Wnk1 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Wnk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wnk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu81703 XM_017321542.2
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu81704 XM_017321543.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu81705 XM_017321544.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu81706 XM_017321545.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu81707 XM_017321546.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu81708 XM_017321547.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu81709 XM_017321548.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu81710 XM_017321550.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu81711 XM_017321552.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu81712 XM_017321553.2
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu81713 XM_017321555.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu81714 XM_017321556.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu89190 XM_030255355.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu15602 NM_198703.3
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu15715 NM_001185020.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu15743 NM_001185021.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu15955 NM_001199084.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu16018 NM_001199083.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu15602 XM_017321549.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu15715 XM_017321557.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu15743 XM_017321554.1
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu73608 XM_011241320.2
Latest version!
Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
View Old Accession Versions >>
OMu81712 XM_017321553.1 Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu81703 XM_017321542.1 Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu81703
    Clone ID Related Accession (Same CDS sequence) XM_017321542.1 , XM_017321542.2
    Accession Version XM_017321542.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT
    TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTATGTGCCC TCCAGCTGAA
    CCGAAATCAG GGACCAGCAG CACAAATACC GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC
    CAGGCTCAGC CTCCTGCTAT GACTTCCAGT AGGAAGGGCA CATTCACAGA TGACCTGCAC
    AAGTTAGTAG ACAACTGGGC CCGAGATGCC ATGAATCTTT CAGGCAGGAG AGGCAGCAAA
    GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG
    TGTGTTCCCA TGACCTCAAA CCTGGGTGGC TCTACCCCCA TCTCTGCAGC ATCTGCTACC
    TCTCTAGGTC ACTTCACCAA ATCCATGTGT CCCCCACAGC AGTATGGTTT TCCACCTGCC
    CCATTTGGCA CTCAGTGGAG TGGAACAGGT GGTCCAGCAC CGCAGCCACT TGGCCAGTTC
    CAACCTGTAG GAACAGCATC CTTGCAAAAT TTCAACATCA GCAATTTGCA GAAGTCCATC
    AGCAACCCCC CAGGTTCCAA TCTACGGACC ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177031.1
    CDS926..8221
    Translation

    Target ORF information:

    RefSeq Version XM_017321542.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT
    TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTATGTGCCC TCCAGCTGAA
    CCGAAATCAG GGACCAGCAG CACAAATACC GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC
    CAGGCTCAGC CTCCTGCTAT GACTTCCAGT AGGAAGGGCA CATTCACAGA TGACCTGCAC
    AAGTTAGTAG ACAACTGGGC CCGAGATGCC ATGAATCTTT CAGGCAGGAG AGGCAGCAAA
    GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG
    TGTGTTCCCA TGACCTCAAA CCTGGGTGGC TCTACCCCCA TCTCTGCAGC ATCTGCTACC
    TCTCTAGGTC ACTTCACCAA ATCCATGTGT CCCCCACAGC AGTATGGTTT TCCACCTGCC
    CCATTTGGCA CTCAGTGGAG TGGAACAGGT GGTCCAGCAC CGCAGCCACT TGGCCAGTTC
    CAACCTGTAG GAACAGCATC CTTGCAAAAT TTCAACATCA GCAATTTGCA GAAGTCCATC
    AGCAACCCCC CAGGTTCCAA TCTACGGACC ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81703
    Clone ID Related Accession (Same CDS sequence) XM_017321542.1 , XM_017321542.2
    Accession Version XM_017321542.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT
    TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTATGTGCCC TCCAGCTGAA
    CCGAAATCAG GGACCAGCAG CACAAATACC GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC
    CAGGCTCAGC CTCCTGCTAT GACTTCCAGT AGGAAGGGCA CATTCACAGA TGACCTGCAC
    AAGTTAGTAG ACAACTGGGC CCGAGATGCC ATGAATCTTT CAGGCAGGAG AGGCAGCAAA
    GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG
    TGTGTTCCCA TGACCTCAAA CCTGGGTGGC TCTACCCCCA TCTCTGCAGC ATCTGCTACC
    TCTCTAGGTC ACTTCACCAA ATCCATGTGT CCCCCACAGC AGTATGGTTT TCCACCTGCC
    CCATTTGGCA CTCAGTGGAG TGGAACAGGT GGTCCAGCAC CGCAGCCACT TGGCCAGTTC
    CAACCTGTAG GAACAGCATC CTTGCAAAAT TTCAACATCA GCAATTTGCA GAAGTCCATC
    AGCAACCCCC CAGGTTCCAA TCTACGGACC ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177031.1
    CDS942..8237
    Translation

    Target ORF information:

    RefSeq Version XM_017321542.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321542.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT
    TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTATGTGCCC TCCAGCTGAA
    CCGAAATCAG GGACCAGCAG CACAAATACC GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC
    CAGGCTCAGC CTCCTGCTAT GACTTCCAGT AGGAAGGGCA CATTCACAGA TGACCTGCAC
    AAGTTAGTAG ACAACTGGGC CCGAGATGCC ATGAATCTTT CAGGCAGGAG AGGCAGCAAA
    GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG
    TGTGTTCCCA TGACCTCAAA CCTGGGTGGC TCTACCCCCA TCTCTGCAGC ATCTGCTACC
    TCTCTAGGTC ACTTCACCAA ATCCATGTGT CCCCCACAGC AGTATGGTTT TCCACCTGCC
    CCATTTGGCA CTCAGTGGAG TGGAACAGGT GGTCCAGCAC CGCAGCCACT TGGCCAGTTC
    CAACCTGTAG GAACAGCATC CTTGCAAAAT TTCAACATCA GCAATTTGCA GAAGTCCATC
    AGCAACCCCC CAGGTTCCAA TCTACGGACC ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81704
    Clone ID Related Accession (Same CDS sequence) XM_017321543.1
    Accession Version XM_017321543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GGAAGATGGT GAAAAAAGTC
    TGTCCTTGCA ACCAGCTCTG TATGTGCCCT CCAGCTGAAC CGAAATCAGG GACCAGCAGC
    ACAAATACCG TTGGAGGAAC AGTGAGTAGC CAGGCTGCCC AGGCTCAGCC TCCTGCTATG
    ACTTCCAGTA GGAAGGGCAC ATTCACAGAT GACCTGCACA AGTTAGTAGA CAACTGGGCC
    CGAGATGCCA TGAATCTTTC AGGCAGGAGA GGCAGCAAAG GACACATGAA TTATGAGGGC
    CCTGGAATGG CAAGGAAGTT CTCTGCTCCT GGGCAGCTGT GTGTTCCCAT GACCTCAAAC
    CTGGGTGGCT CTACCCCCAT CTCTGCAGCA TCTGCTACCT CTCTAGGTCA CTTCACCAAA
    TCCATGTGTC CCCCACAGCA GTATGGTTTT CCACCTGCCC CATTTGGCAC TCAGTGGAGT
    GGAACAGGTG GTCCAGCACC GCAGCCACTT GGCCAGTTCC AACCTGTAGG AACAGCATCC
    TTGCAAAATT TCAACATCAG CAATTTGCAG AAGTCCATCA GCAACCCCCC AGGTTCCAAT
    CTACGGACCA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177032.1
    CDS885..8159
    Translation

    Target ORF information:

    RefSeq Version XM_017321543.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GGAAGATGGT GAAAAAAGTC
    TGTCCTTGCA ACCAGCTCTG TATGTGCCCT CCAGCTGAAC CGAAATCAGG GACCAGCAGC
    ACAAATACCG TTGGAGGAAC AGTGAGTAGC CAGGCTGCCC AGGCTCAGCC TCCTGCTATG
    ACTTCCAGTA GGAAGGGCAC ATTCACAGAT GACCTGCACA AGTTAGTAGA CAACTGGGCC
    CGAGATGCCA TGAATCTTTC AGGCAGGAGA GGCAGCAAAG GACACATGAA TTATGAGGGC
    CCTGGAATGG CAAGGAAGTT CTCTGCTCCT GGGCAGCTGT GTGTTCCCAT GACCTCAAAC
    CTGGGTGGCT CTACCCCCAT CTCTGCAGCA TCTGCTACCT CTCTAGGTCA CTTCACCAAA
    TCCATGTGTC CCCCACAGCA GTATGGTTTT CCACCTGCCC CATTTGGCAC TCAGTGGAGT
    GGAACAGGTG GTCCAGCACC GCAGCCACTT GGCCAGTTCC AACCTGTAGG AACAGCATCC
    TTGCAAAATT TCAACATCAG CAATTTGCAG AAGTCCATCA GCAACCCCCC AGGTTCCAAT
    CTACGGACCA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81705
    Clone ID Related Accession (Same CDS sequence) XM_017321544.1
    Accession Version XM_017321544.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT
    TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTAGGACCAG CAGCACAAAT
    ACCGTTGGAG GAACAGTGAG TAGCCAGGCT GCCCAGGCTC AGCCTCCTGC TATGACTTCC
    AGTAGGAAGG GCACATTCAC AGATGACCTG CACAAGTTAG TAGACAACTG GGCCCGAGAT
    GCCATGAATC TTTCAGGCAG GAGAGGCAGC AAAGGACACA TGAATTATGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC TCCTGGGCAG CTGTGTGTTC CCATGACCTC AAACCTGGGT
    GGCTCTACCC CCATCTCTGC AGCATCTGCT ACCTCTCTAG GTCACTTCAC CAAATCCATG
    TGTCCCCCAC AGCAGTATGG TTTTCCACCT GCCCCATTTG GCACTCAGTG GAGTGGAACA
    GGTGGTCCAG CACCGCAGCC ACTTGGCCAG TTCCAACCTG TAGGAACAGC ATCCTTGCAA
    AATTTCAACA TCAGCAATTT GCAGAAGTCC ATCAGCAACC CCCCAGGTTC CAATCTACGG
    ACCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177033.1
    CDS885..8153
    Translation

    Target ORF information:

    RefSeq Version XM_017321544.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321544.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT
    TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTAGGACCAG CAGCACAAAT
    ACCGTTGGAG GAACAGTGAG TAGCCAGGCT GCCCAGGCTC AGCCTCCTGC TATGACTTCC
    AGTAGGAAGG GCACATTCAC AGATGACCTG CACAAGTTAG TAGACAACTG GGCCCGAGAT
    GCCATGAATC TTTCAGGCAG GAGAGGCAGC AAAGGACACA TGAATTATGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC TCCTGGGCAG CTGTGTGTTC CCATGACCTC AAACCTGGGT
    GGCTCTACCC CCATCTCTGC AGCATCTGCT ACCTCTCTAG GTCACTTCAC CAAATCCATG
    TGTCCCCCAC AGCAGTATGG TTTTCCACCT GCCCCATTTG GCACTCAGTG GAGTGGAACA
    GGTGGTCCAG CACCGCAGCC ACTTGGCCAG TTCCAACCTG TAGGAACAGC ATCCTTGCAA
    AATTTCAACA TCAGCAATTT GCAGAAGTCC ATCAGCAACC CCCCAGGTTC CAATCTACGG
    ACCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81706
    Clone ID Related Accession (Same CDS sequence) XM_017321545.1
    Accession Version XM_017321545.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GGAAGATGGT GAAAAAAGTC
    TGTCCTTGCA ACCAGCTCTG TAGGACCAGC AGCACAAATA CCGTTGGAGG AACAGTGAGT
    AGCCAGGCTG CCCAGGCTCA GCCTCCTGCT ATGACTTCCA GTAGGAAGGG CACATTCACA
    GATGACCTGC ACAAGTTAGT AGACAACTGG GCCCGAGATG CCATGAATCT TTCAGGCAGG
    AGAGGCAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT
    CCTGGGCAGC TGTGTGTTCC CATGACCTCA AACCTGGGTG GCTCTACCCC CATCTCTGCA
    GCATCTGCTA CCTCTCTAGG TCACTTCACC AAATCCATGT GTCCCCCACA GCAGTATGGT
    TTTCCACCTG CCCCATTTGG CACTCAGTGG AGTGGAACAG GTGGTCCAGC ACCGCAGCCA
    CTTGGCCAGT TCCAACCTGT AGGAACAGCA TCCTTGCAAA ATTTCAACAT CAGCAATTTG
    CAGAAGTCCA TCAGCAACCC CCCAGGTTCC AATCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177034.1
    CDS885..8132
    Translation

    Target ORF information:

    RefSeq Version XM_017321545.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321545.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG
    GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GGAAGATGGT GAAAAAAGTC
    TGTCCTTGCA ACCAGCTCTG TAGGACCAGC AGCACAAATA CCGTTGGAGG AACAGTGAGT
    AGCCAGGCTG CCCAGGCTCA GCCTCCTGCT ATGACTTCCA GTAGGAAGGG CACATTCACA
    GATGACCTGC ACAAGTTAGT AGACAACTGG GCCCGAGATG CCATGAATCT TTCAGGCAGG
    AGAGGCAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT
    CCTGGGCAGC TGTGTGTTCC CATGACCTCA AACCTGGGTG GCTCTACCCC CATCTCTGCA
    GCATCTGCTA CCTCTCTAGG TCACTTCACC AAATCCATGT GTCCCCCACA GCAGTATGGT
    TTTCCACCTG CCCCATTTGG CACTCAGTGG AGTGGAACAG GTGGTCCAGC ACCGCAGCCA
    CTTGGCCAGT TCCAACCTGT AGGAACAGCA TCCTTGCAAA ATTTCAACAT CAGCAATTTG
    CAGAAGTCCA TCAGCAACCC CCCAGGTTCC AATCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81707
    Clone ID Related Accession (Same CDS sequence) XM_017321546.1
    Accession Version XM_017321546.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGCC TTTGTCTACG ATGTCTTCAA CAACAGTTAC AGAAGCAGGG
    ACTCGGCTTC AAAAGGATGG CACAGAAGGT CATGTCACAG CAACTAGTTC AGGAGCTGGT
    GTTGTTAAGA TGGGGCGATT TCAGGTTTCA GTTACAATGG ATGATGCCCA GAAAGAACGT
    AAAAATAGGT CAGAAGATAC AAAGTCGGTT CATTTTGAAT CTAGCACATC AGAGTCATCA
    GTTCTTTCAA GTAGTAGTCC AGAGAGTACC CTGGTAAAGC CAGAGCCTAA TGGAATTAGT
    ATTTCTGGCA TCTCCTTAGA TGTCCCAGAC AGCACCCACA AAGCACCTAC CCCAGAAGCA
    AAGTCAGATG CAGGACAGCC TACCAAGGTG GGACGTTTTC AGGTGACAAC TACAGCCAAC
    AAAGTGGGTC GTTTCTCTGT TTCAAGAACT GAGGACAAGG TCACAGAGCT CAAAAAAGAG
    GGACCAGTGA CCTCTCCTCC TTTCAGAGAT TCAGAACAAA CTGTTATTCC TGCTGTGATA
    CCAAAGAAGG AGAAGCCTGA GCTGGCAGAA CCTTCCCATT TGAATGGGCC ATCTTCAGAC
    CTGGAGGCTG CCTTTCTAAG TAGGGGTACA GAGGATGGCT CAGGTAGTCC ACACTCTCCC
    CCTCACCTGT GTTCAAAGAG CCTTCCCGTC CAGAATCTAA GTCAAAGCCT TAGTAATTCA
    TTCAACTCCT CATACATGAG TAGTGACAAT GAGTCCGACA TTGAAGATGA AGACTTAAGG
    TTAGAACTAC GACGACTACG AGAAAAACAT CTTAAAGAGA TTCAGGACCT GCAGAGTCGC
    CAGAAGCATG AAATTGAATC TTTGTATACT AAACTGGGCA AGGTTCCCCC TGCTGTCATT
    ATTCCCCCAG CTGCTCCTCT GTCGGGGAGA AGAAGGAGAC CCACTAAAAG CAAAGGCAGC
    AAGTCTAGTC GCAGCAGCTC ATTGGGCAAT AAAAGCCCAC AGCTTTCAGG CAACCTGTCT
    GGTCAGAGTG GAACTTCAGT CTTACACCCC CAACAGACCC TCCATCCCGC AGGAAACACC
    CCCGAGACTG GGCACAACCA GCTGTTACAG CCTCTTAAGC CATCTCCCTC CAGTGACAAC
    CTCTATTCAG CCTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGT
    CAAGGCTGTG CGAAGTTTAA CTGTGCATCT GAGCAGGTGA CATTCAAACC TGGTGGCAGG
    AGGACCCGAT TTCTGAGTAC GCCCTGCTTG GCTCTTTGGA AGATGGTGAA AAAAGTCTGT
    CCTTGCAACC AGCTCTGTAT GTGCCCTCCA GCTGAACCGA AATCAGGGAC CAGCAGCACA
    AATACCGTTG GAGGAACAGT GAGTAGCCAG GCTGCCCAGG CTCAGCCTCC TGCTATGACT
    TCCAGTAGGA AGGGCACATT CACAGATGAC CTGCACAAGT TAGTAGACAA CTGGGCCCGA
    GATGCCATGA ATCTTTCAGG CAGGAGAGGC AGCAAAGGAC ACATGAATTA TGAGGGCCCT
    GGAATGGCAA GGAAGTTCTC TGCTCCTGGG CAGCTGTGTG TTCCCATGAC CTCAAACCTG
    GGTGGCTCTA CCCCCATCTC TGCAGCATCT GCTACCTCTC TAGGTCACTT CACCAAATCC
    ATGTGTCCCC CACAGCAGTA TGGTTTTCCA CCTGCCCCAT TTGGCACTCA GTGGAGTGGA
    ACAGGTGGTC CAGCACCGCA GCCACTTGGC CAGTTCCAAC CTGTAGGAAC AGCATCCTTG
    CAAAATTTCA ACATCAGCAA TTTGCAGAAG TCCATCAGCA ACCCCCCAGG TTCCAATCTA
    CGGACCACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177035.1
    CDS885..8096
    Translation

    Target ORF information:

    RefSeq Version XM_017321546.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321546.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGCC TTTGTCTACG ATGTCTTCAA CAACAGTTAC AGAAGCAGGG
    ACTCGGCTTC AAAAGGATGG CACAGAAGGT CATGTCACAG CAACTAGTTC AGGAGCTGGT
    GTTGTTAAGA TGGGGCGATT TCAGGTTTCA GTTACAATGG ATGATGCCCA GAAAGAACGT
    AAAAATAGGT CAGAAGATAC AAAGTCGGTT CATTTTGAAT CTAGCACATC AGAGTCATCA
    GTTCTTTCAA GTAGTAGTCC AGAGAGTACC CTGGTAAAGC CAGAGCCTAA TGGAATTAGT
    ATTTCTGGCA TCTCCTTAGA TGTCCCAGAC AGCACCCACA AAGCACCTAC CCCAGAAGCA
    AAGTCAGATG CAGGACAGCC TACCAAGGTG GGACGTTTTC AGGTGACAAC TACAGCCAAC
    AAAGTGGGTC GTTTCTCTGT TTCAAGAACT GAGGACAAGG TCACAGAGCT CAAAAAAGAG
    GGACCAGTGA CCTCTCCTCC TTTCAGAGAT TCAGAACAAA CTGTTATTCC TGCTGTGATA
    CCAAAGAAGG AGAAGCCTGA GCTGGCAGAA CCTTCCCATT TGAATGGGCC ATCTTCAGAC
    CTGGAGGCTG CCTTTCTAAG TAGGGGTACA GAGGATGGCT CAGGTAGTCC ACACTCTCCC
    CCTCACCTGT GTTCAAAGAG CCTTCCCGTC CAGAATCTAA GTCAAAGCCT TAGTAATTCA
    TTCAACTCCT CATACATGAG TAGTGACAAT GAGTCCGACA TTGAAGATGA AGACTTAAGG
    TTAGAACTAC GACGACTACG AGAAAAACAT CTTAAAGAGA TTCAGGACCT GCAGAGTCGC
    CAGAAGCATG AAATTGAATC TTTGTATACT AAACTGGGCA AGGTTCCCCC TGCTGTCATT
    ATTCCCCCAG CTGCTCCTCT GTCGGGGAGA AGAAGGAGAC CCACTAAAAG CAAAGGCAGC
    AAGTCTAGTC GCAGCAGCTC ATTGGGCAAT AAAAGCCCAC AGCTTTCAGG CAACCTGTCT
    GGTCAGAGTG GAACTTCAGT CTTACACCCC CAACAGACCC TCCATCCCGC AGGAAACACC
    CCCGAGACTG GGCACAACCA GCTGTTACAG CCTCTTAAGC CATCTCCCTC CAGTGACAAC
    CTCTATTCAG CCTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGT
    CAAGGCTGTG CGAAGTTTAA CTGTGCATCT GAGCAGGTGA CATTCAAACC TGGTGGCAGG
    AGGACCCGAT TTCTGAGTAC GCCCTGCTTG GCTCTTTGGA AGATGGTGAA AAAAGTCTGT
    CCTTGCAACC AGCTCTGTAT GTGCCCTCCA GCTGAACCGA AATCAGGGAC CAGCAGCACA
    AATACCGTTG GAGGAACAGT GAGTAGCCAG GCTGCCCAGG CTCAGCCTCC TGCTATGACT
    TCCAGTAGGA AGGGCACATT CACAGATGAC CTGCACAAGT TAGTAGACAA CTGGGCCCGA
    GATGCCATGA ATCTTTCAGG CAGGAGAGGC AGCAAAGGAC ACATGAATTA TGAGGGCCCT
    GGAATGGCAA GGAAGTTCTC TGCTCCTGGG CAGCTGTGTG TTCCCATGAC CTCAAACCTG
    GGTGGCTCTA CCCCCATCTC TGCAGCATCT GCTACCTCTC TAGGTCACTT CACCAAATCC
    ATGTGTCCCC CACAGCAGTA TGGTTTTCCA CCTGCCCCAT TTGGCACTCA GTGGAGTGGA
    ACAGGTGGTC CAGCACCGCA GCCACTTGGC CAGTTCCAAC CTGTAGGAAC AGCATCCTTG
    CAAAATTTCA ACATCAGCAA TTTGCAGAAG TCCATCAGCA ACCCCCCAGG TTCCAATCTA
    CGGACCACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81708
    Clone ID Related Accession (Same CDS sequence) XM_017321547.1
    Accession Version XM_017321547.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC
    CAGCTCTGTA TGTGCCCTCC AGCTGAACCG AAATCAGGGA CCAGCAGCAC AAATACCGTT
    GGAGGAACAG TGAGTAGCCA GGCTGCCCAG GCTCAGCCTC CTGCTATGAC TTCCAGTAGG
    AAGGGCACAT TCACAGATGA CCTGCACAAG TTAGTAGACA ACTGGGCCCG AGATGCCATG
    AATCTTTCAG GCAGGAGAGG CAGCAAAGGA CACATGAATT ATGAGGGCCC TGGAATGGCA
    AGGAAGTTCT CTGCTCCTGG GCAGCTGTGT GTTCCCATGA CCTCAAACCT GGGTGGCTCT
    ACCCCCATCT CTGCAGCATC TGCTACCTCT CTAGGTCACT TCACCAAATC CATGTGTCCC
    CCACAGCAGT ATGGTTTTCC ACCTGCCCCA TTTGGCACTC AGTGGAGTGG AACAGGTGGT
    CCAGCACCGC AGCCACTTGG CCAGTTCCAA CCTGTAGGAA CAGCATCCTT GCAAAATTTC
    AACATCAGCA ATTTGCAGAA GTCCATCAGC AACCCCCCAG GTTCCAATCT ACGGACCACC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177036.1
    CDS885..8087
    Translation

    Target ORF information:

    RefSeq Version XM_017321547.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321547.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC
    CAGCTCTGTA TGTGCCCTCC AGCTGAACCG AAATCAGGGA CCAGCAGCAC AAATACCGTT
    GGAGGAACAG TGAGTAGCCA GGCTGCCCAG GCTCAGCCTC CTGCTATGAC TTCCAGTAGG
    AAGGGCACAT TCACAGATGA CCTGCACAAG TTAGTAGACA ACTGGGCCCG AGATGCCATG
    AATCTTTCAG GCAGGAGAGG CAGCAAAGGA CACATGAATT ATGAGGGCCC TGGAATGGCA
    AGGAAGTTCT CTGCTCCTGG GCAGCTGTGT GTTCCCATGA CCTCAAACCT GGGTGGCTCT
    ACCCCCATCT CTGCAGCATC TGCTACCTCT CTAGGTCACT TCACCAAATC CATGTGTCCC
    CCACAGCAGT ATGGTTTTCC ACCTGCCCCA TTTGGCACTC AGTGGAGTGG AACAGGTGGT
    CCAGCACCGC AGCCACTTGG CCAGTTCCAA CCTGTAGGAA CAGCATCCTT GCAAAATTTC
    AACATCAGCA ATTTGCAGAA GTCCATCAGC AACCCCCCAG GTTCCAATCT ACGGACCACC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81709
    Clone ID Related Accession (Same CDS sequence) XM_017321548.1
    Accession Version XM_017321548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
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    3721
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC
    CAGCTCTGTA GGACCAGCAG CACAAATACC GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC
    CAGGCTCAGC CTCCTGCTAT GACTTCCAGT AGGAAGGGCA CATTCACAGA TGACCTGCAC
    AAGTTAGTAG ACAACTGGGC CCGAGATGCC ATGAATCTTT CAGGCAGGAG AGGCAGCAAA
    GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG
    TGTGTTCCCA TGACCTCAAA CCTGGGTGGC TCTACCCCCA TCTCTGCAGC ATCTGCTACC
    TCTCTAGGTC ACTTCACCAA ATCCATGTGT CCCCCACAGC AGTATGGTTT TCCACCTGCC
    CCATTTGGCA CTCAGTGGAG TGGAACAGGT GGTCCAGCAC CGCAGCCACT TGGCCAGTTC
    CAACCTGTAG GAACAGCATC CTTGCAAAAT TTCAACATCA GCAATTTGCA GAAGTCCATC
    AGCAACCCCC CAGGTTCCAA TCTACGGACC ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177037.1
    CDS885..8060
    Translation

    Target ORF information:

    RefSeq Version XM_017321548.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC
    CAGCTCTGTA GGACCAGCAG CACAAATACC GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC
    CAGGCTCAGC CTCCTGCTAT GACTTCCAGT AGGAAGGGCA CATTCACAGA TGACCTGCAC
    AAGTTAGTAG ACAACTGGGC CCGAGATGCC ATGAATCTTT CAGGCAGGAG AGGCAGCAAA
    GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG
    TGTGTTCCCA TGACCTCAAA CCTGGGTGGC TCTACCCCCA TCTCTGCAGC ATCTGCTACC
    TCTCTAGGTC ACTTCACCAA ATCCATGTGT CCCCCACAGC AGTATGGTTT TCCACCTGCC
    CCATTTGGCA CTCAGTGGAG TGGAACAGGT GGTCCAGCAC CGCAGCCACT TGGCCAGTTC
    CAACCTGTAG GAACAGCATC CTTGCAAAAT TTCAACATCA GCAATTTGCA GAAGTCCATC
    AGCAACCCCC CAGGTTCCAA TCTACGGACC ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81710
    Clone ID Related Accession (Same CDS sequence) XM_017321550.1
    Accession Version XM_017321550.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC CTGTGAATCA GCTGCAGAGT CAGGTTCACC CACAGCTTCT ACAGCCAACA
    ACAGTTCAGT CCATAGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGG TCCACTTCCC
    TCTGGAGATG TTTTGTACCA GGGCTTCCCA TCTCGACTGC CACCACAATA CCCAGGAGAT
    TCAAATATTG CTCCCTCTTC CAACGTGGCT TCTGTTTGCA TCCATTCTAC AGTCCTAGCC
    CCTCCTTCTA TGCCGACAGA AGCATTGGCC ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG
    CCTTATGTGG AATCAACTCC TTTGGTTCCT ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG
    TCCCAACCAG CAGTGAGTTT AACGCAACAA CCCCCAACTA CATCCTCCCA GCAAGCAGTT
    CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT GCTCCTCCAG AACAAACTCC AATAACACAG
    TCACAACCTA CTCAGCCTGT TCCATTGGTT ACCTCTGCAG ACAGTGCACA TTCAGACGTT
    GCTTCTGGTA TGAGTGATGG CAATGAGAAT GCTCCATCAT CCAGCGGGAG GCATGAAGGG
    AGGACAACAA AACGGCATTA TAGAAAATCC GTAAGAAGTC GCTCTCGGCA TGAAAAGACT
    TCTCGCCCAA AACTGAGGAT TTTAAATGTT TCAAATAAAG GAGACCGAGT AGTAGAATGT
    CAATTAGAGA CTCATAATCG GAAGATGGTT ACATTTAAAT TTGACCTAGA TGGTGACAAC
    CCTGAAGAGA TAGCAACAAT TATGGTGAAC AATGACTTTA TTCTAGCAAT AGAGAGAGAG
    TCTTTTGTGG CTCAAGTGCG GGAAATTATT GAAAAGGCTG ATGAAATGTT AAGTGAGGAT
    GTCAGTGTGG AGCCTGAGGG TGACCAGGGA TTGGAGAGTC TGCAAGGAAA GGATGACTAT
    GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA GAGTTCAAGC AACCAATTGC TGTATCTTCC
    ATGCCACAAC AAATAGGTGT TCCTACCAGT TCTTTAACGC AAGTTGTTCA TTCTGCTGGA
    AGACGGTTTA TAGTGAGTCC TGTACCTGAA AGTAGGTTAC GAGAATCAAA AGTTTTCACC
    AGTGACATAT CAGATCCAGT TGTGGCCTCT ACATCTCAGG CCCCTGGAAT GAACTTATCT
    CACTCTGCTT CATCCCTCAG TCTCCAGCAG GCTTTTTCTG AACTGAAACA TGGTCAGATG
    ACTGAAGGAC CCAATACAGC ACCTCCAAAT TTTAATCACA TGGCGGGACC AACATTTTCT
    CCCTTTTTGG CTAGCATTGC TGGTGTCCAA ACTGTAGCAG CATCCACACC GTCAGTATCA
    GTCCCTATAA CAAGCAGTCC TCTTAATGAC ATTTCCACAT CAGTGATGCA GTCTGAGACT
    GCACTGCCCA CTGAAAAAGG CATCGTGGGA GTTACCACTA CCAGCACAGG TGTGGTAGCT
    TCAGGTGGTC TCACCACAAT GTCTGTGTCT GAGTCACCAA CATCTTCCAG TGCGGTTTCA
    AGTTCTACAG TACCTGCAGT TGTCACTGTG TCCACACCAT CCCAGCCAGT TCAGGCTTCC
    ACATCTGGGA GCATTGCTTC AAGTACAGGC AGTTTTCCTC CTGGAACATT TTCTACAACT
    ACAGCTACTA CAATGGGTAG TGTGGTGGCC CCAGATGCTA AGCCTCCAAC TGTATTATTG
    CAACAGGTAG CAAGCAATAC TGCTGGGGTT GCCATAGTGA CATCAGTCTC TACCACCACT
    CCATTCCCAG GCATGGCTTC ACAGCCATCC CTTCCACTTA GTAGCAGCAC CTCTGCTCCT
    ACTCTAGCTG AAACAATGGT GGTTAGTGCA CACTCACTAG ACAAGGCATC TCACAGTAGT
    ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG CCATCCTCTT CCTCCTCCTC TGGAACAGCT
    GTGTCTACTT CTGTTTCTCA GCCTGGTATG GTTCATCCTT TGGTCATCTC ATCAGCTGTA
    GTTTCTACAC CTGGCCTCCC CCAACCAGTA GTACCTACTT CTACACCTTT ATTACCCCAA
    GTACCTAATA TCCCACCCTT GGTACAACCT GTTGTTAATG TGCCTGCTGT ACAGCAAACA
    CTCATTCACA GTCAGCCTCA ACCAGCTCTG CTTCCCAACC AGCCTCACAC ACACTGTCCT
    GAAATGGATG CTGATACCCA ATCCAAAGCT CCTGGGATTG ATGATATAAA GACTCTGGAG
    GAAAAGCTGA GATCTCTCTT TAGTGAACAC AGCTCATCTG GAACTCAGCA TGCTTCTGTC
    TCCCTAGAGA CACCTTTAGT AGTAGAGACG ACTGTCACAC CGGGCATCAC TACCACTGCT
    GTAGCACCAA GTAAACTCAT GACTTCCACT ACAAGTACTT GCTTACCACC AACCAGTTTA
    CCATTAGGAG CAGCTGGCAT GCCAGTTATG CCAGTAGGCA CACCTGGGCA GGTTTCTACC
    CCAGGCACAC ACGCTTCAGC CCCAGTCGGC ACTGCAACAG GAGTAAAACC TGGAACAACT
    CCCCCAAAGC CAACTAAGAC TGTGGTGCCA CCAGTGGGTA CTGAACTTTC AGCCGGTACT
    GTACCCTGTG AGCAGCTGCC ACCTTTTCCT GGACCTTCTC TAATTCAGTC CCAGCAACCA
    CTAGAAGATC TTGATGCTCA GTTGAGAAGA ACACTTAGTC CAGAGACTAT TACAGTGGCA
    CCTGCAGTTG GTCCTTTGTC TACGATGTCT TCAACAACAG TTACAGAAGC AGGGACTCGG
    CTTCAAAAGG ATGGCACAGA AGGTCATGTC ACAGCAACTA GTTCAGGAGC TGGTGTTGTT
    AAGATGGGGC GATTTCAGGT TTCAGTTACA ATGGATGATG CCCAGAAAGA ACGTAAAAAT
    AGGTCAGAAG ATACAAAGTC GGTTCATTTT GAATCTAGCA CATCAGAGTC ATCAGTTCTT
    TCAAGTAGTA GTCCAGAGAG TACCCTGGTA AAGCCAGAGC CTAATGGAAT TAGTATTTCT
    GGCATCTCCT TAGATGTCCC AGACAGCACC CACAAAGCAC CTACCCCAGA AGCAAAGTCA
    GATGCAGGAC AGCCTACCAA GGTGGGACGT TTTCAGGTGA CAACTACAGC CAACAAAGTG
    GGTCGTTTCT CTGTTTCAAG AACTGAGGAC AAGGTCACAG AGCTCAAAAA AGAGGGACCA
    GTGACCTCTC CTCCTTTCAG AGATTCAGAA CAAACTGTTA TTCCTGCTGT GATACCAAAG
    AAGGAGAAGC CTGAGCTGGC AGAACCTTCC CATTTGAATG GGCCATCTTC AGACCTGGAG
    GCTGCCTTTC TAAGTAGGGG TACAGAGGAT GGCTCAGGTA GTCCACACTC TCCCCCTCAC
    CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCATACA TGAGTAGTGA CAATGAGTCC GACATTGAAG ATGAAGACTT AAGGTTAGAA
    CTACGACGAC TACGAGAAAA ACATCTTAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACTAAACTG GGCAAGGTTC CCCCTGCTGT CATTATTCCC
    CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG AGACCCACTA AAAGCAAAGG CAGCAAGTCT
    AGTCGCAGCA GCTCATTGGG CAATAAAAGC CCACAGCTTT CAGGCAACCT GTCTGGTCAG
    AGTGGAACTT CAGTCTTACA CCCCCAACAG ACCCTCCATC CCGCAGGAAA CACCCCCGAG
    ACTGGGCACA ACCAGCTGTT ACAGCCTCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAT
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTACCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC
    AACCAGCTCT GTATGTGCCC TCCAGCTGAA CCGAAATCAG GGACCAGCAG CACAAATACC
    GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC CAGGCTCAGC CTCCTGCTAT GACTTCCAGT
    AGGAAGGGCA CATTCACAGA TGACCTGCAC AAGTTAGTAG ACAACTGGGC CCGAGATGCC
    ATGAATCTTT CAGGCAGGAG AGGCAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG TGTGTTCCCA TGACCTCAAA CCTGGGTGGC
    TCTACCCCCA TCTCTGCAGC ATCTGCTACC TCTCTAGGTC ACTTCACCAA ATCCATGTGT
    CCCCCACAGC AGTATGGTTT TCCACCTGCC CCATTTGGCA CTCAGTGGAG TGGAACAGGT
    GGTCCAGCAC CGCAGCCACT TGGCCAGTTC CAACCTGTAG GAACAGCATC CTTGCAAAAT
    TTCAACATCA GCAATTTGCA GAAGTCCATC AGCAACCCCC CAGGTTCCAA TCTACGGACC
    ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177039.1
    CDS885..7850
    Translation

    Target ORF information:

    RefSeq Version XM_017321550.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321550.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC CTGTGAATCA GCTGCAGAGT CAGGTTCACC CACAGCTTCT ACAGCCAACA
    ACAGTTCAGT CCATAGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGG TCCACTTCCC
    TCTGGAGATG TTTTGTACCA GGGCTTCCCA TCTCGACTGC CACCACAATA CCCAGGAGAT
    TCAAATATTG CTCCCTCTTC CAACGTGGCT TCTGTTTGCA TCCATTCTAC AGTCCTAGCC
    CCTCCTTCTA TGCCGACAGA AGCATTGGCC ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG
    CCTTATGTGG AATCAACTCC TTTGGTTCCT ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG
    TCCCAACCAG CAGTGAGTTT AACGCAACAA CCCCCAACTA CATCCTCCCA GCAAGCAGTT
    CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT GCTCCTCCAG AACAAACTCC AATAACACAG
    TCACAACCTA CTCAGCCTGT TCCATTGGTT ACCTCTGCAG ACAGTGCACA TTCAGACGTT
    GCTTCTGGTA TGAGTGATGG CAATGAGAAT GCTCCATCAT CCAGCGGGAG GCATGAAGGG
    AGGACAACAA AACGGCATTA TAGAAAATCC GTAAGAAGTC GCTCTCGGCA TGAAAAGACT
    TCTCGCCCAA AACTGAGGAT TTTAAATGTT TCAAATAAAG GAGACCGAGT AGTAGAATGT
    CAATTAGAGA CTCATAATCG GAAGATGGTT ACATTTAAAT TTGACCTAGA TGGTGACAAC
    CCTGAAGAGA TAGCAACAAT TATGGTGAAC AATGACTTTA TTCTAGCAAT AGAGAGAGAG
    TCTTTTGTGG CTCAAGTGCG GGAAATTATT GAAAAGGCTG ATGAAATGTT AAGTGAGGAT
    GTCAGTGTGG AGCCTGAGGG TGACCAGGGA TTGGAGAGTC TGCAAGGAAA GGATGACTAT
    GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA GAGTTCAAGC AACCAATTGC TGTATCTTCC
    ATGCCACAAC AAATAGGTGT TCCTACCAGT TCTTTAACGC AAGTTGTTCA TTCTGCTGGA
    AGACGGTTTA TAGTGAGTCC TGTACCTGAA AGTAGGTTAC GAGAATCAAA AGTTTTCACC
    AGTGACATAT CAGATCCAGT TGTGGCCTCT ACATCTCAGG CCCCTGGAAT GAACTTATCT
    CACTCTGCTT CATCCCTCAG TCTCCAGCAG GCTTTTTCTG AACTGAAACA TGGTCAGATG
    ACTGAAGGAC CCAATACAGC ACCTCCAAAT TTTAATCACA TGGCGGGACC AACATTTTCT
    CCCTTTTTGG CTAGCATTGC TGGTGTCCAA ACTGTAGCAG CATCCACACC GTCAGTATCA
    GTCCCTATAA CAAGCAGTCC TCTTAATGAC ATTTCCACAT CAGTGATGCA GTCTGAGACT
    GCACTGCCCA CTGAAAAAGG CATCGTGGGA GTTACCACTA CCAGCACAGG TGTGGTAGCT
    TCAGGTGGTC TCACCACAAT GTCTGTGTCT GAGTCACCAA CATCTTCCAG TGCGGTTTCA
    AGTTCTACAG TACCTGCAGT TGTCACTGTG TCCACACCAT CCCAGCCAGT TCAGGCTTCC
    ACATCTGGGA GCATTGCTTC AAGTACAGGC AGTTTTCCTC CTGGAACATT TTCTACAACT
    ACAGCTACTA CAATGGGTAG TGTGGTGGCC CCAGATGCTA AGCCTCCAAC TGTATTATTG
    CAACAGGTAG CAAGCAATAC TGCTGGGGTT GCCATAGTGA CATCAGTCTC TACCACCACT
    CCATTCCCAG GCATGGCTTC ACAGCCATCC CTTCCACTTA GTAGCAGCAC CTCTGCTCCT
    ACTCTAGCTG AAACAATGGT GGTTAGTGCA CACTCACTAG ACAAGGCATC TCACAGTAGT
    ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG CCATCCTCTT CCTCCTCCTC TGGAACAGCT
    GTGTCTACTT CTGTTTCTCA GCCTGGTATG GTTCATCCTT TGGTCATCTC ATCAGCTGTA
    GTTTCTACAC CTGGCCTCCC CCAACCAGTA GTACCTACTT CTACACCTTT ATTACCCCAA
    GTACCTAATA TCCCACCCTT GGTACAACCT GTTGTTAATG TGCCTGCTGT ACAGCAAACA
    CTCATTCACA GTCAGCCTCA ACCAGCTCTG CTTCCCAACC AGCCTCACAC ACACTGTCCT
    GAAATGGATG CTGATACCCA ATCCAAAGCT CCTGGGATTG ATGATATAAA GACTCTGGAG
    GAAAAGCTGA GATCTCTCTT TAGTGAACAC AGCTCATCTG GAACTCAGCA TGCTTCTGTC
    TCCCTAGAGA CACCTTTAGT AGTAGAGACG ACTGTCACAC CGGGCATCAC TACCACTGCT
    GTAGCACCAA GTAAACTCAT GACTTCCACT ACAAGTACTT GCTTACCACC AACCAGTTTA
    CCATTAGGAG CAGCTGGCAT GCCAGTTATG CCAGTAGGCA CACCTGGGCA GGTTTCTACC
    CCAGGCACAC ACGCTTCAGC CCCAGTCGGC ACTGCAACAG GAGTAAAACC TGGAACAACT
    CCCCCAAAGC CAACTAAGAC TGTGGTGCCA CCAGTGGGTA CTGAACTTTC AGCCGGTACT
    GTACCCTGTG AGCAGCTGCC ACCTTTTCCT GGACCTTCTC TAATTCAGTC CCAGCAACCA
    CTAGAAGATC TTGATGCTCA GTTGAGAAGA ACACTTAGTC CAGAGACTAT TACAGTGGCA
    CCTGCAGTTG GTCCTTTGTC TACGATGTCT TCAACAACAG TTACAGAAGC AGGGACTCGG
    CTTCAAAAGG ATGGCACAGA AGGTCATGTC ACAGCAACTA GTTCAGGAGC TGGTGTTGTT
    AAGATGGGGC GATTTCAGGT TTCAGTTACA ATGGATGATG CCCAGAAAGA ACGTAAAAAT
    AGGTCAGAAG ATACAAAGTC GGTTCATTTT GAATCTAGCA CATCAGAGTC ATCAGTTCTT
    TCAAGTAGTA GTCCAGAGAG TACCCTGGTA AAGCCAGAGC CTAATGGAAT TAGTATTTCT
    GGCATCTCCT TAGATGTCCC AGACAGCACC CACAAAGCAC CTACCCCAGA AGCAAAGTCA
    GATGCAGGAC AGCCTACCAA GGTGGGACGT TTTCAGGTGA CAACTACAGC CAACAAAGTG
    GGTCGTTTCT CTGTTTCAAG AACTGAGGAC AAGGTCACAG AGCTCAAAAA AGAGGGACCA
    GTGACCTCTC CTCCTTTCAG AGATTCAGAA CAAACTGTTA TTCCTGCTGT GATACCAAAG
    AAGGAGAAGC CTGAGCTGGC AGAACCTTCC CATTTGAATG GGCCATCTTC AGACCTGGAG
    GCTGCCTTTC TAAGTAGGGG TACAGAGGAT GGCTCAGGTA GTCCACACTC TCCCCCTCAC
    CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCATACA TGAGTAGTGA CAATGAGTCC GACATTGAAG ATGAAGACTT AAGGTTAGAA
    CTACGACGAC TACGAGAAAA ACATCTTAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACTAAACTG GGCAAGGTTC CCCCTGCTGT CATTATTCCC
    CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG AGACCCACTA AAAGCAAAGG CAGCAAGTCT
    AGTCGCAGCA GCTCATTGGG CAATAAAAGC CCACAGCTTT CAGGCAACCT GTCTGGTCAG
    AGTGGAACTT CAGTCTTACA CCCCCAACAG ACCCTCCATC CCGCAGGAAA CACCCCCGAG
    ACTGGGCACA ACCAGCTGTT ACAGCCTCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAT
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTACCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC
    AACCAGCTCT GTATGTGCCC TCCAGCTGAA CCGAAATCAG GGACCAGCAG CACAAATACC
    GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC CAGGCTCAGC CTCCTGCTAT GACTTCCAGT
    AGGAAGGGCA CATTCACAGA TGACCTGCAC AAGTTAGTAG ACAACTGGGC CCGAGATGCC
    ATGAATCTTT CAGGCAGGAG AGGCAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG TGTGTTCCCA TGACCTCAAA CCTGGGTGGC
    TCTACCCCCA TCTCTGCAGC ATCTGCTACC TCTCTAGGTC ACTTCACCAA ATCCATGTGT
    CCCCCACAGC AGTATGGTTT TCCACCTGCC CCATTTGGCA CTCAGTGGAG TGGAACAGGT
    GGTCCAGCAC CGCAGCCACT TGGCCAGTTC CAACCTGTAG GAACAGCATC CTTGCAAAAT
    TTCAACATCA GCAATTTGCA GAAGTCCATC AGCAACCCCC CAGGTTCCAA TCTACGGACC
    ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81711
    Clone ID Related Accession (Same CDS sequence) XM_017321552.1
    Accession Version XM_017321552.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGG GCTTCCCATC TCGACTGCCA CCACAATACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTAGCCCC TCCTTCTATG
    CCGACAGAAG CATTGGCCAC CCAAGGTTAC TTTCCTACAG TAGTGCAGCC TTATGTGGAA
    TCAACTCCTT TGGTTCCTAT GGGCAGTGTA GGAGGCCAGG TTCAAGTGTC CCAACCAGCA
    GTGAGTTTAA CGCAACAACC CCCAACTACA TCCTCCCAGC AAGCAGTTCT GGAGAGTACT
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAGCCTGTTC CATTGGTTAC CTCTGCAGAC AGTGCACATT CAGACGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCATCATCC AGCGGGAGGC ATGAAGGGAG GACAACAAAA
    CGGCATTATA GAAAATCCGT AAGAAGTCGC TCTCGGCATG AAAAGACTTC TCGCCCAAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTAGAGACT
    CATAATCGGA AGATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GTGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTG CAAGGAAAGG ATGACTATGG CTTCCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACGCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TACCTGAAAG TAGGTTACGA GAATCAAAAG TTTTCACCAG TGACATATCA
    GATCCAGTTG TGGCCTCTAC ATCTCAGGCC CCTGGAATGA ACTTATCTCA CTCTGCTTCA
    TCCCTCAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACATG GCGGGACCAA CATTTTCTCC CTTTTTGGCT
    AGCATTGCTG GTGTCCAAAC TGTAGCAGCA TCCACACCGT CAGTATCAGT CCCTATAACA
    AGCAGTCCTC TTAATGACAT TTCCACATCA GTGATGCAGT CTGAGACTGC ACTGCCCACT
    GAAAAAGGCA TCGTGGGAGT TACCACTACC AGCACAGGTG TGGTAGCTTC AGGTGGTCTC
    ACCACAATGT CTGTGTCTGA GTCACCAACA TCTTCCAGTG CGGTTTCAAG TTCTACAGTA
    CCTGCAGTTG TCACTGTGTC CACACCATCC CAGCCAGTTC AGGCTTCCAC ATCTGGGAGC
    ATTGCTTCAA GTACAGGCAG TTTTCCTCCT GGAACATTTT CTACAACTAC AGCTACTACA
    ATGGGTAGTG TGGTGGCCCC AGATGCTAAG CCTCCAACTG TATTATTGCA ACAGGTAGCA
    AGCAATACTG CTGGGGTTGC CATAGTGACA TCAGTCTCTA CCACCACTCC ATTCCCAGGC
    ATGGCTTCAC AGCCATCCCT TCCACTTAGT AGCAGCACCT CTGCTCCTAC TCTAGCTGAA
    ACAATGGTGG TTAGTGCACA CTCACTAGAC AAGGCATCTC ACAGTAGTAC AGCAGGATTG
    GGTTTGTCCT TTTGTGCGCC ATCCTCTTCC TCCTCCTCTG GAACAGCTGT GTCTACTTCT
    GTTTCTCAGC CTGGTATGGT TCATCCTTTG GTCATCTCAT CAGCTGTAGT TTCTACACCT
    GGCCTCCCCC AACCAGTAGT ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAATATC
    CCACCCTTGG TACAACCTGT TGTTAATGTG CCTGCTGTAC AGCAAACACT CATTCACAGT
    CAGCCTCAAC CAGCTCTGCT TCCCAACCAG CCTCACACAC ACTGTCCTGA AATGGATGCT
    GATACCCAAT CCAAAGCTCC TGGGATTGAT GATATAAAGA CTCTGGAGGA AAAGCTGAGA
    TCTCTCTTTA GTGAACACAG CTCATCTGGA ACTCAGCATG CTTCTGTCTC CCTAGAGACA
    CCTTTAGTAG TAGAGACGAC TGTCACACCG GGCATCACTA CCACTGCTGT AGCACCAAGT
    AAACTCATGA CTTCCACTAC AAGTACTTGC TTACCACCAA CCAGTTTACC ATTAGGAGCA
    GCTGGCATGC CAGTTATGCC AGTAGGCACA CCTGGGCAGG TTTCTACCCC AGGCACACAC
    GCTTCAGCCC CAGTCGGCAC TGCAACAGGA GTAAAACCTG GAACAACTCC CCCAAAGCCA
    ACTAAGACTG TGGTGCCACC AGTGGGTACT GAACTTTCAG CCGGTACTGT ACCCTGTGAG
    CAGCTGCCAC CTTTTCCTGG ACCTTCTCTA ATTCAGTCCC AGCAACCACT AGAAGATCTT
    GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTA CAGTGGCACC TGCAGTTGGT
    CCTTTGTCTA CGATGTCTTC AACAACAGTT ACAGAAGCAG GGACTCGGCT TCAAAAGGAT
    GGCACAGAAG GTCATGTCAC AGCAACTAGT TCAGGAGCTG GTGTTGTTAA GATGGGGCGA
    TTTCAGGTTT CAGTTACAAT GGATGATGCC CAGAAAGAAC GTAAAAATAG GTCAGAAGAT
    ACAAAGTCGG TTCATTTTGA ATCTAGCACA TCAGAGTCAT CAGTTCTTTC AAGTAGTAGT
    CCAGAGAGTA CCCTGGTAAA GCCAGAGCCT AATGGAATTA GTATTTCTGG CATCTCCTTA
    GATGTCCCAG ACAGCACCCA CAAAGCACCT ACCCCAGAAG CAAAGTCAGA TGCAGGACAG
    CCTACCAAGG TGGGACGTTT TCAGGTGACA ACTACAGCCA ACAAAGTGGG TCGTTTCTCT
    GTTTCAAGAA CTGAGGACAA GGTCACAGAG CTCAAAAAAG AGGGACCAGT GACCTCTCCT
    CCTTTCAGAG ATTCAGAACA AACTGTTATT CCTGCTGTGA TACCAAAGAA GGAGAAGCCT
    GAGCTGGCAG AACCTTCCCA TTTGAATGGG CCATCTTCAG ACCTGGAGGC TGCCTTTCTA
    AGTAGGGGTA CAGAGGATGG CTCAGGTAGT CCACACTCTC CCCCTCACCT GTGTTCAAAG
    AGCCTTCCCG TCCAGAATCT AAGTCAAAGC CTTAGTAATT CATTCAACTC CTCATACATG
    AGTAGTGACA ATGAGTCCGA CATTGAAGAT GAAGACTTAA GGTTAGAACT ACGACGACTA
    CGAGAAAAAC ATCTTAAAGA GATTCAGGAC CTGCAGAGTC GCCAGAAGCA TGAAATTGAA
    TCTTTGTATA CTAAACTGGG CAAGGTTCCC CCTGCTGTCA TTATTCCCCC AGCTGCTCCT
    CTGTCGGGGA GAAGAAGGAG ACCCACTAAA AGCAAAGGCA GCAAGTCTAG TCGCAGCAGC
    TCATTGGGCA ATAAAAGCCC ACAGCTTTCA GGCAACCTGT CTGGTCAGAG TGGAACTTCA
    GTCTTACACC CCCAACAGAC CCTCCATCCC GCAGGAAACA CCCCCGAGAC TGGGCACAAC
    CAGCTGTTAC AGCCTCTTAA GCCATCTCCC TCCAGTGACA ACCTCTATTC AGCCTTTACC
    AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGCTG TGCGAAGTTT
    AACTGTGCAT CTGAGCAGGT GACATTCAAA CCTGGTGGCA GGAGGACCCG ATTTCTGAGT
    ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT
    ATGTGCCCTC CAGCTGAACC GAAATCAGGG ACCAGCAGCA CAAATACCGT TGGAGGAACA
    GTGAGTAGCC AGGCTGCCCA GGCTCAGCCT CCTGCTATGA CTTCCAGTAG GAAGGGCACA
    TTCACAGATG ACCTGCACAA GTTAGTAGAC AACTGGGCCC GAGATGCCAT GAATCTTTCA
    GGCAGGAGAG GCAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC
    TCTGCTCCTG GGCAGCTGTG TGTTCCCATG ACCTCAAACC TGGGTGGCTC TACCCCCATC
    TCTGCAGCAT CTGCTACCTC TCTAGGTCAC TTCACCAAAT CCATGTGTCC CCCACAGCAG
    TATGGTTTTC CACCTGCCCC ATTTGGCACT CAGTGGAGTG GAACAGGTGG TCCAGCACCG
    CAGCCACTTG GCCAGTTCCA ACCTGTAGGA ACAGCATCCT TGCAAAATTT CAACATCAGC
    AATTTGCAGA AGTCCATCAG CAACCCCCCA GGTTCCAATC TACGGACCAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177041.1
    CDS885..7718
    Translation

    Target ORF information:

    RefSeq Version XM_017321552.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321552.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGG GCTTCCCATC TCGACTGCCA CCACAATACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTAGCCCC TCCTTCTATG
    CCGACAGAAG CATTGGCCAC CCAAGGTTAC TTTCCTACAG TAGTGCAGCC TTATGTGGAA
    TCAACTCCTT TGGTTCCTAT GGGCAGTGTA GGAGGCCAGG TTCAAGTGTC CCAACCAGCA
    GTGAGTTTAA CGCAACAACC CCCAACTACA TCCTCCCAGC AAGCAGTTCT GGAGAGTACT
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAGCCTGTTC CATTGGTTAC CTCTGCAGAC AGTGCACATT CAGACGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCATCATCC AGCGGGAGGC ATGAAGGGAG GACAACAAAA
    CGGCATTATA GAAAATCCGT AAGAAGTCGC TCTCGGCATG AAAAGACTTC TCGCCCAAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTAGAGACT
    CATAATCGGA AGATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GTGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTG CAAGGAAAGG ATGACTATGG CTTCCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACGCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TACCTGAAAG TAGGTTACGA GAATCAAAAG TTTTCACCAG TGACATATCA
    GATCCAGTTG TGGCCTCTAC ATCTCAGGCC CCTGGAATGA ACTTATCTCA CTCTGCTTCA
    TCCCTCAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACATG GCGGGACCAA CATTTTCTCC CTTTTTGGCT
    AGCATTGCTG GTGTCCAAAC TGTAGCAGCA TCCACACCGT CAGTATCAGT CCCTATAACA
    AGCAGTCCTC TTAATGACAT TTCCACATCA GTGATGCAGT CTGAGACTGC ACTGCCCACT
    GAAAAAGGCA TCGTGGGAGT TACCACTACC AGCACAGGTG TGGTAGCTTC AGGTGGTCTC
    ACCACAATGT CTGTGTCTGA GTCACCAACA TCTTCCAGTG CGGTTTCAAG TTCTACAGTA
    CCTGCAGTTG TCACTGTGTC CACACCATCC CAGCCAGTTC AGGCTTCCAC ATCTGGGAGC
    ATTGCTTCAA GTACAGGCAG TTTTCCTCCT GGAACATTTT CTACAACTAC AGCTACTACA
    ATGGGTAGTG TGGTGGCCCC AGATGCTAAG CCTCCAACTG TATTATTGCA ACAGGTAGCA
    AGCAATACTG CTGGGGTTGC CATAGTGACA TCAGTCTCTA CCACCACTCC ATTCCCAGGC
    ATGGCTTCAC AGCCATCCCT TCCACTTAGT AGCAGCACCT CTGCTCCTAC TCTAGCTGAA
    ACAATGGTGG TTAGTGCACA CTCACTAGAC AAGGCATCTC ACAGTAGTAC AGCAGGATTG
    GGTTTGTCCT TTTGTGCGCC ATCCTCTTCC TCCTCCTCTG GAACAGCTGT GTCTACTTCT
    GTTTCTCAGC CTGGTATGGT TCATCCTTTG GTCATCTCAT CAGCTGTAGT TTCTACACCT
    GGCCTCCCCC AACCAGTAGT ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAATATC
    CCACCCTTGG TACAACCTGT TGTTAATGTG CCTGCTGTAC AGCAAACACT CATTCACAGT
    CAGCCTCAAC CAGCTCTGCT TCCCAACCAG CCTCACACAC ACTGTCCTGA AATGGATGCT
    GATACCCAAT CCAAAGCTCC TGGGATTGAT GATATAAAGA CTCTGGAGGA AAAGCTGAGA
    TCTCTCTTTA GTGAACACAG CTCATCTGGA ACTCAGCATG CTTCTGTCTC CCTAGAGACA
    CCTTTAGTAG TAGAGACGAC TGTCACACCG GGCATCACTA CCACTGCTGT AGCACCAAGT
    AAACTCATGA CTTCCACTAC AAGTACTTGC TTACCACCAA CCAGTTTACC ATTAGGAGCA
    GCTGGCATGC CAGTTATGCC AGTAGGCACA CCTGGGCAGG TTTCTACCCC AGGCACACAC
    GCTTCAGCCC CAGTCGGCAC TGCAACAGGA GTAAAACCTG GAACAACTCC CCCAAAGCCA
    ACTAAGACTG TGGTGCCACC AGTGGGTACT GAACTTTCAG CCGGTACTGT ACCCTGTGAG
    CAGCTGCCAC CTTTTCCTGG ACCTTCTCTA ATTCAGTCCC AGCAACCACT AGAAGATCTT
    GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTA CAGTGGCACC TGCAGTTGGT
    CCTTTGTCTA CGATGTCTTC AACAACAGTT ACAGAAGCAG GGACTCGGCT TCAAAAGGAT
    GGCACAGAAG GTCATGTCAC AGCAACTAGT TCAGGAGCTG GTGTTGTTAA GATGGGGCGA
    TTTCAGGTTT CAGTTACAAT GGATGATGCC CAGAAAGAAC GTAAAAATAG GTCAGAAGAT
    ACAAAGTCGG TTCATTTTGA ATCTAGCACA TCAGAGTCAT CAGTTCTTTC AAGTAGTAGT
    CCAGAGAGTA CCCTGGTAAA GCCAGAGCCT AATGGAATTA GTATTTCTGG CATCTCCTTA
    GATGTCCCAG ACAGCACCCA CAAAGCACCT ACCCCAGAAG CAAAGTCAGA TGCAGGACAG
    CCTACCAAGG TGGGACGTTT TCAGGTGACA ACTACAGCCA ACAAAGTGGG TCGTTTCTCT
    GTTTCAAGAA CTGAGGACAA GGTCACAGAG CTCAAAAAAG AGGGACCAGT GACCTCTCCT
    CCTTTCAGAG ATTCAGAACA AACTGTTATT CCTGCTGTGA TACCAAAGAA GGAGAAGCCT
    GAGCTGGCAG AACCTTCCCA TTTGAATGGG CCATCTTCAG ACCTGGAGGC TGCCTTTCTA
    AGTAGGGGTA CAGAGGATGG CTCAGGTAGT CCACACTCTC CCCCTCACCT GTGTTCAAAG
    AGCCTTCCCG TCCAGAATCT AAGTCAAAGC CTTAGTAATT CATTCAACTC CTCATACATG
    AGTAGTGACA ATGAGTCCGA CATTGAAGAT GAAGACTTAA GGTTAGAACT ACGACGACTA
    CGAGAAAAAC ATCTTAAAGA GATTCAGGAC CTGCAGAGTC GCCAGAAGCA TGAAATTGAA
    TCTTTGTATA CTAAACTGGG CAAGGTTCCC CCTGCTGTCA TTATTCCCCC AGCTGCTCCT
    CTGTCGGGGA GAAGAAGGAG ACCCACTAAA AGCAAAGGCA GCAAGTCTAG TCGCAGCAGC
    TCATTGGGCA ATAAAAGCCC ACAGCTTTCA GGCAACCTGT CTGGTCAGAG TGGAACTTCA
    GTCTTACACC CCCAACAGAC CCTCCATCCC GCAGGAAACA CCCCCGAGAC TGGGCACAAC
    CAGCTGTTAC AGCCTCTTAA GCCATCTCCC TCCAGTGACA ACCTCTATTC AGCCTTTACC
    AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGCTG TGCGAAGTTT
    AACTGTGCAT CTGAGCAGGT GACATTCAAA CCTGGTGGCA GGAGGACCCG ATTTCTGAGT
    ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT
    ATGTGCCCTC CAGCTGAACC GAAATCAGGG ACCAGCAGCA CAAATACCGT TGGAGGAACA
    GTGAGTAGCC AGGCTGCCCA GGCTCAGCCT CCTGCTATGA CTTCCAGTAG GAAGGGCACA
    TTCACAGATG ACCTGCACAA GTTAGTAGAC AACTGGGCCC GAGATGCCAT GAATCTTTCA
    GGCAGGAGAG GCAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC
    TCTGCTCCTG GGCAGCTGTG TGTTCCCATG ACCTCAAACC TGGGTGGCTC TACCCCCATC
    TCTGCAGCAT CTGCTACCTC TCTAGGTCAC TTCACCAAAT CCATGTGTCC CCCACAGCAG
    TATGGTTTTC CACCTGCCCC ATTTGGCACT CAGTGGAGTG GAACAGGTGG TCCAGCACCG
    CAGCCACTTG GCCAGTTCCA ACCTGTAGGA ACAGCATCCT TGCAAAATTT CAACATCAGC
    AATTTGCAGA AGTCCATCAG CAACCCCCCA GGTTCCAATC TACGGACCAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81712
    Clone ID Related Accession (Same CDS sequence) XM_017321553.2 , XM_017321553.1
    Accession Version XM_017321553.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6672bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGG GCTTCCCATC TCGACTGCCA CCACAATACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTAGCCCC TCCTTCTATG
    CCGACAGAAG CATTGGCCAC CCAAGGTTAC TTTCCTACAG TAGTGCAGCC TTATGTGGAA
    TCAACTCCTT TGGTTCCTAT GGGCAGTGTA GGAGGCCAGG TTCAAGTGTC CCAACCAGCA
    GTGAGTTTAA CGCAACAACC CCCAACTACA TCCTCCCAGC AAGCAGTTCT GGAGAGTACT
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAGCCTGTTC CATTGGTTAC CTCTGCAGAC AGTGCACATT CAGACGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCATCATCC AGCGGGAGGC ATGAAGGGAG GACAACAAAA
    CGGCATTATA GAAAATCCGT AAGAAGTCGC TCTCGGCATG AAAAGACTTC TCGCCCAAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTAGAGACT
    CATAATCGGA AGATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GTGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTG CAAGGAAAGG ATGACTATGG CTTCCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACGCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TACCTGAAAG TAGGTTACGA GAATCAAAAG TTTTCACCAG TGACATATCA
    GATCCAGTTG TGGCCTCTAC ATCTCAGGCC CCTGGAATGA ACTTATCTCA CTCTGCTTCA
    TCCCTCAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACATG GCGGGACCAA CATTTTCTCC CTTTTTGGCT
    AGCATTGCTG GTGTCCAAAC TGTAGCAGCA TCCACACCGT CAGTATCAGT CCCTATAACA
    AGCAGTCCTC TTAATGACAT TTCCACATCA GTGATGCAGT CTGAGACTGC ACTGCCCACT
    GAAAAAGGCA TCGTGGGAGT TACCACTACC AGCACAGGTG TGGTAGCTTC AGGTGGTCTC
    ACCACAATGT CTGTGTCTGA GTCACCAACA TCTTCCAGTG CGGTTTCAAG TTCTACAGTA
    CCTGCAGTTG TCACTGTGTC CACACCATCC CAGCCAGTTC AGGCTTCCAC ATCTGGGAGC
    ATTGCTTCAA GTACAGGCAG TTTTCCTCCT GGAACATTTT CTACAACTAC AGCTACTACA
    ATGGGTAGTG TGGTGGCCCC AGATGCTAAG CCTCCAACTG TATTATTGCA ACAGGTAGCA
    AGCAATACTG CTGGGGTTGC CATAGTGACA TCAGTCTCTA CCACCACTCC ATTCCCAGGC
    ATGGCTTCAC AGCCATCCCT TCCACTTAGT AGCAGCACCT CTGCTCCTAC TCTAGCTGAA
    ACAATGGTGG TTAGTGCACA CTCACTAGAC AAGGCATCTC ACAGTAGTAC AGCAGGATTG
    GGTTTGTCCT TTTGTGCGCC ATCCTCTTCC TCCTCCTCTG GAACAGCTGT GTCTACTTCT
    GTTTCTCAGC CTGGTATGGT TCATCCTTTG GTCATCTCAT CAGCTGTAGT TTCTACACCT
    GGCCTCCCCC AACCAGTAGT ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAATATC
    CCACCCTTGG TACAACCTGT TGTTAATGTG CCTGCTGTAC AGCAAACACT CATTCACAGT
    CAGCCTCAAC CAGCTCTGCT TCCCAACCAG CCTCACACAC ACTGTCCTGA AATGGATGCT
    GATACCCAAT CCAAAGCTCC TGGGATTGAT GATATAAAGA CTCTGGAGGA AAAGCTGAGA
    TCTCTCTTTA GTGAACACAG CTCATCTGGA ACTCAGCATG CTTCTGTCTC CCTAGAGACA
    CCTTTAGTAG TAGAGACGAC TGTCACACCG GGCATCACTA CCACTGCTGT AGCACCAAGT
    AAACTCATGA CTTCCACTAC AAGTACTTGC TTACCACCAA CCAGTTTACC ATTAGGAGCA
    GCTGGCATGC CAGTTATGCC AGTAGGCACA CCTGGGCAGG TTTCTACCCC AGGCACACAC
    GCTTCAGCCC CAGTCGGCAC TGCAACAGGA GTAAAACCTG GAACAACTCC CCCAAAGCCA
    ACTAAGACTG TGGTGCCACC AGTGGGTACT GAACTTTCAG CCGGTACTGT ACCCTGTGAG
    CAGCTGCCAC CTTTTCCTGG ACCTTCTCTA ATTCAGTCCC AGCAACCACT AGAAGATCTT
    GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTA CAGTGGCACC TGCAGTTGGT
    CCTTTGTCTA CGATGTCTTC AACAACAGTT ACAGAAGCAG GGACTCGGCT TCAAAAGGAT
    GGCACAGAAG GTCATGTCAC AGCAACTAGT TCAGGAGCTG GTGTTGTTAA GATGGGGCGA
    TTTCAGGTTT CAGTTACAAT GGATGATGCC CAGAAAGAAC GTAAAAATAG GTCAGAAGAT
    ACAAAGTCGG TTCATTTTGA ATCTAGCACA TCAGAGTCAT CAGTTCTTTC AAGTAGTAGT
    CCAGAGAGTA CCCTGGTAAA GCCAGAGCCT AATGGAATTA GTATTTCTGG CATCTCCTTA
    GATGTCCCAG ACAGCACCCA CAAAGCACCT ACCCCAGAAG CAAAGTCAGA TGCAGGACAG
    CCTACCAAGG TGGGACGTTT TCAGGTGACA ACTACAGCCA ACAAAGTGGG TCGTTTCTCT
    GTTTCAAGAA CTGAGGACAA GGTCACAGAG CTCAAAAAAG AGGGACCAGT GACCTCTCCT
    CCTTTCAGAG ATTCAGAACA AACTGTTATT CCTGCTGTGA TACCAAAGAA GGAGAAGCCT
    GAGCTGGCAG AACCTTCCCA TTTGAATGGG CCATCTTCAG ACCTGGAGGC TGCCTTTCTA
    AGTAGGGGTA CAGAGGATGG CTCAGGTAGT CCACACTCTC CCCCTCACCT GTGTTCAAAG
    AGCCTTCCCG TCCAGAATCT AAGTCAAAGC CTTAGTAATT CATTCAACTC CTCATACATG
    AGTAGTGACA ATGAGTCCGA CATTGAAGAT GAAGACTTAA GGTTAGAACT ACGACGACTA
    CGAGAAAAAC ATCTTAAAGA GATTCAGGAC CTGCAGAGTC GCCAGAAGCA TGAAATTGAA
    TCTTTGTATA CTAAACTGGG CAAGGTTCCC CCTGCTGTCA TTATTCCCCC AGCTGCTCCT
    CTGTCGGGGA GAAGAAGGAG ACCCACTAAA AGCAAAGGCA GCAAGTCTAG TCGCAGCAGC
    TCATTGGGCA ATAAAAGCCC ACAGCTTTCA GGCAACCTGT CTGGTCAGAG TGGAACTTCA
    GTCTTACACC CCCAACAGAC CCTCCATCCC GCAGGAAACA CCCCCGAGAC TGGGCACAAC
    CAGCTGTTAC AGCCTCTTAA GCCATCTCCC TCCAGTGACA ACCTCTATTC AGCCTTTACC
    AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGGAC CAGCAGCACA
    AATACCGTTG GAGGAACAGT GAGTAGCCAG GCTGCCCAGG CTCAGCCTCC TGCTATGACT
    TCCAGTAGGA AGGGCACATT CACAGATGAC CTGCACAAGT TAGTAGACAA CTGGGCCCGA
    GATGCCATGA ATCTTTCAGG CAGGAGAGGC AGCAAAGGAC ACATGAATTA TGAGGGCCCT
    GGAATGGCAA GGAAGTTCTC TGCTCCTGGG CAGCTGTGTG TTCCCATGAC CTCAAACCTG
    GGTGGCTCTA CCCCCATCTC TGCAGCATCT GCTACCTCTC TAGGTCACTT CACCAAATCC
    ATGTGTCCCC CACAGCAGTA TGGTTTTCCA CCTGCCCCAT TTGGCACTCA GTGGAGTGGA
    ACAGGTGGTC CAGCACCGCA GCCACTTGGC CAGTTCCAAC CTGTAGGAAC AGCATCCTTG
    CAAAATTTCA ACATCAGCAA TTTGCAGAAG TCCATCAGCA ACCCCCCAGG TTCCAATCTA
    CGGACCACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177042.1
    CDS885..7556
    Translation

    Target ORF information:

    RefSeq Version XM_017321553.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321553.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGG GCTTCCCATC TCGACTGCCA CCACAATACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTAGCCCC TCCTTCTATG
    CCGACAGAAG CATTGGCCAC CCAAGGTTAC TTTCCTACAG TAGTGCAGCC TTATGTGGAA
    TCAACTCCTT TGGTTCCTAT GGGCAGTGTA GGAGGCCAGG TTCAAGTGTC CCAACCAGCA
    GTGAGTTTAA CGCAACAACC CCCAACTACA TCCTCCCAGC AAGCAGTTCT GGAGAGTACT
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAGCCTGTTC CATTGGTTAC CTCTGCAGAC AGTGCACATT CAGACGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCATCATCC AGCGGGAGGC ATGAAGGGAG GACAACAAAA
    CGGCATTATA GAAAATCCGT AAGAAGTCGC TCTCGGCATG AAAAGACTTC TCGCCCAAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTAGAGACT
    CATAATCGGA AGATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GTGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTG CAAGGAAAGG ATGACTATGG CTTCCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACGCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TACCTGAAAG TAGGTTACGA GAATCAAAAG TTTTCACCAG TGACATATCA
    GATCCAGTTG TGGCCTCTAC ATCTCAGGCC CCTGGAATGA ACTTATCTCA CTCTGCTTCA
    TCCCTCAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACATG GCGGGACCAA CATTTTCTCC CTTTTTGGCT
    AGCATTGCTG GTGTCCAAAC TGTAGCAGCA TCCACACCGT CAGTATCAGT CCCTATAACA
    AGCAGTCCTC TTAATGACAT TTCCACATCA GTGATGCAGT CTGAGACTGC ACTGCCCACT
    GAAAAAGGCA TCGTGGGAGT TACCACTACC AGCACAGGTG TGGTAGCTTC AGGTGGTCTC
    ACCACAATGT CTGTGTCTGA GTCACCAACA TCTTCCAGTG CGGTTTCAAG TTCTACAGTA
    CCTGCAGTTG TCACTGTGTC CACACCATCC CAGCCAGTTC AGGCTTCCAC ATCTGGGAGC
    ATTGCTTCAA GTACAGGCAG TTTTCCTCCT GGAACATTTT CTACAACTAC AGCTACTACA
    ATGGGTAGTG TGGTGGCCCC AGATGCTAAG CCTCCAACTG TATTATTGCA ACAGGTAGCA
    AGCAATACTG CTGGGGTTGC CATAGTGACA TCAGTCTCTA CCACCACTCC ATTCCCAGGC
    ATGGCTTCAC AGCCATCCCT TCCACTTAGT AGCAGCACCT CTGCTCCTAC TCTAGCTGAA
    ACAATGGTGG TTAGTGCACA CTCACTAGAC AAGGCATCTC ACAGTAGTAC AGCAGGATTG
    GGTTTGTCCT TTTGTGCGCC ATCCTCTTCC TCCTCCTCTG GAACAGCTGT GTCTACTTCT
    GTTTCTCAGC CTGGTATGGT TCATCCTTTG GTCATCTCAT CAGCTGTAGT TTCTACACCT
    GGCCTCCCCC AACCAGTAGT ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAATATC
    CCACCCTTGG TACAACCTGT TGTTAATGTG CCTGCTGTAC AGCAAACACT CATTCACAGT
    CAGCCTCAAC CAGCTCTGCT TCCCAACCAG CCTCACACAC ACTGTCCTGA AATGGATGCT
    GATACCCAAT CCAAAGCTCC TGGGATTGAT GATATAAAGA CTCTGGAGGA AAAGCTGAGA
    TCTCTCTTTA GTGAACACAG CTCATCTGGA ACTCAGCATG CTTCTGTCTC CCTAGAGACA
    CCTTTAGTAG TAGAGACGAC TGTCACACCG GGCATCACTA CCACTGCTGT AGCACCAAGT
    AAACTCATGA CTTCCACTAC AAGTACTTGC TTACCACCAA CCAGTTTACC ATTAGGAGCA
    GCTGGCATGC CAGTTATGCC AGTAGGCACA CCTGGGCAGG TTTCTACCCC AGGCACACAC
    GCTTCAGCCC CAGTCGGCAC TGCAACAGGA GTAAAACCTG GAACAACTCC CCCAAAGCCA
    ACTAAGACTG TGGTGCCACC AGTGGGTACT GAACTTTCAG CCGGTACTGT ACCCTGTGAG
    CAGCTGCCAC CTTTTCCTGG ACCTTCTCTA ATTCAGTCCC AGCAACCACT AGAAGATCTT
    GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTA CAGTGGCACC TGCAGTTGGT
    CCTTTGTCTA CGATGTCTTC AACAACAGTT ACAGAAGCAG GGACTCGGCT TCAAAAGGAT
    GGCACAGAAG GTCATGTCAC AGCAACTAGT TCAGGAGCTG GTGTTGTTAA GATGGGGCGA
    TTTCAGGTTT CAGTTACAAT GGATGATGCC CAGAAAGAAC GTAAAAATAG GTCAGAAGAT
    ACAAAGTCGG TTCATTTTGA ATCTAGCACA TCAGAGTCAT CAGTTCTTTC AAGTAGTAGT
    CCAGAGAGTA CCCTGGTAAA GCCAGAGCCT AATGGAATTA GTATTTCTGG CATCTCCTTA
    GATGTCCCAG ACAGCACCCA CAAAGCACCT ACCCCAGAAG CAAAGTCAGA TGCAGGACAG
    CCTACCAAGG TGGGACGTTT TCAGGTGACA ACTACAGCCA ACAAAGTGGG TCGTTTCTCT
    GTTTCAAGAA CTGAGGACAA GGTCACAGAG CTCAAAAAAG AGGGACCAGT GACCTCTCCT
    CCTTTCAGAG ATTCAGAACA AACTGTTATT CCTGCTGTGA TACCAAAGAA GGAGAAGCCT
    GAGCTGGCAG AACCTTCCCA TTTGAATGGG CCATCTTCAG ACCTGGAGGC TGCCTTTCTA
    AGTAGGGGTA CAGAGGATGG CTCAGGTAGT CCACACTCTC CCCCTCACCT GTGTTCAAAG
    AGCCTTCCCG TCCAGAATCT AAGTCAAAGC CTTAGTAATT CATTCAACTC CTCATACATG
    AGTAGTGACA ATGAGTCCGA CATTGAAGAT GAAGACTTAA GGTTAGAACT ACGACGACTA
    CGAGAAAAAC ATCTTAAAGA GATTCAGGAC CTGCAGAGTC GCCAGAAGCA TGAAATTGAA
    TCTTTGTATA CTAAACTGGG CAAGGTTCCC CCTGCTGTCA TTATTCCCCC AGCTGCTCCT
    CTGTCGGGGA GAAGAAGGAG ACCCACTAAA AGCAAAGGCA GCAAGTCTAG TCGCAGCAGC
    TCATTGGGCA ATAAAAGCCC ACAGCTTTCA GGCAACCTGT CTGGTCAGAG TGGAACTTCA
    GTCTTACACC CCCAACAGAC CCTCCATCCC GCAGGAAACA CCCCCGAGAC TGGGCACAAC
    CAGCTGTTAC AGCCTCTTAA GCCATCTCCC TCCAGTGACA ACCTCTATTC AGCCTTTACC
    AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGGAC CAGCAGCACA
    AATACCGTTG GAGGAACAGT GAGTAGCCAG GCTGCCCAGG CTCAGCCTCC TGCTATGACT
    TCCAGTAGGA AGGGCACATT CACAGATGAC CTGCACAAGT TAGTAGACAA CTGGGCCCGA
    GATGCCATGA ATCTTTCAGG CAGGAGAGGC AGCAAAGGAC ACATGAATTA TGAGGGCCCT
    GGAATGGCAA GGAAGTTCTC TGCTCCTGGG CAGCTGTGTG TTCCCATGAC CTCAAACCTG
    GGTGGCTCTA CCCCCATCTC TGCAGCATCT GCTACCTCTC TAGGTCACTT CACCAAATCC
    ATGTGTCCCC CACAGCAGTA TGGTTTTCCA CCTGCCCCAT TTGGCACTCA GTGGAGTGGA
    ACAGGTGGTC CAGCACCGCA GCCACTTGGC CAGTTCCAAC CTGTAGGAAC AGCATCCTTG
    CAAAATTTCA ACATCAGCAA TTTGCAGAAG TCCATCAGCA ACCCCCCAGG TTCCAATCTA
    CGGACCACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81712
    Clone ID Related Accession (Same CDS sequence) XM_017321553.2 , XM_017321553.1
    Accession Version XM_017321553.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6672bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGG GCTTCCCATC TCGACTGCCA CCACAATACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTAGCCCC TCCTTCTATG
    CCGACAGAAG CATTGGCCAC CCAAGGTTAC TTTCCTACAG TAGTGCAGCC TTATGTGGAA
    TCAACTCCTT TGGTTCCTAT GGGCAGTGTA GGAGGCCAGG TTCAAGTGTC CCAACCAGCA
    GTGAGTTTAA CGCAACAACC CCCAACTACA TCCTCCCAGC AAGCAGTTCT GGAGAGTACT
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAGCCTGTTC CATTGGTTAC CTCTGCAGAC AGTGCACATT CAGACGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCATCATCC AGCGGGAGGC ATGAAGGGAG GACAACAAAA
    CGGCATTATA GAAAATCCGT AAGAAGTCGC TCTCGGCATG AAAAGACTTC TCGCCCAAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTAGAGACT
    CATAATCGGA AGATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GTGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTG CAAGGAAAGG ATGACTATGG CTTCCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACGCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TACCTGAAAG TAGGTTACGA GAATCAAAAG TTTTCACCAG TGACATATCA
    GATCCAGTTG TGGCCTCTAC ATCTCAGGCC CCTGGAATGA ACTTATCTCA CTCTGCTTCA
    TCCCTCAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACATG GCGGGACCAA CATTTTCTCC CTTTTTGGCT
    AGCATTGCTG GTGTCCAAAC TGTAGCAGCA TCCACACCGT CAGTATCAGT CCCTATAACA
    AGCAGTCCTC TTAATGACAT TTCCACATCA GTGATGCAGT CTGAGACTGC ACTGCCCACT
    GAAAAAGGCA TCGTGGGAGT TACCACTACC AGCACAGGTG TGGTAGCTTC AGGTGGTCTC
    ACCACAATGT CTGTGTCTGA GTCACCAACA TCTTCCAGTG CGGTTTCAAG TTCTACAGTA
    CCTGCAGTTG TCACTGTGTC CACACCATCC CAGCCAGTTC AGGCTTCCAC ATCTGGGAGC
    ATTGCTTCAA GTACAGGCAG TTTTCCTCCT GGAACATTTT CTACAACTAC AGCTACTACA
    ATGGGTAGTG TGGTGGCCCC AGATGCTAAG CCTCCAACTG TATTATTGCA ACAGGTAGCA
    AGCAATACTG CTGGGGTTGC CATAGTGACA TCAGTCTCTA CCACCACTCC ATTCCCAGGC
    ATGGCTTCAC AGCCATCCCT TCCACTTAGT AGCAGCACCT CTGCTCCTAC TCTAGCTGAA
    ACAATGGTGG TTAGTGCACA CTCACTAGAC AAGGCATCTC ACAGTAGTAC AGCAGGATTG
    GGTTTGTCCT TTTGTGCGCC ATCCTCTTCC TCCTCCTCTG GAACAGCTGT GTCTACTTCT
    GTTTCTCAGC CTGGTATGGT TCATCCTTTG GTCATCTCAT CAGCTGTAGT TTCTACACCT
    GGCCTCCCCC AACCAGTAGT ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAATATC
    CCACCCTTGG TACAACCTGT TGTTAATGTG CCTGCTGTAC AGCAAACACT CATTCACAGT
    CAGCCTCAAC CAGCTCTGCT TCCCAACCAG CCTCACACAC ACTGTCCTGA AATGGATGCT
    GATACCCAAT CCAAAGCTCC TGGGATTGAT GATATAAAGA CTCTGGAGGA AAAGCTGAGA
    TCTCTCTTTA GTGAACACAG CTCATCTGGA ACTCAGCATG CTTCTGTCTC CCTAGAGACA
    CCTTTAGTAG TAGAGACGAC TGTCACACCG GGCATCACTA CCACTGCTGT AGCACCAAGT
    AAACTCATGA CTTCCACTAC AAGTACTTGC TTACCACCAA CCAGTTTACC ATTAGGAGCA
    GCTGGCATGC CAGTTATGCC AGTAGGCACA CCTGGGCAGG TTTCTACCCC AGGCACACAC
    GCTTCAGCCC CAGTCGGCAC TGCAACAGGA GTAAAACCTG GAACAACTCC CCCAAAGCCA
    ACTAAGACTG TGGTGCCACC AGTGGGTACT GAACTTTCAG CCGGTACTGT ACCCTGTGAG
    CAGCTGCCAC CTTTTCCTGG ACCTTCTCTA ATTCAGTCCC AGCAACCACT AGAAGATCTT
    GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTA CAGTGGCACC TGCAGTTGGT
    CCTTTGTCTA CGATGTCTTC AACAACAGTT ACAGAAGCAG GGACTCGGCT TCAAAAGGAT
    GGCACAGAAG GTCATGTCAC AGCAACTAGT TCAGGAGCTG GTGTTGTTAA GATGGGGCGA
    TTTCAGGTTT CAGTTACAAT GGATGATGCC CAGAAAGAAC GTAAAAATAG GTCAGAAGAT
    ACAAAGTCGG TTCATTTTGA ATCTAGCACA TCAGAGTCAT CAGTTCTTTC AAGTAGTAGT
    CCAGAGAGTA CCCTGGTAAA GCCAGAGCCT AATGGAATTA GTATTTCTGG CATCTCCTTA
    GATGTCCCAG ACAGCACCCA CAAAGCACCT ACCCCAGAAG CAAAGTCAGA TGCAGGACAG
    CCTACCAAGG TGGGACGTTT TCAGGTGACA ACTACAGCCA ACAAAGTGGG TCGTTTCTCT
    GTTTCAAGAA CTGAGGACAA GGTCACAGAG CTCAAAAAAG AGGGACCAGT GACCTCTCCT
    CCTTTCAGAG ATTCAGAACA AACTGTTATT CCTGCTGTGA TACCAAAGAA GGAGAAGCCT
    GAGCTGGCAG AACCTTCCCA TTTGAATGGG CCATCTTCAG ACCTGGAGGC TGCCTTTCTA
    AGTAGGGGTA CAGAGGATGG CTCAGGTAGT CCACACTCTC CCCCTCACCT GTGTTCAAAG
    AGCCTTCCCG TCCAGAATCT AAGTCAAAGC CTTAGTAATT CATTCAACTC CTCATACATG
    AGTAGTGACA ATGAGTCCGA CATTGAAGAT GAAGACTTAA GGTTAGAACT ACGACGACTA
    CGAGAAAAAC ATCTTAAAGA GATTCAGGAC CTGCAGAGTC GCCAGAAGCA TGAAATTGAA
    TCTTTGTATA CTAAACTGGG CAAGGTTCCC CCTGCTGTCA TTATTCCCCC AGCTGCTCCT
    CTGTCGGGGA GAAGAAGGAG ACCCACTAAA AGCAAAGGCA GCAAGTCTAG TCGCAGCAGC
    TCATTGGGCA ATAAAAGCCC ACAGCTTTCA GGCAACCTGT CTGGTCAGAG TGGAACTTCA
    GTCTTACACC CCCAACAGAC CCTCCATCCC GCAGGAAACA CCCCCGAGAC TGGGCACAAC
    CAGCTGTTAC AGCCTCTTAA GCCATCTCCC TCCAGTGACA ACCTCTATTC AGCCTTTACC
    AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGGAC CAGCAGCACA
    AATACCGTTG GAGGAACAGT GAGTAGCCAG GCTGCCCAGG CTCAGCCTCC TGCTATGACT
    TCCAGTAGGA AGGGCACATT CACAGATGAC CTGCACAAGT TAGTAGACAA CTGGGCCCGA
    GATGCCATGA ATCTTTCAGG CAGGAGAGGC AGCAAAGGAC ACATGAATTA TGAGGGCCCT
    GGAATGGCAA GGAAGTTCTC TGCTCCTGGG CAGCTGTGTG TTCCCATGAC CTCAAACCTG
    GGTGGCTCTA CCCCCATCTC TGCAGCATCT GCTACCTCTC TAGGTCACTT CACCAAATCC
    ATGTGTCCCC CACAGCAGTA TGGTTTTCCA CCTGCCCCAT TTGGCACTCA GTGGAGTGGA
    ACAGGTGGTC CAGCACCGCA GCCACTTGGC CAGTTCCAAC CTGTAGGAAC AGCATCCTTG
    CAAAATTTCA ACATCAGCAA TTTGCAGAAG TCCATCAGCA ACCCCCCAGG TTCCAATCTA
    CGGACCACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177042.1
    CDS885..7556
    Translation

    Target ORF information:

    RefSeq Version XM_017321553.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321553.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGG GCTTCCCATC TCGACTGCCA CCACAATACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTAGCCCC TCCTTCTATG
    CCGACAGAAG CATTGGCCAC CCAAGGTTAC TTTCCTACAG TAGTGCAGCC TTATGTGGAA
    TCAACTCCTT TGGTTCCTAT GGGCAGTGTA GGAGGCCAGG TTCAAGTGTC CCAACCAGCA
    GTGAGTTTAA CGCAACAACC CCCAACTACA TCCTCCCAGC AAGCAGTTCT GGAGAGTACT
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAGCCTGTTC CATTGGTTAC CTCTGCAGAC AGTGCACATT CAGACGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCATCATCC AGCGGGAGGC ATGAAGGGAG GACAACAAAA
    CGGCATTATA GAAAATCCGT AAGAAGTCGC TCTCGGCATG AAAAGACTTC TCGCCCAAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTAGAGACT
    CATAATCGGA AGATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GTGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTG CAAGGAAAGG ATGACTATGG CTTCCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACGCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TACCTGAAAG TAGGTTACGA GAATCAAAAG TTTTCACCAG TGACATATCA
    GATCCAGTTG TGGCCTCTAC ATCTCAGGCC CCTGGAATGA ACTTATCTCA CTCTGCTTCA
    TCCCTCAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACATG GCGGGACCAA CATTTTCTCC CTTTTTGGCT
    AGCATTGCTG GTGTCCAAAC TGTAGCAGCA TCCACACCGT CAGTATCAGT CCCTATAACA
    AGCAGTCCTC TTAATGACAT TTCCACATCA GTGATGCAGT CTGAGACTGC ACTGCCCACT
    GAAAAAGGCA TCGTGGGAGT TACCACTACC AGCACAGGTG TGGTAGCTTC AGGTGGTCTC
    ACCACAATGT CTGTGTCTGA GTCACCAACA TCTTCCAGTG CGGTTTCAAG TTCTACAGTA
    CCTGCAGTTG TCACTGTGTC CACACCATCC CAGCCAGTTC AGGCTTCCAC ATCTGGGAGC
    ATTGCTTCAA GTACAGGCAG TTTTCCTCCT GGAACATTTT CTACAACTAC AGCTACTACA
    ATGGGTAGTG TGGTGGCCCC AGATGCTAAG CCTCCAACTG TATTATTGCA ACAGGTAGCA
    AGCAATACTG CTGGGGTTGC CATAGTGACA TCAGTCTCTA CCACCACTCC ATTCCCAGGC
    ATGGCTTCAC AGCCATCCCT TCCACTTAGT AGCAGCACCT CTGCTCCTAC TCTAGCTGAA
    ACAATGGTGG TTAGTGCACA CTCACTAGAC AAGGCATCTC ACAGTAGTAC AGCAGGATTG
    GGTTTGTCCT TTTGTGCGCC ATCCTCTTCC TCCTCCTCTG GAACAGCTGT GTCTACTTCT
    GTTTCTCAGC CTGGTATGGT TCATCCTTTG GTCATCTCAT CAGCTGTAGT TTCTACACCT
    GGCCTCCCCC AACCAGTAGT ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAATATC
    CCACCCTTGG TACAACCTGT TGTTAATGTG CCTGCTGTAC AGCAAACACT CATTCACAGT
    CAGCCTCAAC CAGCTCTGCT TCCCAACCAG CCTCACACAC ACTGTCCTGA AATGGATGCT
    GATACCCAAT CCAAAGCTCC TGGGATTGAT GATATAAAGA CTCTGGAGGA AAAGCTGAGA
    TCTCTCTTTA GTGAACACAG CTCATCTGGA ACTCAGCATG CTTCTGTCTC CCTAGAGACA
    CCTTTAGTAG TAGAGACGAC TGTCACACCG GGCATCACTA CCACTGCTGT AGCACCAAGT
    AAACTCATGA CTTCCACTAC AAGTACTTGC TTACCACCAA CCAGTTTACC ATTAGGAGCA
    GCTGGCATGC CAGTTATGCC AGTAGGCACA CCTGGGCAGG TTTCTACCCC AGGCACACAC
    GCTTCAGCCC CAGTCGGCAC TGCAACAGGA GTAAAACCTG GAACAACTCC CCCAAAGCCA
    ACTAAGACTG TGGTGCCACC AGTGGGTACT GAACTTTCAG CCGGTACTGT ACCCTGTGAG
    CAGCTGCCAC CTTTTCCTGG ACCTTCTCTA ATTCAGTCCC AGCAACCACT AGAAGATCTT
    GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTA CAGTGGCACC TGCAGTTGGT
    CCTTTGTCTA CGATGTCTTC AACAACAGTT ACAGAAGCAG GGACTCGGCT TCAAAAGGAT
    GGCACAGAAG GTCATGTCAC AGCAACTAGT TCAGGAGCTG GTGTTGTTAA GATGGGGCGA
    TTTCAGGTTT CAGTTACAAT GGATGATGCC CAGAAAGAAC GTAAAAATAG GTCAGAAGAT
    ACAAAGTCGG TTCATTTTGA ATCTAGCACA TCAGAGTCAT CAGTTCTTTC AAGTAGTAGT
    CCAGAGAGTA CCCTGGTAAA GCCAGAGCCT AATGGAATTA GTATTTCTGG CATCTCCTTA
    GATGTCCCAG ACAGCACCCA CAAAGCACCT ACCCCAGAAG CAAAGTCAGA TGCAGGACAG
    CCTACCAAGG TGGGACGTTT TCAGGTGACA ACTACAGCCA ACAAAGTGGG TCGTTTCTCT
    GTTTCAAGAA CTGAGGACAA GGTCACAGAG CTCAAAAAAG AGGGACCAGT GACCTCTCCT
    CCTTTCAGAG ATTCAGAACA AACTGTTATT CCTGCTGTGA TACCAAAGAA GGAGAAGCCT
    GAGCTGGCAG AACCTTCCCA TTTGAATGGG CCATCTTCAG ACCTGGAGGC TGCCTTTCTA
    AGTAGGGGTA CAGAGGATGG CTCAGGTAGT CCACACTCTC CCCCTCACCT GTGTTCAAAG
    AGCCTTCCCG TCCAGAATCT AAGTCAAAGC CTTAGTAATT CATTCAACTC CTCATACATG
    AGTAGTGACA ATGAGTCCGA CATTGAAGAT GAAGACTTAA GGTTAGAACT ACGACGACTA
    CGAGAAAAAC ATCTTAAAGA GATTCAGGAC CTGCAGAGTC GCCAGAAGCA TGAAATTGAA
    TCTTTGTATA CTAAACTGGG CAAGGTTCCC CCTGCTGTCA TTATTCCCCC AGCTGCTCCT
    CTGTCGGGGA GAAGAAGGAG ACCCACTAAA AGCAAAGGCA GCAAGTCTAG TCGCAGCAGC
    TCATTGGGCA ATAAAAGCCC ACAGCTTTCA GGCAACCTGT CTGGTCAGAG TGGAACTTCA
    GTCTTACACC CCCAACAGAC CCTCCATCCC GCAGGAAACA CCCCCGAGAC TGGGCACAAC
    CAGCTGTTAC AGCCTCTTAA GCCATCTCCC TCCAGTGACA ACCTCTATTC AGCCTTTACC
    AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GTCAAGGGAC CAGCAGCACA
    AATACCGTTG GAGGAACAGT GAGTAGCCAG GCTGCCCAGG CTCAGCCTCC TGCTATGACT
    TCCAGTAGGA AGGGCACATT CACAGATGAC CTGCACAAGT TAGTAGACAA CTGGGCCCGA
    GATGCCATGA ATCTTTCAGG CAGGAGAGGC AGCAAAGGAC ACATGAATTA TGAGGGCCCT
    GGAATGGCAA GGAAGTTCTC TGCTCCTGGG CAGCTGTGTG TTCCCATGAC CTCAAACCTG
    GGTGGCTCTA CCCCCATCTC TGCAGCATCT GCTACCTCTC TAGGTCACTT CACCAAATCC
    ATGTGTCCCC CACAGCAGTA TGGTTTTCCA CCTGCCCCAT TTGGCACTCA GTGGAGTGGA
    ACAGGTGGTC CAGCACCGCA GCCACTTGGC CAGTTCCAAC CTGTAGGAAC AGCATCCTTG
    CAAAATTTCA ACATCAGCAA TTTGCAGAAG TCCATCAGCA ACCCCCCAGG TTCCAATCTA
    CGGACCACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81713
    Clone ID Related Accession (Same CDS sequence) XM_017321555.1
    Accession Version XM_017321555.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGA GTACTCAGGG AGTCTCTCAG GCTGCTCCTC CAGAACAAAC TCCAATAACA
    CAGTCACAAC CTACTCAGCC TGTTCCATTG GTTACCTCTG CAGACAGTGC ACATTCAGAC
    GTTGCTTCTG GTATGAGTGA TGGCAATGAG AATGCTCCAT CATCCAGCGG GAGGCATGAA
    GGGAGGACAA CAAAACGGCA TTATAGAAAA TCCGTAAGAA GTCGCTCTCG GCATGAAAAG
    ACTTCTCGCC CAAAACTGAG GATTTTAAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TCGGAAGATG GTTACATTTA AATTTGACCT AGATGGTGAC
    AACCCTGAAG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCTTTTG TGGCTCAAGT GCGGGAAATT ATTGAAAAGG CTGATGAAAT GTTAAGTGAG
    GATGTCAGTG TGGAGCCTGA GGGTGACCAG GGATTGGAGA GTCTGCAAGG AAAGGATGAC
    TATGGCTTCC CAGGTTCTCA GAAATTAGAA GGAGAGTTCA AGCAACCAAT TGCTGTATCT
    TCCATGCCAC AACAAATAGG TGTTCCTACC AGTTCTTTAA CGCAAGTTGT TCATTCTGCT
    GGAAGACGGT TTATAGTGAG TCCTGTACCT GAAAGTAGGT TACGAGAATC AAAAGTTTTC
    ACCAGTGACA TATCAGATCC AGTTGTGGCC TCTACATCTC AGGCCCCTGG AATGAACTTA
    TCTCACTCTG CTTCATCCCT CAGTCTCCAG CAGGCTTTTT CTGAACTGAA ACATGGTCAG
    ATGACTGAAG GACCCAATAC AGCACCTCCA AATTTTAATC ACATGGCGGG ACCAACATTT
    TCTCCCTTTT TGGCTAGCAT TGCTGGTGTC CAAACTGTAG CAGCATCCAC ACCGTCAGTA
    TCAGTCCCTA TAACAAGCAG TCCTCTTAAT GACATTTCCA CATCAGTGAT GCAGTCTGAG
    ACTGCACTGC CCACTGAAAA AGGCATCGTG GGAGTTACCA CTACCAGCAC AGGTGTGGTA
    GCTTCAGGTG GTCTCACCAC AATGTCTGTG TCTGAGTCAC CAACATCTTC CAGTGCGGTT
    TCAAGTTCTA CAGTACCTGC AGTTGTCACT GTGTCCACAC CATCCCAGCC AGTTCAGGCT
    TCCACATCTG GGAGCATTGC TTCAAGTACA GGCAGTTTTC CTCCTGGAAC ATTTTCTACA
    ACTACAGCTA CTACAATGGG TAGTGTGGTG GCCCCAGATG CTAAGCCTCC AACTGTATTA
    TTGCAACAGG TAGCAAGCAA TACTGCTGGG GTTGCCATAG TGACATCAGT CTCTACCACC
    ACTCCATTCC CAGGCATGGC TTCACAGCCA TCCCTTCCAC TTAGTAGCAG CACCTCTGCT
    CCTACTCTAG CTGAAACAAT GGTGGTTAGT GCACACTCAC TAGACAAGGC ATCTCACAGT
    AGTACAGCAG GATTGGGTTT GTCCTTTTGT GCGCCATCCT CTTCCTCCTC CTCTGGAACA
    GCTGTGTCTA CTTCTGTTTC TCAGCCTGGT ATGGTTCATC CTTTGGTCAT CTCATCAGCT
    GTAGTTTCTA CACCTGGCCT CCCCCAACCA GTAGTACCTA CTTCTACACC TTTATTACCC
    CAAGTACCTA ATATCCCACC CTTGGTACAA CCTGTTGTTA ATGTGCCTGC TGTACAGCAA
    ACACTCATTC ACAGTCAGCC TCAACCAGCT CTGCTTCCCA ACCAGCCTCA CACACACTGT
    CCTGAAATGG ATGCTGATAC CCAATCCAAA GCTCCTGGGA TTGATGATAT AAAGACTCTG
    GAGGAAAAGC TGAGATCTCT CTTTAGTGAA CACAGCTCAT CTGGAACTCA GCATGCTTCT
    GTCTCCCTAG AGACACCTTT AGTAGTAGAG ACGACTGTCA CACCGGGCAT CACTACCACT
    GCTGTAGCAC CAAGTAAACT CATGACTTCC ACTACAAGTA CTTGCTTACC ACCAACCAGT
    TTACCATTAG GAGCAGCTGG CATGCCAGTT ATGCCAGTAG GCACACCTGG GCAGGTTTCT
    ACCCCAGGCA CACACGCTTC AGCCCCAGTC GGCACTGCAA CAGGAGTAAA ACCTGGAACA
    ACTCCCCCAA AGCCAACTAA GACTGTGGTG CCACCAGTGG GTACTGAACT TTCAGCCGGT
    ACTGTACCCT GTGAGCAGCT GCCACCTTTT CCTGGACCTT CTCTAATTCA GTCCCAGCAA
    CCACTAGAAG ATCTTGATGC TCAGTTGAGA AGAACACTTA GTCCAGAGAC TATTACAGTG
    GCACCTGCAG TTGGTCCTTT GTCTACGATG TCTTCAACAA CAGTTACAGA AGCAGGGACT
    CGGCTTCAAA AGGATGGCAC AGAAGGTCAT GTCACAGCAA CTAGTTCAGG AGCTGGTGTT
    GTTAAGATGG GGCGATTTCA GGTTTCAGTT ACAATGGATG ATGCCCAGAA AGAACGTAAA
    AATAGGTCAG AAGATACAAA GTCGGTTCAT TTTGAATCTA GCACATCAGA GTCATCAGTT
    CTTTCAAGTA GTAGTCCAGA GAGTACCCTG GTAAAGCCAG AGCCTAATGG AATTAGTATT
    TCTGGCATCT CCTTAGATGT CCCAGACAGC ACCCACAAAG CACCTACCCC AGAAGCAAAG
    TCAGATGCAG GACAGCCTAC CAAGGTGGGA CGTTTTCAGG TGACAACTAC AGCCAACAAA
    GTGGGTCGTT TCTCTGTTTC AAGAACTGAG GACAAGGTCA CAGAGCTCAA AAAAGAGGGA
    CCAGTGACCT CTCCTCCTTT CAGAGATTCA GAACAAACTG TTATTCCTGC TGTGATACCA
    AAGAAGGAGA AGCCTGAGCT GGCAGAACCT TCCCATTTGA ATGGGCCATC TTCAGACCTG
    GAGGCTGCCT TTCTAAGTAG GGGTACAGAG GATGGCTCAG GTAGTCCACA CTCTCCCCCT
    CACCTGTGTT CAAAGAGCCT TCCCGTCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTC
    AACTCCTCAT ACATGAGTAG TGACAATGAG TCCGACATTG AAGATGAAGA CTTAAGGTTA
    GAACTACGAC GACTACGAGA AAAACATCTT AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACTAAA CTGGGCAAGG TTCCCCCTGC TGTCATTATT
    CCCCCAGCTG CTCCTCTGTC GGGGAGAAGA AGGAGACCCA CTAAAAGCAA AGGCAGCAAG
    TCTAGTCGCA GCAGCTCATT GGGCAATAAA AGCCCACAGC TTTCAGGCAA CCTGTCTGGT
    CAGAGTGGAA CTTCAGTCTT ACACCCCCAA CAGACCCTCC ATCCCGCAGG AAACACCCCC
    GAGACTGGGC ACAACCAGCT GTTACAGCCT CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TTACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGCTGTGCGA AGTTTAACTG TGCATCTGAG CAGGTGACAT TCAAACCTGG TGGCAGGAGG
    ACCCGATTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT
    TGCAACCAGC TCTGTATGTG CCCTCCAGCT GAACCGAAAT CAGGGACCAG CAGCACAAAT
    ACCGTTGGAG GAACAGTGAG TAGCCAGGCT GCCCAGGCTC AGCCTCCTGC TATGACTTCC
    AGTAGGAAGG GCACATTCAC AGATGACCTG CACAAGTTAG TAGACAACTG GGCCCGAGAT
    GCCATGAATC TTTCAGGCAG GAGAGGCAGC AAAGGACACA TGAATTATGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC TCCTGGGCAG CTGTGTGTTC CCATGACCTC AAACCTGGGT
    GGCTCTACCC CCATCTCTGC AGCATCTGCT ACCTCTCTAG GTCACTTCAC CAAATCCATG
    TGTCCCCCAC AGCAGTATGG TTTTCCACCT GCCCCATTTG GCACTCAGTG GAGTGGAACA
    GGTGGTCCAG CACCGCAGCC ACTTGGCCAG TTCCAACCTG TAGGAACAGC ATCCTTGCAA
    AATTTCAACA TCAGCAATTT GCAGAAGTCC ATCAGCAACC CCCCAGGTTC CAATCTACGG
    ACCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177044.1
    CDS885..7433
    Translation

    Target ORF information:

    RefSeq Version XM_017321555.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321555.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGA GTACTCAGGG AGTCTCTCAG GCTGCTCCTC CAGAACAAAC TCCAATAACA
    CAGTCACAAC CTACTCAGCC TGTTCCATTG GTTACCTCTG CAGACAGTGC ACATTCAGAC
    GTTGCTTCTG GTATGAGTGA TGGCAATGAG AATGCTCCAT CATCCAGCGG GAGGCATGAA
    GGGAGGACAA CAAAACGGCA TTATAGAAAA TCCGTAAGAA GTCGCTCTCG GCATGAAAAG
    ACTTCTCGCC CAAAACTGAG GATTTTAAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TCGGAAGATG GTTACATTTA AATTTGACCT AGATGGTGAC
    AACCCTGAAG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCTTTTG TGGCTCAAGT GCGGGAAATT ATTGAAAAGG CTGATGAAAT GTTAAGTGAG
    GATGTCAGTG TGGAGCCTGA GGGTGACCAG GGATTGGAGA GTCTGCAAGG AAAGGATGAC
    TATGGCTTCC CAGGTTCTCA GAAATTAGAA GGAGAGTTCA AGCAACCAAT TGCTGTATCT
    TCCATGCCAC AACAAATAGG TGTTCCTACC AGTTCTTTAA CGCAAGTTGT TCATTCTGCT
    GGAAGACGGT TTATAGTGAG TCCTGTACCT GAAAGTAGGT TACGAGAATC AAAAGTTTTC
    ACCAGTGACA TATCAGATCC AGTTGTGGCC TCTACATCTC AGGCCCCTGG AATGAACTTA
    TCTCACTCTG CTTCATCCCT CAGTCTCCAG CAGGCTTTTT CTGAACTGAA ACATGGTCAG
    ATGACTGAAG GACCCAATAC AGCACCTCCA AATTTTAATC ACATGGCGGG ACCAACATTT
    TCTCCCTTTT TGGCTAGCAT TGCTGGTGTC CAAACTGTAG CAGCATCCAC ACCGTCAGTA
    TCAGTCCCTA TAACAAGCAG TCCTCTTAAT GACATTTCCA CATCAGTGAT GCAGTCTGAG
    ACTGCACTGC CCACTGAAAA AGGCATCGTG GGAGTTACCA CTACCAGCAC AGGTGTGGTA
    GCTTCAGGTG GTCTCACCAC AATGTCTGTG TCTGAGTCAC CAACATCTTC CAGTGCGGTT
    TCAAGTTCTA CAGTACCTGC AGTTGTCACT GTGTCCACAC CATCCCAGCC AGTTCAGGCT
    TCCACATCTG GGAGCATTGC TTCAAGTACA GGCAGTTTTC CTCCTGGAAC ATTTTCTACA
    ACTACAGCTA CTACAATGGG TAGTGTGGTG GCCCCAGATG CTAAGCCTCC AACTGTATTA
    TTGCAACAGG TAGCAAGCAA TACTGCTGGG GTTGCCATAG TGACATCAGT CTCTACCACC
    ACTCCATTCC CAGGCATGGC TTCACAGCCA TCCCTTCCAC TTAGTAGCAG CACCTCTGCT
    CCTACTCTAG CTGAAACAAT GGTGGTTAGT GCACACTCAC TAGACAAGGC ATCTCACAGT
    AGTACAGCAG GATTGGGTTT GTCCTTTTGT GCGCCATCCT CTTCCTCCTC CTCTGGAACA
    GCTGTGTCTA CTTCTGTTTC TCAGCCTGGT ATGGTTCATC CTTTGGTCAT CTCATCAGCT
    GTAGTTTCTA CACCTGGCCT CCCCCAACCA GTAGTACCTA CTTCTACACC TTTATTACCC
    CAAGTACCTA ATATCCCACC CTTGGTACAA CCTGTTGTTA ATGTGCCTGC TGTACAGCAA
    ACACTCATTC ACAGTCAGCC TCAACCAGCT CTGCTTCCCA ACCAGCCTCA CACACACTGT
    CCTGAAATGG ATGCTGATAC CCAATCCAAA GCTCCTGGGA TTGATGATAT AAAGACTCTG
    GAGGAAAAGC TGAGATCTCT CTTTAGTGAA CACAGCTCAT CTGGAACTCA GCATGCTTCT
    GTCTCCCTAG AGACACCTTT AGTAGTAGAG ACGACTGTCA CACCGGGCAT CACTACCACT
    GCTGTAGCAC CAAGTAAACT CATGACTTCC ACTACAAGTA CTTGCTTACC ACCAACCAGT
    TTACCATTAG GAGCAGCTGG CATGCCAGTT ATGCCAGTAG GCACACCTGG GCAGGTTTCT
    ACCCCAGGCA CACACGCTTC AGCCCCAGTC GGCACTGCAA CAGGAGTAAA ACCTGGAACA
    ACTCCCCCAA AGCCAACTAA GACTGTGGTG CCACCAGTGG GTACTGAACT TTCAGCCGGT
    ACTGTACCCT GTGAGCAGCT GCCACCTTTT CCTGGACCTT CTCTAATTCA GTCCCAGCAA
    CCACTAGAAG ATCTTGATGC TCAGTTGAGA AGAACACTTA GTCCAGAGAC TATTACAGTG
    GCACCTGCAG TTGGTCCTTT GTCTACGATG TCTTCAACAA CAGTTACAGA AGCAGGGACT
    CGGCTTCAAA AGGATGGCAC AGAAGGTCAT GTCACAGCAA CTAGTTCAGG AGCTGGTGTT
    GTTAAGATGG GGCGATTTCA GGTTTCAGTT ACAATGGATG ATGCCCAGAA AGAACGTAAA
    AATAGGTCAG AAGATACAAA GTCGGTTCAT TTTGAATCTA GCACATCAGA GTCATCAGTT
    CTTTCAAGTA GTAGTCCAGA GAGTACCCTG GTAAAGCCAG AGCCTAATGG AATTAGTATT
    TCTGGCATCT CCTTAGATGT CCCAGACAGC ACCCACAAAG CACCTACCCC AGAAGCAAAG
    TCAGATGCAG GACAGCCTAC CAAGGTGGGA CGTTTTCAGG TGACAACTAC AGCCAACAAA
    GTGGGTCGTT TCTCTGTTTC AAGAACTGAG GACAAGGTCA CAGAGCTCAA AAAAGAGGGA
    CCAGTGACCT CTCCTCCTTT CAGAGATTCA GAACAAACTG TTATTCCTGC TGTGATACCA
    AAGAAGGAGA AGCCTGAGCT GGCAGAACCT TCCCATTTGA ATGGGCCATC TTCAGACCTG
    GAGGCTGCCT TTCTAAGTAG GGGTACAGAG GATGGCTCAG GTAGTCCACA CTCTCCCCCT
    CACCTGTGTT CAAAGAGCCT TCCCGTCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTC
    AACTCCTCAT ACATGAGTAG TGACAATGAG TCCGACATTG AAGATGAAGA CTTAAGGTTA
    GAACTACGAC GACTACGAGA AAAACATCTT AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACTAAA CTGGGCAAGG TTCCCCCTGC TGTCATTATT
    CCCCCAGCTG CTCCTCTGTC GGGGAGAAGA AGGAGACCCA CTAAAAGCAA AGGCAGCAAG
    TCTAGTCGCA GCAGCTCATT GGGCAATAAA AGCCCACAGC TTTCAGGCAA CCTGTCTGGT
    CAGAGTGGAA CTTCAGTCTT ACACCCCCAA CAGACCCTCC ATCCCGCAGG AAACACCCCC
    GAGACTGGGC ACAACCAGCT GTTACAGCCT CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TTACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGCTGTGCGA AGTTTAACTG TGCATCTGAG CAGGTGACAT TCAAACCTGG TGGCAGGAGG
    ACCCGATTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT
    TGCAACCAGC TCTGTATGTG CCCTCCAGCT GAACCGAAAT CAGGGACCAG CAGCACAAAT
    ACCGTTGGAG GAACAGTGAG TAGCCAGGCT GCCCAGGCTC AGCCTCCTGC TATGACTTCC
    AGTAGGAAGG GCACATTCAC AGATGACCTG CACAAGTTAG TAGACAACTG GGCCCGAGAT
    GCCATGAATC TTTCAGGCAG GAGAGGCAGC AAAGGACACA TGAATTATGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC TCCTGGGCAG CTGTGTGTTC CCATGACCTC AAACCTGGGT
    GGCTCTACCC CCATCTCTGC AGCATCTGCT ACCTCTCTAG GTCACTTCAC CAAATCCATG
    TGTCCCCCAC AGCAGTATGG TTTTCCACCT GCCCCATTTG GCACTCAGTG GAGTGGAACA
    GGTGGTCCAG CACCGCAGCC ACTTGGCCAG TTCCAACCTG TAGGAACAGC ATCCTTGCAA
    AATTTCAACA TCAGCAATTT GCAGAAGTCC ATCAGCAACC CCCCAGGTTC CAATCTACGG
    ACCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81714
    Clone ID Related Accession (Same CDS sequence) XM_017321556.1
    Accession Version XM_017321556.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGGGAATAC AGCCAACTGT TCCTTCTCAA CAGGCAGTAC AGTATTCACT TCCACAAGCA
    GCATCTTCCA GTGAAGGTAC TACTGCACAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGAGTA CTCAGGGAGT CTCTCAGGCT GCTCCTCCAG AACAAACTCC AATAACACAG
    TCACAACCTA CTCAGCCTGT TCCATTGGTT ACCTCTGCAG ACAGTGCACA TTCAGACGTT
    GCTTCTGGTA TGAGTGATGG CAATGAGAAT GCTCCATCAT CCAGCGGGAG GCATGAAGGG
    AGGACAACAA AACGGCATTA TAGAAAATCC GTAAGAAGTC GCTCTCGGCA TGAAAAGACT
    TCTCGCCCAA AACTGAGGAT TTTAAATGTT TCAAATAAAG GAGACCGAGT AGTAGAATGT
    CAATTAGAGA CTCATAATCG GAAGATGGTT ACATTTAAAT TTGACCTAGA TGGTGACAAC
    CCTGAAGAGA TAGCAACAAT TATGGTGAAC AATGACTTTA TTCTAGCAAT AGAGAGAGAG
    TCTTTTGTGG CTCAAGTGCG GGAAATTATT GAAAAGGCTG ATGAAATGTT AAGTGAGGAT
    GTCAGTGTGG AGCCTGAGGG TGACCAGGGA TTGGAGAGTC TGCAAGGAAA GGATGACTAT
    GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA GAGTTCAAGC AACCAATTGC TGTATCTTCC
    ATGCCACAAC AAATAGGTGT TCCTACCAGT TCTTTAACGC AAGTTGTTCA TTCTGCTGGA
    AGACGGTTTA TAGTGAGTCC TGTACCTGAA AGTAGGTTAC GAGAATCAAA AGTTTTCACC
    AGTGACATAT CAGATCCAGT TGTGGCCTCT ACATCTCAGG CCCCTGGAAT GAACTTATCT
    CACTCTGCTT CATCCCTCAG TCTCCAGCAG GCTTTTTCTG AACTGAAACA TGGTCAGATG
    ACTGAAGGAC CCAATACAGC ACCTCCAAAT TTTAATCACA TGGCGGGACC AACATTTTCT
    CCCTTTTTGG CTAGCATTGC TGGTGTCCAA ACTGTAGCAG CATCCACACC GTCAGTATCA
    GTCCCTATAA CAAGCAGTCC TCTTAATGAC ATTTCCACAT CAGTGATGCA GTCTGAGACT
    GCACTGCCCA CTGAAAAAGG CATCGTGGGA GTTACCACTA CCAGCACAGG TGTGGTAGCT
    TCAGGTGGTC TCACCACAAT GTCTGTGTCT GAGTCACCAA CATCTTCCAG TGCGGTTTCA
    AGTTCTACAG TACCTGCAGT TGTCACTGTG TCCACACCAT CCCAGCCAGT TCAGGCTTCC
    ACATCTGGGA GCATTGCTTC AAGTACAGGC AGTTTTCCTC CTGGAACATT TTCTACAACT
    ACAGCTACTA CAATGGGTAG TGTGGTGGCC CCAGATGCTA AGCCTCCAAC TGTATTATTG
    CAACAGGTAG CAAGCAATAC TGCTGGGGTT GCCATAGTGA CATCAGTCTC TACCACCACT
    CCATTCCCAG GCATGGCTTC ACAGCCATCC CTTCCACTTA GTAGCAGCAC CTCTGCTCCT
    ACTCTAGCTG AAACAATGGT GGTTAGTGCA CACTCACTAG ACAAGGCATC TCACAGTAGT
    ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG CCATCCTCTT CCTCCTCCTC TGGAACAGCT
    GTGTCTACTT CTGTTTCTCA GCCTGGTATG GTTCATCCTT TGGTCATCTC ATCAGCTGTA
    GTTTCTACAC CTGGCCTCCC CCAACCAGTA GTACCTACTT CTACACCTTT ATTACCCCAA
    GTACCTAATA TCCCACCCTT GGTACAACCT GTTGTTAATG TGCCTGCTGT ACAGCAAACA
    CTCATTCACA GTCAGCCTCA ACCAGCTCTG CTTCCCAACC AGCCTCACAC ACACTGTCCT
    GAAATGGATG CTGATACCCA ATCCAAAGCT CCTGGGATTG ATGATATAAA GACTCTGGAG
    GAAAAGCTGA GATCTCTCTT TAGTGAACAC AGCTCATCTG GAACTCAGCA TGCTTCTGTC
    TCCCTAGAGA CACCTTTAGT AGTAGAGACG ACTGTCACAC CGGGCATCAC TACCACTGCT
    GTAGCACCAA GTAAACTCAT GACTTCCACT ACAAGTACTT GCTTACCACC AACCAGTTTA
    CCATTAGGAG CAGCTGGCAT GCCAGTTATG CCAGTAGGCA CACCTGGGCA GGTTTCTACC
    CCAGGCACAC ACGCTTCAGC CCCAGTCGGC ACTGCAACAG GAGTAAAACC TGGAACAACT
    CCCCCAAAGC CAACTAAGAC TGTGGTGCCA CCAGTGGGTA CTGAACTTTC AGCCGGTACT
    GTACCCTGTG AGCAGCTGCC ACCTTTTCCT GGACCTTCTC TAATTCAGTC CCAGCAACCA
    CTAGAAGATC TTGATGCTCA GTTGAGAAGA ACACTTAGTC CAGAGACTAT TACAGTGGCA
    CCTGCAGTTG GTCCTTTGTC TACGATGTCT TCAACAACAG TTACAGAAGC AGGGACTCGG
    CTTCAAAAGG ATGGCACAGA AGGTCATGTC ACAGCAACTA GTTCAGGAGC TGGTGTTGTT
    AAGATGGGGC GATTTCAGGT TTCAGTTACA ATGGATGATG CCCAGAAAGA ACGTAAAAAT
    AGGTCAGAAG ATACAAAGTC GGTTCATTTT GAATCTAGCA CATCAGAGTC ATCAGTTCTT
    TCAAGTAGTA GTCCAGAGAG TACCCTGGTA AAGCCAGAGC CTAATGGAAT TAGTATTTCT
    GGCATCTCCT TAGATGTCCC AGACAGCACC CACAAAGCAC CTACCCCAGA AGCAAAGTCA
    GATGCAGGAC AGCCTACCAA GGTGGGACGT TTTCAGGTGA CAACTACAGC CAACAAAGTG
    GGTCGTTTCT CTGTTTCAAG AACTGAGGAC AAGGTCACAG AGCTCAAAAA AGAGGGACCA
    GTGACCTCTC CTCCTTTCAG AGATTCAGAA CAAACTGTTA TTCCTGCTGT GATACCAAAG
    AAGGAGAAGC CTGAGCTGGC AGAACCTTCC CATTTGAATG GGCCATCTTC AGACCTGGAG
    GCTGCCTTTC TAAGTAGGGG TACAGAGGAT GGCTCAGGTA GTCCACACTC TCCCCCTCAC
    CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCATACA TGAGTAGTGA CAATGAGTCC GACATTGAAG ATGAAGACTT AAGGTTAGAA
    CTACGACGAC TACGAGAAAA ACATCTTAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACTAAACTG GGCAAGGTTC CCCCTGCTGT CATTATTCCC
    CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG AGACCCACTA AAAGCAAAGG CAGCAAGTCT
    AGTCGCAGCA GCTCATTGGG CAATAAAAGC CCACAGCTTT CAGGCAACCT GTCTGGTCAG
    AGTGGAACTT CAGTCTTACA CCCCCAACAG ACCCTCCATC CCGCAGGAAA CACCCCCGAG
    ACTGGGCACA ACCAGCTGTT ACAGCCTCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAT
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTACCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC
    AACCAGCTCT GTATGTGCCC TCCAGCTGAA CCGAAATCAG GGACCAGCAG CACAAATACC
    GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC CAGGCTCAGC CTCCTGCTAT GACTTCCAGT
    AGGAAGGGCA CATTCACAGA TGACCTGCAC AAGTTAGTAG ACAACTGGGC CCGAGATGCC
    ATGAATCTTT CAGGCAGGAG AGGCAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG TGTGTTCCCA TGACCTCAAA CCTGGGTGGC
    TCTACCCCCA TCTCTGCAGC ATCTGCTACC TCTCTAGGTC ACTTCACCAA ATCCATGTGT
    CCCCCACAGC AGTATGGTTT TCCACCTGCC CCATTTGGCA CTCAGTGGAG TGGAACAGGT
    GGTCCAGCAC CGCAGCCACT TGGCCAGTTC CAACCTGTAG GAACAGCATC CTTGCAAAAT
    TTCAACATCA GCAATTTGCA GAAGTCCATC AGCAACCCCC CAGGTTCCAA TCTACGGACC
    ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177045.1
    CDS885..7430
    Translation

    Target ORF information:

    RefSeq Version XM_017321556.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGGGAATAC AGCCAACTGT TCCTTCTCAA CAGGCAGTAC AGTATTCACT TCCACAAGCA
    GCATCTTCCA GTGAAGGTAC TACTGCACAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGAGTA CTCAGGGAGT CTCTCAGGCT GCTCCTCCAG AACAAACTCC AATAACACAG
    TCACAACCTA CTCAGCCTGT TCCATTGGTT ACCTCTGCAG ACAGTGCACA TTCAGACGTT
    GCTTCTGGTA TGAGTGATGG CAATGAGAAT GCTCCATCAT CCAGCGGGAG GCATGAAGGG
    AGGACAACAA AACGGCATTA TAGAAAATCC GTAAGAAGTC GCTCTCGGCA TGAAAAGACT
    TCTCGCCCAA AACTGAGGAT TTTAAATGTT TCAAATAAAG GAGACCGAGT AGTAGAATGT
    CAATTAGAGA CTCATAATCG GAAGATGGTT ACATTTAAAT TTGACCTAGA TGGTGACAAC
    CCTGAAGAGA TAGCAACAAT TATGGTGAAC AATGACTTTA TTCTAGCAAT AGAGAGAGAG
    TCTTTTGTGG CTCAAGTGCG GGAAATTATT GAAAAGGCTG ATGAAATGTT AAGTGAGGAT
    GTCAGTGTGG AGCCTGAGGG TGACCAGGGA TTGGAGAGTC TGCAAGGAAA GGATGACTAT
    GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA GAGTTCAAGC AACCAATTGC TGTATCTTCC
    ATGCCACAAC AAATAGGTGT TCCTACCAGT TCTTTAACGC AAGTTGTTCA TTCTGCTGGA
    AGACGGTTTA TAGTGAGTCC TGTACCTGAA AGTAGGTTAC GAGAATCAAA AGTTTTCACC
    AGTGACATAT CAGATCCAGT TGTGGCCTCT ACATCTCAGG CCCCTGGAAT GAACTTATCT
    CACTCTGCTT CATCCCTCAG TCTCCAGCAG GCTTTTTCTG AACTGAAACA TGGTCAGATG
    ACTGAAGGAC CCAATACAGC ACCTCCAAAT TTTAATCACA TGGCGGGACC AACATTTTCT
    CCCTTTTTGG CTAGCATTGC TGGTGTCCAA ACTGTAGCAG CATCCACACC GTCAGTATCA
    GTCCCTATAA CAAGCAGTCC TCTTAATGAC ATTTCCACAT CAGTGATGCA GTCTGAGACT
    GCACTGCCCA CTGAAAAAGG CATCGTGGGA GTTACCACTA CCAGCACAGG TGTGGTAGCT
    TCAGGTGGTC TCACCACAAT GTCTGTGTCT GAGTCACCAA CATCTTCCAG TGCGGTTTCA
    AGTTCTACAG TACCTGCAGT TGTCACTGTG TCCACACCAT CCCAGCCAGT TCAGGCTTCC
    ACATCTGGGA GCATTGCTTC AAGTACAGGC AGTTTTCCTC CTGGAACATT TTCTACAACT
    ACAGCTACTA CAATGGGTAG TGTGGTGGCC CCAGATGCTA AGCCTCCAAC TGTATTATTG
    CAACAGGTAG CAAGCAATAC TGCTGGGGTT GCCATAGTGA CATCAGTCTC TACCACCACT
    CCATTCCCAG GCATGGCTTC ACAGCCATCC CTTCCACTTA GTAGCAGCAC CTCTGCTCCT
    ACTCTAGCTG AAACAATGGT GGTTAGTGCA CACTCACTAG ACAAGGCATC TCACAGTAGT
    ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG CCATCCTCTT CCTCCTCCTC TGGAACAGCT
    GTGTCTACTT CTGTTTCTCA GCCTGGTATG GTTCATCCTT TGGTCATCTC ATCAGCTGTA
    GTTTCTACAC CTGGCCTCCC CCAACCAGTA GTACCTACTT CTACACCTTT ATTACCCCAA
    GTACCTAATA TCCCACCCTT GGTACAACCT GTTGTTAATG TGCCTGCTGT ACAGCAAACA
    CTCATTCACA GTCAGCCTCA ACCAGCTCTG CTTCCCAACC AGCCTCACAC ACACTGTCCT
    GAAATGGATG CTGATACCCA ATCCAAAGCT CCTGGGATTG ATGATATAAA GACTCTGGAG
    GAAAAGCTGA GATCTCTCTT TAGTGAACAC AGCTCATCTG GAACTCAGCA TGCTTCTGTC
    TCCCTAGAGA CACCTTTAGT AGTAGAGACG ACTGTCACAC CGGGCATCAC TACCACTGCT
    GTAGCACCAA GTAAACTCAT GACTTCCACT ACAAGTACTT GCTTACCACC AACCAGTTTA
    CCATTAGGAG CAGCTGGCAT GCCAGTTATG CCAGTAGGCA CACCTGGGCA GGTTTCTACC
    CCAGGCACAC ACGCTTCAGC CCCAGTCGGC ACTGCAACAG GAGTAAAACC TGGAACAACT
    CCCCCAAAGC CAACTAAGAC TGTGGTGCCA CCAGTGGGTA CTGAACTTTC AGCCGGTACT
    GTACCCTGTG AGCAGCTGCC ACCTTTTCCT GGACCTTCTC TAATTCAGTC CCAGCAACCA
    CTAGAAGATC TTGATGCTCA GTTGAGAAGA ACACTTAGTC CAGAGACTAT TACAGTGGCA
    CCTGCAGTTG GTCCTTTGTC TACGATGTCT TCAACAACAG TTACAGAAGC AGGGACTCGG
    CTTCAAAAGG ATGGCACAGA AGGTCATGTC ACAGCAACTA GTTCAGGAGC TGGTGTTGTT
    AAGATGGGGC GATTTCAGGT TTCAGTTACA ATGGATGATG CCCAGAAAGA ACGTAAAAAT
    AGGTCAGAAG ATACAAAGTC GGTTCATTTT GAATCTAGCA CATCAGAGTC ATCAGTTCTT
    TCAAGTAGTA GTCCAGAGAG TACCCTGGTA AAGCCAGAGC CTAATGGAAT TAGTATTTCT
    GGCATCTCCT TAGATGTCCC AGACAGCACC CACAAAGCAC CTACCCCAGA AGCAAAGTCA
    GATGCAGGAC AGCCTACCAA GGTGGGACGT TTTCAGGTGA CAACTACAGC CAACAAAGTG
    GGTCGTTTCT CTGTTTCAAG AACTGAGGAC AAGGTCACAG AGCTCAAAAA AGAGGGACCA
    GTGACCTCTC CTCCTTTCAG AGATTCAGAA CAAACTGTTA TTCCTGCTGT GATACCAAAG
    AAGGAGAAGC CTGAGCTGGC AGAACCTTCC CATTTGAATG GGCCATCTTC AGACCTGGAG
    GCTGCCTTTC TAAGTAGGGG TACAGAGGAT GGCTCAGGTA GTCCACACTC TCCCCCTCAC
    CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCATACA TGAGTAGTGA CAATGAGTCC GACATTGAAG ATGAAGACTT AAGGTTAGAA
    CTACGACGAC TACGAGAAAA ACATCTTAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACTAAACTG GGCAAGGTTC CCCCTGCTGT CATTATTCCC
    CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG AGACCCACTA AAAGCAAAGG CAGCAAGTCT
    AGTCGCAGCA GCTCATTGGG CAATAAAAGC CCACAGCTTT CAGGCAACCT GTCTGGTCAG
    AGTGGAACTT CAGTCTTACA CCCCCAACAG ACCCTCCATC CCGCAGGAAA CACCCCCGAG
    ACTGGGCACA ACCAGCTGTT ACAGCCTCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAT
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTACCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC
    AACCAGCTCT GTATGTGCCC TCCAGCTGAA CCGAAATCAG GGACCAGCAG CACAAATACC
    GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC CAGGCTCAGC CTCCTGCTAT GACTTCCAGT
    AGGAAGGGCA CATTCACAGA TGACCTGCAC AAGTTAGTAG ACAACTGGGC CCGAGATGCC
    ATGAATCTTT CAGGCAGGAG AGGCAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG TGTGTTCCCA TGACCTCAAA CCTGGGTGGC
    TCTACCCCCA TCTCTGCAGC ATCTGCTACC TCTCTAGGTC ACTTCACCAA ATCCATGTGT
    CCCCCACAGC AGTATGGTTT TCCACCTGCC CCATTTGGCA CTCAGTGGAG TGGAACAGGT
    GGTCCAGCAC CGCAGCCACT TGGCCAGTTC CAACCTGTAG GAACAGCATC CTTGCAAAAT
    TTCAACATCA GCAATTTGCA GAAGTCCATC AGCAACCCCC CAGGTTCCAA TCTACGGACC
    ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu89190
    Clone ID Related Accession (Same CDS sequence) XM_030255355.1
    Accession Version XM_030255355.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGGGAATAC AGCCAACTGT TCCTTCTCAA CAGGCAGTAC AGTATTCACT TCCACAAGCA
    GCATCTTCCA GTGAAGGTAC TACTGCACAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGGGCT TCCCATCTCG ACTGCCACCA CAATACCCAG GAGATTCAAA TATTGCTCCC
    TCTTCCAACG TGGCTTCTGT TTGCATCCAT TCTACAGTCC TAGCCCCTCC TTCTATGCCG
    ACAGAAGCAT TGGCCACCCA AGGTTACTTT CCTACAGTAG TGCAGCCTTA TGTGGAATCA
    ACTCCTTTGG TTCCTATGGG CAGTGTAGGA GGCCAGGTTC AAGTGTCCCA ACCAGCAGTG
    AGTTTAACGC AACAACCCCC AACTACATCC TCCCAGCAAG CAGTTCTGGA GAGTACTCAG
    GGAGTCTCTC AGGCTGCTCC TCCAGAACAA ACTCCAATAA CACAGTCACA ACCTACTCAG
    CCTGTTCCAT TGGTTACCTC TGCAGACAGT GCACATTCAG ACGTTGCTTC TGGTATGAGT
    GATGGCAATG AGAATGCTCC ATCATCCAGC GGGAGGCATG AAGGGAGGAC AACAAAACGG
    CATTATAGAA AATCCGTAAG AAGTCGCTCT CGGCATGAAA AGACTTCTCG CCCAAAACTG
    AGGATTTTAA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT
    AATCGGAAGA TGGTTACATT TAAATTTGAC CTAGATGGTG ACAACCCTGA AGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCTTT TGTGGCTCAA
    GTGCGGGAAA TTATTGAAAA GGCTGATGAA ATGTTAAGTG AGGATGTCAG TGTGGAGCCT
    GAGGGTGACC AGGGATTGGA GAGTCTGCAA GGAAAGGATG ACTATGGCTT CCCAGGTTCT
    CAGAAATTAG AAGGAGAGTT CAAGCAACCA ATTGCTGTAT CTTCCATGCC ACAACAAATA
    GGTGTTCCTA CCAGTTCTTT AACGCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTAC CTGAAAGTAG GTTACGAGAA TCAAAAGTTT TCACCAGTGA CATATCAGAT
    CCAGTTGTGG CCTCTACATC TCAGGCCCCT GGAATGAACT TATCTCACTC TGCTTCATCC
    CTCAGTCTCC AGCAGGCTTT TTCTGAACTG AAACATGGTC AGATGACTGA AGGACCCAAT
    ACAGCACCTC CAAATTTTAA TCACATGGCG GGACCAACAT TTTCTCCCTT TTTGGCTAGC
    ATTGCTGGTG TCCAAACTGT AGCAGCATCC ACACCGTCAG TATCAGTCCC TATAACAAGC
    AGTCCTCTTA ATGACATTTC CACATCAGTG ATGCAGTCTG AGACTGCACT GCCCACTGAA
    AAAGGCATCG TGGGAGTTAC CACTACCAGC ACAGGTGTGG TAGCTTCAGG TGGTCTCACC
    ACAATGTCTG TGTCTGAGTC ACCAACATCT TCCAGTGCGG TTTCAAGTTC TACAGTACCT
    GCAGTTGTCA CTGTGTCCAC ACCATCCCAG CCAGTTCAGG CTTCCACATC TGGGAGCATT
    GCTTCAAGTA CAGGCAGTTT TCCTCCTGGA ACATTTTCTA CAACTACAGC TACTACAATG
    GGTAGTGTGG TGGCCCCAGA TGCTAAGCCT CCAACTGTAT TATTGCAACA GGTAGCAAGC
    AATACTGCTG GGGTTGCCAT AGTGACATCA GTCTCTACCA CCACTCCATT CCCAGGCATG
    GCTTCACAGC CATCCCTTCC ACTTAGTAGC AGCACCTCTG CTCCTACTCT AGCTGAAACA
    ATGGTGGTTA GTGCACACTC ACTAGACAAG GCATCTCACA GTAGTACAGC AGGATTGGGT
    TTGTCCTTTT GTGCGCCATC CTCTTCCTCC TCCTCTGGAA CAGCTGTGTC TACTTCTGTT
    TCTCAGCCTG GTATGGTTCA TCCTTTGGTC ATCTCATCAG CTGTAGTTTC TACACCTGGC
    CTCCCCCAAC CAGTAGTACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAATATCCCA
    CCCTTGGTAC AACCTGTTGT TAATGTGCCT GCTGTACAGC AAACACTCAT TCACAGTCAG
    CCTCAACCAG CTCTGCTTCC CAACCAGCCT CACACACACT GTCCTGAAAT GGATGCTGAT
    ACCCAATCCA AAGCTCCTGG GATTGATGAT ATAAAGACTC TGGAGGAAAA GCTGAGATCT
    CTCTTTAGTG AACACAGCTC ATCTGGAACT CAGCATGCTT CTGTCTCCCT AGAGACACCT
    TTAGTAGTAG AGACGACTGT CACACCGGGC ATCACTACCA CTGCTGTAGC ACCAAGTAAA
    CTCATGACTT CCACTACAAG TACTTGCTTA CCACCAACCA GTTTACCATT AGGAGCAGCT
    GGCATGCCAG TTATGCCAGT AGGCACACCT GGGCAGGTTT CTACCCCAGG CACACACGCT
    TCAGCCCCAG TCGGCACTGC AACAGGAGTA AAACCTGGAA CAACTCCCCC AAAGCCAACT
    AAGACTGTGG TGCCACCAGT GGGTACTGAA CTTTCAGCCG GTACTGTACC CTGTGAGCAG
    CTGCCACCTT TTCCTGGACC TTCTCTAATT CAGTCCCAGC AACCACTAGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTACAG TGGCACCTGC AGTTGGTCCT
    TTGTCTACGA TGTCTTCAAC AACAGTTACA GAAGCAGGGA CTCGGCTTCA AAAGGATGGC
    ACAGAAGGTC ATGTCACAGC AACTAGTTCA GGAGCTGGTG TTGTTAAGAT GGGGCGATTT
    CAGGTTTCAG TTACAATGGA TGATGCCCAG AAAGAACGTA AAAATAGGTC AGAAGATACA
    AAGTCGGTTC ATTTTGAATC TAGCACATCA GAGTCATCAG TTCTTTCAAG TAGTAGTCCA
    GAGAGTACCC TGGTAAAGCC AGAGCCTAAT GGAATTAGTA TTTCTGGCAT CTCCTTAGAT
    GTCCCAGACA GCACCCACAA AGCACCTACC CCAGAAGCAA AGTCAGATGC AGGACAGCCT
    ACCAAGGTGG GACGTTTTCA GGTGACAACT ACAGCCAACA AAGTGGGTCG TTTCTCTGTT
    TCAAGAACTG AGGACAAGGT CACAGAGCTC AAAAAAGAGG GACCAGTGAC CTCTCCTCCT
    TTCAGAGATT CAGAACAAAC TGTTATTCCT GCTGTGATAC CAAAGAAGGA GAAGCCTGAG
    CTGGCAGAAC CTTCCCATTT GAATGGGCCA TCTTCAGACC TGGAGGCTGC CTTTCTAAGT
    AGGGGTACAG AGGATGGCTC AGGTAGTCCA CACTCTCCCC CTCACCTGTG TTCAAAGAGC
    CTTCCCGTCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TCAACTCCTC ATACATGAGT
    AGTGACAATG AGTCCGACAT TGAAGATGAA GACTTAAGGT TAGAACTACG ACGACTACGA
    GAAAAACATC TTAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT
    TTGTATACTA AACTGGGCAA GGTTCCCCCT GCTGTCATTA TTCCCCCAGC TGCTCCTCTG
    TCGGGGAGAA GAAGGAGACC CACTAAAAGC AAAGGCAGCA AGTCTAGTCG CAGCAGCTCA
    TTGGGCAATA AAAGCCCACA GCTTTCAGGC AACCTGTCTG GTCAGAGTGG AACTTCAGTC
    TTACACCCCC AACAGACCCT CCATCCCGCA GGAAACACCC CCGAGACTGG GCACAACCAG
    CTGTTACAGC CTCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTTACCAGT
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGCTGTGC GAAGTTTAAC
    TGTGCATCTG AGCAGGTGAC ATTCAAACCT GGTGGCAGGA GGACCCGATT TCTGAGTACG
    CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGTC CTTGCAACCA GCTCTGTATG
    TGCCCTCCAG CTGAACCGAA ATCAGGGACC AGCAGCACAA ATACCGTTGG AGGAACAGTG
    AGTAGCCAGG CTGCCCAGGC TCAGCCTCCT GCTATGACTT CCAGTAGGAA GGGCACATTC
    ACAGATGACC TGCACAAGTT AGTAGACAAC TGGGCCCGAG ATGCCATGAA TCTTTCAGGC
    AGGAGAGGCA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT
    GCTCCTGGGC AGCTGTGTGT TCCCATGACC TCAAACCTGG GTGGCTCTAC CCCCATCTCT
    GCAGCATCTG CTACCTCTCT AGGTCACTTC ACCAAATCCA TGTGTCCCCC ACAGCAGTAT
    GGTTTTCCAC CTGCCCCATT TGGCACTCAG TGGAGTGGAA CAGGTGGTCC AGCACCGCAG
    CCACTTGGCC AGTTCCAACC TGTAGGAACA GCATCCTTGC AAAATTTCAA CATCAGCAAT
    TTGCAGAAGT CCATCAGCAA CCCCCCAGGT TCCAATCTAC GGACCACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030111215.1
    CDS885..7715
    Translation

    Target ORF information:

    RefSeq Version XM_030255355.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030255355.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGGGAATAC AGCCAACTGT TCCTTCTCAA CAGGCAGTAC AGTATTCACT TCCACAAGCA
    GCATCTTCCA GTGAAGGTAC TACTGCACAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGGGCT TCCCATCTCG ACTGCCACCA CAATACCCAG GAGATTCAAA TATTGCTCCC
    TCTTCCAACG TGGCTTCTGT TTGCATCCAT TCTACAGTCC TAGCCCCTCC TTCTATGCCG
    ACAGAAGCAT TGGCCACCCA AGGTTACTTT CCTACAGTAG TGCAGCCTTA TGTGGAATCA
    ACTCCTTTGG TTCCTATGGG CAGTGTAGGA GGCCAGGTTC AAGTGTCCCA ACCAGCAGTG
    AGTTTAACGC AACAACCCCC AACTACATCC TCCCAGCAAG CAGTTCTGGA GAGTACTCAG
    GGAGTCTCTC AGGCTGCTCC TCCAGAACAA ACTCCAATAA CACAGTCACA ACCTACTCAG
    CCTGTTCCAT TGGTTACCTC TGCAGACAGT GCACATTCAG ACGTTGCTTC TGGTATGAGT
    GATGGCAATG AGAATGCTCC ATCATCCAGC GGGAGGCATG AAGGGAGGAC AACAAAACGG
    CATTATAGAA AATCCGTAAG AAGTCGCTCT CGGCATGAAA AGACTTCTCG CCCAAAACTG
    AGGATTTTAA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT
    AATCGGAAGA TGGTTACATT TAAATTTGAC CTAGATGGTG ACAACCCTGA AGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCTTT TGTGGCTCAA
    GTGCGGGAAA TTATTGAAAA GGCTGATGAA ATGTTAAGTG AGGATGTCAG TGTGGAGCCT
    GAGGGTGACC AGGGATTGGA GAGTCTGCAA GGAAAGGATG ACTATGGCTT CCCAGGTTCT
    CAGAAATTAG AAGGAGAGTT CAAGCAACCA ATTGCTGTAT CTTCCATGCC ACAACAAATA
    GGTGTTCCTA CCAGTTCTTT AACGCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG
    AGTCCTGTAC CTGAAAGTAG GTTACGAGAA TCAAAAGTTT TCACCAGTGA CATATCAGAT
    CCAGTTGTGG CCTCTACATC TCAGGCCCCT GGAATGAACT TATCTCACTC TGCTTCATCC
    CTCAGTCTCC AGCAGGCTTT TTCTGAACTG AAACATGGTC AGATGACTGA AGGACCCAAT
    ACAGCACCTC CAAATTTTAA TCACATGGCG GGACCAACAT TTTCTCCCTT TTTGGCTAGC
    ATTGCTGGTG TCCAAACTGT AGCAGCATCC ACACCGTCAG TATCAGTCCC TATAACAAGC
    AGTCCTCTTA ATGACATTTC CACATCAGTG ATGCAGTCTG AGACTGCACT GCCCACTGAA
    AAAGGCATCG TGGGAGTTAC CACTACCAGC ACAGGTGTGG TAGCTTCAGG TGGTCTCACC
    ACAATGTCTG TGTCTGAGTC ACCAACATCT TCCAGTGCGG TTTCAAGTTC TACAGTACCT
    GCAGTTGTCA CTGTGTCCAC ACCATCCCAG CCAGTTCAGG CTTCCACATC TGGGAGCATT
    GCTTCAAGTA CAGGCAGTTT TCCTCCTGGA ACATTTTCTA CAACTACAGC TACTACAATG
    GGTAGTGTGG TGGCCCCAGA TGCTAAGCCT CCAACTGTAT TATTGCAACA GGTAGCAAGC
    AATACTGCTG GGGTTGCCAT AGTGACATCA GTCTCTACCA CCACTCCATT CCCAGGCATG
    GCTTCACAGC CATCCCTTCC ACTTAGTAGC AGCACCTCTG CTCCTACTCT AGCTGAAACA
    ATGGTGGTTA GTGCACACTC ACTAGACAAG GCATCTCACA GTAGTACAGC AGGATTGGGT
    TTGTCCTTTT GTGCGCCATC CTCTTCCTCC TCCTCTGGAA CAGCTGTGTC TACTTCTGTT
    TCTCAGCCTG GTATGGTTCA TCCTTTGGTC ATCTCATCAG CTGTAGTTTC TACACCTGGC
    CTCCCCCAAC CAGTAGTACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAATATCCCA
    CCCTTGGTAC AACCTGTTGT TAATGTGCCT GCTGTACAGC AAACACTCAT TCACAGTCAG
    CCTCAACCAG CTCTGCTTCC CAACCAGCCT CACACACACT GTCCTGAAAT GGATGCTGAT
    ACCCAATCCA AAGCTCCTGG GATTGATGAT ATAAAGACTC TGGAGGAAAA GCTGAGATCT
    CTCTTTAGTG AACACAGCTC ATCTGGAACT CAGCATGCTT CTGTCTCCCT AGAGACACCT
    TTAGTAGTAG AGACGACTGT CACACCGGGC ATCACTACCA CTGCTGTAGC ACCAAGTAAA
    CTCATGACTT CCACTACAAG TACTTGCTTA CCACCAACCA GTTTACCATT AGGAGCAGCT
    GGCATGCCAG TTATGCCAGT AGGCACACCT GGGCAGGTTT CTACCCCAGG CACACACGCT
    TCAGCCCCAG TCGGCACTGC AACAGGAGTA AAACCTGGAA CAACTCCCCC AAAGCCAACT
    AAGACTGTGG TGCCACCAGT GGGTACTGAA CTTTCAGCCG GTACTGTACC CTGTGAGCAG
    CTGCCACCTT TTCCTGGACC TTCTCTAATT CAGTCCCAGC AACCACTAGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTACAG TGGCACCTGC AGTTGGTCCT
    TTGTCTACGA TGTCTTCAAC AACAGTTACA GAAGCAGGGA CTCGGCTTCA AAAGGATGGC
    ACAGAAGGTC ATGTCACAGC AACTAGTTCA GGAGCTGGTG TTGTTAAGAT GGGGCGATTT
    CAGGTTTCAG TTACAATGGA TGATGCCCAG AAAGAACGTA AAAATAGGTC AGAAGATACA
    AAGTCGGTTC ATTTTGAATC TAGCACATCA GAGTCATCAG TTCTTTCAAG TAGTAGTCCA
    GAGAGTACCC TGGTAAAGCC AGAGCCTAAT GGAATTAGTA TTTCTGGCAT CTCCTTAGAT
    GTCCCAGACA GCACCCACAA AGCACCTACC CCAGAAGCAA AGTCAGATGC AGGACAGCCT
    ACCAAGGTGG GACGTTTTCA GGTGACAACT ACAGCCAACA AAGTGGGTCG TTTCTCTGTT
    TCAAGAACTG AGGACAAGGT CACAGAGCTC AAAAAAGAGG GACCAGTGAC CTCTCCTCCT
    TTCAGAGATT CAGAACAAAC TGTTATTCCT GCTGTGATAC CAAAGAAGGA GAAGCCTGAG
    CTGGCAGAAC CTTCCCATTT GAATGGGCCA TCTTCAGACC TGGAGGCTGC CTTTCTAAGT
    AGGGGTACAG AGGATGGCTC AGGTAGTCCA CACTCTCCCC CTCACCTGTG TTCAAAGAGC
    CTTCCCGTCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TCAACTCCTC ATACATGAGT
    AGTGACAATG AGTCCGACAT TGAAGATGAA GACTTAAGGT TAGAACTACG ACGACTACGA
    GAAAAACATC TTAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT
    TTGTATACTA AACTGGGCAA GGTTCCCCCT GCTGTCATTA TTCCCCCAGC TGCTCCTCTG
    TCGGGGAGAA GAAGGAGACC CACTAAAAGC AAAGGCAGCA AGTCTAGTCG CAGCAGCTCA
    TTGGGCAATA AAAGCCCACA GCTTTCAGGC AACCTGTCTG GTCAGAGTGG AACTTCAGTC
    TTACACCCCC AACAGACCCT CCATCCCGCA GGAAACACCC CCGAGACTGG GCACAACCAG
    CTGTTACAGC CTCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTTACCAGT
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGCTGTGC GAAGTTTAAC
    TGTGCATCTG AGCAGGTGAC ATTCAAACCT GGTGGCAGGA GGACCCGATT TCTGAGTACG
    CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGTC CTTGCAACCA GCTCTGTATG
    TGCCCTCCAG CTGAACCGAA ATCAGGGACC AGCAGCACAA ATACCGTTGG AGGAACAGTG
    AGTAGCCAGG CTGCCCAGGC TCAGCCTCCT GCTATGACTT CCAGTAGGAA GGGCACATTC
    ACAGATGACC TGCACAAGTT AGTAGACAAC TGGGCCCGAG ATGCCATGAA TCTTTCAGGC
    AGGAGAGGCA GCAAAGGACA CATGAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTCTCT
    GCTCCTGGGC AGCTGTGTGT TCCCATGACC TCAAACCTGG GTGGCTCTAC CCCCATCTCT
    GCAGCATCTG CTACCTCTCT AGGTCACTTC ACCAAATCCA TGTGTCCCCC ACAGCAGTAT
    GGTTTTCCAC CTGCCCCATT TGGCACTCAG TGGAGTGGAA CAGGTGGTCC AGCACCGCAG
    CCACTTGGCC AGTTCCAACC TGTAGGAACA GCATCCTTGC AAAATTTCAA CATCAGCAAT
    TTGCAGAAGT CCATCAGCAA CCCCCCAGGT TCCAATCTAC GGACCACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15602
    Clone ID Related Accession (Same CDS sequence) NM_198703.3 , XM_017321549.1
    Accession Version NM_198703.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133738.1, AY309076.1, BC171955.1 and BC056761.1. On May 29, 2010 this sequence version replaced NM_198703.2. Transcript Variant: This variant (1) has multiple differences in the coding region but maintains the reading frame compared to variant 5. The resulting protein (isoform 1) is shorter but has the same N- and C-termini compared to isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY309076.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAATACCGT TGGAGGAACA
    GTGAGTAGCC AGGCTGCCCA GGCTCAGCCT CCTGCTATGA CTTCCAGTAG GAAGGGCACA
    TTCACAGATG ACCTGCACAA GTTAGTAGAC AACTGGGCCC GAGATGCCAT GAATCTTTCA
    GGCAGGAGAG GCAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC
    TCTGCTCCTG GGCAGCTGTG TGTTCCCATG ACCTCAAACC TGGGTGGCTC TACCCCCATC
    TCTGCAGCAT CTGCTACCTC TCTAGGTCAC TTCACCAAAT CCATGTGTCC CCCACAGCAG
    TATGGTTTTC CACCTGCCCC ATTTGGCACT CAGTGGAGTG GAACAGGTGG TCCAGCACCG
    CAGCCACTTG GCCAGTTCCA ACCTGTAGGA ACAGCATCCT TGCAAAATTT CAACATCAGC
    AATTTGCAGA AGTCCATCAG CAACCCCCCA GGTTCCAATC TACGGACCAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_941992.2
    CDS1024..8157
    Translation

