTCCAAACT CAAGGACCAC ATGGTACGGG AGGAAACACG GAATCTCACT 
CCAAAGCAGT GTGCTGTCCT CGACTTGGCC TTGGACACCA TCAAGCAATA CTTCCATGCA 
GGAGGCAATG GTCTGAAGAA AACCTTCCTG GAAAAGAGCC CAGACCTGCA GTCTCTGCGT 
TATGCCCTCT CTCTGTATAC ACAGACTACA GACACTCTCA TCAAGACATT TGTGCGCTCA 
CAGACTGCCC AGGTACATGA TGGGAAAGGT ATTAGGTTTA CTGCTAACGA GGACATTCGG 
CCAGAAAAGG GGGCTGGTGT GGATGACCCC GTGGGAGAAG TCTCTATTCA GGTGGACTTA 
TTTACACATC CTGGCACTGG GGAGCATAAG GTCACAGTGA AAGTGGTAGC AGCCAATGAC 
CTCAAGTGGC AGACAGCGGG TATGTTCCGG CCTTTTGTGG AAGTGACCAT GGTTGGCCCA 
CACCAAAGTG ATAAGAAGAG GAAGTTCACA ACCAAGTCTA AAAGTAACAA CTGGACTCCC 
AAGTACAACG AGACGTTTCA CTTCCTCCTG GGAAATGAAG AGGGTCCAGA GGCCTATGAA 
TTGCAGATCT GTGTGAAGGA TTACTGCTTT GCCCGGGAGG ATCGTGTGAT AGGACTGGCA 
GTGATGCCCC TGAGGGATGT GGCAGCTAAG GGCAGCTGTG CCTGCTGGTG CCCACTGGGC 
CGGAAGATCC ATATGGATGA GACAGGCATG ACCATTCTCC GGATTCTGTC TCAGAGGAGC 
AATGACGAGG TAGCCCGAGA GTTCGTGAAG CTCAAATCAG AGTCTCGATC CACAGAGGAA 
GGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537790.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537853.1 Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X5, mRNA.

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CloneID	OMu32855
Clone ID Related Accession (Same CDS sequence)	XM_006537790.3 , XM_006537790.4
Accession Version	XM_006537790.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537790.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGCAGGAACA ACCTCTAGAG GAACCAGGGC CCAGCATTCG GAACTTGGAT 
TTCTGGCCCA AACTTATCAC ACTCATTGTT TCGATCATAG AGGAGGATAA GAATTCCTAC 
ACACCTGTTC TGAGCCAGTT TCCTCAGGAG TTGAACGTAG GAAAAGTCAG TGCAGAAGTG 
ATGTGGCATC TGTTTGCTCA AGACATGAAA TATGCACTGG AGGAACACGA GAAAGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TATGTGCGCG ATCTGCCTGC CCTCCAGGGG CAAGTGCCTG AGTACCCAGC GTGGTTTGAG 
CAGTTTGTGC TACAGTGGCT AGATGAGAAT GAAGATGTGT CACTGGAATT CCTTCGTGGG 
GCCTTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC CTTGTTTTCC 
TGTTCTGTGG TGGATGTCTT TACACAACTC AACCAGAGCT TTGAGATCAT CCGGAAGTTG 
GAATGTCCAG ACCCCAACAT CCTTGCCCAC TACATGAGAA GGTTTGCTAA GACCATTGGG 
AAAGTGCTGA TGCAGTATGC AGACATCTTA TCAAAGAACT TCCCAGCTTA CTGCACAAAG 
GAGAGACTGC CCTGTATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAAGAGCTG GACTCCGAAG CTGCAGACAG TCTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTTAGCA TGGTGTTTGG AAACAGCTTC 
CAGGTGCGGA TTGATGAATG TGTTCGACAA ATGGCTGACA TCCTGGGCCA AGTTCGGGGC 
ACAGGGAATG CATCCCCTAA CGCCAGGGCC TCGGTGGCTC AGGATGCAGA TAGTGTGCTG 
CGACCTCTCA TGGACTTCCT GGATGGCAAC CTCACGCTGT TTGCCACTGT GTGTGAGAAG 
ACAGTTCTGA AGCGGGTCCT GAAGGAGCTC TGGCGGGTGG TGATGAACAC CATGGAGCGG 
GTTATTGTCC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT AACTGCTGCC 
AAGGAGCTGA GCCAGCTTTC CAAACTCAAG GACCACATGG TACGGGAGGA AACACGGAAT 
CTCACTCCAA AGCAGTGTGC TGTCCTCGAC TTGGCCTTGG ACACCATCAA GCAATACTTC 
CATGCAGGAG GCAATGGTCT GAAGAAAACC TTCCTGGAAA AGAGCCCAGA CCTGCAGTCT 
CTGCGTTATG CCCTCTCTCT GTATACACAG ACTACAGACA CTCTCATCAA GACATTTGTG 
CGCTCACAGA CTGCCCAGGG GGCTGGTGTG GATGACCCCG TGGGAGAAGT CTCTATTCAG 
GTGGACTTAT TTACACATCC TGGCACTGGG GAGCATAAGG TCACAGTGAA AGTGGTAGCA 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CTTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA TAAGAAGAGG AAGTTCACAA CCAAGTCTAA AAGTAACAAC 
TGGACTCCCA AGTACAACGA GACGTTTCAC TTCCTCCTGG GAAATGAAGA GGGTCCAGAG 
GCCTATGAAT TGCAGATCTG TGTGAAGGAT TACTGCTTTG CCCGGGAGGA TCGTGTGATA 
GGACTGGCAG TGATGCCCCT GAGGGATGTG GCAGCTAAGG GCAGCTGTGC CTGCTGGTGC 
CCACTGGGCC GGAAGATCCA TATGGATGAG ACAGGCATGA CCATTCTCCG GATTCTGTCT 
CAGAGGAGCA ATGACGAGGT AGCCCGAGAG TTCGTGAAGC TCAAATCAGA GTCTCGATCC 
ACAGAGGAAG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537853.1
CDS	937..6834
Translation	MKRLLRESEEEIMLTLGPSSSLSPDKVRAETVCSVKSKSTGPTGSLPEDNFPLPCCDSTTSGERDGDLPQLCSFEQQANNQPSLASTACASGSDSRELSPASITNCSDPSERNQARPILPRGPGQRCRYEQQEPLGDVVEYIIRELQGISRLQSEIAELQQHLNQVQGSVDEVSSCVDSVLSEIEGLHVGSSSLGKVCSGEKAQELHVERSREEAILYLYGLPEHDGENTMELVDNFLAKHLCVNGMQCNRYIQEAYRTGTAPAPRPTVVKLVHLEHRDLILQKSILLQSVGVRVATREEPVWSEGCEDPPKESLSCLQQLQDHSWNSLNQDKPALRLETGNRKQMTEPHQIRTQNQQRGPQASEHQGPRLLPKDGLAKQSDVSKLQEEVKGTSGASWVTSDCCGELSLLHPLEGSSSVLISKEEDCEKLQIFKQGRQEHEACKVTKLQSDCSNSIKDSSCLSLSGPLKADMVNTEDKMLGCEAGLDILSPKQLEDLLADKSRRFATLSPDSTMEEVIIGPETLSNMVHIDLNEEENCTAQVLKDVFDKSSRVLADSQEDEDVEIKFHTTKLSRAIQHFRLALQGVFQKLENNGSISPEDLESNESGSQSENSDRLLWTVSSGGAHDCSVESPASQGSESLLSVVSGGVGISIQGDQTSQAPSNFSLASNNSPFANSLLSFPLAPCLGSETCSRPDSPNQGKLSLEQVCAETIYLNKCINNFKNVLREKRLRQKKLLQELVQTASHLSVEDIPSEGNQEAIQISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537790.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537790.3

ORF Insert Sequence:

ATGAAGCGAC TGTTGAGAGA GTCTGAGGAG GAAATCATGC TGACGTTGGG ACCTTCATCC 
AGCCTCTCCC CAGATAAGGT CAGGGCAGAG ACTGTATGCA GTGTCAAGAG CAAGTCCACG 
GGTCCTACTG GATCCCTGCC AGAGGACAAC TTCCCACTCC CTTGCTGTGA CTCCACCACT 
AGTGGAGAAA GAGATGGGGA CCTGCCCCAA CTTTGTAGCT TTGAGCAGCA AGCCAATAAC 
CAACCTTCTC TGGCCTCCAC TGCCTGTGCT TCAGGATCTG ACTCTCGGGA ACTCTCACCT 
GCCAGTATAA CAAACTGTTC AGATCCTTCA GAGAGAAACC AAGCCAGGCC CATTTTGCCT 
AGGGGTCCTG GCCAGAGATG TAGGTATGAA CAGCAAGAGC CTCTGGGAGA TGTAGTAGAA 
TATATCATCA GAGAACTGCA AGGTATCAGT CGTCTTCAGT CTGAGATTGC TGAGCTGCAA 
CAGCACTTGA ACCAAGTCCA GGGCTCTGTG GATGAAGTGT CTAGCTGTGT AGATTCAGTA 
CTGAGTGAAA TAGAAGGCTT GCACGTAGGT AGCAGCTCCC TGGGCAAAGT GTGCAGTGGG 
GAAAAGGCTC AGGAACTCCA TGTAGAAAGA TCCAGAGAGG AAGCCATTCT TTATCTATAT 
GGACTTCCTG AACATGATGG GGAAAACACT ATGGAACTGG TGGACAACTT TCTAGCAAAG 
CACCTCTGTG TTAATGGCAT GCAGTGCAAC AGGTATATCC AAGAGGCATA CAGAACAGGC 
ACAGCTCCTG CCCCCCGGCC TACTGTGGTG AAACTTGTCC ATCTAGAGCA CAGAGACCTC 
ATCTTGCAAA AATCCATCCT CTTGCAGAGT GTGGGGGTCA GGGTTGCTAC CAGAGAGGAA 
CCAGTGTGGT CAGAAGGGTG TGAGGATCCC CCAAAGGAGT CTTTATCATG CTTGCAACAA 
CTTCAAGACC ACAGTTGGAA CTCTTTAAAT CAGGATAAAC CAGCTCTTCG GCTAGAAACA 
GGGAACAGAA AACAAATGAC AGAACCACAC CAGATAAGGA CTCAGAATCA ACAAAGAGGA 
CCCCAGGCTT CTGAGCATCA GGGCCCTCGT TTACTACCAA AGGATGGCTT AGCAAAACAG 
AGTGATGTGT CTAAGTTGCA GGAGGAAGTA AAAGGAACAT CAGGAGCATC CTGGGTAACC 
AGTGATTGTT GTGGTGAACT TTCTCTCCTC CATCCACTTG AGGGGTCTTC CTCAGTGTTA 
ATCTCAAAAG AGGAAGATTG TGAAAAATTA CAGATTTTCA AACAGGGCAG ACAAGAACAT 
GAGGCATGTA AGGTGACAAA GCTACAAAGT GATTGCAGCA ATTCAATTAA GGACAGCAGT 
TGCCTTTCAC TGTCTGGACC ACTGAAAGCA GATATGGTAA ACACAGAGGA CAAGATGCTA 
GGCTGTGAAG CTGGACTTGA TATTCTGAGC CCAAAGCAGC TGGAGGACCT TTTGGCAGAC 
AAGTCTAGAA GGTTTGCGAC TCTGAGTCCT GACAGCACGA TGGAGGAAGT GATCATAGGG 
CCTGAGACTT TGAGTAACAT GGTTCATATT GACCTGAATG AAGAAGAGAA CTGTACTGCC 
CAGGTTCTTA AGGATGTCTT TGATAAGTCC TCACGTGTTC TGGCTGACTC CCAGGAGGAT 
GAAGATGTAG AAATTAAATT CCACACAACT AAGCTGAGTC GAGCAATCCA GCACTTTCGT 
TTGGCTCTGC AGGGGGTATT TCAGAAGCTG GAGAACAATG GGTCCATCAG TCCTGAGGAC 
CTGGAAAGCA ACGAGAGTGG TTCCCAGTCA GAGAACAGTG ATAGACTTCT TTGGACAGTA 
AGTTCAGGAG GTGCCCACGA TTGTTCAGTG GAGAGTCCAG CAAGTCAGGG AAGTGAGAGC 
TTGCTCAGTG TGGTTTCTGG TGGAGTAGGC ATCTCTATAC AGGGTGATCA GACATCTCAA 
GCACCAAGCA ACTTCTCTCT TGCTTCTAAC AACTCACCTT TTGCAAACTC ACTGCTCAGT 
TTCCCTCTCG CCCCATGTCT GGGAAGCGAG ACTTGTTCCA GGCCTGACTC TCCTAATCAA 
GGCAAACTGA GTTTAGAACA AGTATGTGCA GAGACCATTT ACCTTAACAA ATGCATCAAC 
AATTTTAAAA ACGTTCTAAG AGAGAAAAGA CTGAGGCAGA AAAAACTTTT GCAAGAGCTA 
GTACAGACGG CAAGCCATCT TTCAGTAGAA GACATCCCCT CAGAAGGAAA TCAAGAAGCT 
ATTCAGATTT CAGATGATGG TGACCCCTCC TTGCCTCAGT GGCTCCCAGA AGGGCCAGCT 
GGAGGGCTCT ATGGCATTGA CAGCATGCCA GATCTACGAA GGAAGAAGCC ACTGCCACTT 
GTCAGTGATC TGGCTATGTC ACTGGTCCAG TCACGGAAAG CTGGGATTAC ATCTGCTATG 
GCTACACGTA CCTCTCTCAA GGATGAAGAA CTGAAATCTC ATGTGTATAA GAAAACTCTG 
CAGGCCTTAA TCTACCCCAT CTCGTGTACC ACGCCCCACA ACTTTGAGGT CTGGTCTGCC 
ACTACACCTA CCTATTGCTA TGAGTGTGAA GGCTTGCTCT GGGGCCTTGC CCGGCAAGGC 
ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC CATGAGAAGT GCCAGGACCT GCTCAATGCT 
GACTGCCTAC AGCGGGCTGC TGAAAAGAGC TCTAAGCATG GAGCTGAGGA CCGAACTCAG 
AACATTATCA TGGCCATGAA GGACCGTATG AAGATCCGAG AACGGAATAA ACCAGAGATC 
TTTGAGGTTA TCCGGGATGT GTTCACAGTG AGCAAAGTTG CGCATGTGCA GCAGATGAAA 
ACAGTAAAGC AGAGTGTCCT GGATGGCACC TCCAAGTGGT CAGCCAAAAT TACCATCACG 
GTGGTTTGTG CCCAGGGCCT ACAAGCCAAA GACAAGACAG GATCCAGTGA CCCTTATGTG 
ACTGTGCAAG TTGGGAAAAC CAAGAAGCGC ACCAAGACCA TTTTTGGAAA TTTGAATCCT 
GTTTGGGAAG AGAAGTTCCA TTTTGAGTGC CACAACTCTT CTGATCGAAT TAAGGTACGC 
GTGTGGGATG AGGATGATGA CATCAAGTCC AGAGTAAAGC AGCGGCTAAA GCGAGAGTCA 
GATGATTTCC TTGGCCAAAC TATCATTGAA GTTCGAACGC TGAGTGGAGA GATGGATGTC 
TGGTACAATC TGGAGAAGAG AACAGACAAG TCAGCTGTCT CAGGGGCTAT CCGACTTCAA 
ATCAGTGTGG AGATCAAGGG GGAGGAGAAG GTAGCCCCAT ACCATGTACA ATATACATGT 
CTCCATGAGA ACCTTTTCCA TTACCTCACA GACATTCAGG GCAGTGGAGG AGTCTGGATC 
CCAGAGGCTC GGGGGGACGA TGCATGGAAG GTGTACTTTG ATGAGACAGC CCAAGAAATT 
GTGGATGAAT TCGCTATGCG CTATGGCATC GAGTCCATTT ATCAGGCCAT GACGCACTTT 
GCATGTTTGT CATCCAAGTA CATGTGTCCT GGTGTACCAG CCGTGATGAG CACCTTACTG 
GCCAACATCA ATGCCTATTA TGCTCACACA ACTGCCTCCA CTAACGTCTC TGCATCTGAT 
CGTTTTGCAG CCTCCAACTT TGGTAAAGAA AGATTTGTAA AACTGCTGGA CCAGCTACAC 
AACTCACTGA GGATAGACCT CTCTACATAC AGGAATAACT TTCCTGCTGG GAGCCCTGAG 
CGGCTTCAGG ATTTAAAGTC TACAGTGGAT TTGCTGACCA GCATTACTTT CTTCAGGATG 
AAGGTGCAAG AACTTCAGAG CCCTCCAAGA GCCAGCCAGG TGGTAAAGGA CTGTGTGAAA 
GCCTGCTTGA ACTCTACATA TGAATACATC TTCAACAACT GCCATGACCT CTACAGTCAC 
CAGTATCAGC TGCAGGAACA ACCTCTAGAG GAACCAGGGC CCAGCATTCG GAACTTGGAT 
TTCTGGCCCA AACTTATCAC ACTCATTGTT TCGATCATAG AGGAGGATAA GAATTCCTAC 
ACACCTGTTC TGAGCCAGTT TCCTCAGGAG TTGAACGTAG GAAAAGTCAG TGCAGAAGTG 
ATGTGGCATC TGTTTGCTCA AGACATGAAA TATGCACTGG AGGAACACGA GAAAGACCGG 
CTGTGTAAGA GTGCTGACTA CATGAACCTG CACTTCAAGG TGAAGTGGCT CCACAATGAA 
TATGTGCGCG ATCTGCCTGC CCTCCAGGGG CAAGTGCCTG AGTACCCAGC GTGGTTTGAG 
CAGTTTGTGC TACAGTGGCT AGATGAGAAT GAAGATGTGT CACTGGAATT CCTTCGTGGG 
GCCTTGGAAC GAGATAAGAA GGATGGGTTC CAGCAGACAT CAGAGCATGC CTTGTTTTCC 
TGTTCTGTGG TGGATGTCTT TACACAACTC AACCAGAGCT TTGAGATCAT CCGGAAGTTG 
GAATGTCCAG ACCCCAACAT CCTTGCCCAC TACATGAGAA GGTTTGCTAA GACCATTGGG 
AAAGTGCTGA TGCAGTATGC AGACATCTTA TCAAAGAACT TCCCAGCTTA CTGCACAAAG 
GAGAGACTGC CCTGTATCCT GATGAACAAC ATGCAGCAAC TGAGGGTCCA GCTGGAGAAA 
ATGTTTGAGG CCATGGGAGG CAAAGAGCTG GACTCCGAAG CTGCAGACAG TCTGAAGGAG 
CTGCAGGTGA AACTGAATAC AGTTCTGGAT GAGCTTAGCA TGGTGTTTGG AAACAGCTTC 
CAGGTGCGGA TTGATGAATG TGTTCGACAA ATGGCTGACA TCCTGGGCCA AGTTCGGGGC 
ACAGGGAATG CATCCCCTAA CGCCAGGGCC TCGGTGGCTC AGGATGCAGA TAGTGTGCTG 
CGACCTCTCA TGGACTTCCT GGATGGCAAC CTCACGCTGT TTGCCACTGT GTGTGAGAAG 
ACAGTTCTGA AGCGGGTCCT GAAGGAGCTC TGGCGGGTGG TGATGAACAC CATGGAGCGG 
GTTATTGTCC TGCCCCCACT CACTGACCAG ACGGGCACCC AGCTGATCTT AACTGCTGCC 
AAGGAGCTGA GCCAGCTTTC CAAACTCAAG GACCACATGG TACGGGAGGA AACACGGAAT 
CTCACTCCAA AGCAGTGTGC TGTCCTCGAC TTGGCCTTGG ACACCATCAA GCAATACTTC 
CATGCAGGAG GCAATGGTCT GAAGAAAACC TTCCTGGAAA AGAGCCCAGA CCTGCAGTCT 
CTGCGTTATG CCCTCTCTCT GTATACACAG ACTACAGACA CTCTCATCAA GACATTTGTG 
CGCTCACAGA CTGCCCAGGG GGCTGGTGTG GATGACCCCG TGGGAGAAGT CTCTATTCAG 
GTGGACTTAT TTACACATCC TGGCACTGGG GAGCATAAGG TCACAGTGAA AGTGGTAGCA 
GCCAATGACC TCAAGTGGCA GACAGCGGGT ATGTTCCGGC CTTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGTGA TAAGAAGAGG AAGTTCACAA CCAAGTCTAA AAGTAACAAC 
TGGACTCCCA AGTACAACGA GACGTTTCAC TTCCTCCTGG GAAATGAAGA GGGTCCAGAG 
GCCTATGAAT TGCAGATCTG TGTGAAGGAT TACTGCTTTG CCCGGGAGGA TCGTGTGATA 
GGACTGGCAG TGATGCCCCT GAGGGATGTG GCAGCTAAGG GCAGCTGTGC CTGCTGGTGC 
CCACTGGGCC GGAAGATCCA TATGGATGAG ACAGGCATGA CCATTCTCCG GATTCTGTCT 
CAGAGGAGCA ATGACGAGGT AGCCCGAGAG TTCGTGAAGC TCAAATCAGA GTCTCGATCC 
ACAGAGGAAG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537791.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537854.1 Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X6, mRNA.

