March6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	March6
Entrez Gene ID	223455
Full Name	membrane-associated ring finger (C3HC4) 6
Synonyms	3830408G03,F830029L24Rik,mKIAA0597
General protein information	Preferred Names membrane-associated ring finger (C3HC4) 6 Names E3 ubiquitin-protein ligase MARCH6 MARCH-VI RING-type E3 ubiquitin transferase MARCH6 membrane-associated RING finger protein 6 membrane-associated RING-CH protein VI
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_172606.2	NP_766194.2	E3 ubiquitin-protein ligase MARCH6
XM_011245351.2	XP_011243653.1	E3 ubiquitin-protein ligase MARCH6 isoform X1
XM_011245351.3	XP_011243653.1	E3 ubiquitin-protein ligase MARCH6 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MARCH6	XP_003310676.1
Canis lupus familiaris (dog)	MARCH6	XP_005639724.1
Gallus gallus (chicken)	MARCH6	XP_419012.3
Arabidopsis thaliana (thale cress)	AT4G34100	NP_195136.3
Mus musculus (house mouse)	March6	NP_766194.2
Drosophila melanogaster (fruit fly)	CG1317	NP_647715.2
Xenopus tropicalis (tropical clawed frog)	march6	XP_002938364.2
Homo sapiens (human)	MARCH6	NP_005876.2
Bos taurus (cattle)	MARCH6	NP_001192741.1
Rattus norvegicus (Norway rat)	March6	XP_215517.5
Danio rerio (zebrafish)	march6	XP_005163506.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007204	XP_308581.3
Caenorhabditis elegans (roundworm)	marc-6	NP_492823.2
Oryza sativa (rice)	Os06g0639000	NP_001058157.1

Gene Ontology
Bibliography

Related articles in PubMed

Tagging genes with cassette-exchange sites.
Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M, Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A, Cortese R
Nucleic acids research33(4)e44(2005 Mar)

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following March6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the March6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03761	NM_172606.2 Latest version!	Mus musculus membrane associated ring-CH-type finger 6 (Marchf6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
OMu73116	XM_011245351.3 Latest version!	Mus musculus membrane-associated ring finger (C3HC4) 6 (March6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu73116	XM_011245351.2	Mus musculus membrane-associated ring finger (C3HC4) 6 (March6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

March6 ( NM_172606.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766194.2 Mus musculus membrane associated ring-CH-type finger 6 (Marchf6), mRNA.

