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Tufm cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Tufm
Entrez Gene ID 233870
Full Name Tu translation elongation factor, mitochondrial
Synonyms 2300002G02Rik,C76308,C76389,EF-TuMT,EFTU
General protein information
Preferred Names

Tu translation elongation factor, mitochondrial

Names

elongation factor Tu, mitochondrial
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

7

7|7 F3

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006507719.4 XP_006507782.1 elongation factor Tu, mitochondrial isoform X1
NM_172745.3 NP_766333.1 elongation factor Tu, mitochondrial isoform 1
XM_006507719.3 XP_006507782.1 elongation factor Tu, mitochondrial isoform X1
NM_001163713.1 NP_001157185.1 elongation factor Tu, mitochondrial isoform 2

Pathways from BioSystems


Homologies


TUFM XP_001103519.1
Homo sapiens (human) TUFM NP_003312.3
Saccharomyces cerevisiae (baker's yeast) TUF1 NP_014830.1
Kluyveromyces lactis KLLA0A04587g XP_451201.1
Kluyveromyces lactis KLLA0C05214g XP_452433.1
Eremothecium gossypii AGOS_AAR143W NP_982685.1
Magnaporthe oryzae (rice blast fungus) MGG_08162 XP_003715132.1
Canis lupus familiaris (dog) TUFM XP_536924.3
Bos taurus (cattle) TUFM NP_776632.1
Rattus norvegicus (Norway rat) Tufm NP_001099765.1
Danio rerio (zebrafish) tufm NP_001013523.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP006996 XP_308774.4
Neurospora crassa NCU03737 XP_961513.1
Pan troglodytes (chimpanzee) TUFM XP_510904.2
Drosophila melanogaster (fruit fly) EfTuM NP_001163144.1
Schizosaccharomyces pombe (fission yeast) tuf1 NP_595746.1
Arabidopsis thaliana (thale cress) AT4G02930 NP_192202.1
Oryza sativa (rice) Os03g0851100 NP_001051912.1
Mus musculus (house mouse) Tufm NP_766333.1
Caenorhabditis elegans (roundworm) tufm-1 NP_497623.1

Gene Ontology


Bibliography

Related articles in PubMed

Fancd2 in vivo interaction network reveals a non-canonical role in mitochondrial function.
Zhang T, Du W, Wilson AF, Namekawa SH, Andreassen PR, Meetei AR, Pang Q
Scientific reports745626(2017 Apr)

The mitochondrial proteins NLRX1 and TUFM form a complex that regulates type I interferon and autophagy.
Lei Y, Wen H, Yu Y, Taxman DJ, Zhang L, Widman DG, Swanson KV, Wen KW, Damania B, Moore CB, Gigu?re PM, Siderovski DP, Hiscott J, Razani B, Semenkovich CF, Chen X, Ting JP
Immunity36(6)933-46(2012 Jun)

Proteolipid protein is required for transport of sirtuin 2 into CNS myelin.
Werner HB, Kuhlmann K, Shen S, Uecker M, Schardt A, Dimova K, Orfaniotou F, Dhaunchak A, Brinkmann BG, M?bius W, Guarente L, Casaccia-Bonnefil P, Jahn O, Nave KA
The Journal of neuroscience : the official journal of the Society for Neuroscience27(29)7717-30(2007 Jul)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