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CloneID	OMu32856
Clone ID Related Accession (Same CDS sequence)	XM_006537791.4 , XM_006537791.3
Accession Version	XM_006537791.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGAGAC 
AGTGACTACC GCAGTGAGAC CAGCAATAGC GCCCCACCTC CTTACCACAC GACTACCCAG 
CCCAACGCTT CTGTACACCA GTTCCCTGTT CCTGTGCGGC TGCCACAGCA GCTCTTTCTT 
CAAGGCAGTT CCCATGACTC TTGCAATGAC TCTATGCAGA GTTACGACCT TGATTATCCA 
GAGCGTCGGG CCCTCAGCCC TACCAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CAAGGAAGCT CTCTGCTGAG TGAGCTGGAC CAGTATCATG AGCAAGATGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGTCATAGC TATGGCAGCC TCTCCAAAGA TGGCCAGGCT 
GGTTTGGGAG AACAAGAGAA AGCCTTGGAA GTGACATGTG AATCAGAAAA AGAGAAAACA 
GGTGAATCCA AGGAGATGAG AGATGATGCT ACAATCCACC CTCCTTCAGA TCTGGTGCTG 
CATAAGGACC ATGTCCTAGG ACCCCAGGAG AGTCTTCCTG AGGAGACCGC ATCATCGCCA 
TTTACCCAGG CCAGAGCACA CTGGTTCCGA GCGGTTACCA AGGTCCGACT CCAACTGCAG 
GAGATTTCAG ATGATGGTGA CCCCTCCTTG CCTCAGTGGC TCCCAGAAGG GCCAGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGAAGGA AGAAGCCACT GCCACTTGTC 
AGTGATCTGG CTATGTCACT GGTCCAGTCA CGGAAAGCTG GGATTACATC TGCTATGGCT 
ACACGTACCT CTCTCAAGGA TGAAGAACTG AAATCTCATG TGTATAAGAA AACTCTGCAG 
GCCTTAATCT ACCCCATCTC GTGTACCACG CCCCACAACT TTGAGGTCTG GTCTGCCACT 
ACACCTACCT ATTGCTATGA GTGTGAAGGC TTGCTCTGGG GCCTTGCCCG GCAAGGCATG 
CGCTGCAGCG AGTGTGGAGT CAAGTGCCAT GAGAAGTGCC AGGACCTGCT CAATGCTGAC 
TGCCTACAGC GGGCTGCTGA AAAGAGCTCT AAGCATGGAG CTGAGGACCG AACTCAGAAC 
ATTATCATGG CCATGAAGGA CCGTATGAAG ATCCGAGAAC GGAATAAACC AGAGATCTTT 
GAGGTTATCC GGGATGTGTT CACAGTGAGC AAAGTTGCGC ATGTGCAGCA GATGAAAACA 
GTAAAGCAGA GTGTCCTGGA TGGCACCTCC AAGTGGTCAG CCAAAATTAC CATCACGGTG 
GTTTGTGCCC AGGGCCTACA AGCCAAAGAC AAGACAGGAT CCAGTGACCC TTATGTGACT 
GTGCAAGTTG GGAAAACCAA GAAGCGCACC AAGACCATTT TTGGAAATTT GAATCCTGTT 
TGGGAAGAGA AGTTCCATTT TGAGTGCCAC AACTCTTCTG ATCGAATTAA GGTACGCGTG 
TGGGATGAGG ATGATGACAT CAAGTCCAGA GTAAAGCAGC GGCTAAAGCG AGAGTCAGAT 
GATTTCCTTG GCCAAACTAT CATTGAAGTT CGAACGCTGA GTGGAGAGAT GGATGTCTGG 
TACAATCTGG AGAAGAGAAC AGACAAGTCA GCTGTCTCAG GGGCTATCCG ACTTCAAATC 
AGTGTGGAGA TCAAGGGGGA GGAGAAGGTA GCCCCATACC ATGTACAATA TACATGTCTC 
CATGAGAACC TTTTCCATTA CCTCACAGAC ATTCAGGGCA GTGGAGGAGT CTGGATCCCA 
GAGGCTCGGG GGGACGATGC ATGGAAGGTG TACTTTGATG AGACAGCCCA AGAAATTGTG 
GATGAATTCG CTATGCGCTA TGGCATCGAG TCCATTTATC AGGCCATGAC GCACTTTGCA 
TGTTTGTCAT CCAAGTACAT GTGTCCTGGT GTACCAGCCG TGATGAGCAC CTTACTGGCC 
AACATCAATG CCTATTATGC TCACACAACT GCCTCCACTA ACGTCTCTGC ATCTGATCGT 
TTTGCAGCCT CCAACTTTGG TAAAGAAAGA TTTGTAAAAC TGCTGGACCA GCTACACAAC 
TCACTGAGGA TAGACCTCTC TACATACAGG AATAACTTTC CTGCTGGGAG CCCTGAGCGG 
CTTCAGGATT TAAAGTCTAC AGTGGATTTG CTGACCAGCA TTACTTTCTT CAGGATGAAG 
GTGCAAGAAC TTCAGAGCCC TCCAAGAGCC AGCCAGGTGG TAAAGGACTG TGTGAAAGCC 
TGCTTGAACT CTACATATGA ATACATCTTC AACAACTGCC ATGACCTCTA CAGTCACCAG 
TATCAGCTGC AGGAACAACC TCTAGAGGAA CCAGGGCCCA GCATTCGGAA CTTGGATTTC 
TGGCCCAAAC TTATCACACT CATTGTTTCG ATCATAGAGG AGGATAAGAA TTCCTACACA 
CCTGTTCTGA GCCAGTTTCC TCAGGAGTTG AACGTAGGAA AAGTCAGTGC AGAAGTGATG 
TGGCATCTGT TTGCTCAAGA CATGAAATAT GCACTGGAGG AACACGAGAA AGACCGGCTG 
TGTAAGAGTG CTGACTACAT GAACCTGCAC TTCAAGGTGA AGTGGCTCCA CAATGAATAT 
GTGCGCGATC TGCCTGCCCT CCAGGGGCAA GTGCCTGAGT ACCCAGCGTG GTTTGAGCAG 
TTTGTGCTAC AGTGGCTAGA TGAGAATGAA GATGTGTCAC TGGAATTCCT TCGTGGGGCC 
TTGGAACGAG ATAAGAAGGA TGGGTTCCAG CAGACATCAG AGCATGCCTT GTTTTCCTGT 
TCTGTGGTGG ATGTCTTTAC ACAACTCAAC CAGAGCTTTG AGATCATCCG GAAGTTGGAA 
TGTCCAGACC CCAACATCCT TGCCCACTAC ATGAGAAGGT TTGCTAAGAC CATTGGGAAA 
GTGCTGATGC AGTATGCAGA CATCTTATCA AAGAACTTCC CAGCTTACTG CACAAAGGAG 
AGACTGCCCT GTATCCTGAT GAACAACATG CAGCAACTGA GGGTCCAGCT GGAGAAAATG 
TTTGAGGCCA TGGGAGGCAA AGAGCTGGAC TCCGAAGCTG CAGACAGTCT GAAGGAGCTG 
CAGGTGAAAC TGAATACAGT TCTGGATGAG CTTAGCATGG TGTTTGGAAA CAGCTTCCAG 
GTGCGGATTG ATGAATGTGT TCGACAAATG GCTGACATCC TGGGCCAAGT TCGGGGCACA 
GGGAATGCAT CCCCTAACGC CAGGGCCTCG GTGGCTCAGG ATGCAGATAG TGTGCTGCGA 
CCTCTCATGG ACTTCCTGGA TGGCAACCTC ACGCTGTTTG CCACTGTGTG TGAGAAGACA 
GTTCTGAAGC GGGTCCTGAA GGAGCTCTGG CGGGTGGTGA TGAACACCAT GGAGCGGGTT 
ATTGTCCTGC CCCCACTCAC TGACCAGACG GGCACCCAGC TGATCTTAAC TGCTGCCAAG 
GAGCTGAGCC AGCTTTCCAA ACTCAAGGAC CACATGGTAC GGGAGGAAAC ACGGAATCTC 
ACTCCAAAGC AGTGTGCTGT CCTCGACTTG GCCTTGGACA CCATCAAGCA ATACTTCCAT 
GCAGGAGGCA ATGGTCTGAA GAAAACCTTC CTGGAAAAGA GCCCAGACCT GCAGTCTCTG 
CGTTATGCCC TCTCTCTGTA TACACAGACT ACAGACACTC TCATCAAGAC ATTTGTGCGC 
TCACAGACTG CCCAGGTACA TGATGGGAAA GGTATTAGGT TTACTGCTAA CGAGGACATT 
CGGCCAGAAA AGGGGGCTGG TGTGGATGAC CCCGTGGGAG AAGTCTCTAT TCAGGTGGAC 
TTATTTACAC ATCCTGGCAC TGGGGAGCAT AAGGTCACAG TGAAAGTGGT AGCAGCCAAT 
GACCTCAAGT GGCAGACAGC GGGTATGTTC CGGCCTTTTG TGGAAGTGAC CATGGTTGGC 
CCACACCAAA GTGATAAGAA GAGGAAGTTC ACAACCAAGT CTAAAAGTAA CAACTGGACT 
CCCAAGTACA ACGAGACGTT TCACTTCCTC CTGGGAAATG AAGAGGGTCC AGAGGCCTAT 
GAATTGCAGA TCTGTGTGAA GGATTACTGC TTTGCCCGGG AGGATCGTGT GATAGGACTG 
GCAGTGATGC CCCTGAGGGA TGTGGCAGCT AAGGGCAGCT GTGCCTGCTG GTGCCCACTG 
GGCCGGAAGA TCCATATGGA TGAGACAGGC ATGACCATTC TCCGGATTCT GTCTCAGAGG 
AGCAATGACG AGGTAGCCCG AGAGTTCGTG AAGCTCAAAT CAGAGTCTCG ATCCACAGAG 
GAAGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537854.1
CDS	336..5207
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCCHWTYLGWGEHQTFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537791.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537791.3

