ORF cDNA Clones and Custom Clones » Species Summary » Mus musculus » Eml6 cDNA ORF clone

Eml6 cDNA ORF clone, Mus musculus(house mouse)

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Summary

Gene Symbol	Eml6
Entrez Gene ID	237711
Full Name	echinoderm microtubule associated protein like 6
Synonyms	2900083P10Rik,C230094A16Rik,EMAP-6
General protein information	Preferred Names echinoderm microtubule associated protein like 6 Names echinoderm microtubule-associated protein-like 6
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 A3.3

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314516.1	XP_017170005.1	echinoderm microtubule-associated protein-like 6 isoform X5
XM_006514695.3	XP_006514758.1	echinoderm microtubule-associated protein-like 6 isoform X7
XM_017314514.2	XP_017170003.1	echinoderm microtubule-associated protein-like 6 isoform X3
XM_017314516.2	XP_017170005.1	echinoderm microtubule-associated protein-like 6 isoform X5
XM_017314517.1	XP_017170006.1	echinoderm microtubule-associated protein-like 6 isoform X6
XM_017314513.1	XP_017170002.1	echinoderm microtubule-associated protein-like 6 isoform X2
XM_017314514.1	XP_017170003.1	echinoderm microtubule-associated protein-like 6 isoform X3
NM_146016.2	NP_666128.2	echinoderm microtubule-associated protein-like 6
XM_017314515.1	XP_017170004.1	echinoderm microtubule-associated protein-like 6 isoform X4
XM_017314515.2	XP_017170004.1	echinoderm microtubule-associated protein-like 6 isoform X4
XM_006514695.4	XP_006514758.1	echinoderm microtubule-associated protein-like 6 isoform X7
XM_006514694.3	XP_006514757.1	echinoderm microtubule-associated protein-like 6 isoform X1
XM_006514694.4	XP_006514757.1	echinoderm microtubule-associated protein-like 6 isoform X1
XM_017314513.2	XP_017170002.1	echinoderm microtubule-associated protein-like 6 isoform X2
XM_017314517.2	XP_017170006.1	echinoderm microtubule-associated protein-like 6 isoform X6

Homologies

Pan troglodytes (chimpanzee)	EML6	XP_003309102.1
Mus musculus (house mouse)	Eml6	NP_666128.2
Rattus norvegicus (Norway rat)	RGD1561672	XP_006251672.1
Xenopus tropicalis (tropical clawed frog)	eml6	XP_004914799.1
	EML6	XP_001112056.2
Gallus gallus (chicken)	EML6	XP_003640942.2
Homo sapiens (human)	EML6	NP_001034842.2
Canis lupus familiaris (dog)	EML6	XP_005626297.1
Bos taurus (cattle)	EML6	NP_001179386.1
Danio rerio (zebrafish)	eml6	XP_001919110.4

Bibliography

Related articles in PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

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The following Eml6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eml6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu45207	XM_006514695.4 Latest version!	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X8, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	19-21	$643.30 $919.00
OMu45206	XM_006514694.4 Latest version!	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	24-26	$951.30 $1359.00
OMu77887	XM_017314513.2 Latest version!	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	24-26	$951.30 $1359.00
OMu77888	XM_017314514.2 Latest version!	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	24-26	$951.30 $1359.00
OMu77889	XM_017314515.2 Latest version!	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X4, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	24-26	$951.30 $1359.00
OMu77890	XM_017314516.2 Latest version!	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X5, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	24-26	$951.30 $1359.00
OMu77891	XM_017314517.2 Latest version!	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X6, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	24-26	$951.30 $1359.00
OMu20880	NM_146016.2 Latest version!	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu77890	XM_017314516.1	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X5, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	24-26	$951.30 $1359.00
OMu77888	XM_017314514.1	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	24-26	$951.30 $1359.00
OMu77889	XM_017314515.1	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X4, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	24-26	$951.30 $1359.00
OMu45207	XM_006514695.3	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X8, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	20	$643.30 $919.00
OMu45206	XM_006514694.3	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	25	$951.30 $1359.00
OMu77887	XM_017314513.1	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	24-26	$951.30 $1359.00
OMu77891	XM_017314517.1	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X6, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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Reference Sequences (Refseq)

Eml6 ( XM_006514695.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514758.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu45207
Clone ID Related Accession (Same CDS sequence)	XM_006514695.3 , XM_006514695.4
Accession Version	XM_006514695.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514695.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACT ACAAGAGATT TGGAAGAACA GATGTATCTG ACGGAAAATG TCTCGTGTCG 
GTTGGTTTAG ACGATTTTCA CAGTGTTGTG TTTTGGGACT GGAAAAAGGG AGAAAAGATA 
GCCACAACCA GGGGACACAA AGACAAAATA TTTGTGGTAA AGTGTAATCC ACAGCATGCT 
GACAAGTTGG TGACAGTTGG GATTAAGCAT ATCAAGTTCT GGCAACAAGC AGGTGGGGGC 
TTCACCTCTA AAAGAGGAAG TTTTGGAAGT GCTGGAAAGT TGGAAACAAT GATGTGTGTT 
TCTTATGGGC GAATGGAAGA TCTGGTGTTC TCAGGTGCAG CCACTGGAGA TATTTTTATC 
TGGAAGGACG TTCTGCTACT GAAGACAGTG AAGGCTCATG ATGGGCCTGT GTTTGCCATG 
TATGCATTAG ACAAGGGCTT TGTAACAGGT GGAAAGGATG GAATTGTGGA ACTCTGGGAT 
GATATGTTTG AAAGATGCTT GAAGACTTAT GCCATCAAAA GGACTGCATT GTCAACCAGC 
TCAAAAGGAC TGCTTTTGGA GGATAACCCT TCAATTCGTG CAATTACCCT AGGGCATGGA 
CACATTCTCG TGGGGACAAA AAATGGAGAG ATTCTAGAAA TTGATAAGAG TGGCCCGATG 
ACCCTGCTTG TTCAGGGACA CATGGAAGGG GAAGTGTGGG GGCTGGCAGC ACACCCGCTC 
CTGCCCATCT GCGCGACAGT AAGTGATGAC AAAACACTTC GGATCTGGGA ATTATCCTCC 
CAGCACCGCA TGCTGGCTGT ACGGAAACTT AAAAAAGGAG GAAGATGTTG TGCTTTCTCC 
CCTGATGGGA AAGCCCTAGC AGTTGGATTG AATGATGGAA GTTTCTTGGT GGTGAATGCT 
GACACAGTGG AAGACATGCT CTCCTTCCAT CACAGAAAAG AAATGATCTC TGATATAAAA 
TTTTCAAAAG ATACAGGAAA ATACCTTGCT GTGGCATCTC ATGATAACTT CGTGGATATC 
TACAACGTGC TTACAAGCAA GAGGGTTGGC ATCTGTAAAG GAGCTTCTAG TTACATCACA 
CACATTGACT GGGACTCTAG AGGGAAATTA CTGCAAGTTA ATTCAGGTGC CAAAGAACAG 
CTTTTCTTTG AAGCTCCAAG AGGCAGAAAA CACACAATAA GGCCATCAGA GGCTGAGAAG 
ATAGAATGGG ACACCTGGAC CTGTGTCCTG GGGCCCACCT GTGAAGGAAT CTGGCCTGCT 
CACAGCGATG TGACTGACGT CAATGCTGCC AATCTTACCA AAGATGGCTC CCTCTTAGCA 
ACTGGGGATG ACTTTGGTTT TGTGAAGCTT TTTTCATATC CTGTCAAGGG ACAGCATGCA 
AGATTTAAGA AATATGTGGG GCACAGCGCA CACGTTACCA ATGTGAGGTG GCTACACAAT 
GACTCTGTGC TGCTCACTGT AGGAGGTGCT GACACAGCCT TGATGATCTG GACCAGGGAA 
TTTGTGGGGA CGCAGGAGAG CAAGCTGGTT GATAGTGAGG AGTCAGACAC AGATGCAGAA 
GAAGATGGAG GCTATGACAG TGATGTCGCT AGAGAGAAAG CCATTGACTA TACCACCAAG 
ATCTATGCTG TGAGCATCAG GGAAATGGAA GGCACCAAAC CACACCAGCA GCTAAAAGAG 
GTGTCCATGG AAGAAAGGCA GGGAATTGTC AAGGGATCCA GACCACCCGT TAGCCGTGCA 
GCCCCCCAGC CCGAGAAACT CCAGAAGAAC AACATCACCA AGAAAAAGAA GCTGGTTGAG 
GAGCTGGCTC TGGACCACGT GTTTGGCTAC CGAGGATTTG ACTGCCGCAA CAACCTGCAT 
TACCTTAACG ATGGTGCCGA CATCATTTTC CACACTGCGG CAGCTGGCAT TGTGCAGAAC 
CTCTCCACAG GCAGCCAGAG CTTCTACCTG GAGCACACGG ATGACATCCT CTGCCTCACA 
GTGAATCAGC ACCCAAAGTA CAGAAATGTG GTGGCTACCA GCCAGATAGG GACAACACCT 
TCAATTCATA TCTGGGATGC AATGACCAAA CACACCCTTT CCATGCTGCG GTGCTTCCAC 
ACCAAGGGGG TGAATTACAT CAACTTCAGT GCAACCGGGA AGCTCCTGGT GTCTGTGGGC 
GTGGATCCAG AGCACACCAT CACTGTCTGG AGATGGCAAG AAGGTACCAA GGTTGCCAGC 
CGAGGGGGCC ACCTGGAACG AATATTTGTG GTGGAATTTC GCCCTGACTC AGACACACAA 
TTTGTATCTG TTGGAGTCAA ACATATGAAG TTCTGGACTC TGGCAGGAAG TGCTCTGCTG 
TACAAGAAAG GGGTCATCGG GTCTATGGAA GCTGCCAAGA TGCAAACGAT GCTGTCCGTG 
GCTTTTGGTG CTAACAACCT TACTTTTACT GGTGCCATCA ATGGAGATGT CTATGTCTGG 
AAGGAGCACT TCCTCATCCG GCTGGTGGCC AAGGCTCACA CAGGTCCTGT CTTTACAATG 
TACACCACCC TCCGAGATGG GCTTATTGTG ACTGGCGGAA AAGAACGACC GACTAAGGAA 
GGAGGAGCTG TAAAGTTGTG GGACCAGGAG ATGAAGCGCT GTCGGGCCTT CCAGCTGGAA 
ACAGGGCAGC TGGTGGAATG TGTGCGCTCG GTGTGCCGTG GCAAGGGAAA AATCTTAGTA 
GGAACCAAAG ATGGTGAAAT AATTGAAGTT GGGGAAAAAA GCGCTGCTTC AAACATCCTG 
ATTGATGGAC ACATGGAGGG GGAGATCTGG GGCCTAGCCA CACACCCCTC CAAGGACATG 
TTCATCTCAG CCAGCAATGA TGGCACAGCA AGGATCTGGG ACCTGGCTGA CAAGAAACTG 
TTAAACAAGG TGAACCTGGG CCATGCGGCC AGGTGTGCAG CCTATAGCCC TGATGGGGAG 
ATGGTGGCCA TTGGCATGAA GAATGGAGAG TTTGTCATCT TGCTGGTGAA CACCCTGAAA 
GTTTGGGGGA AAAAACGAGA CCGGAAATCT GCCATCCAAG ATATCAGAAT CAGCCCAGAC 
AACAGATTCT TAGCTGTGGG GTCTTCTGAG CAGACAGTTG ACTTCTATGA CCTTACTCAG 
GGCACAAGTC TGAACCGGAT CGGCTACTGC AAAGACATCC CAAGCTTTGT CATTCAGATG 
GACTTTTCTG CAGACAGCAA ATACATTCAG GTGTCAACAG GAGCCTATAA GCGCCAAGTA 
CATGAAGTTC CCCTGGGGAA GCAAGTAACT GAAGCCATGG TTGTTGAGAA GATCACCTGG 
GCTTCCTGGA CAAGTGTTCT CGGAGATGAA GTCATTGGAA TCTGGCCACG AAACGCAGAC 
AAGGCTGATG TCAACTGTGC ATGTGTGACC CATGCTGGCT TGAACATTGT CACAGGGGAT 
GACTTCGGGC TGCTGAAGCT CTTTGATTTT CCATGCACAG AAAAATTCGC CAAGCACAAA 
CGTTACTTTG GTCACTCTGC TCATGTGACG AACATCCGCT TCTCTTCCGA TGACAAGTAT 
GTGGTCAGCA CAGGAGGAGA CGACTGCAGT GTATTTGTGT GGCGATGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514758.1
CDS	234..3827
Translation	MWDYKRFGRTDVSDGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERQGIVKGSRPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_006514695.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514695.4

ORF Insert Sequence:

ATGTGGGACT ACAAGAGATT TGGAAGAACA GATGTATCTG ACGGAAAATG TCTCGTGTCG 
GTTGGTTTAG ACGATTTTCA CAGTGTTGTG TTTTGGGACT GGAAAAAGGG AGAAAAGATA 
GCCACAACCA GGGGACACAA AGACAAAATA TTTGTGGTAA AGTGTAATCC ACAGCATGCT 
GACAAGTTGG TGACAGTTGG GATTAAGCAT ATCAAGTTCT GGCAACAAGC AGGTGGGGGC 
TTCACCTCTA AAAGAGGAAG TTTTGGAAGT GCTGGAAAGT TGGAAACAAT GATGTGTGTT 
TCTTATGGGC GAATGGAAGA TCTGGTGTTC TCAGGTGCAG CCACTGGAGA TATTTTTATC 
TGGAAGGACG TTCTGCTACT GAAGACAGTG AAGGCTCATG ATGGGCCTGT GTTTGCCATG 
TATGCATTAG ACAAGGGCTT TGTAACAGGT GGAAAGGATG GAATTGTGGA ACTCTGGGAT 
GATATGTTTG AAAGATGCTT GAAGACTTAT GCCATCAAAA GGACTGCATT GTCAACCAGC 
TCAAAAGGAC TGCTTTTGGA GGATAACCCT TCAATTCGTG CAATTACCCT AGGGCATGGA 
CACATTCTCG TGGGGACAAA AAATGGAGAG ATTCTAGAAA TTGATAAGAG TGGCCCGATG 
ACCCTGCTTG TTCAGGGACA CATGGAAGGG GAAGTGTGGG GGCTGGCAGC ACACCCGCTC 
CTGCCCATCT GCGCGACAGT AAGTGATGAC AAAACACTTC GGATCTGGGA ATTATCCTCC 
CAGCACCGCA TGCTGGCTGT ACGGAAACTT AAAAAAGGAG GAAGATGTTG TGCTTTCTCC 
CCTGATGGGA AAGCCCTAGC AGTTGGATTG AATGATGGAA GTTTCTTGGT GGTGAATGCT 
GACACAGTGG AAGACATGCT CTCCTTCCAT CACAGAAAAG AAATGATCTC TGATATAAAA 
TTTTCAAAAG ATACAGGAAA ATACCTTGCT GTGGCATCTC ATGATAACTT CGTGGATATC 
TACAACGTGC TTACAAGCAA GAGGGTTGGC ATCTGTAAAG GAGCTTCTAG TTACATCACA 
CACATTGACT GGGACTCTAG AGGGAAATTA CTGCAAGTTA ATTCAGGTGC CAAAGAACAG 
CTTTTCTTTG AAGCTCCAAG AGGCAGAAAA CACACAATAA GGCCATCAGA GGCTGAGAAG 
ATAGAATGGG ACACCTGGAC CTGTGTCCTG GGGCCCACCT GTGAAGGAAT CTGGCCTGCT 
CACAGCGATG TGACTGACGT CAATGCTGCC AATCTTACCA AAGATGGCTC CCTCTTAGCA 
ACTGGGGATG ACTTTGGTTT TGTGAAGCTT TTTTCATATC CTGTCAAGGG ACAGCATGCA 
AGATTTAAGA AATATGTGGG GCACAGCGCA CACGTTACCA ATGTGAGGTG GCTACACAAT 
GACTCTGTGC TGCTCACTGT AGGAGGTGCT GACACAGCCT TGATGATCTG GACCAGGGAA 
TTTGTGGGGA CGCAGGAGAG CAAGCTGGTT GATAGTGAGG AGTCAGACAC AGATGCAGAA 
GAAGATGGAG GCTATGACAG TGATGTCGCT AGAGAGAAAG CCATTGACTA TACCACCAAG 
ATCTATGCTG TGAGCATCAG GGAAATGGAA GGCACCAAAC CACACCAGCA GCTAAAAGAG 
GTGTCCATGG AAGAAAGGCA GGGAATTGTC AAGGGATCCA GACCACCCGT TAGCCGTGCA 
GCCCCCCAGC CCGAGAAACT CCAGAAGAAC AACATCACCA AGAAAAAGAA GCTGGTTGAG 
GAGCTGGCTC TGGACCACGT GTTTGGCTAC CGAGGATTTG ACTGCCGCAA CAACCTGCAT 
TACCTTAACG ATGGTGCCGA CATCATTTTC CACACTGCGG CAGCTGGCAT TGTGCAGAAC 
CTCTCCACAG GCAGCCAGAG CTTCTACCTG GAGCACACGG ATGACATCCT CTGCCTCACA 
GTGAATCAGC ACCCAAAGTA CAGAAATGTG GTGGCTACCA GCCAGATAGG GACAACACCT 
TCAATTCATA TCTGGGATGC AATGACCAAA CACACCCTTT CCATGCTGCG GTGCTTCCAC 
ACCAAGGGGG TGAATTACAT CAACTTCAGT GCAACCGGGA AGCTCCTGGT GTCTGTGGGC 
GTGGATCCAG AGCACACCAT CACTGTCTGG AGATGGCAAG AAGGTACCAA GGTTGCCAGC 
CGAGGGGGCC ACCTGGAACG AATATTTGTG GTGGAATTTC GCCCTGACTC AGACACACAA 
TTTGTATCTG TTGGAGTCAA ACATATGAAG TTCTGGACTC TGGCAGGAAG TGCTCTGCTG 
TACAAGAAAG GGGTCATCGG GTCTATGGAA GCTGCCAAGA TGCAAACGAT GCTGTCCGTG 
GCTTTTGGTG CTAACAACCT TACTTTTACT GGTGCCATCA ATGGAGATGT CTATGTCTGG 
AAGGAGCACT TCCTCATCCG GCTGGTGGCC AAGGCTCACA CAGGTCCTGT CTTTACAATG 
TACACCACCC TCCGAGATGG GCTTATTGTG ACTGGCGGAA AAGAACGACC GACTAAGGAA 
GGAGGAGCTG TAAAGTTGTG GGACCAGGAG ATGAAGCGCT GTCGGGCCTT CCAGCTGGAA 
ACAGGGCAGC TGGTGGAATG TGTGCGCTCG GTGTGCCGTG GCAAGGGAAA AATCTTAGTA 
GGAACCAAAG ATGGTGAAAT AATTGAAGTT GGGGAAAAAA GCGCTGCTTC AAACATCCTG 
ATTGATGGAC ACATGGAGGG GGAGATCTGG GGCCTAGCCA CACACCCCTC CAAGGACATG 
TTCATCTCAG CCAGCAATGA TGGCACAGCA AGGATCTGGG ACCTGGCTGA CAAGAAACTG 
TTAAACAAGG TGAACCTGGG CCATGCGGCC AGGTGTGCAG CCTATAGCCC TGATGGGGAG 
ATGGTGGCCA TTGGCATGAA GAATGGAGAG TTTGTCATCT TGCTGGTGAA CACCCTGAAA 
GTTTGGGGGA AAAAACGAGA CCGGAAATCT GCCATCCAAG ATATCAGAAT CAGCCCAGAC 
AACAGATTCT TAGCTGTGGG GTCTTCTGAG CAGACAGTTG ACTTCTATGA CCTTACTCAG 
GGCACAAGTC TGAACCGGAT CGGCTACTGC AAAGACATCC CAAGCTTTGT CATTCAGATG 
GACTTTTCTG CAGACAGCAA ATACATTCAG GTGTCAACAG GAGCCTATAA GCGCCAAGTA 
CATGAAGTTC CCCTGGGGAA GCAAGTAACT GAAGCCATGG TTGTTGAGAA GATCACCTGG 
GCTTCCTGGA CAAGTGTTCT CGGAGATGAA GTCATTGGAA TCTGGCCACG AAACGCAGAC 
AAGGCTGATG TCAACTGTGC ATGTGTGACC CATGCTGGCT TGAACATTGT CACAGGGGAT 
GACTTCGGGC TGCTGAAGCT CTTTGATTTT CCATGCACAG AAAAATTCGC CAAGCACAAA 
CGTTACTTTG GTCACTCTGC TCATGTGACG AACATCCGCT TCTCTTCCGA TGACAAGTAT 
GTGGTCAGCA CAGGAGGAGA CGACTGCAGT GTATTTGTGT GGCGATGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_006514695.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514758.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu45207
Clone ID Related Accession (Same CDS sequence)	XM_006514695.3 , XM_006514695.4
Accession Version	XM_006514695.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514695.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACT ACAAGAGATT TGGAAGAACA GATGTATCTG ACGGAAAATG TCTCGTGTCG 
GTTGGTTTAG ACGATTTTCA CAGTGTTGTG TTTTGGGACT GGAAAAAGGG AGAAAAGATA 
GCCACAACCA GGGGACACAA AGACAAAATA TTTGTGGTAA AGTGTAATCC ACAGCATGCT 
GACAAGTTGG TGACAGTTGG GATTAAGCAT ATCAAGTTCT GGCAACAAGC AGGTGGGGGC 
TTCACCTCTA AAAGAGGAAG TTTTGGAAGT GCTGGAAAGT TGGAAACAAT GATGTGTGTT 
TCTTATGGGC GAATGGAAGA TCTGGTGTTC TCAGGTGCAG CCACTGGAGA TATTTTTATC 
TGGAAGGACG TTCTGCTACT GAAGACAGTG AAGGCTCATG ATGGGCCTGT GTTTGCCATG 
TATGCATTAG ACAAGGGCTT TGTAACAGGT GGAAAGGATG GAATTGTGGA ACTCTGGGAT 
GATATGTTTG AAAGATGCTT GAAGACTTAT GCCATCAAAA GGACTGCATT GTCAACCAGC 
TCAAAAGGAC TGCTTTTGGA GGATAACCCT TCAATTCGTG CAATTACCCT AGGGCATGGA 
CACATTCTCG TGGGGACAAA AAATGGAGAG ATTCTAGAAA TTGATAAGAG TGGCCCGATG 
ACCCTGCTTG TTCAGGGACA CATGGAAGGG GAAGTGTGGG GGCTGGCAGC ACACCCGCTC 
CTGCCCATCT GCGCGACAGT AAGTGATGAC AAAACACTTC GGATCTGGGA ATTATCCTCC 
CAGCACCGCA TGCTGGCTGT ACGGAAACTT AAAAAAGGAG GAAGATGTTG TGCTTTCTCC 
CCTGATGGGA AAGCCCTAGC AGTTGGATTG AATGATGGAA GTTTCTTGGT GGTGAATGCT 
GACACAGTGG AAGACATGCT CTCCTTCCAT CACAGAAAAG AAATGATCTC TGATATAAAA 
TTTTCAAAAG ATACAGGAAA ATACCTTGCT GTGGCATCTC ATGATAACTT CGTGGATATC 
TACAACGTGC TTACAAGCAA GAGGGTTGGC ATCTGTAAAG GAGCTTCTAG TTACATCACA 
CACATTGACT GGGACTCTAG AGGGAAATTA CTGCAAGTTA ATTCAGGTGC CAAAGAACAG 
CTTTTCTTTG AAGCTCCAAG AGGCAGAAAA CACACAATAA GGCCATCAGA GGCTGAGAAG 
ATAGAATGGG ACACCTGGAC CTGTGTCCTG GGGCCCACCT GTGAAGGAAT CTGGCCTGCT 
CACAGCGATG TGACTGACGT CAATGCTGCC AATCTTACCA AAGATGGCTC CCTCTTAGCA 
ACTGGGGATG ACTTTGGTTT TGTGAAGCTT TTTTCATATC CTGTCAAGGG ACAGCATGCA 
AGATTTAAGA AATATGTGGG GCACAGCGCA CACGTTACCA ATGTGAGGTG GCTACACAAT 
GACTCTGTGC TGCTCACTGT AGGAGGTGCT GACACAGCCT TGATGATCTG GACCAGGGAA 
TTTGTGGGGA CGCAGGAGAG CAAGCTGGTT GATAGTGAGG AGTCAGACAC AGATGCAGAA 
GAAGATGGAG GCTATGACAG TGATGTCGCT AGAGAGAAAG CCATTGACTA TACCACCAAG 
ATCTATGCTG TGAGCATCAG GGAAATGGAA GGCACCAAAC CACACCAGCA GCTAAAAGAG 
GTGTCCATGG AAGAAAGGCA GGGAATTGTC AAGGGATCCA GACCACCCGT TAGCCGTGCA 
GCCCCCCAGC CCGAGAAACT CCAGAAGAAC AACATCACCA AGAAAAAGAA GCTGGTTGAG 
GAGCTGGCTC TGGACCACGT GTTTGGCTAC CGAGGATTTG ACTGCCGCAA CAACCTGCAT 
TACCTTAACG ATGGTGCCGA CATCATTTTC CACACTGCGG CAGCTGGCAT TGTGCAGAAC 
CTCTCCACAG GCAGCCAGAG CTTCTACCTG GAGCACACGG ATGACATCCT CTGCCTCACA 
GTGAATCAGC ACCCAAAGTA CAGAAATGTG GTGGCTACCA GCCAGATAGG GACAACACCT 
TCAATTCATA TCTGGGATGC AATGACCAAA CACACCCTTT CCATGCTGCG GTGCTTCCAC 
ACCAAGGGGG TGAATTACAT CAACTTCAGT GCAACCGGGA AGCTCCTGGT GTCTGTGGGC 
GTGGATCCAG AGCACACCAT CACTGTCTGG AGATGGCAAG AAGGTACCAA GGTTGCCAGC 
CGAGGGGGCC ACCTGGAACG AATATTTGTG GTGGAATTTC GCCCTGACTC AGACACACAA 
TTTGTATCTG TTGGAGTCAA ACATATGAAG TTCTGGACTC TGGCAGGAAG TGCTCTGCTG 
TACAAGAAAG GGGTCATCGG GTCTATGGAA GCTGCCAAGA TGCAAACGAT GCTGTCCGTG 
GCTTTTGGTG CTAACAACCT TACTTTTACT GGTGCCATCA ATGGAGATGT CTATGTCTGG 
AAGGAGCACT TCCTCATCCG GCTGGTGGCC AAGGCTCACA CAGGTCCTGT CTTTACAATG 
TACACCACCC TCCGAGATGG GCTTATTGTG ACTGGCGGAA AAGAACGACC GACTAAGGAA 
GGAGGAGCTG TAAAGTTGTG GGACCAGGAG ATGAAGCGCT GTCGGGCCTT CCAGCTGGAA 
ACAGGGCAGC TGGTGGAATG TGTGCGCTCG GTGTGCCGTG GCAAGGGAAA AATCTTAGTA 
GGAACCAAAG ATGGTGAAAT AATTGAAGTT GGGGAAAAAA GCGCTGCTTC AAACATCCTG 
ATTGATGGAC ACATGGAGGG GGAGATCTGG GGCCTAGCCA CACACCCCTC CAAGGACATG 
TTCATCTCAG CCAGCAATGA TGGCACAGCA AGGATCTGGG ACCTGGCTGA CAAGAAACTG 
TTAAACAAGG TGAACCTGGG CCATGCGGCC AGGTGTGCAG CCTATAGCCC TGATGGGGAG 
ATGGTGGCCA TTGGCATGAA GAATGGAGAG TTTGTCATCT TGCTGGTGAA CACCCTGAAA 
GTTTGGGGGA AAAAACGAGA CCGGAAATCT GCCATCCAAG ATATCAGAAT CAGCCCAGAC 
AACAGATTCT TAGCTGTGGG GTCTTCTGAG CAGACAGTTG ACTTCTATGA CCTTACTCAG 
GGCACAAGTC TGAACCGGAT CGGCTACTGC AAAGACATCC CAAGCTTTGT CATTCAGATG 
GACTTTTCTG CAGACAGCAA ATACATTCAG GTGTCAACAG GAGCCTATAA GCGCCAAGTA 
CATGAAGTTC CCCTGGGGAA GCAAGTAACT GAAGCCATGG TTGTTGAGAA GATCACCTGG 
GCTTCCTGGA CAAGTGTTCT CGGAGATGAA GTCATTGGAA TCTGGCCACG AAACGCAGAC 
AAGGCTGATG TCAACTGTGC ATGTGTGACC CATGCTGGCT TGAACATTGT CACAGGGGAT 
GACTTCGGGC TGCTGAAGCT CTTTGATTTT CCATGCACAG AAAAATTCGC CAAGCACAAA 
CGTTACTTTG GTCACTCTGC TCATGTGACG AACATCCGCT TCTCTTCCGA TGACAAGTAT 
GTGGTCAGCA CAGGAGGAGA CGACTGCAGT GTATTTGTGT GGCGATGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514758.1
CDS	129..3722
Translation	MWDYKRFGRTDVSDGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERQGIVKGSRPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_006514695.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514695.3

ORF Insert Sequence:

ATGTGGGACT ACAAGAGATT TGGAAGAACA GATGTATCTG ACGGAAAATG TCTCGTGTCG 
GTTGGTTTAG ACGATTTTCA CAGTGTTGTG TTTTGGGACT GGAAAAAGGG AGAAAAGATA 
GCCACAACCA GGGGACACAA AGACAAAATA TTTGTGGTAA AGTGTAATCC ACAGCATGCT 
GACAAGTTGG TGACAGTTGG GATTAAGCAT ATCAAGTTCT GGCAACAAGC AGGTGGGGGC 
TTCACCTCTA AAAGAGGAAG TTTTGGAAGT GCTGGAAAGT TGGAAACAAT GATGTGTGTT 
TCTTATGGGC GAATGGAAGA TCTGGTGTTC TCAGGTGCAG CCACTGGAGA TATTTTTATC 
TGGAAGGACG TTCTGCTACT GAAGACAGTG AAGGCTCATG ATGGGCCTGT GTTTGCCATG 
TATGCATTAG ACAAGGGCTT TGTAACAGGT GGAAAGGATG GAATTGTGGA ACTCTGGGAT 
GATATGTTTG AAAGATGCTT GAAGACTTAT GCCATCAAAA GGACTGCATT GTCAACCAGC 
TCAAAAGGAC TGCTTTTGGA GGATAACCCT TCAATTCGTG CAATTACCCT AGGGCATGGA 
CACATTCTCG TGGGGACAAA AAATGGAGAG ATTCTAGAAA TTGATAAGAG TGGCCCGATG 
ACCCTGCTTG TTCAGGGACA CATGGAAGGG GAAGTGTGGG GGCTGGCAGC ACACCCGCTC 
CTGCCCATCT GCGCGACAGT AAGTGATGAC AAAACACTTC GGATCTGGGA ATTATCCTCC 
CAGCACCGCA TGCTGGCTGT ACGGAAACTT AAAAAAGGAG GAAGATGTTG TGCTTTCTCC 
CCTGATGGGA AAGCCCTAGC AGTTGGATTG AATGATGGAA GTTTCTTGGT GGTGAATGCT 
GACACAGTGG AAGACATGCT CTCCTTCCAT CACAGAAAAG AAATGATCTC TGATATAAAA 
TTTTCAAAAG ATACAGGAAA ATACCTTGCT GTGGCATCTC ATGATAACTT CGTGGATATC 
TACAACGTGC TTACAAGCAA GAGGGTTGGC ATCTGTAAAG GAGCTTCTAG TTACATCACA 
CACATTGACT GGGACTCTAG AGGGAAATTA CTGCAAGTTA ATTCAGGTGC CAAAGAACAG 
CTTTTCTTTG AAGCTCCAAG AGGCAGAAAA CACACAATAA GGCCATCAGA GGCTGAGAAG 
ATAGAATGGG ACACCTGGAC CTGTGTCCTG GGGCCCACCT GTGAAGGAAT CTGGCCTGCT 
CACAGCGATG TGACTGACGT CAATGCTGCC AATCTTACCA AAGATGGCTC CCTCTTAGCA 
ACTGGGGATG ACTTTGGTTT TGTGAAGCTT TTTTCATATC CTGTCAAGGG ACAGCATGCA 
AGATTTAAGA AATATGTGGG GCACAGCGCA CACGTTACCA ATGTGAGGTG GCTACACAAT 
GACTCTGTGC TGCTCACTGT AGGAGGTGCT GACACAGCCT TGATGATCTG GACCAGGGAA 
TTTGTGGGGA CGCAGGAGAG CAAGCTGGTT GATAGTGAGG AGTCAGACAC AGATGCAGAA 
GAAGATGGAG GCTATGACAG TGATGTCGCT AGAGAGAAAG CCATTGACTA TACCACCAAG 
ATCTATGCTG TGAGCATCAG GGAAATGGAA GGCACCAAAC CACACCAGCA GCTAAAAGAG 
GTGTCCATGG AAGAAAGGCA GGGAATTGTC AAGGGATCCA GACCACCCGT TAGCCGTGCA 
GCCCCCCAGC CCGAGAAACT CCAGAAGAAC AACATCACCA AGAAAAAGAA GCTGGTTGAG 
GAGCTGGCTC TGGACCACGT GTTTGGCTAC CGAGGATTTG ACTGCCGCAA CAACCTGCAT 
TACCTTAACG ATGGTGCCGA CATCATTTTC CACACTGCGG CAGCTGGCAT TGTGCAGAAC 
CTCTCCACAG GCAGCCAGAG CTTCTACCTG GAGCACACGG ATGACATCCT CTGCCTCACA 
GTGAATCAGC ACCCAAAGTA CAGAAATGTG GTGGCTACCA GCCAGATAGG GACAACACCT 
TCAATTCATA TCTGGGATGC AATGACCAAA CACACCCTTT CCATGCTGCG GTGCTTCCAC 
ACCAAGGGGG TGAATTACAT CAACTTCAGT GCAACCGGGA AGCTCCTGGT GTCTGTGGGC 
GTGGATCCAG AGCACACCAT CACTGTCTGG AGATGGCAAG AAGGTACCAA GGTTGCCAGC 
CGAGGGGGCC ACCTGGAACG AATATTTGTG GTGGAATTTC GCCCTGACTC AGACACACAA 
TTTGTATCTG TTGGAGTCAA ACATATGAAG TTCTGGACTC TGGCAGGAAG TGCTCTGCTG 
TACAAGAAAG GGGTCATCGG GTCTATGGAA GCTGCCAAGA TGCAAACGAT GCTGTCCGTG 
GCTTTTGGTG CTAACAACCT TACTTTTACT GGTGCCATCA ATGGAGATGT CTATGTCTGG 
AAGGAGCACT TCCTCATCCG GCTGGTGGCC AAGGCTCACA CAGGTCCTGT CTTTACAATG 
TACACCACCC TCCGAGATGG GCTTATTGTG ACTGGCGGAA AAGAACGACC GACTAAGGAA 
GGAGGAGCTG TAAAGTTGTG GGACCAGGAG ATGAAGCGCT GTCGGGCCTT CCAGCTGGAA 
ACAGGGCAGC TGGTGGAATG TGTGCGCTCG GTGTGCCGTG GCAAGGGAAA AATCTTAGTA 
GGAACCAAAG ATGGTGAAAT AATTGAAGTT GGGGAAAAAA GCGCTGCTTC AAACATCCTG 
ATTGATGGAC ACATGGAGGG GGAGATCTGG GGCCTAGCCA CACACCCCTC CAAGGACATG 
TTCATCTCAG CCAGCAATGA TGGCACAGCA AGGATCTGGG ACCTGGCTGA CAAGAAACTG 
TTAAACAAGG TGAACCTGGG CCATGCGGCC AGGTGTGCAG CCTATAGCCC TGATGGGGAG 
ATGGTGGCCA TTGGCATGAA GAATGGAGAG TTTGTCATCT TGCTGGTGAA CACCCTGAAA 
GTTTGGGGGA AAAAACGAGA CCGGAAATCT GCCATCCAAG ATATCAGAAT CAGCCCAGAC 
AACAGATTCT TAGCTGTGGG GTCTTCTGAG CAGACAGTTG ACTTCTATGA CCTTACTCAG 
GGCACAAGTC TGAACCGGAT CGGCTACTGC AAAGACATCC CAAGCTTTGT CATTCAGATG 
GACTTTTCTG CAGACAGCAA ATACATTCAG GTGTCAACAG GAGCCTATAA GCGCCAAGTA 
CATGAAGTTC CCCTGGGGAA GCAAGTAACT GAAGCCATGG TTGTTGAGAA GATCACCTGG 
GCTTCCTGGA CAAGTGTTCT CGGAGATGAA GTCATTGGAA TCTGGCCACG AAACGCAGAC 
AAGGCTGATG TCAACTGTGC ATGTGTGACC CATGCTGGCT TGAACATTGT CACAGGGGAT 
GACTTCGGGC TGCTGAAGCT CTTTGATTTT CCATGCACAG AAAAATTCGC CAAGCACAAA 
CGTTACTTTG GTCACTCTGC TCATGTGACG AACATCCGCT TCTCTTCCGA TGACAAGTAT 
GTGGTCAGCA CAGGAGGAGA CGACTGCAGT GTATTTGTGT GGCGATGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_006514694.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514757.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X1, mRNA.

