Ndufs1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Ndufs1
Entrez Gene ID	227197
Full Name	NADH dehydrogenase (ubiquinone) Fe-S protein 1
Synonyms	5830412M15Rik,9930026A05Rik
General protein information	Preferred Names NADH dehydrogenase (ubiquinone) Fe-S protein 1 Names NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial CI-75kD complex I-75kD
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001160040.1	NP_001153512.1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor
NM_001160039.1	NP_001153511.1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor
XM_006495952.3	XP_006496015.1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1
NM_145518.2	NP_663493.2	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor
XM_006495952.4	XP_006496015.1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1
NM_001160038.1	NP_001153510.1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor
XM_030253694.1	XP_030109554.1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ndufs1	NP_001007766.1
Drosophila melanogaster (fruit fly)	ND75	NP_511083.1
Arabidopsis thaliana (thale cress)	EMB1467	NP_568550.1
Oryza sativa (rice)	Os03g0713400	NP_001051072.1
Xenopus tropicalis (tropical clawed frog)	ndufs1	XP_002936432.2
Neurospora crassa	NCU01765	XP_957188.2
Homo sapiens (human)	NDUFS1	NP_001186913.1
	NDUFS1	XP_001105816.2
Rattus norvegicus (Norway rat)	Ndufs1	NP_001005550.1
Caenorhabditis elegans (roundworm)	nuo-5	NP_503733.1
Canis lupus familiaris (dog)	NDUFS1	XP_005640630.1
Magnaporthe oryzae (rice blast fungus)	MGG_03155	XP_003716838.1
Pan troglodytes (chimpanzee)	NDUFS1	NP_001073384.1
Bos taurus (cattle)	NDUFS1	NP_777245.1
Mus musculus (house mouse)	Ndufs1	NP_001153510.1
Gallus gallus (chicken)	NDUFS1	NP_001006518.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001653	XP_003435895.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Conformational change of mitochondrial complex I increases ROS sensitivity during ischemia.
Gorenkova N, Robinson E, Grieve DJ, Galkin A
Antioxidants & redox signaling19(13)1459-68(2013 Nov)

TNF?-induced lysosomal membrane permeability is downstream of MOMP and triggered by caspase-mediated NDUFS1 cleavage and ROS formation.
Huai J, V?gtle FN, J?ckel L, Li Y, Kiefer T, Ricci JE, Borner C
Journal of cell science126(Pt 17)4015-25(2013 Sep)

Region-specific expression of mitochondrial complex I genes during murine brain development.
Wirtz S, Schuelke M
PloS one6(4)e18897(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The transition of mitochondrial complex I into the D-form in the absence of oxygen may represent a key event in promoting cardiac injury during I/R.
Title: Conformational change of mitochondrial complex I increases ROS sensitivity during ischemia.

lysosomal membrane permeability induced by TNFalpha plus cycloheximide, the release of lysosomal cathepsins and reactive oxygen species formation require the caspase-3-mediated cleavage of the p75 NDUFS1 subunit of respiratory complex I.
Title: TNF?-induced lysosomal membrane permeability is downstream of MOMP and triggered by caspase-mediated NDUFS1 cleavage and ROS formation.

The following Ndufs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndufs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18917	NM_001160039.1 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu18917	NM_001160040.1 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu18917	NM_001160038.1 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu18917	NM_145518.2 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu18917	XM_030253694.1 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu18917	XM_006495952.4 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu18917	XM_006495952.3	Mus musculus NADH dehydrogenase (ubiquinone) Fe-S protein 1 (Ndufs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndufs1 ( NM_001160040.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153512.1 Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu18917
Clone ID Related Accession (Same CDS sequence)	NM_001160040.1 , NM_001160039.1 , XM_006495952.3 , NM_145518.2 , XM_006495952.4 , NM_001160038.1 , XM_030253694.1
Accession Version	NM_001160040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY709888.1, BG808119.1, AK036926.1 and AV444601.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.100468.1, AK146814.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153512.1
CDS	401..2584
Translation	MLRIPIKRALIGLSNSPKGYVRTTGTAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNVIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQNFEGNAVAAIAGGLVDAEALVALKDLLNKVDSDNLCTEEIFPTEGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGRHSFCEVLKDAKKPMVVLGSSALQRDDGAAILVAVSNMVQKIRVTTGVAAEWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAPMADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGITLPYDTLDQVRNRLEEVSPNLVRYDDIEETNYFQQASELAKLVNQEVLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC