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGAGAC 
AGTGACTACC GCAGTGAGAC CAGCAATAGC GCCCCACCTC CTTACCACAC GACTACCCAG 
CCCAACGCTT CTGTACACCA GTTCCCTGTT CCTGTGCGGC TGCCACAGCA GCTCTTTCTT 
CAAGGCAGTT CCCATGACTC TTGCAATGAC TCTATGCAGA GTTACGACCT TGATTATCCA 
GAGCGTCGGG CCCTCAGCCC TACCAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CAAGGAAGCT CTCTGCTGAG TGAGCTGGAC CAGTATCATG AGCAAGATGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGTCATAGC TATGGCAGCC TCTCCAAAGA TGGCCAGGCT 
GGTTTGGGAG AACAAGAGAA AGCCTTGGAA GTGACATGTG AATCAGAAAA AGAGAAAACA 
GGTGAATCCA AGGAGATGAG AGATGATGCT ACAATCCACC CTCCTTCAGA TCTGGTGCTG 
CATAAGGACC ATGTCCTAGG ACCCCAGGAG AGTCTTCCTG AGGAGACCGC ATCATCGCCA 
TTTACCCAGG CCAGAGCACA CTGGTTCCGA GCGGTTACCA AGGTCCGACT CCAACTGCAG 
GAGATTTCAG ATGATGGTGA CCCCTCCTTG CCTCAGTGGC TCCCAGAAGG GCCAGCTGGA 
GGGCTCTATG GCATTGACAG CATGCCAGAT CTACGAAGGA AGAAGCCACT GCCACTTGTC 
AGTGATCTGG CTATGTCACT GGTCCAGTCA CGGAAAGCTG GGATTACATC TGCTATGGCT 
ACACGTACCT CTCTCAAGGA TGAAGAACTG AAATCTCATG TGTATAAGAA AACTCTGCAG 
GCCTTAATCT ACCCCATCTC GTGTACCACG CCCCACAACT TTGAGGTCTG GTCTGCCACT 
ACACCTACCT ATTGCTATGA GTGTGAAGGC TTGCTCTGGG GCCTTGCCCG GCAAGGCATG 
CGCTGCAGCG AGTGTGGAGT CAAGTGCCAT GAGAAGTGCC AGGACCTGCT CAATGCTGAC 
TGCCTACAGC GGGCTGCTGA AAAGAGCTCT AAGCATGGAG CTGAGGACCG AACTCAGAAC 
ATTATCATGG CCATGAAGGA CCGTATGAAG ATCCGAGAAC GGAATAAACC AGAGATCTTT 
GAGGTTATCC GGGATGTGTT CACAGTGAGC AAAGTTGCGC ATGTGCAGCA GATGAAAACA 
GTAAAGCAGA GTGTCCTGGA TGGCACCTCC AAGTGGTCAG CCAAAATTAC CATCACGGTG 
GTTTGTGCCC AGGGCCTACA AGCCAAAGAC AAGACAGGAT CCAGTGACCC TTATGTGACT 
GTGCAAGTTG GGAAAACCAA GAAGCGCACC AAGACCATTT TTGGAAATTT GAATCCTGTT 
TGGGAAGAGA AGTTCCATTT TGAGTGCCAC AACTCTTCTG ATCGAATTAA GGTACGCGTG 
TGGGATGAGG ATGATGACAT CAAGTCCAGA GTAAAGCAGC GGCTAAAGCG AGAGTCAGAT 
GATTTCCTTG GCCAAACTAT CATTGAAGTT CGAACGCTGA GTGGAGAGAT GGATGTCTGG 
TACAATCTGG AGAAGAGAAC AGACAAGTCA GCTGTCTCAG GGGCTATCCG ACTTCAAATC 
AGTGTGGAGA TCAAGGGGGA GGAGAAGGTA GCCCCATACC ATGTACAATA TACATGTCTC 
CATGAGAACC TTTTCCATTA CCTCACAGAC ATTCAGGGCA GTGGAGGAGT CTGGATCCCA 
GAGGCTCGGG GGGACGATGC ATGGAAGGTG TACTTTGATG AGACAGCCCA AGAAATTGTG 
GATGAATTCG CTATGCGCTA TGGCATCGAG TCCATTTATC AGGCCATGAC GCACTTTGCA 
TGTTTGTCAT CCAAGTACAT GTGTCCTGGT GTACCAGCCG TGATGAGCAC CTTACTGGCC 
AACATCAATG CCTATTATGC TCACACAACT GCCTCCACTA ACGTCTCTGC ATCTGATCGT 
TTTGCAGCCT CCAACTTTGG TAAAGAAAGA TTTGTAAAAC TGCTGGACCA GCTACACAAC 
TCACTGAGGA TAGACCTCTC TACATACAGG AATAACTTTC CTGCTGGGAG CCCTGAGCGG 
CTTCAGGATT TAAAGTCTAC AGTGGATTTG CTGACCAGCA TTACTTTCTT CAGGATGAAG 
GTGCAAGAAC TTCAGAGCCC TCCAAGAGCC AGCCAGGTGG TAAAGGACTG TGTGAAAGCC 
TGCTTGAACT CTACATATGA ATACATCTTC AACAACTGCC ATGACCTCTA CAGTCACCAG 
TATCAGCTGC AGGAACAACC TCTAGAGGAA CCAGGGCCCA GCATTCGGAA CTTGGATTTC 
TGGCCCAAAC TTATCACACT CATTGTTTCG ATCATAGAGG AGGATAAGAA TTCCTACACA 
CCTGTTCTGA GCCAGTTTCC TCAGGAGTTG AACGTAGGAA AAGTCAGTGC AGAAGTGATG 
TGGCATCTGT TTGCTCAAGA CATGAAATAT GCACTGGAGG AACACGAGAA AGACCGGCTG 
TGTAAGAGTG CTGACTACAT GAACCTGCAC TTCAAGGTGA AGTGGCTCCA CAATGAATAT 
GTGCGCGATC TGCCTGCCCT CCAGGGGCAA GTGCCTGAGT ACCCAGCGTG GTTTGAGCAG 
TTTGTGCTAC AGTGGCTAGA TGAGAATGAA GATGTGTCAC TGGAATTCCT TCGTGGGGCC 
TTGGAACGAG ATAAGAAGGA TGGGTTCCAG CAGACATCAG AGCATGCCTT GTTTTCCTGT 
TCTGTGGTGG ATGTCTTTAC ACAACTCAAC CAGAGCTTTG AGATCATCCG GAAGTTGGAA 
TGTCCAGACC CCAACATCCT TGCCCACTAC ATGAGAAGGT TTGCTAAGAC CATTGGGAAA 
GTGCTGATGC AGTATGCAGA CATCTTATCA AAGAACTTCC CAGCTTACTG CACAAAGGAG 
AGACTGCCCT GTATCCTGAT GAACAACATG CAGCAACTGA GGGTCCAGCT GGAGAAAATG 
TTTGAGGCCA TGGGAGGCAA AGAGCTGGAC TCCGAAGCTG CAGACAGTCT GAAGGAGCTG 
CAGGTGAAAC TGAATACAGT TCTGGATGAG CTTAGCATGG TGTTTGGAAA CAGCTTCCAG 
GTGCGGATTG ATGAATGTGT TCGACAAATG GCTGACATCC TGGGCCAAGT TCGGGGCACA 
GGGAATGCAT CCCCTAACGC CAGGGCCTCG GTGGCTCAGG ATGCAGATAG TGTGCTGCGA 
CCTCTCATGG ACTTCCTGGA TGGCAACCTC ACGCTGTTTG CCACTGTGTG TGAGAAGACA 
GTTCTGAAGC GGGTCCTGAA GGAGCTCTGG CGGGTGGTGA TGAACACCAT GGAGCGGGTT 
ATTGTCCTGC CCCCACTCAC TGACCAGACG GGCACCCAGC TGATCTTAAC TGCTGCCAAG 
GAGCTGAGCC AGCTTTCCAA ACTCAAGGAC CACATGGTAC GGGAGGAAAC ACGGAATCTC 
ACTCCAAAGC AGTGTGCTGT CCTCGACTTG GCCTTGGACA CCATCAAGCA ATACTTCCAT 
GCAGGAGGCA ATGGTCTGAA GAAAACCTTC CTGGAAAAGA GCCCAGACCT GCAGTCTCTG 
CGTTATGCCC TCTCTCTGTA TACACAGACT ACAGACACTC TCATCAAGAC ATTTGTGCGC 
TCACAGACTG CCCAGGTACA TGATGGGAAA GGTATTAGGT TTACTGCTAA CGAGGACATT 
CGGCCAGAAA AGGGGGCTGG TGTGGATGAC CCCGTGGGAG AAGTCTCTAT TCAGGTGGAC 
TTATTTACAC ATCCTGGCAC TGGGGAGCAT AAGGTCACAG TGAAAGTGGT AGCAGCCAAT 
GACCTCAAGT GGCAGACAGC GGGTATGTTC CGGCCTTTTG TGGAAGTGAC CATGGTTGGC 
CCACACCAAA GTGATAAGAA GAGGAAGTTC ACAACCAAGT CTAAAAGTAA CAACTGGACT 
CCCAAGTACA ACGAGACGTT TCACTTCCTC CTGGGAAATG AAGAGGGTCC AGAGGCCTAT 
GAATTGCAGA TCTGTGTGAA GGATTACTGC TTTGCCCGGG AGGATCGTGT GATAGGACTG 
GCAGTGATGC CCCTGAGGGA TGTGGCAGCT AAGGGCAGCT GTGCCTGCTG GTGCCCACTG 
GGCCGGAAGA TCCATATGGA TGAGACAGGC ATGACCATTC TCCGGATTCT GTCTCAGAGG 
AGCAATGACG AGGTAGCCCG AGAGTTCGTG AAGCTCAAAT CAGAGTCTCG ATCCACAGAG 
GAAGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537794.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537857.1 Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X8, mRNA.