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CloneID	OMu45206
Clone ID Related Accession (Same CDS sequence)	XM_006514694.3 , XM_006514694.4
Accession Version	XM_006514694.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514694.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGGGATTC GTGTGGAGAA AAAGACAAGT AGGTTGACAT CAAATGTGGG ACTACAAGAG 
ATTTGGAAGA ACAGATCTGA CGGAAAATGT CTCGTGTCGG TTGGTTTAGA CGATTTTCAC 
AGTGTTGTGT TTTGGGACTG GAAAAAGGGA GAAAAGATAG CCACAACCAG GGGACACAAA 
GACAAAATAT TTGTGGTAAA GTGTAATCCA CAGCATGCTG ACAAGTTGGT GACAGTTGGG 
ATTAAGCATA TCAAGTTCTG GCAACAAGCA GGTGGGGGCT TCACCTCTAA AAGAGGAAGT 
TTTGGAAGTG CTGGAAAGTT GGAAACAATG ATGTGTGTTT CTTATGGGCG AATGGAAGAT 
CTGGTGTTCT CAGGTGCAGC CACTGGAGAT ATTTTTATCT GGAAGGACGT TCTGCTACTG 
AAGACAGTGA AGGCTCATGA TGGGCCTGTG TTTGCCATGT ATGCATTAGA CAAGGGCTTT 
GTAACAGGTG GAAAGGATGG AATTGTGGAA CTCTGGGATG ATATGTTTGA AAGATGCTTG 
AAGACTTATG CCATCAAAAG GACTGCATTG TCAACCAGCT CAAAAGGACT GCTTTTGGAG 
GATAACCCTT CAATTCGTGC AATTACCCTA GGGCATGGAC ACATTCTCGT GGGGACAAAA 
AATGGAGAGA TTCTAGAAAT TGATAAGAGT GGCCCGATGA CCCTGCTTGT TCAGGGACAC 
ATGGAAGGGG AAGTGTGGGG GCTGGCAGCA CACCCGCTCC TGCCCATCTG CGCGACAGTA 
AGTGATGACA AAACACTTCG GATCTGGGAA TTATCCTCCC AGCACCGCAT GCTGGCTGTA 
CGGAAACTTA AAAAAGGAGG AAGATGTTGT GCTTTCTCCC CTGATGGGAA AGCCCTAGCA 
GTTGGATTGA ATGATGGAAG TTTCTTGGTG GTGAATGCTG ACACAGTGGA AGACATGCTC 
TCCTTCCATC ACAGAAAAGA AATGATCTCT GATATAAAAT TTTCAAAAGA TACAGGAAAA 
TACCTTGCTG TGGCATCTCA TGATAACTTC GTGGATATCT ACAACGTGCT TACAAGCAAG 
AGGGTTGGCA TCTGTAAAGG AGCTTCTAGT TACATCACAC ACATTGACTG GGACTCTAGA 
GGGAAATTAC TGCAAGTTAA TTCAGGTGCC AAAGAACAGC TTTTCTTTGA AGCTCCAAGA 
GGCAGAAAAC ACACAATAAG GCCATCAGAG GCTGAGAAGA TAGAATGGGA CACCTGGACC 
TGTGTCCTGG GGCCCACCTG TGAAGGAATC TGGCCTGCTC ACAGCGATGT GACTGACGTC 
AATGCTGCCA ATCTTACCAA AGATGGCTCC CTCTTAGCAA CTGGGGATGA CTTTGGTTTT 
GTGAAGCTTT TTTCATATCC TGTCAAGGGA CAGCATGCAA GATTTAAGAA ATATGTGGGG 
CACAGCGCAC ACGTTACCAA TGTGAGGTGG CTACACAATG ACTCTGTGCT GCTCACTGTA 
GGAGGTGCTG ACACAGCCTT GATGATCTGG ACCAGGGAAT TTGTGGGGAC GCAGGAGAGC 
AAGCTGGTTG ATAGTGAGGA GTCAGACACA GATGCAGAAG AAGATGGAGG CTATGACAGT 
GATGTCGCTA GAGAGAAAGC CATTGACTAT ACCACCAAGA TCTATGCTGT GAGCATCAGG 
GAAATGGAAG GCACCAAACC ACACCAGCAG CTAAAAGAGG TGTCCATGGA AGAAAGGCAG 
GGAATTGTCA AGGGATCCAG ACCACCCGTT AGCCGTGCAG CCCCCCAGCC CGAGAAACTC 
CAGAAGAACA ACATCACCAA GAAAAAGAAG CTGGTTGAGG AGCTGGCTCT GGACCACGTG 
TTTGGCTACC GAGGATTTGA CTGCCGCAAC AACCTGCATT ACCTTAACGA TGGTGCCGAC 
ATCATTTTCC ACACTGCGGC AGCTGGCATT GTGCAGAACC TCTCCACAGG CAGCCAGAGC 
TTCTACCTGG AGCACACGGA TGACATCCTC TGCCTCACAG TGAATCAGCA CCCAAAGTAC 
AGAAATGTGG TGGCTACCAG CCAGATAGGG ACAACACCTT CAATTCATAT CTGGGATGCA 
ATGACCAAAC ACACCCTTTC CATGCTGCGG TGCTTCCACA CCAAGGGGGT GAATTACATC 
AACTTCAGTG CAACCGGGAA GCTCCTGGTG TCTGTGGGCG TGGATCCAGA GCACACCATC 
ACTGTCTGGA GATGGCAAGA AGGTACCAAG GTTGCCAGCC GAGGGGGCCA CCTGGAACGA 
ATATTTGTGG TGGAATTTCG CCCTGACTCA GACACACAAT TTGTATCTGT TGGAGTCAAA 
CATATGAAGT TCTGGACTCT GGCAGGAAGT GCTCTGCTGT ACAAGAAAGG GGTCATCGGG 
TCTATGGAAG CTGCCAAGAT GCAAACGATG CTGTCCGTGG CTTTTGGTGC TAACAACCTT 
ACTTTTACTG GTGCCATCAA TGGAGATGTC TATGTCTGGA AGGAGCACTT CCTCATCCGG 
CTGGTGGCCA AGGCTCACAC AGGTCCTGTC TTTACAATGT ACACCACCCT CCGAGATGGG 
CTTATTGTGA CTGGCGGAAA AGAACGACCG ACTAAGGAAG GAGGAGCTGT AAAGTTGTGG 
GACCAGGAGA TGAAGCGCTG TCGGGCCTTC CAGCTGGAAA CAGGGCAGCT GGTGGAATGT 
GTGCGCTCGG TGTGCCGTGG CAAGGGAAAA ATCTTAGTAG GAACCAAAGA TGGTGAAATA 
ATTGAAGTTG GGGAAAAAAG CGCTGCTTCA AACATCCTGA TTGATGGACA CATGGAGGGG 
GAGATCTGGG GCCTAGCCAC ACACCCCTCC AAGGACATGT TCATCTCAGC CAGCAATGAT 
GGCACAGCAA GGATCTGGGA CCTGGCTGAC AAGAAACTGT TAAACAAGGT GAACCTGGGC 
CATGCGGCCA GGTGTGCAGC CTATAGCCCT GATGGGGAGA TGGTGGCCAT TGGCATGAAG 
AATGGAGAGT TTGTCATCTT GCTGGTGAAC ACCCTGAAAG TTTGGGGGAA AAAACGAGAC 
CGGAAATCTG CCATCCAAGA TATCAGAATC AGCCCAGACA ACAGATTCTT AGCTGTGGGG 
TCTTCTGAGC AGACAGTTGA CTTCTATGAC CTTACTCAGG GCACAAGTCT GAACCGGATC 
GGCTACTGCA AAGACATCCC AAGCTTTGTC ATTCAGATGG ACTTTTCTGC AGACAGCAAA 
TACATTCAGG TGTCAACAGG AGCCTATAAG CGCCAAGTAC ATGAAGTTCC CCTGGGGAAG 
CAAGTAACTG AAGCCATGGT TGTTGAGAAG ATCACCTGGG CTTCCTGGAC AAGTGTTCTC 
GGAGATGAAG TCATTGGAAT CTGGCCACGA AACGCAGACA AGGCTGATGT CAACTGTGCA 
TGTGTGACCC ATGCTGGCTT GAACATTGTC ACAGGGGATG ACTTCGGGCT GCTGAAGCTC 
TTTGATTTTC CATGCACAGA AAAATTCGCC AAGCACAAAC GTTACTTTGG TCACTCTGCT 
CATGTGACGA ACATCCGCTT CTCTTCCGAT GACAAGTATG TGGTCAGCAC AGGAGGAGAC 
GACTGCAGTG TATTTGTGTG GCGATGTCTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514757.1
CDS	681..6653
Translation	MADRTAPRCQLRLEWVYGYRGHQCRNNLYYTAGKEVVYFVAGVGVVYNTREHSQKFFLGHNDDIISLALHPDKTLIATGQVGKEPYICIWNSYNVHTVSILKDVHTHGVACLAFDSDGQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPSGKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSGIRVEKKTSRLTSNVGLQEIWKNRSDGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERQGIVKGSRPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_006514694.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514694.4

ORF Insert Sequence:

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGGGATTC GTGTGGAGAA AAAGACAAGT AGGTTGACAT CAAATGTGGG ACTACAAGAG 
ATTTGGAAGA ACAGATCTGA CGGAAAATGT CTCGTGTCGG TTGGTTTAGA CGATTTTCAC 
AGTGTTGTGT TTTGGGACTG GAAAAAGGGA GAAAAGATAG CCACAACCAG GGGACACAAA 
GACAAAATAT TTGTGGTAAA GTGTAATCCA CAGCATGCTG ACAAGTTGGT GACAGTTGGG 
ATTAAGCATA TCAAGTTCTG GCAACAAGCA GGTGGGGGCT TCACCTCTAA AAGAGGAAGT 
TTTGGAAGTG CTGGAAAGTT GGAAACAATG ATGTGTGTTT CTTATGGGCG AATGGAAGAT 
CTGGTGTTCT CAGGTGCAGC CACTGGAGAT ATTTTTATCT GGAAGGACGT TCTGCTACTG 
AAGACAGTGA AGGCTCATGA TGGGCCTGTG TTTGCCATGT ATGCATTAGA CAAGGGCTTT 
GTAACAGGTG GAAAGGATGG AATTGTGGAA CTCTGGGATG ATATGTTTGA AAGATGCTTG 
AAGACTTATG CCATCAAAAG GACTGCATTG TCAACCAGCT CAAAAGGACT GCTTTTGGAG 
GATAACCCTT CAATTCGTGC AATTACCCTA GGGCATGGAC ACATTCTCGT GGGGACAAAA 
AATGGAGAGA TTCTAGAAAT TGATAAGAGT GGCCCGATGA CCCTGCTTGT TCAGGGACAC 
ATGGAAGGGG AAGTGTGGGG GCTGGCAGCA CACCCGCTCC TGCCCATCTG CGCGACAGTA 
AGTGATGACA AAACACTTCG GATCTGGGAA TTATCCTCCC AGCACCGCAT GCTGGCTGTA 
CGGAAACTTA AAAAAGGAGG AAGATGTTGT GCTTTCTCCC CTGATGGGAA AGCCCTAGCA 
GTTGGATTGA ATGATGGAAG TTTCTTGGTG GTGAATGCTG ACACAGTGGA AGACATGCTC 
TCCTTCCATC ACAGAAAAGA AATGATCTCT GATATAAAAT TTTCAAAAGA TACAGGAAAA 
TACCTTGCTG TGGCATCTCA TGATAACTTC GTGGATATCT ACAACGTGCT TACAAGCAAG 
AGGGTTGGCA TCTGTAAAGG AGCTTCTAGT TACATCACAC ACATTGACTG GGACTCTAGA 
GGGAAATTAC TGCAAGTTAA TTCAGGTGCC AAAGAACAGC TTTTCTTTGA AGCTCCAAGA 
GGCAGAAAAC ACACAATAAG GCCATCAGAG GCTGAGAAGA TAGAATGGGA CACCTGGACC 
TGTGTCCTGG GGCCCACCTG TGAAGGAATC TGGCCTGCTC ACAGCGATGT GACTGACGTC 
AATGCTGCCA ATCTTACCAA AGATGGCTCC CTCTTAGCAA CTGGGGATGA CTTTGGTTTT 
GTGAAGCTTT TTTCATATCC TGTCAAGGGA CAGCATGCAA GATTTAAGAA ATATGTGGGG 
CACAGCGCAC ACGTTACCAA TGTGAGGTGG CTACACAATG ACTCTGTGCT GCTCACTGTA 
GGAGGTGCTG ACACAGCCTT GATGATCTGG ACCAGGGAAT TTGTGGGGAC GCAGGAGAGC 
AAGCTGGTTG ATAGTGAGGA GTCAGACACA GATGCAGAAG AAGATGGAGG CTATGACAGT 
GATGTCGCTA GAGAGAAAGC CATTGACTAT ACCACCAAGA TCTATGCTGT GAGCATCAGG 
GAAATGGAAG GCACCAAACC ACACCAGCAG CTAAAAGAGG TGTCCATGGA AGAAAGGCAG 
GGAATTGTCA AGGGATCCAG ACCACCCGTT AGCCGTGCAG CCCCCCAGCC CGAGAAACTC 
CAGAAGAACA ACATCACCAA GAAAAAGAAG CTGGTTGAGG AGCTGGCTCT GGACCACGTG 
TTTGGCTACC GAGGATTTGA CTGCCGCAAC AACCTGCATT ACCTTAACGA TGGTGCCGAC 
ATCATTTTCC ACACTGCGGC AGCTGGCATT GTGCAGAACC TCTCCACAGG CAGCCAGAGC 
TTCTACCTGG AGCACACGGA TGACATCCTC TGCCTCACAG TGAATCAGCA CCCAAAGTAC 
AGAAATGTGG TGGCTACCAG CCAGATAGGG ACAACACCTT CAATTCATAT CTGGGATGCA 
ATGACCAAAC ACACCCTTTC CATGCTGCGG TGCTTCCACA CCAAGGGGGT GAATTACATC 
AACTTCAGTG CAACCGGGAA GCTCCTGGTG TCTGTGGGCG TGGATCCAGA GCACACCATC 
ACTGTCTGGA GATGGCAAGA AGGTACCAAG GTTGCCAGCC GAGGGGGCCA CCTGGAACGA 
ATATTTGTGG TGGAATTTCG CCCTGACTCA GACACACAAT TTGTATCTGT TGGAGTCAAA 
CATATGAAGT TCTGGACTCT GGCAGGAAGT GCTCTGCTGT ACAAGAAAGG GGTCATCGGG 
TCTATGGAAG CTGCCAAGAT GCAAACGATG CTGTCCGTGG CTTTTGGTGC TAACAACCTT 
ACTTTTACTG GTGCCATCAA TGGAGATGTC TATGTCTGGA AGGAGCACTT CCTCATCCGG 
CTGGTGGCCA AGGCTCACAC AGGTCCTGTC TTTACAATGT ACACCACCCT CCGAGATGGG 
CTTATTGTGA CTGGCGGAAA AGAACGACCG ACTAAGGAAG GAGGAGCTGT AAAGTTGTGG 
GACCAGGAGA TGAAGCGCTG TCGGGCCTTC CAGCTGGAAA CAGGGCAGCT GGTGGAATGT 
GTGCGCTCGG TGTGCCGTGG CAAGGGAAAA ATCTTAGTAG GAACCAAAGA TGGTGAAATA 
ATTGAAGTTG GGGAAAAAAG CGCTGCTTCA AACATCCTGA TTGATGGACA CATGGAGGGG 
GAGATCTGGG GCCTAGCCAC ACACCCCTCC AAGGACATGT TCATCTCAGC CAGCAATGAT 
GGCACAGCAA GGATCTGGGA CCTGGCTGAC AAGAAACTGT TAAACAAGGT GAACCTGGGC 
CATGCGGCCA GGTGTGCAGC CTATAGCCCT GATGGGGAGA TGGTGGCCAT TGGCATGAAG 
AATGGAGAGT TTGTCATCTT GCTGGTGAAC ACCCTGAAAG TTTGGGGGAA AAAACGAGAC 
CGGAAATCTG CCATCCAAGA TATCAGAATC AGCCCAGACA ACAGATTCTT AGCTGTGGGG 
TCTTCTGAGC AGACAGTTGA CTTCTATGAC CTTACTCAGG GCACAAGTCT GAACCGGATC 
GGCTACTGCA AAGACATCCC AAGCTTTGTC ATTCAGATGG ACTTTTCTGC AGACAGCAAA 
TACATTCAGG TGTCAACAGG AGCCTATAAG CGCCAAGTAC ATGAAGTTCC CCTGGGGAAG 
CAAGTAACTG AAGCCATGGT TGTTGAGAAG ATCACCTGGG CTTCCTGGAC AAGTGTTCTC 
GGAGATGAAG TCATTGGAAT CTGGCCACGA AACGCAGACA AGGCTGATGT CAACTGTGCA 
TGTGTGACCC ATGCTGGCTT GAACATTGTC ACAGGGGATG ACTTCGGGCT GCTGAAGCTC 
TTTGATTTTC CATGCACAGA AAAATTCGCC AAGCACAAAC GTTACTTTGG TCACTCTGCT 
CATGTGACGA ACATCCGCTT CTCTTCCGAT GACAAGTATG TGGTCAGCAC AGGAGGAGAC 
GACTGCAGTG TATTTGTGTG GCGATGTCTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_017314513.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170002.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X2, mRNA.

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CloneID	OMu77887
Clone ID Related Accession (Same CDS sequence)	XM_017314513.1 , XM_017314513.2
Accession Version	XM_017314513.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATGGAAGC CTTTAACAAG TAAAGAAGAA ATCAAGGGGA TTCCCTGGGC CTCCTGGACG 
TGTGTGAGAG GCCCTGAAGT GAGTGGAATT TGGCCCAAAT ACACTGAGGT TATTGACATC 
AATTCAGTGG ACGCCAATTA TAACAGTTCA GTGTTGGTTT CTGGAGATGA TTTTGGACTG 
GTCAAATTGT TCAAATTTCC TTGTCTCAAA AAAGGAGCCA AATTTAGAAA GTATGTGGGC 
CACTCTGCAC ATGTCACCAA CGTCCGTTGG TCCCACGATT TCCAGTGGGT GTTAAGCACA 
GGAGGAGCTG ATCATTCTGT TTTCCAGTGG AGATTTATCC CAGAAGCAGT CAGCAATGGC 
GTGTTGGAAA CCACACCCCA GGAAGGTGGT GCCGACTCCT ATAGTGAGGA ATCTGACTCT 
GACTTCTCTG ACGTTCCGGA ACTGGACTCT GATATTGAGC AGGAAACTCA GATCAATTAC 
GATCGCCAGG TTTACAAAGA AGATCTACCT CAGCTAAAGC AACAAAGTAA AGAGAAAAAC 
CATGCAGTGC CATTCCTGAA GAGGGAGAAG GCTCCTGAGG ATAGCCTGAA ACTGCAGTTC 
ATACATGGTT ACAGAGGCTA TGACTGTAGA AACAATCTGT TCTACACACA AGCTGGAGAA 
GTGGTGTACC ACATTGCTGC AGTTGCTGTG GTATACAACC GGCAACAGCA CGCCCAGAGG 
TTATACCTGG GGCACGATGA TGACATTCTC AGCCTGACCA TCCATCCGGT GAAGGACTAT 
GTAGCCACTG GACAGGTTGG AAGAGATGCT GCTGTTCATG TGTGGGACAC TCAGACTCTA 
AAATGTTTGT CATTACTGAA AGGGCACCAT CAGAGAGGAG TGTGTGCTCT GGACTTTTCA 
GGGATTCGTG TGGAGAAAAA GACAAGTAGG TTGACATCAA ATGTGGGACT ACAAGAGATT 
TGGAAGAACA GATCTGACGG AAAATGTCTC GTGTCGGTTG GTTTAGACGA TTTTCACAGT 
GTTGTGTTTT GGGACTGGAA AAAGGGAGAA AAGATAGCCA CAACCAGGGG ACACAAAGAC 
AAAATATTTG TGGTAAAGTG TAATCCACAG CATGCTGACA AGTTGGTGAC AGTTGGGATT 
AAGCATATCA AGTTCTGGCA ACAAGCAGGT GGGGGCTTCA CCTCTAAAAG AGGAAGTTTT 
GGAAGTGCTG GAAAGTTGGA AACAATGATG TGTGTTTCTT ATGGGCGAAT GGAAGATCTG 
GTGTTCTCAG GTGCAGCCAC TGGAGATATT TTTATCTGGA AGGACGTTCT GCTACTGAAG 
ACAGTGAAGG CTCATGATGG GCCTGTGTTT GCCATGTATG CATTAGACAA GGGCTTTGTA 
ACAGGTGGAA AGGATGGAAT TGTGGAACTC TGGGATGATA TGTTTGAAAG ATGCTTGAAG 
ACTTATGCCA TCAAAAGGAC TGCATTGTCA ACCAGCTCAA AAGGACTGCT TTTGGAGGAT 
AACCCTTCAA TTCGTGCAAT TACCCTAGGG CATGGACACA TTCTCGTGGG GACAAAAAAT 
GGAGAGATTC TAGAAATTGA TAAGAGTGGC CCGATGACCC TGCTTGTTCA GGGACACATG 
GAAGGGGAAG TGTGGGGGCT GGCAGCACAC CCGCTCCTGC CCATCTGCGC GACAGTAAGT 
GATGACAAAA CACTTCGGAT CTGGGAATTA TCCTCCCAGC ACCGCATGCT GGCTGTACGG 
AAACTTAAAA AAGGAGGAAG ATGTTGTGCT TTCTCCCCTG ATGGGAAAGC CCTAGCAGTT 
GGATTGAATG ATGGAAGTTT CTTGGTGGTG AATGCTGACA CAGTGGAAGA CATGCTCTCC 
TTCCATCACA GAAAAGAAAT GATCTCTGAT ATAAAATTTT CAAAAGATAC AGGAAAATAC 
CTTGCTGTGG CATCTCATGA TAACTTCGTG GATATCTACA ACGTGCTTAC AAGCAAGAGG 
GTTGGCATCT GTAAAGGAGC TTCTAGTTAC ATCACACACA TTGACTGGGA CTCTAGAGGG 
AAATTACTGC AAGTTAATTC AGGTGCCAAA GAACAGCTTT TCTTTGAAGC TCCAAGAGGC 
AGAAAACACA CAATAAGGCC ATCAGAGGCT GAGAAGATAG AATGGGACAC CTGGACCTGT 
GTCCTGGGGC CCACCTGTGA AGGAATCTGG CCTGCTCACA GCGATGTGAC TGACGTCAAT 
GCTGCCAATC TTACCAAAGA TGGCTCCCTC TTAGCAACTG GGGATGACTT TGGTTTTGTG 
AAGCTTTTTT CATATCCTGT CAAGGGACAG CATGCAAGAT TTAAGAAATA TGTGGGGCAC 
AGCGCACACG TTACCAATGT GAGGTGGCTA CACAATGACT CTGTGCTGCT CACTGTAGGA 
GGTGCTGACA CAGCCTTGAT GATCTGGACC AGGGAATTTG TGGGGACGCA GGAGAGCAAG 
CTGGTTGATA GTGAGGAGTC AGACACAGAT GCAGAAGAAG ATGGAGGCTA TGACAGTGAT 
GTCGCTAGAG AGAAAGCCAT TGACTATACC ACCAAGATCT ATGCTGTGAG CATCAGGGAA 
ATGGAAGGCA CCAAACCACA CCAGCAGCTA AAAGAGGTGT CCATGGAAGA AAGGCAGGGA 
ATTGTCAAGG GATCCAGACC ACCCGTTAGC CGTGCAGCCC CCCAGCCCGA GAAACTCCAG 
AAGAACAACA TCACCAAGAA AAAGAAGCTG GTTGAGGAGC TGGCTCTGGA CCACGTGTTT 
GGCTACCGAG GATTTGACTG CCGCAACAAC CTGCATTACC TTAACGATGG TGCCGACATC 
ATTTTCCACA CTGCGGCAGC TGGCATTGTG CAGAACCTCT CCACAGGCAG CCAGAGCTTC 
TACCTGGAGC ACACGGATGA CATCCTCTGC CTCACAGTGA ATCAGCACCC AAAGTACAGA 
AATGTGGTGG CTACCAGCCA GATAGGGACA ACACCTTCAA TTCATATCTG GGATGCAATG 
ACCAAACACA CCCTTTCCAT GCTGCGGTGC TTCCACACCA AGGGGGTGAA TTACATCAAC 
TTCAGTGCAA CCGGGAAGCT CCTGGTGTCT GTGGGCGTGG ATCCAGAGCA CACCATCACT 
GTCTGGAGAT GGCAAGAAGG TACCAAGGTT GCCAGCCGAG GGGGCCACCT GGAACGAATA 
TTTGTGGTGG AATTTCGCCC TGACTCAGAC ACACAATTTG TATCTGTTGG AGTCAAACAT 
ATGAAGTTCT GGACTCTGGC AGGAAGTGCT CTGCTGTACA AGAAAGGGGT CATCGGGTCT 
ATGGAAGCTG CCAAGATGCA AACGATGCTG TCCGTGGCTT TTGGTGCTAA CAACCTTACT 
TTTACTGGTG CCATCAATGG AGATGTCTAT GTCTGGAAGG AGCACTTCCT CATCCGGCTG 
GTGGCCAAGG CTCACACAGG TCCTGTCTTT ACAATGTACA CCACCCTCCG AGATGGGCTT 
ATTGTGACTG GCGGAAAAGA ACGACCGACT AAGGAAGGAG GAGCTGTAAA GTTGTGGGAC 
CAGGAGATGA AGCGCTGTCG GGCCTTCCAG CTGGAAACAG GGCAGCTGGT GGAATGTGTG 
CGCTCGGTGT GCCGTGGCAA GGGAAAAATC TTAGTAGGAA CCAAAGATGG TGAAATAATT 
GAAGTTGGGG AAAAAAGCGC TGCTTCAAAC ATCCTGATTG ATGGACACAT GGAGGGGGAG 
ATCTGGGGCC TAGCCACACA CCCCTCCAAG GACATGTTCA TCTCAGCCAG CAATGATGGC 
ACAGCAAGGA TCTGGGACCT GGCTGACAAG AAACTGTTAA ACAAGGTGAA CCTGGGCCAT 
GCGGCCAGGT GTGCAGCCTA TAGCCCTGAT GGGGAGATGG TGGCCATTGG CATGAAGAAT 
GGAGAGTTTG TCATCTTGCT GGTGAACACC CTGAAAGTTT GGGGGAAAAA ACGAGACCGG 
AAATCTGCCA TCCAAGATAT CAGAATCAGC CCAGACAACA GATTCTTAGC TGTGGGGTCT 
TCTGAGCAGA CAGTTGACTT CTATGACCTT ACTCAGGGCA CAAGTCTGAA CCGGATCGGC 
TACTGCAAAG ACATCCCAAG CTTTGTCATT CAGATGGACT TTTCTGCAGA CAGCAAATAC 
ATTCAGGTGT CAACAGGAGC CTATAAGCGC CAAGTACATG AAGTTCCCCT GGGGAAGCAA 
GTAACTGAAG CCATGGTTGT TGAGAAGATC ACCTGGGCTT CCTGGACAAG TGTTCTCGGA 
GATGAAGTCA TTGGAATCTG GCCACGAAAC GCAGACAAGG CTGATGTCAA CTGTGCATGT 
GTGACCCATG CTGGCTTGAA CATTGTCACA GGGGATGACT TCGGGCTGCT GAAGCTCTTT 
GATTTTCCAT GCACAGAAAA ATTCGCCAAG CACAAACGTT ACTTTGGTCA CTCTGCTCAT 
GTGACGAACA TCCGCTTCTC TTCCGATGAC AAGTATGTGG TCAGCACAGG AGGAGACGAC 
TGCAGTGTAT TTGTGTGGCG ATGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170002.1
CDS	671..6640
Translation	MADRTAPRCQLRLEWVYGYRGHQCRNNLYYTAGKEVVYFVAGVGVVYNTREHSQKFFLGHNDDIISLALHPDKTLIATGQVGKEPYICIWNSYNVHTVSILKDVHTHGVACLAFDSDGQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPWKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSGIRVEKKTSRLTSNVGLQEIWKNRSDGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERQGIVKGSRPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_017314513.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314513.1

ORF Insert Sequence:

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATGGAAGC CTTTAACAAG TAAAGAAGAA ATCAAGGGGA TTCCCTGGGC CTCCTGGACG 
TGTGTGAGAG GCCCTGAAGT GAGTGGAATT TGGCCCAAAT ACACTGAGGT TATTGACATC 
AATTCAGTGG ACGCCAATTA TAACAGTTCA GTGTTGGTTT CTGGAGATGA TTTTGGACTG 
GTCAAATTGT TCAAATTTCC TTGTCTCAAA AAAGGAGCCA AATTTAGAAA GTATGTGGGC 
CACTCTGCAC ATGTCACCAA CGTCCGTTGG TCCCACGATT TCCAGTGGGT GTTAAGCACA 
GGAGGAGCTG ATCATTCTGT TTTCCAGTGG AGATTTATCC CAGAAGCAGT CAGCAATGGC 
GTGTTGGAAA CCACACCCCA GGAAGGTGGT GCCGACTCCT ATAGTGAGGA ATCTGACTCT 
GACTTCTCTG ACGTTCCGGA ACTGGACTCT GATATTGAGC AGGAAACTCA GATCAATTAC 
GATCGCCAGG TTTACAAAGA AGATCTACCT CAGCTAAAGC AACAAAGTAA AGAGAAAAAC 
CATGCAGTGC CATTCCTGAA GAGGGAGAAG GCTCCTGAGG ATAGCCTGAA ACTGCAGTTC 
ATACATGGTT ACAGAGGCTA TGACTGTAGA AACAATCTGT TCTACACACA AGCTGGAGAA 
GTGGTGTACC ACATTGCTGC AGTTGCTGTG GTATACAACC GGCAACAGCA CGCCCAGAGG 
TTATACCTGG GGCACGATGA TGACATTCTC AGCCTGACCA TCCATCCGGT GAAGGACTAT 
GTAGCCACTG GACAGGTTGG AAGAGATGCT GCTGTTCATG TGTGGGACAC TCAGACTCTA 
AAATGTTTGT CATTACTGAA AGGGCACCAT CAGAGAGGAG TGTGTGCTCT GGACTTTTCA 
GGGATTCGTG TGGAGAAAAA GACAAGTAGG TTGACATCAA ATGTGGGACT ACAAGAGATT 
TGGAAGAACA GATCTGACGG AAAATGTCTC GTGTCGGTTG GTTTAGACGA TTTTCACAGT 
GTTGTGTTTT GGGACTGGAA AAAGGGAGAA AAGATAGCCA CAACCAGGGG ACACAAAGAC 
AAAATATTTG TGGTAAAGTG TAATCCACAG CATGCTGACA AGTTGGTGAC AGTTGGGATT 
AAGCATATCA AGTTCTGGCA ACAAGCAGGT GGGGGCTTCA CCTCTAAAAG AGGAAGTTTT 
GGAAGTGCTG GAAAGTTGGA AACAATGATG TGTGTTTCTT ATGGGCGAAT GGAAGATCTG 
GTGTTCTCAG GTGCAGCCAC TGGAGATATT TTTATCTGGA AGGACGTTCT GCTACTGAAG 
ACAGTGAAGG CTCATGATGG GCCTGTGTTT GCCATGTATG CATTAGACAA GGGCTTTGTA 
ACAGGTGGAA AGGATGGAAT TGTGGAACTC TGGGATGATA TGTTTGAAAG ATGCTTGAAG 
ACTTATGCCA TCAAAAGGAC TGCATTGTCA ACCAGCTCAA AAGGACTGCT TTTGGAGGAT 
AACCCTTCAA TTCGTGCAAT TACCCTAGGG CATGGACACA TTCTCGTGGG GACAAAAAAT 
GGAGAGATTC TAGAAATTGA TAAGAGTGGC CCGATGACCC TGCTTGTTCA GGGACACATG 
GAAGGGGAAG TGTGGGGGCT GGCAGCACAC CCGCTCCTGC CCATCTGCGC GACAGTAAGT 
GATGACAAAA CACTTCGGAT CTGGGAATTA TCCTCCCAGC ACCGCATGCT GGCTGTACGG 
AAACTTAAAA AAGGAGGAAG ATGTTGTGCT TTCTCCCCTG ATGGGAAAGC CCTAGCAGTT 
GGATTGAATG ATGGAAGTTT CTTGGTGGTG AATGCTGACA CAGTGGAAGA CATGCTCTCC 
TTCCATCACA GAAAAGAAAT GATCTCTGAT ATAAAATTTT CAAAAGATAC AGGAAAATAC 
CTTGCTGTGG CATCTCATGA TAACTTCGTG GATATCTACA ACGTGCTTAC AAGCAAGAGG 
GTTGGCATCT GTAAAGGAGC TTCTAGTTAC ATCACACACA TTGACTGGGA CTCTAGAGGG 
AAATTACTGC AAGTTAATTC AGGTGCCAAA GAACAGCTTT TCTTTGAAGC TCCAAGAGGC 
AGAAAACACA CAATAAGGCC ATCAGAGGCT GAGAAGATAG AATGGGACAC CTGGACCTGT 
GTCCTGGGGC CCACCTGTGA AGGAATCTGG CCTGCTCACA GCGATGTGAC TGACGTCAAT 
GCTGCCAATC TTACCAAAGA TGGCTCCCTC TTAGCAACTG GGGATGACTT TGGTTTTGTG 
AAGCTTTTTT CATATCCTGT CAAGGGACAG CATGCAAGAT TTAAGAAATA TGTGGGGCAC 
AGCGCACACG TTACCAATGT GAGGTGGCTA CACAATGACT CTGTGCTGCT CACTGTAGGA 
GGTGCTGACA CAGCCTTGAT GATCTGGACC AGGGAATTTG TGGGGACGCA GGAGAGCAAG 
CTGGTTGATA GTGAGGAGTC AGACACAGAT GCAGAAGAAG ATGGAGGCTA TGACAGTGAT 
GTCGCTAGAG AGAAAGCCAT TGACTATACC ACCAAGATCT ATGCTGTGAG CATCAGGGAA 
ATGGAAGGCA CCAAACCACA CCAGCAGCTA AAAGAGGTGT CCATGGAAGA AAGGCAGGGA 
ATTGTCAAGG GATCCAGACC ACCCGTTAGC CGTGCAGCCC CCCAGCCCGA GAAACTCCAG 
AAGAACAACA TCACCAAGAA AAAGAAGCTG GTTGAGGAGC TGGCTCTGGA CCACGTGTTT 
GGCTACCGAG GATTTGACTG CCGCAACAAC CTGCATTACC TTAACGATGG TGCCGACATC 
ATTTTCCACA CTGCGGCAGC TGGCATTGTG CAGAACCTCT CCACAGGCAG CCAGAGCTTC 
TACCTGGAGC ACACGGATGA CATCCTCTGC CTCACAGTGA ATCAGCACCC AAAGTACAGA 
AATGTGGTGG CTACCAGCCA GATAGGGACA ACACCTTCAA TTCATATCTG GGATGCAATG 
ACCAAACACA CCCTTTCCAT GCTGCGGTGC TTCCACACCA AGGGGGTGAA TTACATCAAC 
TTCAGTGCAA CCGGGAAGCT CCTGGTGTCT GTGGGCGTGG ATCCAGAGCA CACCATCACT 
GTCTGGAGAT GGCAAGAAGG TACCAAGGTT GCCAGCCGAG GGGGCCACCT GGAACGAATA 
TTTGTGGTGG AATTTCGCCC TGACTCAGAC ACACAATTTG TATCTGTTGG AGTCAAACAT 
ATGAAGTTCT GGACTCTGGC AGGAAGTGCT CTGCTGTACA AGAAAGGGGT CATCGGGTCT 
ATGGAAGCTG CCAAGATGCA AACGATGCTG TCCGTGGCTT TTGGTGCTAA CAACCTTACT 
TTTACTGGTG CCATCAATGG AGATGTCTAT GTCTGGAAGG AGCACTTCCT CATCCGGCTG 
GTGGCCAAGG CTCACACAGG TCCTGTCTTT ACAATGTACA CCACCCTCCG AGATGGGCTT 
ATTGTGACTG GCGGAAAAGA ACGACCGACT AAGGAAGGAG GAGCTGTAAA GTTGTGGGAC 
CAGGAGATGA AGCGCTGTCG GGCCTTCCAG CTGGAAACAG GGCAGCTGGT GGAATGTGTG 
CGCTCGGTGT GCCGTGGCAA GGGAAAAATC TTAGTAGGAA CCAAAGATGG TGAAATAATT 
GAAGTTGGGG AAAAAAGCGC TGCTTCAAAC ATCCTGATTG ATGGACACAT GGAGGGGGAG 
ATCTGGGGCC TAGCCACACA CCCCTCCAAG GACATGTTCA TCTCAGCCAG CAATGATGGC 
ACAGCAAGGA TCTGGGACCT GGCTGACAAG AAACTGTTAA ACAAGGTGAA CCTGGGCCAT 
GCGGCCAGGT GTGCAGCCTA TAGCCCTGAT GGGGAGATGG TGGCCATTGG CATGAAGAAT 
GGAGAGTTTG TCATCTTGCT GGTGAACACC CTGAAAGTTT GGGGGAAAAA ACGAGACCGG 
AAATCTGCCA TCCAAGATAT CAGAATCAGC CCAGACAACA GATTCTTAGC TGTGGGGTCT 
TCTGAGCAGA CAGTTGACTT CTATGACCTT ACTCAGGGCA CAAGTCTGAA CCGGATCGGC 
TACTGCAAAG ACATCCCAAG CTTTGTCATT CAGATGGACT TTTCTGCAGA CAGCAAATAC 
ATTCAGGTGT CAACAGGAGC CTATAAGCGC CAAGTACATG AAGTTCCCCT GGGGAAGCAA 
GTAACTGAAG CCATGGTTGT TGAGAAGATC ACCTGGGCTT CCTGGACAAG TGTTCTCGGA 
GATGAAGTCA TTGGAATCTG GCCACGAAAC GCAGACAAGG CTGATGTCAA CTGTGCATGT 
GTGACCCATG CTGGCTTGAA CATTGTCACA GGGGATGACT TCGGGCTGCT GAAGCTCTTT 
GATTTTCCAT GCACAGAAAA ATTCGCCAAG CACAAACGTT ACTTTGGTCA CTCTGCTCAT 
GTGACGAACA TCCGCTTCTC TTCCGATGAC AAGTATGTGG TCAGCACAGG AGGAGACGAC 
TGCAGTGTAT TTGTGTGGCG ATGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_017314513.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170002.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X2, mRNA.

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CloneID	OMu77887
Clone ID Related Accession (Same CDS sequence)	XM_017314513.1 , XM_017314513.2
Accession Version	XM_017314513.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314513.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATGGAAGC CTTTAACAAG TAAAGAAGAA ATCAAGGGGA TTCCCTGGGC CTCCTGGACG 
TGTGTGAGAG GCCCTGAAGT GAGTGGAATT TGGCCCAAAT ACACTGAGGT TATTGACATC 
AATTCAGTGG ACGCCAATTA TAACAGTTCA GTGTTGGTTT CTGGAGATGA TTTTGGACTG 
GTCAAATTGT TCAAATTTCC TTGTCTCAAA AAAGGAGCCA AATTTAGAAA GTATGTGGGC 
CACTCTGCAC ATGTCACCAA CGTCCGTTGG TCCCACGATT TCCAGTGGGT GTTAAGCACA 
GGAGGAGCTG ATCATTCTGT TTTCCAGTGG AGATTTATCC CAGAAGCAGT CAGCAATGGC 
GTGTTGGAAA CCACACCCCA GGAAGGTGGT GCCGACTCCT ATAGTGAGGA ATCTGACTCT 
GACTTCTCTG ACGTTCCGGA ACTGGACTCT GATATTGAGC AGGAAACTCA GATCAATTAC 
GATCGCCAGG TTTACAAAGA AGATCTACCT CAGCTAAAGC AACAAAGTAA AGAGAAAAAC 
CATGCAGTGC CATTCCTGAA GAGGGAGAAG GCTCCTGAGG ATAGCCTGAA ACTGCAGTTC 
ATACATGGTT ACAGAGGCTA TGACTGTAGA AACAATCTGT TCTACACACA AGCTGGAGAA 
GTGGTGTACC ACATTGCTGC AGTTGCTGTG GTATACAACC GGCAACAGCA CGCCCAGAGG 
TTATACCTGG GGCACGATGA TGACATTCTC AGCCTGACCA TCCATCCGGT GAAGGACTAT 
GTAGCCACTG GACAGGTTGG AAGAGATGCT GCTGTTCATG TGTGGGACAC TCAGACTCTA 
AAATGTTTGT CATTACTGAA AGGGCACCAT CAGAGAGGAG TGTGTGCTCT GGACTTTTCA 
GGGATTCGTG TGGAGAAAAA GACAAGTAGG TTGACATCAA ATGTGGGACT ACAAGAGATT 
TGGAAGAACA GATCTGACGG AAAATGTCTC GTGTCGGTTG GTTTAGACGA TTTTCACAGT 
GTTGTGTTTT GGGACTGGAA AAAGGGAGAA AAGATAGCCA CAACCAGGGG ACACAAAGAC 
AAAATATTTG TGGTAAAGTG TAATCCACAG CATGCTGACA AGTTGGTGAC AGTTGGGATT 
AAGCATATCA AGTTCTGGCA ACAAGCAGGT GGGGGCTTCA CCTCTAAAAG AGGAAGTTTT 
GGAAGTGCTG GAAAGTTGGA AACAATGATG TGTGTTTCTT ATGGGCGAAT GGAAGATCTG 
GTGTTCTCAG GTGCAGCCAC TGGAGATATT TTTATCTGGA AGGACGTTCT GCTACTGAAG 
ACAGTGAAGG CTCATGATGG GCCTGTGTTT GCCATGTATG CATTAGACAA GGGCTTTGTA 
ACAGGTGGAA AGGATGGAAT TGTGGAACTC TGGGATGATA TGTTTGAAAG ATGCTTGAAG 
ACTTATGCCA TCAAAAGGAC TGCATTGTCA ACCAGCTCAA AAGGACTGCT TTTGGAGGAT 
AACCCTTCAA TTCGTGCAAT TACCCTAGGG CATGGACACA TTCTCGTGGG GACAAAAAAT 
GGAGAGATTC TAGAAATTGA TAAGAGTGGC CCGATGACCC TGCTTGTTCA GGGACACATG 
GAAGGGGAAG TGTGGGGGCT GGCAGCACAC CCGCTCCTGC CCATCTGCGC GACAGTAAGT 
GATGACAAAA CACTTCGGAT CTGGGAATTA TCCTCCCAGC ACCGCATGCT GGCTGTACGG 
AAACTTAAAA AAGGAGGAAG ATGTTGTGCT TTCTCCCCTG ATGGGAAAGC CCTAGCAGTT 
GGATTGAATG ATGGAAGTTT CTTGGTGGTG AATGCTGACA CAGTGGAAGA CATGCTCTCC 
TTCCATCACA GAAAAGAAAT GATCTCTGAT ATAAAATTTT CAAAAGATAC AGGAAAATAC 
CTTGCTGTGG CATCTCATGA TAACTTCGTG GATATCTACA ACGTGCTTAC AAGCAAGAGG 
GTTGGCATCT GTAAAGGAGC TTCTAGTTAC ATCACACACA TTGACTGGGA CTCTAGAGGG 
AAATTACTGC AAGTTAATTC AGGTGCCAAA GAACAGCTTT TCTTTGAAGC TCCAAGAGGC 
AGAAAACACA CAATAAGGCC ATCAGAGGCT GAGAAGATAG AATGGGACAC CTGGACCTGT 
GTCCTGGGGC CCACCTGTGA AGGAATCTGG CCTGCTCACA GCGATGTGAC TGACGTCAAT 
GCTGCCAATC TTACCAAAGA TGGCTCCCTC TTAGCAACTG GGGATGACTT TGGTTTTGTG 
AAGCTTTTTT CATATCCTGT CAAGGGACAG CATGCAAGAT TTAAGAAATA TGTGGGGCAC 
AGCGCACACG TTACCAATGT GAGGTGGCTA CACAATGACT CTGTGCTGCT CACTGTAGGA 
GGTGCTGACA CAGCCTTGAT GATCTGGACC AGGGAATTTG TGGGGACGCA GGAGAGCAAG 
CTGGTTGATA GTGAGGAGTC AGACACAGAT GCAGAAGAAG ATGGAGGCTA TGACAGTGAT 
GTCGCTAGAG AGAAAGCCAT TGACTATACC ACCAAGATCT ATGCTGTGAG CATCAGGGAA 
ATGGAAGGCA CCAAACCACA CCAGCAGCTA AAAGAGGTGT CCATGGAAGA AAGGCAGGGA 
ATTGTCAAGG GATCCAGACC ACCCGTTAGC CGTGCAGCCC CCCAGCCCGA GAAACTCCAG 
AAGAACAACA TCACCAAGAA AAAGAAGCTG GTTGAGGAGC TGGCTCTGGA CCACGTGTTT 
GGCTACCGAG GATTTGACTG CCGCAACAAC CTGCATTACC TTAACGATGG TGCCGACATC 
ATTTTCCACA CTGCGGCAGC TGGCATTGTG CAGAACCTCT CCACAGGCAG CCAGAGCTTC 
TACCTGGAGC ACACGGATGA CATCCTCTGC CTCACAGTGA ATCAGCACCC AAAGTACAGA 
AATGTGGTGG CTACCAGCCA GATAGGGACA ACACCTTCAA TTCATATCTG GGATGCAATG 
ACCAAACACA CCCTTTCCAT GCTGCGGTGC TTCCACACCA AGGGGGTGAA TTACATCAAC 
TTCAGTGCAA CCGGGAAGCT CCTGGTGTCT GTGGGCGTGG ATCCAGAGCA CACCATCACT 
GTCTGGAGAT GGCAAGAAGG TACCAAGGTT GCCAGCCGAG GGGGCCACCT GGAACGAATA 
TTTGTGGTGG AATTTCGCCC TGACTCAGAC ACACAATTTG TATCTGTTGG AGTCAAACAT 
ATGAAGTTCT GGACTCTGGC AGGAAGTGCT CTGCTGTACA AGAAAGGGGT CATCGGGTCT 
ATGGAAGCTG CCAAGATGCA AACGATGCTG TCCGTGGCTT TTGGTGCTAA CAACCTTACT 
TTTACTGGTG CCATCAATGG AGATGTCTAT GTCTGGAAGG AGCACTTCCT CATCCGGCTG 
GTGGCCAAGG CTCACACAGG TCCTGTCTTT ACAATGTACA CCACCCTCCG AGATGGGCTT 
ATTGTGACTG GCGGAAAAGA ACGACCGACT AAGGAAGGAG GAGCTGTAAA GTTGTGGGAC 
CAGGAGATGA AGCGCTGTCG GGCCTTCCAG CTGGAAACAG GGCAGCTGGT GGAATGTGTG 
CGCTCGGTGT GCCGTGGCAA GGGAAAAATC TTAGTAGGAA CCAAAGATGG TGAAATAATT 
GAAGTTGGGG AAAAAAGCGC TGCTTCAAAC ATCCTGATTG ATGGACACAT GGAGGGGGAG 
ATCTGGGGCC TAGCCACACA CCCCTCCAAG GACATGTTCA TCTCAGCCAG CAATGATGGC 
ACAGCAAGGA TCTGGGACCT GGCTGACAAG AAACTGTTAA ACAAGGTGAA CCTGGGCCAT 
GCGGCCAGGT GTGCAGCCTA TAGCCCTGAT GGGGAGATGG TGGCCATTGG CATGAAGAAT 
GGAGAGTTTG TCATCTTGCT GGTGAACACC CTGAAAGTTT GGGGGAAAAA ACGAGACCGG 
AAATCTGCCA TCCAAGATAT CAGAATCAGC CCAGACAACA GATTCTTAGC TGTGGGGTCT 
TCTGAGCAGA CAGTTGACTT CTATGACCTT ACTCAGGGCA CAAGTCTGAA CCGGATCGGC 
TACTGCAAAG ACATCCCAAG CTTTGTCATT CAGATGGACT TTTCTGCAGA CAGCAAATAC 
ATTCAGGTGT CAACAGGAGC CTATAAGCGC CAAGTACATG AAGTTCCCCT GGGGAAGCAA 
GTAACTGAAG CCATGGTTGT TGAGAAGATC ACCTGGGCTT CCTGGACAAG TGTTCTCGGA 
GATGAAGTCA TTGGAATCTG GCCACGAAAC GCAGACAAGG CTGATGTCAA CTGTGCATGT 
GTGACCCATG CTGGCTTGAA CATTGTCACA GGGGATGACT TCGGGCTGCT GAAGCTCTTT 
GATTTTCCAT GCACAGAAAA ATTCGCCAAG CACAAACGTT ACTTTGGTCA CTCTGCTCAT 
GTGACGAACA TCCGCTTCTC TTCCGATGAC AAGTATGTGG TCAGCACAGG AGGAGACGAC 
TGCAGTGTAT TTGTGTGGCG ATGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170002.1
CDS	681..6650
Translation	MADRTAPRCQLRLEWVYGYRGHQCRNNLYYTAGKEVVYFVAGVGVVYNTREHSQKFFLGHNDDIISLALHPDKTLIATGQVGKEPYICIWNSYNVHTVSILKDVHTHGVACLAFDSDGQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPWKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSGIRVEKKTSRLTSNVGLQEIWKNRSDGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERQGIVKGSRPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_017314513.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314513.2

ORF Insert Sequence:

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATGGAAGC CTTTAACAAG TAAAGAAGAA ATCAAGGGGA TTCCCTGGGC CTCCTGGACG 
TGTGTGAGAG GCCCTGAAGT GAGTGGAATT TGGCCCAAAT ACACTGAGGT TATTGACATC 
AATTCAGTGG ACGCCAATTA TAACAGTTCA GTGTTGGTTT CTGGAGATGA TTTTGGACTG 
GTCAAATTGT TCAAATTTCC TTGTCTCAAA AAAGGAGCCA AATTTAGAAA GTATGTGGGC 
CACTCTGCAC ATGTCACCAA CGTCCGTTGG TCCCACGATT TCCAGTGGGT GTTAAGCACA 
GGAGGAGCTG ATCATTCTGT TTTCCAGTGG AGATTTATCC CAGAAGCAGT CAGCAATGGC 
GTGTTGGAAA CCACACCCCA GGAAGGTGGT GCCGACTCCT ATAGTGAGGA ATCTGACTCT 
GACTTCTCTG ACGTTCCGGA ACTGGACTCT GATATTGAGC AGGAAACTCA GATCAATTAC 
GATCGCCAGG TTTACAAAGA AGATCTACCT CAGCTAAAGC AACAAAGTAA AGAGAAAAAC 
CATGCAGTGC CATTCCTGAA GAGGGAGAAG GCTCCTGAGG ATAGCCTGAA ACTGCAGTTC 
ATACATGGTT ACAGAGGCTA TGACTGTAGA AACAATCTGT TCTACACACA AGCTGGAGAA 
GTGGTGTACC ACATTGCTGC AGTTGCTGTG GTATACAACC GGCAACAGCA CGCCCAGAGG 
TTATACCTGG GGCACGATGA TGACATTCTC AGCCTGACCA TCCATCCGGT GAAGGACTAT 
GTAGCCACTG GACAGGTTGG AAGAGATGCT GCTGTTCATG TGTGGGACAC TCAGACTCTA 
AAATGTTTGT CATTACTGAA AGGGCACCAT CAGAGAGGAG TGTGTGCTCT GGACTTTTCA 
GGGATTCGTG TGGAGAAAAA GACAAGTAGG TTGACATCAA ATGTGGGACT ACAAGAGATT 
TGGAAGAACA GATCTGACGG AAAATGTCTC GTGTCGGTTG GTTTAGACGA TTTTCACAGT 
GTTGTGTTTT GGGACTGGAA AAAGGGAGAA AAGATAGCCA CAACCAGGGG ACACAAAGAC 
AAAATATTTG TGGTAAAGTG TAATCCACAG CATGCTGACA AGTTGGTGAC AGTTGGGATT 
AAGCATATCA AGTTCTGGCA ACAAGCAGGT GGGGGCTTCA CCTCTAAAAG AGGAAGTTTT 
GGAAGTGCTG GAAAGTTGGA AACAATGATG TGTGTTTCTT ATGGGCGAAT GGAAGATCTG 
GTGTTCTCAG GTGCAGCCAC TGGAGATATT TTTATCTGGA AGGACGTTCT GCTACTGAAG 
ACAGTGAAGG CTCATGATGG GCCTGTGTTT GCCATGTATG CATTAGACAA GGGCTTTGTA 
ACAGGTGGAA AGGATGGAAT TGTGGAACTC TGGGATGATA TGTTTGAAAG ATGCTTGAAG 
ACTTATGCCA TCAAAAGGAC TGCATTGTCA ACCAGCTCAA AAGGACTGCT TTTGGAGGAT 
AACCCTTCAA TTCGTGCAAT TACCCTAGGG CATGGACACA TTCTCGTGGG GACAAAAAAT 
GGAGAGATTC TAGAAATTGA TAAGAGTGGC CCGATGACCC TGCTTGTTCA GGGACACATG 
GAAGGGGAAG TGTGGGGGCT GGCAGCACAC CCGCTCCTGC CCATCTGCGC GACAGTAAGT 
GATGACAAAA CACTTCGGAT CTGGGAATTA TCCTCCCAGC ACCGCATGCT GGCTGTACGG 
AAACTTAAAA AAGGAGGAAG ATGTTGTGCT TTCTCCCCTG ATGGGAAAGC CCTAGCAGTT 
GGATTGAATG ATGGAAGTTT CTTGGTGGTG AATGCTGACA CAGTGGAAGA CATGCTCTCC 
TTCCATCACA GAAAAGAAAT GATCTCTGAT ATAAAATTTT CAAAAGATAC AGGAAAATAC 
CTTGCTGTGG CATCTCATGA TAACTTCGTG GATATCTACA ACGTGCTTAC AAGCAAGAGG 
GTTGGCATCT GTAAAGGAGC TTCTAGTTAC ATCACACACA TTGACTGGGA CTCTAGAGGG 
AAATTACTGC AAGTTAATTC AGGTGCCAAA GAACAGCTTT TCTTTGAAGC TCCAAGAGGC 
AGAAAACACA CAATAAGGCC ATCAGAGGCT GAGAAGATAG AATGGGACAC CTGGACCTGT 
GTCCTGGGGC CCACCTGTGA AGGAATCTGG CCTGCTCACA GCGATGTGAC TGACGTCAAT 
GCTGCCAATC TTACCAAAGA TGGCTCCCTC TTAGCAACTG GGGATGACTT TGGTTTTGTG 
AAGCTTTTTT CATATCCTGT CAAGGGACAG CATGCAAGAT TTAAGAAATA TGTGGGGCAC 
AGCGCACACG TTACCAATGT GAGGTGGCTA CACAATGACT CTGTGCTGCT CACTGTAGGA 
GGTGCTGACA CAGCCTTGAT GATCTGGACC AGGGAATTTG TGGGGACGCA GGAGAGCAAG 
CTGGTTGATA GTGAGGAGTC AGACACAGAT GCAGAAGAAG ATGGAGGCTA TGACAGTGAT 
GTCGCTAGAG AGAAAGCCAT TGACTATACC ACCAAGATCT ATGCTGTGAG CATCAGGGAA 
ATGGAAGGCA CCAAACCACA CCAGCAGCTA AAAGAGGTGT CCATGGAAGA AAGGCAGGGA 
ATTGTCAAGG GATCCAGACC ACCCGTTAGC CGTGCAGCCC CCCAGCCCGA GAAACTCCAG 
AAGAACAACA TCACCAAGAA AAAGAAGCTG GTTGAGGAGC TGGCTCTGGA CCACGTGTTT 
GGCTACCGAG GATTTGACTG CCGCAACAAC CTGCATTACC TTAACGATGG TGCCGACATC 
ATTTTCCACA CTGCGGCAGC TGGCATTGTG CAGAACCTCT CCACAGGCAG CCAGAGCTTC 
TACCTGGAGC ACACGGATGA CATCCTCTGC CTCACAGTGA ATCAGCACCC AAAGTACAGA 
AATGTGGTGG CTACCAGCCA GATAGGGACA ACACCTTCAA TTCATATCTG GGATGCAATG 
ACCAAACACA CCCTTTCCAT GCTGCGGTGC TTCCACACCA AGGGGGTGAA TTACATCAAC 
TTCAGTGCAA CCGGGAAGCT CCTGGTGTCT GTGGGCGTGG ATCCAGAGCA CACCATCACT 
GTCTGGAGAT GGCAAGAAGG TACCAAGGTT GCCAGCCGAG GGGGCCACCT GGAACGAATA 
TTTGTGGTGG AATTTCGCCC TGACTCAGAC ACACAATTTG TATCTGTTGG AGTCAAACAT 
ATGAAGTTCT GGACTCTGGC AGGAAGTGCT CTGCTGTACA AGAAAGGGGT CATCGGGTCT 
ATGGAAGCTG CCAAGATGCA AACGATGCTG TCCGTGGCTT TTGGTGCTAA CAACCTTACT 
TTTACTGGTG CCATCAATGG AGATGTCTAT GTCTGGAAGG AGCACTTCCT CATCCGGCTG 
GTGGCCAAGG CTCACACAGG TCCTGTCTTT ACAATGTACA CCACCCTCCG AGATGGGCTT 
ATTGTGACTG GCGGAAAAGA ACGACCGACT AAGGAAGGAG GAGCTGTAAA GTTGTGGGAC 
CAGGAGATGA AGCGCTGTCG GGCCTTCCAG CTGGAAACAG GGCAGCTGGT GGAATGTGTG 
CGCTCGGTGT GCCGTGGCAA GGGAAAAATC TTAGTAGGAA CCAAAGATGG TGAAATAATT 
GAAGTTGGGG AAAAAAGCGC TGCTTCAAAC ATCCTGATTG ATGGACACAT GGAGGGGGAG 
ATCTGGGGCC TAGCCACACA CCCCTCCAAG GACATGTTCA TCTCAGCCAG CAATGATGGC 
ACAGCAAGGA TCTGGGACCT GGCTGACAAG AAACTGTTAA ACAAGGTGAA CCTGGGCCAT 
GCGGCCAGGT GTGCAGCCTA TAGCCCTGAT GGGGAGATGG TGGCCATTGG CATGAAGAAT 
GGAGAGTTTG TCATCTTGCT GGTGAACACC CTGAAAGTTT GGGGGAAAAA ACGAGACCGG 
AAATCTGCCA TCCAAGATAT CAGAATCAGC CCAGACAACA GATTCTTAGC TGTGGGGTCT 
TCTGAGCAGA CAGTTGACTT CTATGACCTT ACTCAGGGCA CAAGTCTGAA CCGGATCGGC 
TACTGCAAAG ACATCCCAAG CTTTGTCATT CAGATGGACT TTTCTGCAGA CAGCAAATAC 
ATTCAGGTGT CAACAGGAGC CTATAAGCGC CAAGTACATG AAGTTCCCCT GGGGAAGCAA 
GTAACTGAAG CCATGGTTGT TGAGAAGATC ACCTGGGCTT CCTGGACAAG TGTTCTCGGA 
GATGAAGTCA TTGGAATCTG GCCACGAAAC GCAGACAAGG CTGATGTCAA CTGTGCATGT 
GTGACCCATG CTGGCTTGAA CATTGTCACA GGGGATGACT TCGGGCTGCT GAAGCTCTTT 
GATTTTCCAT GCACAGAAAA ATTCGCCAAG CACAAACGTT ACTTTGGTCA CTCTGCTCAT 
GTGACGAACA TCCGCTTCTC TTCCGATGAC AAGTATGTGG TCAGCACAGG AGGAGACGAC 
TGCAGTGTAT TTGTGTGGCG ATGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_017314514.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170003.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X3, mRNA.