    Target ORF information:

    RefSeq Version NM_198703.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_198703.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAATACCGT TGGAGGAACA
    GTGAGTAGCC AGGCTGCCCA GGCTCAGCCT CCTGCTATGA CTTCCAGTAG GAAGGGCACA
    TTCACAGATG ACCTGCACAA GTTAGTAGAC AACTGGGCCC GAGATGCCAT GAATCTTTCA
    GGCAGGAGAG GCAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC
    TCTGCTCCTG GGCAGCTGTG TGTTCCCATG ACCTCAAACC TGGGTGGCTC TACCCCCATC
    TCTGCAGCAT CTGCTACCTC TCTAGGTCAC TTCACCAAAT CCATGTGTCC CCCACAGCAG
    TATGGTTTTC CACCTGCCCC ATTTGGCACT CAGTGGAGTG GAACAGGTGG TCCAGCACCG
    CAGCCACTTG GCCAGTTCCA ACCTGTAGGA ACAGCATCCT TGCAAAATTT CAACATCAGC
    AATTTGCAGA AGTCCATCAG CAACCCCCCA GGTTCCAATC TACGGACCAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15715
    Clone ID Related Accession (Same CDS sequence) XM_017321557.1 , NM_001185020.1
    Accession Version NM_001185020.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133738.1, BC138445.1 and BC056761.1. On or before Nov 23, 2011 this sequence version replaced XM_003086528.1, XM_003084646.1, NR_028257.1. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame compared to variant 5. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGA GTACTCAGGG AGTCTCTCAG GCTGCTCCTC CAGAACAAAC TCCAATAACA
    CAGTCACAAC CTACTCAGCC TGTTCCATTG GTTACCTCTG CAGACAGTGC ACATTCAGAC
    GTTGCTTCTG GTATGAGTGA TGGCAATGAG AATGCTCCAT CATCCAGCGG GAGGCATGAA
    GGGAGGACAA CAAAACGGCA TTATAGAAAA TCCGTAAGAA GTCGCTCTCG GCATGAAAAG
    ACTTCTCGCC CAAAACTGAG GATTTTAAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TCGGAAGATG GTTACATTTA AATTTGACCT AGATGGTGAC
    AACCCTGAAG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCTTTTG TGGCTCAAGT GCGGGAAATT ATTGAAAAGG CTGATGAAAT GTTAAGTGAG
    GATGTCAGTG TGGAGCCTGA GGGTGACCAG GGATTGGAGA GTCTGCAAGG AAAGGATGAC
    TATGGCTTCC CAGGTTCTCA GAAATTAGAA GGAGAGTTCA AGCAACCAAT TGCTGTATCT
    TCCATGCCAC AACAAATAGG TGTTCCTACC AGTTCTTTAA CGCAAGTTGT TCATTCTGCT
    GGAAGACGGT TTATAGTGAG TCCTGTACCT GAAAGTAGGT TACGAGAATC AAAAGTTTTC
    ACCAGTGACA TATCAGATCC AGTTGTGGCC TCTACATCTC AGGCCCCTGG AATGAACTTA
    TCTCACTCTG CTTCATCCCT CAGTCTCCAG CAGGCTTTTT CTGAACTGAA ACATGGTCAG
    ATGACTGAAG GACCCAATAC AGCACCTCCA AATTTTAATC ACATGGCGGG ACCAACATTT
    TCTCCCTTTT TGGCTAGCAT TGCTGGTGTC CAAACTGTAG CAGCATCCAC ACCGTCAGTA
    TCAGTCCCTA TAACAAGCAG TCCTCTTAAT GACATTTCCA CATCAGTGAT GCAGTCTGAG
    ACTGCACTGC CCACTGAAAA AGGCATCGTG GGAGTTACCA CTACCAGCAC AGGTGTGGTA
    GCTTCAGGTG GTCTCACCAC AATGTCTGTG TCTGAGTCAC CAACATCTTC CAGTGCGGTT
    TCAAGTTCTA CAGTACCTGC AGTTGTCACT GTGTCCACAC CATCCCAGCC AGTTCAGGCT
    TCCACATCTG GGAGCATTGC TTCAAGTACA GGCAGTTTTC CTCCTGGAAC ATTTTCTACA
    ACTACAGCTA CTACAATGGG TAGTGTGGTG GCCCCAGATG CTAAGCCTCC AACTGTATTA
    TTGCAACAGG TAGCAAGCAA TACTGCTGGG GTTGCCATAG TGACATCAGT CTCTACCACC
    ACTCCATTCC CAGGCATGGC TTCACAGCCA TCCCTTCCAC TTAGTAGCAG CACCTCTGCT
    CCTACTCTAG CTGAAACAAT GGTGGTTAGT GCACACTCAC TAGACAAGGC ATCTCACAGT
    AGTACAGCAG GATTGGGTTT GTCCTTTTGT GCGCCATCCT CTTCCTCCTC CTCTGGAACA
    GCTGTGTCTA CTTCTGTTTC TCAGCCTGGT ATGGTTCATC CTTTGGTCAT CTCATCAGCT
    GTAGTTTCTA CACCTGGCCT CCCCCAACCA GTAGTACCTA CTTCTACACC TTTATTACCC
    CAAGTACCTA ATATCCCACC CTTGGTACAA CCTGTTGTTA ATGTGCCTGC TGTACAGCAA
    ACACTCATTC ACAGTCAGCC TCAACCAGCT CTGCTTCCCA ACCAGCCTCA CACACACTGT
    CCTGAAATGG ATGCTGATAC CCAATCCAAA GCTCCTGGGA TTGATGATAT AAAGACTCTG
    GAGGAAAAGC TGAGATCTCT CTTTAGTGAA CACAGCTCAT CTGGAACTCA GCATGCTTCT
    GTCTCCCTAG AGACACCTTT AGTAGTAGAG ACGACTGTCA CACCGGGCAT CACTACCACT
    GCTGTAGCAC CAAGTAAACT CATGACTTCC ACTACAAGTA CTTGCTTACC ACCAACCAGT
    TTACCATTAG GAGCAGCTGG CATGCCAGTT ATGCCAGTAG GCACACCTGG GCAGGTTTCT
    ACCCCAGGCA CACACGCTTC AGCCCCAGTC GGCACTGCAA CAGGAGTAAA ACCTGGAACA
    ACTCCCCCAA AGCCAACTAA GACTGTGGTG CCACCAGTGG GTACTGAACT TTCAGCCGGT
    ACTGTACCCT GTGAGCAGCT GCCACCTTTT CCTGGACCTT CTCTAATTCA GTCCCAGCAA
    CCACTAGAAG ATCTTGATGC TCAGTTGAGA AGAACACTTA GTCCAGAGAC TATTACAGTG
    GCACCTGCAG TTGGTCCTTT GTCTACGATG TCTTCAACAA CAGTTACAGA AGCAGGGACT
    CGGCTTCAAA AGGATGGCAC AGAAGGTCAT GTCACAGCAA CTAGTTCAGG AGCTGGTGTT
    GTTAAGATGG GGCGATTTCA GGTTTCAGTT ACAATGGATG ATGCCCAGAA AGAACGTAAA
    AATAGGTCAG AAGATACAAA GTCGGTTCAT TTTGAATCTA GCACATCAGA GTCATCAGTT
    CTTTCAAGTA GTAGTCCAGA GAGTACCCTG GTAAAGCCAG AGCCTAATGG AATTAGTATT
    TCTGGCATCT CCTTAGATGT CCCAGACAGC ACCCACAAAG CACCTACCCC AGAAGCAAAG
    TCAGATGCAG GACAGCCTAC CAAGGTGGGA CGTTTTCAGG TGACAACTAC AGCCAACAAA
    GTGGGTCGTT TCTCTGTTTC AAGAACTGAG GACAAGGTCA CAGAGCTCAA AAAAGAGGGA
    CCAGTGACCT CTCCTCCTTT CAGAGATTCA GAACAAACTG TTATTCCTGC TGTGATACCA
    AAGAAGGAGA AGCCTGAGCT GGCAGAACCT TCCCATTTGA ATGGGCCATC TTCAGACCTG
    GAGGCTGCCT TTCTAAGTAG GGGTACAGAG GATGGCTCAG GTAGTCCACA CTCTCCCCCT
    CACCTGTGTT CAAAGAGCCT TCCCGTCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTC
    AACTCCTCAT ACATGAGTAG TGACAATGAG TCCGACATTG AAGATGAAGA CTTAAGGTTA
    GAACTACGAC GACTACGAGA AAAACATCTT AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACTAAA CTGGGCAAGG TTCCCCCTGC TGTCATTATT
    CCCCCAGCTG CTCCTCTGTC GGGGAGAAGA AGGAGACCCA CTAAAAGCAA AGGCAGCAAG
    TCTAGTCGCA GCAGCTCATT GGGCAATAAA AGCCCACAGC TTTCAGGCAA CCTGTCTGGT
    CAGAGTGGAA CTTCAGTCTT ACACCCCCAA CAGACCCTCC ATCCCGCAGG AAACACCCCC
    GAGACTGGGC ACAACCAGCT GTTACAGCCT CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TTACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGGACCAGCA GCACAAATAC CGTTGGAGGA ACAGTGAGTA GCCAGGCTGC CCAGGCTCAG
    CCTCCTGCTA TGACTTCCAG TAGGAAGGGC ACATTCACAG ATGACCTGCA CAAGTTAGTA
    GACAACTGGG CCCGAGATGC CATGAATCTT TCAGGCAGGA GAGGCAGCAA AGGACACATG
    AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCTC CTGGGCAGCT GTGTGTTCCC
    ATGACCTCAA ACCTGGGTGG CTCTACCCCC ATCTCTGCAG CATCTGCTAC CTCTCTAGGT
    CACTTCACCA AATCCATGTG TCCCCCACAG CAGTATGGTT TTCCACCTGC CCCATTTGGC
    ACTCAGTGGA GTGGAACAGG TGGTCCAGCA CCGCAGCCAC TTGGCCAGTT CCAACCTGTA
    GGAACAGCAT CCTTGCAAAA TTTCAACATC AGCAATTTGC AGAAGTCCAT CAGCAACCCC
    CCAGGTTCCA ATCTACGGAC CACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171949.1
    CDS1024..7410
    Translation

    Target ORF information:

    RefSeq Version NM_001185020.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001185020.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGA GTACTCAGGG AGTCTCTCAG GCTGCTCCTC CAGAACAAAC TCCAATAACA
    CAGTCACAAC CTACTCAGCC TGTTCCATTG GTTACCTCTG CAGACAGTGC ACATTCAGAC
    GTTGCTTCTG GTATGAGTGA TGGCAATGAG AATGCTCCAT CATCCAGCGG GAGGCATGAA
    GGGAGGACAA CAAAACGGCA TTATAGAAAA TCCGTAAGAA GTCGCTCTCG GCATGAAAAG
    ACTTCTCGCC CAAAACTGAG GATTTTAAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TCGGAAGATG GTTACATTTA AATTTGACCT AGATGGTGAC
    AACCCTGAAG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCTTTTG TGGCTCAAGT GCGGGAAATT ATTGAAAAGG CTGATGAAAT GTTAAGTGAG
    GATGTCAGTG TGGAGCCTGA GGGTGACCAG GGATTGGAGA GTCTGCAAGG AAAGGATGAC
    TATGGCTTCC CAGGTTCTCA GAAATTAGAA GGAGAGTTCA AGCAACCAAT TGCTGTATCT
    TCCATGCCAC AACAAATAGG TGTTCCTACC AGTTCTTTAA CGCAAGTTGT TCATTCTGCT
    GGAAGACGGT TTATAGTGAG TCCTGTACCT GAAAGTAGGT TACGAGAATC AAAAGTTTTC
    ACCAGTGACA TATCAGATCC AGTTGTGGCC TCTACATCTC AGGCCCCTGG AATGAACTTA
    TCTCACTCTG CTTCATCCCT CAGTCTCCAG CAGGCTTTTT CTGAACTGAA ACATGGTCAG
    ATGACTGAAG GACCCAATAC AGCACCTCCA AATTTTAATC ACATGGCGGG ACCAACATTT
    TCTCCCTTTT TGGCTAGCAT TGCTGGTGTC CAAACTGTAG CAGCATCCAC ACCGTCAGTA
    TCAGTCCCTA TAACAAGCAG TCCTCTTAAT GACATTTCCA CATCAGTGAT GCAGTCTGAG
    ACTGCACTGC CCACTGAAAA AGGCATCGTG GGAGTTACCA CTACCAGCAC AGGTGTGGTA
    GCTTCAGGTG GTCTCACCAC AATGTCTGTG TCTGAGTCAC CAACATCTTC CAGTGCGGTT
    TCAAGTTCTA CAGTACCTGC AGTTGTCACT GTGTCCACAC CATCCCAGCC AGTTCAGGCT
    TCCACATCTG GGAGCATTGC TTCAAGTACA GGCAGTTTTC CTCCTGGAAC ATTTTCTACA
    ACTACAGCTA CTACAATGGG TAGTGTGGTG GCCCCAGATG CTAAGCCTCC AACTGTATTA
    TTGCAACAGG TAGCAAGCAA TACTGCTGGG GTTGCCATAG TGACATCAGT CTCTACCACC
    ACTCCATTCC CAGGCATGGC TTCACAGCCA TCCCTTCCAC TTAGTAGCAG CACCTCTGCT
    CCTACTCTAG CTGAAACAAT GGTGGTTAGT GCACACTCAC TAGACAAGGC ATCTCACAGT
    AGTACAGCAG GATTGGGTTT GTCCTTTTGT GCGCCATCCT CTTCCTCCTC CTCTGGAACA
    GCTGTGTCTA CTTCTGTTTC TCAGCCTGGT ATGGTTCATC CTTTGGTCAT CTCATCAGCT
    GTAGTTTCTA CACCTGGCCT CCCCCAACCA GTAGTACCTA CTTCTACACC TTTATTACCC
    CAAGTACCTA ATATCCCACC CTTGGTACAA CCTGTTGTTA ATGTGCCTGC TGTACAGCAA
    ACACTCATTC ACAGTCAGCC TCAACCAGCT CTGCTTCCCA ACCAGCCTCA CACACACTGT
    CCTGAAATGG ATGCTGATAC CCAATCCAAA GCTCCTGGGA TTGATGATAT AAAGACTCTG
    GAGGAAAAGC TGAGATCTCT CTTTAGTGAA CACAGCTCAT CTGGAACTCA GCATGCTTCT
    GTCTCCCTAG AGACACCTTT AGTAGTAGAG ACGACTGTCA CACCGGGCAT CACTACCACT
    GCTGTAGCAC CAAGTAAACT CATGACTTCC ACTACAAGTA CTTGCTTACC ACCAACCAGT
    TTACCATTAG GAGCAGCTGG CATGCCAGTT ATGCCAGTAG GCACACCTGG GCAGGTTTCT
    ACCCCAGGCA CACACGCTTC AGCCCCAGTC GGCACTGCAA CAGGAGTAAA ACCTGGAACA
    ACTCCCCCAA AGCCAACTAA GACTGTGGTG CCACCAGTGG GTACTGAACT TTCAGCCGGT
    ACTGTACCCT GTGAGCAGCT GCCACCTTTT CCTGGACCTT CTCTAATTCA GTCCCAGCAA
    CCACTAGAAG ATCTTGATGC TCAGTTGAGA AGAACACTTA GTCCAGAGAC TATTACAGTG
    GCACCTGCAG TTGGTCCTTT GTCTACGATG TCTTCAACAA CAGTTACAGA AGCAGGGACT
    CGGCTTCAAA AGGATGGCAC AGAAGGTCAT GTCACAGCAA CTAGTTCAGG AGCTGGTGTT
    GTTAAGATGG GGCGATTTCA GGTTTCAGTT ACAATGGATG ATGCCCAGAA AGAACGTAAA
    AATAGGTCAG AAGATACAAA GTCGGTTCAT TTTGAATCTA GCACATCAGA GTCATCAGTT
    CTTTCAAGTA GTAGTCCAGA GAGTACCCTG GTAAAGCCAG AGCCTAATGG AATTAGTATT
    TCTGGCATCT CCTTAGATGT CCCAGACAGC ACCCACAAAG CACCTACCCC AGAAGCAAAG
    TCAGATGCAG GACAGCCTAC CAAGGTGGGA CGTTTTCAGG TGACAACTAC AGCCAACAAA
    GTGGGTCGTT TCTCTGTTTC AAGAACTGAG GACAAGGTCA CAGAGCTCAA AAAAGAGGGA
    CCAGTGACCT CTCCTCCTTT CAGAGATTCA GAACAAACTG TTATTCCTGC TGTGATACCA
    AAGAAGGAGA AGCCTGAGCT GGCAGAACCT TCCCATTTGA ATGGGCCATC TTCAGACCTG
    GAGGCTGCCT TTCTAAGTAG GGGTACAGAG GATGGCTCAG GTAGTCCACA CTCTCCCCCT
    CACCTGTGTT CAAAGAGCCT TCCCGTCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTC
    AACTCCTCAT ACATGAGTAG TGACAATGAG TCCGACATTG AAGATGAAGA CTTAAGGTTA
    GAACTACGAC GACTACGAGA AAAACATCTT AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACTAAA CTGGGCAAGG TTCCCCCTGC TGTCATTATT
    CCCCCAGCTG CTCCTCTGTC GGGGAGAAGA AGGAGACCCA CTAAAAGCAA AGGCAGCAAG
    TCTAGTCGCA GCAGCTCATT GGGCAATAAA AGCCCACAGC TTTCAGGCAA CCTGTCTGGT
    CAGAGTGGAA CTTCAGTCTT ACACCCCCAA CAGACCCTCC ATCCCGCAGG AAACACCCCC
    GAGACTGGGC ACAACCAGCT GTTACAGCCT CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TTACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGGACCAGCA GCACAAATAC CGTTGGAGGA ACAGTGAGTA GCCAGGCTGC CCAGGCTCAG
    CCTCCTGCTA TGACTTCCAG TAGGAAGGGC ACATTCACAG ATGACCTGCA CAAGTTAGTA
    GACAACTGGG CCCGAGATGC CATGAATCTT TCAGGCAGGA GAGGCAGCAA AGGACACATG
    AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCTC CTGGGCAGCT GTGTGTTCCC
    ATGACCTCAA ACCTGGGTGG CTCTACCCCC ATCTCTGCAG CATCTGCTAC CTCTCTAGGT
    CACTTCACCA AATCCATGTG TCCCCCACAG CAGTATGGTT TTCCACCTGC CCCATTTGGC
    ACTCAGTGGA GTGGAACAGG TGGTCCAGCA CCGCAGCCAC TTGGCCAGTT CCAACCTGTA
    GGAACAGCAT CCTTGCAAAA TTTCAACATC AGCAATTTGC AGAAGTCCAT CAGCAACCCC
    CCAGGTTCCA ATCTACGGAC CACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15743
    Clone ID Related Accession (Same CDS sequence) NM_001185021.1 , XM_017321554.1
    Accession Version NM_001185021.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133738.1, BC145282.1 and BC056761.1. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame compared to variant 5. The resulting protein (isoform 3) is shorter but has the same N- and C-termini compared to isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145282.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGG GCTTCCCATC TCGACTGCCA CCACAATACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTAGCCCC TCCTTCTATG
    CCGACAGAAG CATTGGCCAC CCAAGGTTAC TTTCCTACAG TAGTGCAGCC TTATGTGGAA
    TCAACTCCTT TGGTTCCTAT GGGCAGTGTA GGAGGCCAGG TTCAAGTGTC CCAACCAGCA
    GTGAGTTTAA CGCAACAACC CCCAACTACA TCCTCCCAGC AAGCAGTTCT GGAGAGTACT
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAGCCTGTTC CATTGGTTAC CTCTGCAGAC AGTGCACATT CAGACGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCATCATCC AGCGGGAGGC ATGAAGGGAG GACAACAAAA
    CGGCATTATA GAAAATCCGT AAGAAGTCGC TCTCGGCATG AAAAGACTTC TCGCCCAAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTAGAGACT
    CATAATCGGA AGATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GTGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTG CAAGGAAAGG ATGACTATGG CTTCCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACGCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TACCTGAAAG TAGGTTACGA GAATCAAAAG TTTTCACCAG TGACATATCA
    GATCCAGTTG TGGCCTCTAC ATCTCAGGCC CCTGGAATGA ACTTATCTCA CTCTGCTTCA
    TCCCTCAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACATG GCGGGACCAA CATTTTCTCC CTTTTTGGCT
    AGCATTGCTG GTGTCCAAAC TGTAGCAGCA TCCACACCGT CAGTATCAGT CCCTATAACA
    AGCAGTCCTC TTAATGACAT TTCCACATCA GTGATGCAGT CTGAGACTGC ACTGCCCACT
    GAAAAAGGCA TCGTGGGAGT TACCACTACC AGCACAGGTG TGGTAGCTTC AGGTGGTCTC
    ACCACAATGT CTGTGTCTGA GTCACCAACA TCTTCCAGTG CGGTTTCAAG TTCTACAGTA
    CCTGCAGTTG TCACTGTGTC CACACCATCC CAGCCAGTTC AGGCTTCCAC ATCTGGGAGC
    ATTGCTTCAA GTACAGGCAG TTTTCCTCCT GGAACATTTT CTACAACTAC AGCTACTACA
    ATGGGTAGTG TGGTGGCCCC AGATGCTAAG CCTCCAACTG TATTATTGCA ACAGGTAGCA
    AGCAATACTG CTGGGGTTGC CATAGTGACA TCAGTCTCTA CCACCACTCC ATTCCCAGGC
    ATGGCTTCAC AGCCATCCCT TCCACTTAGT AGCAGCACCT CTGCTCCTAC TCTAGCTGAA
    ACAATGGTGG TTAGTGCACA CTCACTAGAC AAGGCATCTC ACAGTAGTAC AGCAGGATTG
    GGTTTGTCCT TTTGTGCGCC ATCCTCTTCC TCCTCCTCTG GAACAGCTGT GTCTACTTCT
    GTTTCTCAGC CTGGTATGGT TCATCCTTTG GTCATCTCAT CAGCTGTAGT TTCTACACCT
    GGCCTCCCCC AACCAGTAGT ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAATATC
    CCACCCTTGG TACAACCTGT TGTTAATGTG CCTGCTGTAC AGCAAACACT CATTCACAGT
    CAGCCTCAAC CAGCTCTGCT TCCCAACCAG CCTCACACAC ACTGTCCTGA AATGGATGCT
    GATACCCAAT CCAAAGCTCC TGGGATTGAT GATATAAAGA CTCTGGAGGA AAAGCTGAGA
    TCTCTCTTTA GTGAACACAG CTCATCTGGA ACTCAGCATG CTTCTGTCTC CCTAGAGACA
    CCTTTAGTAG TAGAGACGAC TGTCACACCG GGCATCACTA CCACTGCTGT AGCACCAAGT
    AAACTCATGA CTTCCACTAC AAGTACTTGC TTACCACCAA CCAGTTTACC ATTAGGAGCA
    GCTGGCATGC CAGTTATGCC AGTAGGCACA CCTGGGCAGG TTTCTACCCC AGGCACACAC
    GCTTCAGCCC CAGTCGGCAC TGCAACAGGA GTAAAACCTG GAACAACTCC CCCAAAGCCA
    ACTAAGACTG TGGTGCCACC AGTGGGTACT GAACTTTCAG CCGGTACTGT ACCCTGTGAG
    CAGCTGCCAC CTTTTCCTGG ACCTTCTCTA ATTCAGCCTT TGTCTACGAT GTCTTCAACA
    ACAGTTACAG AAGCAGGGAC TCGGCTTCAA AAGGATGGCA CAGAAGGTCA TGTCACAGCA
    ACTAGTTCAG GAGCTGGTGT TGTTAAGATG GGGCGATTTC AGGTTTCAGT TACAATGGAT
    GATGCCCAGA AAGAACGTAA AAATAGGTCA GAAGATACAA AGTCGGTTCA TTTTGAATCT
    AGCACATCAG AGTCATCAGT TCTTTCAAGT AGTAGTCCAG AGAGTACCCT GGTAAAGCCA
    GAGCCTAATG GAATTAGTAT TTCTGGCATC TCCTTAGATG TCCCAGACAG CACCCACAAA
    GCACCTACCC CAGAAGCAAA GTCAGATGCA GGACAGCCTA CCAAGGTGGG ACGTTTTCAG
    GTGACAACTA CAGCCAACAA AGTGGGTCGT TTCTCTGTTT CAAGAACTGA GGACAAGGTC
    ACAGAGCTCA AAAAAGAGGG ACCAGTGACC TCTCCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGTGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATTTG
    AATGGGCCAT CTTCAGACCT GGAGGCTGCC TTTCTAAGTA GGGGTACAGA GGATGGCTCA
    GGTAGTCCAC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCGTCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCA TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTACGA CGACTACGAG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACTAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CGGGGAGAAG AAGGAGACCC
    ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC AGCAGCTCAT TGGGCAATAA AAGCCCACAG
    CTTTCAGGCA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TACACCCCCA ACAGACCCTC
    CATCCCGCAG GAAACACCCC CGAGACTGGG CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGGACCAGC AGCACAAATA CCGTTGGAGG AACAGTGAGT
    AGCCAGGCTG CCCAGGCTCA GCCTCCTGCT ATGACTTCCA GTAGGAAGGG CACATTCACA
    GATGACCTGC ACAAGTTAGT AGACAACTGG GCCCGAGATG CCATGAATCT TTCAGGCAGG
    AGAGGCAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT
    CCTGGGCAGC TGTGTGTTCC CATGACCTCA AACCTGGGTG GCTCTACCCC CATCTCTGCA
    GCATCTGCTA CCTCTCTAGG TCACTTCACC AAATCCATGT GTCCCCCACA GCAGTATGGT
    TTTCCACCTG CCCCATTTGG CACTCAGTGG AGTGGAACAG GTGGTCCAGC ACCGCAGCCA
    CTTGGCCAGT TCCAACCTGT AGGAACAGCA TCCTTGCAAA ATTTCAACAT CAGCAATTTG
    CAGAAGTCCA TCAGCAACCC CCCAGGTTCC AATCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171950.1
    CDS1024..7611
    Translation

    Target ORF information:

    RefSeq Version NM_001185021.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001185021.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGG GCTTCCCATC TCGACTGCCA CCACAATACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTAGCCCC TCCTTCTATG
    CCGACAGAAG CATTGGCCAC CCAAGGTTAC TTTCCTACAG TAGTGCAGCC TTATGTGGAA
    TCAACTCCTT TGGTTCCTAT GGGCAGTGTA GGAGGCCAGG TTCAAGTGTC CCAACCAGCA
    GTGAGTTTAA CGCAACAACC CCCAACTACA TCCTCCCAGC AAGCAGTTCT GGAGAGTACT
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAGCCTGTTC CATTGGTTAC CTCTGCAGAC AGTGCACATT CAGACGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCATCATCC AGCGGGAGGC ATGAAGGGAG GACAACAAAA
    CGGCATTATA GAAAATCCGT AAGAAGTCGC TCTCGGCATG AAAAGACTTC TCGCCCAAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTAGAGACT
    CATAATCGGA AGATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GTGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTG CAAGGAAAGG ATGACTATGG CTTCCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACGCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TACCTGAAAG TAGGTTACGA GAATCAAAAG TTTTCACCAG TGACATATCA
    GATCCAGTTG TGGCCTCTAC ATCTCAGGCC CCTGGAATGA ACTTATCTCA CTCTGCTTCA
    TCCCTCAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACATG GCGGGACCAA CATTTTCTCC CTTTTTGGCT
    AGCATTGCTG GTGTCCAAAC TGTAGCAGCA TCCACACCGT CAGTATCAGT CCCTATAACA
    AGCAGTCCTC TTAATGACAT TTCCACATCA GTGATGCAGT CTGAGACTGC ACTGCCCACT
    GAAAAAGGCA TCGTGGGAGT TACCACTACC AGCACAGGTG TGGTAGCTTC AGGTGGTCTC
    ACCACAATGT CTGTGTCTGA GTCACCAACA TCTTCCAGTG CGGTTTCAAG TTCTACAGTA
    CCTGCAGTTG TCACTGTGTC CACACCATCC CAGCCAGTTC AGGCTTCCAC ATCTGGGAGC
    ATTGCTTCAA GTACAGGCAG TTTTCCTCCT GGAACATTTT CTACAACTAC AGCTACTACA
    ATGGGTAGTG TGGTGGCCCC AGATGCTAAG CCTCCAACTG TATTATTGCA ACAGGTAGCA
    AGCAATACTG CTGGGGTTGC CATAGTGACA TCAGTCTCTA CCACCACTCC ATTCCCAGGC
    ATGGCTTCAC AGCCATCCCT TCCACTTAGT AGCAGCACCT CTGCTCCTAC TCTAGCTGAA
    ACAATGGTGG TTAGTGCACA CTCACTAGAC AAGGCATCTC ACAGTAGTAC AGCAGGATTG
    GGTTTGTCCT TTTGTGCGCC ATCCTCTTCC TCCTCCTCTG GAACAGCTGT GTCTACTTCT
    GTTTCTCAGC CTGGTATGGT TCATCCTTTG GTCATCTCAT CAGCTGTAGT TTCTACACCT
    GGCCTCCCCC AACCAGTAGT ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAATATC
    CCACCCTTGG TACAACCTGT TGTTAATGTG CCTGCTGTAC AGCAAACACT CATTCACAGT
    CAGCCTCAAC CAGCTCTGCT TCCCAACCAG CCTCACACAC ACTGTCCTGA AATGGATGCT
    GATACCCAAT CCAAAGCTCC TGGGATTGAT GATATAAAGA CTCTGGAGGA AAAGCTGAGA
    TCTCTCTTTA GTGAACACAG CTCATCTGGA ACTCAGCATG CTTCTGTCTC CCTAGAGACA
    CCTTTAGTAG TAGAGACGAC TGTCACACCG GGCATCACTA CCACTGCTGT AGCACCAAGT
    AAACTCATGA CTTCCACTAC AAGTACTTGC TTACCACCAA CCAGTTTACC ATTAGGAGCA
    GCTGGCATGC CAGTTATGCC AGTAGGCACA CCTGGGCAGG TTTCTACCCC AGGCACACAC
    GCTTCAGCCC CAGTCGGCAC TGCAACAGGA GTAAAACCTG GAACAACTCC CCCAAAGCCA
    ACTAAGACTG TGGTGCCACC AGTGGGTACT GAACTTTCAG CCGGTACTGT ACCCTGTGAG
    CAGCTGCCAC CTTTTCCTGG ACCTTCTCTA ATTCAGCCTT TGTCTACGAT GTCTTCAACA
    ACAGTTACAG AAGCAGGGAC TCGGCTTCAA AAGGATGGCA CAGAAGGTCA TGTCACAGCA
    ACTAGTTCAG GAGCTGGTGT TGTTAAGATG GGGCGATTTC AGGTTTCAGT TACAATGGAT
    GATGCCCAGA AAGAACGTAA AAATAGGTCA GAAGATACAA AGTCGGTTCA TTTTGAATCT
    AGCACATCAG AGTCATCAGT TCTTTCAAGT AGTAGTCCAG AGAGTACCCT GGTAAAGCCA
    GAGCCTAATG GAATTAGTAT TTCTGGCATC TCCTTAGATG TCCCAGACAG CACCCACAAA
    GCACCTACCC CAGAAGCAAA GTCAGATGCA GGACAGCCTA CCAAGGTGGG ACGTTTTCAG
    GTGACAACTA CAGCCAACAA AGTGGGTCGT TTCTCTGTTT CAAGAACTGA GGACAAGGTC
    ACAGAGCTCA AAAAAGAGGG ACCAGTGACC TCTCCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGTGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATTTG
    AATGGGCCAT CTTCAGACCT GGAGGCTGCC TTTCTAAGTA GGGGTACAGA GGATGGCTCA
    GGTAGTCCAC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCGTCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCA TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTACGA CGACTACGAG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACTAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CGGGGAGAAG AAGGAGACCC
    ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC AGCAGCTCAT TGGGCAATAA AAGCCCACAG
    CTTTCAGGCA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TACACCCCCA ACAGACCCTC
    CATCCCGCAG GAAACACCCC CGAGACTGGG CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGGACCAGC AGCACAAATA CCGTTGGAGG AACAGTGAGT
    AGCCAGGCTG CCCAGGCTCA GCCTCCTGCT ATGACTTCCA GTAGGAAGGG CACATTCACA
    GATGACCTGC ACAAGTTAGT AGACAACTGG GCCCGAGATG CCATGAATCT TTCAGGCAGG
    AGAGGCAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT
    CCTGGGCAGC TGTGTGTTCC CATGACCTCA AACCTGGGTG GCTCTACCCC CATCTCTGCA
    GCATCTGCTA CCTCTCTAGG TCACTTCACC AAATCCATGT GTCCCCCACA GCAGTATGGT
    TTTCCACCTG CCCCATTTGG CACTCAGTGG AGTGGAACAG GTGGTCCAGC ACCGCAGCCA
    CTTGGCCAGT TCCAACCTGT AGGAACAGCA TCCTTGCAAA ATTTCAACAT CAGCAATTTG
    CAGAAGTCCA TCAGCAACCC CCCAGGTTCC AATCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15955
    Clone ID Related Accession (Same CDS sequence) NM_001199084.1
    Accession Version NM_001199084.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133738.1, BC171955.1, AC113092.25, CN693598.1, BC145282.1 and BC054836.1. Transcript Variant: This variant (5) represents the longest transcript and encodes the longest isoform (5). This variant represents the exon combination of the dorsal root ganglia and sciatic nerve variant described in Figure 2F of PubMed ID 18521183. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGCCTCAGTC CATGGCGCAT
    CCGTGTGGGG GGACCCCAAC ATACCCAGAA TCACAGATAT TTTTCCCAAC TATTCATGAA
    CGTCCAGTTT CTTTTTCACC ACCTCCCACC TGTCCACCGA AAGTAGCCAT TTCCCAACGA
    CGTAAGAGCA CCTCCTTCCT GGAAGCCCAA ACTCGCCACT TCCAACCCCT GCTGAGGACT
    GTTGGCCAAA ATCATCTTCC ACCTGGTAGC AGCCCAACTA ACTGGACACC AGAGGCCATA
    GTTATGTTGG GTGCTACAGC CAATAGAGTA AATAGAGAGC TATGTGAGAT GCAGGTCCAA
    CCTGTGTTTG AGCCAACCCA GATTTACAGT GACTATAGAC CTGGACTAGT ACTGGCAGAA
    GAAGCTCACT ATTTTATTCC TCAGGAAACA GTATATCTAG CTGGGGTGCA TTACCAGGCC
    CAGGTGGCAG GACAGTATGA GGGCATTTCA TACAACTCAC CAGTACTGTC AAGTCCTATG
    AAACAGATAT CTGAACAGAA GCCAGTGCCG GGGGGCCCTG CCTCAAGTTC TGTCTTTGAA
    TTTCCTTCTG GACAGGCTTT CCTGGTAGGA CACCTTCAGA ATTTAAGATT AGATTCTGGA
    CCAAGTCCAG CATCACCCCT CTCTAGTATT TCTGCGCCTA ACAGTACAGA TGCTACACAT
    CTGAAATTTC ACCCTGTCTT TGTACCTCAT TCTGCACCAG CTGTGTTAAC TAATAGCAAT
    GAGAACAGAA GCAACTGTGT ATTTGAATTT CATGCTCAAA CACCAAGTTC TTCAGGAGAA
    GGAGGTGGGA TTTTACCTCA ACGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTCAAAT
    CAGGAAGAAC TGTCTCCTCA GTCAGTTGGA TTACATTGCC ACCTGCAGCC TGTTACTGAA
    GAACAGCGTA ATAACCATGC CCCCGAATTG ACCATTTCTG TGGTAGAACC TATGGGACAA
    ATCTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CTCAAATTAC TTCTTCAGAT
    CTCAGTGATT TTCAATCACC TCCCCCTACA GGGGGGACAG CTGCACCTTT TGGCTCTGAC
    GTCTCATTGC CCTTTATTCG CCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTATTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTATCTG CTTCATATTC TTCAGGAGGA
    TCTACACTCC ATCCACAGGC ACAGGGGCAG AACCAGGGCC AGCCATCAAG TAGTTTGGCT
    GGGGTTCTAT CTTCACAACC TATCCAACAT CCTCAGCAGC AGGGAATACA GCCAACTGTT
    CCTTCTCAAC AGGCAGTACA GTATTCACTT CCACAAGCAG CATCTTCCAG TGAAGGTACT
    ACTGCACAGC CAGTGAGTCA GCCTCAAGTG TCAGCTGGAA CACAGGGCTT CCCATCTCGA
    CTGCCACCAC AATACCCAGG AGATTCAAAT ATTGCTCCCT CTTCCAACGT GGCTTCTGTT
    TGCATCCATT CTACAGTCCT AGCCCCTCCT TCTATGCCGA CAGAAGCATT GGCCACCCAA
    GGTTACTTTC CTACAGTAGT GCAGCCTTAT GTGGAATCAA CTCCTTTGGT TCCTATGGGC
    AGTGTAGGAG GCCAGGTTCA AGTGTCCCAA CCAGCAGTGA GTTTAACGCA ACAACCCCCA
    ACTACATCCT CCCAGCAAGC AGTTCTGGAG AGTACTCAGG GAGTCTCTCA GGCTGCTCCT
    CCAGAACAAA CTCCAATAAC ACAGTCACAA CCTACTCAGC CTGTTCCATT GGTTACCTCT
    GCAGACAGTG CACATTCAGA CGTTGCTTCT GGTATGAGTG ATGGCAATGA GAATGCTCCA
    TCATCCAGCG GGAGGCATGA AGGGAGGACA ACAAAACGGC ATTATAGAAA ATCCGTAAGA
    AGTCGCTCTC GGCATGAAAA GACTTCTCGC CCAAAACTGA GGATTTTAAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATCGGAAGAT GGTTACATTT
    AAATTTGACC TAGATGGTGA CAACCCTGAA GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCTTTT GTGGCTCAAG TGCGGGAAAT TATTGAAAAG
    GCTGATGAAA TGTTAAGTGA GGATGTCAGT GTGGAGCCTG AGGGTGACCA GGGATTGGAG
    AGTCTGCAAG GAAAGGATGA CTATGGCTTC CCAGGTTCTC AGAAATTAGA AGGAGAGTTC
    AAGCAACCAA TTGCTGTATC TTCCATGCCA CAACAAATAG GTGTTCCTAC CAGTTCTTTA
    ACGCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTGA GTCCTGTACC TGAAAGTAGG
    TTACGAGAAT CAAAAGTTTT CACCAGTGAC ATATCAGATC CAGTTGTGGC CTCTACATCT
    CAGGCCCCTG GAATGAACTT ATCTCACTCT GCTTCATCCC TCAGTCTCCA GCAGGCTTTT
    TCTGAACTGA AACATGGTCA GATGACTGAA GGACCCAATA CAGCACCTCC AAATTTTAAT
    CACATGGCGG GACCAACATT TTCTCCCTTT TTGGCTAGCA TTGCTGGTGT CCAAACTGTA
    GCAGCATCCA CACCGTCAGT ATCAGTCCCT ATAACAAGCA GTCCTCTTAA TGACATTTCC
    ACATCAGTGA TGCAGTCTGA GACTGCACTG CCCACTGAAA AAGGCATCGT GGGAGTTACC
    ACTACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CAATGTCTGT GTCTGAGTCA
    CCAACATCTT CCAGTGCGGT TTCAAGTTCT ACAGTACCTG CAGTTGTCAC TGTGTCCACA
    CCATCCCAGC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCAAGTAC AGGCAGTTTT
    CCTCCTGGAA CATTTTCTAC AACTACAGCT ACTACAATGG GTAGTGTGGT GGCCCCAGAT
    GCTAAGCCTC CAACTGTATT ATTGCAACAG GTAGCAAGCA ATACTGCTGG GGTTGCCATA
    GTGACATCAG TCTCTACCAC CACTCCATTC CCAGGCATGG CTTCACAGCC ATCCCTTCCA
    CTTAGTAGCA GCACCTCTGC TCCTACTCTA GCTGAAACAA TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG TGCGCCATCC
    TCTTCCTCCT CCTCTGGAAC AGCTGTGTCT ACTTCTGTTT CTCAGCCTGG TATGGTTCAT
    CCTTTGGTCA TCTCATCAGC TGTAGTTTCT ACACCTGGCC TCCCCCAACC AGTAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGTT
    AATGTGCCTG CTGTACAGCA AACACTCATT CACAGTCAGC CTCAACCAGC TCTGCTTCCC
    AACCAGCCTC ACACACACTG TCCTGAAATG GATGCTGATA CCCAATCCAA AGCTCCTGGG
    ATTGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCTC TCTTTAGTGA ACACAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTA GAGACACCTT TAGTAGTAGA GACGACTGTC
    ACACCGGGCA TCACTACCAC TGCTGTAGCA CCAAGTAAAC TCATGACTTC CACTACAAGT
    ACTTGCTTAC CACCAACCAG TTTACCATTA GGAGCAGCTG GCATGCCAGT TATGCCAGTA
    GGCACACCTG GGCAGGTTTC TACCCCAGGC ACACACGCTT CAGCCCCAGT CGGCACTGCA
    ACAGGAGTAA AACCTGGAAC AACTCCCCCA AAGCCAACTA AGACTGTGGT GCCACCAGTG
    GGTACTGAAC TTTCAGCCGG TACTGTACCC TGTGAGCAGC TGCCACCTTT TCCTGGACCT
    TCTCTAATTC AGTCCCAGCA ACCACTAGAA GATCTTGATG CTCAGTTGAG AAGAACACTT
    AGTCCAGAGA CTATTACAGT GGCACCTGCA GTTGGTCCTT TGTCTACGAT GTCTTCAACA
    ACAGTTACAG AAGCAGGGAC TCGGCTTCAA AAGGATGGCA CAGAAGGTCA TGTCACAGCA
    ACTAGTTCAG GAGCTGGTGT TGTTAAGATG GGGCGATTTC AGGTTTCAGT TACAATGGAT
    GATGCCCAGA AAGAACGTAA AAATAGGTCA GAAGATACAA AGTCGGTTCA TTTTGAATCT
    AGCACATCAG AGTCATCAGT TCTTTCAAGT AGTAGTCCAG AGAGTACCCT GGTAAAGCCA
    GAGCCTAATG GAATTAGTAT TTCTGGCATC TCCTTAGATG TCCCAGACAG CACCCACAAA
    GCACCTACCC CAGAAGCAAA GTCAGATGCA GGACAGCCTA CCAAGGTGGG ACGTTTTCAG
    GTGACAACTA CAGCCAACAA AGTGGGTCGT TTCTCTGTTT CAAGAACTGA GGACAAGGTC
    ACAGAGCTCA AAAAAGAGGG ACCAGTGACC TCTCCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGTGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATTTG
    AATGGGCCAT CTTCAGACCT GGAGGCTGCC TTTCTAAGTA GGGGTACAGA GGATGGCTCA
    GGTAGTCCAC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCGTCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCA TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTACGA CGACTACGAG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACTAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CGGGGAGAAG AAGGAGACCC
    ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC AGCAGCTCAT TGGGCAATAA AAGCCCACAG
    CTTTCAGGCA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TACACCCCCA ACAGACCCTC
    CATCCCGCAG GAAACACCCC CGAGACTGGG CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGGACCAGC AGCACAAATA CCGTTGGAGG AACAGTGAGT
    AGCCAGGCTG CCCAGGCTCA GCCTCCTGCT ATGACTTCCA GTAGGAAGGG CACATTCACA
    GATGACCTGC ACAAGTTAGT AGACAACTGG GCCCGAGATG CCATGAATCT TTCAGGCAGG
    AGAGGCAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT
    CCTGGGCAGC TGTGTGTTCC CATGACCTCA AACCTGGGTG GCTCTACCCC CATCTCTGCA
    GCATCTGCTA CCTCTCTAGG TCACTTCACC AAATCCATGT GTCCCCCACA GCAGTATGGT
    TTTCCACCTG CCCCATTTGG CACTCAGTGG AGTGGAACAG GTGGTCCAGC ACCGCAGCCA
    CTTGGCCAGT TCCAACCTGT AGGAACAGCA TCCTTGCAAA ATTTCAACAT CAGCAATTTG
    CAGAAGTCCA TCAGCAACCC CCCAGGTTCC AATCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186013.1
    CDS1024..8931
    Translation

    Target ORF information:

    RefSeq Version NM_001199084.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199084.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGCCTCAGTC CATGGCGCAT
    CCGTGTGGGG GGACCCCAAC ATACCCAGAA TCACAGATAT TTTTCCCAAC TATTCATGAA
    CGTCCAGTTT CTTTTTCACC ACCTCCCACC TGTCCACCGA AAGTAGCCAT TTCCCAACGA
    CGTAAGAGCA CCTCCTTCCT GGAAGCCCAA ACTCGCCACT TCCAACCCCT GCTGAGGACT
    GTTGGCCAAA ATCATCTTCC ACCTGGTAGC AGCCCAACTA ACTGGACACC AGAGGCCATA
    GTTATGTTGG GTGCTACAGC CAATAGAGTA AATAGAGAGC TATGTGAGAT GCAGGTCCAA
    CCTGTGTTTG AGCCAACCCA GATTTACAGT GACTATAGAC CTGGACTAGT ACTGGCAGAA
    GAAGCTCACT ATTTTATTCC TCAGGAAACA GTATATCTAG CTGGGGTGCA TTACCAGGCC
    CAGGTGGCAG GACAGTATGA GGGCATTTCA TACAACTCAC CAGTACTGTC AAGTCCTATG
    AAACAGATAT CTGAACAGAA GCCAGTGCCG GGGGGCCCTG CCTCAAGTTC TGTCTTTGAA
    TTTCCTTCTG GACAGGCTTT CCTGGTAGGA CACCTTCAGA ATTTAAGATT AGATTCTGGA
    CCAAGTCCAG CATCACCCCT CTCTAGTATT TCTGCGCCTA ACAGTACAGA TGCTACACAT
    CTGAAATTTC ACCCTGTCTT TGTACCTCAT TCTGCACCAG CTGTGTTAAC TAATAGCAAT
    GAGAACAGAA GCAACTGTGT ATTTGAATTT CATGCTCAAA CACCAAGTTC TTCAGGAGAA
    GGAGGTGGGA TTTTACCTCA ACGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTCAAAT
    CAGGAAGAAC TGTCTCCTCA GTCAGTTGGA TTACATTGCC ACCTGCAGCC TGTTACTGAA
    GAACAGCGTA ATAACCATGC CCCCGAATTG ACCATTTCTG TGGTAGAACC TATGGGACAA
    ATCTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CTCAAATTAC TTCTTCAGAT
    CTCAGTGATT TTCAATCACC TCCCCCTACA GGGGGGACAG CTGCACCTTT TGGCTCTGAC
    GTCTCATTGC CCTTTATTCG CCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTATTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTATCTG CTTCATATTC TTCAGGAGGA
    TCTACACTCC ATCCACAGGC ACAGGGGCAG AACCAGGGCC AGCCATCAAG TAGTTTGGCT
    GGGGTTCTAT CTTCACAACC TATCCAACAT CCTCAGCAGC AGGGAATACA GCCAACTGTT
    CCTTCTCAAC AGGCAGTACA GTATTCACTT CCACAAGCAG CATCTTCCAG TGAAGGTACT
    ACTGCACAGC CAGTGAGTCA GCCTCAAGTG TCAGCTGGAA CACAGGGCTT CCCATCTCGA
    CTGCCACCAC AATACCCAGG AGATTCAAAT ATTGCTCCCT CTTCCAACGT GGCTTCTGTT
    TGCATCCATT CTACAGTCCT AGCCCCTCCT TCTATGCCGA CAGAAGCATT GGCCACCCAA
    GGTTACTTTC CTACAGTAGT GCAGCCTTAT GTGGAATCAA CTCCTTTGGT TCCTATGGGC
    AGTGTAGGAG GCCAGGTTCA AGTGTCCCAA CCAGCAGTGA GTTTAACGCA ACAACCCCCA
    ACTACATCCT CCCAGCAAGC AGTTCTGGAG AGTACTCAGG GAGTCTCTCA GGCTGCTCCT
    CCAGAACAAA CTCCAATAAC ACAGTCACAA CCTACTCAGC CTGTTCCATT GGTTACCTCT
    GCAGACAGTG CACATTCAGA CGTTGCTTCT GGTATGAGTG ATGGCAATGA GAATGCTCCA
    TCATCCAGCG GGAGGCATGA AGGGAGGACA ACAAAACGGC ATTATAGAAA ATCCGTAAGA
    AGTCGCTCTC GGCATGAAAA GACTTCTCGC CCAAAACTGA GGATTTTAAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATCGGAAGAT GGTTACATTT
    AAATTTGACC TAGATGGTGA CAACCCTGAA GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCTTTT GTGGCTCAAG TGCGGGAAAT TATTGAAAAG
    GCTGATGAAA TGTTAAGTGA GGATGTCAGT GTGGAGCCTG AGGGTGACCA GGGATTGGAG
    AGTCTGCAAG GAAAGGATGA CTATGGCTTC CCAGGTTCTC AGAAATTAGA AGGAGAGTTC
    AAGCAACCAA TTGCTGTATC TTCCATGCCA CAACAAATAG GTGTTCCTAC CAGTTCTTTA
    ACGCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTGA GTCCTGTACC TGAAAGTAGG
    TTACGAGAAT CAAAAGTTTT CACCAGTGAC ATATCAGATC CAGTTGTGGC CTCTACATCT
    CAGGCCCCTG GAATGAACTT ATCTCACTCT GCTTCATCCC TCAGTCTCCA GCAGGCTTTT
    TCTGAACTGA AACATGGTCA GATGACTGAA GGACCCAATA CAGCACCTCC AAATTTTAAT
    CACATGGCGG GACCAACATT TTCTCCCTTT TTGGCTAGCA TTGCTGGTGT CCAAACTGTA
    GCAGCATCCA CACCGTCAGT ATCAGTCCCT ATAACAAGCA GTCCTCTTAA TGACATTTCC
    ACATCAGTGA TGCAGTCTGA GACTGCACTG CCCACTGAAA AAGGCATCGT GGGAGTTACC
    ACTACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CAATGTCTGT GTCTGAGTCA
    CCAACATCTT CCAGTGCGGT TTCAAGTTCT ACAGTACCTG CAGTTGTCAC TGTGTCCACA
    CCATCCCAGC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCAAGTAC AGGCAGTTTT
    CCTCCTGGAA CATTTTCTAC AACTACAGCT ACTACAATGG GTAGTGTGGT GGCCCCAGAT
    GCTAAGCCTC CAACTGTATT ATTGCAACAG GTAGCAAGCA ATACTGCTGG GGTTGCCATA
    GTGACATCAG TCTCTACCAC CACTCCATTC CCAGGCATGG CTTCACAGCC ATCCCTTCCA
    CTTAGTAGCA GCACCTCTGC TCCTACTCTA GCTGAAACAA TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG TGCGCCATCC
    TCTTCCTCCT CCTCTGGAAC AGCTGTGTCT ACTTCTGTTT CTCAGCCTGG TATGGTTCAT
    CCTTTGGTCA TCTCATCAGC TGTAGTTTCT ACACCTGGCC TCCCCCAACC AGTAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGTT
    AATGTGCCTG CTGTACAGCA AACACTCATT CACAGTCAGC CTCAACCAGC TCTGCTTCCC
    AACCAGCCTC ACACACACTG TCCTGAAATG GATGCTGATA CCCAATCCAA AGCTCCTGGG
    ATTGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCTC TCTTTAGTGA ACACAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTA GAGACACCTT TAGTAGTAGA GACGACTGTC
    ACACCGGGCA TCACTACCAC TGCTGTAGCA CCAAGTAAAC TCATGACTTC CACTACAAGT
    ACTTGCTTAC CACCAACCAG TTTACCATTA GGAGCAGCTG GCATGCCAGT TATGCCAGTA
    GGCACACCTG GGCAGGTTTC TACCCCAGGC ACACACGCTT CAGCCCCAGT CGGCACTGCA
    ACAGGAGTAA AACCTGGAAC AACTCCCCCA AAGCCAACTA AGACTGTGGT GCCACCAGTG
    GGTACTGAAC TTTCAGCCGG TACTGTACCC TGTGAGCAGC TGCCACCTTT TCCTGGACCT
    TCTCTAATTC AGTCCCAGCA ACCACTAGAA GATCTTGATG CTCAGTTGAG AAGAACACTT
    AGTCCAGAGA CTATTACAGT GGCACCTGCA GTTGGTCCTT TGTCTACGAT GTCTTCAACA
    ACAGTTACAG AAGCAGGGAC TCGGCTTCAA AAGGATGGCA CAGAAGGTCA TGTCACAGCA
    ACTAGTTCAG GAGCTGGTGT TGTTAAGATG GGGCGATTTC AGGTTTCAGT TACAATGGAT
    GATGCCCAGA AAGAACGTAA AAATAGGTCA GAAGATACAA AGTCGGTTCA TTTTGAATCT
    AGCACATCAG AGTCATCAGT TCTTTCAAGT AGTAGTCCAG AGAGTACCCT GGTAAAGCCA
    GAGCCTAATG GAATTAGTAT TTCTGGCATC TCCTTAGATG TCCCAGACAG CACCCACAAA
    GCACCTACCC CAGAAGCAAA GTCAGATGCA GGACAGCCTA CCAAGGTGGG ACGTTTTCAG
    GTGACAACTA CAGCCAACAA AGTGGGTCGT TTCTCTGTTT CAAGAACTGA GGACAAGGTC
    ACAGAGCTCA AAAAAGAGGG ACCAGTGACC TCTCCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGTGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATTTG
    AATGGGCCAT CTTCAGACCT GGAGGCTGCC TTTCTAAGTA GGGGTACAGA GGATGGCTCA
    GGTAGTCCAC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCGTCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCA TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTACGA CGACTACGAG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACTAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CGGGGAGAAG AAGGAGACCC
    ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC AGCAGCTCAT TGGGCAATAA AAGCCCACAG
    CTTTCAGGCA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TACACCCCCA ACAGACCCTC
    CATCCCGCAG GAAACACCCC CGAGACTGGG CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGGACCAGC AGCACAAATA CCGTTGGAGG AACAGTGAGT
    AGCCAGGCTG CCCAGGCTCA GCCTCCTGCT ATGACTTCCA GTAGGAAGGG CACATTCACA
    GATGACCTGC ACAAGTTAGT AGACAACTGG GCCCGAGATG CCATGAATCT TTCAGGCAGG
    AGAGGCAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT
    CCTGGGCAGC TGTGTGTTCC CATGACCTCA AACCTGGGTG GCTCTACCCC CATCTCTGCA
    GCATCTGCTA CCTCTCTAGG TCACTTCACC AAATCCATGT GTCCCCCACA GCAGTATGGT
    TTTCCACCTG CCCCATTTGG CACTCAGTGG AGTGGAACAG GTGGTCCAGC ACCGCAGCCA
    CTTGGCCAGT TCCAACCTGT AGGAACAGCA TCCTTGCAAA ATTTCAACAT CAGCAATTTG
    CAGAAGTCCA TCAGCAACCC CCCAGGTTCC AATCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16018
    Clone ID Related Accession (Same CDS sequence) NM_001199083.1
    Accession Version NM_001199083.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133738.1, BC171955.1, AC113092.25, CN693598.1 and BC054836.1. Transcript Variant: This variant (4) has multiple differences in the coding region but maintains the reading frame compared to variant 5. This variant represents the exon combination of the brain and spinal cord variant described in Figure 2F of PubMed ID 18521183. This variant encodes isoform 4, which is shorter than isoform 5 but has the same N- and C-termini. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGCCTCGTCG TGGCCGTAGC
    ATGTCGGTTT GTGTTCCCCA TCTTTCTGCT GTTCCCTCTC TATCCCGCAT CTCTCCCAGT
    GCTCCTTCCA CCCCACCACC AGTGCTGTCT GCACCTCTTT GTCCTTCCCT CCTTCGGACT
    GCCCCTGAGG AAACTTTTGC CGAAAAACTC TCTAAAGCAT TGGAGAGTGT CCTGCCTATG
    CACTCTGCCT CCCAGCGCAA GCACCGACGC TCCAGCCTGC CTTCCCTCTT TGTCACTACT
    CCTCAGTCCA TGGCGCATCC GTGTGGGGGG ACCCCAACAT ACCCAGAATC ACAGATATTT
    TTCCCAACTA TTCATGAACG TCCAGTTTCT TTTTCACCAC CTCCCACCTG TCCACCGAAA
    GTAGCCATTT CCCAACGACG TAAGAGCACC TCCTTCCTGG AAGCCCAAAC TCGCCACTTC
    CAACCCCTGC TGAGGACTGT TGGCCAAAAT CATCTTCCAC CTGGTAGCAG CCCAACTAAC
    TGGACACCAG AGGCCATAGT TATGTTGGGT GCTACAGCCA ATAGAGTAAA TAGAGAGCTA
    TGTGAGATGC AGGTCCAACC TGTGTTTGAG CCAACCCAGA TTTACAGTGA CTATAGACCT
    GGACTAGTAC TGGCAGAAGA AGCTCACTAT TTTATTCCTC AGGAAACAGT ATATCTAGCT
    GGGGTGCATT ACCAGGCCCA GGTGGCAGGA CAGTATGAGG GCATTTCATA CAACTCACCA
    GTACTGTCAA GTCCTATGAA ACAGATATCT GAACAGAAGC CAGTGCCGGG GGGCCCTGCC
    TCAAGTTCTG TCTTTGAATT TCCTTCTGGA CAGGCTTTCC TGGTAGGACA CCTTCAGAAT
    TTAAGATTAG ATTCTGGACC AAGTCCAGCA TCACCCCTCT CTAGTATTTC TGCGCCTAAC
    AGTACAGATG CTACACATCT GAAATTTCAC CCTGTCTTTG TACCTCATTC TGCACCAGCT
    GTGTTAACTA ATAGCAATGA GAACAGAAGC AACTGTGTAT TTGAATTTCA TGCTCAAACA
    CCAAGTTCTT CAGGAGAAGG AGGTGGGATT TTACCTCAAC GTGTTTACCG AAATCGGCAG
    GTTGCAGTGG ACTCAAATCA GGAAGAACTG TCTCCTCAGT CAGTTGGATT ACATTGCCAC
    CTGCAGCCTG TTACTGAAGA ACAGCGTAAT AACCATGCCC CCGAATTGAC CATTTCTGTG
    GTAGAACCTA TGGGACAAAT CTGGCCAATA GGAAGCCCAG AATATTCCAG TGATTCCTCT
    CAAATTACTT CTTCAGATCT CAGTGATTTT CAATCACCTC CCCCTACAGG GGGGACAGCT
    GCACCTTTTG GCTCTGACGT CTCATTGCCC TTTATTCGCC TGCCTCAGAC AGTGTTACAA
    GAATCCCCAC TATTCTTCTG TTTCCCCCAA GGAACCACAT CTCAGCAGGT CTTATCTGCT
    TCATATTCTT CAGGAGGATC TACACTCCAT CCACAGGCAC AGGGGCAGAA CCAGGGCCAG
    CCATCAAGTA GTTTGGCTGG GGTTCTATCT TCACAACCTA TCCAACATCC TCAGCAGCAG
    GGAATACAGC CAACTGTTCC TTCTCAACAG GCAGTACAGT ATTCACTTCC ACAAGCAGCA
    TCTTCCAGTG AAGGTACTAC TGCACAGCCA GTGAGTCAGC CTCAAGTGTC AGCTGGAACA
    CAGAGTACTC AGGGAGTCTC TCAGGCTGCT CCTCCAGAAC AAACTCCAAT AACACAGTCA
    CAACCTACTC AGCCTGTTCC ATTGGTTACC TCTGCAGACA GTGCACATTC AGACGTTGCT
    TCTGGTATGA GTGATGGCAA TGAGAATGCT CCATCATCCA GCGGGAGGCA TGAAGGGAGG
    ACAACAAAAC GGCATTATAG AAAATCCGTA AGAAGTCGCT CTCGGCATGA AAAGACTTCT
    CGCCCAAAAC TGAGGATTTT AAATGTTTCA AATAAAGGAG ACCGAGTAGT AGAATGTCAA
    TTAGAGACTC ATAATCGGAA GATGGTTACA TTTAAATTTG ACCTAGATGG TGACAACCCT
    GAAGAGATAG CAACAATTAT GGTGAACAAT GACTTTATTC TAGCAATAGA GAGAGAGTCT
    TTTGTGGCTC AAGTGCGGGA AATTATTGAA AAGGCTGATG AAATGTTAAG TGAGGATGTC
    AGTGTGGAGC CTGAGGGTGA CCAGGGATTG GAGAGTCTGC AAGGAAAGGA TGACTATGGC
    TTCCCAGGTT CTCAGAAATT AGAAGGAGAG TTCAAGCAAC CAATTGCTGT ATCTTCCATG
    CCACAACAAA TAGGTGTTCC TACCAGTTCT TTAACGCAAG TTGTTCATTC TGCTGGAAGA
    CGGTTTATAG TGAGTCCTGT ACCTGAAAGT AGGTTACGAG AATCAAAAGT TTTCACCAGT
    GACATATCAG ATCCAGTTGT GGCCTCTACA TCTCAGGCCC CTGGAATGAA CTTATCTCAC
    TCTGCTTCAT CCCTCAGTCT CCAGCAGGCT TTTTCTGAAC TGAAACATGG TCAGATGACT
    GAAGGACCCA ATACAGCACC TCCAAATTTT AATCACATGG CGGGACCAAC ATTTTCTCCC
    TTTTTGGCTA GCATTGCTGG TGTCCAAACT GTAGCAGCAT CCACACCGTC AGTATCAGTC
    CCTATAACAA GCAGTCCTCT TAATGACATT TCCACATCAG TGATGCAGTC TGAGACTGCA
    CTGCCCACTG AAAAAGGCAT CGTGGGAGTT ACCACTACCA GCACAGGTGT GGTAGCTTCA
    GGTGGTCTCA CCACAATGTC TGTGTCTGAG TCACCAACAT CTTCCAGTGC GGTTTCAAGT
    TCTACAGTAC CTGCAGTTGT CACTGTGTCC ACACCATCCC AGCCAGTTCA GGCTTCCACA
    TCTGGGAGCA TTGCTTCAAG TACAGGCAGT TTTCCTCCTG GAACATTTTC TACAACTACA
    GCTACTACAA TGGGTAGTGT GGTGGCCCCA GATGCTAAGC CTCCAACTGT ATTATTGCAA
    CAGGTAGCAA GCAATACTGC TGGGGTTGCC ATAGTGACAT CAGTCTCTAC CACCACTCCA
    TTCCCAGGCA TGGCTTCACA GCCATCCCTT CCACTTAGTA GCAGCACCTC TGCTCCTACT
    CTAGCTGAAA CAATGGTGGT TAGTGCACAC TCACTAGACA AGGCATCTCA CAGTAGTACA
    GCAGGATTGG GTTTGTCCTT TTGTGCGCCA TCCTCTTCCT CCTCCTCTGG AACAGCTGTG
    TCTACTTCTG TTTCTCAGCC TGGTATGGTT CATCCTTTGG TCATCTCATC AGCTGTAGTT
    TCTACACCTG GCCTCCCCCA ACCAGTAGTA CCTACTTCTA CACCTTTATT ACCCCAAGTA
    CCTAATATCC CACCCTTGGT ACAACCTGTT GTTAATGTGC CTGCTGTACA GCAAACACTC
    ATTCACAGTC AGCCTCAACC AGCTCTGCTT CCCAACCAGC CTCACACACA CTGTCCTGAA
    ATGGATGCTG ATACCCAATC CAAAGCTCCT GGGATTGATG ATATAAAGAC TCTGGAGGAA
    AAGCTGAGAT CTCTCTTTAG TGAACACAGC TCATCTGGAA CTCAGCATGC TTCTGTCTCC
    CTAGAGACAC CTTTAGTAGT AGAGACGACT GTCACACCGG GCATCACTAC CACTGCTGTA
    GCACCAAGTA AACTCATGAC TTCCACTACA AGTACTTGCT TACCACCAAC CAGTTTACCA
    TTAGGAGCAG CTGGCATGCC AGTTATGCCA GTAGGCACAC CTGGGCAGGT TTCTACCCCA
    GGCACACACG CTTCAGCCCC AGTCGGCACT GCAACAGGAG TAAAACCTGG AACAACTCCC
    CCAAAGCCAA CTAAGACTGT GGTGCCACCA GTGGGTACTG AACTTTCAGC CGGTACTGTA
    CCCTGTGAGC AGCTGCCACC TTTTCCTGGA CCTTCTCTAA TTCAGTCCCA GCAACCACTA
    GAAGATCTTG ATGCTCAGTT GAGAAGAACA CTTAGTCCAG AGACTATTAC AGTGGCACCT
    GCAGTTGGTC CTTTGTCTAC GATGTCTTCA ACAACAGTTA CAGAAGCAGG GACTCGGCTT
    CAAAAGGATG GCACAGAAGG TCATGTCACA GCAACTAGTT CAGGAGCTGG TGTTGTTAAG
    ATGGGGCGAT TTCAGGTTTC AGTTACAATG GATGATGCCC AGAAAGAACG TAAAAATAGG
    TCAGAAGATA CAAAGTCGGT TCATTTTGAA TCTAGCACAT CAGAGTCATC AGTTCTTTCA
    AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG CCAGAGCCTA ATGGAATTAG TATTTCTGGC
    ATCTCCTTAG ATGTCCCAGA CAGCACCCAC AAAGCACCTA CCCCAGAAGC AAAGTCAGAT
    GCAGGACAGC CTACCAAGGT GGGACGTTTT CAGGTGACAA CTACAGCCAA CAAAGTGGGT
    CGTTTCTCTG TTTCAAGAAC TGAGGACAAG GTCACAGAGC TCAAAAAAGA GGGACCAGTG
    ACCTCTCCTC CTTTCAGAGA TTCAGAACAA ACTGTTATTC CTGCTGTGAT ACCAAAGAAG
    GAGAAGCCTG AGCTGGCAGA ACCTTCCCAT TTGAATGGGC CATCTTCAGA CCTGGAGGCT
    GCCTTTCTAA GTAGGGGTAC AGAGGATGGC TCAGGTAGTC CACACTCTCC CCCTCACCTG
    TGTTCAAAGA GCCTTCCCGT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC
    TCATACATGA GTAGTGACAA TGAGTCCGAC ATTGAAGATG AAGACTTAAG GTTAGAACTA
    CGACGACTAC GAGAAAAACA TCTTAAAGAG ATTCAGGACC TGCAGAGTCG CCAGAAGCAT
    GAAATTGAAT CTTTGTATAC TAAACTGGGC AAGGTTCCCC CTGCTGTCAT TATTCCCCCA
    GCTGCTCCTC TGTCGGGGAG AAGAAGGAGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT
    CGCAGCAGCT CATTGGGCAA TAAAAGCCCA CAGCTTTCAG GCAACCTGTC TGGTCAGAGT
    GGAACTTCAG TCTTACACCC CCAACAGACC CTCCATCCCG CAGGAAACAC CCCCGAGACT
    GGGCACAACC AGCTGTTACA GCCTCTTAAG CCATCTCCCT CCAGTGACAA CCTCTATTCA
    GCCTTTACCA GTGATGGTGC CATTTCAGTA CCAAGCCTTT CTGCTCCAGG TCAAGGGACC
    AGCAGCACAA ATACCGTTGG AGGAACAGTG AGTAGCCAGG CTGCCCAGGC TCAGCCTCCT
    GCTATGACTT CCAGTAGGAA GGGCACATTC ACAGATGACC TGCACAAGTT AGTAGACAAC
    TGGGCCCGAG ATGCCATGAA TCTTTCAGGC AGGAGAGGCA GCAAAGGACA CATGAATTAT
    GAGGGCCCTG GAATGGCAAG GAAGTTCTCT GCTCCTGGGC AGCTGTGTGT TCCCATGACC
    TCAAACCTGG GTGGCTCTAC CCCCATCTCT GCAGCATCTG CTACCTCTCT AGGTCACTTC
    ACCAAATCCA TGTGTCCCCC ACAGCAGTAT GGTTTTCCAC CTGCCCCATT TGGCACTCAG
    TGGAGTGGAA CAGGTGGTCC AGCACCGCAG CCACTTGGCC AGTTCCAACC TGTAGGAACA
    GCATCCTTGC AAAATTTCAA CATCAGCAAT TTGCAGAAGT CCATCAGCAA CCCCCCAGGT
    TCCAATCTAC GGACCACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186012.1
    CDS1024..8904
    Translation

    Target ORF information:

    RefSeq Version NM_001199083.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199083.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGCCTCGTCG TGGCCGTAGC
    ATGTCGGTTT GTGTTCCCCA TCTTTCTGCT GTTCCCTCTC TATCCCGCAT CTCTCCCAGT
    GCTCCTTCCA CCCCACCACC AGTGCTGTCT GCACCTCTTT GTCCTTCCCT CCTTCGGACT
    GCCCCTGAGG AAACTTTTGC CGAAAAACTC TCTAAAGCAT TGGAGAGTGT CCTGCCTATG
    CACTCTGCCT CCCAGCGCAA GCACCGACGC TCCAGCCTGC CTTCCCTCTT TGTCACTACT
    CCTCAGTCCA TGGCGCATCC GTGTGGGGGG ACCCCAACAT ACCCAGAATC ACAGATATTT
    TTCCCAACTA TTCATGAACG TCCAGTTTCT TTTTCACCAC CTCCCACCTG TCCACCGAAA
    GTAGCCATTT CCCAACGACG TAAGAGCACC TCCTTCCTGG AAGCCCAAAC TCGCCACTTC
    CAACCCCTGC TGAGGACTGT TGGCCAAAAT CATCTTCCAC CTGGTAGCAG CCCAACTAAC
    TGGACACCAG AGGCCATAGT TATGTTGGGT GCTACAGCCA ATAGAGTAAA TAGAGAGCTA
    TGTGAGATGC AGGTCCAACC TGTGTTTGAG CCAACCCAGA TTTACAGTGA CTATAGACCT
    GGACTAGTAC TGGCAGAAGA AGCTCACTAT TTTATTCCTC AGGAAACAGT ATATCTAGCT
    GGGGTGCATT ACCAGGCCCA GGTGGCAGGA CAGTATGAGG GCATTTCATA CAACTCACCA
    GTACTGTCAA GTCCTATGAA ACAGATATCT GAACAGAAGC CAGTGCCGGG GGGCCCTGCC
    TCAAGTTCTG TCTTTGAATT TCCTTCTGGA CAGGCTTTCC TGGTAGGACA CCTTCAGAAT
    TTAAGATTAG ATTCTGGACC AAGTCCAGCA TCACCCCTCT CTAGTATTTC TGCGCCTAAC
    AGTACAGATG CTACACATCT GAAATTTCAC CCTGTCTTTG TACCTCATTC TGCACCAGCT
    GTGTTAACTA ATAGCAATGA GAACAGAAGC AACTGTGTAT TTGAATTTCA TGCTCAAACA
    CCAAGTTCTT CAGGAGAAGG AGGTGGGATT TTACCTCAAC GTGTTTACCG AAATCGGCAG
    GTTGCAGTGG ACTCAAATCA GGAAGAACTG TCTCCTCAGT CAGTTGGATT ACATTGCCAC
    CTGCAGCCTG TTACTGAAGA ACAGCGTAAT AACCATGCCC CCGAATTGAC CATTTCTGTG
    GTAGAACCTA TGGGACAAAT CTGGCCAATA GGAAGCCCAG AATATTCCAG TGATTCCTCT
    CAAATTACTT CTTCAGATCT CAGTGATTTT CAATCACCTC CCCCTACAGG GGGGACAGCT
    GCACCTTTTG GCTCTGACGT CTCATTGCCC TTTATTCGCC TGCCTCAGAC AGTGTTACAA
    GAATCCCCAC TATTCTTCTG TTTCCCCCAA GGAACCACAT CTCAGCAGGT CTTATCTGCT
    TCATATTCTT CAGGAGGATC TACACTCCAT CCACAGGCAC AGGGGCAGAA CCAGGGCCAG
    CCATCAAGTA GTTTGGCTGG GGTTCTATCT TCACAACCTA TCCAACATCC TCAGCAGCAG
    GGAATACAGC CAACTGTTCC TTCTCAACAG GCAGTACAGT ATTCACTTCC ACAAGCAGCA
    TCTTCCAGTG AAGGTACTAC TGCACAGCCA GTGAGTCAGC CTCAAGTGTC AGCTGGAACA
    CAGAGTACTC AGGGAGTCTC TCAGGCTGCT CCTCCAGAAC AAACTCCAAT AACACAGTCA
    CAACCTACTC AGCCTGTTCC ATTGGTTACC TCTGCAGACA GTGCACATTC AGACGTTGCT
    TCTGGTATGA GTGATGGCAA TGAGAATGCT CCATCATCCA GCGGGAGGCA TGAAGGGAGG
    ACAACAAAAC GGCATTATAG AAAATCCGTA AGAAGTCGCT CTCGGCATGA AAAGACTTCT
    CGCCCAAAAC TGAGGATTTT AAATGTTTCA AATAAAGGAG ACCGAGTAGT AGAATGTCAA
    TTAGAGACTC ATAATCGGAA GATGGTTACA TTTAAATTTG ACCTAGATGG TGACAACCCT
    GAAGAGATAG CAACAATTAT GGTGAACAAT GACTTTATTC TAGCAATAGA GAGAGAGTCT
    TTTGTGGCTC AAGTGCGGGA AATTATTGAA AAGGCTGATG AAATGTTAAG TGAGGATGTC
    AGTGTGGAGC CTGAGGGTGA CCAGGGATTG GAGAGTCTGC AAGGAAAGGA TGACTATGGC
    TTCCCAGGTT CTCAGAAATT AGAAGGAGAG TTCAAGCAAC CAATTGCTGT ATCTTCCATG
    CCACAACAAA TAGGTGTTCC TACCAGTTCT TTAACGCAAG TTGTTCATTC TGCTGGAAGA
    CGGTTTATAG TGAGTCCTGT ACCTGAAAGT AGGTTACGAG AATCAAAAGT TTTCACCAGT
    GACATATCAG ATCCAGTTGT GGCCTCTACA TCTCAGGCCC CTGGAATGAA CTTATCTCAC
    TCTGCTTCAT CCCTCAGTCT CCAGCAGGCT TTTTCTGAAC TGAAACATGG TCAGATGACT
    GAAGGACCCA ATACAGCACC TCCAAATTTT AATCACATGG CGGGACCAAC ATTTTCTCCC
    TTTTTGGCTA GCATTGCTGG TGTCCAAACT GTAGCAGCAT CCACACCGTC AGTATCAGTC
    CCTATAACAA GCAGTCCTCT TAATGACATT TCCACATCAG TGATGCAGTC TGAGACTGCA
    CTGCCCACTG AAAAAGGCAT CGTGGGAGTT ACCACTACCA GCACAGGTGT GGTAGCTTCA
    GGTGGTCTCA CCACAATGTC TGTGTCTGAG TCACCAACAT CTTCCAGTGC GGTTTCAAGT
    TCTACAGTAC CTGCAGTTGT CACTGTGTCC ACACCATCCC AGCCAGTTCA GGCTTCCACA
    TCTGGGAGCA TTGCTTCAAG TACAGGCAGT TTTCCTCCTG GAACATTTTC TACAACTACA
    GCTACTACAA TGGGTAGTGT GGTGGCCCCA GATGCTAAGC CTCCAACTGT ATTATTGCAA
    CAGGTAGCAA GCAATACTGC TGGGGTTGCC ATAGTGACAT CAGTCTCTAC CACCACTCCA
    TTCCCAGGCA TGGCTTCACA GCCATCCCTT CCACTTAGTA GCAGCACCTC TGCTCCTACT
    CTAGCTGAAA CAATGGTGGT TAGTGCACAC TCACTAGACA AGGCATCTCA CAGTAGTACA
    GCAGGATTGG GTTTGTCCTT TTGTGCGCCA TCCTCTTCCT CCTCCTCTGG AACAGCTGTG
    TCTACTTCTG TTTCTCAGCC TGGTATGGTT CATCCTTTGG TCATCTCATC AGCTGTAGTT
    TCTACACCTG GCCTCCCCCA ACCAGTAGTA CCTACTTCTA CACCTTTATT ACCCCAAGTA
    CCTAATATCC CACCCTTGGT ACAACCTGTT GTTAATGTGC CTGCTGTACA GCAAACACTC
    ATTCACAGTC AGCCTCAACC AGCTCTGCTT CCCAACCAGC CTCACACACA CTGTCCTGAA
    ATGGATGCTG ATACCCAATC CAAAGCTCCT GGGATTGATG ATATAAAGAC TCTGGAGGAA
    AAGCTGAGAT CTCTCTTTAG TGAACACAGC TCATCTGGAA CTCAGCATGC TTCTGTCTCC
    CTAGAGACAC CTTTAGTAGT AGAGACGACT GTCACACCGG GCATCACTAC CACTGCTGTA
    GCACCAAGTA AACTCATGAC TTCCACTACA AGTACTTGCT TACCACCAAC CAGTTTACCA
    TTAGGAGCAG CTGGCATGCC AGTTATGCCA GTAGGCACAC CTGGGCAGGT TTCTACCCCA
    GGCACACACG CTTCAGCCCC AGTCGGCACT GCAACAGGAG TAAAACCTGG AACAACTCCC
    CCAAAGCCAA CTAAGACTGT GGTGCCACCA GTGGGTACTG AACTTTCAGC CGGTACTGTA
    CCCTGTGAGC AGCTGCCACC TTTTCCTGGA CCTTCTCTAA TTCAGTCCCA GCAACCACTA
    GAAGATCTTG ATGCTCAGTT GAGAAGAACA CTTAGTCCAG AGACTATTAC AGTGGCACCT
    GCAGTTGGTC CTTTGTCTAC GATGTCTTCA ACAACAGTTA CAGAAGCAGG GACTCGGCTT
    CAAAAGGATG GCACAGAAGG TCATGTCACA GCAACTAGTT CAGGAGCTGG TGTTGTTAAG
    ATGGGGCGAT TTCAGGTTTC AGTTACAATG GATGATGCCC AGAAAGAACG TAAAAATAGG
    TCAGAAGATA CAAAGTCGGT TCATTTTGAA TCTAGCACAT CAGAGTCATC AGTTCTTTCA
    AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG CCAGAGCCTA ATGGAATTAG TATTTCTGGC
    ATCTCCTTAG ATGTCCCAGA CAGCACCCAC AAAGCACCTA CCCCAGAAGC AAAGTCAGAT
    GCAGGACAGC CTACCAAGGT GGGACGTTTT CAGGTGACAA CTACAGCCAA CAAAGTGGGT
    CGTTTCTCTG TTTCAAGAAC TGAGGACAAG GTCACAGAGC TCAAAAAAGA GGGACCAGTG
    ACCTCTCCTC CTTTCAGAGA TTCAGAACAA ACTGTTATTC CTGCTGTGAT ACCAAAGAAG
    GAGAAGCCTG AGCTGGCAGA ACCTTCCCAT TTGAATGGGC CATCTTCAGA CCTGGAGGCT
    GCCTTTCTAA GTAGGGGTAC AGAGGATGGC TCAGGTAGTC CACACTCTCC CCCTCACCTG
    TGTTCAAAGA GCCTTCCCGT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC
    TCATACATGA GTAGTGACAA TGAGTCCGAC ATTGAAGATG AAGACTTAAG GTTAGAACTA
    CGACGACTAC GAGAAAAACA TCTTAAAGAG ATTCAGGACC TGCAGAGTCG CCAGAAGCAT
    GAAATTGAAT CTTTGTATAC TAAACTGGGC AAGGTTCCCC CTGCTGTCAT TATTCCCCCA
    GCTGCTCCTC TGTCGGGGAG AAGAAGGAGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT
    CGCAGCAGCT CATTGGGCAA TAAAAGCCCA CAGCTTTCAG GCAACCTGTC TGGTCAGAGT
    GGAACTTCAG TCTTACACCC CCAACAGACC CTCCATCCCG CAGGAAACAC CCCCGAGACT
    GGGCACAACC AGCTGTTACA GCCTCTTAAG CCATCTCCCT CCAGTGACAA CCTCTATTCA
    GCCTTTACCA GTGATGGTGC CATTTCAGTA CCAAGCCTTT CTGCTCCAGG TCAAGGGACC
    AGCAGCACAA ATACCGTTGG AGGAACAGTG AGTAGCCAGG CTGCCCAGGC TCAGCCTCCT
    GCTATGACTT CCAGTAGGAA GGGCACATTC ACAGATGACC TGCACAAGTT AGTAGACAAC
    TGGGCCCGAG ATGCCATGAA TCTTTCAGGC AGGAGAGGCA GCAAAGGACA CATGAATTAT
    GAGGGCCCTG GAATGGCAAG GAAGTTCTCT GCTCCTGGGC AGCTGTGTGT TCCCATGACC
    TCAAACCTGG GTGGCTCTAC CCCCATCTCT GCAGCATCTG CTACCTCTCT AGGTCACTTC
    ACCAAATCCA TGTGTCCCCC ACAGCAGTAT GGTTTTCCAC CTGCCCCATT TGGCACTCAG
    TGGAGTGGAA CAGGTGGTCC AGCACCGCAG CCACTTGGCC AGTTCCAACC TGTAGGAACA
    GCATCCTTGC AAAATTTCAA CATCAGCAAT TTGCAGAAGT CCATCAGCAA CCCCCCAGGT
    TCCAATCTAC GGACCACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15602
    Clone ID Related Accession (Same CDS sequence) NM_198703.3 , XM_017321549.1
    Accession Version XM_017321549.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAATACCGT TGGAGGAACA
    GTGAGTAGCC AGGCTGCCCA GGCTCAGCCT CCTGCTATGA CTTCCAGTAG GAAGGGCACA
    TTCACAGATG ACCTGCACAA GTTAGTAGAC AACTGGGCCC GAGATGCCAT GAATCTTTCA
    GGCAGGAGAG GCAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC
    TCTGCTCCTG GGCAGCTGTG TGTTCCCATG ACCTCAAACC TGGGTGGCTC TACCCCCATC
    TCTGCAGCAT CTGCTACCTC TCTAGGTCAC TTCACCAAAT CCATGTGTCC CCCACAGCAG
    TATGGTTTTC CACCTGCCCC ATTTGGCACT CAGTGGAGTG GAACAGGTGG TCCAGCACCG
    CAGCCACTTG GCCAGTTCCA ACCTGTAGGA ACAGCATCCT TGCAAAATTT CAACATCAGC
    AATTTGCAGA AGTCCATCAG CAACCCCCCA GGTTCCAATC TACGGACCAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177038.1
    CDS885..8018
    Translation

    Target ORF information:

    RefSeq Version XM_017321549.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321549.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGC TTCCAGTTTC CCAGACAGTA GCAACTGTCC AAGGTGAACC TCACATCCCA
    GTTTCAACAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCTATGGGA
    CAGCCAATCC CTACTTCTTT ACTCCCACAG TATCCTGTCT CTCAAATTCC CATATCGACT
    CCTCATGTGT CCACGGCGCA GACAGGTTTC TCATCCGTTC CTATAACAAT GGCTGCTGGC
    ATTAACCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CATCTTCAAT CCCAGGGGGA
    TCACCTGTAG TTCCTAATCA GCTTCCAACT CTTCTGCAGC CTGTGAATCA GCTGCAGAGT
    CAGGTTCACC CACAGCTTCT ACAGCCAACA ACAGTTCAGT CCATAGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGG TCCACTTCCC TCTGGAGATG TTTTGTACCA GGGCTTCCCA
    TCTCGACTGC CACCACAATA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTAGCC CCTCCTTCTA TGCCGACAGA AGCATTGGCC
    ACCCAAGGTT ACTTTCCTAC AGTAGTGCAG CCTTATGTGG AATCAACTCC TTTGGTTCCT
    ATGGGCAGTG TAGGAGGCCA GGTTCAAGTG TCCCAACCAG CAGTGAGTTT AACGCAACAA
    CCCCCAACTA CATCCTCCCA GCAAGCAGTT CTGGAGAGTA CTCAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAGCCTGT TCCATTGGTT
    ACCTCTGCAG ACAGTGCACA TTCAGACGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCATCAT CCAGCGGGAG GCATGAAGGG AGGACAACAA AACGGCATTA TAGAAAATCC
    GTAAGAAGTC GCTCTCGGCA TGAAAAGACT TCTCGCCCAA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTAGAGA CTCATAATCG GAAGATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCAAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGTGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TGCAAGGAAA GGATGACTAT GGCTTCCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACGC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTACCTGAA
    AGTAGGTTAC GAGAATCAAA AGTTTTCACC AGTGACATAT CAGATCCAGT TGTGGCCTCT
    ACATCTCAGG CCCCTGGAAT GAACTTATCT CACTCTGCTT CATCCCTCAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACA TGGCGGGACC AACATTTTCT CCCTTTTTGG CTAGCATTGC TGGTGTCCAA
    ACTGTAGCAG CATCCACACC GTCAGTATCA GTCCCTATAA CAAGCAGTCC TCTTAATGAC
    ATTTCCACAT CAGTGATGCA GTCTGAGACT GCACTGCCCA CTGAAAAAGG CATCGTGGGA
    GTTACCACTA CCAGCACAGG TGTGGTAGCT TCAGGTGGTC TCACCACAAT GTCTGTGTCT
    GAGTCACCAA CATCTTCCAG TGCGGTTTCA AGTTCTACAG TACCTGCAGT TGTCACTGTG
    TCCACACCAT CCCAGCCAGT TCAGGCTTCC ACATCTGGGA GCATTGCTTC AAGTACAGGC
    AGTTTTCCTC CTGGAACATT TTCTACAACT ACAGCTACTA CAATGGGTAG TGTGGTGGCC
    CCAGATGCTA AGCCTCCAAC TGTATTATTG CAACAGGTAG CAAGCAATAC TGCTGGGGTT
    GCCATAGTGA CATCAGTCTC TACCACCACT CCATTCCCAG GCATGGCTTC ACAGCCATCC
    CTTCCACTTA GTAGCAGCAC CTCTGCTCCT ACTCTAGCTG AAACAATGGT GGTTAGTGCA
    CACTCACTAG ACAAGGCATC TCACAGTAGT ACAGCAGGAT TGGGTTTGTC CTTTTGTGCG
    CCATCCTCTT CCTCCTCCTC TGGAACAGCT GTGTCTACTT CTGTTTCTCA GCCTGGTATG
    GTTCATCCTT TGGTCATCTC ATCAGCTGTA GTTTCTACAC CTGGCCTCCC CCAACCAGTA
    GTACCTACTT CTACACCTTT ATTACCCCAA GTACCTAATA TCCCACCCTT GGTACAACCT
    GTTGTTAATG TGCCTGCTGT ACAGCAAACA CTCATTCACA GTCAGCCTCA ACCAGCTCTG
    CTTCCCAACC AGCCTCACAC ACACTGTCCT GAAATGGATG CTGATACCCA ATCCAAAGCT
    CCTGGGATTG ATGATATAAA GACTCTGGAG GAAAAGCTGA GATCTCTCTT TAGTGAACAC
    AGCTCATCTG GAACTCAGCA TGCTTCTGTC TCCCTAGAGA CACCTTTAGT AGTAGAGACG
    ACTGTCACAC CGGGCATCAC TACCACTGCT GTAGCACCAA GTAAACTCAT GACTTCCACT
    ACAAGTACTT GCTTACCACC AACCAGTTTA CCATTAGGAG CAGCTGGCAT GCCAGTTATG
    CCAGTAGGCA CACCTGGGCA GGTTTCTACC CCAGGCACAC ACGCTTCAGC CCCAGTCGGC
    ACTGCAACAG GAGTAAAACC TGGAACAACT CCCCCAAAGC CAACTAAGAC TGTGGTGCCA
    CCAGTGGGTA CTGAACTTTC AGCCGGTACT GTACCCTGTG AGCAGCTGCC ACCTTTTCCT
    GGACCTTCTC TAATTCAGTC CCAGCAACCA CTAGAAGATC TTGATGCTCA GTTGAGAAGA
    ACACTTAGTC CAGAGACTAT TACAGTGGCA CCTGCAGTTG GTCCTTTGTC TACGATGTCT
    TCAACAACAG TTACAGAAGC AGGGACTCGG CTTCAAAAGG ATGGCACAGA AGGTCATGTC
    ACAGCAACTA GTTCAGGAGC TGGTGTTGTT AAGATGGGGC GATTTCAGGT TTCAGTTACA
    ATGGATGATG CCCAGAAAGA ACGTAAAAAT AGGTCAGAAG ATACAAAGTC GGTTCATTTT
    GAATCTAGCA CATCAGAGTC ATCAGTTCTT TCAAGTAGTA GTCCAGAGAG TACCCTGGTA
    AAGCCAGAGC CTAATGGAAT TAGTATTTCT GGCATCTCCT TAGATGTCCC AGACAGCACC
    CACAAAGCAC CTACCCCAGA AGCAAAGTCA GATGCAGGAC AGCCTACCAA GGTGGGACGT
    TTTCAGGTGA CAACTACAGC CAACAAAGTG GGTCGTTTCT CTGTTTCAAG AACTGAGGAC
    AAGGTCACAG AGCTCAAAAA AGAGGGACCA GTGACCTCTC CTCCTTTCAG AGATTCAGAA
    CAAACTGTTA TTCCTGCTGT GATACCAAAG AAGGAGAAGC CTGAGCTGGC AGAACCTTCC
    CATTTGAATG GGCCATCTTC AGACCTGGAG GCTGCCTTTC TAAGTAGGGG TACAGAGGAT
    GGCTCAGGTA GTCCACACTC TCCCCCTCAC CTGTGTTCAA AGAGCCTTCC CGTCCAGAAT
    CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCATACA TGAGTAGTGA CAATGAGTCC
    GACATTGAAG ATGAAGACTT AAGGTTAGAA CTACGACGAC TACGAGAAAA ACATCTTAAA
    GAGATTCAGG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACTAAACTG
    GGCAAGGTTC CCCCTGCTGT CATTATTCCC CCAGCTGCTC CTCTGTCGGG GAGAAGAAGG
    AGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GCTCATTGGG CAATAAAAGC
    CCACAGCTTT CAGGCAACCT GTCTGGTCAG AGTGGAACTT CAGTCTTACA CCCCCAACAG
    ACCCTCCATC CCGCAGGAAA CACCCCCGAG ACTGGGCACA ACCAGCTGTT ACAGCCTCTT
    AAGCCATCTC CCTCCAGTGA CAACCTCTAT TCAGCCTTTA CCAGTGATGG TGCCATTTCA
    GTACCAAGCC TTTCTGCTCC AGGTCAAGGG ACCAGCAGCA CAAATACCGT TGGAGGAACA
    GTGAGTAGCC AGGCTGCCCA GGCTCAGCCT CCTGCTATGA CTTCCAGTAG GAAGGGCACA
    TTCACAGATG ACCTGCACAA GTTAGTAGAC AACTGGGCCC GAGATGCCAT GAATCTTTCA
    GGCAGGAGAG GCAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTC
    TCTGCTCCTG GGCAGCTGTG TGTTCCCATG ACCTCAAACC TGGGTGGCTC TACCCCCATC
    TCTGCAGCAT CTGCTACCTC TCTAGGTCAC TTCACCAAAT CCATGTGTCC CCCACAGCAG
    TATGGTTTTC CACCTGCCCC ATTTGGCACT CAGTGGAGTG GAACAGGTGG TCCAGCACCG
    CAGCCACTTG GCCAGTTCCA ACCTGTAGGA ACAGCATCCT TGCAAAATTT CAACATCAGC
    AATTTGCAGA AGTCCATCAG CAACCCCCCA GGTTCCAATC TACGGACCAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15715
    Clone ID Related Accession (Same CDS sequence) XM_017321557.1 , NM_001185020.1
    Accession Version XM_017321557.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGA GTACTCAGGG AGTCTCTCAG GCTGCTCCTC CAGAACAAAC TCCAATAACA
    CAGTCACAAC CTACTCAGCC TGTTCCATTG GTTACCTCTG CAGACAGTGC ACATTCAGAC
    GTTGCTTCTG GTATGAGTGA TGGCAATGAG AATGCTCCAT CATCCAGCGG GAGGCATGAA
    GGGAGGACAA CAAAACGGCA TTATAGAAAA TCCGTAAGAA GTCGCTCTCG GCATGAAAAG
    ACTTCTCGCC CAAAACTGAG GATTTTAAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TCGGAAGATG GTTACATTTA AATTTGACCT AGATGGTGAC
    AACCCTGAAG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCTTTTG TGGCTCAAGT GCGGGAAATT ATTGAAAAGG CTGATGAAAT GTTAAGTGAG
    GATGTCAGTG TGGAGCCTGA GGGTGACCAG GGATTGGAGA GTCTGCAAGG AAAGGATGAC
    TATGGCTTCC CAGGTTCTCA GAAATTAGAA GGAGAGTTCA AGCAACCAAT TGCTGTATCT
    TCCATGCCAC AACAAATAGG TGTTCCTACC AGTTCTTTAA CGCAAGTTGT TCATTCTGCT
    GGAAGACGGT TTATAGTGAG TCCTGTACCT GAAAGTAGGT TACGAGAATC AAAAGTTTTC
    ACCAGTGACA TATCAGATCC AGTTGTGGCC TCTACATCTC AGGCCCCTGG AATGAACTTA
    TCTCACTCTG CTTCATCCCT CAGTCTCCAG CAGGCTTTTT CTGAACTGAA ACATGGTCAG
    ATGACTGAAG GACCCAATAC AGCACCTCCA AATTTTAATC ACATGGCGGG ACCAACATTT
    TCTCCCTTTT TGGCTAGCAT TGCTGGTGTC CAAACTGTAG CAGCATCCAC ACCGTCAGTA
    TCAGTCCCTA TAACAAGCAG TCCTCTTAAT GACATTTCCA CATCAGTGAT GCAGTCTGAG
    ACTGCACTGC CCACTGAAAA AGGCATCGTG GGAGTTACCA CTACCAGCAC AGGTGTGGTA
    GCTTCAGGTG GTCTCACCAC AATGTCTGTG TCTGAGTCAC CAACATCTTC CAGTGCGGTT
    TCAAGTTCTA CAGTACCTGC AGTTGTCACT GTGTCCACAC CATCCCAGCC AGTTCAGGCT
    TCCACATCTG GGAGCATTGC TTCAAGTACA GGCAGTTTTC CTCCTGGAAC ATTTTCTACA
    ACTACAGCTA CTACAATGGG TAGTGTGGTG GCCCCAGATG CTAAGCCTCC AACTGTATTA
    TTGCAACAGG TAGCAAGCAA TACTGCTGGG GTTGCCATAG TGACATCAGT CTCTACCACC
    ACTCCATTCC CAGGCATGGC TTCACAGCCA TCCCTTCCAC TTAGTAGCAG CACCTCTGCT
    CCTACTCTAG CTGAAACAAT GGTGGTTAGT GCACACTCAC TAGACAAGGC ATCTCACAGT
    AGTACAGCAG GATTGGGTTT GTCCTTTTGT GCGCCATCCT CTTCCTCCTC CTCTGGAACA
    GCTGTGTCTA CTTCTGTTTC TCAGCCTGGT ATGGTTCATC CTTTGGTCAT CTCATCAGCT
    GTAGTTTCTA CACCTGGCCT CCCCCAACCA GTAGTACCTA CTTCTACACC TTTATTACCC
    CAAGTACCTA ATATCCCACC CTTGGTACAA CCTGTTGTTA ATGTGCCTGC TGTACAGCAA
    ACACTCATTC ACAGTCAGCC TCAACCAGCT CTGCTTCCCA ACCAGCCTCA CACACACTGT
    CCTGAAATGG ATGCTGATAC CCAATCCAAA GCTCCTGGGA TTGATGATAT AAAGACTCTG
    GAGGAAAAGC TGAGATCTCT CTTTAGTGAA CACAGCTCAT CTGGAACTCA GCATGCTTCT
    GTCTCCCTAG AGACACCTTT AGTAGTAGAG ACGACTGTCA CACCGGGCAT CACTACCACT
    GCTGTAGCAC CAAGTAAACT CATGACTTCC ACTACAAGTA CTTGCTTACC ACCAACCAGT
    TTACCATTAG GAGCAGCTGG CATGCCAGTT ATGCCAGTAG GCACACCTGG GCAGGTTTCT
    ACCCCAGGCA CACACGCTTC AGCCCCAGTC GGCACTGCAA CAGGAGTAAA ACCTGGAACA
    ACTCCCCCAA AGCCAACTAA GACTGTGGTG CCACCAGTGG GTACTGAACT TTCAGCCGGT
    ACTGTACCCT GTGAGCAGCT GCCACCTTTT CCTGGACCTT CTCTAATTCA GTCCCAGCAA
    CCACTAGAAG ATCTTGATGC TCAGTTGAGA AGAACACTTA GTCCAGAGAC TATTACAGTG
    GCACCTGCAG TTGGTCCTTT GTCTACGATG TCTTCAACAA CAGTTACAGA AGCAGGGACT
    CGGCTTCAAA AGGATGGCAC AGAAGGTCAT GTCACAGCAA CTAGTTCAGG AGCTGGTGTT
    GTTAAGATGG GGCGATTTCA GGTTTCAGTT ACAATGGATG ATGCCCAGAA AGAACGTAAA
    AATAGGTCAG AAGATACAAA GTCGGTTCAT TTTGAATCTA GCACATCAGA GTCATCAGTT
    CTTTCAAGTA GTAGTCCAGA GAGTACCCTG GTAAAGCCAG AGCCTAATGG AATTAGTATT
    TCTGGCATCT CCTTAGATGT CCCAGACAGC ACCCACAAAG CACCTACCCC AGAAGCAAAG
    TCAGATGCAG GACAGCCTAC CAAGGTGGGA CGTTTTCAGG TGACAACTAC AGCCAACAAA
    GTGGGTCGTT TCTCTGTTTC AAGAACTGAG GACAAGGTCA CAGAGCTCAA AAAAGAGGGA
    CCAGTGACCT CTCCTCCTTT CAGAGATTCA GAACAAACTG TTATTCCTGC TGTGATACCA
    AAGAAGGAGA AGCCTGAGCT GGCAGAACCT TCCCATTTGA ATGGGCCATC TTCAGACCTG
    GAGGCTGCCT TTCTAAGTAG GGGTACAGAG GATGGCTCAG GTAGTCCACA CTCTCCCCCT
    CACCTGTGTT CAAAGAGCCT TCCCGTCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTC
    AACTCCTCAT ACATGAGTAG TGACAATGAG TCCGACATTG AAGATGAAGA CTTAAGGTTA
    GAACTACGAC GACTACGAGA AAAACATCTT AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACTAAA CTGGGCAAGG TTCCCCCTGC TGTCATTATT
    CCCCCAGCTG CTCCTCTGTC GGGGAGAAGA AGGAGACCCA CTAAAAGCAA AGGCAGCAAG
    TCTAGTCGCA GCAGCTCATT GGGCAATAAA AGCCCACAGC TTTCAGGCAA CCTGTCTGGT
    CAGAGTGGAA CTTCAGTCTT ACACCCCCAA CAGACCCTCC ATCCCGCAGG AAACACCCCC
    GAGACTGGGC ACAACCAGCT GTTACAGCCT CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TTACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGGACCAGCA GCACAAATAC CGTTGGAGGA ACAGTGAGTA GCCAGGCTGC CCAGGCTCAG
    CCTCCTGCTA TGACTTCCAG TAGGAAGGGC ACATTCACAG ATGACCTGCA CAAGTTAGTA
    GACAACTGGG CCCGAGATGC CATGAATCTT TCAGGCAGGA GAGGCAGCAA AGGACACATG
    AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCTC CTGGGCAGCT GTGTGTTCCC
    ATGACCTCAA ACCTGGGTGG CTCTACCCCC ATCTCTGCAG CATCTGCTAC CTCTCTAGGT
    CACTTCACCA AATCCATGTG TCCCCCACAG CAGTATGGTT TTCCACCTGC CCCATTTGGC
    ACTCAGTGGA GTGGAACAGG TGGTCCAGCA CCGCAGCCAC TTGGCCAGTT CCAACCTGTA
    GGAACAGCAT CCTTGCAAAA TTTCAACATC AGCAATTTGC AGAAGTCCAT CAGCAACCCC
    CCAGGTTCCA ATCTACGGAC CACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177046.1
    CDS885..7271
    Translation

    Target ORF information:

    RefSeq Version XM_017321557.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGA GTACTCAGGG AGTCTCTCAG GCTGCTCCTC CAGAACAAAC TCCAATAACA
    CAGTCACAAC CTACTCAGCC TGTTCCATTG GTTACCTCTG CAGACAGTGC ACATTCAGAC
    GTTGCTTCTG GTATGAGTGA TGGCAATGAG AATGCTCCAT CATCCAGCGG GAGGCATGAA
    GGGAGGACAA CAAAACGGCA TTATAGAAAA TCCGTAAGAA GTCGCTCTCG GCATGAAAAG
    ACTTCTCGCC CAAAACTGAG GATTTTAAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TCGGAAGATG GTTACATTTA AATTTGACCT AGATGGTGAC
    AACCCTGAAG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCTTTTG TGGCTCAAGT GCGGGAAATT ATTGAAAAGG CTGATGAAAT GTTAAGTGAG
    GATGTCAGTG TGGAGCCTGA GGGTGACCAG GGATTGGAGA GTCTGCAAGG AAAGGATGAC
    TATGGCTTCC CAGGTTCTCA GAAATTAGAA GGAGAGTTCA AGCAACCAAT TGCTGTATCT
    TCCATGCCAC AACAAATAGG TGTTCCTACC AGTTCTTTAA CGCAAGTTGT TCATTCTGCT
    GGAAGACGGT TTATAGTGAG TCCTGTACCT GAAAGTAGGT TACGAGAATC AAAAGTTTTC
    ACCAGTGACA TATCAGATCC AGTTGTGGCC TCTACATCTC AGGCCCCTGG AATGAACTTA
    TCTCACTCTG CTTCATCCCT CAGTCTCCAG CAGGCTTTTT CTGAACTGAA ACATGGTCAG
    ATGACTGAAG GACCCAATAC AGCACCTCCA AATTTTAATC ACATGGCGGG ACCAACATTT
    TCTCCCTTTT TGGCTAGCAT TGCTGGTGTC CAAACTGTAG CAGCATCCAC ACCGTCAGTA
    TCAGTCCCTA TAACAAGCAG TCCTCTTAAT GACATTTCCA CATCAGTGAT GCAGTCTGAG
    ACTGCACTGC CCACTGAAAA AGGCATCGTG GGAGTTACCA CTACCAGCAC AGGTGTGGTA
    GCTTCAGGTG GTCTCACCAC AATGTCTGTG TCTGAGTCAC CAACATCTTC CAGTGCGGTT
    TCAAGTTCTA CAGTACCTGC AGTTGTCACT GTGTCCACAC CATCCCAGCC AGTTCAGGCT
    TCCACATCTG GGAGCATTGC TTCAAGTACA GGCAGTTTTC CTCCTGGAAC ATTTTCTACA
    ACTACAGCTA CTACAATGGG TAGTGTGGTG GCCCCAGATG CTAAGCCTCC AACTGTATTA
    TTGCAACAGG TAGCAAGCAA TACTGCTGGG GTTGCCATAG TGACATCAGT CTCTACCACC
    ACTCCATTCC CAGGCATGGC TTCACAGCCA TCCCTTCCAC TTAGTAGCAG CACCTCTGCT
    CCTACTCTAG CTGAAACAAT GGTGGTTAGT GCACACTCAC TAGACAAGGC ATCTCACAGT
    AGTACAGCAG GATTGGGTTT GTCCTTTTGT GCGCCATCCT CTTCCTCCTC CTCTGGAACA
    GCTGTGTCTA CTTCTGTTTC TCAGCCTGGT ATGGTTCATC CTTTGGTCAT CTCATCAGCT
    GTAGTTTCTA CACCTGGCCT CCCCCAACCA GTAGTACCTA CTTCTACACC TTTATTACCC
    CAAGTACCTA ATATCCCACC CTTGGTACAA CCTGTTGTTA ATGTGCCTGC TGTACAGCAA
    ACACTCATTC ACAGTCAGCC TCAACCAGCT CTGCTTCCCA ACCAGCCTCA CACACACTGT
    CCTGAAATGG ATGCTGATAC CCAATCCAAA GCTCCTGGGA TTGATGATAT AAAGACTCTG
    GAGGAAAAGC TGAGATCTCT CTTTAGTGAA CACAGCTCAT CTGGAACTCA GCATGCTTCT
    GTCTCCCTAG AGACACCTTT AGTAGTAGAG ACGACTGTCA CACCGGGCAT CACTACCACT
    GCTGTAGCAC CAAGTAAACT CATGACTTCC ACTACAAGTA CTTGCTTACC ACCAACCAGT
    TTACCATTAG GAGCAGCTGG CATGCCAGTT ATGCCAGTAG GCACACCTGG GCAGGTTTCT
    ACCCCAGGCA CACACGCTTC AGCCCCAGTC GGCACTGCAA CAGGAGTAAA ACCTGGAACA
    ACTCCCCCAA AGCCAACTAA GACTGTGGTG CCACCAGTGG GTACTGAACT TTCAGCCGGT
    ACTGTACCCT GTGAGCAGCT GCCACCTTTT CCTGGACCTT CTCTAATTCA GTCCCAGCAA
    CCACTAGAAG ATCTTGATGC TCAGTTGAGA AGAACACTTA GTCCAGAGAC TATTACAGTG
    GCACCTGCAG TTGGTCCTTT GTCTACGATG TCTTCAACAA CAGTTACAGA AGCAGGGACT
    CGGCTTCAAA AGGATGGCAC AGAAGGTCAT GTCACAGCAA CTAGTTCAGG AGCTGGTGTT
    GTTAAGATGG GGCGATTTCA GGTTTCAGTT ACAATGGATG ATGCCCAGAA AGAACGTAAA
    AATAGGTCAG AAGATACAAA GTCGGTTCAT TTTGAATCTA GCACATCAGA GTCATCAGTT
    CTTTCAAGTA GTAGTCCAGA GAGTACCCTG GTAAAGCCAG AGCCTAATGG AATTAGTATT
    TCTGGCATCT CCTTAGATGT CCCAGACAGC ACCCACAAAG CACCTACCCC AGAAGCAAAG
    TCAGATGCAG GACAGCCTAC CAAGGTGGGA CGTTTTCAGG TGACAACTAC AGCCAACAAA
    GTGGGTCGTT TCTCTGTTTC AAGAACTGAG GACAAGGTCA CAGAGCTCAA AAAAGAGGGA
    CCAGTGACCT CTCCTCCTTT CAGAGATTCA GAACAAACTG TTATTCCTGC TGTGATACCA
    AAGAAGGAGA AGCCTGAGCT GGCAGAACCT TCCCATTTGA ATGGGCCATC TTCAGACCTG
    GAGGCTGCCT TTCTAAGTAG GGGTACAGAG GATGGCTCAG GTAGTCCACA CTCTCCCCCT
    CACCTGTGTT CAAAGAGCCT TCCCGTCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTC
    AACTCCTCAT ACATGAGTAG TGACAATGAG TCCGACATTG AAGATGAAGA CTTAAGGTTA
    GAACTACGAC GACTACGAGA AAAACATCTT AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACTAAA CTGGGCAAGG TTCCCCCTGC TGTCATTATT
    CCCCCAGCTG CTCCTCTGTC GGGGAGAAGA AGGAGACCCA CTAAAAGCAA AGGCAGCAAG
    TCTAGTCGCA GCAGCTCATT GGGCAATAAA AGCCCACAGC TTTCAGGCAA CCTGTCTGGT
    CAGAGTGGAA CTTCAGTCTT ACACCCCCAA CAGACCCTCC ATCCCGCAGG AAACACCCCC
    GAGACTGGGC ACAACCAGCT GTTACAGCCT CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TTACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGGACCAGCA GCACAAATAC CGTTGGAGGA ACAGTGAGTA GCCAGGCTGC CCAGGCTCAG
    CCTCCTGCTA TGACTTCCAG TAGGAAGGGC ACATTCACAG ATGACCTGCA CAAGTTAGTA
    GACAACTGGG CCCGAGATGC CATGAATCTT TCAGGCAGGA GAGGCAGCAA AGGACACATG
    AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCTC CTGGGCAGCT GTGTGTTCCC
    ATGACCTCAA ACCTGGGTGG CTCTACCCCC ATCTCTGCAG CATCTGCTAC CTCTCTAGGT
    CACTTCACCA AATCCATGTG TCCCCCACAG CAGTATGGTT TTCCACCTGC CCCATTTGGC
    ACTCAGTGGA GTGGAACAGG TGGTCCAGCA CCGCAGCCAC TTGGCCAGTT CCAACCTGTA
    GGAACAGCAT CCTTGCAAAA TTTCAACATC AGCAATTTGC AGAAGTCCAT CAGCAACCCC
    CCAGGTTCCA ATCTACGGAC CACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15743
    Clone ID Related Accession (Same CDS sequence) NM_001185021.1 , XM_017321554.1
    Accession Version XM_017321554.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGG GCTTCCCATC TCGACTGCCA CCACAATACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTAGCCCC TCCTTCTATG
    CCGACAGAAG CATTGGCCAC CCAAGGTTAC TTTCCTACAG TAGTGCAGCC TTATGTGGAA
    TCAACTCCTT TGGTTCCTAT GGGCAGTGTA GGAGGCCAGG TTCAAGTGTC CCAACCAGCA
    GTGAGTTTAA CGCAACAACC CCCAACTACA TCCTCCCAGC AAGCAGTTCT GGAGAGTACT
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAGCCTGTTC CATTGGTTAC CTCTGCAGAC AGTGCACATT CAGACGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCATCATCC AGCGGGAGGC ATGAAGGGAG GACAACAAAA
    CGGCATTATA GAAAATCCGT AAGAAGTCGC TCTCGGCATG AAAAGACTTC TCGCCCAAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTAGAGACT
    CATAATCGGA AGATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GTGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTG CAAGGAAAGG ATGACTATGG CTTCCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACGCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TACCTGAAAG TAGGTTACGA GAATCAAAAG TTTTCACCAG TGACATATCA
    GATCCAGTTG TGGCCTCTAC ATCTCAGGCC CCTGGAATGA ACTTATCTCA CTCTGCTTCA
    TCCCTCAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACATG GCGGGACCAA CATTTTCTCC CTTTTTGGCT
    AGCATTGCTG GTGTCCAAAC TGTAGCAGCA TCCACACCGT CAGTATCAGT CCCTATAACA
    AGCAGTCCTC TTAATGACAT TTCCACATCA GTGATGCAGT CTGAGACTGC ACTGCCCACT
    GAAAAAGGCA TCGTGGGAGT TACCACTACC AGCACAGGTG TGGTAGCTTC AGGTGGTCTC
    ACCACAATGT CTGTGTCTGA GTCACCAACA TCTTCCAGTG CGGTTTCAAG TTCTACAGTA
    CCTGCAGTTG TCACTGTGTC CACACCATCC CAGCCAGTTC AGGCTTCCAC ATCTGGGAGC
    ATTGCTTCAA GTACAGGCAG TTTTCCTCCT GGAACATTTT CTACAACTAC AGCTACTACA
    ATGGGTAGTG TGGTGGCCCC AGATGCTAAG CCTCCAACTG TATTATTGCA ACAGGTAGCA
    AGCAATACTG CTGGGGTTGC CATAGTGACA TCAGTCTCTA CCACCACTCC ATTCCCAGGC
    ATGGCTTCAC AGCCATCCCT TCCACTTAGT AGCAGCACCT CTGCTCCTAC TCTAGCTGAA
    ACAATGGTGG TTAGTGCACA CTCACTAGAC AAGGCATCTC ACAGTAGTAC AGCAGGATTG
    GGTTTGTCCT TTTGTGCGCC ATCCTCTTCC TCCTCCTCTG GAACAGCTGT GTCTACTTCT
    GTTTCTCAGC CTGGTATGGT TCATCCTTTG GTCATCTCAT CAGCTGTAGT TTCTACACCT
    GGCCTCCCCC AACCAGTAGT ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAATATC
    CCACCCTTGG TACAACCTGT TGTTAATGTG CCTGCTGTAC AGCAAACACT CATTCACAGT
    CAGCCTCAAC CAGCTCTGCT TCCCAACCAG CCTCACACAC ACTGTCCTGA AATGGATGCT
    GATACCCAAT CCAAAGCTCC TGGGATTGAT GATATAAAGA CTCTGGAGGA AAAGCTGAGA
    TCTCTCTTTA GTGAACACAG CTCATCTGGA ACTCAGCATG CTTCTGTCTC CCTAGAGACA
    CCTTTAGTAG TAGAGACGAC TGTCACACCG GGCATCACTA CCACTGCTGT AGCACCAAGT
    AAACTCATGA CTTCCACTAC AAGTACTTGC TTACCACCAA CCAGTTTACC ATTAGGAGCA
    GCTGGCATGC CAGTTATGCC AGTAGGCACA CCTGGGCAGG TTTCTACCCC AGGCACACAC
    GCTTCAGCCC CAGTCGGCAC TGCAACAGGA GTAAAACCTG GAACAACTCC CCCAAAGCCA
    ACTAAGACTG TGGTGCCACC AGTGGGTACT GAACTTTCAG CCGGTACTGT ACCCTGTGAG
    CAGCTGCCAC CTTTTCCTGG ACCTTCTCTA ATTCAGCCTT TGTCTACGAT GTCTTCAACA
    ACAGTTACAG AAGCAGGGAC TCGGCTTCAA AAGGATGGCA CAGAAGGTCA TGTCACAGCA
    ACTAGTTCAG GAGCTGGTGT TGTTAAGATG GGGCGATTTC AGGTTTCAGT TACAATGGAT
    GATGCCCAGA AAGAACGTAA AAATAGGTCA GAAGATACAA AGTCGGTTCA TTTTGAATCT
    AGCACATCAG AGTCATCAGT TCTTTCAAGT AGTAGTCCAG AGAGTACCCT GGTAAAGCCA
    GAGCCTAATG GAATTAGTAT TTCTGGCATC TCCTTAGATG TCCCAGACAG CACCCACAAA
    GCACCTACCC CAGAAGCAAA GTCAGATGCA GGACAGCCTA CCAAGGTGGG ACGTTTTCAG
    GTGACAACTA CAGCCAACAA AGTGGGTCGT TTCTCTGTTT CAAGAACTGA GGACAAGGTC
    ACAGAGCTCA AAAAAGAGGG ACCAGTGACC TCTCCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGTGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATTTG
    AATGGGCCAT CTTCAGACCT GGAGGCTGCC TTTCTAAGTA GGGGTACAGA GGATGGCTCA
    GGTAGTCCAC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCGTCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCA TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTACGA CGACTACGAG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACTAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CGGGGAGAAG AAGGAGACCC
    ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC AGCAGCTCAT TGGGCAATAA AAGCCCACAG
    CTTTCAGGCA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TACACCCCCA ACAGACCCTC
    CATCCCGCAG GAAACACCCC CGAGACTGGG CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGGACCAGC AGCACAAATA CCGTTGGAGG AACAGTGAGT
    AGCCAGGCTG CCCAGGCTCA GCCTCCTGCT ATGACTTCCA GTAGGAAGGG CACATTCACA
    GATGACCTGC ACAAGTTAGT AGACAACTGG GCCCGAGATG CCATGAATCT TTCAGGCAGG
    AGAGGCAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT
    CCTGGGCAGC TGTGTGTTCC CATGACCTCA AACCTGGGTG GCTCTACCCC CATCTCTGCA
    GCATCTGCTA CCTCTCTAGG TCACTTCACC AAATCCATGT GTCCCCCACA GCAGTATGGT
    TTTCCACCTG CCCCATTTGG CACTCAGTGG AGTGGAACAG GTGGTCCAGC ACCGCAGCCA
    CTTGGCCAGT TCCAACCTGT AGGAACAGCA TCCTTGCAAA ATTTCAACAT CAGCAATTTG
    CAGAAGTCCA TCAGCAACCC CCCAGGTTCC AATCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177043.1
    CDS885..7472
    Translation

    Target ORF information:

    RefSeq Version XM_017321554.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321554.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCAAC TGTTCCTTCT CAACAGGCAG TACAGTATTC ACTTCCACAA
    GCAGCATCTT CCAGTGAAGG TACTACTGCA CAGCCAGTGA GTCAGCCTCA AGTGTCAGCT
    GGAACACAGG GCTTCCCATC TCGACTGCCA CCACAATACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTAGCCCC TCCTTCTATG
    CCGACAGAAG CATTGGCCAC CCAAGGTTAC TTTCCTACAG TAGTGCAGCC TTATGTGGAA
    TCAACTCCTT TGGTTCCTAT GGGCAGTGTA GGAGGCCAGG TTCAAGTGTC CCAACCAGCA
    GTGAGTTTAA CGCAACAACC CCCAACTACA TCCTCCCAGC AAGCAGTTCT GGAGAGTACT
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAGCCTGTTC CATTGGTTAC CTCTGCAGAC AGTGCACATT CAGACGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCATCATCC AGCGGGAGGC ATGAAGGGAG GACAACAAAA
    CGGCATTATA GAAAATCCGT AAGAAGTCGC TCTCGGCATG AAAAGACTTC TCGCCCAAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTAGAGACT
    CATAATCGGA AGATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GTGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTG CAAGGAAAGG ATGACTATGG CTTCCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACGCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA
    GTGAGTCCTG TACCTGAAAG TAGGTTACGA GAATCAAAAG TTTTCACCAG TGACATATCA
    GATCCAGTTG TGGCCTCTAC ATCTCAGGCC CCTGGAATGA ACTTATCTCA CTCTGCTTCA
    TCCCTCAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACATG GCGGGACCAA CATTTTCTCC CTTTTTGGCT
    AGCATTGCTG GTGTCCAAAC TGTAGCAGCA TCCACACCGT CAGTATCAGT CCCTATAACA
    AGCAGTCCTC TTAATGACAT TTCCACATCA GTGATGCAGT CTGAGACTGC ACTGCCCACT
    GAAAAAGGCA TCGTGGGAGT TACCACTACC AGCACAGGTG TGGTAGCTTC AGGTGGTCTC
    ACCACAATGT CTGTGTCTGA GTCACCAACA TCTTCCAGTG CGGTTTCAAG TTCTACAGTA
    CCTGCAGTTG TCACTGTGTC CACACCATCC CAGCCAGTTC AGGCTTCCAC ATCTGGGAGC
    ATTGCTTCAA GTACAGGCAG TTTTCCTCCT GGAACATTTT CTACAACTAC AGCTACTACA
    ATGGGTAGTG TGGTGGCCCC AGATGCTAAG CCTCCAACTG TATTATTGCA ACAGGTAGCA
    AGCAATACTG CTGGGGTTGC CATAGTGACA TCAGTCTCTA CCACCACTCC ATTCCCAGGC
    ATGGCTTCAC AGCCATCCCT TCCACTTAGT AGCAGCACCT CTGCTCCTAC TCTAGCTGAA
    ACAATGGTGG TTAGTGCACA CTCACTAGAC AAGGCATCTC ACAGTAGTAC AGCAGGATTG
    GGTTTGTCCT TTTGTGCGCC ATCCTCTTCC TCCTCCTCTG GAACAGCTGT GTCTACTTCT
    GTTTCTCAGC CTGGTATGGT TCATCCTTTG GTCATCTCAT CAGCTGTAGT TTCTACACCT
    GGCCTCCCCC AACCAGTAGT ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAATATC
    CCACCCTTGG TACAACCTGT TGTTAATGTG CCTGCTGTAC AGCAAACACT CATTCACAGT
    CAGCCTCAAC CAGCTCTGCT TCCCAACCAG CCTCACACAC ACTGTCCTGA AATGGATGCT
    GATACCCAAT CCAAAGCTCC TGGGATTGAT GATATAAAGA CTCTGGAGGA AAAGCTGAGA
    TCTCTCTTTA GTGAACACAG CTCATCTGGA ACTCAGCATG CTTCTGTCTC CCTAGAGACA
    CCTTTAGTAG TAGAGACGAC TGTCACACCG GGCATCACTA CCACTGCTGT AGCACCAAGT
    AAACTCATGA CTTCCACTAC AAGTACTTGC TTACCACCAA CCAGTTTACC ATTAGGAGCA
    GCTGGCATGC CAGTTATGCC AGTAGGCACA CCTGGGCAGG TTTCTACCCC AGGCACACAC
    GCTTCAGCCC CAGTCGGCAC TGCAACAGGA GTAAAACCTG GAACAACTCC CCCAAAGCCA
    ACTAAGACTG TGGTGCCACC AGTGGGTACT GAACTTTCAG CCGGTACTGT ACCCTGTGAG
    CAGCTGCCAC CTTTTCCTGG ACCTTCTCTA ATTCAGCCTT TGTCTACGAT GTCTTCAACA
    ACAGTTACAG AAGCAGGGAC TCGGCTTCAA AAGGATGGCA CAGAAGGTCA TGTCACAGCA
    ACTAGTTCAG GAGCTGGTGT TGTTAAGATG GGGCGATTTC AGGTTTCAGT TACAATGGAT
    GATGCCCAGA AAGAACGTAA AAATAGGTCA GAAGATACAA AGTCGGTTCA TTTTGAATCT
    AGCACATCAG AGTCATCAGT TCTTTCAAGT AGTAGTCCAG AGAGTACCCT GGTAAAGCCA
    GAGCCTAATG GAATTAGTAT TTCTGGCATC TCCTTAGATG TCCCAGACAG CACCCACAAA
    GCACCTACCC CAGAAGCAAA GTCAGATGCA GGACAGCCTA CCAAGGTGGG ACGTTTTCAG
    GTGACAACTA CAGCCAACAA AGTGGGTCGT TTCTCTGTTT CAAGAACTGA GGACAAGGTC
    ACAGAGCTCA AAAAAGAGGG ACCAGTGACC TCTCCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGTGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATTTG
    AATGGGCCAT CTTCAGACCT GGAGGCTGCC TTTCTAAGTA GGGGTACAGA GGATGGCTCA
    GGTAGTCCAC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCGTCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCA TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTACGA CGACTACGAG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACTAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CGGGGAGAAG AAGGAGACCC
    ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC AGCAGCTCAT TGGGCAATAA AAGCCCACAG
    CTTTCAGGCA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TACACCCCCA ACAGACCCTC
    CATCCCGCAG GAAACACCCC CGAGACTGGG CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGGACCAGC AGCACAAATA CCGTTGGAGG AACAGTGAGT
    AGCCAGGCTG CCCAGGCTCA GCCTCCTGCT ATGACTTCCA GTAGGAAGGG CACATTCACA
    GATGACCTGC ACAAGTTAGT AGACAACTGG GCCCGAGATG CCATGAATCT TTCAGGCAGG
    AGAGGCAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT
    CCTGGGCAGC TGTGTGTTCC CATGACCTCA AACCTGGGTG GCTCTACCCC CATCTCTGCA
    GCATCTGCTA CCTCTCTAGG TCACTTCACC AAATCCATGT GTCCCCCACA GCAGTATGGT
    TTTCCACCTG CCCCATTTGG CACTCAGTGG AGTGGAACAG GTGGTCCAGC ACCGCAGCCA
    CTTGGCCAGT TCCAACCTGT AGGAACAGCA TCCTTGCAAA ATTTCAACAT CAGCAATTTG
    CAGAAGTCCA TCAGCAACCC CCCAGGTTCC AATCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu73608
    Clone ID Related Accession (Same CDS sequence) XM_011241320.2
    Accession Version XM_011241320.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7293bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein kinase WNK1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGGGAATAC AGCCAACTGT TCCTTCTCAA CAGGCAGTAC AGTATTCACT TCCACAAGCA
    GCATCTTCCA GTGAAGGTAC TACTGCACAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GACAGTAGCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTCCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAGAC AGGTTTCTCA TCCGTTCCTA TAACAATGGC TGCTGGCATT
    AACCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGGATCA
    CCTGTAGTTC CTAATCAGCT TCCAACTCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTTCTACA GCCAACAACA GTTCAGTCCA TAGGAATACC AGCTAACCTT
    GGACAAGCTG CTGAGGGTCC ACTTCCCTCT GGAGATGTTT TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTTCTATGC CGACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTAAC GCAACAACCC
    CCAACTACAT CCTCCCAGCA AGCAGTTCTG GAGAGTACTC AGGGAGTCTC TCAGGCTGCT
    CCTCCAGAAC AAACTCCAAT AACACAGTCA CAACCTACTC AGCCTGTTCC ATTGGTTACC
    TCTGCAGACA GTGCACATTC AGACGTTGCT TCTGGTATGA GTGATGGCAA TGAGAATGCT
    CCATCATCCA GCGGGAGGCA TGAAGGGAGG ACAACAAAAC GGCATTATAG AAAATCCGTA
    AGAAGTCGCT CTCGGCATGA AAAGACTTCT CGCCCAAAAC TGAGGATTTT AAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTAGAGACTC ATAATCGGAA GATGGTTACA
    TTTAAATTTG ACCTAGATGG TGACAACCCT GAAGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCAATAGA GAGAGAGTCT TTTGTGGCTC AAGTGCGGGA AATTATTGAA
    AAGGCTGATG AAATGTTAAG TGAGGATGTC AGTGTGGAGC CTGAGGGTGA CCAGGGATTG
    GAGAGTCTGC AAGGAAAGGA TGACTATGGC TTCCCAGGTT CTCAGAAATT AGAAGGAGAG
    TTCAAGCAAC CAATTGCTGT ATCTTCCATG CCACAACAAA TAGGTGTTCC TACCAGTTCT
    TTAACGCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT ACCTGAAAGT
    AGGTTACGAG AATCAAAAGT TTTCACCAGT GACATATCAG ATCCAGTTGT GGCCTCTACA
    TCTCAGGCCC CTGGAATGAA CTTATCTCAC TCTGCTTCAT CCCTCAGTCT CCAGCAGGCT
    TTTTCTGAAC TGAAACATGG TCAGATGACT GAAGGACCCA ATACAGCACC TCCAAATTTT
    AATCACATGG CGGGACCAAC ATTTTCTCCC TTTTTGGCTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCGTC AGTATCAGTC CCTATAACAA GCAGTCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC TGAGACTGCA CTGCCCACTG AAAAAGGCAT CGTGGGAGTT
    ACCACTACCA GCACAGGTGT GGTAGCTTCA GGTGGTCTCA CCACAATGTC TGTGTCTGAG
    TCACCAACAT CTTCCAGTGC GGTTTCAAGT TCTACAGTAC CTGCAGTTGT CACTGTGTCC
    ACACCATCCC AGCCAGTTCA GGCTTCCACA TCTGGGAGCA TTGCTTCAAG TACAGGCAGT
    TTTCCTCCTG GAACATTTTC TACAACTACA GCTACTACAA TGGGTAGTGT GGTGGCCCCA
    GATGCTAAGC CTCCAACTGT ATTATTGCAA CAGGTAGCAA GCAATACTGC TGGGGTTGCC
    ATAGTGACAT CAGTCTCTAC CACCACTCCA TTCCCAGGCA TGGCTTCACA GCCATCCCTT
    CCACTTAGTA GCAGCACCTC TGCTCCTACT CTAGCTGAAA CAATGGTGGT TAGTGCACAC
    TCACTAGACA AGGCATCTCA CAGTAGTACA GCAGGATTGG GTTTGTCCTT TTGTGCGCCA
    TCCTCTTCCT CCTCCTCTGG AACAGCTGTG TCTACTTCTG TTTCTCAGCC TGGTATGGTT
    CATCCTTTGG TCATCTCATC AGCTGTAGTT TCTACACCTG GCCTCCCCCA ACCAGTAGTA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAATATCC CACCCTTGGT ACAACCTGTT
    GTTAATGTGC CTGCTGTACA GCAAACACTC ATTCACAGTC AGCCTCAACC AGCTCTGCTT
    CCCAACCAGC CTCACACACA CTGTCCTGAA ATGGATGCTG ATACCCAATC CAAAGCTCCT
    GGGATTGATG ATATAAAGAC TCTGGAGGAA AAGCTGAGAT CTCTCTTTAG TGAACACAGC
    TCATCTGGAA CTCAGCATGC TTCTGTCTCC CTAGAGACAC CTTTAGTAGT AGAGACGACT
    GTCACACCGG GCATCACTAC CACTGCTGTA GCACCAAGTA AACTCATGAC TTCCACTACA
    AGTACTTGCT TACCACCAAC CAGTTTACCA TTAGGAGCAG CTGGCATGCC AGTTATGCCA
    GTAGGCACAC CTGGGCAGGT TTCTACCCCA GGCACACACG CTTCAGCCCC AGTCGGCACT
    GCAACAGGAG TAAAACCTGG AACAACTCCC CCAAAGCCAA CTAAGACTGT GGTGCCACCA
    GTGGGTACTG AACTTTCAGC CGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCTGGA
    CCTTCTCTAA TTCAGTCCCA GCAACCACTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTAC AGTGGCACCT GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACAACAGTTA CAGAAGCAGG GACTCGGCTT CAAAAGGATG GCACAGAAGG TCATGTCACA
    GCAACTAGTT CAGGAGCTGG TGTTGTTAAG ATGGGGCGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAACG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGCACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAG TATTTCTGGC ATCTCCTTAG ATGTCCCAGA CAGCACCCAC
    AAAGCACCTA CCCCAGAAGC AAAGTCAGAT GCAGGACAGC CTACCAAGGT GGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTC CTTTCAGAGA TTCAGAACAA
    ACTGTTATTC CTGCTGTGAT ACCAAAGAAG GAGAAGCCTG AGCTGGCAGA ACCTTCCCAT
    TTGAATGGGC CATCTTCAGA CCTGGAGGCT GCCTTTCTAA GTAGGGGTAC AGAGGATGGC
    TCAGGTAGTC CACACTCTCC CCCTCACCTG TGTTCAAAGA GCCTTCCCGT CCAGAATCTA
    AGTCAAAGCC TTAGTAATTC ATTCAACTCC TCATACATGA GTAGTGACAA TGAGTCCGAC
    ATTGAAGATG AAGACTTAAG GTTAGAACTA CGACGACTAC GAGAAAAACA TCTTAAAGAG
    ATTCAGGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC
    AAGGTTCCCC CTGCTGTCAT TATTCCCCCA GCTGCTCCTC TGTCGGGGAG AAGAAGGAGA
    CCCACTAAAA GCAAAGGCAG CAAGTCTAGT CGCAGCAGCT CATTGGGCAA TAAAAGCCCA
    CAGCTTTCAG GCAACCTGTC TGGTCAGAGT GGAACTTCAG TCTTACACCC CCAACAGACC
    CTCCATCCCG CAGGAAACAC CCCCGAGACT GGGCACAACC AGCTGTTACA GCCTCTTAAG
    CCATCTCCCT CCAGTGACAA CCTCTATTCA GCCTTTACCA GTGATGGTGC CATTTCAGTA
    CCAAGCCTTT CTGCTCCAGG TCAAGGCTGT GCGAAGTTTA ACTGTGCATC TGAGCAGGTG
    ACATTCAAAC CTGGTGGCAG GAGGACCCGA TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG
    AAGATGGTGA AAAAAGTCTG TCCTTGCAAC CAGCTCTGTA TGTGCCCTCC AGCTGAACCG
    AAATCAGGGA CCAGCAGCAC AAATACCGTT GGAGGAACAG TGAGTAGCCA GGCTGCCCAG
    GCTCAGCCTC CTGCTATGAC TTCCAGTAGG AAGGGCACAT TCACAGATGA CCTGCACAAG
    TTAGTAGACA ACTGGGCCCG AGATGCCATG AATCTTTCAG GCAGGAGAGG CAGCAAAGGA
    CACATGAATT ATGAGGGCCC TGGAATGGCA AGGAAGTTCT CTGCTCCTGG GCAGCTGTGT
    GTTCCCATGA CCTCAAACCT GGGTGGCTCT ACCCCCATCT CTGCAGCATC TGCTACCTCT
    CTAGGTCACT TCACCAAATC CATGTGTCCC CCACAGCAGT ATGGTTTTCC ACCTGCCCCA
    TTTGGCACTC AGTGGAGTGG AACAGGTGGT CCAGCACCGC AGCCACTTGG CCAGTTCCAA
    CCTGTAGGAA CAGCATCCTT GCAAAATTTC AACATCAGCA ATTTGCAGAA GTCCATCAGC
    AACCCCCCAG GTTCCAATCT ACGGACCACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011239622.1
    CDS885..8177
    Translation

    Target ORF information:

    RefSeq Version XM_011241320.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011241320.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GTGCCGCCGA GAAGCAAAGC GGCACTCCGG GCTTCCTTAC GCCTCCGGCG 
    CCCGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCTACG
    GTCGCCGACT CGGGAGTTGG CAGGACGGAG GAATATCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACC ACCCCCACGG AGCACCGATT CTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACCGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCGCAACCTA GTGTACCTGC AGTGGTGCCC CAGAGTGCTC CACCCGAACC TCACAGAGAG
    GAAACTTTGA CCGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCACGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGGAGCAAAG AGGAGCCACC GCCGTCCAGA
    AGTGGAAGTG GCAGTGGTGG CGCCAGCGCC AAAGAGGCGC AGGAGGATAG GAGCCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CAGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAGGCTGAA ATGTTAAAGG GTCTTCAACA TCCCAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAG ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GGCTTCAGTT TCTCCACACA
    CGAACTCCAC CAATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACTGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCATACTCAG AATGCCAAAA TGCTGCGCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTAAAAG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATT
    AAATTATGGC TGCGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTTGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTGTCCT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAGCAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGCGTTTCC
    CAGGCAGGAA TCCAGCAGCT CTCTGCTGCT AGCACTGGCA TACCCACTGC TCCTGCCACC
    TCTGCTTCAG TTTCTACACA GGTAGAACCT GAAGAACCTG AGGCAGATCA ACATCAACAG
    CTGCAATATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACCATTGA TAGTGGTCAA
    GGATCATCCG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGTTCC
    CAACATGAGC AGGCACATTC TACTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT GGCTGGGGTT CTATCTTCAC AACCTATCCA ACATCCTCAG
    CAGGGAATAC AGCCAACTGT TCCTTCTCAA CAGGCAGTAC AGTATTCACT TCCACAAGCA
    GCATCTTCCA GTGAAGGTAC TACTGCACAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GACAGTAGCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTCCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAGAC AGGTTTCTCA TCCGTTCCTA TAACAATGGC TGCTGGCATT
    AACCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGGATCA
    CCTGTAGTTC CTAATCAGCT TCCAACTCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTTCTACA GCCAACAACA GTTCAGTCCA TAGGAATACC AGCTAACCTT
    GGACAAGCTG CTGAGGGTCC ACTTCCCTCT GGAGATGTTT TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTTCTATGC CGACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTAAC GCAACAACCC
    CCAACTACAT CCTCCCAGCA AGCAGTTCTG GAGAGTACTC AGGGAGTCTC TCAGGCTGCT
    CCTCCAGAAC AAACTCCAAT AACACAGTCA CAACCTACTC AGCCTGTTCC ATTGGTTACC
    TCTGCAGACA GTGCACATTC AGACGTTGCT TCTGGTATGA GTGATGGCAA TGAGAATGCT
    CCATCATCCA GCGGGAGGCA TGAAGGGAGG ACAACAAAAC GGCATTATAG AAAATCCGTA
    AGAAGTCGCT CTCGGCATGA AAAGACTTCT CGCCCAAAAC TGAGGATTTT AAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTAGAGACTC ATAATCGGAA GATGGTTACA
    TTTAAATTTG ACCTAGATGG TGACAACCCT GAAGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCAATAGA GAGAGAGTCT TTTGTGGCTC AAGTGCGGGA AATTATTGAA
    AAGGCTGATG AAATGTTAAG TGAGGATGTC AGTGTGGAGC CTGAGGGTGA CCAGGGATTG
    GAGAGTCTGC AAGGAAAGGA TGACTATGGC TTCCCAGGTT CTCAGAAATT AGAAGGAGAG
    TTCAAGCAAC CAATTGCTGT ATCTTCCATG CCACAACAAA TAGGTGTTCC TACCAGTTCT
    TTAACGCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT ACCTGAAAGT
    AGGTTACGAG AATCAAAAGT TTTCACCAGT GACATATCAG ATCCAGTTGT GGCCTCTACA
    TCTCAGGCCC CTGGAATGAA CTTATCTCAC TCTGCTTCAT CCCTCAGTCT CCAGCAGGCT
    TTTTCTGAAC TGAAACATGG TCAGATGACT GAAGGACCCA ATACAGCACC TCCAAATTTT
    AATCACATGG CGGGACCAAC ATTTTCTCCC TTTTTGGCTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCGTC AGTATCAGTC CCTATAACAA GCAGTCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC TGAGACTGCA CTGCCCACTG AAAAAGGCAT CGTGGGAGTT
    ACCACTACCA GCACAGGTGT GGTAGCTTCA GGTGGTCTCA CCACAATGTC TGTGTCTGAG
    TCACCAACAT CTTCCAGTGC GGTTTCAAGT TCTACAGTAC CTGCAGTTGT CACTGTGTCC
    ACACCATCCC AGCCAGTTCA GGCTTCCACA TCTGGGAGCA TTGCTTCAAG TACAGGCAGT
    TTTCCTCCTG GAACATTTTC TACAACTACA GCTACTACAA TGGGTAGTGT GGTGGCCCCA
    GATGCTAAGC CTCCAACTGT ATTATTGCAA CAGGTAGCAA GCAATACTGC TGGGGTTGCC
    ATAGTGACAT CAGTCTCTAC CACCACTCCA TTCCCAGGCA TGGCTTCACA GCCATCCCTT
    CCACTTAGTA GCAGCACCTC TGCTCCTACT CTAGCTGAAA CAATGGTGGT TAGTGCACAC
    TCACTAGACA AGGCATCTCA CAGTAGTACA GCAGGATTGG GTTTGTCCTT TTGTGCGCCA
    TCCTCTTCCT CCTCCTCTGG AACAGCTGTG TCTACTTCTG TTTCTCAGCC TGGTATGGTT
    CATCCTTTGG TCATCTCATC AGCTGTAGTT TCTACACCTG GCCTCCCCCA ACCAGTAGTA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAATATCC CACCCTTGGT ACAACCTGTT
    GTTAATGTGC CTGCTGTACA GCAAACACTC ATTCACAGTC AGCCTCAACC AGCTCTGCTT
    CCCAACCAGC CTCACACACA CTGTCCTGAA ATGGATGCTG ATACCCAATC CAAAGCTCCT
    GGGATTGATG ATATAAAGAC TCTGGAGGAA AAGCTGAGAT CTCTCTTTAG TGAACACAGC
    TCATCTGGAA CTCAGCATGC TTCTGTCTCC CTAGAGACAC CTTTAGTAGT AGAGACGACT
    GTCACACCGG GCATCACTAC CACTGCTGTA GCACCAAGTA AACTCATGAC TTCCACTACA
    AGTACTTGCT TACCACCAAC CAGTTTACCA TTAGGAGCAG CTGGCATGCC AGTTATGCCA
    GTAGGCACAC CTGGGCAGGT TTCTACCCCA GGCACACACG CTTCAGCCCC AGTCGGCACT
    GCAACAGGAG TAAAACCTGG AACAACTCCC CCAAAGCCAA CTAAGACTGT GGTGCCACCA
    GTGGGTACTG AACTTTCAGC CGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCTGGA
    CCTTCTCTAA TTCAGTCCCA GCAACCACTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTAC AGTGGCACCT GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACAACAGTTA CAGAAGCAGG GACTCGGCTT CAAAAGGATG GCACAGAAGG TCATGTCACA
    GCAACTAGTT CAGGAGCTGG TGTTGTTAAG ATGGGGCGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAACG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGCACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAG TATTTCTGGC ATCTCCTTAG ATGTCCCAGA CAGCACCCAC
    AAAGCACCTA CCCCAGAAGC AAAGTCAGAT GCAGGACAGC CTACCAAGGT GGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTC CTTTCAGAGA TTCAGAACAA
    ACTGTTATTC CTGCTGTGAT ACCAAAGAAG GAGAAGCCTG AGCTGGCAGA ACCTTCCCAT
    TTGAATGGGC CATCTTCAGA CCTGGAGGCT GCCTTTCTAA GTAGGGGTAC AGAGGATGGC
    TCAGGTAGTC CACACTCTCC CCCTCACCTG TGTTCAAAGA GCCTTCCCGT CCAGAATCTA
    AGTCAAAGCC TTAGTAATTC ATTCAACTCC TCATACATGA GTAGTGACAA TGAGTCCGAC
    ATTGAAGATG AAGACTTAAG GTTAGAACTA CGACGACTAC GAGAAAAACA TCTTAAAGAG
    ATTCAGGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC TAAACTGGGC
    AAGGTTCCCC CTGCTGTCAT TATTCCCCCA GCTGCTCCTC TGTCGGGGAG AAGAAGGAGA
    CCCACTAAAA GCAAAGGCAG CAAGTCTAGT CGCAGCAGCT CATTGGGCAA TAAAAGCCCA
    CAGCTTTCAG GCAACCTGTC TGGTCAGAGT GGAACTTCAG TCTTACACCC CCAACAGACC
    CTCCATCCCG CAGGAAACAC CCCCGAGACT GGGCACAACC AGCTGTTACA GCCTCTTAAG
    CCATCTCCCT CCAGTGACAA CCTCTATTCA GCCTTTACCA GTGATGGTGC CATTTCAGTA
    CCAAGCCTTT CTGCTCCAGG TCAAGGCTGT GCGAAGTTTA ACTGTGCATC TGAGCAGGTG
    ACATTCAAAC CTGGTGGCAG GAGGACCCGA TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG
    AAGATGGTGA AAAAAGTCTG TCCTTGCAAC CAGCTCTGTA TGTGCCCTCC AGCTGAACCG
    AAATCAGGGA CCAGCAGCAC AAATACCGTT GGAGGAACAG TGAGTAGCCA GGCTGCCCAG
    GCTCAGCCTC CTGCTATGAC TTCCAGTAGG AAGGGCACAT TCACAGATGA CCTGCACAAG
    TTAGTAGACA ACTGGGCCCG AGATGCCATG AATCTTTCAG GCAGGAGAGG CAGCAAAGGA
    CACATGAATT ATGAGGGCCC TGGAATGGCA AGGAAGTTCT CTGCTCCTGG GCAGCTGTGT
    GTTCCCATGA CCTCAAACCT GGGTGGCTCT ACCCCCATCT CTGCAGCATC TGCTACCTCT
    CTAGGTCACT TCACCAAATC CATGTGTCCC CCACAGCAGT ATGGTTTTCC ACCTGCCCCA
    TTTGGCACTC AGTGGAGTGG AACAGGTGGT CCAGCACCGC AGCCACTTGG CCAGTTCCAA
    CCTGTAGGAA CAGCATCCTT GCAAAATTTC AACATCAGCA ATTTGCAGAA GTCCATCAGC
    AACCCCCCAG GTTCCAATCT ACGGACCACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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