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CloneID	OMu32859
Clone ID Related Accession (Same CDS sequence)	XM_006537794.4 , XM_006537794.3
Accession Version	XM_006537794.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537794.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTCCTT TGAAGACCCT 
GATAGTGCCG TTGATGACCG AGACAGTGAC TACCGCAGTG AGACCAGCAA TAGCGCCCCA 
CCTCCTTACC ACACGACTAC CCAGCCCAAC GCTTCTGTAC ACCAGTTCCC TGTTCCTGTG 
CGGCTGCCAC AGCAGCTCTT TCTTCAAGGC AGTTCCCATG ACTCTTGCAA TGACTCTATG 
CAGAGTTACG ACCTTGATTA TCCAGAGCGT CGGGCCCTCA GCCCTACCAG CAGTAGTAGG 
TATGGCTCCT CCTGTAATGT GAGTCAAGGA AGCTCTCTGC TGAGTGAGCT GGACCAGTAT 
CATGAGCAAG ATGATGATGG TCGGGAGAGG GACTCCATTC ATTCTAGTCA TAGCTATGGC 
AGCCTCTCCA AAGATGGCCA GGCTGGTTTG GGAGAACAAG AGAAAGCCTT GGAAGTGACA 
TGTGAATCAG AAAAAGAGAA AACAGGTGAA TCCAAGGAGA TGAGAGATGA TGCTACAATC 
CACCCTCCTT CAGATCTGGT GCTGCATAAG GACCATGTCC TAGGACCCCA GGAGAGTCTT 
CCTGAGGAGA CCGCATCATC GCCATTTACC CAGGCCAGAG CACACTGGTT CCGAGCGGTT 
ACCAAGGTCC GACTCCAACT GCAGGAGATT TCAGATGATG GTGACCCCTC CTTGCCTCAG 
TGGCTCCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGA 
AGGAAGAAGC CACTGCCACT TGTCAGTGAT CTGGCTATGT CACTGGTCCA GTCACGGAAA 
GCTGGGATTA CATCTGCTAT GGCTACACGT ACCTCTCTCA AGGATGAAGA ACTGAAATCT 
CATGTGTATA AGAAAACTCT GCAGGCCTTA ATCTACCCCA TCTCGTGTAC CACGCCCCAC 
AACTTTGAGG TCTGGTCTGC CACTACACCT ACCTATTGCT ATGAGTGTGA AGGCTTGCTC 
TGGGGCCTTG CCCGGCAAGG CATGCGCTGC AGCGAGTGTG GAGTCAAGTG CCATGAGAAG 
TGCCAGGACC TGCTCAATGC TGACTGCCTA CAGCGGGCTG CTGAAAAGAG CTCTAAGCAT 
GGAGCTGAGG ACCGAACTCA GAACATTATC ATGGCCATGA AGGACCGTAT GAAGATCCGA 
GAACGGAATA AACCAGAGAT CTTTGAGGTT ATCCGGGATG TGTTCACAGT GAGCAAAGTT 
GCGCATGTGC AGCAGATGAA AACAGTAAAG CAGAGTGTCC TGGATGGCAC CTCCAAGTGG 
TCAGCCAAAA TTACCATCAC GGTGGTTTGT GCCCAGGGCC TACAAGCCAA AGACAAGACA 
GGATCCAGTG ACCCTTATGT GACTGTGCAA GTTGGGAAAA CCAAGAAGCG CACCAAGACC 
ATTTTTGGAA ATTTGAATCC TGTTTGGGAA GAGAAGTTCC ATTTTGAGTG CCACAACTCT 
TCTGATCGAA TTAAGGTACG CGTGTGGGAT GAGGATGATG ACATCAAGTC CAGAGTAAAG 
CAGCGGCTAA AGCGAGAGTC AGATGATTTC CTTGGCCAAA CTATCATTGA AGTTCGAACG 
CTGAGTGGAG AGATGGATGT CTGGTACAAT CTGGAGAAGA GAACAGACAA GTCAGCTGTC 
TCAGGGGCTA TCCGACTTCA AATCAGTGTG GAGATCAAGG GGGAGGAGAA GGTAGCCCCA 
TACCATGTAC AATATACATG TCTCCATGAG AACCTTTTCC ATTACCTCAC AGACATTCAG 
GGCAGTGGAG GAGTCTGGAT CCCAGAGGCT CGGGGGGACG ATGCATGGAA GGTGTACTTT 
GATGAGACAG CCCAAGAAAT TGTGGATGAA TTCGCTATGC GCTATGGCAT CGAGTCCATT 
TATCAGGCCA TGACGCACTT TGCATGTTTG TCATCCAAGT ACATGTGTCC TGGTGTACCA 
GCCGTGATGA GCACCTTACT GGCCAACATC AATGCCTATT ATGCTCACAC AACTGCCTCC 
ACTAACGTCT CTGCATCTGA TCGTTTTGCA GCCTCCAACT TTGGTAAAGA AAGATTTGTA 
AAACTGCTGG ACCAGCTACA CAACTCACTG AGGATAGACC TCTCTACATA CAGGAATAAC 
TTTCCTGCTG GGAGCCCTGA GCGGCTTCAG GATTTAAAGT CTACAGTGGA TTTGCTGACC 
AGCATTACTT TCTTCAGGAT GAAGGTGCAA GAACTTCAGA GCCCTCCAAG AGCCAGCCAG 
GTGGTAAAGG ACTGTGTGAA AGCCTGCTTG AACTCTACAT ATGAATACAT CTTCAACAAC 
TGCCATGACC TCTACAGTCA CCAGTATCAG CTGCAGGAAC AACCTCTAGA GGAACCAGGG 
CCCAGCATTC GGAACTTGGA TTTCTGGCCC AAACTTATCA CACTCATTGT TTCGATCATA 
GAGGAGGATA AGAATTCCTA CACACCTGTT CTGAGCCAGT TTCCTCAGGA GTTGAACGTA 
GGAAAAGTCA GTGCAGAAGT GATGTGGCAT CTGTTTGCTC AAGACATGAA ATATGCACTG 
GAGGAACACG AGAAAGACCG GCTGTGTAAG AGTGCTGACT ACATGAACCT GCACTTCAAG 
GTGAAGTGGC TCCACAATGA ATATGTGCGC GATCTGCCTG CCCTCCAGGG GCAAGTGCCT 
GAGTACCCAG CGTGGTTTGA GCAGTTTGTG CTACAGTGGC TAGATGAGAA TGAAGATGTG 
TCACTGGAAT TCCTTCGTGG GGCCTTGGAA CGAGATAAGA AGGATGGGTT CCAGCAGACA 
TCAGAGCATG CCTTGTTTTC CTGTTCTGTG GTGGATGTCT TTACACAACT CAACCAGAGC 
TTTGAGATCA TCCGGAAGTT GGAATGTCCA GACCCCAACA TCCTTGCCCA CTACATGAGA 
AGGTTTGCTA AGACCATTGG GAAAGTGCTG ATGCAGTATG CAGACATCTT ATCAAAGAAC 
TTCCCAGCTT ACTGCACAAA GGAGAGACTG CCCTGTATCC TGATGAACAA CATGCAGCAA 
CTGAGGGTCC AGCTGGAGAA AATGTTTGAG GCCATGGGAG GCAAAGAGCT GGACTCCGAA 
GCTGCAGACA GTCTGAAGGA GCTGCAGGTG AAACTGAATA CAGTTCTGGA TGAGCTTAGC 
ATGGTGTTTG GAAACAGCTT CCAGGTGCGG ATTGATGAAT GTGTTCGACA AATGGCTGAC 
ATCCTGGGCC AAGTTCGGGG CACAGGGAAT GCATCCCCTA ACGCCAGGGC CTCGGTGGCT 
CAGGATGCAG ATAGTGTGCT GCGACCTCTC ATGGACTTCC TGGATGGCAA CCTCACGCTG 
TTTGCCACTG TGTGTGAGAA GACAGTTCTG AAGCGGGTCC TGAAGGAGCT CTGGCGGGTG 
GTGATGAACA CCATGGAGCG GGTTATTGTC CTGCCCCCAC TCACTGACCA GACGGGCACC 
CAGCTGATCT TAACTGCTGC CAAGGAGCTG AGCCAGCTTT CCAAACTCAA GGACCACATG 
GTACGGGAGG AAACACGGAA TCTCACTCCA AAGCAGTGTG CTGTCCTCGA CTTGGCCTTG 
GACACCATCA AGCAATACTT CCATGCAGGA GGCAATGGTC TGAAGAAAAC CTTCCTGGAA 
AAGAGCCCAG ACCTGCAGTC TCTGCGTTAT GCCCTCTCTC TGTATACACA GACTACAGAC 
ACTCTCATCA AGACATTTGT GCGCTCACAG ACTGCCCAGG TACATGATGG GAAAGGTATT 
AGGTTTACTG CTAACGAGGA CATTCGGCCA GAAAAGGGGG CTGGTGTGGA TGACCCCGTG 
GGAGAAGTCT CTATTCAGGT GGACTTATTT ACACATCCTG GCACTGGGGA GCATAAGGTC 
ACAGTGAAAG TGGTAGCAGC CAATGACCTC AAGTGGCAGA CAGCGGGTAT GTTCCGGCCT 
TTTGTGGAAG TGACCATGGT TGGCCCACAC CAAAGTGATA AGAAGAGGAA GTTCACAACC 
AAGTCTAAAA GTAACAACTG GACTCCCAAG TACAACGAGA CGTTTCACTT CCTCCTGGGA 
AATGAAGAGG GTCCAGAGGC CTATGAATTG CAGATCTGTG TGAAGGATTA CTGCTTTGCC 
CGGGAGGATC GTGTGATAGG ACTGGCAGTG ATGCCCCTGA GGGATGTGGC AGCTAAGGGC 
AGCTGTGCCT GCTGGTGCCC ACTGGGCCGG AAGATCCATA TGGATGAGAC AGGCATGACC 
ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT GACGAGGTAG CCCGAGAGTT CGTGAAGCTC 
AAATCAGAGT CTCGATCCAC AGAGGAAGGG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537857.1
CDS	336..5171
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537794.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537794.3