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CloneID	OMu77888
Clone ID Related Accession (Same CDS sequence)	XM_017314514.2 , XM_017314514.1
Accession Version	XM_017314514.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314514.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGGGATTC GTGTGGAGAA AAAGACAAGT AGGTTGACAT CAAATGTGGG ACTACAAGAG 
ATTTGGAAGA ACAGATCTGA CGGAAAATGT CTCGTGTCGG TTGGTTTAGA CGATTTTCAC 
AGTGTTGTGT TTTGGGACTG GAAAAAGGGA GAAAAGATAG CCACAACCAG GGGACACAAA 
GACAAAATAT TTGTGGTAAA GTGTAATCCA CAGCATGCTG ACAAGTTGGT GACAGTTGGG 
ATTAAGCATA TCAAGTTCTG GCAACAAGCA GGTGGGGGCT TCACCTCTAA AAGAGGAAGT 
TTTGGAAGTG CTGGAAAGTT GGAAACAATG ATGTGTGTTT CTTATGGGCG AATGGAAGAT 
CTGGTGTTCT CAGGTGCAGC CACTGGAGAT ATTTTTATCT GGAAGGACGT TCTGCTACTG 
AAGACAGTGA AGGCTCATGA TGGGCCTGTG TTTGCCATGT ATGCATTAGA CAAGGGCTTT 
GTAACAGGTG GAAAGGATGG AATTGTGGAA CTCTGGGATG ATATGTTTGA AAGATGCTTG 
AAGACTTATG CCATCAAAAG GACTGCATTG TCAACCAGCT CAAAAGGACT GCTTTTGGAG 
GATAACCCTT CAATTCGTGC AATTACCCTA GGGCATGGAC ACATTCTCGT GGGGACAAAA 
AATGGAGAGA TTCTAGAAAT TGATAAGAGT GGCCCGATGA CCCTGCTTGT TCAGGGACAC 
ATGGAAGGGG AAGTGTGGGG GCTGGCAGCA CACCCGCTCC TGCCCATCTG CGCGACAGTA 
AGTGATGACA AAACACTTCG GATCTGGGAA TTATCCTCCC AGCACCGCAT GCTGGCTGTA 
CGGAAACTTA AAAAAGGAGG AAGATGTTGT GCTTTCTCCC CTGATGGGAA AGCCCTAGCA 
GTTGGATTGA ATGATGGAAG TTTCTTGGTG GTGAATGCTG ACACAGTGGA AGACATGCTC 
TCCTTCCATC ACAGAAAAGA AATGATCTCT GATATAAAAT TTTCAAAAGA TACAGGAAAA 
TACCTTGCTG TGGCATCTCA TGATAACTTC GTGGATATCT ACAACGTGCT TACAAGCAAG 
AGGGTTGGCA TCTGTAAAGG AGCTTCTAGT TACATCACAC ACATTGACTG GGACTCTAGA 
GGGAAATTAC TGCAAGTTAA TTCAGGTGCC AAAGAACAGC TTTTCTTTGA AGCTCCAAGA 
GGCAGAAAAC ACACAATAAG GCCATCAGAG GCTGAGAAGA TAGAATGGGA CACCTGGACC 
TGTGTCCTGG GGCCCACCTG TGAAGGAATC TGGCCTGCTC ACAGCGATGT GACTGACGTC 
AATGCTGCCA ATCTTACCAA AGATGGCTCC CTCTTAGCAA CTGGGGATGA CTTTGGTTTT 
GTGAAGCTTT TTTCATATCC TGTCAAGGGA CAGCATGCAA GATTTAAGAA ATATGTGGGG 
CACAGCGCAC ACGTTACCAA TGTGAGGTGG CTACACAATG ACTCTGTGCT GCTCACTGTA 
GGAGGTGCTG ACACAGCCTT GATGATCTGG ACCAGGGAAT TTGTGGGGAC GCAGGAGAGC 
AAGCTGGTTG ATAGTGAGGA GTCAGACACA GATGCAGAAG AAGATGGAGG CTATGACAGT 
GATGTCGCTA GAGAGAAAGC CATTGACTAT ACCACCAAGA TCTATGCTGT GAGCATCAGG 
GAAATGGAAG GCACCAAACC ACACCAGCAG CTAAAAGAGG TGTCCATGGA AGAAAGGGGA 
TCCAGACCAC CCGTTAGCCG TGCAGCCCCC CAGCCCGAGA AACTCCAGAA GAACAACATC 
ACCAAGAAAA AGAAGCTGGT TGAGGAGCTG GCTCTGGACC ACGTGTTTGG CTACCGAGGA 
TTTGACTGCC GCAACAACCT GCATTACCTT AACGATGGTG CCGACATCAT TTTCCACACT 
GCGGCAGCTG GCATTGTGCA GAACCTCTCC ACAGGCAGCC AGAGCTTCTA CCTGGAGCAC 
ACGGATGACA TCCTCTGCCT CACAGTGAAT CAGCACCCAA AGTACAGAAA TGTGGTGGCT 
ACCAGCCAGA TAGGGACAAC ACCTTCAATT CATATCTGGG ATGCAATGAC CAAACACACC 
CTTTCCATGC TGCGGTGCTT CCACACCAAG GGGGTGAATT ACATCAACTT CAGTGCAACC 
GGGAAGCTCC TGGTGTCTGT GGGCGTGGAT CCAGAGCACA CCATCACTGT CTGGAGATGG 
CAAGAAGGTA CCAAGGTTGC CAGCCGAGGG GGCCACCTGG AACGAATATT TGTGGTGGAA 
TTTCGCCCTG ACTCAGACAC ACAATTTGTA TCTGTTGGAG TCAAACATAT GAAGTTCTGG 
ACTCTGGCAG GAAGTGCTCT GCTGTACAAG AAAGGGGTCA TCGGGTCTAT GGAAGCTGCC 
AAGATGCAAA CGATGCTGTC CGTGGCTTTT GGTGCTAACA ACCTTACTTT TACTGGTGCC 
ATCAATGGAG ATGTCTATGT CTGGAAGGAG CACTTCCTCA TCCGGCTGGT GGCCAAGGCT 
CACACAGGTC CTGTCTTTAC AATGTACACC ACCCTCCGAG ATGGGCTTAT TGTGACTGGC 
GGAAAAGAAC GACCGACTAA GGAAGGAGGA GCTGTAAAGT TGTGGGACCA GGAGATGAAG 
CGCTGTCGGG CCTTCCAGCT GGAAACAGGG CAGCTGGTGG AATGTGTGCG CTCGGTGTGC 
CGTGGCAAGG GAAAAATCTT AGTAGGAACC AAAGATGGTG AAATAATTGA AGTTGGGGAA 
AAAAGCGCTG CTTCAAACAT CCTGATTGAT GGACACATGG AGGGGGAGAT CTGGGGCCTA 
GCCACACACC CCTCCAAGGA CATGTTCATC TCAGCCAGCA ATGATGGCAC AGCAAGGATC 
TGGGACCTGG CTGACAAGAA ACTGTTAAAC AAGGTGAACC TGGGCCATGC GGCCAGGTGT 
GCAGCCTATA GCCCTGATGG GGAGATGGTG GCCATTGGCA TGAAGAATGG AGAGTTTGTC 
ATCTTGCTGG TGAACACCCT GAAAGTTTGG GGGAAAAAAC GAGACCGGAA ATCTGCCATC 
CAAGATATCA GAATCAGCCC AGACAACAGA TTCTTAGCTG TGGGGTCTTC TGAGCAGACA 
GTTGACTTCT ATGACCTTAC TCAGGGCACA AGTCTGAACC GGATCGGCTA CTGCAAAGAC 
ATCCCAAGCT TTGTCATTCA GATGGACTTT TCTGCAGACA GCAAATACAT TCAGGTGTCA 
ACAGGAGCCT ATAAGCGCCA AGTACATGAA GTTCCCCTGG GGAAGCAAGT AACTGAAGCC 
ATGGTTGTTG AGAAGATCAC CTGGGCTTCC TGGACAAGTG TTCTCGGAGA TGAAGTCATT 
GGAATCTGGC CACGAAACGC AGACAAGGCT GATGTCAACT GTGCATGTGT GACCCATGCT 
GGCTTGAACA TTGTCACAGG GGATGACTTC GGGCTGCTGA AGCTCTTTGA TTTTCCATGC 
ACAGAAAAAT TCGCCAAGCA CAAACGTTAC TTTGGTCACT CTGCTCATGT GACGAACATC 
CGCTTCTCTT CCGATGACAA GTATGTGGTC AGCACAGGAG GAGACGACTG CAGTGTATTT 
GTGTGGCGAT GTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170003.1
CDS	681..6638
Translation	MADRTAPRCQLRLEWVYGYRGHQCRNNLYYTAGKEVVYFVAGVGVVYNTREHSQKFFLGHNDDIISLALHPDKTLIATGQVGKEPYICIWNSYNVHTVSILKDVHTHGVACLAFDSDGQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPSGKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSGIRVEKKTSRLTSNVGLQEIWKNRSDGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERGSRPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_017314514.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314514.2

ORF Insert Sequence:

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGGGATTC GTGTGGAGAA AAAGACAAGT AGGTTGACAT CAAATGTGGG ACTACAAGAG 
ATTTGGAAGA ACAGATCTGA CGGAAAATGT CTCGTGTCGG TTGGTTTAGA CGATTTTCAC 
AGTGTTGTGT TTTGGGACTG GAAAAAGGGA GAAAAGATAG CCACAACCAG GGGACACAAA 
GACAAAATAT TTGTGGTAAA GTGTAATCCA CAGCATGCTG ACAAGTTGGT GACAGTTGGG 
ATTAAGCATA TCAAGTTCTG GCAACAAGCA GGTGGGGGCT TCACCTCTAA AAGAGGAAGT 
TTTGGAAGTG CTGGAAAGTT GGAAACAATG ATGTGTGTTT CTTATGGGCG AATGGAAGAT 
CTGGTGTTCT CAGGTGCAGC CACTGGAGAT ATTTTTATCT GGAAGGACGT TCTGCTACTG 
AAGACAGTGA AGGCTCATGA TGGGCCTGTG TTTGCCATGT ATGCATTAGA CAAGGGCTTT 
GTAACAGGTG GAAAGGATGG AATTGTGGAA CTCTGGGATG ATATGTTTGA AAGATGCTTG 
AAGACTTATG CCATCAAAAG GACTGCATTG TCAACCAGCT CAAAAGGACT GCTTTTGGAG 
GATAACCCTT CAATTCGTGC AATTACCCTA GGGCATGGAC ACATTCTCGT GGGGACAAAA 
AATGGAGAGA TTCTAGAAAT TGATAAGAGT GGCCCGATGA CCCTGCTTGT TCAGGGACAC 
ATGGAAGGGG AAGTGTGGGG GCTGGCAGCA CACCCGCTCC TGCCCATCTG CGCGACAGTA 
AGTGATGACA AAACACTTCG GATCTGGGAA TTATCCTCCC AGCACCGCAT GCTGGCTGTA 
CGGAAACTTA AAAAAGGAGG AAGATGTTGT GCTTTCTCCC CTGATGGGAA AGCCCTAGCA 
GTTGGATTGA ATGATGGAAG TTTCTTGGTG GTGAATGCTG ACACAGTGGA AGACATGCTC 
TCCTTCCATC ACAGAAAAGA AATGATCTCT GATATAAAAT TTTCAAAAGA TACAGGAAAA 
TACCTTGCTG TGGCATCTCA TGATAACTTC GTGGATATCT ACAACGTGCT TACAAGCAAG 
AGGGTTGGCA TCTGTAAAGG AGCTTCTAGT TACATCACAC ACATTGACTG GGACTCTAGA 
GGGAAATTAC TGCAAGTTAA TTCAGGTGCC AAAGAACAGC TTTTCTTTGA AGCTCCAAGA 
GGCAGAAAAC ACACAATAAG GCCATCAGAG GCTGAGAAGA TAGAATGGGA CACCTGGACC 
TGTGTCCTGG GGCCCACCTG TGAAGGAATC TGGCCTGCTC ACAGCGATGT GACTGACGTC 
AATGCTGCCA ATCTTACCAA AGATGGCTCC CTCTTAGCAA CTGGGGATGA CTTTGGTTTT 
GTGAAGCTTT TTTCATATCC TGTCAAGGGA CAGCATGCAA GATTTAAGAA ATATGTGGGG 
CACAGCGCAC ACGTTACCAA TGTGAGGTGG CTACACAATG ACTCTGTGCT GCTCACTGTA 
GGAGGTGCTG ACACAGCCTT GATGATCTGG ACCAGGGAAT TTGTGGGGAC GCAGGAGAGC 
AAGCTGGTTG ATAGTGAGGA GTCAGACACA GATGCAGAAG AAGATGGAGG CTATGACAGT 
GATGTCGCTA GAGAGAAAGC CATTGACTAT ACCACCAAGA TCTATGCTGT GAGCATCAGG 
GAAATGGAAG GCACCAAACC ACACCAGCAG CTAAAAGAGG TGTCCATGGA AGAAAGGGGA 
TCCAGACCAC CCGTTAGCCG TGCAGCCCCC CAGCCCGAGA AACTCCAGAA GAACAACATC 
ACCAAGAAAA AGAAGCTGGT TGAGGAGCTG GCTCTGGACC ACGTGTTTGG CTACCGAGGA 
TTTGACTGCC GCAACAACCT GCATTACCTT AACGATGGTG CCGACATCAT TTTCCACACT 
GCGGCAGCTG GCATTGTGCA GAACCTCTCC ACAGGCAGCC AGAGCTTCTA CCTGGAGCAC 
ACGGATGACA TCCTCTGCCT CACAGTGAAT CAGCACCCAA AGTACAGAAA TGTGGTGGCT 
ACCAGCCAGA TAGGGACAAC ACCTTCAATT CATATCTGGG ATGCAATGAC CAAACACACC 
CTTTCCATGC TGCGGTGCTT CCACACCAAG GGGGTGAATT ACATCAACTT CAGTGCAACC 
GGGAAGCTCC TGGTGTCTGT GGGCGTGGAT CCAGAGCACA CCATCACTGT CTGGAGATGG 
CAAGAAGGTA CCAAGGTTGC CAGCCGAGGG GGCCACCTGG AACGAATATT TGTGGTGGAA 
TTTCGCCCTG ACTCAGACAC ACAATTTGTA TCTGTTGGAG TCAAACATAT GAAGTTCTGG 
ACTCTGGCAG GAAGTGCTCT GCTGTACAAG AAAGGGGTCA TCGGGTCTAT GGAAGCTGCC 
AAGATGCAAA CGATGCTGTC CGTGGCTTTT GGTGCTAACA ACCTTACTTT TACTGGTGCC 
ATCAATGGAG ATGTCTATGT CTGGAAGGAG CACTTCCTCA TCCGGCTGGT GGCCAAGGCT 
CACACAGGTC CTGTCTTTAC AATGTACACC ACCCTCCGAG ATGGGCTTAT TGTGACTGGC 
GGAAAAGAAC GACCGACTAA GGAAGGAGGA GCTGTAAAGT TGTGGGACCA GGAGATGAAG 
CGCTGTCGGG CCTTCCAGCT GGAAACAGGG CAGCTGGTGG AATGTGTGCG CTCGGTGTGC 
CGTGGCAAGG GAAAAATCTT AGTAGGAACC AAAGATGGTG AAATAATTGA AGTTGGGGAA 
AAAAGCGCTG CTTCAAACAT CCTGATTGAT GGACACATGG AGGGGGAGAT CTGGGGCCTA 
GCCACACACC CCTCCAAGGA CATGTTCATC TCAGCCAGCA ATGATGGCAC AGCAAGGATC 
TGGGACCTGG CTGACAAGAA ACTGTTAAAC AAGGTGAACC TGGGCCATGC GGCCAGGTGT 
GCAGCCTATA GCCCTGATGG GGAGATGGTG GCCATTGGCA TGAAGAATGG AGAGTTTGTC 
ATCTTGCTGG TGAACACCCT GAAAGTTTGG GGGAAAAAAC GAGACCGGAA ATCTGCCATC 
CAAGATATCA GAATCAGCCC AGACAACAGA TTCTTAGCTG TGGGGTCTTC TGAGCAGACA 
GTTGACTTCT ATGACCTTAC TCAGGGCACA AGTCTGAACC GGATCGGCTA CTGCAAAGAC 
ATCCCAAGCT TTGTCATTCA GATGGACTTT TCTGCAGACA GCAAATACAT TCAGGTGTCA 
ACAGGAGCCT ATAAGCGCCA AGTACATGAA GTTCCCCTGG GGAAGCAAGT AACTGAAGCC 
ATGGTTGTTG AGAAGATCAC CTGGGCTTCC TGGACAAGTG TTCTCGGAGA TGAAGTCATT 
GGAATCTGGC CACGAAACGC AGACAAGGCT GATGTCAACT GTGCATGTGT GACCCATGCT 
GGCTTGAACA TTGTCACAGG GGATGACTTC GGGCTGCTGA AGCTCTTTGA TTTTCCATGC 
ACAGAAAAAT TCGCCAAGCA CAAACGTTAC TTTGGTCACT CTGCTCATGT GACGAACATC 
CGCTTCTCTT CCGATGACAA GTATGTGGTC AGCACAGGAG GAGACGACTG CAGTGTATTT 
GTGTGGCGAT GTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_017314514.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170003.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X3, mRNA.

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CloneID	OMu77888
Clone ID Related Accession (Same CDS sequence)	XM_017314514.2 , XM_017314514.1
Accession Version	XM_017314514.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGGGATTC GTGTGGAGAA AAAGACAAGT AGGTTGACAT CAAATGTGGG ACTACAAGAG 
ATTTGGAAGA ACAGATCTGA CGGAAAATGT CTCGTGTCGG TTGGTTTAGA CGATTTTCAC 
AGTGTTGTGT TTTGGGACTG GAAAAAGGGA GAAAAGATAG CCACAACCAG GGGACACAAA 
GACAAAATAT TTGTGGTAAA GTGTAATCCA CAGCATGCTG ACAAGTTGGT GACAGTTGGG 
ATTAAGCATA TCAAGTTCTG GCAACAAGCA GGTGGGGGCT TCACCTCTAA AAGAGGAAGT 
TTTGGAAGTG CTGGAAAGTT GGAAACAATG ATGTGTGTTT CTTATGGGCG AATGGAAGAT 
CTGGTGTTCT CAGGTGCAGC CACTGGAGAT ATTTTTATCT GGAAGGACGT TCTGCTACTG 
AAGACAGTGA AGGCTCATGA TGGGCCTGTG TTTGCCATGT ATGCATTAGA CAAGGGCTTT 
GTAACAGGTG GAAAGGATGG AATTGTGGAA CTCTGGGATG ATATGTTTGA AAGATGCTTG 
AAGACTTATG CCATCAAAAG GACTGCATTG TCAACCAGCT CAAAAGGACT GCTTTTGGAG 
GATAACCCTT CAATTCGTGC AATTACCCTA GGGCATGGAC ACATTCTCGT GGGGACAAAA 
AATGGAGAGA TTCTAGAAAT TGATAAGAGT GGCCCGATGA CCCTGCTTGT TCAGGGACAC 
ATGGAAGGGG AAGTGTGGGG GCTGGCAGCA CACCCGCTCC TGCCCATCTG CGCGACAGTA 
AGTGATGACA AAACACTTCG GATCTGGGAA TTATCCTCCC AGCACCGCAT GCTGGCTGTA 
CGGAAACTTA AAAAAGGAGG AAGATGTTGT GCTTTCTCCC CTGATGGGAA AGCCCTAGCA 
GTTGGATTGA ATGATGGAAG TTTCTTGGTG GTGAATGCTG ACACAGTGGA AGACATGCTC 
TCCTTCCATC ACAGAAAAGA AATGATCTCT GATATAAAAT TTTCAAAAGA TACAGGAAAA 
TACCTTGCTG TGGCATCTCA TGATAACTTC GTGGATATCT ACAACGTGCT TACAAGCAAG 
AGGGTTGGCA TCTGTAAAGG AGCTTCTAGT TACATCACAC ACATTGACTG GGACTCTAGA 
GGGAAATTAC TGCAAGTTAA TTCAGGTGCC AAAGAACAGC TTTTCTTTGA AGCTCCAAGA 
GGCAGAAAAC ACACAATAAG GCCATCAGAG GCTGAGAAGA TAGAATGGGA CACCTGGACC 
TGTGTCCTGG GGCCCACCTG TGAAGGAATC TGGCCTGCTC ACAGCGATGT GACTGACGTC 
AATGCTGCCA ATCTTACCAA AGATGGCTCC CTCTTAGCAA CTGGGGATGA CTTTGGTTTT 
GTGAAGCTTT TTTCATATCC TGTCAAGGGA CAGCATGCAA GATTTAAGAA ATATGTGGGG 
CACAGCGCAC ACGTTACCAA TGTGAGGTGG CTACACAATG ACTCTGTGCT GCTCACTGTA 
GGAGGTGCTG ACACAGCCTT GATGATCTGG ACCAGGGAAT TTGTGGGGAC GCAGGAGAGC 
AAGCTGGTTG ATAGTGAGGA GTCAGACACA GATGCAGAAG AAGATGGAGG CTATGACAGT 
GATGTCGCTA GAGAGAAAGC CATTGACTAT ACCACCAAGA TCTATGCTGT GAGCATCAGG 
GAAATGGAAG GCACCAAACC ACACCAGCAG CTAAAAGAGG TGTCCATGGA AGAAAGGGGA 
TCCAGACCAC CCGTTAGCCG TGCAGCCCCC CAGCCCGAGA AACTCCAGAA GAACAACATC 
ACCAAGAAAA AGAAGCTGGT TGAGGAGCTG GCTCTGGACC ACGTGTTTGG CTACCGAGGA 
TTTGACTGCC GCAACAACCT GCATTACCTT AACGATGGTG CCGACATCAT TTTCCACACT 
GCGGCAGCTG GCATTGTGCA GAACCTCTCC ACAGGCAGCC AGAGCTTCTA CCTGGAGCAC 
ACGGATGACA TCCTCTGCCT CACAGTGAAT CAGCACCCAA AGTACAGAAA TGTGGTGGCT 
ACCAGCCAGA TAGGGACAAC ACCTTCAATT CATATCTGGG ATGCAATGAC CAAACACACC 
CTTTCCATGC TGCGGTGCTT CCACACCAAG GGGGTGAATT ACATCAACTT CAGTGCAACC 
GGGAAGCTCC TGGTGTCTGT GGGCGTGGAT CCAGAGCACA CCATCACTGT CTGGAGATGG 
CAAGAAGGTA CCAAGGTTGC CAGCCGAGGG GGCCACCTGG AACGAATATT TGTGGTGGAA 
TTTCGCCCTG ACTCAGACAC ACAATTTGTA TCTGTTGGAG TCAAACATAT GAAGTTCTGG 
ACTCTGGCAG GAAGTGCTCT GCTGTACAAG AAAGGGGTCA TCGGGTCTAT GGAAGCTGCC 
AAGATGCAAA CGATGCTGTC CGTGGCTTTT GGTGCTAACA ACCTTACTTT TACTGGTGCC 
ATCAATGGAG ATGTCTATGT CTGGAAGGAG CACTTCCTCA TCCGGCTGGT GGCCAAGGCT 
CACACAGGTC CTGTCTTTAC AATGTACACC ACCCTCCGAG ATGGGCTTAT TGTGACTGGC 
GGAAAAGAAC GACCGACTAA GGAAGGAGGA GCTGTAAAGT TGTGGGACCA GGAGATGAAG 
CGCTGTCGGG CCTTCCAGCT GGAAACAGGG CAGCTGGTGG AATGTGTGCG CTCGGTGTGC 
CGTGGCAAGG GAAAAATCTT AGTAGGAACC AAAGATGGTG AAATAATTGA AGTTGGGGAA 
AAAAGCGCTG CTTCAAACAT CCTGATTGAT GGACACATGG AGGGGGAGAT CTGGGGCCTA 
GCCACACACC CCTCCAAGGA CATGTTCATC TCAGCCAGCA ATGATGGCAC AGCAAGGATC 
TGGGACCTGG CTGACAAGAA ACTGTTAAAC AAGGTGAACC TGGGCCATGC GGCCAGGTGT 
GCAGCCTATA GCCCTGATGG GGAGATGGTG GCCATTGGCA TGAAGAATGG AGAGTTTGTC 
ATCTTGCTGG TGAACACCCT GAAAGTTTGG GGGAAAAAAC GAGACCGGAA ATCTGCCATC 
CAAGATATCA GAATCAGCCC AGACAACAGA TTCTTAGCTG TGGGGTCTTC TGAGCAGACA 
GTTGACTTCT ATGACCTTAC TCAGGGCACA AGTCTGAACC GGATCGGCTA CTGCAAAGAC 
ATCCCAAGCT TTGTCATTCA GATGGACTTT TCTGCAGACA GCAAATACAT TCAGGTGTCA 
ACAGGAGCCT ATAAGCGCCA AGTACATGAA GTTCCCCTGG GGAAGCAAGT AACTGAAGCC 
ATGGTTGTTG AGAAGATCAC CTGGGCTTCC TGGACAAGTG TTCTCGGAGA TGAAGTCATT 
GGAATCTGGC CACGAAACGC AGACAAGGCT GATGTCAACT GTGCATGTGT GACCCATGCT 
GGCTTGAACA TTGTCACAGG GGATGACTTC GGGCTGCTGA AGCTCTTTGA TTTTCCATGC 
ACAGAAAAAT TCGCCAAGCA CAAACGTTAC TTTGGTCACT CTGCTCATGT GACGAACATC 
CGCTTCTCTT CCGATGACAA GTATGTGGTC AGCACAGGAG GAGACGACTG CAGTGTATTT 
GTGTGGCGAT GTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170003.1
CDS	670..6627
Translation	MADRTAPRCQLRLEWVYGYRGHQCRNNLYYTAGKEVVYFVAGVGVVYNTREHSQKFFLGHNDDIISLALHPDKTLIATGQVGKEPYICIWNSYNVHTVSILKDVHTHGVACLAFDSDGQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPSGKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSGIRVEKKTSRLTSNVGLQEIWKNRSDGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERGSRPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_017314514.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314514.1

ORF Insert Sequence:

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGGGATTC GTGTGGAGAA AAAGACAAGT AGGTTGACAT CAAATGTGGG ACTACAAGAG 
ATTTGGAAGA ACAGATCTGA CGGAAAATGT CTCGTGTCGG TTGGTTTAGA CGATTTTCAC 
AGTGTTGTGT TTTGGGACTG GAAAAAGGGA GAAAAGATAG CCACAACCAG GGGACACAAA 
GACAAAATAT TTGTGGTAAA GTGTAATCCA CAGCATGCTG ACAAGTTGGT GACAGTTGGG 
ATTAAGCATA TCAAGTTCTG GCAACAAGCA GGTGGGGGCT TCACCTCTAA AAGAGGAAGT 
TTTGGAAGTG CTGGAAAGTT GGAAACAATG ATGTGTGTTT CTTATGGGCG AATGGAAGAT 
CTGGTGTTCT CAGGTGCAGC CACTGGAGAT ATTTTTATCT GGAAGGACGT TCTGCTACTG 
AAGACAGTGA AGGCTCATGA TGGGCCTGTG TTTGCCATGT ATGCATTAGA CAAGGGCTTT 
GTAACAGGTG GAAAGGATGG AATTGTGGAA CTCTGGGATG ATATGTTTGA AAGATGCTTG 
AAGACTTATG CCATCAAAAG GACTGCATTG TCAACCAGCT CAAAAGGACT GCTTTTGGAG 
GATAACCCTT CAATTCGTGC AATTACCCTA GGGCATGGAC ACATTCTCGT GGGGACAAAA 
AATGGAGAGA TTCTAGAAAT TGATAAGAGT GGCCCGATGA CCCTGCTTGT TCAGGGACAC 
ATGGAAGGGG AAGTGTGGGG GCTGGCAGCA CACCCGCTCC TGCCCATCTG CGCGACAGTA 
AGTGATGACA AAACACTTCG GATCTGGGAA TTATCCTCCC AGCACCGCAT GCTGGCTGTA 
CGGAAACTTA AAAAAGGAGG AAGATGTTGT GCTTTCTCCC CTGATGGGAA AGCCCTAGCA 
GTTGGATTGA ATGATGGAAG TTTCTTGGTG GTGAATGCTG ACACAGTGGA AGACATGCTC 
TCCTTCCATC ACAGAAAAGA AATGATCTCT GATATAAAAT TTTCAAAAGA TACAGGAAAA 
TACCTTGCTG TGGCATCTCA TGATAACTTC GTGGATATCT ACAACGTGCT TACAAGCAAG 
AGGGTTGGCA TCTGTAAAGG AGCTTCTAGT TACATCACAC ACATTGACTG GGACTCTAGA 
GGGAAATTAC TGCAAGTTAA TTCAGGTGCC AAAGAACAGC TTTTCTTTGA AGCTCCAAGA 
GGCAGAAAAC ACACAATAAG GCCATCAGAG GCTGAGAAGA TAGAATGGGA CACCTGGACC 
TGTGTCCTGG GGCCCACCTG TGAAGGAATC TGGCCTGCTC ACAGCGATGT GACTGACGTC 
AATGCTGCCA ATCTTACCAA AGATGGCTCC CTCTTAGCAA CTGGGGATGA CTTTGGTTTT 
GTGAAGCTTT TTTCATATCC TGTCAAGGGA CAGCATGCAA GATTTAAGAA ATATGTGGGG 
CACAGCGCAC ACGTTACCAA TGTGAGGTGG CTACACAATG ACTCTGTGCT GCTCACTGTA 
GGAGGTGCTG ACACAGCCTT GATGATCTGG ACCAGGGAAT TTGTGGGGAC GCAGGAGAGC 
AAGCTGGTTG ATAGTGAGGA GTCAGACACA GATGCAGAAG AAGATGGAGG CTATGACAGT 
GATGTCGCTA GAGAGAAAGC CATTGACTAT ACCACCAAGA TCTATGCTGT GAGCATCAGG 
GAAATGGAAG GCACCAAACC ACACCAGCAG CTAAAAGAGG TGTCCATGGA AGAAAGGGGA 
TCCAGACCAC CCGTTAGCCG TGCAGCCCCC CAGCCCGAGA AACTCCAGAA GAACAACATC 
ACCAAGAAAA AGAAGCTGGT TGAGGAGCTG GCTCTGGACC ACGTGTTTGG CTACCGAGGA 
TTTGACTGCC GCAACAACCT GCATTACCTT AACGATGGTG CCGACATCAT TTTCCACACT 
GCGGCAGCTG GCATTGTGCA GAACCTCTCC ACAGGCAGCC AGAGCTTCTA CCTGGAGCAC 
ACGGATGACA TCCTCTGCCT CACAGTGAAT CAGCACCCAA AGTACAGAAA TGTGGTGGCT 
ACCAGCCAGA TAGGGACAAC ACCTTCAATT CATATCTGGG ATGCAATGAC CAAACACACC 
CTTTCCATGC TGCGGTGCTT CCACACCAAG GGGGTGAATT ACATCAACTT CAGTGCAACC 
GGGAAGCTCC TGGTGTCTGT GGGCGTGGAT CCAGAGCACA CCATCACTGT CTGGAGATGG 
CAAGAAGGTA CCAAGGTTGC CAGCCGAGGG GGCCACCTGG AACGAATATT TGTGGTGGAA 
TTTCGCCCTG ACTCAGACAC ACAATTTGTA TCTGTTGGAG TCAAACATAT GAAGTTCTGG 
ACTCTGGCAG GAAGTGCTCT GCTGTACAAG AAAGGGGTCA TCGGGTCTAT GGAAGCTGCC 
AAGATGCAAA CGATGCTGTC CGTGGCTTTT GGTGCTAACA ACCTTACTTT TACTGGTGCC 
ATCAATGGAG ATGTCTATGT CTGGAAGGAG CACTTCCTCA TCCGGCTGGT GGCCAAGGCT 
CACACAGGTC CTGTCTTTAC AATGTACACC ACCCTCCGAG ATGGGCTTAT TGTGACTGGC 
GGAAAAGAAC GACCGACTAA GGAAGGAGGA GCTGTAAAGT TGTGGGACCA GGAGATGAAG 
CGCTGTCGGG CCTTCCAGCT GGAAACAGGG CAGCTGGTGG AATGTGTGCG CTCGGTGTGC 
CGTGGCAAGG GAAAAATCTT AGTAGGAACC AAAGATGGTG AAATAATTGA AGTTGGGGAA 
AAAAGCGCTG CTTCAAACAT CCTGATTGAT GGACACATGG AGGGGGAGAT CTGGGGCCTA 
GCCACACACC CCTCCAAGGA CATGTTCATC TCAGCCAGCA ATGATGGCAC AGCAAGGATC 
TGGGACCTGG CTGACAAGAA ACTGTTAAAC AAGGTGAACC TGGGCCATGC GGCCAGGTGT 
GCAGCCTATA GCCCTGATGG GGAGATGGTG GCCATTGGCA TGAAGAATGG AGAGTTTGTC 
ATCTTGCTGG TGAACACCCT GAAAGTTTGG GGGAAAAAAC GAGACCGGAA ATCTGCCATC 
CAAGATATCA GAATCAGCCC AGACAACAGA TTCTTAGCTG TGGGGTCTTC TGAGCAGACA 
GTTGACTTCT ATGACCTTAC TCAGGGCACA AGTCTGAACC GGATCGGCTA CTGCAAAGAC 
ATCCCAAGCT TTGTCATTCA GATGGACTTT TCTGCAGACA GCAAATACAT TCAGGTGTCA 
ACAGGAGCCT ATAAGCGCCA AGTACATGAA GTTCCCCTGG GGAAGCAAGT AACTGAAGCC 
ATGGTTGTTG AGAAGATCAC CTGGGCTTCC TGGACAAGTG TTCTCGGAGA TGAAGTCATT 
GGAATCTGGC CACGAAACGC AGACAAGGCT GATGTCAACT GTGCATGTGT GACCCATGCT 
GGCTTGAACA TTGTCACAGG GGATGACTTC GGGCTGCTGA AGCTCTTTGA TTTTCCATGC 
ACAGAAAAAT TCGCCAAGCA CAAACGTTAC TTTGGTCACT CTGCTCATGT GACGAACATC 
CGCTTCTCTT CCGATGACAA GTATGTGGTC AGCACAGGAG GAGACGACTG CAGTGTATTT 
GTGTGGCGAT GTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_017314515.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170004.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X4, mRNA.