Proteomic analysis of the mouse liver mitochondrial inner membrane.
Da Cruz S, Xenarios I, Langridge J, Vilbois F, Parone PA, Martinou JC
The Journal of biological chemistry278(42)41566-71(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tufm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tufm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu13249 NM_172745.3
Latest version!		 Mus musculus Tu translation elongation factor, mitochondrial (Tufm), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu13180 NM_001163713.1
Latest version!		 Mus musculus Tu translation elongation factor, mitochondrial (Tufm), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $321.30
$459.00
OMu39553 XM_006507719.4
Latest version!		 Mus musculus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OMu39553 XM_006507719.3 Mus musculus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu13249
Clone ID Related Accession (Same CDS sequence) NM_172745.3
Accession Version NM_172745.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-18
Organism Mus musculus(house mouse)
Product elongation factor Tu, mitochondrial isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY246546.1, AK152858.1 and BC060959.1. On Jul 30, 2009 this sequence version replaced NM_172745.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC100596.1, AK152858.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGCAG CCACTCTATT GCGAGCGACG CCCCGCTTCA GCGGTCTCTG CGCCAGCCCG 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCTC CCGCATCACC CTTCTTGTGT 
CGCGGCCTAG CTGTGGAGGC CAAGAAGACT TATGTACGCG ACAAGCCCCA TGTGAATGTG 
GGTACCATCG GACATGTGGA CCATGGCAAG ACCACCCTGA CTGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGCATCAC CATCAATGCA GCCCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG TCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACTGCCCCCC TGGATGGCTG TATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAACAG ATTGGGGTAG AACATGTTGT GGTGTACGTG 
AACAAGGCAG ACGCTGTCCA GGACTCAGAG ATGGTGGAGT TAGTCGAGCT GGAGATCCGG 
GAGCTGCTCA CCGAGTTTGG CTATAAAGGA GAGGAAACTC CAGTCATCGT AGGCTCGGCT 
CTCTGTGCCC TTGAGCAACG TGACCCTGAG CTAGGCGTGA AGTCAGTGCA GAAGCTCCTG 
GATGCTGTGG ACACCTACAT CCCAGTGCCC ACCCGGGACC TGGACAAGCC CTTCCTGCTC 
CCTGTAGAGT CAGTCTACTC TATTCCTGGC CGGGGCACAG TGGTGACAGG TACATTAGAG 
CGTGGCATTT TGAAGAAAGG AGATGAGTGT GAGTTGCTGG GACATAACAA GAACATCCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG AGAGGGCTGA GGCAGGGGAT 
AACCTGGGTG CTCTGGTCCG AGGCTTAAAG CGCGAAGATT TGAGGCGTGG CTTGGTCATG 
GTCAAGCCAG GCTCCATCCA ACCCCACCAG AAGGTGGAGG CCCAGGTTTA TATCCTCAGC 
AAGGAGGAAG GTGGCCGCCA CAAACCCTTT GTATCTCATT TCATGCCCGT CATGTTCTCC 
CTGACCTGGG ACATGGCCTG TCGAGTCATC TTGCCTCCAG GGAAGGAACT TGCCATGCCT 
GGAGAGGACT TGAAGCTTAG TCTAATCTTG CGGCAGCCCA TGATCTTAGA GAAAGGCCAA 
CGTTTCACCT TGAGGGATGG CAACAAGACC ATTGGCACTG GCCTTGTTAC TGATGTACCA 
GCCATGACTG AGGAGGACAA GAACATCAAG TGGAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_766333.1
CDS94..1452
Translation

Target ORF information:

RefSeq Version NM_172745.3
Organism Mus musculus(house mouse)
Definition Mus musculus Tu translation elongation factor, mitochondrial (Tufm), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_172745.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGCAG CCACTCTATT GCGAGCGACG CCCCGCTTCA GCGGTCTCTG CGCCAGCCCG 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCTC CCGCATCACC CTTCTTGTGT 
CGCGGCCTAG CTGTGGAGGC CAAGAAGACT TATGTACGCG ACAAGCCCCA TGTGAATGTG 
GGTACCATCG GACATGTGGA CCATGGCAAG ACCACCCTGA CTGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGCATCAC CATCAATGCA GCCCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG TCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACTGCCCCCC TGGATGGCTG TATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAACAG ATTGGGGTAG AACATGTTGT GGTGTACGTG 
AACAAGGCAG ACGCTGTCCA GGACTCAGAG ATGGTGGAGT TAGTCGAGCT GGAGATCCGG 
GAGCTGCTCA CCGAGTTTGG CTATAAAGGA GAGGAAACTC CAGTCATCGT AGGCTCGGCT 
CTCTGTGCCC TTGAGCAACG TGACCCTGAG CTAGGCGTGA AGTCAGTGCA GAAGCTCCTG 
GATGCTGTGG ACACCTACAT CCCAGTGCCC ACCCGGGACC TGGACAAGCC CTTCCTGCTC 
CCTGTAGAGT CAGTCTACTC TATTCCTGGC CGGGGCACAG TGGTGACAGG TACATTAGAG 
CGTGGCATTT TGAAGAAAGG AGATGAGTGT GAGTTGCTGG GACATAACAA GAACATCCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG AGAGGGCTGA GGCAGGGGAT 
AACCTGGGTG CTCTGGTCCG AGGCTTAAAG CGCGAAGATT TGAGGCGTGG CTTGGTCATG 
GTCAAGCCAG GCTCCATCCA ACCCCACCAG AAGGTGGAGG CCCAGGTTTA TATCCTCAGC 
AAGGAGGAAG GTGGCCGCCA CAAACCCTTT GTATCTCATT TCATGCCCGT CATGTTCTCC 
CTGACCTGGG ACATGGCCTG TCGAGTCATC TTGCCTCCAG GGAAGGAACT TGCCATGCCT 
GGAGAGGACT TGAAGCTTAG TCTAATCTTG CGGCAGCCCA TGATCTTAGA GAAAGGCCAA 
CGTTTCACCT TGAGGGATGG CAACAAGACC ATTGGCACTG GCCTTGTTAC TGATGTACCA 
GCCATGACTG AGGAGGACAA GAACATCAAG TGGAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu39553
Clone ID Related Accession (Same CDS sequence) XM_006507719.4 , XM_006507719.3
Accession Version XM_006507719.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product elongation factor Tu, mitochondrial isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507719.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCAG CCACTCTATT GCGAGCGACG CCCCGCTTCA GCGGTCTCTG CGCCAGCCCG 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCTC CCGCATCACC CTTCTTGTGT 
CGCGGCCTAG CTGTGGAGGC CAAGAAGACT TATGTACGCG ACAAGCCCCA TGTGAATGTG 
GGTACCATCG GACATGTGGA CCATGGCAAG ACCACCCTGA CTGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGCATCAC CATCAATGCA GCCCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG TCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACTGCCCCCC TGGATGGCTG TATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAACAG ATTGGGGTAG AACATGTTGT GGTGTACGTG 
AACAAGGCAG ACGCTGTCCA GGACTCAGAG ATGGTGGAGT TAGTCGAGCT GGAGATCCGG 
GAGCTGCTCA CCGAGTTTGG CTATAAAGGA GAGGAAACTC CAGTCATCGT AGGCTCGGCT 
CTCTGTGCCC TTGAGCAACG TGACCCTGAG CTAGGCGTGA AGTCAGTGCA GAAGCTCCTG 
GATGCTGTGG ACACCTACAT CCCAGTGCCC ACCCGGGACC TGGACAAGCC CTTCCTGCTC 
CCTGTAGAGT CAGTCTACTC TATTCCTGGC CGGGGCACAG TGGTGACAGG CATTGAGATG 
TTCCACAAGA GCCTGGAGAG GGCTGAGGCA GGGGATAACC TGGGTGCTCT GGTCCGAGGC 
TTAAAGCGCG AAGATTTGAG GCGTGGCTTG GTCATGGTCA AGCCAGGCTC CATCCAACCC 
CACCAGAAGG TGGAGGCCCA GGTTTATATC CTCAGCAAGG AGGAAGGTGG CCGCCACAAA 
CCCTTTGTAT CTCATTTCAT GCCCGTCATG TTCTCCCTGA CCTGGGACAT GGCCTGTCGA 
GTCATCTTGC CTCCAGGGAA GGAACTTGCC ATGCCTGGAG AGGACTTGAA GCTTAGTCTA 
ATCTTGCGGC AGCCCATGAT CTTAGAGAAA GGCCAACGTT TCACCTTGAG GGATGGCAAC 
AAGACCATTG GCACTGGCCT TGTTACTGAT GTACCAGCCA TGACTGAGGA GGACAAGAAC 
ATCAAGTGGA GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507782.1
CDS95..1369
Translation

Target ORF information:

RefSeq Version XM_006507719.3
Organism Mus musculus(house mouse)
Definition Mus musculus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006507719.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCAG CCACTCTATT GCGAGCGACG CCCCGCTTCA GCGGTCTCTG CGCCAGCCCG 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCTC CCGCATCACC CTTCTTGTGT 
CGCGGCCTAG CTGTGGAGGC CAAGAAGACT TATGTACGCG ACAAGCCCCA TGTGAATGTG 
GGTACCATCG GACATGTGGA CCATGGCAAG ACCACCCTGA CTGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGCATCAC CATCAATGCA GCCCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG TCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACTGCCCCCC TGGATGGCTG TATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAACAG ATTGGGGTAG AACATGTTGT GGTGTACGTG 
AACAAGGCAG ACGCTGTCCA GGACTCAGAG ATGGTGGAGT TAGTCGAGCT GGAGATCCGG 
GAGCTGCTCA CCGAGTTTGG CTATAAAGGA GAGGAAACTC CAGTCATCGT AGGCTCGGCT 
CTCTGTGCCC TTGAGCAACG TGACCCTGAG CTAGGCGTGA AGTCAGTGCA GAAGCTCCTG 
GATGCTGTGG ACACCTACAT CCCAGTGCCC ACCCGGGACC TGGACAAGCC CTTCCTGCTC 
CCTGTAGAGT CAGTCTACTC TATTCCTGGC CGGGGCACAG TGGTGACAGG CATTGAGATG 
TTCCACAAGA GCCTGGAGAG GGCTGAGGCA GGGGATAACC TGGGTGCTCT GGTCCGAGGC 
TTAAAGCGCG AAGATTTGAG GCGTGGCTTG GTCATGGTCA AGCCAGGCTC CATCCAACCC 
CACCAGAAGG TGGAGGCCCA GGTTTATATC CTCAGCAAGG AGGAAGGTGG CCGCCACAAA 
CCCTTTGTAT CTCATTTCAT GCCCGTCATG TTCTCCCTGA CCTGGGACAT GGCCTGTCGA 
GTCATCTTGC CTCCAGGGAA GGAACTTGCC ATGCCTGGAG AGGACTTGAA GCTTAGTCTA 
ATCTTGCGGC AGCCCATGAT CTTAGAGAAA GGCCAACGTT TCACCTTGAG GGATGGCAAC 
AAGACCATTG GCACTGGCCT TGTTACTGAT GTACCAGCCA TGACTGAGGA GGACAAGAAC 
ATCAAGTGGA GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu13180
Clone ID Related Accession (Same CDS sequence) NM_001163713.1
Accession Version NM_001163713.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1308bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-27
Organism Mus musculus(house mouse)
Product elongation factor Tu, mitochondrial isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY246546.1, AK152858.1, BU699735.1 and BC060959.1. Transcript Variant: This variant (2) includes an alternate segment that results in a frameshift compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC060959.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCAG CCACTCTATT GCGAGCGACG CCCCGCTTCA GCGGTCTCTG CGCCAGCCCG 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCTC CCGCATCACC CTTCTTGTGT 
CGCGGCCTAG CTGTGGAGGC CAAGAAGACT TATGTACGCG ACAAGCCCCA TGTGAATGTG 
GGTACCATCG GACATGTGGA CCATGGCAAG ACCACCCTGA CTGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGCATCAC CATCAATGCA GCCCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG TCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACTGCCCCCC TGGATGGCTG TATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAACAG ATTGGGGTAG AACATGTTGT GGTGTACGTG 
AACAAGGCAG ACGCTGTCCA GGACTCAGAG ATGGTGGAGT TAGTCGAGCT GGAGATCCGG 
GAGCTGCTCA CCGAGTTTGG CTATAAAGGA GAGGAAACTC CAGTCATCGT AGGCTCGGCT 
CTCTGTGCCC TTGAGCAACG TGACCCTGAG CTAGGCGTGA AGTCAGTGCA GAAGCTCCTG 
GATGCTGTGG ACACCTACAT CCCAGTGCCC ACCCGGGACC TGGACAAGCC CTTCCTGCTC 
CCTGTAGAGT CAGTCTACTC TATTCCTGGC CGGGGCACAG TGGTGACAGG TACATTAGAG 
CGTGGCATTT TGAAGAAAGG AGATGAGTGT GAGTTGCTGG GACATAACAA GAACATCCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG AGAGGGCTGA GGCAGGGGAT 
AACCTGGGTG CTCTGGTCCG AGGCTTAAAG CGCGAAGATT TGAGGCGTGG CTTGGTCATG 
GTCAAGCCAG GCTCCATCCA ACCCCACCAG AAGGTGGAGG CCCAGGTGAG GGCTCCAGTG 
CTGAGTGGTT TCCCTTGCTT AGAAGCAGGA CTTGTAGCTA AGCCATTCCA TCCTTACTGC 
TTCTCTCCTC TAGGTTTATA TCCTCAGCAA GGAGGAAGGT GGCCGCCACA AACCCTTTGT 
ATCTCATTTC ATGCCCGTCA TGTTCTCCCT GACCTGGGAC ATGGCCTGTC GAGTCATCTT 
GCCTCCAGGG AAGGAACTTG CCATGCCTGG AGAGGACTTG AAGCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157185.1
CDS94..1401
Translation

Target ORF information:

RefSeq Version NM_001163713.1
Organism Mus musculus(house mouse)
Definition Mus musculus Tu translation elongation factor, mitochondrial (Tufm), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001163713.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCAG CCACTCTATT GCGAGCGACG CCCCGCTTCA GCGGTCTCTG CGCCAGCCCG 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCTC CCGCATCACC CTTCTTGTGT 
CGCGGCCTAG CTGTGGAGGC CAAGAAGACT TATGTACGCG ACAAGCCCCA TGTGAATGTG 
GGTACCATCG GACATGTGGA CCATGGCAAG ACCACCCTGA CTGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGCATCAC CATCAATGCA GCCCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG TCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACTGCCCCCC TGGATGGCTG TATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAACAG ATTGGGGTAG AACATGTTGT GGTGTACGTG 
AACAAGGCAG ACGCTGTCCA GGACTCAGAG ATGGTGGAGT TAGTCGAGCT GGAGATCCGG 
GAGCTGCTCA CCGAGTTTGG CTATAAAGGA GAGGAAACTC CAGTCATCGT AGGCTCGGCT 
CTCTGTGCCC TTGAGCAACG TGACCCTGAG CTAGGCGTGA AGTCAGTGCA GAAGCTCCTG 
GATGCTGTGG ACACCTACAT CCCAGTGCCC ACCCGGGACC TGGACAAGCC CTTCCTGCTC 
CCTGTAGAGT CAGTCTACTC TATTCCTGGC CGGGGCACAG TGGTGACAGG TACATTAGAG 
CGTGGCATTT TGAAGAAAGG AGATGAGTGT GAGTTGCTGG GACATAACAA GAACATCCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG AGAGGGCTGA GGCAGGGGAT 
AACCTGGGTG CTCTGGTCCG AGGCTTAAAG CGCGAAGATT TGAGGCGTGG CTTGGTCATG 
GTCAAGCCAG GCTCCATCCA ACCCCACCAG AAGGTGGAGG CCCAGGTGAG GGCTCCAGTG 
CTGAGTGGTT TCCCTTGCTT AGAAGCAGGA CTTGTAGCTA AGCCATTCCA TCCTTACTGC 
TTCTCTCCTC TAGGTTTATA TCCTCAGCAA GGAGGAAGGT GGCCGCCACA AACCCTTTGT 
ATCTCATTTC ATGCCCGTCA TGTTCTCCCT GACCTGGGAC ATGGCCTGTC GAGTCATCTT 
GCCTCCAGGG AAGGAACTTG CCATGCCTGG AGAGGACTTG AAGCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu39553
Clone ID Related Accession (Same CDS sequence) XM_006507719.4 , XM_006507719.3
Accession Version XM_006507719.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product elongation factor Tu, mitochondrial isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507719.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCAG CCACTCTATT GCGAGCGACG CCCCGCTTCA GCGGTCTCTG CGCCAGCCCG 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCTC CCGCATCACC CTTCTTGTGT 
CGCGGCCTAG CTGTGGAGGC CAAGAAGACT TATGTACGCG ACAAGCCCCA TGTGAATGTG 
GGTACCATCG GACATGTGGA CCATGGCAAG ACCACCCTGA CTGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGCATCAC CATCAATGCA GCCCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG TCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACTGCCCCCC TGGATGGCTG TATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAACAG ATTGGGGTAG AACATGTTGT GGTGTACGTG 
AACAAGGCAG ACGCTGTCCA GGACTCAGAG ATGGTGGAGT TAGTCGAGCT GGAGATCCGG 
GAGCTGCTCA CCGAGTTTGG CTATAAAGGA GAGGAAACTC CAGTCATCGT AGGCTCGGCT 
CTCTGTGCCC TTGAGCAACG TGACCCTGAG CTAGGCGTGA AGTCAGTGCA GAAGCTCCTG 
GATGCTGTGG ACACCTACAT CCCAGTGCCC ACCCGGGACC TGGACAAGCC CTTCCTGCTC 
CCTGTAGAGT CAGTCTACTC TATTCCTGGC CGGGGCACAG TGGTGACAGG CATTGAGATG 
TTCCACAAGA GCCTGGAGAG GGCTGAGGCA GGGGATAACC TGGGTGCTCT GGTCCGAGGC 
TTAAAGCGCG AAGATTTGAG GCGTGGCTTG GTCATGGTCA AGCCAGGCTC CATCCAACCC 
CACCAGAAGG TGGAGGCCCA GGTTTATATC CTCAGCAAGG AGGAAGGTGG CCGCCACAAA 
CCCTTTGTAT CTCATTTCAT GCCCGTCATG TTCTCCCTGA CCTGGGACAT GGCCTGTCGA 
GTCATCTTGC CTCCAGGGAA GGAACTTGCC ATGCCTGGAG AGGACTTGAA GCTTAGTCTA 
ATCTTGCGGC AGCCCATGAT CTTAGAGAAA GGCCAACGTT TCACCTTGAG GGATGGCAAC 
AAGACCATTG GCACTGGCCT TGTTACTGAT GTACCAGCCA TGACTGAGGA GGACAAGAAC 
ATCAAGTGGA GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507782.1
CDS178..1452
Translation