Target ORF information:

RefSeq Version	NM_001160040.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160040.1

ORF Insert Sequence:

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndufs1 ( NM_001160039.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153511.1 Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18917
Clone ID Related Accession (Same CDS sequence)	NM_001160040.1 , NM_001160039.1 , XM_006495952.3 , NM_145518.2 , XM_006495952.4 , NM_001160038.1 , XM_030253694.1
Accession Version	NM_001160039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY709888.1, CN715066.1, AK036926.1 and AV444601.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.249299.1, SRR6116558.42098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153511.1
CDS	347..2530
Translation	MLRIPIKRALIGLSNSPKGYVRTTGTAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNVIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQNFEGNAVAAIAGGLVDAEALVALKDLLNKVDSDNLCTEEIFPTEGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGRHSFCEVLKDAKKPMVVLGSSALQRDDGAAILVAVSNMVQKIRVTTGVAAEWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAPMADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGITLPYDTLDQVRNRLEEVSPNLVRYDDIEETNYFQQASELAKLVNQEVLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC

Target ORF information:

RefSeq Version	NM_001160039.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160039.1

ORF Insert Sequence:

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndufs1 ( NM_145518.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663493.2 Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant 4, mRNA.

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CloneID	OMu18917
Clone ID Related Accession (Same CDS sequence)	NM_001160040.1 , NM_001160039.1 , XM_006495952.3 , NM_145518.2 , XM_006495952.4 , NM_001160038.1 , XM_030253694.1
Accession Version	NM_145518.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY709888.1, AK036926.1 and AV444601.1. On May 9, 2009 this sequence version replaced NM_145518.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006660.1, AK142711.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663493.2
CDS	243..2426
Translation	MLRIPIKRALIGLSNSPKGYVRTTGTAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNVIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQNFEGNAVAAIAGGLVDAEALVALKDLLNKVDSDNLCTEEIFPTEGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGRHSFCEVLKDAKKPMVVLGSSALQRDDGAAILVAVSNMVQKIRVTTGVAAEWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAPMADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGITLPYDTLDQVRNRLEEVSPNLVRYDDIEETNYFQQASELAKLVNQEVLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC

Target ORF information:

RefSeq Version	NM_145518.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145518.2

ORF Insert Sequence:

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndufs1 ( NM_001160038.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153510.1 Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant 1, mRNA.

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CloneID	OMu18917
Clone ID Related Accession (Same CDS sequence)	NM_001160040.1 , NM_001160039.1 , XM_006495952.3 , NM_145518.2 , XM_006495952.4 , NM_001160038.1 , XM_030253694.1
Accession Version	NM_001160038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Mus musculus(house mouse)
Product	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY709888.1, BU708393.1, AK036926.1 and AV444601.1. Transcript Variant: This variant (1) represents the longest transcript. All four variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3224858.8518.1, SRR5189679.170577.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153510.1
CDS	505..2688
Translation	MLRIPIKRALIGLSNSPKGYVRTTGTAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNVIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQNFEGNAVAAIAGGLVDAEALVALKDLLNKVDSDNLCTEEIFPTEGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGRHSFCEVLKDAKKPMVVLGSSALQRDDGAAILVAVSNMVQKIRVTTGVAAEWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAPMADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGITLPYDTLDQVRNRLEEVSPNLVRYDDIEETNYFQQASELAKLVNQEVLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC

Target ORF information:

RefSeq Version	NM_001160038.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160038.1

ORF Insert Sequence:

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndufs1 ( XM_006495952.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496015.1 Mus musculus NADH dehydrogenase (ubiquinone) Fe-S protein 1 (Ndufs1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18917
Clone ID Related Accession (Same CDS sequence)	NM_001160040.1 , NM_001160039.1 , XM_006495952.3 , NM_145518.2 , XM_006495952.4 , NM_001160038.1 , XM_030253694.1
Accession Version	XM_006495952.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496015.1
CDS	322..2505
Translation	MLRIPIKRALIGLSNSPKGYVRTTGTAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNVIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQNFEGNAVAAIAGGLVDAEALVALKDLLNKVDSDNLCTEEIFPTEGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGRHSFCEVLKDAKKPMVVLGSSALQRDDGAAILVAVSNMVQKIRVTTGVAAEWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAPMADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGITLPYDTLDQVRNRLEEVSPNLVRYDDIEETNYFQQASELAKLVNQEVLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC

Target ORF information:

RefSeq Version	XM_006495952.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH dehydrogenase (ubiquinone) Fe-S protein 1 (Ndufs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495952.3

ORF Insert Sequence:

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndufs1 ( XM_006495952.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496015.1 Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18917
Clone ID Related Accession (Same CDS sequence)	NM_001160040.1 , NM_001160039.1 , XM_006495952.3 , NM_145518.2 , XM_006495952.4 , NM_001160038.1 , XM_030253694.1
Accession Version	XM_006495952.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495952.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496015.1
CDS	328..2511
Translation	MLRIPIKRALIGLSNSPKGYVRTTGTAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNVIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQNFEGNAVAAIAGGLVDAEALVALKDLLNKVDSDNLCTEEIFPTEGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGRHSFCEVLKDAKKPMVVLGSSALQRDDGAAILVAVSNMVQKIRVTTGVAAEWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAPMADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGITLPYDTLDQVRNRLEEVSPNLVRYDDIEETNYFQQASELAKLVNQEVLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC

Target ORF information:

RefSeq Version	XM_006495952.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495952.4

ORF Insert Sequence:

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndufs1 ( XM_030253694.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109554.1 Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu18917
Clone ID Related Accession (Same CDS sequence)	NM_001160040.1 , NM_001160039.1 , XM_006495952.3 , NM_145518.2 , XM_006495952.4 , NM_001160038.1 , XM_030253694.1
Accession Version	XM_030253694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109554.1
CDS	253..2436
Translation	MLRIPIKRALIGLSNSPKGYVRTTGTAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNVIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQNFEGNAVAAIAGGLVDAEALVALKDLLNKVDSDNLCTEEIFPTEGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGRHSFCEVLKDAKKPMVVLGSSALQRDDGAAILVAVSNMVQKIRVTTGVAAEWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAPMADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGITLPYDTLDQVRNRLEEVSPNLVRYDDIEETNYFQQASELAKLVNQEVLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC

Target ORF information:

RefSeq Version	XM_030253694.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253694.1

ORF Insert Sequence:

ATGTTAAGGA TACCTATAAA AAGGGCCTTG ATAGGCCTTT CTAATTCTCC TAAAGGATAT 
GTTCGCACAA CTGGCACAGC AGCAAGTAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC CACTGTTCTG CAGGCTTGCG AGAAGGTCGG CATGCAAATC 
CCTCGATTCT GTTACCATGA AAGGTTGTCT GTTGCTGGAA ATTGCAGGAT GTGCCTGGTA 
GAGATTGAGA AAGCTCCAAA GGTTGTCGCT GCTTGTGCTA TGCCTGTAAT GAAGGGCTGG 
AATATCTTGA CAAACTCGGA AAAATCTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCATTGGA TTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGATTTC TAGAGGGGAA GCGTGCTGTG 
GAGGACAAGA ACATTGGGCC CCTAGTAAAG ACCATCATGA CTAGATGCAT CCAGTGTACC 
CGGTGCATCA GGTTTGCAAG TGAGATTGCA GGAGTAGATG ATTTGGGAAC AACAGGAAGA 
GGAAATGACA TGCAAGTTGG CACATACATT GAAAAAATGT TTATGTCTGA ACTGTCTGGG 
AATGTCATTG ATATCTGCCC TGTAGGGGCC CTAACCTCTA AGCCTTATGC CTTTACTGCC 
CGGCCTTGGG AAACAAGAAA GACAGAGTCC ATTGATGTAA TGGATGCAGT GGGAAGTAAC 
ATTGTGGTTA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGAAT GCATGAAGAT 
ATTAATGAAG AATGGATCTC TGATAAAACC AGGTTTGCTT ATGATGGACT AAAACGTCAA 
AGACTTACTG AACCAATGGT CAGAAACGAA AAAGGGCTTT TAACTTATAC CTCCTGGGAA 
GATGCACTCT CTCGTGTAGC TGGAATGTTA CAGAATTTTG AAGGCAATGC TGTGGCAGCG 
ATTGCAGGAG GCTTGGTGGA TGCTGAAGCC TTAGTAGCTC TGAAAGACTT GCTTAATAAA 
GTTGACTCTG ACAACTTATG CACTGAAGAG ATCTTCCCCA CTGAAGGAGC TGGTACAGAC 
TTACGTTCCA ATTATCTTCT CAATACCACA ATTGCTGGTG TGGAAGAAGC AGATGTTGTT 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTGT TTAATGCTAG AATTAGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTCATCGGTA GTCCAGTGGA CCTCACTTAC 
AGATACGACC ATCTAGGAGA CTCTCCTAAA ATTCTGCAAG ACATTGCTTC AGGGAGGCAT 
TCATTCTGCG AGGTCTTAAA GGATGCTAAA AAACCAATGG TGGTTTTAGG CAGTTCTGCA 
CTCCAGAGAG ATGATGGAGC AGCAATTCTT GTAGCTGTGT CCAACATGGT ACAAAAGATT 
CGAGTGACAA CCGGTGTTGC TGCAGAGTGG AAAGTTATGA ATATTCTGCA TAGGATTGCA 
AGCCAGGTAG CTGCTTTGGA CCTTGGCTAT AAACCTGGGG TAGAAGCGAT TAGGAAGAAC 
CCTCCCAAAA TGCTGTTTCT TCTGGGAGCA GATGGAGGTT GTATCACGCG ACAGGACTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CACCATGGTG ATGTTGGTGC TCCCATGGCT 
GATGTTATTC TCCCAGGGGC TGCTTACACA GAAAAGTCTG CTACTTATGT CAATACTGAG 
GGCAGAGCTC AGCAAACCAA AGTAGCAGTG ACGCCTCCTG GCTTGGCACG AGAAGACTGG 
AAAATCATAA GAGCTCTCTC TGAGATTGCA GGTATCACTC TTCCATATGA CACTCTGGAT 
CAAGTAAGGA ACCGTCTTGA AGAGGTCTCT CCTAATCTGG TTCGATATGA TGATATTGAA 
GAAACTAATT ACTTTCAGCA AGCAAGTGAG CTTGCCAAGC TAGTAAACCA GGAGGTTCTT 
GCTGACCCAC TCGTTCCACC TCAGCTAACT ATAAAAGACT TCTATATGAC AGACTCCATT 
AGCAGAGCCT CACAGACAAT GGCCAAGTGT GTCAAAGCTG TCACCGAGGG CGCTCAGGCA 
GTAGAGGAGC CGTCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Conformational change of mitochondrial complex I increases ROS sensitivity during ischemia.
Antioxidants & redox signaling19(13)1459-68(2013 Nov)
Gorenkova N,Robinson E,Grieve DJ,Galkin A

TNF?-induced lysosomal membrane permeability is downstream of MOMP and triggered by caspase-mediated NDUFS1 cleavage and ROS formation.
Journal of cell science126(Pt 17)4015-25(2013 Sep)
Huai J,V?gtle FN,J?ckel L,Li Y,Kiefer T,Ricci JE,Borner C

Region-specific expression of mitochondrial complex I genes during murine brain development.
PloS one6(4)e18897(2011 Apr)
Wirtz S,Schuelke M

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Ndufs1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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