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CloneID	OMu77889
Clone ID Related Accession (Same CDS sequence)	XM_017314515.1 , XM_017314515.2
Accession Version	XM_017314515.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGGGATTC GTGTGGAGAA AAAGACAAGT AGGTTGACAT CAAATGTGGG ACTACAAGAG 
ATTTGGAAGA ACAGATCTGA CGGAAAATGT CTCGTGTCGG TTGGTTTAGA CGATTTTCAC 
AGTGTTGTGT TTTGGGACTG GAAAAAGGGA GAAAAGATAG CCACAACCAG GGGACACAAA 
GACAAAATAT TTGTGGTAAA GTGTAATCCA CAGCATGCTG ACAAGTTGGT GACAGTTGGG 
ATTAAGCATA TCAAGTTCTG GCAACAAGCA GGTGGGGGCT TCACCTCTAA AAGAGGAAGT 
TTTGGAAGTG CTGGAAAGTT GGAAACAATG ATGTGTGTTT CTTATGGGCG AATGGAAGAT 
CTGGTGTTCT CAGGTGCAGC CACTGGAGAT ATTTTTATCT GGAAGGACGT TCTGCTACTG 
AAGACAGTGA AGGCTCATGA TGGGCCTGTG TTTGCCATGT ATGCATTAGA CAAGGGCTTT 
GTAACAGGTG GAAAGGATGG AATTGTGGAA CTCTGGGATG ATATGTTTGA AAGATGCTTG 
AAGACTTATG CCATCAAAAG GACTGCATTG TCAACCAGCT CAAAAGGACT GCTTTTGGAG 
GATAACCCTT CAATTCGTGC AATTACCCTA GGGCATGGAC ACATTCTCGT GGGGACAAAA 
AATGGAGAGA TTCTAGAAAT TGATAAGAGT GGCCCGATGA CCCTGCTTGT TCAGGGACAC 
ATGGAAGGGG AAGTGTGGGG GCTGGCAGCA CACCCGCTCC TGCCCATCTG CGCGACAGTA 
AGTGATGACA AAACACTTCG GATCTGGGAA TTATCCTCCC AGCACCGCAT GCTGGCTGTA 
CGGAAACTTA AAAAAGGAGG AAGATGTTGT GCTTTCTCCC CTGATGGGAA AGCCCTAGCA 
GTTGGATTGA ATGATGGAAG TTTCTTGGTG GTGAATGCTG ACACAGTGGA AGACATGCTC 
TCCTTCCATC ACAGAAAAGA AATGATCTCT GATATAAAAT TTTCAAAAGA TACAGGAAAA 
TACCTTGCTG TGGCATCTCA TGATAACTTC GTGGATATCT ACAACGTGCT TACAAGCAAG 
AGGGTTGGCA TCTGTAAAGG AGCTTCTAGT TACATCACAC ACATTGACTG GGACTCTAGA 
GGGAAATTAC TGCAAGTTAA TTCAGGTGCC AAAGAACAGC TTTTCTTTGA AGCTCCAAGA 
GGCAGAAAAC ACACAATAAG GCCATCAGAG GCTGAGAAGA TAGAATGGGA CACCTGGACC 
TGTGTCCTGG GGCCCACCTG TGAAGGAATC TGGCCTGCTC ACAGCGATGT GACTGACGTC 
AATGCTGCCA ATCTTACCAA AGATGGCTCC CTCTTAGCAA CTGGGGATGA CTTTGGTTTT 
GTGAAGCTTT TTTCATATCC TGTCAAGGGA CAGCATGCAA GATTTAAGAA ATATGTGGGG 
CACAGCGCAC ACGTTACCAA TGTGAGGTGG CTACACAATG ACTCTGTGCT GCTCACTGTA 
GGAGGTGCTG ACACAGCCTT GATGATCTGG ACCAGGGAAT TTGTGGGGAC GCAGGAGAGC 
AAGCTGGTTG ATAGTGAGGA GTCAGACACA GATGCAGAAG AAGATGGAGG CTATGACAGT 
GATGTCGCTA GAGAGAAAGC CATTGACTAT ACCACCAAGA TCTATGCTGT GAGCATCAGG 
GAAATGGAAG GCACCAAACC ACACCAGCAG CTAAAAGAGG TGTCCATGGA AGAAAGACCA 
CCCGTTAGCC GTGCAGCCCC CCAGCCCGAG AAACTCCAGA AGAACAACAT CACCAAGAAA 
AAGAAGCTGG TTGAGGAGCT GGCTCTGGAC CACGTGTTTG GCTACCGAGG ATTTGACTGC 
CGCAACAACC TGCATTACCT TAACGATGGT GCCGACATCA TTTTCCACAC TGCGGCAGCT 
GGCATTGTGC AGAACCTCTC CACAGGCAGC CAGAGCTTCT ACCTGGAGCA CACGGATGAC 
ATCCTCTGCC TCACAGTGAA TCAGCACCCA AAGTACAGAA ATGTGGTGGC TACCAGCCAG 
ATAGGGACAA CACCTTCAAT TCATATCTGG GATGCAATGA CCAAACACAC CCTTTCCATG 
CTGCGGTGCT TCCACACCAA GGGGGTGAAT TACATCAACT TCAGTGCAAC CGGGAAGCTC 
CTGGTGTCTG TGGGCGTGGA TCCAGAGCAC ACCATCACTG TCTGGAGATG GCAAGAAGGT 
ACCAAGGTTG CCAGCCGAGG GGGCCACCTG GAACGAATAT TTGTGGTGGA ATTTCGCCCT 
GACTCAGACA CACAATTTGT ATCTGTTGGA GTCAAACATA TGAAGTTCTG GACTCTGGCA 
GGAAGTGCTC TGCTGTACAA GAAAGGGGTC ATCGGGTCTA TGGAAGCTGC CAAGATGCAA 
ACGATGCTGT CCGTGGCTTT TGGTGCTAAC AACCTTACTT TTACTGGTGC CATCAATGGA 
GATGTCTATG TCTGGAAGGA GCACTTCCTC ATCCGGCTGG TGGCCAAGGC TCACACAGGT 
CCTGTCTTTA CAATGTACAC CACCCTCCGA GATGGGCTTA TTGTGACTGG CGGAAAAGAA 
CGACCGACTA AGGAAGGAGG AGCTGTAAAG TTGTGGGACC AGGAGATGAA GCGCTGTCGG 
GCCTTCCAGC TGGAAACAGG GCAGCTGGTG GAATGTGTGC GCTCGGTGTG CCGTGGCAAG 
GGAAAAATCT TAGTAGGAAC CAAAGATGGT GAAATAATTG AAGTTGGGGA AAAAAGCGCT 
GCTTCAAACA TCCTGATTGA TGGACACATG GAGGGGGAGA TCTGGGGCCT AGCCACACAC 
CCCTCCAAGG ACATGTTCAT CTCAGCCAGC AATGATGGCA CAGCAAGGAT CTGGGACCTG 
GCTGACAAGA AACTGTTAAA CAAGGTGAAC CTGGGCCATG CGGCCAGGTG TGCAGCCTAT 
AGCCCTGATG GGGAGATGGT GGCCATTGGC ATGAAGAATG GAGAGTTTGT CATCTTGCTG 
GTGAACACCC TGAAAGTTTG GGGGAAAAAA CGAGACCGGA AATCTGCCAT CCAAGATATC 
AGAATCAGCC CAGACAACAG ATTCTTAGCT GTGGGGTCTT CTGAGCAGAC AGTTGACTTC 
TATGACCTTA CTCAGGGCAC AAGTCTGAAC CGGATCGGCT ACTGCAAAGA CATCCCAAGC 
TTTGTCATTC AGATGGACTT TTCTGCAGAC AGCAAATACA TTCAGGTGTC AACAGGAGCC 
TATAAGCGCC AAGTACATGA AGTTCCCCTG GGGAAGCAAG TAACTGAAGC CATGGTTGTT 
GAGAAGATCA CCTGGGCTTC CTGGACAAGT GTTCTCGGAG ATGAAGTCAT TGGAATCTGG 
CCACGAAACG CAGACAAGGC TGATGTCAAC TGTGCATGTG TGACCCATGC TGGCTTGAAC 
ATTGTCACAG GGGATGACTT CGGGCTGCTG AAGCTCTTTG ATTTTCCATG CACAGAAAAA 
TTCGCCAAGC ACAAACGTTA CTTTGGTCAC TCTGCTCATG TGACGAACAT CCGCTTCTCT 
TCCGATGACA AGTATGTGGT CAGCACAGGA GGAGACGACT GCAGTGTATT TGTGTGGCGA 
TGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170004.1
CDS	670..6618
Translation	MADRTAPRCQLRLEWVYGYRGHQCRNNLYYTAGKEVVYFVAGVGVVYNTREHSQKFFLGHNDDIISLALHPDKTLIATGQVGKEPYICIWNSYNVHTVSILKDVHTHGVACLAFDSDGQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPSGKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSGIRVEKKTSRLTSNVGLQEIWKNRSDGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_017314515.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314515.1

ORF Insert Sequence:

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGGGATTC GTGTGGAGAA AAAGACAAGT AGGTTGACAT CAAATGTGGG ACTACAAGAG 
ATTTGGAAGA ACAGATCTGA CGGAAAATGT CTCGTGTCGG TTGGTTTAGA CGATTTTCAC 
AGTGTTGTGT TTTGGGACTG GAAAAAGGGA GAAAAGATAG CCACAACCAG GGGACACAAA 
GACAAAATAT TTGTGGTAAA GTGTAATCCA CAGCATGCTG ACAAGTTGGT GACAGTTGGG 
ATTAAGCATA TCAAGTTCTG GCAACAAGCA GGTGGGGGCT TCACCTCTAA AAGAGGAAGT 
TTTGGAAGTG CTGGAAAGTT GGAAACAATG ATGTGTGTTT CTTATGGGCG AATGGAAGAT 
CTGGTGTTCT CAGGTGCAGC CACTGGAGAT ATTTTTATCT GGAAGGACGT TCTGCTACTG 
AAGACAGTGA AGGCTCATGA TGGGCCTGTG TTTGCCATGT ATGCATTAGA CAAGGGCTTT 
GTAACAGGTG GAAAGGATGG AATTGTGGAA CTCTGGGATG ATATGTTTGA AAGATGCTTG 
AAGACTTATG CCATCAAAAG GACTGCATTG TCAACCAGCT CAAAAGGACT GCTTTTGGAG 
GATAACCCTT CAATTCGTGC AATTACCCTA GGGCATGGAC ACATTCTCGT GGGGACAAAA 
AATGGAGAGA TTCTAGAAAT TGATAAGAGT GGCCCGATGA CCCTGCTTGT TCAGGGACAC 
ATGGAAGGGG AAGTGTGGGG GCTGGCAGCA CACCCGCTCC TGCCCATCTG CGCGACAGTA 
AGTGATGACA AAACACTTCG GATCTGGGAA TTATCCTCCC AGCACCGCAT GCTGGCTGTA 
CGGAAACTTA AAAAAGGAGG AAGATGTTGT GCTTTCTCCC CTGATGGGAA AGCCCTAGCA 
GTTGGATTGA ATGATGGAAG TTTCTTGGTG GTGAATGCTG ACACAGTGGA AGACATGCTC 
TCCTTCCATC ACAGAAAAGA AATGATCTCT GATATAAAAT TTTCAAAAGA TACAGGAAAA 
TACCTTGCTG TGGCATCTCA TGATAACTTC GTGGATATCT ACAACGTGCT TACAAGCAAG 
AGGGTTGGCA TCTGTAAAGG AGCTTCTAGT TACATCACAC ACATTGACTG GGACTCTAGA 
GGGAAATTAC TGCAAGTTAA TTCAGGTGCC AAAGAACAGC TTTTCTTTGA AGCTCCAAGA 
GGCAGAAAAC ACACAATAAG GCCATCAGAG GCTGAGAAGA TAGAATGGGA CACCTGGACC 
TGTGTCCTGG GGCCCACCTG TGAAGGAATC TGGCCTGCTC ACAGCGATGT GACTGACGTC 
AATGCTGCCA ATCTTACCAA AGATGGCTCC CTCTTAGCAA CTGGGGATGA CTTTGGTTTT 
GTGAAGCTTT TTTCATATCC TGTCAAGGGA CAGCATGCAA GATTTAAGAA ATATGTGGGG 
CACAGCGCAC ACGTTACCAA TGTGAGGTGG CTACACAATG ACTCTGTGCT GCTCACTGTA 
GGAGGTGCTG ACACAGCCTT GATGATCTGG ACCAGGGAAT TTGTGGGGAC GCAGGAGAGC 
AAGCTGGTTG ATAGTGAGGA GTCAGACACA GATGCAGAAG AAGATGGAGG CTATGACAGT 
GATGTCGCTA GAGAGAAAGC CATTGACTAT ACCACCAAGA TCTATGCTGT GAGCATCAGG 
GAAATGGAAG GCACCAAACC ACACCAGCAG CTAAAAGAGG TGTCCATGGA AGAAAGACCA 
CCCGTTAGCC GTGCAGCCCC CCAGCCCGAG AAACTCCAGA AGAACAACAT CACCAAGAAA 
AAGAAGCTGG TTGAGGAGCT GGCTCTGGAC CACGTGTTTG GCTACCGAGG ATTTGACTGC 
CGCAACAACC TGCATTACCT TAACGATGGT GCCGACATCA TTTTCCACAC TGCGGCAGCT 
GGCATTGTGC AGAACCTCTC CACAGGCAGC CAGAGCTTCT ACCTGGAGCA CACGGATGAC 
ATCCTCTGCC TCACAGTGAA TCAGCACCCA AAGTACAGAA ATGTGGTGGC TACCAGCCAG 
ATAGGGACAA CACCTTCAAT TCATATCTGG GATGCAATGA CCAAACACAC CCTTTCCATG 
CTGCGGTGCT TCCACACCAA GGGGGTGAAT TACATCAACT TCAGTGCAAC CGGGAAGCTC 
CTGGTGTCTG TGGGCGTGGA TCCAGAGCAC ACCATCACTG TCTGGAGATG GCAAGAAGGT 
ACCAAGGTTG CCAGCCGAGG GGGCCACCTG GAACGAATAT TTGTGGTGGA ATTTCGCCCT 
GACTCAGACA CACAATTTGT ATCTGTTGGA GTCAAACATA TGAAGTTCTG GACTCTGGCA 
GGAAGTGCTC TGCTGTACAA GAAAGGGGTC ATCGGGTCTA TGGAAGCTGC CAAGATGCAA 
ACGATGCTGT CCGTGGCTTT TGGTGCTAAC AACCTTACTT TTACTGGTGC CATCAATGGA 
GATGTCTATG TCTGGAAGGA GCACTTCCTC ATCCGGCTGG TGGCCAAGGC TCACACAGGT 
CCTGTCTTTA CAATGTACAC CACCCTCCGA GATGGGCTTA TTGTGACTGG CGGAAAAGAA 
CGACCGACTA AGGAAGGAGG AGCTGTAAAG TTGTGGGACC AGGAGATGAA GCGCTGTCGG 
GCCTTCCAGC TGGAAACAGG GCAGCTGGTG GAATGTGTGC GCTCGGTGTG CCGTGGCAAG 
GGAAAAATCT TAGTAGGAAC CAAAGATGGT GAAATAATTG AAGTTGGGGA AAAAAGCGCT 
GCTTCAAACA TCCTGATTGA TGGACACATG GAGGGGGAGA TCTGGGGCCT AGCCACACAC 
CCCTCCAAGG ACATGTTCAT CTCAGCCAGC AATGATGGCA CAGCAAGGAT CTGGGACCTG 
GCTGACAAGA AACTGTTAAA CAAGGTGAAC CTGGGCCATG CGGCCAGGTG TGCAGCCTAT 
AGCCCTGATG GGGAGATGGT GGCCATTGGC ATGAAGAATG GAGAGTTTGT CATCTTGCTG 
GTGAACACCC TGAAAGTTTG GGGGAAAAAA CGAGACCGGA AATCTGCCAT CCAAGATATC 
AGAATCAGCC CAGACAACAG ATTCTTAGCT GTGGGGTCTT CTGAGCAGAC AGTTGACTTC 
TATGACCTTA CTCAGGGCAC AAGTCTGAAC CGGATCGGCT ACTGCAAAGA CATCCCAAGC 
TTTGTCATTC AGATGGACTT TTCTGCAGAC AGCAAATACA TTCAGGTGTC AACAGGAGCC 
TATAAGCGCC AAGTACATGA AGTTCCCCTG GGGAAGCAAG TAACTGAAGC CATGGTTGTT 
GAGAAGATCA CCTGGGCTTC CTGGACAAGT GTTCTCGGAG ATGAAGTCAT TGGAATCTGG 
CCACGAAACG CAGACAAGGC TGATGTCAAC TGTGCATGTG TGACCCATGC TGGCTTGAAC 
ATTGTCACAG GGGATGACTT CGGGCTGCTG AAGCTCTTTG ATTTTCCATG CACAGAAAAA 
TTCGCCAAGC ACAAACGTTA CTTTGGTCAC TCTGCTCATG TGACGAACAT CCGCTTCTCT 
TCCGATGACA AGTATGTGGT CAGCACAGGA GGAGACGACT GCAGTGTATT TGTGTGGCGA 
TGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_017314515.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170004.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X4, mRNA.

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CloneID	OMu77889
Clone ID Related Accession (Same CDS sequence)	XM_017314515.1 , XM_017314515.2
Accession Version	XM_017314515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314515.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGGGATTC GTGTGGAGAA AAAGACAAGT AGGTTGACAT CAAATGTGGG ACTACAAGAG 
ATTTGGAAGA ACAGATCTGA CGGAAAATGT CTCGTGTCGG TTGGTTTAGA CGATTTTCAC 
AGTGTTGTGT TTTGGGACTG GAAAAAGGGA GAAAAGATAG CCACAACCAG GGGACACAAA 
GACAAAATAT TTGTGGTAAA GTGTAATCCA CAGCATGCTG ACAAGTTGGT GACAGTTGGG 
ATTAAGCATA TCAAGTTCTG GCAACAAGCA GGTGGGGGCT TCACCTCTAA AAGAGGAAGT 
TTTGGAAGTG CTGGAAAGTT GGAAACAATG ATGTGTGTTT CTTATGGGCG AATGGAAGAT 
CTGGTGTTCT CAGGTGCAGC CACTGGAGAT ATTTTTATCT GGAAGGACGT TCTGCTACTG 
AAGACAGTGA AGGCTCATGA TGGGCCTGTG TTTGCCATGT ATGCATTAGA CAAGGGCTTT 
GTAACAGGTG GAAAGGATGG AATTGTGGAA CTCTGGGATG ATATGTTTGA AAGATGCTTG 
AAGACTTATG CCATCAAAAG GACTGCATTG TCAACCAGCT CAAAAGGACT GCTTTTGGAG 
GATAACCCTT CAATTCGTGC AATTACCCTA GGGCATGGAC ACATTCTCGT GGGGACAAAA 
AATGGAGAGA TTCTAGAAAT TGATAAGAGT GGCCCGATGA CCCTGCTTGT TCAGGGACAC 
ATGGAAGGGG AAGTGTGGGG GCTGGCAGCA CACCCGCTCC TGCCCATCTG CGCGACAGTA 
AGTGATGACA AAACACTTCG GATCTGGGAA TTATCCTCCC AGCACCGCAT GCTGGCTGTA 
CGGAAACTTA AAAAAGGAGG AAGATGTTGT GCTTTCTCCC CTGATGGGAA AGCCCTAGCA 
GTTGGATTGA ATGATGGAAG TTTCTTGGTG GTGAATGCTG ACACAGTGGA AGACATGCTC 
TCCTTCCATC ACAGAAAAGA AATGATCTCT GATATAAAAT TTTCAAAAGA TACAGGAAAA 
TACCTTGCTG TGGCATCTCA TGATAACTTC GTGGATATCT ACAACGTGCT TACAAGCAAG 
AGGGTTGGCA TCTGTAAAGG AGCTTCTAGT TACATCACAC ACATTGACTG GGACTCTAGA 
GGGAAATTAC TGCAAGTTAA TTCAGGTGCC AAAGAACAGC TTTTCTTTGA AGCTCCAAGA 
GGCAGAAAAC ACACAATAAG GCCATCAGAG GCTGAGAAGA TAGAATGGGA CACCTGGACC 
TGTGTCCTGG GGCCCACCTG TGAAGGAATC TGGCCTGCTC ACAGCGATGT GACTGACGTC 
AATGCTGCCA ATCTTACCAA AGATGGCTCC CTCTTAGCAA CTGGGGATGA CTTTGGTTTT 
GTGAAGCTTT TTTCATATCC TGTCAAGGGA CAGCATGCAA GATTTAAGAA ATATGTGGGG 
CACAGCGCAC ACGTTACCAA TGTGAGGTGG CTACACAATG ACTCTGTGCT GCTCACTGTA 
GGAGGTGCTG ACACAGCCTT GATGATCTGG ACCAGGGAAT TTGTGGGGAC GCAGGAGAGC 
AAGCTGGTTG ATAGTGAGGA GTCAGACACA GATGCAGAAG AAGATGGAGG CTATGACAGT 
GATGTCGCTA GAGAGAAAGC CATTGACTAT ACCACCAAGA TCTATGCTGT GAGCATCAGG 
GAAATGGAAG GCACCAAACC ACACCAGCAG CTAAAAGAGG TGTCCATGGA AGAAAGACCA 
CCCGTTAGCC GTGCAGCCCC CCAGCCCGAG AAACTCCAGA AGAACAACAT CACCAAGAAA 
AAGAAGCTGG TTGAGGAGCT GGCTCTGGAC CACGTGTTTG GCTACCGAGG ATTTGACTGC 
CGCAACAACC TGCATTACCT TAACGATGGT GCCGACATCA TTTTCCACAC TGCGGCAGCT 
GGCATTGTGC AGAACCTCTC CACAGGCAGC CAGAGCTTCT ACCTGGAGCA CACGGATGAC 
ATCCTCTGCC TCACAGTGAA TCAGCACCCA AAGTACAGAA ATGTGGTGGC TACCAGCCAG 
ATAGGGACAA CACCTTCAAT TCATATCTGG GATGCAATGA CCAAACACAC CCTTTCCATG 
CTGCGGTGCT TCCACACCAA GGGGGTGAAT TACATCAACT TCAGTGCAAC CGGGAAGCTC 
CTGGTGTCTG TGGGCGTGGA TCCAGAGCAC ACCATCACTG TCTGGAGATG GCAAGAAGGT 
ACCAAGGTTG CCAGCCGAGG GGGCCACCTG GAACGAATAT TTGTGGTGGA ATTTCGCCCT 
GACTCAGACA CACAATTTGT ATCTGTTGGA GTCAAACATA TGAAGTTCTG GACTCTGGCA 
GGAAGTGCTC TGCTGTACAA GAAAGGGGTC ATCGGGTCTA TGGAAGCTGC CAAGATGCAA 
ACGATGCTGT CCGTGGCTTT TGGTGCTAAC AACCTTACTT TTACTGGTGC CATCAATGGA 
GATGTCTATG TCTGGAAGGA GCACTTCCTC ATCCGGCTGG TGGCCAAGGC TCACACAGGT 
CCTGTCTTTA CAATGTACAC CACCCTCCGA GATGGGCTTA TTGTGACTGG CGGAAAAGAA 
CGACCGACTA AGGAAGGAGG AGCTGTAAAG TTGTGGGACC AGGAGATGAA GCGCTGTCGG 
GCCTTCCAGC TGGAAACAGG GCAGCTGGTG GAATGTGTGC GCTCGGTGTG CCGTGGCAAG 
GGAAAAATCT TAGTAGGAAC CAAAGATGGT GAAATAATTG AAGTTGGGGA AAAAAGCGCT 
GCTTCAAACA TCCTGATTGA TGGACACATG GAGGGGGAGA TCTGGGGCCT AGCCACACAC 
CCCTCCAAGG ACATGTTCAT CTCAGCCAGC AATGATGGCA CAGCAAGGAT CTGGGACCTG 
GCTGACAAGA AACTGTTAAA CAAGGTGAAC CTGGGCCATG CGGCCAGGTG TGCAGCCTAT 
AGCCCTGATG GGGAGATGGT GGCCATTGGC ATGAAGAATG GAGAGTTTGT CATCTTGCTG 
GTGAACACCC TGAAAGTTTG GGGGAAAAAA CGAGACCGGA AATCTGCCAT CCAAGATATC 
AGAATCAGCC CAGACAACAG ATTCTTAGCT GTGGGGTCTT CTGAGCAGAC AGTTGACTTC 
TATGACCTTA CTCAGGGCAC AAGTCTGAAC CGGATCGGCT ACTGCAAAGA CATCCCAAGC 
TTTGTCATTC AGATGGACTT TTCTGCAGAC AGCAAATACA TTCAGGTGTC AACAGGAGCC 
TATAAGCGCC AAGTACATGA AGTTCCCCTG GGGAAGCAAG TAACTGAAGC CATGGTTGTT 
GAGAAGATCA CCTGGGCTTC CTGGACAAGT GTTCTCGGAG ATGAAGTCAT TGGAATCTGG 
CCACGAAACG CAGACAAGGC TGATGTCAAC TGTGCATGTG TGACCCATGC TGGCTTGAAC 
ATTGTCACAG GGGATGACTT CGGGCTGCTG AAGCTCTTTG ATTTTCCATG CACAGAAAAA 
TTCGCCAAGC ACAAACGTTA CTTTGGTCAC TCTGCTCATG TGACGAACAT CCGCTTCTCT 
TCCGATGACA AGTATGTGGT CAGCACAGGA GGAGACGACT GCAGTGTATT TGTGTGGCGA 
TGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170004.1
CDS	681..6629
Translation	MADRTAPRCQLRLEWVYGYRGHQCRNNLYYTAGKEVVYFVAGVGVVYNTREHSQKFFLGHNDDIISLALHPDKTLIATGQVGKEPYICIWNSYNVHTVSILKDVHTHGVACLAFDSDGQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPSGKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSGIRVEKKTSRLTSNVGLQEIWKNRSDGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_017314515.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314515.2

ORF Insert Sequence:

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGGGATTC GTGTGGAGAA AAAGACAAGT AGGTTGACAT CAAATGTGGG ACTACAAGAG 
ATTTGGAAGA ACAGATCTGA CGGAAAATGT CTCGTGTCGG TTGGTTTAGA CGATTTTCAC 
AGTGTTGTGT TTTGGGACTG GAAAAAGGGA GAAAAGATAG CCACAACCAG GGGACACAAA 
GACAAAATAT TTGTGGTAAA GTGTAATCCA CAGCATGCTG ACAAGTTGGT GACAGTTGGG 
ATTAAGCATA TCAAGTTCTG GCAACAAGCA GGTGGGGGCT TCACCTCTAA AAGAGGAAGT 
TTTGGAAGTG CTGGAAAGTT GGAAACAATG ATGTGTGTTT CTTATGGGCG AATGGAAGAT 
CTGGTGTTCT CAGGTGCAGC CACTGGAGAT ATTTTTATCT GGAAGGACGT TCTGCTACTG 
AAGACAGTGA AGGCTCATGA TGGGCCTGTG TTTGCCATGT ATGCATTAGA CAAGGGCTTT 
GTAACAGGTG GAAAGGATGG AATTGTGGAA CTCTGGGATG ATATGTTTGA AAGATGCTTG 
AAGACTTATG CCATCAAAAG GACTGCATTG TCAACCAGCT CAAAAGGACT GCTTTTGGAG 
GATAACCCTT CAATTCGTGC AATTACCCTA GGGCATGGAC ACATTCTCGT GGGGACAAAA 
AATGGAGAGA TTCTAGAAAT TGATAAGAGT GGCCCGATGA CCCTGCTTGT TCAGGGACAC 
ATGGAAGGGG AAGTGTGGGG GCTGGCAGCA CACCCGCTCC TGCCCATCTG CGCGACAGTA 
AGTGATGACA AAACACTTCG GATCTGGGAA TTATCCTCCC AGCACCGCAT GCTGGCTGTA 
CGGAAACTTA AAAAAGGAGG AAGATGTTGT GCTTTCTCCC CTGATGGGAA AGCCCTAGCA 
GTTGGATTGA ATGATGGAAG TTTCTTGGTG GTGAATGCTG ACACAGTGGA AGACATGCTC 
TCCTTCCATC ACAGAAAAGA AATGATCTCT GATATAAAAT TTTCAAAAGA TACAGGAAAA 
TACCTTGCTG TGGCATCTCA TGATAACTTC GTGGATATCT ACAACGTGCT TACAAGCAAG 
AGGGTTGGCA TCTGTAAAGG AGCTTCTAGT TACATCACAC ACATTGACTG GGACTCTAGA 
GGGAAATTAC TGCAAGTTAA TTCAGGTGCC AAAGAACAGC TTTTCTTTGA AGCTCCAAGA 
GGCAGAAAAC ACACAATAAG GCCATCAGAG GCTGAGAAGA TAGAATGGGA CACCTGGACC 
TGTGTCCTGG GGCCCACCTG TGAAGGAATC TGGCCTGCTC ACAGCGATGT GACTGACGTC 
AATGCTGCCA ATCTTACCAA AGATGGCTCC CTCTTAGCAA CTGGGGATGA CTTTGGTTTT 
GTGAAGCTTT TTTCATATCC TGTCAAGGGA CAGCATGCAA GATTTAAGAA ATATGTGGGG 
CACAGCGCAC ACGTTACCAA TGTGAGGTGG CTACACAATG ACTCTGTGCT GCTCACTGTA 
GGAGGTGCTG ACACAGCCTT GATGATCTGG ACCAGGGAAT TTGTGGGGAC GCAGGAGAGC 
AAGCTGGTTG ATAGTGAGGA GTCAGACACA GATGCAGAAG AAGATGGAGG CTATGACAGT 
GATGTCGCTA GAGAGAAAGC CATTGACTAT ACCACCAAGA TCTATGCTGT GAGCATCAGG 
GAAATGGAAG GCACCAAACC ACACCAGCAG CTAAAAGAGG TGTCCATGGA AGAAAGACCA 
CCCGTTAGCC GTGCAGCCCC CCAGCCCGAG AAACTCCAGA AGAACAACAT CACCAAGAAA 
AAGAAGCTGG TTGAGGAGCT GGCTCTGGAC CACGTGTTTG GCTACCGAGG ATTTGACTGC 
CGCAACAACC TGCATTACCT TAACGATGGT GCCGACATCA TTTTCCACAC TGCGGCAGCT 
GGCATTGTGC AGAACCTCTC CACAGGCAGC CAGAGCTTCT ACCTGGAGCA CACGGATGAC 
ATCCTCTGCC TCACAGTGAA TCAGCACCCA AAGTACAGAA ATGTGGTGGC TACCAGCCAG 
ATAGGGACAA CACCTTCAAT TCATATCTGG GATGCAATGA CCAAACACAC CCTTTCCATG 
CTGCGGTGCT TCCACACCAA GGGGGTGAAT TACATCAACT TCAGTGCAAC CGGGAAGCTC 
CTGGTGTCTG TGGGCGTGGA TCCAGAGCAC ACCATCACTG TCTGGAGATG GCAAGAAGGT 
ACCAAGGTTG CCAGCCGAGG GGGCCACCTG GAACGAATAT TTGTGGTGGA ATTTCGCCCT 
GACTCAGACA CACAATTTGT ATCTGTTGGA GTCAAACATA TGAAGTTCTG GACTCTGGCA 
GGAAGTGCTC TGCTGTACAA GAAAGGGGTC ATCGGGTCTA TGGAAGCTGC CAAGATGCAA 
ACGATGCTGT CCGTGGCTTT TGGTGCTAAC AACCTTACTT TTACTGGTGC CATCAATGGA 
GATGTCTATG TCTGGAAGGA GCACTTCCTC ATCCGGCTGG TGGCCAAGGC TCACACAGGT 
CCTGTCTTTA CAATGTACAC CACCCTCCGA GATGGGCTTA TTGTGACTGG CGGAAAAGAA 
CGACCGACTA AGGAAGGAGG AGCTGTAAAG TTGTGGGACC AGGAGATGAA GCGCTGTCGG 
GCCTTCCAGC TGGAAACAGG GCAGCTGGTG GAATGTGTGC GCTCGGTGTG CCGTGGCAAG 
GGAAAAATCT TAGTAGGAAC CAAAGATGGT GAAATAATTG AAGTTGGGGA AAAAAGCGCT 
GCTTCAAACA TCCTGATTGA TGGACACATG GAGGGGGAGA TCTGGGGCCT AGCCACACAC 
CCCTCCAAGG ACATGTTCAT CTCAGCCAGC AATGATGGCA CAGCAAGGAT CTGGGACCTG 
GCTGACAAGA AACTGTTAAA CAAGGTGAAC CTGGGCCATG CGGCCAGGTG TGCAGCCTAT 
AGCCCTGATG GGGAGATGGT GGCCATTGGC ATGAAGAATG GAGAGTTTGT CATCTTGCTG 
GTGAACACCC TGAAAGTTTG GGGGAAAAAA CGAGACCGGA AATCTGCCAT CCAAGATATC 
AGAATCAGCC CAGACAACAG ATTCTTAGCT GTGGGGTCTT CTGAGCAGAC AGTTGACTTC 
TATGACCTTA CTCAGGGCAC AAGTCTGAAC CGGATCGGCT ACTGCAAAGA CATCCCAAGC 
TTTGTCATTC AGATGGACTT TTCTGCAGAC AGCAAATACA TTCAGGTGTC AACAGGAGCC 
TATAAGCGCC AAGTACATGA AGTTCCCCTG GGGAAGCAAG TAACTGAAGC CATGGTTGTT 
GAGAAGATCA CCTGGGCTTC CTGGACAAGT GTTCTCGGAG ATGAAGTCAT TGGAATCTGG 
CCACGAAACG CAGACAAGGC TGATGTCAAC TGTGCATGTG TGACCCATGC TGGCTTGAAC 
ATTGTCACAG GGGATGACTT CGGGCTGCTG AAGCTCTTTG ATTTTCCATG CACAGAAAAA 
TTCGCCAAGC ACAAACGTTA CTTTGGTCAC TCTGCTCATG TGACGAACAT CCGCTTCTCT 
TCCGATGACA AGTATGTGGT CAGCACAGGA GGAGACGACT GCAGTGTATT TGTGTGGCGA 
TGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_017314516.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170005.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X5, mRNA.