Price & Availability↑

CloneID	OMu03761
Clone ID Related Accession (Same CDS sequence)	NM_172606.2
Accession Version	NM_172606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MARCH6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU516606.1, CF182201.1, CF540592.1, AK042980.1 and AC115735.8. On Jul 21, 2005 this sequence version replaced NM_172606.1. ##Evidence-Data-START## Transcript exon combination :: AK132461.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCG CCGAGGAAGA TATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTATC ATCCTTGTGT ATGTACTGGC AGTATTAAAT TTATTCATCA AGAATGCTTA 
GTTCAGTGGT TGAAACATAG TCGGAAAGAA TACTGTGAAT TATGCAAGCA CAGATTTGCT 
TTCACACCAA TTTATTCTCC AGATATGCCT TCACGGCTAC CAATCCAAGA CATATTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCAATA CGATACTGGT TTCATTACAC ACTTGTGGCC 
TTTGCATGGC TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCCGTGA GCTCACTACT GACACTGCCA CTAGACATGC TGTCAACGGA AAATTTGTTG 
GCGGATTGCT TGCAGGGCTG TTTTGTGGTG ACGTGCACAC TTTGTGCGTT CATCAGCCTG 
GTGTGGTTAC GAGAGCAGAT AGTCCACGGG GGAGCCCCAA TTTGGCTGGA GCATGCTGCT 
CCACCCTTCA ATGCTGCTGG ACACCACCAA AATGAGGCTC CTGTAGGAGG AAATGGTGCA 
GAAAACCCTG CTGCTGATCA GCCAGCTAAC CCAGCAGGTG AGAATGCAGT TCTGGGTGAA 
AACCCTGATG CCCAGGACGG TCAAGCAGAA GAGGAAGAGG AGGACAATGA GGAGGAAGAT 
GATGCGGGTG TAGAAGATGC TGCTGATGCC AACAATGGAG CCCAAGACGA CATGAACTGG 
AATGCTTTAG AGTGGGACCG GGCTGCAGAA GAGCTTACTT GGGAAAGAAT GCTTGGACTT 
GATGGATCAC TAGTTTTCCT GGAACACGTG TTCTGGGTGG TGTCTTTAAA TACATTGTTC 
ATTCTTGTTT TTGCATTTTG TCCTTACCAC ATCGGCCATT TCTCCCTTGT TGGCTTGGGG 
TTTGAGGAGC ATGTCCAAGC ATCTCATTTT GAAGGTCTAA TCACAACGAT TGTTGGATAT 
ATACTTTTAG CAATAACACT GATAATTTGT CATGCATTGG CAACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG CGTCTGCTAT ATTGTGGTTA AGGTCTCTTT GTTAGTTGTG 
GTAGAAATCG GAGTGTTCCC TCTCATTTGT GGCTGGTGGC TGGATATCTG TTCTCTGGAA 
ATGTTTGATG CTACTCTGAA AGATCGAGAG CTGAGCTTTC AGTCAGCTCC AGGGACCACG 
ATGTTTCTGC ACTGGTTGGT GGGAATGGTG TATGTGTTCT ACTTTGCCTC CTTCATCCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTC TTATGGTTTC TGAGGAATTT GAACGATCCA 
GATTTTAATC CAGTACAGGA AATGATTCAC TTGCCCATTT ATAGACATCT CCGAAGATTT 
ATTTTGTCAG TGATTGTCTT TGGCTCCATT GTCCTTCTGA TGCTCTGGCT TCCTATACGT 
ATAATTAAGA GTCTCTTGCC TAATTTTCTT CCTTACAATG TCATGCTCTA CAGTGATGCT 
CCGGTGAGTG AGCTGTCCCT TGAGCTGCTC CTACTGCAGG TTGTCTTGCC AGCATTGCTG 
GAACAGGGAC ACACGAGGCA GTGGCTGAAG GGGCTTGTGC GTGCATGGAC TGTTACTGCT 
GGATACTTGC TGGACCTTCA TTCCTACTTA CTGGGAGATC AGGAAGAAAA CGAGAACAGT 
GCAAATCAGC AGGTCAACAA TAACCAGCCT GCACGAAACA ATAATGCTGT TCCTGCCGGG 
GAAGGTCTGC ATGCAGCCCA CCAAGCCATA CTCCAGCAGG GAGGACCTGT TGGCTTTCAG 
CCTTACCGCC GGCCTTTAAA TTTCCCACTC AGGATATTTC TGCTGATTGT CTTCATGTGC 
ATAACATTGC TAATTGCCAG CCTCATTTGC CTTACTTTAC CAGTATTTGC TGGCCGTTGG 
TTAATGTCAT TTTGGACGGG GACTGCCAAA ATCCATGAGC TGTACACAGC TGCTTGTGGT 
CTCTATGTGT GTTGGCTAAC CATAAGGGCT GTGACGGTGC TGGTGGCCTG GATGCCCCAG 
GGACGAAGAG TGATTTTCCA GAAGGTTAAA GAGTGGTCCC TCATGATAAT GAAGACGTTG 
ATAGTTGCTG TGCTGCTGGC TGGGGTCGTC CCACTGCTCC TCGGGCTCCT GTTTGAGCTG 
GTTATTGTTG CTCCTCTGAG GGTCCCTCTT GATCAGACTC CCCTTTTCTA CCCTTGGCAG 
GACTGGGCGC TGGGAGTGCT GCATGCCAAA ATTATCGCAG CTATAACTCT TATGGGTCCT 
CAGTGGTGGT TGAAAACTGT CATTGAACAG GTTTACGCAA ATGGCATTCG TAACATCGAC 
CTTCACTATA TCATCCGTAA GCTGGCAGCC CCGGTGATCT CTGTGCTCTT ACTTTCCCTG 
TGTGTACCGT ATGTCATAGC TTCTGGCGCG GTTCCTCTAC TAGGTGTTAC TGCAGAGATG 
CAGAACTTAG TTCACCGGCG GATTTACCCA TTTTTATTGA TGGTCGTTGT GCTGATGGGA 
ATCCTGTCCT TCCAGGTCCG CCAGTTTAAG CGCCTTTATG AACACATTAA AAATGACAAG 
TACCTTGTGG GGCAGCGCCT GGTGAACTAT GAGCGCAAAT CCGGCAAGCA AGGCCCGTCT 
ACACCGCCTC CGGTGTCGTC CCAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766194.2
CDS	217..2946
Translation	MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPVGGNGAENPAADQPANPAGENAVLGENPDAQDGQAEEEEEDNEEEDDAGVEDAADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHALATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSLLPNFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQPARNNNAVPAGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVLVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHYIIRKLAAPVISVLLLSLCVPYVIASGAVPLLGVTAEMQNLVHRRIYPFLLMVVVLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGPSTPPPVSSQE

Target ORF information:

RefSeq Version	NM_172606.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane associated ring-CH-type finger 6 (Marchf6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172606.2

ORF Insert Sequence:

ATGGACACCG CCGAGGAAGA TATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTATC ATCCTTGTGT ATGTACTGGC AGTATTAAAT TTATTCATCA AGAATGCTTA 
GTTCAGTGGT TGAAACATAG TCGGAAAGAA TACTGTGAAT TATGCAAGCA CAGATTTGCT 
TTCACACCAA TTTATTCTCC AGATATGCCT TCACGGCTAC CAATCCAAGA CATATTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCAATA CGATACTGGT TTCATTACAC ACTTGTGGCC 
TTTGCATGGC TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCCGTGA GCTCACTACT GACACTGCCA CTAGACATGC TGTCAACGGA AAATTTGTTG 
GCGGATTGCT TGCAGGGCTG TTTTGTGGTG ACGTGCACAC TTTGTGCGTT CATCAGCCTG 
GTGTGGTTAC GAGAGCAGAT AGTCCACGGG GGAGCCCCAA TTTGGCTGGA GCATGCTGCT 
CCACCCTTCA ATGCTGCTGG ACACCACCAA AATGAGGCTC CTGTAGGAGG AAATGGTGCA 
GAAAACCCTG CTGCTGATCA GCCAGCTAAC CCAGCAGGTG AGAATGCAGT TCTGGGTGAA 
AACCCTGATG CCCAGGACGG TCAAGCAGAA GAGGAAGAGG AGGACAATGA GGAGGAAGAT 
GATGCGGGTG TAGAAGATGC TGCTGATGCC AACAATGGAG CCCAAGACGA CATGAACTGG 
AATGCTTTAG AGTGGGACCG GGCTGCAGAA GAGCTTACTT GGGAAAGAAT GCTTGGACTT 
GATGGATCAC TAGTTTTCCT GGAACACGTG TTCTGGGTGG TGTCTTTAAA TACATTGTTC 
ATTCTTGTTT TTGCATTTTG TCCTTACCAC ATCGGCCATT TCTCCCTTGT TGGCTTGGGG 
TTTGAGGAGC ATGTCCAAGC ATCTCATTTT GAAGGTCTAA TCACAACGAT TGTTGGATAT 
ATACTTTTAG CAATAACACT GATAATTTGT CATGCATTGG CAACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG CGTCTGCTAT ATTGTGGTTA AGGTCTCTTT GTTAGTTGTG 
GTAGAAATCG GAGTGTTCCC TCTCATTTGT GGCTGGTGGC TGGATATCTG TTCTCTGGAA 
ATGTTTGATG CTACTCTGAA AGATCGAGAG CTGAGCTTTC AGTCAGCTCC AGGGACCACG 
ATGTTTCTGC ACTGGTTGGT GGGAATGGTG TATGTGTTCT ACTTTGCCTC CTTCATCCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTC TTATGGTTTC TGAGGAATTT GAACGATCCA 
GATTTTAATC CAGTACAGGA AATGATTCAC TTGCCCATTT ATAGACATCT CCGAAGATTT 
ATTTTGTCAG TGATTGTCTT TGGCTCCATT GTCCTTCTGA TGCTCTGGCT TCCTATACGT 
ATAATTAAGA GTCTCTTGCC TAATTTTCTT CCTTACAATG TCATGCTCTA CAGTGATGCT 
CCGGTGAGTG AGCTGTCCCT TGAGCTGCTC CTACTGCAGG TTGTCTTGCC AGCATTGCTG 
GAACAGGGAC ACACGAGGCA GTGGCTGAAG GGGCTTGTGC GTGCATGGAC TGTTACTGCT 
GGATACTTGC TGGACCTTCA TTCCTACTTA CTGGGAGATC AGGAAGAAAA CGAGAACAGT 
GCAAATCAGC AGGTCAACAA TAACCAGCCT GCACGAAACA ATAATGCTGT TCCTGCCGGG 
GAAGGTCTGC ATGCAGCCCA CCAAGCCATA CTCCAGCAGG GAGGACCTGT TGGCTTTCAG 
CCTTACCGCC GGCCTTTAAA TTTCCCACTC AGGATATTTC TGCTGATTGT CTTCATGTGC 
ATAACATTGC TAATTGCCAG CCTCATTTGC CTTACTTTAC CAGTATTTGC TGGCCGTTGG 
TTAATGTCAT TTTGGACGGG GACTGCCAAA ATCCATGAGC TGTACACAGC TGCTTGTGGT 
CTCTATGTGT GTTGGCTAAC CATAAGGGCT GTGACGGTGC TGGTGGCCTG GATGCCCCAG 
GGACGAAGAG TGATTTTCCA GAAGGTTAAA GAGTGGTCCC TCATGATAAT GAAGACGTTG 
ATAGTTGCTG TGCTGCTGGC TGGGGTCGTC CCACTGCTCC TCGGGCTCCT GTTTGAGCTG 
GTTATTGTTG CTCCTCTGAG GGTCCCTCTT GATCAGACTC CCCTTTTCTA CCCTTGGCAG 
GACTGGGCGC TGGGAGTGCT GCATGCCAAA ATTATCGCAG CTATAACTCT TATGGGTCCT 
CAGTGGTGGT TGAAAACTGT CATTGAACAG GTTTACGCAA ATGGCATTCG TAACATCGAC 
CTTCACTATA TCATCCGTAA GCTGGCAGCC CCGGTGATCT CTGTGCTCTT ACTTTCCCTG 
TGTGTACCGT ATGTCATAGC TTCTGGCGCG GTTCCTCTAC TAGGTGTTAC TGCAGAGATG 
CAGAACTTAG TTCACCGGCG GATTTACCCA TTTTTATTGA TGGTCGTTGT GCTGATGGGA 
ATCCTGTCCT TCCAGGTCCG CCAGTTTAAG CGCCTTTATG AACACATTAA AAATGACAAG 
TACCTTGTGG GGCAGCGCCT GGTGAACTAT GAGCGCAAAT CCGGCAAGCA AGGCCCGTCT 
ACACCGCCTC CGGTGTCGTC CCAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