Target ORF information:

RefSeq Version XM_006507719.4
Organism Mus musculus(house mouse)
Definition Mus musculus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006507719.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCAG CCACTCTATT GCGAGCGACG CCCCGCTTCA GCGGTCTCTG CGCCAGCCCG 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCTC CCGCATCACC CTTCTTGTGT 
CGCGGCCTAG CTGTGGAGGC CAAGAAGACT TATGTACGCG ACAAGCCCCA TGTGAATGTG 
GGTACCATCG GACATGTGGA CCATGGCAAG ACCACCCTGA CTGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGCATCAC CATCAATGCA GCCCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG TCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACTGCCCCCC TGGATGGCTG TATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAACAG ATTGGGGTAG AACATGTTGT GGTGTACGTG 
AACAAGGCAG ACGCTGTCCA GGACTCAGAG ATGGTGGAGT TAGTCGAGCT GGAGATCCGG 
GAGCTGCTCA CCGAGTTTGG CTATAAAGGA GAGGAAACTC CAGTCATCGT AGGCTCGGCT 
CTCTGTGCCC TTGAGCAACG TGACCCTGAG CTAGGCGTGA AGTCAGTGCA GAAGCTCCTG 
GATGCTGTGG ACACCTACAT CCCAGTGCCC ACCCGGGACC TGGACAAGCC CTTCCTGCTC 
CCTGTAGAGT CAGTCTACTC TATTCCTGGC CGGGGCACAG TGGTGACAGG CATTGAGATG 
TTCCACAAGA GCCTGGAGAG GGCTGAGGCA GGGGATAACC TGGGTGCTCT GGTCCGAGGC 
TTAAAGCGCG AAGATTTGAG GCGTGGCTTG GTCATGGTCA AGCCAGGCTC CATCCAACCC 
CACCAGAAGG TGGAGGCCCA GGTTTATATC CTCAGCAAGG AGGAAGGTGG CCGCCACAAA 
CCCTTTGTAT CTCATTTCAT GCCCGTCATG TTCTCCCTGA CCTGGGACAT GGCCTGTCGA 
GTCATCTTGC CTCCAGGGAA GGAACTTGCC ATGCCTGGAG AGGACTTGAA GCTTAGTCTA 
ATCTTGCGGC AGCCCATGAT CTTAGAGAAA GGCCAACGTT TCACCTTGAG GGATGGCAAC 
AAGACCATTG GCACTGGCCT TGTTACTGAT GTACCAGCCA TGACTGAGGA GGACAAGAAC 
ATCAAGTGGA GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Fancd2 in vivo interaction network reveals a non-canonical role in mitochondrial function.
Scientific reports745626(2017 Apr)
Zhang T,Du W,Wilson AF,Namekawa SH,Andreassen PR,Meetei AR,Pang Q


book

The mitochondrial proteins NLRX1 and TUFM form a complex that regulates type I interferon and autophagy.
Immunity36(6)933-46(2012 Jun)
Lei Y,Wen H,Yu Y,Taxman DJ,Zhang L,Widman DG,Swanson KV,Wen KW,Damania B,Moore CB,Gigu?re PM,Siderovski DP,Hiscott J,Razani B,Semenkovich CF,Chen X,Ting JP


book

Proteolipid protein is required for transport of sirtuin 2 into CNS myelin.
The Journal of neuroscience : the official journal of the Society for Neuroscience27(29)7717-30(2007 Jul)
Werner HB,Kuhlmann K,Shen S,Uecker M,Schardt A,Dimova K,Orfaniotou F,Dhaunchak A,Brinkmann BG,M?bius W,Guarente L,Casaccia-Bonnefil P,Jahn O,Nave KA


book

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M


book

Proteomic analysis of the mouse liver mitochondrial inner membrane.
The Journal of biological chemistry278(42)41566-71(2003 Oct)
Da Cruz S,Xenarios I,Langridge J,Vilbois F,Parone PA,Martinou JC