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CloneID	OMu77890
Clone ID Related Accession (Same CDS sequence)	XM_017314516.1 , XM_017314516.2
Accession Version	XM_017314516.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5901bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGCTGACG GAAAATGTCT CGTGTCGGTT GGTTTAGACG ATTTTCACAG TGTTGTGTTT 
TGGGACTGGA AAAAGGGAGA AAAGATAGCC ACAACCAGGG GACACAAAGA CAAAATATTT 
GTGGTAAAGT GTAATCCACA GCATGCTGAC AAGTTGGTGA CAGTTGGGAT TAAGCATATC 
AAGTTCTGGC AACAAGCAGG TGGGGGCTTC ACCTCTAAAA GAGGAAGTTT TGGAAGTGCT 
GGAAAGTTGG AAACAATGAT GTGTGTTTCT TATGGGCGAA TGGAAGATCT GGTGTTCTCA 
GGTGCAGCCA CTGGAGATAT TTTTATCTGG AAGGACGTTC TGCTACTGAA GACAGTGAAG 
GCTCATGATG GGCCTGTGTT TGCCATGTAT GCATTAGACA AGGGCTTTGT AACAGGTGGA 
AAGGATGGAA TTGTGGAACT CTGGGATGAT ATGTTTGAAA GATGCTTGAA GACTTATGCC 
ATCAAAAGGA CTGCATTGTC AACCAGCTCA AAAGGACTGC TTTTGGAGGA TAACCCTTCA 
ATTCGTGCAA TTACCCTAGG GCATGGACAC ATTCTCGTGG GGACAAAAAA TGGAGAGATT 
CTAGAAATTG ATAAGAGTGG CCCGATGACC CTGCTTGTTC AGGGACACAT GGAAGGGGAA 
GTGTGGGGGC TGGCAGCACA CCCGCTCCTG CCCATCTGCG CGACAGTAAG TGATGACAAA 
ACACTTCGGA TCTGGGAATT ATCCTCCCAG CACCGCATGC TGGCTGTACG GAAACTTAAA 
AAAGGAGGAA GATGTTGTGC TTTCTCCCCT GATGGGAAAG CCCTAGCAGT TGGATTGAAT 
GATGGAAGTT TCTTGGTGGT GAATGCTGAC ACAGTGGAAG ACATGCTCTC CTTCCATCAC 
AGAAAAGAAA TGATCTCTGA TATAAAATTT TCAAAAGATA CAGGAAAATA CCTTGCTGTG 
GCATCTCATG ATAACTTCGT GGATATCTAC AACGTGCTTA CAAGCAAGAG GGTTGGCATC 
TGTAAAGGAG CTTCTAGTTA CATCACACAC ATTGACTGGG ACTCTAGAGG GAAATTACTG 
CAAGTTAATT CAGGTGCCAA AGAACAGCTT TTCTTTGAAG CTCCAAGAGG CAGAAAACAC 
ACAATAAGGC CATCAGAGGC TGAGAAGATA GAATGGGACA CCTGGACCTG TGTCCTGGGG 
CCCACCTGTG AAGGAATCTG GCCTGCTCAC AGCGATGTGA CTGACGTCAA TGCTGCCAAT 
CTTACCAAAG ATGGCTCCCT CTTAGCAACT GGGGATGACT TTGGTTTTGT GAAGCTTTTT 
TCATATCCTG TCAAGGGACA GCATGCAAGA TTTAAGAAAT ATGTGGGGCA CAGCGCACAC 
GTTACCAATG TGAGGTGGCT ACACAATGAC TCTGTGCTGC TCACTGTAGG AGGTGCTGAC 
ACAGCCTTGA TGATCTGGAC CAGGGAATTT GTGGGGACGC AGGAGAGCAA GCTGGTTGAT 
AGTGAGGAGT CAGACACAGA TGCAGAAGAA GATGGAGGCT ATGACAGTGA TGTCGCTAGA 
GAGAAAGCCA TTGACTATAC CACCAAGATC TATGCTGTGA GCATCAGGGA AATGGAAGGC 
ACCAAACCAC ACCAGCAGCT AAAAGAGGTG TCCATGGAAG AAAGGCAGGG AATTGTCAAG 
GGATCCAGAC CACCCGTTAG CCGTGCAGCC CCCCAGCCCG AGAAACTCCA GAAGAACAAC 
ATCACCAAGA AAAAGAAGCT GGTTGAGGAG CTGGCTCTGG ACCACGTGTT TGGCTACCGA 
GGATTTGACT GCCGCAACAA CCTGCATTAC CTTAACGATG GTGCCGACAT CATTTTCCAC 
ACTGCGGCAG CTGGCATTGT GCAGAACCTC TCCACAGGCA GCCAGAGCTT CTACCTGGAG 
CACACGGATG ACATCCTCTG CCTCACAGTG AATCAGCACC CAAAGTACAG AAATGTGGTG 
GCTACCAGCC AGATAGGGAC AACACCTTCA ATTCATATCT GGGATGCAAT GACCAAACAC 
ACCCTTTCCA TGCTGCGGTG CTTCCACACC AAGGGGGTGA ATTACATCAA CTTCAGTGCA 
ACCGGGAAGC TCCTGGTGTC TGTGGGCGTG GATCCAGAGC ACACCATCAC TGTCTGGAGA 
TGGCAAGAAG GTACCAAGGT TGCCAGCCGA GGGGGCCACC TGGAACGAAT ATTTGTGGTG 
GAATTTCGCC CTGACTCAGA CACACAATTT GTATCTGTTG GAGTCAAACA TATGAAGTTC 
TGGACTCTGG CAGGAAGTGC TCTGCTGTAC AAGAAAGGGG TCATCGGGTC TATGGAAGCT 
GCCAAGATGC AAACGATGCT GTCCGTGGCT TTTGGTGCTA ACAACCTTAC TTTTACTGGT 
GCCATCAATG GAGATGTCTA TGTCTGGAAG GAGCACTTCC TCATCCGGCT GGTGGCCAAG 
GCTCACACAG GTCCTGTCTT TACAATGTAC ACCACCCTCC GAGATGGGCT TATTGTGACT 
GGCGGAAAAG AACGACCGAC TAAGGAAGGA GGAGCTGTAA AGTTGTGGGA CCAGGAGATG 
AAGCGCTGTC GGGCCTTCCA GCTGGAAACA GGGCAGCTGG TGGAATGTGT GCGCTCGGTG 
TGCCGTGGCA AGGGAAAAAT CTTAGTAGGA ACCAAAGATG GTGAAATAAT TGAAGTTGGG 
GAAAAAAGCG CTGCTTCAAA CATCCTGATT GATGGACACA TGGAGGGGGA GATCTGGGGC 
CTAGCCACAC ACCCCTCCAA GGACATGTTC ATCTCAGCCA GCAATGATGG CACAGCAAGG 
ATCTGGGACC TGGCTGACAA GAAACTGTTA AACAAGGTGA ACCTGGGCCA TGCGGCCAGG 
TGTGCAGCCT ATAGCCCTGA TGGGGAGATG GTGGCCATTG GCATGAAGAA TGGAGAGTTT 
GTCATCTTGC TGGTGAACAC CCTGAAAGTT TGGGGGAAAA AACGAGACCG GAAATCTGCC 
ATCCAAGATA TCAGAATCAG CCCAGACAAC AGATTCTTAG CTGTGGGGTC TTCTGAGCAG 
ACAGTTGACT TCTATGACCT TACTCAGGGC ACAAGTCTGA ACCGGATCGG CTACTGCAAA 
GACATCCCAA GCTTTGTCAT TCAGATGGAC TTTTCTGCAG ACAGCAAATA CATTCAGGTG 
TCAACAGGAG CCTATAAGCG CCAAGTACAT GAAGTTCCCC TGGGGAAGCA AGTAACTGAA 
GCCATGGTTG TTGAGAAGAT CACCTGGGCT TCCTGGACAA GTGTTCTCGG AGATGAAGTC 
ATTGGAATCT GGCCACGAAA CGCAGACAAG GCTGATGTCA ACTGTGCATG TGTGACCCAT 
GCTGGCTTGA ACATTGTCAC AGGGGATGAC TTCGGGCTGC TGAAGCTCTT TGATTTTCCA 
TGCACAGAAA AATTCGCCAA GCACAAACGT TACTTTGGTC ACTCTGCTCA TGTGACGAAC 
ATCCGCTTCT CTTCCGATGA CAAGTATGTG GTCAGCACAG GAGGAGACGA CTGCAGTGTA 
TTTGTGTGGC GATGTCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170005.1
CDS	670..6570
Translation	MADRTAPRCQLRLEWVYGYRGHQCRNNLYYTAGKEVVYFVAGVGVVYNTREHSQKFFLGHNDDIISLALHPDKTLIATGQVGKEPYICIWNSYNVHTVSILKDVHTHGVACLAFDSDGQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPSGKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSADGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERQGIVKGSRPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_017314516.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314516.1

ORF Insert Sequence:

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGCTGACG GAAAATGTCT CGTGTCGGTT GGTTTAGACG ATTTTCACAG TGTTGTGTTT 
TGGGACTGGA AAAAGGGAGA AAAGATAGCC ACAACCAGGG GACACAAAGA CAAAATATTT 
GTGGTAAAGT GTAATCCACA GCATGCTGAC AAGTTGGTGA CAGTTGGGAT TAAGCATATC 
AAGTTCTGGC AACAAGCAGG TGGGGGCTTC ACCTCTAAAA GAGGAAGTTT TGGAAGTGCT 
GGAAAGTTGG AAACAATGAT GTGTGTTTCT TATGGGCGAA TGGAAGATCT GGTGTTCTCA 
GGTGCAGCCA CTGGAGATAT TTTTATCTGG AAGGACGTTC TGCTACTGAA GACAGTGAAG 
GCTCATGATG GGCCTGTGTT TGCCATGTAT GCATTAGACA AGGGCTTTGT AACAGGTGGA 
AAGGATGGAA TTGTGGAACT CTGGGATGAT ATGTTTGAAA GATGCTTGAA GACTTATGCC 
ATCAAAAGGA CTGCATTGTC AACCAGCTCA AAAGGACTGC TTTTGGAGGA TAACCCTTCA 
ATTCGTGCAA TTACCCTAGG GCATGGACAC ATTCTCGTGG GGACAAAAAA TGGAGAGATT 
CTAGAAATTG ATAAGAGTGG CCCGATGACC CTGCTTGTTC AGGGACACAT GGAAGGGGAA 
GTGTGGGGGC TGGCAGCACA CCCGCTCCTG CCCATCTGCG CGACAGTAAG TGATGACAAA 
ACACTTCGGA TCTGGGAATT ATCCTCCCAG CACCGCATGC TGGCTGTACG GAAACTTAAA 
AAAGGAGGAA GATGTTGTGC TTTCTCCCCT GATGGGAAAG CCCTAGCAGT TGGATTGAAT 
GATGGAAGTT TCTTGGTGGT GAATGCTGAC ACAGTGGAAG ACATGCTCTC CTTCCATCAC 
AGAAAAGAAA TGATCTCTGA TATAAAATTT TCAAAAGATA CAGGAAAATA CCTTGCTGTG 
GCATCTCATG ATAACTTCGT GGATATCTAC AACGTGCTTA CAAGCAAGAG GGTTGGCATC 
TGTAAAGGAG CTTCTAGTTA CATCACACAC ATTGACTGGG ACTCTAGAGG GAAATTACTG 
CAAGTTAATT CAGGTGCCAA AGAACAGCTT TTCTTTGAAG CTCCAAGAGG CAGAAAACAC 
ACAATAAGGC CATCAGAGGC TGAGAAGATA GAATGGGACA CCTGGACCTG TGTCCTGGGG 
CCCACCTGTG AAGGAATCTG GCCTGCTCAC AGCGATGTGA CTGACGTCAA TGCTGCCAAT 
CTTACCAAAG ATGGCTCCCT CTTAGCAACT GGGGATGACT TTGGTTTTGT GAAGCTTTTT 
TCATATCCTG TCAAGGGACA GCATGCAAGA TTTAAGAAAT ATGTGGGGCA CAGCGCACAC 
GTTACCAATG TGAGGTGGCT ACACAATGAC TCTGTGCTGC TCACTGTAGG AGGTGCTGAC 
ACAGCCTTGA TGATCTGGAC CAGGGAATTT GTGGGGACGC AGGAGAGCAA GCTGGTTGAT 
AGTGAGGAGT CAGACACAGA TGCAGAAGAA GATGGAGGCT ATGACAGTGA TGTCGCTAGA 
GAGAAAGCCA TTGACTATAC CACCAAGATC TATGCTGTGA GCATCAGGGA AATGGAAGGC 
ACCAAACCAC ACCAGCAGCT AAAAGAGGTG TCCATGGAAG AAAGGCAGGG AATTGTCAAG 
GGATCCAGAC CACCCGTTAG CCGTGCAGCC CCCCAGCCCG AGAAACTCCA GAAGAACAAC 
ATCACCAAGA AAAAGAAGCT GGTTGAGGAG CTGGCTCTGG ACCACGTGTT TGGCTACCGA 
GGATTTGACT GCCGCAACAA CCTGCATTAC CTTAACGATG GTGCCGACAT CATTTTCCAC 
ACTGCGGCAG CTGGCATTGT GCAGAACCTC TCCACAGGCA GCCAGAGCTT CTACCTGGAG 
CACACGGATG ACATCCTCTG CCTCACAGTG AATCAGCACC CAAAGTACAG AAATGTGGTG 
GCTACCAGCC AGATAGGGAC AACACCTTCA ATTCATATCT GGGATGCAAT GACCAAACAC 
ACCCTTTCCA TGCTGCGGTG CTTCCACACC AAGGGGGTGA ATTACATCAA CTTCAGTGCA 
ACCGGGAAGC TCCTGGTGTC TGTGGGCGTG GATCCAGAGC ACACCATCAC TGTCTGGAGA 
TGGCAAGAAG GTACCAAGGT TGCCAGCCGA GGGGGCCACC TGGAACGAAT ATTTGTGGTG 
GAATTTCGCC CTGACTCAGA CACACAATTT GTATCTGTTG GAGTCAAACA TATGAAGTTC 
TGGACTCTGG CAGGAAGTGC TCTGCTGTAC AAGAAAGGGG TCATCGGGTC TATGGAAGCT 
GCCAAGATGC AAACGATGCT GTCCGTGGCT TTTGGTGCTA ACAACCTTAC TTTTACTGGT 
GCCATCAATG GAGATGTCTA TGTCTGGAAG GAGCACTTCC TCATCCGGCT GGTGGCCAAG 
GCTCACACAG GTCCTGTCTT TACAATGTAC ACCACCCTCC GAGATGGGCT TATTGTGACT 
GGCGGAAAAG AACGACCGAC TAAGGAAGGA GGAGCTGTAA AGTTGTGGGA CCAGGAGATG 
AAGCGCTGTC GGGCCTTCCA GCTGGAAACA GGGCAGCTGG TGGAATGTGT GCGCTCGGTG 
TGCCGTGGCA AGGGAAAAAT CTTAGTAGGA ACCAAAGATG GTGAAATAAT TGAAGTTGGG 
GAAAAAAGCG CTGCTTCAAA CATCCTGATT GATGGACACA TGGAGGGGGA GATCTGGGGC 
CTAGCCACAC ACCCCTCCAA GGACATGTTC ATCTCAGCCA GCAATGATGG CACAGCAAGG 
ATCTGGGACC TGGCTGACAA GAAACTGTTA AACAAGGTGA ACCTGGGCCA TGCGGCCAGG 
TGTGCAGCCT ATAGCCCTGA TGGGGAGATG GTGGCCATTG GCATGAAGAA TGGAGAGTTT 
GTCATCTTGC TGGTGAACAC CCTGAAAGTT TGGGGGAAAA AACGAGACCG GAAATCTGCC 
ATCCAAGATA TCAGAATCAG CCCAGACAAC AGATTCTTAG CTGTGGGGTC TTCTGAGCAG 
ACAGTTGACT TCTATGACCT TACTCAGGGC ACAAGTCTGA ACCGGATCGG CTACTGCAAA 
GACATCCCAA GCTTTGTCAT TCAGATGGAC TTTTCTGCAG ACAGCAAATA CATTCAGGTG 
TCAACAGGAG CCTATAAGCG CCAAGTACAT GAAGTTCCCC TGGGGAAGCA AGTAACTGAA 
GCCATGGTTG TTGAGAAGAT CACCTGGGCT TCCTGGACAA GTGTTCTCGG AGATGAAGTC 
ATTGGAATCT GGCCACGAAA CGCAGACAAG GCTGATGTCA ACTGTGCATG TGTGACCCAT 
GCTGGCTTGA ACATTGTCAC AGGGGATGAC TTCGGGCTGC TGAAGCTCTT TGATTTTCCA 
TGCACAGAAA AATTCGCCAA GCACAAACGT TACTTTGGTC ACTCTGCTCA TGTGACGAAC 
ATCCGCTTCT CTTCCGATGA CAAGTATGTG GTCAGCACAG GAGGAGACGA CTGCAGTGTA 
TTTGTGTGGC GATGTCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_017314516.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170005.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X5, mRNA.

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CloneID	OMu77890
Clone ID Related Accession (Same CDS sequence)	XM_017314516.1 , XM_017314516.2
Accession Version	XM_017314516.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5901bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314516.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGCTGACG GAAAATGTCT CGTGTCGGTT GGTTTAGACG ATTTTCACAG TGTTGTGTTT 
TGGGACTGGA AAAAGGGAGA AAAGATAGCC ACAACCAGGG GACACAAAGA CAAAATATTT 
GTGGTAAAGT GTAATCCACA GCATGCTGAC AAGTTGGTGA CAGTTGGGAT TAAGCATATC 
AAGTTCTGGC AACAAGCAGG TGGGGGCTTC ACCTCTAAAA GAGGAAGTTT TGGAAGTGCT 
GGAAAGTTGG AAACAATGAT GTGTGTTTCT TATGGGCGAA TGGAAGATCT GGTGTTCTCA 
GGTGCAGCCA CTGGAGATAT TTTTATCTGG AAGGACGTTC TGCTACTGAA GACAGTGAAG 
GCTCATGATG GGCCTGTGTT TGCCATGTAT GCATTAGACA AGGGCTTTGT AACAGGTGGA 
AAGGATGGAA TTGTGGAACT CTGGGATGAT ATGTTTGAAA GATGCTTGAA GACTTATGCC 
ATCAAAAGGA CTGCATTGTC AACCAGCTCA AAAGGACTGC TTTTGGAGGA TAACCCTTCA 
ATTCGTGCAA TTACCCTAGG GCATGGACAC ATTCTCGTGG GGACAAAAAA TGGAGAGATT 
CTAGAAATTG ATAAGAGTGG CCCGATGACC CTGCTTGTTC AGGGACACAT GGAAGGGGAA 
GTGTGGGGGC TGGCAGCACA CCCGCTCCTG CCCATCTGCG CGACAGTAAG TGATGACAAA 
ACACTTCGGA TCTGGGAATT ATCCTCCCAG CACCGCATGC TGGCTGTACG GAAACTTAAA 
AAAGGAGGAA GATGTTGTGC TTTCTCCCCT GATGGGAAAG CCCTAGCAGT TGGATTGAAT 
GATGGAAGTT TCTTGGTGGT GAATGCTGAC ACAGTGGAAG ACATGCTCTC CTTCCATCAC 
AGAAAAGAAA TGATCTCTGA TATAAAATTT TCAAAAGATA CAGGAAAATA CCTTGCTGTG 
GCATCTCATG ATAACTTCGT GGATATCTAC AACGTGCTTA CAAGCAAGAG GGTTGGCATC 
TGTAAAGGAG CTTCTAGTTA CATCACACAC ATTGACTGGG ACTCTAGAGG GAAATTACTG 
CAAGTTAATT CAGGTGCCAA AGAACAGCTT TTCTTTGAAG CTCCAAGAGG CAGAAAACAC 
ACAATAAGGC CATCAGAGGC TGAGAAGATA GAATGGGACA CCTGGACCTG TGTCCTGGGG 
CCCACCTGTG AAGGAATCTG GCCTGCTCAC AGCGATGTGA CTGACGTCAA TGCTGCCAAT 
CTTACCAAAG ATGGCTCCCT CTTAGCAACT GGGGATGACT TTGGTTTTGT GAAGCTTTTT 
TCATATCCTG TCAAGGGACA GCATGCAAGA TTTAAGAAAT ATGTGGGGCA CAGCGCACAC 
GTTACCAATG TGAGGTGGCT ACACAATGAC TCTGTGCTGC TCACTGTAGG AGGTGCTGAC 
ACAGCCTTGA TGATCTGGAC CAGGGAATTT GTGGGGACGC AGGAGAGCAA GCTGGTTGAT 
AGTGAGGAGT CAGACACAGA TGCAGAAGAA GATGGAGGCT ATGACAGTGA TGTCGCTAGA 
GAGAAAGCCA TTGACTATAC CACCAAGATC TATGCTGTGA GCATCAGGGA AATGGAAGGC 
ACCAAACCAC ACCAGCAGCT AAAAGAGGTG TCCATGGAAG AAAGGCAGGG AATTGTCAAG 
GGATCCAGAC CACCCGTTAG CCGTGCAGCC CCCCAGCCCG AGAAACTCCA GAAGAACAAC 
ATCACCAAGA AAAAGAAGCT GGTTGAGGAG CTGGCTCTGG ACCACGTGTT TGGCTACCGA 
GGATTTGACT GCCGCAACAA CCTGCATTAC CTTAACGATG GTGCCGACAT CATTTTCCAC 
ACTGCGGCAG CTGGCATTGT GCAGAACCTC TCCACAGGCA GCCAGAGCTT CTACCTGGAG 
CACACGGATG ACATCCTCTG CCTCACAGTG AATCAGCACC CAAAGTACAG AAATGTGGTG 
GCTACCAGCC AGATAGGGAC AACACCTTCA ATTCATATCT GGGATGCAAT GACCAAACAC 
ACCCTTTCCA TGCTGCGGTG CTTCCACACC AAGGGGGTGA ATTACATCAA CTTCAGTGCA 
ACCGGGAAGC TCCTGGTGTC TGTGGGCGTG GATCCAGAGC ACACCATCAC TGTCTGGAGA 
TGGCAAGAAG GTACCAAGGT TGCCAGCCGA GGGGGCCACC TGGAACGAAT ATTTGTGGTG 
GAATTTCGCC CTGACTCAGA CACACAATTT GTATCTGTTG GAGTCAAACA TATGAAGTTC 
TGGACTCTGG CAGGAAGTGC TCTGCTGTAC AAGAAAGGGG TCATCGGGTC TATGGAAGCT 
GCCAAGATGC AAACGATGCT GTCCGTGGCT TTTGGTGCTA ACAACCTTAC TTTTACTGGT 
GCCATCAATG GAGATGTCTA TGTCTGGAAG GAGCACTTCC TCATCCGGCT GGTGGCCAAG 
GCTCACACAG GTCCTGTCTT TACAATGTAC ACCACCCTCC GAGATGGGCT TATTGTGACT 
GGCGGAAAAG AACGACCGAC TAAGGAAGGA GGAGCTGTAA AGTTGTGGGA CCAGGAGATG 
AAGCGCTGTC GGGCCTTCCA GCTGGAAACA GGGCAGCTGG TGGAATGTGT GCGCTCGGTG 
TGCCGTGGCA AGGGAAAAAT CTTAGTAGGA ACCAAAGATG GTGAAATAAT TGAAGTTGGG 
GAAAAAAGCG CTGCTTCAAA CATCCTGATT GATGGACACA TGGAGGGGGA GATCTGGGGC 
CTAGCCACAC ACCCCTCCAA GGACATGTTC ATCTCAGCCA GCAATGATGG CACAGCAAGG 
ATCTGGGACC TGGCTGACAA GAAACTGTTA AACAAGGTGA ACCTGGGCCA TGCGGCCAGG 
TGTGCAGCCT ATAGCCCTGA TGGGGAGATG GTGGCCATTG GCATGAAGAA TGGAGAGTTT 
GTCATCTTGC TGGTGAACAC CCTGAAAGTT TGGGGGAAAA AACGAGACCG GAAATCTGCC 
ATCCAAGATA TCAGAATCAG CCCAGACAAC AGATTCTTAG CTGTGGGGTC TTCTGAGCAG 
ACAGTTGACT TCTATGACCT TACTCAGGGC ACAAGTCTGA ACCGGATCGG CTACTGCAAA 
GACATCCCAA GCTTTGTCAT TCAGATGGAC TTTTCTGCAG ACAGCAAATA CATTCAGGTG 
TCAACAGGAG CCTATAAGCG CCAAGTACAT GAAGTTCCCC TGGGGAAGCA AGTAACTGAA 
GCCATGGTTG TTGAGAAGAT CACCTGGGCT TCCTGGACAA GTGTTCTCGG AGATGAAGTC 
ATTGGAATCT GGCCACGAAA CGCAGACAAG GCTGATGTCA ACTGTGCATG TGTGACCCAT 
GCTGGCTTGA ACATTGTCAC AGGGGATGAC TTCGGGCTGC TGAAGCTCTT TGATTTTCCA 
TGCACAGAAA AATTCGCCAA GCACAAACGT TACTTTGGTC ACTCTGCTCA TGTGACGAAC 
ATCCGCTTCT CTTCCGATGA CAAGTATGTG GTCAGCACAG GAGGAGACGA CTGCAGTGTA 
TTTGTGTGGC GATGTCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170005.1
CDS	681..6581
Translation	MADRTAPRCQLRLEWVYGYRGHQCRNNLYYTAGKEVVYFVAGVGVVYNTREHSQKFFLGHNDDIISLALHPDKTLIATGQVGKEPYICIWNSYNVHTVSILKDVHTHGVACLAFDSDGQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPSGKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSADGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERQGIVKGSRPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_017314516.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314516.2

ORF Insert Sequence:

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGCTGACG GAAAATGTCT CGTGTCGGTT GGTTTAGACG ATTTTCACAG TGTTGTGTTT 
TGGGACTGGA AAAAGGGAGA AAAGATAGCC ACAACCAGGG GACACAAAGA CAAAATATTT 
GTGGTAAAGT GTAATCCACA GCATGCTGAC AAGTTGGTGA CAGTTGGGAT TAAGCATATC 
AAGTTCTGGC AACAAGCAGG TGGGGGCTTC ACCTCTAAAA GAGGAAGTTT TGGAAGTGCT 
GGAAAGTTGG AAACAATGAT GTGTGTTTCT TATGGGCGAA TGGAAGATCT GGTGTTCTCA 
GGTGCAGCCA CTGGAGATAT TTTTATCTGG AAGGACGTTC TGCTACTGAA GACAGTGAAG 
GCTCATGATG GGCCTGTGTT TGCCATGTAT GCATTAGACA AGGGCTTTGT AACAGGTGGA 
AAGGATGGAA TTGTGGAACT CTGGGATGAT ATGTTTGAAA GATGCTTGAA GACTTATGCC 
ATCAAAAGGA CTGCATTGTC AACCAGCTCA AAAGGACTGC TTTTGGAGGA TAACCCTTCA 
ATTCGTGCAA TTACCCTAGG GCATGGACAC ATTCTCGTGG GGACAAAAAA TGGAGAGATT 
CTAGAAATTG ATAAGAGTGG CCCGATGACC CTGCTTGTTC AGGGACACAT GGAAGGGGAA 
GTGTGGGGGC TGGCAGCACA CCCGCTCCTG CCCATCTGCG CGACAGTAAG TGATGACAAA 
ACACTTCGGA TCTGGGAATT ATCCTCCCAG CACCGCATGC TGGCTGTACG GAAACTTAAA 
AAAGGAGGAA GATGTTGTGC TTTCTCCCCT GATGGGAAAG CCCTAGCAGT TGGATTGAAT 
GATGGAAGTT TCTTGGTGGT GAATGCTGAC ACAGTGGAAG ACATGCTCTC CTTCCATCAC 
AGAAAAGAAA TGATCTCTGA TATAAAATTT TCAAAAGATA CAGGAAAATA CCTTGCTGTG 
GCATCTCATG ATAACTTCGT GGATATCTAC AACGTGCTTA CAAGCAAGAG GGTTGGCATC 
TGTAAAGGAG CTTCTAGTTA CATCACACAC ATTGACTGGG ACTCTAGAGG GAAATTACTG 
CAAGTTAATT CAGGTGCCAA AGAACAGCTT TTCTTTGAAG CTCCAAGAGG CAGAAAACAC 
ACAATAAGGC CATCAGAGGC TGAGAAGATA GAATGGGACA CCTGGACCTG TGTCCTGGGG 
CCCACCTGTG AAGGAATCTG GCCTGCTCAC AGCGATGTGA CTGACGTCAA TGCTGCCAAT 
CTTACCAAAG ATGGCTCCCT CTTAGCAACT GGGGATGACT TTGGTTTTGT GAAGCTTTTT 
TCATATCCTG TCAAGGGACA GCATGCAAGA TTTAAGAAAT ATGTGGGGCA CAGCGCACAC 
GTTACCAATG TGAGGTGGCT ACACAATGAC TCTGTGCTGC TCACTGTAGG AGGTGCTGAC 
ACAGCCTTGA TGATCTGGAC CAGGGAATTT GTGGGGACGC AGGAGAGCAA GCTGGTTGAT 
AGTGAGGAGT CAGACACAGA TGCAGAAGAA GATGGAGGCT ATGACAGTGA TGTCGCTAGA 
GAGAAAGCCA TTGACTATAC CACCAAGATC TATGCTGTGA GCATCAGGGA AATGGAAGGC 
ACCAAACCAC ACCAGCAGCT AAAAGAGGTG TCCATGGAAG AAAGGCAGGG AATTGTCAAG 
GGATCCAGAC CACCCGTTAG CCGTGCAGCC CCCCAGCCCG AGAAACTCCA GAAGAACAAC 
ATCACCAAGA AAAAGAAGCT GGTTGAGGAG CTGGCTCTGG ACCACGTGTT TGGCTACCGA 
GGATTTGACT GCCGCAACAA CCTGCATTAC CTTAACGATG GTGCCGACAT CATTTTCCAC 
ACTGCGGCAG CTGGCATTGT GCAGAACCTC TCCACAGGCA GCCAGAGCTT CTACCTGGAG 
CACACGGATG ACATCCTCTG CCTCACAGTG AATCAGCACC CAAAGTACAG AAATGTGGTG 
GCTACCAGCC AGATAGGGAC AACACCTTCA ATTCATATCT GGGATGCAAT GACCAAACAC 
ACCCTTTCCA TGCTGCGGTG CTTCCACACC AAGGGGGTGA ATTACATCAA CTTCAGTGCA 
ACCGGGAAGC TCCTGGTGTC TGTGGGCGTG GATCCAGAGC ACACCATCAC TGTCTGGAGA 
TGGCAAGAAG GTACCAAGGT TGCCAGCCGA GGGGGCCACC TGGAACGAAT ATTTGTGGTG 
GAATTTCGCC CTGACTCAGA CACACAATTT GTATCTGTTG GAGTCAAACA TATGAAGTTC 
TGGACTCTGG CAGGAAGTGC TCTGCTGTAC AAGAAAGGGG TCATCGGGTC TATGGAAGCT 
GCCAAGATGC AAACGATGCT GTCCGTGGCT TTTGGTGCTA ACAACCTTAC TTTTACTGGT 
GCCATCAATG GAGATGTCTA TGTCTGGAAG GAGCACTTCC TCATCCGGCT GGTGGCCAAG 
GCTCACACAG GTCCTGTCTT TACAATGTAC ACCACCCTCC GAGATGGGCT TATTGTGACT 
GGCGGAAAAG AACGACCGAC TAAGGAAGGA GGAGCTGTAA AGTTGTGGGA CCAGGAGATG 
AAGCGCTGTC GGGCCTTCCA GCTGGAAACA GGGCAGCTGG TGGAATGTGT GCGCTCGGTG 
TGCCGTGGCA AGGGAAAAAT CTTAGTAGGA ACCAAAGATG GTGAAATAAT TGAAGTTGGG 
GAAAAAAGCG CTGCTTCAAA CATCCTGATT GATGGACACA TGGAGGGGGA GATCTGGGGC 
CTAGCCACAC ACCCCTCCAA GGACATGTTC ATCTCAGCCA GCAATGATGG CACAGCAAGG 
ATCTGGGACC TGGCTGACAA GAAACTGTTA AACAAGGTGA ACCTGGGCCA TGCGGCCAGG 
TGTGCAGCCT ATAGCCCTGA TGGGGAGATG GTGGCCATTG GCATGAAGAA TGGAGAGTTT 
GTCATCTTGC TGGTGAACAC CCTGAAAGTT TGGGGGAAAA AACGAGACCG GAAATCTGCC 
ATCCAAGATA TCAGAATCAG CCCAGACAAC AGATTCTTAG CTGTGGGGTC TTCTGAGCAG 
ACAGTTGACT TCTATGACCT TACTCAGGGC ACAAGTCTGA ACCGGATCGG CTACTGCAAA 
GACATCCCAA GCTTTGTCAT TCAGATGGAC TTTTCTGCAG ACAGCAAATA CATTCAGGTG 
TCAACAGGAG CCTATAAGCG CCAAGTACAT GAAGTTCCCC TGGGGAAGCA AGTAACTGAA 
GCCATGGTTG TTGAGAAGAT CACCTGGGCT TCCTGGACAA GTGTTCTCGG AGATGAAGTC 
ATTGGAATCT GGCCACGAAA CGCAGACAAG GCTGATGTCA ACTGTGCATG TGTGACCCAT 
GCTGGCTTGA ACATTGTCAC AGGGGATGAC TTCGGGCTGC TGAAGCTCTT TGATTTTCCA 
TGCACAGAAA AATTCGCCAA GCACAAACGT TACTTTGGTC ACTCTGCTCA TGTGACGAAC 
ATCCGCTTCT CTTCCGATGA CAAGTATGTG GTCAGCACAG GAGGAGACGA CTGCAGTGTA 
TTTGTGTGGC GATGTCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_017314517.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170006.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X6, mRNA.