March6 ( XM_011245351.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243653.1 Mus musculus membrane-associated ring finger (C3HC4) 6 (March6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73116
Clone ID Related Accession (Same CDS sequence)	XM_011245351.2 , XM_011245351.3
Accession Version	XM_011245351.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MARCH6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCG CCGAGGAAGA TATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTATC ATCCTTGTGT ATGTACTGGC AGTATTAAAT TTATTCATCA AGAATGCTTA 
GTTCAGTGGT TGAAACATAG TCGGAAAGAA TACTGTGAAT TATGCAAGCA CAGATTTGCT 
TTCACACCAA TTTATTCTCC AGATATGCCT TCACGGCTAC CAATCCAAGA CATATTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCAATA CGATACTGGT TTCATTACAC ACTTGTGGCC 
TTTGCATGGC TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCCGTGA GCTCACTACT GACACTGCCA CTAGACATGC TGTCAACGGA AAATTTGTTG 
GCGGATTGCT TGCAGGGCTG TTTTGTGGTG ACGTGCACAC TTTGTGCGTT CATCAGCCTG 
GTGTGGTTAC GAGAGCAGAT AGTCCACGGG GGAGCCCCAA TTTGGCTGGA GCATGCTGCT 
CCACCCTTCA ATGCTGCTGG ACACCACCAA AATGAGGCTC CTGTAGGAGG AAATGGTGCA 
GAAAACCCTG CTGCTGATCA GCCAGCTAAC CCAGCAGGTG AGAATGCAGT TCTGGGTGAA 
AACCCTGATG CCCAGGACGG TCAAGCAGAA GAGGAAGAGG AGGACAATGA GGAGGAAGAT 
GATGCGGGTG TAGAAGATGC TGCTGATGCC AACAATGGAG CCCAAGACGA CATGAACTGG 
AATGCTTTAG AGTGGGACCG GGCTGCAGAA GAGCTTACTT GGGAAAGAAT GCTTGGACTT 
GATGGATCAC TAGTTTTCCT GGAACACGTG TTCTGGGTGG TGTCTTTAAA TACATTGTTC 
ATTCTTGTTT TTGCATTTTG TCCTTACCAC ATCGGCCATT TCTCCCTTGT TGGCTTGGGG 
TTTGAGGAGC ATGTCCAAGC ATCTCATTTT GAAGGTCTAA TCACAACGAT TGTTGGATAT 
ATACTTTTAG CAATAACACT GATAATTTGT CATGCATTGG CAACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG CGTCTGCTAT ATTGTGGTTA AGGTCTCTTT GTTAGTTGTG 
GTAGAAATCG GAGTGTTCCC TCTCATTTGT GGCTGGTGGC TGGATATCTG TTCTCTGGAA 
ATGTTTGATG CTACTCTGAA AGATCGAGAG CTGAGCTTTC AGTCAGCTCC AGGGACCACG 
ATGTTTCTGC ACTGGTTGGT GGGAATGGTG TATGTGTTCT ACTTTGCCTC CTTCATCCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTC TTATGGTTTC TGAGGAATTT GAACGATCCA 
GATTTTAATC CAGTACAGGA AATGATTCAC TTGCCCATTT ATAGACATCT CCGAAGATTT 
ATTTTGTCAG TGATTGTCTT TGGCTCCATT GTCCTTCTGA TGCTCTGGCT TCCTATACGT 
ATAATTAAGA GTCTCTTGCC TAATTTTCTT CCTTACAATG TCATGCTCTA CAGTGATGCT 
CCGGTGAGTG AGCTGTCCCT TGAGCTGCTC CTACTGCAGG TTGTCTTGCC AGCATTGCTG 
GAACAGGGAC ACACGAGGCA GTGGCTGAAG GGGCTTGTGC GTGCATGGAC TGTTACTGCT 
GGATACTTGC TGGACCTTCA TTCCTACTTA CTGGGAGATC AGGAAGAAAA CGAGAACAGT 
GCAAATCAGC AGGTCAACAA TAACCAGCCT GCACGAAACA ATAATGCTGT TCCTGCCGGG 
GAAGGTCTGC ATGCAGCCCA CCAAGCCATA CTCCAGCAGG GAGGACCTGT TGGCTTTCAG 
CCTTACCGCC GGCCTTTAAA TTTCCCACTC AGGATATTTC TGCTGATTGT CTTCATGTGC 
ATAACATTGC TAATTGCCAG CCTCATTTGC CTTACTTTAC CAGCCTTTCT ATTTTCAGTA 
TTTGCTGGCC GTTGGTTAAT GTCATTTTGG ACGGGGACTG CCAAAATCCA TGAGCTGTAC 
ACAGCTGCTT GTGGTCTCTA TGTGTGTTGG CTAACCATAA GGGCTGTGAC GGTGCTGGTG 
GCCTGGATGC CCCAGGGACG AAGAGTGATT TTCCAGAAGG TTAAAGAGTG GTCCCTCATG 
ATAATGAAGA CGTTGATAGT TGCTGTGCTG CTGGCTGGGG TCGTCCCACT GCTCCTCGGG 
CTCCTGTTTG AGCTGGTTAT TGTTGCTCCT CTGAGGGTCC CTCTTGATCA GACTCCCCTT 
TTCTACCCTT GGCAGGACTG GGCGCTGGGA GTGCTGCATG CCAAAATTAT CGCAGCTATA 
ACTCTTATGG GTCCTCAGTG GTGGTTGAAA ACTGTCATTG AACAGGTTTA CGCAAATGGC 
ATTCGTAACA TCGACCTTCA CTATATCATC CGTAAGCTGG CAGCCCCGGT GATCTCTGTG 
CTCTTACTTT CCCTGTGTGT ACCGTATGTC ATAGCTTCTG GCGCGGTTCC TCTACTAGGT 
GTTACTGCAG AGATGCAGAA CTTAGTTCAC CGGCGGATTT ACCCATTTTT ATTGATGGTC 
GTTGTGCTGA TGGGAATCCT GTCCTTCCAG GTCCGCCAGT TTAAGCGCCT TTATGAACAC 
ATTAAAAATG ACAAGTACCT TGTGGGGCAG CGCCTGGTGA ACTATGAGCG CAAATCCGGC 
AAGCAAGGCC CGTCTACACC GCCTCCGGTG TCGTCCCAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243653.1
CDS	228..2972
Translation	MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPVGGNGAENPAADQPANPAGENAVLGENPDAQDGQAEEEEEDNEEEDDAGVEDAADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHALATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSLLPNFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQPARNNNAVPAGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPAFLFSVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVLVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHYIIRKLAAPVISVLLLSLCVPYVIASGAVPLLGVTAEMQNLVHRRIYPFLLMVVVLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGPSTPPPVSSQE

Target ORF information:

RefSeq Version	XM_011245351.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane-associated ring finger (C3HC4) 6 (March6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245351.2

ORF Insert Sequence:

ATGGACACCG CCGAGGAAGA TATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTATC ATCCTTGTGT ATGTACTGGC AGTATTAAAT TTATTCATCA AGAATGCTTA 
GTTCAGTGGT TGAAACATAG TCGGAAAGAA TACTGTGAAT TATGCAAGCA CAGATTTGCT 
TTCACACCAA TTTATTCTCC AGATATGCCT TCACGGCTAC CAATCCAAGA CATATTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCAATA CGATACTGGT TTCATTACAC ACTTGTGGCC 
TTTGCATGGC TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCCGTGA GCTCACTACT GACACTGCCA CTAGACATGC TGTCAACGGA AAATTTGTTG 
GCGGATTGCT TGCAGGGCTG TTTTGTGGTG ACGTGCACAC TTTGTGCGTT CATCAGCCTG 
GTGTGGTTAC GAGAGCAGAT AGTCCACGGG GGAGCCCCAA TTTGGCTGGA GCATGCTGCT 
CCACCCTTCA ATGCTGCTGG ACACCACCAA AATGAGGCTC CTGTAGGAGG AAATGGTGCA 
GAAAACCCTG CTGCTGATCA GCCAGCTAAC CCAGCAGGTG AGAATGCAGT TCTGGGTGAA 
AACCCTGATG CCCAGGACGG TCAAGCAGAA GAGGAAGAGG AGGACAATGA GGAGGAAGAT 
GATGCGGGTG TAGAAGATGC TGCTGATGCC AACAATGGAG CCCAAGACGA CATGAACTGG 
AATGCTTTAG AGTGGGACCG GGCTGCAGAA GAGCTTACTT GGGAAAGAAT GCTTGGACTT 
GATGGATCAC TAGTTTTCCT GGAACACGTG TTCTGGGTGG TGTCTTTAAA TACATTGTTC 
ATTCTTGTTT TTGCATTTTG TCCTTACCAC ATCGGCCATT TCTCCCTTGT TGGCTTGGGG 
TTTGAGGAGC ATGTCCAAGC ATCTCATTTT GAAGGTCTAA TCACAACGAT TGTTGGATAT 
ATACTTTTAG CAATAACACT GATAATTTGT CATGCATTGG CAACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG CGTCTGCTAT ATTGTGGTTA AGGTCTCTTT GTTAGTTGTG 
GTAGAAATCG GAGTGTTCCC TCTCATTTGT GGCTGGTGGC TGGATATCTG TTCTCTGGAA 
ATGTTTGATG CTACTCTGAA AGATCGAGAG CTGAGCTTTC AGTCAGCTCC AGGGACCACG 
ATGTTTCTGC ACTGGTTGGT GGGAATGGTG TATGTGTTCT ACTTTGCCTC CTTCATCCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTC TTATGGTTTC TGAGGAATTT GAACGATCCA 
GATTTTAATC CAGTACAGGA AATGATTCAC TTGCCCATTT ATAGACATCT CCGAAGATTT 
ATTTTGTCAG TGATTGTCTT TGGCTCCATT GTCCTTCTGA TGCTCTGGCT TCCTATACGT 
ATAATTAAGA GTCTCTTGCC TAATTTTCTT CCTTACAATG TCATGCTCTA CAGTGATGCT 
CCGGTGAGTG AGCTGTCCCT TGAGCTGCTC CTACTGCAGG TTGTCTTGCC AGCATTGCTG 
GAACAGGGAC ACACGAGGCA GTGGCTGAAG GGGCTTGTGC GTGCATGGAC TGTTACTGCT 
GGATACTTGC TGGACCTTCA TTCCTACTTA CTGGGAGATC AGGAAGAAAA CGAGAACAGT 
GCAAATCAGC AGGTCAACAA TAACCAGCCT GCACGAAACA ATAATGCTGT TCCTGCCGGG 
GAAGGTCTGC ATGCAGCCCA CCAAGCCATA CTCCAGCAGG GAGGACCTGT TGGCTTTCAG 
CCTTACCGCC GGCCTTTAAA TTTCCCACTC AGGATATTTC TGCTGATTGT CTTCATGTGC 
ATAACATTGC TAATTGCCAG CCTCATTTGC CTTACTTTAC CAGCCTTTCT ATTTTCAGTA 
TTTGCTGGCC GTTGGTTAAT GTCATTTTGG ACGGGGACTG CCAAAATCCA TGAGCTGTAC 
ACAGCTGCTT GTGGTCTCTA TGTGTGTTGG CTAACCATAA GGGCTGTGAC GGTGCTGGTG 
GCCTGGATGC CCCAGGGACG AAGAGTGATT TTCCAGAAGG TTAAAGAGTG GTCCCTCATG 
ATAATGAAGA CGTTGATAGT TGCTGTGCTG CTGGCTGGGG TCGTCCCACT GCTCCTCGGG 
CTCCTGTTTG AGCTGGTTAT TGTTGCTCCT CTGAGGGTCC CTCTTGATCA GACTCCCCTT 
TTCTACCCTT GGCAGGACTG GGCGCTGGGA GTGCTGCATG CCAAAATTAT CGCAGCTATA 
ACTCTTATGG GTCCTCAGTG GTGGTTGAAA ACTGTCATTG AACAGGTTTA CGCAAATGGC 
ATTCGTAACA TCGACCTTCA CTATATCATC CGTAAGCTGG CAGCCCCGGT GATCTCTGTG 
CTCTTACTTT CCCTGTGTGT ACCGTATGTC ATAGCTTCTG GCGCGGTTCC TCTACTAGGT 
GTTACTGCAG AGATGCAGAA CTTAGTTCAC CGGCGGATTT ACCCATTTTT ATTGATGGTC 
GTTGTGCTGA TGGGAATCCT GTCCTTCCAG GTCCGCCAGT TTAAGCGCCT TTATGAACAC 
ATTAAAAATG ACAAGTACCT TGTGGGGCAG CGCCTGGTGA ACTATGAGCG CAAATCCGGC 
AAGCAAGGCC CGTCTACACC GCCTCCGGTG TCGTCCCAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