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTCCTT TGAAGACCCT 
GATAGTGCCG TTGATGACCG AGACAGTGAC TACCGCAGTG AGACCAGCAA TAGCGCCCCA 
CCTCCTTACC ACACGACTAC CCAGCCCAAC GCTTCTGTAC ACCAGTTCCC TGTTCCTGTG 
CGGCTGCCAC AGCAGCTCTT TCTTCAAGGC AGTTCCCATG ACTCTTGCAA TGACTCTATG 
CAGAGTTACG ACCTTGATTA TCCAGAGCGT CGGGCCCTCA GCCCTACCAG CAGTAGTAGG 
TATGGCTCCT CCTGTAATGT GAGTCAAGGA AGCTCTCTGC TGAGTGAGCT GGACCAGTAT 
CATGAGCAAG ATGATGATGG TCGGGAGAGG GACTCCATTC ATTCTAGTCA TAGCTATGGC 
AGCCTCTCCA AAGATGGCCA GGCTGGTTTG GGAGAACAAG AGAAAGCCTT GGAAGTGACA 
TGTGAATCAG AAAAAGAGAA AACAGGTGAA TCCAAGGAGA TGAGAGATGA TGCTACAATC 
CACCCTCCTT CAGATCTGGT GCTGCATAAG GACCATGTCC TAGGACCCCA GGAGAGTCTT 
CCTGAGGAGA CCGCATCATC GCCATTTACC CAGGCCAGAG CACACTGGTT CCGAGCGGTT 
ACCAAGGTCC GACTCCAACT GCAGGAGATT TCAGATGATG GTGACCCCTC CTTGCCTCAG 
TGGCTCCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGA 
AGGAAGAAGC CACTGCCACT TGTCAGTGAT CTGGCTATGT CACTGGTCCA GTCACGGAAA 
GCTGGGATTA CATCTGCTAT GGCTACACGT ACCTCTCTCA AGGATGAAGA ACTGAAATCT 
CATGTGTATA AGAAAACTCT GCAGGCCTTA ATCTACCCCA TCTCGTGTAC CACGCCCCAC 
AACTTTGAGG TCTGGTCTGC CACTACACCT ACCTATTGCT ATGAGTGTGA AGGCTTGCTC 
TGGGGCCTTG CCCGGCAAGG CATGCGCTGC AGCGAGTGTG GAGTCAAGTG CCATGAGAAG 
TGCCAGGACC TGCTCAATGC TGACTGCCTA CAGCGGGCTG CTGAAAAGAG CTCTAAGCAT 
GGAGCTGAGG ACCGAACTCA GAACATTATC ATGGCCATGA AGGACCGTAT GAAGATCCGA 
GAACGGAATA AACCAGAGAT CTTTGAGGTT ATCCGGGATG TGTTCACAGT GAGCAAAGTT 
GCGCATGTGC AGCAGATGAA AACAGTAAAG CAGAGTGTCC TGGATGGCAC CTCCAAGTGG 
TCAGCCAAAA TTACCATCAC GGTGGTTTGT GCCCAGGGCC TACAAGCCAA AGACAAGACA 
GGATCCAGTG ACCCTTATGT GACTGTGCAA GTTGGGAAAA CCAAGAAGCG CACCAAGACC 
ATTTTTGGAA ATTTGAATCC TGTTTGGGAA GAGAAGTTCC ATTTTGAGTG CCACAACTCT 
TCTGATCGAA TTAAGGTACG CGTGTGGGAT GAGGATGATG ACATCAAGTC CAGAGTAAAG 
CAGCGGCTAA AGCGAGAGTC AGATGATTTC CTTGGCCAAA CTATCATTGA AGTTCGAACG 
CTGAGTGGAG AGATGGATGT CTGGTACAAT CTGGAGAAGA GAACAGACAA GTCAGCTGTC 
TCAGGGGCTA TCCGACTTCA AATCAGTGTG GAGATCAAGG GGGAGGAGAA GGTAGCCCCA 
TACCATGTAC AATATACATG TCTCCATGAG AACCTTTTCC ATTACCTCAC AGACATTCAG 
GGCAGTGGAG GAGTCTGGAT CCCAGAGGCT CGGGGGGACG ATGCATGGAA GGTGTACTTT 
GATGAGACAG CCCAAGAAAT TGTGGATGAA TTCGCTATGC GCTATGGCAT CGAGTCCATT 
TATCAGGCCA TGACGCACTT TGCATGTTTG TCATCCAAGT ACATGTGTCC TGGTGTACCA 
GCCGTGATGA GCACCTTACT GGCCAACATC AATGCCTATT ATGCTCACAC AACTGCCTCC 
ACTAACGTCT CTGCATCTGA TCGTTTTGCA GCCTCCAACT TTGGTAAAGA AAGATTTGTA 
AAACTGCTGG ACCAGCTACA CAACTCACTG AGGATAGACC TCTCTACATA CAGGAATAAC 
TTTCCTGCTG GGAGCCCTGA GCGGCTTCAG GATTTAAAGT CTACAGTGGA TTTGCTGACC 
AGCATTACTT TCTTCAGGAT GAAGGTGCAA GAACTTCAGA GCCCTCCAAG AGCCAGCCAG 
GTGGTAAAGG ACTGTGTGAA AGCCTGCTTG AACTCTACAT ATGAATACAT CTTCAACAAC 
TGCCATGACC TCTACAGTCA CCAGTATCAG CTGCAGGAAC AACCTCTAGA GGAACCAGGG 
CCCAGCATTC GGAACTTGGA TTTCTGGCCC AAACTTATCA CACTCATTGT TTCGATCATA 
GAGGAGGATA AGAATTCCTA CACACCTGTT CTGAGCCAGT TTCCTCAGGA GTTGAACGTA 
GGAAAAGTCA GTGCAGAAGT GATGTGGCAT CTGTTTGCTC AAGACATGAA ATATGCACTG 
GAGGAACACG AGAAAGACCG GCTGTGTAAG AGTGCTGACT ACATGAACCT GCACTTCAAG 
GTGAAGTGGC TCCACAATGA ATATGTGCGC GATCTGCCTG CCCTCCAGGG GCAAGTGCCT 
GAGTACCCAG CGTGGTTTGA GCAGTTTGTG CTACAGTGGC TAGATGAGAA TGAAGATGTG 
TCACTGGAAT TCCTTCGTGG GGCCTTGGAA CGAGATAAGA AGGATGGGTT CCAGCAGACA 
TCAGAGCATG CCTTGTTTTC CTGTTCTGTG GTGGATGTCT TTACACAACT CAACCAGAGC 
TTTGAGATCA TCCGGAAGTT GGAATGTCCA GACCCCAACA TCCTTGCCCA CTACATGAGA 
AGGTTTGCTA AGACCATTGG GAAAGTGCTG ATGCAGTATG CAGACATCTT ATCAAAGAAC 
TTCCCAGCTT ACTGCACAAA GGAGAGACTG CCCTGTATCC TGATGAACAA CATGCAGCAA 
CTGAGGGTCC AGCTGGAGAA AATGTTTGAG GCCATGGGAG GCAAAGAGCT GGACTCCGAA 
GCTGCAGACA GTCTGAAGGA GCTGCAGGTG AAACTGAATA CAGTTCTGGA TGAGCTTAGC 
ATGGTGTTTG GAAACAGCTT CCAGGTGCGG ATTGATGAAT GTGTTCGACA AATGGCTGAC 
ATCCTGGGCC AAGTTCGGGG CACAGGGAAT GCATCCCCTA ACGCCAGGGC CTCGGTGGCT 
CAGGATGCAG ATAGTGTGCT GCGACCTCTC ATGGACTTCC TGGATGGCAA CCTCACGCTG 
TTTGCCACTG TGTGTGAGAA GACAGTTCTG AAGCGGGTCC TGAAGGAGCT CTGGCGGGTG 
GTGATGAACA CCATGGAGCG GGTTATTGTC CTGCCCCCAC TCACTGACCA GACGGGCACC 
CAGCTGATCT TAACTGCTGC CAAGGAGCTG AGCCAGCTTT CCAAACTCAA GGACCACATG 
GTACGGGAGG AAACACGGAA TCTCACTCCA AAGCAGTGTG CTGTCCTCGA CTTGGCCTTG 
GACACCATCA AGCAATACTT CCATGCAGGA GGCAATGGTC TGAAGAAAAC CTTCCTGGAA 
AAGAGCCCAG ACCTGCAGTC TCTGCGTTAT GCCCTCTCTC TGTATACACA GACTACAGAC 
ACTCTCATCA AGACATTTGT GCGCTCACAG ACTGCCCAGG TACATGATGG GAAAGGTATT 
AGGTTTACTG CTAACGAGGA CATTCGGCCA GAAAAGGGGG CTGGTGTGGA TGACCCCGTG 
GGAGAAGTCT CTATTCAGGT GGACTTATTT ACACATCCTG GCACTGGGGA GCATAAGGTC 
ACAGTGAAAG TGGTAGCAGC CAATGACCTC AAGTGGCAGA CAGCGGGTAT GTTCCGGCCT 
TTTGTGGAAG TGACCATGGT TGGCCCACAC CAAAGTGATA AGAAGAGGAA GTTCACAACC 
AAGTCTAAAA GTAACAACTG GACTCCCAAG TACAACGAGA CGTTTCACTT CCTCCTGGGA 
AATGAAGAGG GTCCAGAGGC CTATGAATTG CAGATCTGTG TGAAGGATTA CTGCTTTGCC 
CGGGAGGATC GTGTGATAGG ACTGGCAGTG ATGCCCCTGA GGGATGTGGC AGCTAAGGGC 
AGCTGTGCCT GCTGGTGCCC ACTGGGCCGG AAGATCCATA TGGATGAGAC AGGCATGACC 
ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT GACGAGGTAG CCCGAGAGTT CGTGAAGCTC 
AAATCAGAGT CTCGATCCAC AGAGGAAGGG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( XM_006537795.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537858.1 Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu32860
Clone ID Related Accession (Same CDS sequence)	XM_006537795.3 , XM_006537795.4
