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Wars cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Wars
Entrez Gene ID 22375
Full Name tryptophanyl-tRNA synthetase
Synonyms TrpRS,WRS
General protein information
Preferred Names

tryptophanyl-tRNA synthetase

Names

tryptophan--tRNA ligase, cytoplasmic
tryptophanyl-tRNA synthetase, cytoplasmic
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

12

12|12 F1

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001164314.1 NP_001157786.1 tryptophan--tRNA ligase, cytoplasmic isoform 2
XM_006515819.1 XP_006515882.1 tryptophan--tRNA ligase, cytoplasmic isoform X1
NM_001164488.1 NP_001157960.1 tryptophan--tRNA ligase, cytoplasmic isoform 2
NM_011710.3 NP_035840.3 tryptophan--tRNA ligase, cytoplasmic isoform 1
XM_006515819.2 XP_006515882.1 tryptophan--tRNA ligase, cytoplasmic isoform X1

Pathways from BioSystems


Homologies


Gallus gallus (chicken) WARS XP_004936372.1
Danio rerio (zebrafish) wars NP_957066.1
Caenorhabditis elegans (roundworm) wars-1 NP_507683.2
Magnaporthe oryzae (rice blast fungus) MGG_09540 XP_003712201.1
Arabidopsis thaliana (thale cress) AT3G04600 NP_974219.1
Kluyveromyces lactis KLLA0B07733g XP_451874.1
Neurospora crassa NCU06722 XP_960902.1
Pan troglodytes (chimpanzee) WARS XP_003952625.1
Bos taurus (cattle) WARS NP_776643.1
Drosophila melanogaster (fruit fly) Aats-trp NP_524826.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP003315 XP_319546.5
Schizosaccharomyces pombe (fission yeast) wrs1 NP_592983.1
Oryza sativa (rice) Os12g0540900 NP_001066951.1
Homo sapiens (human) WARS NP_776049.1
WARS XP_001105786.1
Canis lupus familiaris (dog) WARS XP_537554.1
Mus musculus (house mouse) Wars NP_035840.3
Saccharomyces cerevisiae (baker's yeast) WRS1 NP_014544.1
Xenopus tropicalis (tropical clawed frog) wars NP_001039220.1
Rattus norvegicus (Norway rat) Wars NP_001013188.2
Eremothecium gossypii AGOS_ADR117W NP_984213.1

Gene Ontology


Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Species-specific differences in the regulation of the aminoacylation activity of mammalian tryptophanyl-tRNA synthetases.
Wakasugi K
FEBS letters584(1)229-32(2010 Jan)

Tryptamine induces tryptophanyl-tRNA synthetase-mediated neurodegeneration with neurofibrillary tangles in human cell and mouse models.
Paley EL, Denisova G, Sokolova O, Posternak N, Wang X, Brownell AL
Neuromolecular medicine9(1)55-82(2007)

Aberrant regulation of imprinted gene expression in Gtl2lacZ mice.
Sekita Y, Wagatsuma H, Irie M, Kobayashi S, Kohda T, Matsuda J, Yokoyama M, Ogura A, Schuster-Gossler K, Gossler A, Ishino F, Kaneko-Ishino T
Cytogenetic and genome research113(1-4)223-9(2006)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TrpRS plays a role in protecting against neurodegeneration is suggested, providing an insight into the pathogenesis and a possible treatment of neurodegenerative diseases.
Title: Tryptamine induces tryptophanyl-tRNA synthetase-mediated neurodegeneration with neurofibrillary tangles in human cell and mouse models.

The following Wars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu20337 NM_011710.3
Latest version!		 Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu20492 NM_001164314.1
Latest version!		 Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu20492 NM_001164488.1
Latest version!		 Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OMu20337 XM_006515819.2
Latest version!		 Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OMu20337 XM_006515819.1 Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu20337
Clone ID Related Accession (Same CDS sequence) XM_006515819.1 , NM_011710.3 , XM_006515819.2
Accession Version NM_011710.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-15
Organism Mus musculus(house mouse)
Product tryptophan--tRNA ligase, cytoplasmic isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122811.4, CD563585.1, CF731228.1 and AC152061.6. On Aug 29, 2009 this sequence version replaced NM_011710.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC003450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTGCTT CTGCTTTGAC 
ACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_035840.3
CDS115..1560
Translation

Target ORF information:

RefSeq Version NM_011710.3
Organism Mus musculus(house mouse)
Definition Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_011710.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTGCTT CTGCTTTGAC 
ACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu20492
Clone ID Related Accession (Same CDS sequence) NM_001164314.1 , NM_001164488.1
Accession Version NM_001164314.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-14
Organism Mus musculus(house mouse)
Product tryptophan--tRNA ligase, cytoplasmic isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY773669.1, AK152966.1, AC122811.4 and AC152061.6. Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The encoded isoform (2) has a shorter C-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: AK153337.1, AK150090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157786.1
CDS169..1596
Translation

Target ORF information:

RefSeq Version NM_001164314.1
Organism Mus musculus(house mouse)
Definition Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001164314.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu20492
Clone ID Related Accession (Same CDS sequence) NM_001164314.1 , NM_001164488.1
Accession Version NM_001164488.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-15
Organism Mus musculus(house mouse)
Product tryptophan--tRNA ligase, cytoplasmic isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK004541.1 and AC152061.6. Transcript Variant: This variant (3) differs in the 3' UTR and 3' coding region, compared to variant 1. The encoded isoform (2) has a shorter C-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: AK004541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157960.1
CDS102..1529
Translation

Target ORF information:

RefSeq Version NM_001164488.1
Organism Mus musculus(house mouse)
Definition Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001164488.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu20337
Clone ID Related Accession (Same CDS sequence) XM_006515819.1 , NM_011710.3 , XM_006515819.2
Accession Version XM_006515819.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product tryptophan--tRNA ligase, cytoplasmic isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTGCTT CTGCTTTGAC 
ACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006515882.1
CDS164..1609
Translation

Target ORF information:

RefSeq Version XM_006515819.1
Organism Mus musculus(house mouse)
Definition Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006515819.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTGCTT CTGCTTTGAC 
ACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu20337
Clone ID Related Accession (Same CDS sequence) XM_006515819.1 , NM_011710.3 , XM_006515819.2
Accession Version XM_006515819.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product tryptophan--tRNA ligase, cytoplasmic isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTGCTT CTGCTTTGAC 
ACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006515882.1
CDS170..1615
Translation

Target ORF information:

RefSeq Version XM_006515819.2
Organism Mus musculus(house mouse)
Definition Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006515819.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTGCTT CTGCTTTGAC 
ACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA


book

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H


book

Species-specific differences in the regulation of the aminoacylation activity of mammalian tryptophanyl-tRNA synthetases.
FEBS letters584(1)229-32(2010 Jan)
Wakasugi K


book

Tryptamine induces tryptophanyl-tRNA synthetase-mediated neurodegeneration with neurofibrillary tangles in human cell and mouse models.
Neuromolecular medicine9(1)55-82(2007)
Paley EL,Denisova G,Sokolova O,Posternak N,Wang X,Brownell AL


book

Aberrant regulation of imprinted gene expression in Gtl2lacZ mice.
Cytogenetic and genome research113(1-4)223-9(2006)
Sekita Y,Wagatsuma H,Irie M,Kobayashi S,Kohda T,Matsuda J,Yokoyama M,Ogura A,Schuster-Gossler K,Gossler A,Ishino F,Kaneko-Ishino T