March6 ( XM_011245351.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243653.1 Mus musculus membrane-associated ring finger (C3HC4) 6 (March6), transcript variant X1, mRNA.

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CloneID	OMu73116
Clone ID Related Accession (Same CDS sequence)	XM_011245351.2 , XM_011245351.3
Accession Version	XM_011245351.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MARCH6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245351.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCG CCGAGGAAGA TATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTATC ATCCTTGTGT ATGTACTGGC AGTATTAAAT TTATTCATCA AGAATGCTTA 
GTTCAGTGGT TGAAACATAG TCGGAAAGAA TACTGTGAAT TATGCAAGCA CAGATTTGCT 
TTCACACCAA TTTATTCTCC AGATATGCCT TCACGGCTAC CAATCCAAGA CATATTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCAATA CGATACTGGT TTCATTACAC ACTTGTGGCC 
TTTGCATGGC TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCCGTGA GCTCACTACT GACACTGCCA CTAGACATGC TGTCAACGGA AAATTTGTTG 
GCGGATTGCT TGCAGGGCTG TTTTGTGGTG ACGTGCACAC TTTGTGCGTT CATCAGCCTG 
GTGTGGTTAC GAGAGCAGAT AGTCCACGGG GGAGCCCCAA TTTGGCTGGA GCATGCTGCT 
CCACCCTTCA ATGCTGCTGG ACACCACCAA AATGAGGCTC CTGTAGGAGG AAATGGTGCA 
GAAAACCCTG CTGCTGATCA GCCAGCTAAC CCAGCAGGTG AGAATGCAGT TCTGGGTGAA 
AACCCTGATG CCCAGGACGG TCAAGCAGAA GAGGAAGAGG AGGACAATGA GGAGGAAGAT 
GATGCGGGTG TAGAAGATGC TGCTGATGCC AACAATGGAG CCCAAGACGA CATGAACTGG 
AATGCTTTAG AGTGGGACCG GGCTGCAGAA GAGCTTACTT GGGAAAGAAT GCTTGGACTT 
GATGGATCAC TAGTTTTCCT GGAACACGTG TTCTGGGTGG TGTCTTTAAA TACATTGTTC 
ATTCTTGTTT TTGCATTTTG TCCTTACCAC ATCGGCCATT TCTCCCTTGT TGGCTTGGGG 
TTTGAGGAGC ATGTCCAAGC ATCTCATTTT GAAGGTCTAA TCACAACGAT TGTTGGATAT 
ATACTTTTAG CAATAACACT GATAATTTGT CATGCATTGG CAACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG CGTCTGCTAT ATTGTGGTTA AGGTCTCTTT GTTAGTTGTG 
GTAGAAATCG GAGTGTTCCC TCTCATTTGT GGCTGGTGGC TGGATATCTG TTCTCTGGAA 
ATGTTTGATG CTACTCTGAA AGATCGAGAG CTGAGCTTTC AGTCAGCTCC AGGGACCACG 
ATGTTTCTGC ACTGGTTGGT GGGAATGGTG TATGTGTTCT ACTTTGCCTC CTTCATCCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTC TTATGGTTTC TGAGGAATTT GAACGATCCA 
GATTTTAATC CAGTACAGGA AATGATTCAC TTGCCCATTT ATAGACATCT CCGAAGATTT 
ATTTTGTCAG TGATTGTCTT TGGCTCCATT GTCCTTCTGA TGCTCTGGCT TCCTATACGT 