Accession Version	XM_006537795.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537795.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTCCTT TGAAGACCCT 
GATAGTGCCG TTGATGACCG AGACAGTGAC TACCGCAGTG AGACCAGCAA TAGCGCCCCA 
CCTCCTTACC ACACGACTAC CCAGCCCAAC GCTTCTGTAC ACCAGTTCCC TGTTCCTGTG 
CGGCTGCCAC AGCAGCTCTT TCTTCAAGGC AGTTCCCATG ACTCTTGCAA TGACTCTATG 
CAGAGTTACG ACCTTGATTA TCCAGAGCGT CGGGCCCTCA GCCCTACCAG CAGTAGTAGG 
TATGGCTCCT CCTGTAATGT GAGTCAAGGA AGCTCTCTGC TGAGTGAGCT GGACCAGTAT 
CATGAGCAAG ATGATGATGG TCGGGAGAGG GACTCCATTC ATTCTAGTCA TAGCTATGGC 
AGCCTCTCCA AAGATGGCCA GGCTGGTTTG GGAGAACAAG AGAAAGCCTT GGAAGTGACA 
TGTGAATCAG AAAAAGAGAA AACAGGTGAA TCCAAGGAGA TGAGAGATGA TGCTACAATC 
CACCCTCCTT CAGATCTGGT GCTGCATAAG GACCATGTCC TAGGACCCCA GGAGAGTCTT 
CCTGAGGAGA CCGCATCATC GCCATTTACC CAGGCCAGAG CACACTGGTT CCGAGCGGTT 
ACCAAGGTCC GACTCCAACT GCAGGAGATT TCAGATGATG GTGACCCCTC CTTGCCTCAG 
TGGCTCCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGA 
AGGAAGAAGC CACTGCCACT TGTCAGTGAT CTGTCACTGG TCCAGTCACG GAAAGCTGGG 
ATTACATCTG CTATGGCTAC ACGTACCTCT CTCAAGGATG AAGAACTGAA ATCTCATGTG 
TATAAGAAAA CTCTGCAGGC CTTAATCTAC CCCATCTCGT GTACCACGCC CCACAACTTT 
GAGGTCTGGT CTGCCACTAC ACCTACCTAT TGCTATGAGT GTGAAGGCTT GCTCTGGGGC 
CTTGCCCGGC AAGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG 
GACCTGCTCA ATGCTGACTG CCTACAGCGG GCTGCTGAAA AGAGCTCTAA GCATGGAGCT 
GAGGACCGAA CTCAGAACAT TATCATGGCC ATGAAGGACC GTATGAAGAT CCGAGAACGG 
AATAAACCAG AGATCTTTGA GGTTATCCGG GATGTGTTCA CAGTGAGCAA AGTTGCGCAT 
GTGCAGCAGA TGAAAACAGT AAAGCAGAGT GTCCTGGATG GCACCTCCAA GTGGTCAGCC 
AAAATTACCA TCACGGTGGT TTGTGCCCAG GGCCTACAAG CCAAAGACAA GACAGGATCC 
AGTGACCCTT ATGTGACTGT GCAAGTTGGG AAAACCAAGA AGCGCACCAA GACCATTTTT 
GGAAATTTGA ATCCTGTTTG GGAAGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAT 
CGAATTAAGG TACGCGTGTG GGATGAGGAT GATGACATCA AGTCCAGAGT AAAGCAGCGG 
CTAAAGCGAG AGTCAGATGA TTTCCTTGGC CAAACTATCA TTGAAGTTCG AACGCTGAGT 
GGAGAGATGG ATGTCTGGTA CAATCTGGAG AAGAGAACAG ACAAGTCAGC TGTCTCAGGG 
GCTATCCGAC TTCAAATCAG TGTGGAGATC AAGGGGGAGG AGAAGGTAGC CCCATACCAT 
GTACAATATA CATGTCTCCA TGAGAACCTT TTCCATTACC TCACAGACAT TCAGGGCAGT 
GGAGGAGTCT GGATCCCAGA GGCTCGGGGG GACGATGCAT GGAAGGTGTA CTTTGATGAG 
ACAGCCCAAG AAATTGTGGA TGAATTCGCT ATGCGCTATG GCATCGAGTC CATTTATCAG 
GCCATGACGC ACTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT ACCAGCCGTG 
ATGAGCACCT TACTGGCCAA CATCAATGCC TATTATGCTC ACACAACTGC CTCCACTAAC 
GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGTA AAGAAAGATT TGTAAAACTG 
CTGGACCAGC TACACAACTC ACTGAGGATA GACCTCTCTA CATACAGGAA TAACTTTCCT 
GCTGGGAGCC CTGAGCGGCT TCAGGATTTA AAGTCTACAG TGGATTTGCT GACCAGCATT 
ACTTTCTTCA GGATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTGGTA 
AAGGACTGTG TGAAAGCCTG CTTGAACTCT ACATATGAAT ACATCTTCAA CAACTGCCAT 
GACCTCTACA GTCACCAGTA TCAGCTGCAG GAACAACCTC TAGAGGAACC AGGGCCCAGC 
ATTCGGAACT TGGATTTCTG GCCCAAACTT ATCACACTCA TTGTTTCGAT CATAGAGGAG 
GATAAGAATT CCTACACACC TGTTCTGAGC CAGTTTCCTC AGGAGTTGAA CGTAGGAAAA 
GTCAGTGCAG AAGTGATGTG GCATCTGTTT GCTCAAGACA TGAAATATGC ACTGGAGGAA 
CACGAGAAAG ACCGGCTGTG TAAGAGTGCT GACTACATGA ACCTGCACTT CAAGGTGAAG 
TGGCTCCACA ATGAATATGT GCGCGATCTG CCTGCCCTCC AGGGGCAAGT GCCTGAGTAC 
CCAGCGTGGT TTGAGCAGTT TGTGCTACAG TGGCTAGATG AGAATGAAGA TGTGTCACTG 
GAATTCCTTC GTGGGGCCTT GGAACGAGAT AAGAAGGATG GGTTCCAGCA GACATCAGAG 
CATGCCTTGT TTTCCTGTTC TGTGGTGGAT GTCTTTACAC AACTCAACCA GAGCTTTGAG 
ATCATCCGGA AGTTGGAATG TCCAGACCCC AACATCCTTG CCCACTACAT GAGAAGGTTT 
GCTAAGACCA TTGGGAAAGT GCTGATGCAG TATGCAGACA TCTTATCAAA GAACTTCCCA 
GCTTACTGCA CAAAGGAGAG ACTGCCCTGT ATCCTGATGA ACAACATGCA GCAACTGAGG 
GTCCAGCTGG AGAAAATGTT TGAGGCCATG GGAGGCAAAG AGCTGGACTC CGAAGCTGCA 
GACAGTCTGA AGGAGCTGCA GGTGAAACTG AATACAGTTC TGGATGAGCT TAGCATGGTG 
TTTGGAAACA GCTTCCAGGT GCGGATTGAT GAATGTGTTC GACAAATGGC TGACATCCTG 
GGCCAAGTTC GGGGCACAGG GAATGCATCC CCTAACGCCA GGGCCTCGGT GGCTCAGGAT 
GCAGATAGTG TGCTGCGACC TCTCATGGAC TTCCTGGATG GCAACCTCAC GCTGTTTGCC 
ACTGTGTGTG AGAAGACAGT TCTGAAGCGG GTCCTGAAGG AGCTCTGGCG GGTGGTGATG 
AACACCATGG AGCGGGTTAT TGTCCTGCCC CCACTCACTG ACCAGACGGG CACCCAGCTG 
ATCTTAACTG CTGCCAAGGA GCTGAGCCAG CTTTCCAAAC TCAAGGACCA CATGGTACGG 
GAGGAAACAC GGAATCTCAC TCCAAAGCAG TGTGCTGTCC TCGACTTGGC CTTGGACACC 
ATCAAGCAAT ACTTCCATGC AGGAGGCAAT GGTCTGAAGA AAACCTTCCT GGAAAAGAGC 
CCAGACCTGC AGTCTCTGCG TTATGCCCTC TCTCTGTATA CACAGACTAC AGACACTCTC 
ATCAAGACAT TTGTGCGCTC ACAGACTGCC CAGGTACATG ATGGGAAAGG TATTAGGTTT 
ACTGCTAACG AGGACATTCG GCCAGAAAAG GGGGCTGGTG TGGATGACCC CGTGGGAGAA 
GTCTCTATTC AGGTGGACTT ATTTACACAT CCTGGCACTG GGGAGCATAA GGTCACAGTG 
AAAGTGGTAG CAGCCAATGA CCTCAAGTGG CAGACAGCGG GTATGTTCCG GCCTTTTGTG 
GAAGTGACCA TGGTTGGCCC ACACCAAAGT GATAAGAAGA GGAAGTTCAC AACCAAGTCT 
AAAAGTAACA ACTGGACTCC CAAGTACAAC GAGACGTTTC ACTTCCTCCT GGGAAATGAA 
GAGGGTCCAG AGGCCTATGA ATTGCAGATC TGTGTGAAGG ATTACTGCTT TGCCCGGGAG 
GATCGTGTGA TAGGACTGGC AGTGATGCCC CTGAGGGATG TGGCAGCTAA GGGCAGCTGT 
GCCTGCTGGT GCCCACTGGG CCGGAAGATC CATATGGATG AGACAGGCAT GACCATTCTC 
CGGATTCTGT CTCAGAGGAG CAATGACGAG GTAGCCCGAG AGTTCGTGAA GCTCAAATCA 
GAGTCTCGAT CCACAGAGGA AGGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537858.1
CDS	336..5165
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS

Target ORF information:

RefSeq Version	XM_006537795.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog B (C. elegans) (Unc13b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537795.3

ORF Insert Sequence:

ATGTCGCTGC TCTGTGTGCG TGTTAAAAGA GCCAAATTCC AGGGTTCACC AGATAAATTT 
AACACATATG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA GGATTTCATG TTTGAGATCA GTCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA GGGACTGATA TGGGACACAA TGGTGGGCAC CGTGTGGATT 
GCACTGAAGA CAATCCGTCA GTCGGATGAG GAAGGGCCCG GGGAGTGGTC TACACTGGAG 
GCAGAGACGT TAATGAAAGA TGATGAGATC TGTGGAACTA AAAATCCAAC TCCTCATAAA 
ATCTTGCTTG ATACAAGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAGCA AATCAATGCC TTGGCAGATG ATAATGAGTA TTCTAGTCAA 
GAAGAAAGCC AGAGGAAGCC ATTACCCACT GCTGCCGCCC AGTGTTCCTT TGAAGACCCT 
GATAGTGCCG TTGATGACCG AGACAGTGAC TACCGCAGTG AGACCAGCAA TAGCGCCCCA 
CCTCCTTACC ACACGACTAC CCAGCCCAAC GCTTCTGTAC ACCAGTTCCC TGTTCCTGTG 
CGGCTGCCAC AGCAGCTCTT TCTTCAAGGC AGTTCCCATG ACTCTTGCAA TGACTCTATG 
CAGAGTTACG ACCTTGATTA TCCAGAGCGT CGGGCCCTCA GCCCTACCAG CAGTAGTAGG 
TATGGCTCCT CCTGTAATGT GAGTCAAGGA AGCTCTCTGC TGAGTGAGCT GGACCAGTAT 
CATGAGCAAG ATGATGATGG TCGGGAGAGG GACTCCATTC ATTCTAGTCA TAGCTATGGC 
AGCCTCTCCA AAGATGGCCA GGCTGGTTTG GGAGAACAAG AGAAAGCCTT GGAAGTGACA 
TGTGAATCAG AAAAAGAGAA AACAGGTGAA TCCAAGGAGA TGAGAGATGA TGCTACAATC 
CACCCTCCTT CAGATCTGGT GCTGCATAAG GACCATGTCC TAGGACCCCA GGAGAGTCTT 
CCTGAGGAGA CCGCATCATC GCCATTTACC CAGGCCAGAG CACACTGGTT CCGAGCGGTT 
ACCAAGGTCC GACTCCAACT GCAGGAGATT TCAGATGATG GTGACCCCTC CTTGCCTCAG 
TGGCTCCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC AGATCTACGA 
AGGAAGAAGC CACTGCCACT TGTCAGTGAT CTGTCACTGG TCCAGTCACG GAAAGCTGGG 
ATTACATCTG CTATGGCTAC ACGTACCTCT CTCAAGGATG AAGAACTGAA ATCTCATGTG 
TATAAGAAAA CTCTGCAGGC CTTAATCTAC CCCATCTCGT GTACCACGCC CCACAACTTT 
GAGGTCTGGT CTGCCACTAC ACCTACCTAT TGCTATGAGT GTGAAGGCTT GCTCTGGGGC 
CTTGCCCGGC AAGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG 
GACCTGCTCA ATGCTGACTG CCTACAGCGG GCTGCTGAAA AGAGCTCTAA GCATGGAGCT 
GAGGACCGAA CTCAGAACAT TATCATGGCC ATGAAGGACC GTATGAAGAT CCGAGAACGG 
AATAAACCAG AGATCTTTGA GGTTATCCGG GATGTGTTCA CAGTGAGCAA AGTTGCGCAT 
GTGCAGCAGA TGAAAACAGT AAAGCAGAGT GTCCTGGATG GCACCTCCAA GTGGTCAGCC 
AAAATTACCA TCACGGTGGT TTGTGCCCAG GGCCTACAAG CCAAAGACAA GACAGGATCC 
AGTGACCCTT ATGTGACTGT GCAAGTTGGG AAAACCAAGA AGCGCACCAA GACCATTTTT 
GGAAATTTGA ATCCTGTTTG GGAAGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAT 
CGAATTAAGG TACGCGTGTG GGATGAGGAT GATGACATCA AGTCCAGAGT AAAGCAGCGG 
CTAAAGCGAG AGTCAGATGA TTTCCTTGGC CAAACTATCA TTGAAGTTCG AACGCTGAGT 
GGAGAGATGG ATGTCTGGTA CAATCTGGAG AAGAGAACAG ACAAGTCAGC TGTCTCAGGG 
GCTATCCGAC TTCAAATCAG TGTGGAGATC AAGGGGGAGG AGAAGGTAGC CCCATACCAT 
GTACAATATA CATGTCTCCA TGAGAACCTT TTCCATTACC TCACAGACAT TCAGGGCAGT 
GGAGGAGTCT GGATCCCAGA GGCTCGGGGG GACGATGCAT GGAAGGTGTA CTTTGATGAG 
ACAGCCCAAG AAATTGTGGA TGAATTCGCT ATGCGCTATG GCATCGAGTC CATTTATCAG 
GCCATGACGC ACTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT ACCAGCCGTG 
ATGAGCACCT TACTGGCCAA CATCAATGCC TATTATGCTC ACACAACTGC CTCCACTAAC 
GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGTA AAGAAAGATT TGTAAAACTG 
CTGGACCAGC TACACAACTC ACTGAGGATA GACCTCTCTA CATACAGGAA TAACTTTCCT 
GCTGGGAGCC CTGAGCGGCT TCAGGATTTA AAGTCTACAG TGGATTTGCT GACCAGCATT 
ACTTTCTTCA GGATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTGGTA 
AAGGACTGTG TGAAAGCCTG CTTGAACTCT ACATATGAAT ACATCTTCAA CAACTGCCAT 
GACCTCTACA GTCACCAGTA TCAGCTGCAG GAACAACCTC TAGAGGAACC AGGGCCCAGC 
ATTCGGAACT TGGATTTCTG GCCCAAACTT ATCACACTCA TTGTTTCGAT CATAGAGGAG 
GATAAGAATT CCTACACACC TGTTCTGAGC CAGTTTCCTC AGGAGTTGAA CGTAGGAAAA 
GTCAGTGCAG AAGTGATGTG GCATCTGTTT GCTCAAGACA TGAAATATGC ACTGGAGGAA 
CACGAGAAAG ACCGGCTGTG TAAGAGTGCT GACTACATGA ACCTGCACTT CAAGGTGAAG 
TGGCTCCACA ATGAATATGT GCGCGATCTG CCTGCCCTCC AGGGGCAAGT GCCTGAGTAC 
CCAGCGTGGT TTGAGCAGTT TGTGCTACAG TGGCTAGATG AGAATGAAGA TGTGTCACTG 
GAATTCCTTC GTGGGGCCTT GGAACGAGAT AAGAAGGATG GGTTCCAGCA GACATCAGAG 
CATGCCTTGT TTTCCTGTTC TGTGGTGGAT GTCTTTACAC AACTCAACCA GAGCTTTGAG 
ATCATCCGGA AGTTGGAATG TCCAGACCCC AACATCCTTG CCCACTACAT GAGAAGGTTT 
GCTAAGACCA TTGGGAAAGT GCTGATGCAG TATGCAGACA TCTTATCAAA GAACTTCCCA 
GCTTACTGCA CAAAGGAGAG ACTGCCCTGT ATCCTGATGA ACAACATGCA GCAACTGAGG 
GTCCAGCTGG AGAAAATGTT TGAGGCCATG GGAGGCAAAG AGCTGGACTC CGAAGCTGCA 
GACAGTCTGA AGGAGCTGCA GGTGAAACTG AATACAGTTC TGGATGAGCT TAGCATGGTG 
TTTGGAAACA GCTTCCAGGT GCGGATTGAT GAATGTGTTC GACAAATGGC TGACATCCTG 
GGCCAAGTTC GGGGCACAGG GAATGCATCC CCTAACGCCA GGGCCTCGGT GGCTCAGGAT 
GCAGATAGTG TGCTGCGACC TCTCATGGAC TTCCTGGATG GCAACCTCAC GCTGTTTGCC 
ACTGTGTGTG AGAAGACAGT TCTGAAGCGG GTCCTGAAGG AGCTCTGGCG GGTGGTGATG 
AACACCATGG AGCGGGTTAT TGTCCTGCCC CCACTCACTG ACCAGACGGG CACCCAGCTG 
ATCTTAACTG CTGCCAAGGA GCTGAGCCAG CTTTCCAAAC TCAAGGACCA CATGGTACGG 
GAGGAAACAC GGAATCTCAC TCCAAAGCAG TGTGCTGTCC TCGACTTGGC CTTGGACACC 
ATCAAGCAAT ACTTCCATGC AGGAGGCAAT GGTCTGAAGA AAACCTTCCT GGAAAAGAGC 
CCAGACCTGC AGTCTCTGCG TTATGCCCTC TCTCTGTATA CACAGACTAC AGACACTCTC 
ATCAAGACAT TTGTGCGCTC ACAGACTGCC CAGGTACATG ATGGGAAAGG TATTAGGTTT 
ACTGCTAACG AGGACATTCG GCCAGAAAAG GGGGCTGGTG TGGATGACCC CGTGGGAGAA 
GTCTCTATTC AGGTGGACTT ATTTACACAT CCTGGCACTG GGGAGCATAA GGTCACAGTG 
AAAGTGGTAG CAGCCAATGA CCTCAAGTGG CAGACAGCGG GTATGTTCCG GCCTTTTGTG 
GAAGTGACCA TGGTTGGCCC ACACCAAAGT GATAAGAAGA GGAAGTTCAC AACCAAGTCT 
AAAAGTAACA ACTGGACTCC CAAGTACAAC GAGACGTTTC ACTTCCTCCT GGGAAATGAA 
GAGGGTCCAG AGGCCTATGA ATTGCAGATC TGTGTGAAGG ATTACTGCTT TGCCCGGGAG 
GATCGTGTGA TAGGACTGGC AGTGATGCCC CTGAGGGATG TGGCAGCTAA GGGCAGCTGT 
GCCTGCTGGT GCCCACTGGG CCGGAAGATC CATATGGATG AGACAGGCAT GACCATTCTC 
CGGATTCTGT CTCAGAGGAG CAATGACGAG GTAGCCCGAG AGTTCGTGAA GCTCAAATCA 
GAGTCTCGAT CCACAGAGGA AGGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Munc13 proteins control regulated exocytosis in mast cells.
The Journal of biological chemistry293(1)345-358(2018 Jan)
Rodarte EM,Ramos MA,Davalos AJ,Moreira DC,Moreno DS,Cardenas EI,Rodarte AI,Petrova Y,Molina S,Rendon LE,Sanchez E,Breaux K,Tortoriello A,Manllo J,Gonzalez EA,Tuvim MJ,Dickey BF,Burns AR,Heidelberger R,Adachi R

Doc2B acts as a calcium sensor for vesicle priming requiring synaptotagmin-1, Munc13-2 and SNAREs.
eLife6(2017 Dec)
Houy S,Groffen AJ,Ziomkiewicz I,Verhage M,Pinheiro PS,S?rensen JB

Phosphatidylinositol 4,5-bisphosphate optical uncaging potentiates exocytosis.
eLife6(2017 Oct)
Walter AM,M?ller R,Tawfik B,Wierda KD,Pinheiro PS,Nadler A,McCarthy AW,Ziomkiewicz I,Kruse M,Reither G,Rettig J,Lehmann M,Haucke V,Hille B,Schultz C,S?rensen JB

Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18.
Neuron95(3)591-607.e10(2017 Aug)
Lai Y,Choi UB,Leitz J,Rhee HJ,Lee C,Altas B,Zhao M,Pfuetzner RA,Wang AL,Brose N,Rhee J,Brunger AT

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

FAX

1-732-210-0262

Unc13b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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