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CloneID	OMu77891
Clone ID Related Accession (Same CDS sequence)	XM_017314517.1 , XM_017314517.2
Accession Version	XM_017314517.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTC CGGCTGGAGT GGGTGTACGG GTACCGGGGT CACCAGTGCC GCAACAACCT 
GTACTACACC GCCGGCAAGG AGGTGGTCTA CTTTGTAGCT GGGGTAGGGG TGGTGTACAA 
CACCCGAGAG CACAGCCAAA AATTCTTCCT GGGACACAAC GACGACATTA TCAGCATTTA 
GCCTCTGTGG GATTAGATGC CAAAAACACA GTCTGCATTT GGGACTGGAG AAAGGGAAAA 
CTTCTGGCCT CAGCTACTGG CCATTCTGAC CGGATTTTTG ATATTTCCTG GGATCCATAT 
CAGCCAAACA GAATGGTTAG CTGCGGAGTG AAGCATATAA AGTTTTGGAC ACTATGTGGA 
AATGCCCTGA CTGCAAAAAG AGGCATATTT GGCAAAACAG GAGATCTTCA GACTATCCTT 
TGCCTTGCAT GTGCCAAAGA AGACATCACT TACTCTGGTG CTTTAAACGG TGACATCTAT 
GTCTGGAAAG GGCTCACCTT AGTCCGCACT ATTCAAGGAG CACATAGTGC TGGGATCTTC 
AGCCTGTATG CTTGTGAAGA AGGCTTTGCT ACTGGTGGGC GAGATGGTTG CATACGATTA 
TGGGACACTG ATTTCAAACC AATAACCAAA ATTGATCTCA GGGAGACGGA ACAAGGATAT 
AAAGGCCTCT CTATTCGGAG TGTGTGCTGG AAAGCTGACC GCCTTCTAGC AGGAACCCAG 
GACAGTGAGA TATTTGAAGT CATTGTTCGA GAGCGAGATA AGCCAATGCT CATTCTTCAG 
GGCCACTGCG AGGGGGAACT GTGGGCTCTG GCATTGCACC CCAAGAAACC TCTGGCTGTG 
ACAGGCAGTG ATGACCGCTC TGTCAGACTG TGGAGTCTTG CTGACCATGC CTTGATTGCC 
CGCTGCAATA TGGAGGAAGC AGTTCGTAGT GTATCCTTCA GCCCAGATGG ATCTCAGTTG 
GCCCTAGGCA TGAAGGACGG CTCTTTCATT GTCCTCCGAG TCAGAGATAT GACAGAAGTC 
GTTCATATCA AGGACAGGAA AGAAGTCATT CATGAGATGA AATTCTCCCC AGATGGCTCT 
TACCTCGCAG TAGGATCCAA TGATGGCCCT GTAGATGTCT ACGCTGTTGC CCAGCGCTAT 
AAGAAAATAG GAGAATGCAA CAAGTCCCTG AGTTTCATCA CGCACATCGA CTGGTCTCTG 
GATAGTAAAT ACTTGCAAAC CAACGATGGT GCTGGGGAGA GACTGTTCTA CAAAATGCCA 
TCAGGGAAGC CTTTAACAAG TAAAGAAGAA ATCAAGGGGA TTCCCTGGGC CTCCTGGACG 
TGTGTGAGAG GCCCTGAAGT GAGTGGAATT TGGCCCAAAT ACACTGAGGT TATTGACATC 
AATTCAGTGG ACGCCAATTA TAACAGTTCA GTGTTGGTTT CTGGAGATGA TTTTGGACTG 
GTCAAATTGT TCAAATTTCC TTGTCTCAAA AAAGGAGCCA AATTTAGAAA GTATGTGGGC 
CACTCTGCAC ATGTCACCAA CGTCCGTTGG TCCCACGATT TCCAGTGGGT GTTAAGCACA 
GGAGGAGCTG ATCATTCTGT TTTCCAGTGG AGATTTATCC CAGAAGCAGT CAGCAATGGC 
GTGTTGGAAA CCACACCCCA GGAAGGTGGT GCCGACTCCT ATAGTGAGGA ATCTGACTCT 
GACTTCTCTG ACGTTCCGGA ACTGGACTCT GATATTGAGC AGGAAACTCA GATCAATTAC 
GATCGCCAGG TTTACAAAGA AGATCTACCT CAGCTAAAGC AACAAAGTAA AGAGAAAAAC 
CATGCAGTGC CATTCCTGAA GAGGGAGAAG GCTCCTGAGG ATAGCCTGAA ACTGCAGTTC 
ATACATGGTT ACAGAGGCTA TGACTGTAGA AACAATCTGT TCTACACACA AGCTGGAGAA 
GTGGTGTACC ACATTGCTGC AGTTGCTGTG GTATACAACC GGCAACAGCA CGCCCAGAGG 
TTATACCTGG GGCACGATGA TGACATTCTC AGCCTGACCA TCCATCCGGT GAAGGACTAT 
GTAGCCACTG GACAGGTTGG AAGAGATGCT GCTGTTCATG TGTGGGACAC TCAGACTCTA 
AAATGTTTGT CATTACTGAA AGGGCACCAT CAGAGAGGAG TGTGTGCTCT GGACTTTTCA 
GGGATTCGTG TGGAGAAAAA GACAAGTAGG TTGACATCAA ATGTGGGACT ACAAGAGATT 
TGGAAGAACA GATCTGACGG AAAATGTCTC GTGTCGGTTG GTTTAGACGA TTTTCACAGT 
GTTGTGTTTT GGGACTGGAA AAAGGGAGAA AAGATAGCCA CAACCAGGGG ACACAAAGAC 
AAAATATTTG TGGTAAAGTG TAATCCACAG CATGCTGACA AGTTGGTGAC AGTTGGGATT 
AAGCATATCA AGTTCTGGCA ACAAGCAGGT GGGGGCTTCA CCTCTAAAAG AGGAAGTTTT 
GGAAGTGCTG GAAAGTTGGA AACAATGATG TGTGTTTCTT ATGGGCGAAT GGAAGATCTG 
GTGTTCTCAG GTGCAGCCAC TGGAGATATT TTTATCTGGA AGGACGTTCT GCTACTGAAG 
ACAGTGAAGG CTCATGATGG GCCTGTGTTT GCCATGTATG CATTAGACAA GGGCTTTGTA 
ACAGGTGGAA AGGATGGAAT TGTGGAACTC TGGGATGATA TGTTTGAAAG ATGCTTGAAG 
ACTTATGCCA TCAAAAGGAC TGCATTGTCA ACCAGCTCAA AAGGACTGCT TTTGGAGGAT 
AACCCTTCAA TTCGTGCAAT TACCCTAGGG CATGGACACA TTCTCGTGGG GACAAAAAAT 
GGAGAGATTC TAGAAATTGA TAAGAGTGGC CCGATGACCC TGCTTGTTCA GGGACACATG 
GAAGGGGAAG TGTGGGGGCT GGCAGCACAC CCGCTCCTGC CCATCTGCGC GACAGTAAGT 
GATGACAAAA CACTTCGGAT CTGGGAATTA TCCTCCCAGC ACCGCATGCT GGCTGTACGG 
AAACTTAAAA AAGGAGGAAG ATGTTGTGCT TTCTCCCCTG ATGGGAAAGC CCTAGCAGTT 
GGATTGAATG ATGGAAGTTT CTTGGTGGTG AATGCTGACA CAGTGGAAGA CATGCTCTCC 
TTCCATCACA GAAAAGAAAT GATCTCTGAT ATAAAATTTT CAAAAGATAC AGGAAAATAC 
CTTGCTGTGG CATCTCATGA TAACTTCGTG GATATCTACA ACGTGCTTAC AAGCAAGAGG 
GTTGGCATCT GTAAAGGAGC TTCTAGTTAC ATCACACACA TTGACTGGGA CTCTAGAGGG 
AAATTACTGC AAGTTAATTC AGGTGCCAAA GAACAGCTTT TCTTTGAAGC TCCAAGAGGC 
AGAAAACACA CAATAAGGCC ATCAGAGGCT GAGAAGATAG AATGGGACAC CTGGACCTGT 
GTCCTGGGGC CCACCTGTGA AGGAATCTGG CCTGCTCACA GCGATGTGAC TGACGTCAAT 
GCTGCCAATC TTACCAAAGA TGGCTCCCTC TTAGCAACTG GGGATGACTT TGGTTTTGTG 
AAGCTTTTTT CATATCCTGT CAAGGGACAG CATGCAAGAT TTAAGAAATA TGTGGGGCAC 
AGCGCACACG TTACCAATGT GAGGTGGCTA CACAATGACT CTGTGCTGCT CACTGTAGGA 
GGTGCTGACA CAGCCTTGAT GATCTGGACC AGGGAATTTG TGGGGACGCA GGAGAGCAAG 
CTGGTTGATA GTGAGGAGTC AGACACAGAT GCAGAAGAAG ATGGAGGCTA TGACAGTGAT 
GTCGCTAGAG AGAAAGCCAT TGACTATACC ACCAAGATCT ATGCTGTGAG CATCAGGGAA 
ATGGAAGGCA CCAAACCACA CCAGCAGCTA AAAGAGGTGT CCATGGAAGA AAGGCAGGGA 
ATTGTCAAGG GATCCAGACC ACCCGTTAGC CGTGCAGCCC CCCAGCCCGA GAAACTCCAG 
AAGAACAACA TCACCAAGAA AAAGAAGCTG GTTGAGGAGC TGGCTCTGGA CCACGTGTTT 
GGCTACCGAG GATTTGACTG CCGCAACAAC CTGCATTACC TTAACGATGG TGCCGACATC 
ATTTTCCACA CTGCGGCAGC TGGCATTGTG CAGAACCTCT CCACAGGCAG CCAGAGCTTC 
TACCTGGAGC ACACGGATGA CATCCTCTGC CTCACAGTGA ATCAGCACCC AAAGTACAGA 
AATGTGGTGG CTACCAGCCA GATAGGGACA ACACCTTCAA TTCATATCTG GGATGCAATG 
ACCAAACACA CCCTTTCCAT GCTGCGGTGC TTCCACACCA AGGGGGTGAA TTACATCAAC 
TTCAGTGCAA CCGGGAAGCT CCTGGTGTCT GTGGGCGTGG ATCCAGAGCA CACCATCACT 
GTCTGGAGAT GGCAAGAAGG TACCAAGGTT GCCAGCCGAG GGGGCCACCT GGAACGAATA 
TTTGTGGTGG AATTTCGCCC TGACTCAGAC ACACAATTTG TATCTGTTGG AGTCAAACAT 
ATGAAGTTCT GGACTCTGGC AGGAAGTGCT CTGCTGTACA AGAAAGGGGT CATCGGGTCT 
ATGGAAGCTG CCAAGATGCA AACGATGCTG TCCGTGGCTT TTGGTGCTAA CAACCTTACT 
TTTACTGGTG CCATCAATGG AGATGTCTAT GTCTGGAAGG AGCACTTCCT CATCCGGCTG 
GTGGCCAAGG CTCACACAGG TCCTGTCTTT ACAATGTACA CCACCCTCCG AGATGGGCTT 
ATTGTGACTG GCGGAAAAGA ACGACCGACT AAGGAAGGAG GAGCTGTAAA GTTGTGGGAC 
CAGGAGATGA AGCGCTGTCG GGCCTTCCAG CTGGAAACAG GGCAGCTGGT GGAATGTGTG 
CGCTCGGTGT GCCGTGGCAA GGGAAAAATC TTAGTAGGAA CCAAAGATGG TGAAATAATT 
GAAGTTGGGG AAAAAAGCGC TGCTTCAAAC ATCCTGATTG ATGGACACAT GGAGGGGGAG 
ATCTGGGGCC TAGCCACACA CCCCTCCAAG GACATGTTCA TCTCAGCCAG CAATGATGGC 
ACAGCAAGGA TCTGGGACCT GGCTGACAAG AAACTGTTAA ACAAGGTGAA CCTGGGCCAT 
GCGGCCAGGT GTGCAGCCTA TAGCCCTGAT GGGGAGATGG TGGCCATTGG CATGAAGAAT 
GGAGAGTTTG TCATCTTGCT GGTGAACACC CTGAAAGTTT GGGGGAAAAA ACGAGACCGG 
AAATCTGCCA TCCAAGATAT CAGAATCAGC CCAGACAACA GATTCTTAGC TGTGGGGTCT 
TCTGAGCAGA CAGTTGACTT CTATGACCTT ACTCAGGGCA CAAGTCTGAA CCGGATCGGC 
TACTGCAAAG ACATCCCAAG CTTTGTCATT CAGATGGACT TTTCTGCAGA CAGCAAATAC 
ATTCAGGTGT CAACAGGAGC CTATAAGCGC CAAGTACATG AAGTTCCCCT GGGGAAGCAA 
GTAACTGAAG CCATGGTTGT TGAGAAGATC ACCTGGGCTT CCTGGACAAG TGTTCTCGGA 
GATGAAGTCA TTGGAATCTG GCCACGAAAC GCAGACAAGG CTGATGTCAA CTGTGCATGT 
GTGACCCATG CTGGCTTGAA CATTGTCACA GGGGATGACT TCGGGCTGCT GAAGCTCTTT 
GATTTTCCAT GCACAGAAAA ATTCGCCAAG CACAAACGTT ACTTTGGTCA CTCTGCTCAT 
GTGACGAACA TCCGCTTCTC TTCCGATGAC AAGTATGTGG TCAGCACAGG AGGAGACGAC 
TGCAGTGTAT TTGTGTGGCG ATGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170006.1
CDS	693..6482
Translation	MPAPAGVGVRVPGSPVPQQPVLHRRQGGGLLCSWGRGGVQHPRAQPKILPGTQRRHYQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPSGKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSGIRVEKKTSRLTSNVGLQEIWKNRSDGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERQGIVKGSRPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_017314517.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314517.1

ORF Insert Sequence:

ATGCCAGCTC CGGCTGGAGT GGGTGTACGG GTACCGGGGT CACCAGTGCC GCAACAACCT 
GTACTACACC GCCGGCAAGG AGGTGGTCTA CTTTGTAGCT GGGGTAGGGG TGGTGTACAA 
CACCCGAGAG CACAGCCAAA AATTCTTCCT GGGACACAAC GACGACATTA TCAGCATTTA 
GCCTCTGTGG GATTAGATGC CAAAAACACA GTCTGCATTT GGGACTGGAG AAAGGGAAAA 
CTTCTGGCCT CAGCTACTGG CCATTCTGAC CGGATTTTTG ATATTTCCTG GGATCCATAT 
CAGCCAAACA GAATGGTTAG CTGCGGAGTG AAGCATATAA AGTTTTGGAC ACTATGTGGA 
AATGCCCTGA CTGCAAAAAG AGGCATATTT GGCAAAACAG GAGATCTTCA GACTATCCTT 
TGCCTTGCAT GTGCCAAAGA AGACATCACT TACTCTGGTG CTTTAAACGG TGACATCTAT 
GTCTGGAAAG GGCTCACCTT AGTCCGCACT ATTCAAGGAG CACATAGTGC TGGGATCTTC 
AGCCTGTATG CTTGTGAAGA AGGCTTTGCT ACTGGTGGGC GAGATGGTTG CATACGATTA 
TGGGACACTG ATTTCAAACC AATAACCAAA ATTGATCTCA GGGAGACGGA ACAAGGATAT 
AAAGGCCTCT CTATTCGGAG TGTGTGCTGG AAAGCTGACC GCCTTCTAGC AGGAACCCAG 
GACAGTGAGA TATTTGAAGT CATTGTTCGA GAGCGAGATA AGCCAATGCT CATTCTTCAG 
GGCCACTGCG AGGGGGAACT GTGGGCTCTG GCATTGCACC CCAAGAAACC TCTGGCTGTG 
ACAGGCAGTG ATGACCGCTC TGTCAGACTG TGGAGTCTTG CTGACCATGC CTTGATTGCC 
CGCTGCAATA TGGAGGAAGC AGTTCGTAGT GTATCCTTCA GCCCAGATGG ATCTCAGTTG 
GCCCTAGGCA TGAAGGACGG CTCTTTCATT GTCCTCCGAG TCAGAGATAT GACAGAAGTC 
GTTCATATCA AGGACAGGAA AGAAGTCATT CATGAGATGA AATTCTCCCC AGATGGCTCT 
TACCTCGCAG TAGGATCCAA TGATGGCCCT GTAGATGTCT ACGCTGTTGC CCAGCGCTAT 
AAGAAAATAG GAGAATGCAA CAAGTCCCTG AGTTTCATCA CGCACATCGA CTGGTCTCTG 
GATAGTAAAT ACTTGCAAAC CAACGATGGT GCTGGGGAGA GACTGTTCTA CAAAATGCCA 
TCAGGGAAGC CTTTAACAAG TAAAGAAGAA ATCAAGGGGA TTCCCTGGGC CTCCTGGACG 
TGTGTGAGAG GCCCTGAAGT GAGTGGAATT TGGCCCAAAT ACACTGAGGT TATTGACATC 
AATTCAGTGG ACGCCAATTA TAACAGTTCA GTGTTGGTTT CTGGAGATGA TTTTGGACTG 
GTCAAATTGT TCAAATTTCC TTGTCTCAAA AAAGGAGCCA AATTTAGAAA GTATGTGGGC 
CACTCTGCAC ATGTCACCAA CGTCCGTTGG TCCCACGATT TCCAGTGGGT GTTAAGCACA 
GGAGGAGCTG ATCATTCTGT TTTCCAGTGG AGATTTATCC CAGAAGCAGT CAGCAATGGC 
GTGTTGGAAA CCACACCCCA GGAAGGTGGT GCCGACTCCT ATAGTGAGGA ATCTGACTCT 
GACTTCTCTG ACGTTCCGGA ACTGGACTCT GATATTGAGC AGGAAACTCA GATCAATTAC 
GATCGCCAGG TTTACAAAGA AGATCTACCT CAGCTAAAGC AACAAAGTAA AGAGAAAAAC 
CATGCAGTGC CATTCCTGAA GAGGGAGAAG GCTCCTGAGG ATAGCCTGAA ACTGCAGTTC 
ATACATGGTT ACAGAGGCTA TGACTGTAGA AACAATCTGT TCTACACACA AGCTGGAGAA 
GTGGTGTACC ACATTGCTGC AGTTGCTGTG GTATACAACC GGCAACAGCA CGCCCAGAGG 
TTATACCTGG GGCACGATGA TGACATTCTC AGCCTGACCA TCCATCCGGT GAAGGACTAT 
GTAGCCACTG GACAGGTTGG AAGAGATGCT GCTGTTCATG TGTGGGACAC TCAGACTCTA 
AAATGTTTGT CATTACTGAA AGGGCACCAT CAGAGAGGAG TGTGTGCTCT GGACTTTTCA 
GGGATTCGTG TGGAGAAAAA GACAAGTAGG TTGACATCAA ATGTGGGACT ACAAGAGATT 
TGGAAGAACA GATCTGACGG AAAATGTCTC GTGTCGGTTG GTTTAGACGA TTTTCACAGT 
GTTGTGTTTT GGGACTGGAA AAAGGGAGAA AAGATAGCCA CAACCAGGGG ACACAAAGAC 
AAAATATTTG TGGTAAAGTG TAATCCACAG CATGCTGACA AGTTGGTGAC AGTTGGGATT 
AAGCATATCA AGTTCTGGCA ACAAGCAGGT GGGGGCTTCA CCTCTAAAAG AGGAAGTTTT 
GGAAGTGCTG GAAAGTTGGA AACAATGATG TGTGTTTCTT ATGGGCGAAT GGAAGATCTG 
GTGTTCTCAG GTGCAGCCAC TGGAGATATT TTTATCTGGA AGGACGTTCT GCTACTGAAG 
ACAGTGAAGG CTCATGATGG GCCTGTGTTT GCCATGTATG CATTAGACAA GGGCTTTGTA 
ACAGGTGGAA AGGATGGAAT TGTGGAACTC TGGGATGATA TGTTTGAAAG ATGCTTGAAG 
ACTTATGCCA TCAAAAGGAC TGCATTGTCA ACCAGCTCAA AAGGACTGCT TTTGGAGGAT 
AACCCTTCAA TTCGTGCAAT TACCCTAGGG CATGGACACA TTCTCGTGGG GACAAAAAAT 
GGAGAGATTC TAGAAATTGA TAAGAGTGGC CCGATGACCC TGCTTGTTCA GGGACACATG 
GAAGGGGAAG TGTGGGGGCT GGCAGCACAC CCGCTCCTGC CCATCTGCGC GACAGTAAGT 
GATGACAAAA CACTTCGGAT CTGGGAATTA TCCTCCCAGC ACCGCATGCT GGCTGTACGG 
AAACTTAAAA AAGGAGGAAG ATGTTGTGCT TTCTCCCCTG ATGGGAAAGC CCTAGCAGTT 
GGATTGAATG ATGGAAGTTT CTTGGTGGTG AATGCTGACA CAGTGGAAGA CATGCTCTCC 
TTCCATCACA GAAAAGAAAT GATCTCTGAT ATAAAATTTT CAAAAGATAC AGGAAAATAC 
CTTGCTGTGG CATCTCATGA TAACTTCGTG GATATCTACA ACGTGCTTAC AAGCAAGAGG 
GTTGGCATCT GTAAAGGAGC TTCTAGTTAC ATCACACACA TTGACTGGGA CTCTAGAGGG 
AAATTACTGC AAGTTAATTC AGGTGCCAAA GAACAGCTTT TCTTTGAAGC TCCAAGAGGC 
AGAAAACACA CAATAAGGCC ATCAGAGGCT GAGAAGATAG AATGGGACAC CTGGACCTGT 
GTCCTGGGGC CCACCTGTGA AGGAATCTGG CCTGCTCACA GCGATGTGAC TGACGTCAAT 
GCTGCCAATC TTACCAAAGA TGGCTCCCTC TTAGCAACTG GGGATGACTT TGGTTTTGTG 
AAGCTTTTTT CATATCCTGT CAAGGGACAG CATGCAAGAT TTAAGAAATA TGTGGGGCAC 
AGCGCACACG TTACCAATGT GAGGTGGCTA CACAATGACT CTGTGCTGCT CACTGTAGGA 
GGTGCTGACA CAGCCTTGAT GATCTGGACC AGGGAATTTG TGGGGACGCA GGAGAGCAAG 
CTGGTTGATA GTGAGGAGTC AGACACAGAT GCAGAAGAAG ATGGAGGCTA TGACAGTGAT 
GTCGCTAGAG AGAAAGCCAT TGACTATACC ACCAAGATCT ATGCTGTGAG CATCAGGGAA 
ATGGAAGGCA CCAAACCACA CCAGCAGCTA AAAGAGGTGT CCATGGAAGA AAGGCAGGGA 
ATTGTCAAGG GATCCAGACC ACCCGTTAGC CGTGCAGCCC CCCAGCCCGA GAAACTCCAG 
AAGAACAACA TCACCAAGAA AAAGAAGCTG GTTGAGGAGC TGGCTCTGGA CCACGTGTTT 
GGCTACCGAG GATTTGACTG CCGCAACAAC CTGCATTACC TTAACGATGG TGCCGACATC 
ATTTTCCACA CTGCGGCAGC TGGCATTGTG CAGAACCTCT CCACAGGCAG CCAGAGCTTC 
TACCTGGAGC ACACGGATGA CATCCTCTGC CTCACAGTGA ATCAGCACCC AAAGTACAGA 
AATGTGGTGG CTACCAGCCA GATAGGGACA ACACCTTCAA TTCATATCTG GGATGCAATG 
ACCAAACACA CCCTTTCCAT GCTGCGGTGC TTCCACACCA AGGGGGTGAA TTACATCAAC 
TTCAGTGCAA CCGGGAAGCT CCTGGTGTCT GTGGGCGTGG ATCCAGAGCA CACCATCACT 
GTCTGGAGAT GGCAAGAAGG TACCAAGGTT GCCAGCCGAG GGGGCCACCT GGAACGAATA 
TTTGTGGTGG AATTTCGCCC TGACTCAGAC ACACAATTTG TATCTGTTGG AGTCAAACAT 
ATGAAGTTCT GGACTCTGGC AGGAAGTGCT CTGCTGTACA AGAAAGGGGT CATCGGGTCT 
ATGGAAGCTG CCAAGATGCA AACGATGCTG TCCGTGGCTT TTGGTGCTAA CAACCTTACT 
TTTACTGGTG CCATCAATGG AGATGTCTAT GTCTGGAAGG AGCACTTCCT CATCCGGCTG 
GTGGCCAAGG CTCACACAGG TCCTGTCTTT ACAATGTACA CCACCCTCCG AGATGGGCTT 
ATTGTGACTG GCGGAAAAGA ACGACCGACT AAGGAAGGAG GAGCTGTAAA GTTGTGGGAC 
CAGGAGATGA AGCGCTGTCG GGCCTTCCAG CTGGAAACAG GGCAGCTGGT GGAATGTGTG 
CGCTCGGTGT GCCGTGGCAA GGGAAAAATC TTAGTAGGAA CCAAAGATGG TGAAATAATT 
GAAGTTGGGG AAAAAAGCGC TGCTTCAAAC ATCCTGATTG ATGGACACAT GGAGGGGGAG 
ATCTGGGGCC TAGCCACACA CCCCTCCAAG GACATGTTCA TCTCAGCCAG CAATGATGGC 
ACAGCAAGGA TCTGGGACCT GGCTGACAAG AAACTGTTAA ACAAGGTGAA CCTGGGCCAT 
GCGGCCAGGT GTGCAGCCTA TAGCCCTGAT GGGGAGATGG TGGCCATTGG CATGAAGAAT 
GGAGAGTTTG TCATCTTGCT GGTGAACACC CTGAAAGTTT GGGGGAAAAA ACGAGACCGG 
AAATCTGCCA TCCAAGATAT CAGAATCAGC CCAGACAACA GATTCTTAGC TGTGGGGTCT 
TCTGAGCAGA CAGTTGACTT CTATGACCTT ACTCAGGGCA CAAGTCTGAA CCGGATCGGC 
TACTGCAAAG ACATCCCAAG CTTTGTCATT CAGATGGACT TTTCTGCAGA CAGCAAATAC 
ATTCAGGTGT CAACAGGAGC CTATAAGCGC CAAGTACATG AAGTTCCCCT GGGGAAGCAA 
GTAACTGAAG CCATGGTTGT TGAGAAGATC ACCTGGGCTT CCTGGACAAG TGTTCTCGGA 
GATGAAGTCA TTGGAATCTG GCCACGAAAC GCAGACAAGG CTGATGTCAA CTGTGCATGT 
GTGACCCATG CTGGCTTGAA CATTGTCACA GGGGATGACT TCGGGCTGCT GAAGCTCTTT 
GATTTTCCAT GCACAGAAAA ATTCGCCAAG CACAAACGTT ACTTTGGTCA CTCTGCTCAT 
GTGACGAACA TCCGCTTCTC TTCCGATGAC AAGTATGTGG TCAGCACAGG AGGAGACGAC 
TGCAGTGTAT TTGTGTGGCG ATGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_017314517.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170006.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X6, mRNA.

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CloneID	OMu77891
Clone ID Related Accession (Same CDS sequence)	XM_017314517.1 , XM_017314517.2
Accession Version	XM_017314517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTC CGGCTGGAGT GGGTGTACGG GTACCGGGGT CACCAGTGCC GCAACAACCT 
GTACTACACC GCCGGCAAGG AGGTGGTCTA CTTTGTAGCT GGGGTAGGGG TGGTGTACAA 
CACCCGAGAG CACAGCCAAA AATTCTTCCT GGGACACAAC GACGACATTA TCAGCATTTA 
GCCTCTGTGG GATTAGATGC CAAAAACACA GTCTGCATTT GGGACTGGAG AAAGGGAAAA 
CTTCTGGCCT CAGCTACTGG CCATTCTGAC CGGATTTTTG ATATTTCCTG GGATCCATAT 
CAGCCAAACA GAATGGTTAG CTGCGGAGTG AAGCATATAA AGTTTTGGAC ACTATGTGGA 
AATGCCCTGA CTGCAAAAAG AGGCATATTT GGCAAAACAG GAGATCTTCA GACTATCCTT 
TGCCTTGCAT GTGCCAAAGA AGACATCACT TACTCTGGTG CTTTAAACGG TGACATCTAT 
GTCTGGAAAG GGCTCACCTT AGTCCGCACT ATTCAAGGAG CACATAGTGC TGGGATCTTC 
AGCCTGTATG CTTGTGAAGA AGGCTTTGCT ACTGGTGGGC GAGATGGTTG CATACGATTA 
TGGGACACTG ATTTCAAACC AATAACCAAA ATTGATCTCA GGGAGACGGA ACAAGGATAT 
AAAGGCCTCT CTATTCGGAG TGTGTGCTGG AAAGCTGACC GCCTTCTAGC AGGAACCCAG 
GACAGTGAGA TATTTGAAGT CATTGTTCGA GAGCGAGATA AGCCAATGCT CATTCTTCAG 
GGCCACTGCG AGGGGGAACT GTGGGCTCTG GCATTGCACC CCAAGAAACC TCTGGCTGTG 
ACAGGCAGTG ATGACCGCTC TGTCAGACTG TGGAGTCTTG CTGACCATGC CTTGATTGCC 
CGCTGCAATA TGGAGGAAGC AGTTCGTAGT GTATCCTTCA GCCCAGATGG ATCTCAGTTG 
GCCCTAGGCA TGAAGGACGG CTCTTTCATT GTCCTCCGAG TCAGAGATAT GACAGAAGTC 
GTTCATATCA AGGACAGGAA AGAAGTCATT CATGAGATGA AATTCTCCCC AGATGGCTCT 
TACCTCGCAG TAGGATCCAA TGATGGCCCT GTAGATGTCT ACGCTGTTGC CCAGCGCTAT 
AAGAAAATAG GAGAATGCAA CAAGTCCCTG AGTTTCATCA CGCACATCGA CTGGTCTCTG 
GATAGTAAAT ACTTGCAAAC CAACGATGGT GCTGGGGAGA GACTGTTCTA CAAAATGCCA 
TCAGGGAAGC CTTTAACAAG TAAAGAAGAA ATCAAGGGGA TTCCCTGGGC CTCCTGGACG 
TGTGTGAGAG GCCCTGAAGT GAGTGGAATT TGGCCCAAAT ACACTGAGGT TATTGACATC 
AATTCAGTGG ACGCCAATTA TAACAGTTCA GTGTTGGTTT CTGGAGATGA TTTTGGACTG 
GTCAAATTGT TCAAATTTCC TTGTCTCAAA AAAGGAGCCA AATTTAGAAA GTATGTGGGC 
CACTCTGCAC ATGTCACCAA CGTCCGTTGG TCCCACGATT TCCAGTGGGT GTTAAGCACA 
GGAGGAGCTG ATCATTCTGT TTTCCAGTGG AGATTTATCC CAGAAGCAGT CAGCAATGGC 
GTGTTGGAAA CCACACCCCA GGAAGGTGGT GCCGACTCCT ATAGTGAGGA ATCTGACTCT 
GACTTCTCTG ACGTTCCGGA ACTGGACTCT GATATTGAGC AGGAAACTCA GATCAATTAC 
GATCGCCAGG TTTACAAAGA AGATCTACCT CAGCTAAAGC AACAAAGTAA AGAGAAAAAC 
CATGCAGTGC CATTCCTGAA GAGGGAGAAG GCTCCTGAGG ATAGCCTGAA ACTGCAGTTC 
ATACATGGTT ACAGAGGCTA TGACTGTAGA AACAATCTGT TCTACACACA AGCTGGAGAA 
GTGGTGTACC ACATTGCTGC AGTTGCTGTG GTATACAACC GGCAACAGCA CGCCCAGAGG 
TTATACCTGG GGCACGATGA TGACATTCTC AGCCTGACCA TCCATCCGGT GAAGGACTAT 
GTAGCCACTG GACAGGTTGG AAGAGATGCT GCTGTTCATG TGTGGGACAC TCAGACTCTA 
AAATGTTTGT CATTACTGAA AGGGCACCAT CAGAGAGGAG TGTGTGCTCT GGACTTTTCA 
GGGATTCGTG TGGAGAAAAA GACAAGTAGG TTGACATCAA ATGTGGGACT ACAAGAGATT 
TGGAAGAACA GATCTGACGG AAAATGTCTC GTGTCGGTTG GTTTAGACGA TTTTCACAGT 
GTTGTGTTTT GGGACTGGAA AAAGGGAGAA AAGATAGCCA CAACCAGGGG ACACAAAGAC 
AAAATATTTG TGGTAAAGTG TAATCCACAG CATGCTGACA AGTTGGTGAC AGTTGGGATT 
AAGCATATCA AGTTCTGGCA ACAAGCAGGT GGGGGCTTCA CCTCTAAAAG AGGAAGTTTT 
GGAAGTGCTG GAAAGTTGGA AACAATGATG TGTGTTTCTT ATGGGCGAAT GGAAGATCTG 
GTGTTCTCAG GTGCAGCCAC TGGAGATATT TTTATCTGGA AGGACGTTCT GCTACTGAAG 
ACAGTGAAGG CTCATGATGG GCCTGTGTTT GCCATGTATG CATTAGACAA GGGCTTTGTA 
ACAGGTGGAA AGGATGGAAT TGTGGAACTC TGGGATGATA TGTTTGAAAG ATGCTTGAAG 
ACTTATGCCA TCAAAAGGAC TGCATTGTCA ACCAGCTCAA AAGGACTGCT TTTGGAGGAT 
AACCCTTCAA TTCGTGCAAT TACCCTAGGG CATGGACACA TTCTCGTGGG GACAAAAAAT 
GGAGAGATTC TAGAAATTGA TAAGAGTGGC CCGATGACCC TGCTTGTTCA GGGACACATG 
GAAGGGGAAG TGTGGGGGCT GGCAGCACAC CCGCTCCTGC CCATCTGCGC GACAGTAAGT 
GATGACAAAA CACTTCGGAT CTGGGAATTA TCCTCCCAGC ACCGCATGCT GGCTGTACGG 
AAACTTAAAA AAGGAGGAAG ATGTTGTGCT TTCTCCCCTG ATGGGAAAGC CCTAGCAGTT 
GGATTGAATG ATGGAAGTTT CTTGGTGGTG AATGCTGACA CAGTGGAAGA CATGCTCTCC 
TTCCATCACA GAAAAGAAAT GATCTCTGAT ATAAAATTTT CAAAAGATAC AGGAAAATAC 
CTTGCTGTGG CATCTCATGA TAACTTCGTG GATATCTACA ACGTGCTTAC AAGCAAGAGG 
GTTGGCATCT GTAAAGGAGC TTCTAGTTAC ATCACACACA TTGACTGGGA CTCTAGAGGG 
AAATTACTGC AAGTTAATTC AGGTGCCAAA GAACAGCTTT TCTTTGAAGC TCCAAGAGGC 
AGAAAACACA CAATAAGGCC ATCAGAGGCT GAGAAGATAG AATGGGACAC CTGGACCTGT 
GTCCTGGGGC CCACCTGTGA AGGAATCTGG CCTGCTCACA GCGATGTGAC TGACGTCAAT 
GCTGCCAATC TTACCAAAGA TGGCTCCCTC TTAGCAACTG GGGATGACTT TGGTTTTGTG 
AAGCTTTTTT CATATCCTGT CAAGGGACAG CATGCAAGAT TTAAGAAATA TGTGGGGCAC 
AGCGCACACG TTACCAATGT GAGGTGGCTA CACAATGACT CTGTGCTGCT CACTGTAGGA 
GGTGCTGACA CAGCCTTGAT GATCTGGACC AGGGAATTTG TGGGGACGCA GGAGAGCAAG 
CTGGTTGATA GTGAGGAGTC AGACACAGAT GCAGAAGAAG ATGGAGGCTA TGACAGTGAT 
GTCGCTAGAG AGAAAGCCAT TGACTATACC ACCAAGATCT ATGCTGTGAG CATCAGGGAA 
ATGGAAGGCA CCAAACCACA CCAGCAGCTA AAAGAGGTGT CCATGGAAGA AAGGCAGGGA 
ATTGTCAAGG GATCCAGACC ACCCGTTAGC CGTGCAGCCC CCCAGCCCGA GAAACTCCAG 
AAGAACAACA TCACCAAGAA AAAGAAGCTG GTTGAGGAGC TGGCTCTGGA CCACGTGTTT 
GGCTACCGAG GATTTGACTG CCGCAACAAC CTGCATTACC TTAACGATGG TGCCGACATC 
ATTTTCCACA CTGCGGCAGC TGGCATTGTG CAGAACCTCT CCACAGGCAG CCAGAGCTTC 
TACCTGGAGC ACACGGATGA CATCCTCTGC CTCACAGTGA ATCAGCACCC AAAGTACAGA 
AATGTGGTGG CTACCAGCCA GATAGGGACA ACACCTTCAA TTCATATCTG GGATGCAATG 
ACCAAACACA CCCTTTCCAT GCTGCGGTGC TTCCACACCA AGGGGGTGAA TTACATCAAC 
TTCAGTGCAA CCGGGAAGCT CCTGGTGTCT GTGGGCGTGG ATCCAGAGCA CACCATCACT 
GTCTGGAGAT GGCAAGAAGG TACCAAGGTT GCCAGCCGAG GGGGCCACCT GGAACGAATA 
TTTGTGGTGG AATTTCGCCC TGACTCAGAC ACACAATTTG TATCTGTTGG AGTCAAACAT 
ATGAAGTTCT GGACTCTGGC AGGAAGTGCT CTGCTGTACA AGAAAGGGGT CATCGGGTCT 
ATGGAAGCTG CCAAGATGCA AACGATGCTG TCCGTGGCTT TTGGTGCTAA CAACCTTACT 
TTTACTGGTG CCATCAATGG AGATGTCTAT GTCTGGAAGG AGCACTTCCT CATCCGGCTG 
GTGGCCAAGG CTCACACAGG TCCTGTCTTT ACAATGTACA CCACCCTCCG AGATGGGCTT 
ATTGTGACTG GCGGAAAAGA ACGACCGACT AAGGAAGGAG GAGCTGTAAA GTTGTGGGAC 
CAGGAGATGA AGCGCTGTCG GGCCTTCCAG CTGGAAACAG GGCAGCTGGT GGAATGTGTG 
CGCTCGGTGT GCCGTGGCAA GGGAAAAATC TTAGTAGGAA CCAAAGATGG TGAAATAATT 
GAAGTTGGGG AAAAAAGCGC TGCTTCAAAC ATCCTGATTG ATGGACACAT GGAGGGGGAG 
ATCTGGGGCC TAGCCACACA CCCCTCCAAG GACATGTTCA TCTCAGCCAG CAATGATGGC 
ACAGCAAGGA TCTGGGACCT GGCTGACAAG AAACTGTTAA ACAAGGTGAA CCTGGGCCAT 
GCGGCCAGGT GTGCAGCCTA TAGCCCTGAT GGGGAGATGG TGGCCATTGG CATGAAGAAT 
GGAGAGTTTG TCATCTTGCT GGTGAACACC CTGAAAGTTT GGGGGAAAAA ACGAGACCGG 
AAATCTGCCA TCCAAGATAT CAGAATCAGC CCAGACAACA GATTCTTAGC TGTGGGGTCT 
TCTGAGCAGA CAGTTGACTT CTATGACCTT ACTCAGGGCA CAAGTCTGAA CCGGATCGGC 
TACTGCAAAG ACATCCCAAG CTTTGTCATT CAGATGGACT TTTCTGCAGA CAGCAAATAC 
ATTCAGGTGT CAACAGGAGC CTATAAGCGC CAAGTACATG AAGTTCCCCT GGGGAAGCAA 
GTAACTGAAG CCATGGTTGT TGAGAAGATC ACCTGGGCTT CCTGGACAAG TGTTCTCGGA 
GATGAAGTCA TTGGAATCTG GCCACGAAAC GCAGACAAGG CTGATGTCAA CTGTGCATGT 
GTGACCCATG CTGGCTTGAA CATTGTCACA GGGGATGACT TCGGGCTGCT GAAGCTCTTT 
GATTTTCCAT GCACAGAAAA ATTCGCCAAG CACAAACGTT ACTTTGGTCA CTCTGCTCAT 
GTGACGAACA TCCGCTTCTC TTCCGATGAC AAGTATGTGG TCAGCACAGG AGGAGACGAC 
TGCAGTGTAT TTGTGTGGCG ATGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170006.1
CDS	704..6493
Translation	MPAPAGVGVRVPGSPVPQQPVLHRRQGGGLLCSWGRGGVQHPRAQPKILPGTQRRHYQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPSGKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSGIRVEKKTSRLTSNVGLQEIWKNRSDGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERQGIVKGSRPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_017314517.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314517.2