ATAATTAAGA GTCTCTTGCC TAATTTTCTT CCTTACAATG TCATGCTCTA CAGTGATGCT 
CCGGTGAGTG AGCTGTCCCT TGAGCTGCTC CTACTGCAGG TTGTCTTGCC AGCATTGCTG 
GAACAGGGAC ACACGAGGCA GTGGCTGAAG GGGCTTGTGC GTGCATGGAC TGTTACTGCT 
GGATACTTGC TGGACCTTCA TTCCTACTTA CTGGGAGATC AGGAAGAAAA CGAGAACAGT 
GCAAATCAGC AGGTCAACAA TAACCAGCCT GCACGAAACA ATAATGCTGT TCCTGCCGGG 
GAAGGTCTGC ATGCAGCCCA CCAAGCCATA CTCCAGCAGG GAGGACCTGT TGGCTTTCAG 
CCTTACCGCC GGCCTTTAAA TTTCCCACTC AGGATATTTC TGCTGATTGT CTTCATGTGC 
ATAACATTGC TAATTGCCAG CCTCATTTGC CTTACTTTAC CAGCCTTTCT ATTTTCAGTA 
TTTGCTGGCC GTTGGTTAAT GTCATTTTGG ACGGGGACTG CCAAAATCCA TGAGCTGTAC 
ACAGCTGCTT GTGGTCTCTA TGTGTGTTGG CTAACCATAA GGGCTGTGAC GGTGCTGGTG 
GCCTGGATGC CCCAGGGACG AAGAGTGATT TTCCAGAAGG TTAAAGAGTG GTCCCTCATG 
ATAATGAAGA CGTTGATAGT TGCTGTGCTG CTGGCTGGGG TCGTCCCACT GCTCCTCGGG 
CTCCTGTTTG AGCTGGTTAT TGTTGCTCCT CTGAGGGTCC CTCTTGATCA GACTCCCCTT 
TTCTACCCTT GGCAGGACTG GGCGCTGGGA GTGCTGCATG CCAAAATTAT CGCAGCTATA 
ACTCTTATGG GTCCTCAGTG GTGGTTGAAA ACTGTCATTG AACAGGTTTA CGCAAATGGC 
ATTCGTAACA TCGACCTTCA CTATATCATC CGTAAGCTGG CAGCCCCGGT GATCTCTGTG 
CTCTTACTTT CCCTGTGTGT ACCGTATGTC ATAGCTTCTG GCGCGGTTCC TCTACTAGGT 
GTTACTGCAG AGATGCAGAA CTTAGTTCAC CGGCGGATTT ACCCATTTTT ATTGATGGTC 
GTTGTGCTGA TGGGAATCCT GTCCTTCCAG GTCCGCCAGT TTAAGCGCCT TTATGAACAC 
ATTAAAAATG ACAAGTACCT TGTGGGGCAG CGCCTGGTGA ACTATGAGCG CAAATCCGGC 
AAGCAAGGCC CGTCTACACC GCCTCCGGTG TCGTCCCAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243653.1
CDS	262..3006
Translation	MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPVGGNGAENPAADQPANPAGENAVLGENPDAQDGQAEEEEEDNEEEDDAGVEDAADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHALATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSLLPNFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQPARNNNAVPAGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPAFLFSVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVLVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHYIIRKLAAPVISVLLLSLCVPYVIASGAVPLLGVTAEMQNLVHRRIYPFLLMVVVLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGPSTPPPVSSQE

Target ORF information:

RefSeq Version	XM_011245351.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane-associated ring finger (C3HC4) 6 (March6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245351.3

ORF Insert Sequence:

ATGGACACCG CCGAGGAAGA TATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTATC ATCCTTGTGT ATGTACTGGC AGTATTAAAT TTATTCATCA AGAATGCTTA 
GTTCAGTGGT TGAAACATAG TCGGAAAGAA TACTGTGAAT TATGCAAGCA CAGATTTGCT 
TTCACACCAA TTTATTCTCC AGATATGCCT TCACGGCTAC CAATCCAAGA CATATTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCAATA CGATACTGGT TTCATTACAC ACTTGTGGCC 
TTTGCATGGC TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCCGTGA GCTCACTACT GACACTGCCA CTAGACATGC TGTCAACGGA AAATTTGTTG 
GCGGATTGCT TGCAGGGCTG TTTTGTGGTG ACGTGCACAC TTTGTGCGTT CATCAGCCTG 
GTGTGGTTAC GAGAGCAGAT AGTCCACGGG GGAGCCCCAA TTTGGCTGGA GCATGCTGCT 
CCACCCTTCA ATGCTGCTGG ACACCACCAA AATGAGGCTC CTGTAGGAGG AAATGGTGCA 
GAAAACCCTG CTGCTGATCA GCCAGCTAAC CCAGCAGGTG AGAATGCAGT TCTGGGTGAA 
AACCCTGATG CCCAGGACGG TCAAGCAGAA GAGGAAGAGG AGGACAATGA GGAGGAAGAT 
GATGCGGGTG TAGAAGATGC TGCTGATGCC AACAATGGAG CCCAAGACGA CATGAACTGG 
AATGCTTTAG AGTGGGACCG GGCTGCAGAA GAGCTTACTT GGGAAAGAAT GCTTGGACTT 
GATGGATCAC TAGTTTTCCT GGAACACGTG TTCTGGGTGG TGTCTTTAAA TACATTGTTC 
ATTCTTGTTT TTGCATTTTG TCCTTACCAC ATCGGCCATT TCTCCCTTGT TGGCTTGGGG 
TTTGAGGAGC ATGTCCAAGC ATCTCATTTT GAAGGTCTAA TCACAACGAT TGTTGGATAT 
ATACTTTTAG CAATAACACT GATAATTTGT CATGCATTGG CAACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG CGTCTGCTAT ATTGTGGTTA AGGTCTCTTT GTTAGTTGTG 
GTAGAAATCG GAGTGTTCCC TCTCATTTGT GGCTGGTGGC TGGATATCTG TTCTCTGGAA 
ATGTTTGATG CTACTCTGAA AGATCGAGAG CTGAGCTTTC AGTCAGCTCC AGGGACCACG 
ATGTTTCTGC ACTGGTTGGT GGGAATGGTG TATGTGTTCT ACTTTGCCTC CTTCATCCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTC TTATGGTTTC TGAGGAATTT GAACGATCCA 
GATTTTAATC CAGTACAGGA AATGATTCAC TTGCCCATTT ATAGACATCT CCGAAGATTT 
ATTTTGTCAG TGATTGTCTT TGGCTCCATT GTCCTTCTGA TGCTCTGGCT TCCTATACGT 
ATAATTAAGA GTCTCTTGCC TAATTTTCTT CCTTACAATG TCATGCTCTA CAGTGATGCT 
CCGGTGAGTG AGCTGTCCCT TGAGCTGCTC CTACTGCAGG TTGTCTTGCC AGCATTGCTG 
GAACAGGGAC ACACGAGGCA GTGGCTGAAG GGGCTTGTGC GTGCATGGAC TGTTACTGCT 
GGATACTTGC TGGACCTTCA TTCCTACTTA CTGGGAGATC AGGAAGAAAA CGAGAACAGT 
GCAAATCAGC AGGTCAACAA TAACCAGCCT GCACGAAACA ATAATGCTGT TCCTGCCGGG 
GAAGGTCTGC ATGCAGCCCA CCAAGCCATA CTCCAGCAGG GAGGACCTGT TGGCTTTCAG 
CCTTACCGCC GGCCTTTAAA TTTCCCACTC AGGATATTTC TGCTGATTGT CTTCATGTGC 
ATAACATTGC TAATTGCCAG CCTCATTTGC CTTACTTTAC CAGCCTTTCT ATTTTCAGTA 
TTTGCTGGCC GTTGGTTAAT GTCATTTTGG ACGGGGACTG CCAAAATCCA TGAGCTGTAC 
ACAGCTGCTT GTGGTCTCTA TGTGTGTTGG CTAACCATAA GGGCTGTGAC GGTGCTGGTG 
GCCTGGATGC CCCAGGGACG AAGAGTGATT TTCCAGAAGG TTAAAGAGTG GTCCCTCATG 
ATAATGAAGA CGTTGATAGT TGCTGTGCTG CTGGCTGGGG TCGTCCCACT GCTCCTCGGG 
CTCCTGTTTG AGCTGGTTAT TGTTGCTCCT CTGAGGGTCC CTCTTGATCA GACTCCCCTT 
TTCTACCCTT GGCAGGACTG GGCGCTGGGA GTGCTGCATG CCAAAATTAT CGCAGCTATA 
ACTCTTATGG GTCCTCAGTG GTGGTTGAAA ACTGTCATTG AACAGGTTTA CGCAAATGGC 
ATTCGTAACA TCGACCTTCA CTATATCATC CGTAAGCTGG CAGCCCCGGT GATCTCTGTG 
CTCTTACTTT CCCTGTGTGT ACCGTATGTC ATAGCTTCTG GCGCGGTTCC TCTACTAGGT 
GTTACTGCAG AGATGCAGAA CTTAGTTCAC CGGCGGATTT ACCCATTTTT ATTGATGGTC 
GTTGTGCTGA TGGGAATCCT GTCCTTCCAG GTCCGCCAGT TTAAGCGCCT TTATGAACAC 
ATTAAAAATG ACAAGTACCT TGTGGGGCAG CGCCTGGTGA ACTATGAGCG CAAATCCGGC 
AAGCAAGGCC CGTCTACACC GCCTCCGGTG TCGTCCCAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tagging genes with cassette-exchange sites.
Nucleic acids research33(4)e44(2005 Mar)
Cobellis G,Nicolaus G,Iovino M,Romito A,Marra E,Barbarisi M,Sardiello M,Di Giorgio FP,Iovino N,Zollo M,Ballabio A,Cortese R

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Nagase T,Ohara O,Koga H

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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March6 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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