ORF Insert Sequence:

ATGCCAGCTC CGGCTGGAGT GGGTGTACGG GTACCGGGGT CACCAGTGCC GCAACAACCT 
GTACTACACC GCCGGCAAGG AGGTGGTCTA CTTTGTAGCT GGGGTAGGGG TGGTGTACAA 
CACCCGAGAG CACAGCCAAA AATTCTTCCT GGGACACAAC GACGACATTA TCAGCATTTA 
GCCTCTGTGG GATTAGATGC CAAAAACACA GTCTGCATTT GGGACTGGAG AAAGGGAAAA 
CTTCTGGCCT CAGCTACTGG CCATTCTGAC CGGATTTTTG ATATTTCCTG GGATCCATAT 
CAGCCAAACA GAATGGTTAG CTGCGGAGTG AAGCATATAA AGTTTTGGAC ACTATGTGGA 
AATGCCCTGA CTGCAAAAAG AGGCATATTT GGCAAAACAG GAGATCTTCA GACTATCCTT 
TGCCTTGCAT GTGCCAAAGA AGACATCACT TACTCTGGTG CTTTAAACGG TGACATCTAT 
GTCTGGAAAG GGCTCACCTT AGTCCGCACT ATTCAAGGAG CACATAGTGC TGGGATCTTC 
AGCCTGTATG CTTGTGAAGA AGGCTTTGCT ACTGGTGGGC GAGATGGTTG CATACGATTA 
TGGGACACTG ATTTCAAACC AATAACCAAA ATTGATCTCA GGGAGACGGA ACAAGGATAT 
AAAGGCCTCT CTATTCGGAG TGTGTGCTGG AAAGCTGACC GCCTTCTAGC AGGAACCCAG 
GACAGTGAGA TATTTGAAGT CATTGTTCGA GAGCGAGATA AGCCAATGCT CATTCTTCAG 
GGCCACTGCG AGGGGGAACT GTGGGCTCTG GCATTGCACC CCAAGAAACC TCTGGCTGTG 
ACAGGCAGTG ATGACCGCTC TGTCAGACTG TGGAGTCTTG CTGACCATGC CTTGATTGCC 
CGCTGCAATA TGGAGGAAGC AGTTCGTAGT GTATCCTTCA GCCCAGATGG ATCTCAGTTG 
GCCCTAGGCA TGAAGGACGG CTCTTTCATT GTCCTCCGAG TCAGAGATAT GACAGAAGTC 
GTTCATATCA AGGACAGGAA AGAAGTCATT CATGAGATGA AATTCTCCCC AGATGGCTCT 
TACCTCGCAG TAGGATCCAA TGATGGCCCT GTAGATGTCT ACGCTGTTGC CCAGCGCTAT 
AAGAAAATAG GAGAATGCAA CAAGTCCCTG AGTTTCATCA CGCACATCGA CTGGTCTCTG 
GATAGTAAAT ACTTGCAAAC CAACGATGGT GCTGGGGAGA GACTGTTCTA CAAAATGCCA 
TCAGGGAAGC CTTTAACAAG TAAAGAAGAA ATCAAGGGGA TTCCCTGGGC CTCCTGGACG 
TGTGTGAGAG GCCCTGAAGT GAGTGGAATT TGGCCCAAAT ACACTGAGGT TATTGACATC 
AATTCAGTGG ACGCCAATTA TAACAGTTCA GTGTTGGTTT CTGGAGATGA TTTTGGACTG 
GTCAAATTGT TCAAATTTCC TTGTCTCAAA AAAGGAGCCA AATTTAGAAA GTATGTGGGC 
CACTCTGCAC ATGTCACCAA CGTCCGTTGG TCCCACGATT TCCAGTGGGT GTTAAGCACA 
GGAGGAGCTG ATCATTCTGT TTTCCAGTGG AGATTTATCC CAGAAGCAGT CAGCAATGGC 
GTGTTGGAAA CCACACCCCA GGAAGGTGGT GCCGACTCCT ATAGTGAGGA ATCTGACTCT 
GACTTCTCTG ACGTTCCGGA ACTGGACTCT GATATTGAGC AGGAAACTCA GATCAATTAC 
GATCGCCAGG TTTACAAAGA AGATCTACCT CAGCTAAAGC AACAAAGTAA AGAGAAAAAC 
CATGCAGTGC CATTCCTGAA GAGGGAGAAG GCTCCTGAGG ATAGCCTGAA ACTGCAGTTC 
ATACATGGTT ACAGAGGCTA TGACTGTAGA AACAATCTGT TCTACACACA AGCTGGAGAA 
GTGGTGTACC ACATTGCTGC AGTTGCTGTG GTATACAACC GGCAACAGCA CGCCCAGAGG 
TTATACCTGG GGCACGATGA TGACATTCTC AGCCTGACCA TCCATCCGGT GAAGGACTAT 
GTAGCCACTG GACAGGTTGG AAGAGATGCT GCTGTTCATG TGTGGGACAC TCAGACTCTA 
AAATGTTTGT CATTACTGAA AGGGCACCAT CAGAGAGGAG TGTGTGCTCT GGACTTTTCA 
GGGATTCGTG TGGAGAAAAA GACAAGTAGG TTGACATCAA ATGTGGGACT ACAAGAGATT 
TGGAAGAACA GATCTGACGG AAAATGTCTC GTGTCGGTTG GTTTAGACGA TTTTCACAGT 
GTTGTGTTTT GGGACTGGAA AAAGGGAGAA AAGATAGCCA CAACCAGGGG ACACAAAGAC 
AAAATATTTG TGGTAAAGTG TAATCCACAG CATGCTGACA AGTTGGTGAC AGTTGGGATT 
AAGCATATCA AGTTCTGGCA ACAAGCAGGT GGGGGCTTCA CCTCTAAAAG AGGAAGTTTT 
GGAAGTGCTG GAAAGTTGGA AACAATGATG TGTGTTTCTT ATGGGCGAAT GGAAGATCTG 
GTGTTCTCAG GTGCAGCCAC TGGAGATATT TTTATCTGGA AGGACGTTCT GCTACTGAAG 
ACAGTGAAGG CTCATGATGG GCCTGTGTTT GCCATGTATG CATTAGACAA GGGCTTTGTA 
ACAGGTGGAA AGGATGGAAT TGTGGAACTC TGGGATGATA TGTTTGAAAG ATGCTTGAAG 
ACTTATGCCA TCAAAAGGAC TGCATTGTCA ACCAGCTCAA AAGGACTGCT TTTGGAGGAT 
AACCCTTCAA TTCGTGCAAT TACCCTAGGG CATGGACACA TTCTCGTGGG GACAAAAAAT 
GGAGAGATTC TAGAAATTGA TAAGAGTGGC CCGATGACCC TGCTTGTTCA GGGACACATG 
GAAGGGGAAG TGTGGGGGCT GGCAGCACAC CCGCTCCTGC CCATCTGCGC GACAGTAAGT 
GATGACAAAA CACTTCGGAT CTGGGAATTA TCCTCCCAGC ACCGCATGCT GGCTGTACGG 
AAACTTAAAA AAGGAGGAAG ATGTTGTGCT TTCTCCCCTG ATGGGAAAGC CCTAGCAGTT 
GGATTGAATG ATGGAAGTTT CTTGGTGGTG AATGCTGACA CAGTGGAAGA CATGCTCTCC 
TTCCATCACA GAAAAGAAAT GATCTCTGAT ATAAAATTTT CAAAAGATAC AGGAAAATAC 
CTTGCTGTGG CATCTCATGA TAACTTCGTG GATATCTACA ACGTGCTTAC AAGCAAGAGG 
GTTGGCATCT GTAAAGGAGC TTCTAGTTAC ATCACACACA TTGACTGGGA CTCTAGAGGG 
AAATTACTGC AAGTTAATTC AGGTGCCAAA GAACAGCTTT TCTTTGAAGC TCCAAGAGGC 
AGAAAACACA CAATAAGGCC ATCAGAGGCT GAGAAGATAG AATGGGACAC CTGGACCTGT 
GTCCTGGGGC CCACCTGTGA AGGAATCTGG CCTGCTCACA GCGATGTGAC TGACGTCAAT 
GCTGCCAATC TTACCAAAGA TGGCTCCCTC TTAGCAACTG GGGATGACTT TGGTTTTGTG 
AAGCTTTTTT CATATCCTGT CAAGGGACAG CATGCAAGAT TTAAGAAATA TGTGGGGCAC 
AGCGCACACG TTACCAATGT GAGGTGGCTA CACAATGACT CTGTGCTGCT CACTGTAGGA 
GGTGCTGACA CAGCCTTGAT GATCTGGACC AGGGAATTTG TGGGGACGCA GGAGAGCAAG 
CTGGTTGATA GTGAGGAGTC AGACACAGAT GCAGAAGAAG ATGGAGGCTA TGACAGTGAT 
GTCGCTAGAG AGAAAGCCAT TGACTATACC ACCAAGATCT ATGCTGTGAG CATCAGGGAA 
ATGGAAGGCA CCAAACCACA CCAGCAGCTA AAAGAGGTGT CCATGGAAGA AAGGCAGGGA 
ATTGTCAAGG GATCCAGACC ACCCGTTAGC CGTGCAGCCC CCCAGCCCGA GAAACTCCAG 
AAGAACAACA TCACCAAGAA AAAGAAGCTG GTTGAGGAGC TGGCTCTGGA CCACGTGTTT 
GGCTACCGAG GATTTGACTG CCGCAACAAC CTGCATTACC TTAACGATGG TGCCGACATC 
ATTTTCCACA CTGCGGCAGC TGGCATTGTG CAGAACCTCT CCACAGGCAG CCAGAGCTTC 
TACCTGGAGC ACACGGATGA CATCCTCTGC CTCACAGTGA ATCAGCACCC AAAGTACAGA 
AATGTGGTGG CTACCAGCCA GATAGGGACA ACACCTTCAA TTCATATCTG GGATGCAATG 
ACCAAACACA CCCTTTCCAT GCTGCGGTGC TTCCACACCA AGGGGGTGAA TTACATCAAC 
TTCAGTGCAA CCGGGAAGCT CCTGGTGTCT GTGGGCGTGG ATCCAGAGCA CACCATCACT 
GTCTGGAGAT GGCAAGAAGG TACCAAGGTT GCCAGCCGAG GGGGCCACCT GGAACGAATA 
TTTGTGGTGG AATTTCGCCC TGACTCAGAC ACACAATTTG TATCTGTTGG AGTCAAACAT 
ATGAAGTTCT GGACTCTGGC AGGAAGTGCT CTGCTGTACA AGAAAGGGGT CATCGGGTCT 
ATGGAAGCTG CCAAGATGCA AACGATGCTG TCCGTGGCTT TTGGTGCTAA CAACCTTACT 
TTTACTGGTG CCATCAATGG AGATGTCTAT GTCTGGAAGG AGCACTTCCT CATCCGGCTG 
GTGGCCAAGG CTCACACAGG TCCTGTCTTT ACAATGTACA CCACCCTCCG AGATGGGCTT 
ATTGTGACTG GCGGAAAAGA ACGACCGACT AAGGAAGGAG GAGCTGTAAA GTTGTGGGAC 
CAGGAGATGA AGCGCTGTCG GGCCTTCCAG CTGGAAACAG GGCAGCTGGT GGAATGTGTG 
CGCTCGGTGT GCCGTGGCAA GGGAAAAATC TTAGTAGGAA CCAAAGATGG TGAAATAATT 
GAAGTTGGGG AAAAAAGCGC TGCTTCAAAC ATCCTGATTG ATGGACACAT GGAGGGGGAG 
ATCTGGGGCC TAGCCACACA CCCCTCCAAG GACATGTTCA TCTCAGCCAG CAATGATGGC 
ACAGCAAGGA TCTGGGACCT GGCTGACAAG AAACTGTTAA ACAAGGTGAA CCTGGGCCAT 
GCGGCCAGGT GTGCAGCCTA TAGCCCTGAT GGGGAGATGG TGGCCATTGG CATGAAGAAT 
GGAGAGTTTG TCATCTTGCT GGTGAACACC CTGAAAGTTT GGGGGAAAAA ACGAGACCGG 
AAATCTGCCA TCCAAGATAT CAGAATCAGC CCAGACAACA GATTCTTAGC TGTGGGGTCT 
TCTGAGCAGA CAGTTGACTT CTATGACCTT ACTCAGGGCA CAAGTCTGAA CCGGATCGGC 
TACTGCAAAG ACATCCCAAG CTTTGTCATT CAGATGGACT TTTCTGCAGA CAGCAAATAC 
ATTCAGGTGT CAACAGGAGC CTATAAGCGC CAAGTACATG AAGTTCCCCT GGGGAAGCAA 
GTAACTGAAG CCATGGTTGT TGAGAAGATC ACCTGGGCTT CCTGGACAAG TGTTCTCGGA 
GATGAAGTCA TTGGAATCTG GCCACGAAAC GCAGACAAGG CTGATGTCAA CTGTGCATGT 
GTGACCCATG CTGGCTTGAA CATTGTCACA GGGGATGACT TCGGGCTGCT GAAGCTCTTT 
GATTTTCCAT GCACAGAAAA ATTCGCCAAG CACAAACGTT ACTTTGGTCA CTCTGCTCAT 
GTGACGAACA TCCGCTTCTC TTCCGATGAC AAGTATGTGG TCAGCACAGG AGGAGACGAC 
TGCAGTGTAT TTGTGTGGCG ATGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( NM_146016.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666128.2 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), mRNA.

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CloneID	OMu20880
Clone ID Related Accession (Same CDS sequence)	NM_146016.2
Accession Version	NM_146016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY740982.1, BB633761.1, AK049034.1, BB395696.2, BC024726.1 and AK081418.1. On Mar 9, 2007 this sequence version replaced NM_146016.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGCTGACG GAAAATGTCT CGTGTCGGTT GGTTTAGACG ATTTTCACAG TGTTGTGTTT 
TGGGACTGGA AAAAGGGAGA AAAGATAGCC ACAACCAGGG GACACAAAGA CAAAATATTT 
GTGGTAAAGT GTAATCCACA GCATGCTGAC AAGTTGGTGA CAGTTGGGAT TAAGCATATC 
AAGTTCTGGC AACAAGCAGG TGGGGGCTTC ACCTCTAAAA GAGGAAGTTT TGGAAGTGCT 
GGAAAGTTGG AAACAATGAT GTGTGTTTCT TATGGGCGAA TGGAAGATCT GGTGTTCTCA 
GGTGCAGCCA CTGGAGATAT TTTTATCTGG AAGGACGTTC TGCTACTGAA GACAGTGAAG 
GCTCATGATG GGCCTGTGTT TGCCATGTAT GCATTAGACA AGGGCTTTGT AACAGGTGGA 
AAGGATGGAA TTGTGGAACT CTGGGATGAT ATGTTTGAAA GATGCTTGAA GACTTATGCC 
ATCAAAAGGA CTGCATTGTC AACCAGCTCA AAAGGACTGC TTTTGGAGGA TAACCCTTCA 
ATTCGTGCAA TTACCCTAGG GCATGGACAC ATTCTCGTGG GGACAAAAAA TGGAGAGATT 
CTAGAAATTG ATAAGAGTGG CCCGATGACC CTGCTTGTTC AGGGACACAT GGAAGGGGAA 
GTGTGGGGGC TGGCAGCACA CCCGCTCCTG CCCATCTGCG CGACAGTAAG TGATGACAAA 
ACACTTCGGA TCTGGGAATT ATCCTCCCAG CACCGCATGC TGGCTGTACG GAAACTTAAA 
AAAGGAGGAA GATGTTGTGC TTTCTCCCCT GATGGGAAAG CCCTAGCAGT TGGATTGAAT 
GATGGAAGTT TCTTGGTGGT GAATGCTGAC ACAGTGGAAG ACATGCTCTC CTTCCATCAC 
AGAAAAGAAA TGATCTCTGA TATAAAATTT TCAAAAGATA CAGGAAAATA CCTTGCTGTG 
GCATCTCATG ATAACTTCGT GGATATCTAC AACGTGCTTA CAAGCAAGAG GGTTGGCATC 
TGTAAAGGAG CTTCTAGTTA CATCACACAC ATTGACTGGG ACTCTAGAGG GAAATTACTG 
CAAGTTAATT CAGGTGCCAA AGAACAGCTT TTCTTTGAAG CTCCAAGAGG CAGAAAACAC 
ACAATAAGGC CATCAGAGGC TGAGAAGATA GAATGGGACA CCTGGACCTG TGTCCTGGGG 
CCCACCTGTG AAGGAATCTG GCCTGCTCAC AGCGATGTGA CTGACGTCAA TGCTGCCAAT 
CTTACCAAAG ATGGCTCCCT CTTAGCAACT GGGGATGACT TTGGTTTTGT GAAGCTTTTT 
TCATATCCTG TCAAGGGACA GCATGCAAGA TTTAAGAAAT ATGTGGGGCA CAGCGCACAC 
GTTACCAATG TGAGGTGGCT ACACAATGAC TCTGTGCTGC TCACTGTAGG AGGTGCTGAC 
ACAGCCTTGA TGATCTGGAC CAGGGAATTT GTGGGGACGC AGGAGAGCAA GCTGGTTGAT 
AGTGAGGAGT CAGACACAGA TGCAGAAGAA GATGGAGGCT ATGACAGTGA TGTCGCTAGA 
GAGAAAGCCA TTGACTATAC CACCAAGATC TATGCTGTGA GCATCAGGGA AATGGAAGGC 
ACCAAACCAC ACCAGCAGCT AAAAGAGGTG TCCATGGAAG AAAGACCACC CGTTAGCCGT 
GCAGCCCCCC AGCCCGAGAA ACTCCAGAAG AACAACATCA CCAAGAAAAA GAAGCTGGTT 
GAGGAGCTGG CTCTGGACCA CGTGTTTGGC TACCGAGGAT TTGACTGCCG CAACAACCTG 
CATTACCTTA ACGATGGTGC CGACATCATT TTCCACACTG CGGCAGCTGG CATTGTGCAG 
AACCTCTCCA CAGGCAGCCA GAGCTTCTAC CTGGAGCACA CGGATGACAT CCTCTGCCTC 
ACAGTGAATC AGCACCCAAA GTACAGAAAT GTGGTGGCTA CCAGCCAGAT AGGGACAACA 
CCTTCAATTC ATATCTGGGA TGCAATGACC AAACACACCC TTTCCATGCT GCGGTGCTTC 
CACACCAAGG GGGTGAATTA CATCAACTTC AGTGCAACCG GGAAGCTCCT GGTGTCTGTG 
GGCGTGGATC CAGAGCACAC CATCACTGTC TGGAGATGGC AAGAAGGTAC CAAGGTTGCC 
AGCCGAGGGG GCCACCTGGA ACGAATATTT GTGGTGGAAT TTCGCCCTGA CTCAGACACA 
CAATTTGTAT CTGTTGGAGT CAAACATATG AAGTTCTGGA CTCTGGCAGG AAGTGCTCTG 
CTGTACAAGA AAGGGGTCAT CGGGTCTATG GAAGCTGCCA AGATGCAAAC GATGCTGTCC 
GTGGCTTTTG GTGCTAACAA CCTTACTTTT ACTGGTGCCA TCAATGGAGA TGTCTATGTC 
TGGAAGGAGC ACTTCCTCAT CCGGCTGGTG GCCAAGGCTC ACACAGGTCC TGTCTTTACA 
ATGTACACCA CCCTCCGAGA TGGGCTTATT GTGACTGGCG GAAAAGAACG ACCGACTAAG 
GAAGGAGGAG CTGTAAAGTT GTGGGACCAG GAGATGAAGC GCTGTCGGGC CTTCCAGCTG 
GAAACAGGGC AGCTGGTGGA ATGTGTGCGC TCGGTGTGCC GTGGCAAGGG AAAAATCTTA 
GTAGGAACCA AAGATGGTGA AATAATTGAA GTTGGGGAAA AAAGCGCTGC TTCAAACATC 
CTGATTGATG GACACATGGA GGGGGAGATC TGGGGCCTAG CCACACACCC CTCCAAGGAC 
ATGTTCATCT CAGCCAGCAA TGATGGCACA GCAAGGATCT GGGACCTGGC TGACAAGAAA 
CTGTTAAACA AGGTGAACCT GGGCCATGCG GCCAGGTGTG CAGCCTATAG CCCTGATGGG 
GAGATGGTGG CCATTGGCAT GAAGAATGGA GAGTTTGTCA TCTTGCTGGT GAACACCCTG 
AAAGTTTGGG GGAAAAAACG AGACCGGAAA TCTGCCATCC AAGATATCAG AATCAGCCCA 
GACAACAGAT TCTTAGCTGT GGGGTCTTCT GAGCAGACAG TTGACTTCTA TGACCTTACT 
CAGGGCACAA GTCTGAACCG GATCGGCTAC TGCAAAGACA TCCCAAGCTT TGTCATTCAG 
ATGGACTTTT CTGCAGACAG CAAATACATT CAGGTGTCAA CAGGAGCCTA TAAGCGCCAA 
GTACATGAAG TTCCCCTGGG GAAGCAAGTA ACTGAAGCCA TGGTTGTTGA GAAGATCACC 
TGGGCTTCCT GGACAAGTGT TCTCGGAGAT GAAGTCATTG GAATCTGGCC ACGAAACGCA 
GACAAGGCTG ATGTCAACTG TGCATGTGTG ACCCATGCTG GCTTGAACAT TGTCACAGGG 
GATGACTTCG GGCTGCTGAA GCTCTTTGAT TTTCCATGCA CAGAAAAATT CGCCAAGCAC 
AAACGTTACT TTGGTCACTC TGCTCATGTG ACGAACATCC GCTTCTCTTC CGATGACAAG 
TATGTGGTCA GCACAGGAGG AGACGACTGC AGTGTATTTG TGTGGCGATG TCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666128.2
CDS	669..6545
Translation	MADRTAPRCQLRLEWVYGYRGHQCRNNLYYTAGKEVVYFVAGVGVVYNTREHSQKFFLGHNDDIISLALHPDKTLIATGQVGKEPYICIWNSYNVHTVSILKDVHTHGVACLAFDSDGQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPSGKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSADGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	NM_146016.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146016.2

ORF Insert Sequence:

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGCTGACG GAAAATGTCT CGTGTCGGTT GGTTTAGACG ATTTTCACAG TGTTGTGTTT 
TGGGACTGGA AAAAGGGAGA AAAGATAGCC ACAACCAGGG GACACAAAGA CAAAATATTT 
GTGGTAAAGT GTAATCCACA GCATGCTGAC AAGTTGGTGA CAGTTGGGAT TAAGCATATC 
AAGTTCTGGC AACAAGCAGG TGGGGGCTTC ACCTCTAAAA GAGGAAGTTT TGGAAGTGCT 
GGAAAGTTGG AAACAATGAT GTGTGTTTCT TATGGGCGAA TGGAAGATCT GGTGTTCTCA 
GGTGCAGCCA CTGGAGATAT TTTTATCTGG AAGGACGTTC TGCTACTGAA GACAGTGAAG 
GCTCATGATG GGCCTGTGTT TGCCATGTAT GCATTAGACA AGGGCTTTGT AACAGGTGGA 
AAGGATGGAA TTGTGGAACT CTGGGATGAT ATGTTTGAAA GATGCTTGAA GACTTATGCC 
ATCAAAAGGA CTGCATTGTC AACCAGCTCA AAAGGACTGC TTTTGGAGGA TAACCCTTCA 
ATTCGTGCAA TTACCCTAGG GCATGGACAC ATTCTCGTGG GGACAAAAAA TGGAGAGATT 
CTAGAAATTG ATAAGAGTGG CCCGATGACC CTGCTTGTTC AGGGACACAT GGAAGGGGAA 
GTGTGGGGGC TGGCAGCACA CCCGCTCCTG CCCATCTGCG CGACAGTAAG TGATGACAAA 
ACACTTCGGA TCTGGGAATT ATCCTCCCAG CACCGCATGC TGGCTGTACG GAAACTTAAA 
AAAGGAGGAA GATGTTGTGC TTTCTCCCCT GATGGGAAAG CCCTAGCAGT TGGATTGAAT 
GATGGAAGTT TCTTGGTGGT GAATGCTGAC ACAGTGGAAG ACATGCTCTC CTTCCATCAC 
AGAAAAGAAA TGATCTCTGA TATAAAATTT TCAAAAGATA CAGGAAAATA CCTTGCTGTG 
GCATCTCATG ATAACTTCGT GGATATCTAC AACGTGCTTA CAAGCAAGAG GGTTGGCATC 
TGTAAAGGAG CTTCTAGTTA CATCACACAC ATTGACTGGG ACTCTAGAGG GAAATTACTG 
CAAGTTAATT CAGGTGCCAA AGAACAGCTT TTCTTTGAAG CTCCAAGAGG CAGAAAACAC 
ACAATAAGGC CATCAGAGGC TGAGAAGATA GAATGGGACA CCTGGACCTG TGTCCTGGGG 
CCCACCTGTG AAGGAATCTG GCCTGCTCAC AGCGATGTGA CTGACGTCAA TGCTGCCAAT 
CTTACCAAAG ATGGCTCCCT CTTAGCAACT GGGGATGACT TTGGTTTTGT GAAGCTTTTT 
TCATATCCTG TCAAGGGACA GCATGCAAGA TTTAAGAAAT ATGTGGGGCA CAGCGCACAC 
GTTACCAATG TGAGGTGGCT ACACAATGAC TCTGTGCTGC TCACTGTAGG AGGTGCTGAC 
ACAGCCTTGA TGATCTGGAC CAGGGAATTT GTGGGGACGC AGGAGAGCAA GCTGGTTGAT 
AGTGAGGAGT CAGACACAGA TGCAGAAGAA GATGGAGGCT ATGACAGTGA TGTCGCTAGA 
GAGAAAGCCA TTGACTATAC CACCAAGATC TATGCTGTGA GCATCAGGGA AATGGAAGGC 
ACCAAACCAC ACCAGCAGCT AAAAGAGGTG TCCATGGAAG AAAGACCACC CGTTAGCCGT 
GCAGCCCCCC AGCCCGAGAA ACTCCAGAAG AACAACATCA CCAAGAAAAA GAAGCTGGTT 
GAGGAGCTGG CTCTGGACCA CGTGTTTGGC TACCGAGGAT TTGACTGCCG CAACAACCTG 
CATTACCTTA ACGATGGTGC CGACATCATT TTCCACACTG CGGCAGCTGG CATTGTGCAG 
AACCTCTCCA CAGGCAGCCA GAGCTTCTAC CTGGAGCACA CGGATGACAT CCTCTGCCTC 
ACAGTGAATC AGCACCCAAA GTACAGAAAT GTGGTGGCTA CCAGCCAGAT AGGGACAACA 
CCTTCAATTC ATATCTGGGA TGCAATGACC AAACACACCC TTTCCATGCT GCGGTGCTTC 
CACACCAAGG GGGTGAATTA CATCAACTTC AGTGCAACCG GGAAGCTCCT GGTGTCTGTG 
GGCGTGGATC CAGAGCACAC CATCACTGTC TGGAGATGGC AAGAAGGTAC CAAGGTTGCC 
AGCCGAGGGG GCCACCTGGA ACGAATATTT GTGGTGGAAT TTCGCCCTGA CTCAGACACA 
CAATTTGTAT CTGTTGGAGT CAAACATATG AAGTTCTGGA CTCTGGCAGG AAGTGCTCTG 
CTGTACAAGA AAGGGGTCAT CGGGTCTATG GAAGCTGCCA AGATGCAAAC GATGCTGTCC 
GTGGCTTTTG GTGCTAACAA CCTTACTTTT ACTGGTGCCA TCAATGGAGA TGTCTATGTC 
TGGAAGGAGC ACTTCCTCAT CCGGCTGGTG GCCAAGGCTC ACACAGGTCC TGTCTTTACA 
ATGTACACCA CCCTCCGAGA TGGGCTTATT GTGACTGGCG GAAAAGAACG ACCGACTAAG 
GAAGGAGGAG CTGTAAAGTT GTGGGACCAG GAGATGAAGC GCTGTCGGGC CTTCCAGCTG 
GAAACAGGGC AGCTGGTGGA ATGTGTGCGC TCGGTGTGCC GTGGCAAGGG AAAAATCTTA 
GTAGGAACCA AAGATGGTGA AATAATTGAA GTTGGGGAAA AAAGCGCTGC TTCAAACATC 
CTGATTGATG GACACATGGA GGGGGAGATC TGGGGCCTAG CCACACACCC CTCCAAGGAC 
ATGTTCATCT CAGCCAGCAA TGATGGCACA GCAAGGATCT GGGACCTGGC TGACAAGAAA 
CTGTTAAACA AGGTGAACCT GGGCCATGCG GCCAGGTGTG CAGCCTATAG CCCTGATGGG 
GAGATGGTGG CCATTGGCAT GAAGAATGGA GAGTTTGTCA TCTTGCTGGT GAACACCCTG 
AAAGTTTGGG GGAAAAAACG AGACCGGAAA TCTGCCATCC AAGATATCAG AATCAGCCCA 
GACAACAGAT TCTTAGCTGT GGGGTCTTCT GAGCAGACAG TTGACTTCTA TGACCTTACT 
CAGGGCACAA GTCTGAACCG GATCGGCTAC TGCAAAGACA TCCCAAGCTT TGTCATTCAG 
ATGGACTTTT CTGCAGACAG CAAATACATT CAGGTGTCAA CAGGAGCCTA TAAGCGCCAA 
GTACATGAAG TTCCCCTGGG GAAGCAAGTA ACTGAAGCCA TGGTTGTTGA GAAGATCACC 
TGGGCTTCCT GGACAAGTGT TCTCGGAGAT GAAGTCATTG GAATCTGGCC ACGAAACGCA 
GACAAGGCTG ATGTCAACTG TGCATGTGTG ACCCATGCTG GCTTGAACAT TGTCACAGGG 
GATGACTTCG GGCTGCTGAA GCTCTTTGAT TTTCCATGCA CAGAAAAATT CGCCAAGCAC 
AAACGTTACT TTGGTCACTC TGCTCATGTG ACGAACATCC GCTTCTCTTC CGATGACAAG 
TATGTGGTCA GCACAGGAGG AGACGACTGC AGTGTATTTG TGTGGCGATG TCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml6 ( XM_006514694.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514757.1 Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X1, mRNA.

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CloneID	OMu45206
Clone ID Related Accession (Same CDS sequence)	XM_006514694.3 , XM_006514694.4
Accession Version	XM_006514694.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	echinoderm microtubule-associated protein-like 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514694.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGGGATTC GTGTGGAGAA AAAGACAAGT AGGTTGACAT CAAATGTGGG ACTACAAGAG 
ATTTGGAAGA ACAGATCTGA CGGAAAATGT CTCGTGTCGG TTGGTTTAGA CGATTTTCAC 
AGTGTTGTGT TTTGGGACTG GAAAAAGGGA GAAAAGATAG CCACAACCAG GGGACACAAA 
GACAAAATAT TTGTGGTAAA GTGTAATCCA CAGCATGCTG ACAAGTTGGT GACAGTTGGG 
ATTAAGCATA TCAAGTTCTG GCAACAAGCA GGTGGGGGCT TCACCTCTAA AAGAGGAAGT 
TTTGGAAGTG CTGGAAAGTT GGAAACAATG ATGTGTGTTT CTTATGGGCG AATGGAAGAT 
CTGGTGTTCT CAGGTGCAGC CACTGGAGAT ATTTTTATCT GGAAGGACGT TCTGCTACTG 
AAGACAGTGA AGGCTCATGA TGGGCCTGTG TTTGCCATGT ATGCATTAGA CAAGGGCTTT 
GTAACAGGTG GAAAGGATGG AATTGTGGAA CTCTGGGATG ATATGTTTGA AAGATGCTTG 
AAGACTTATG CCATCAAAAG GACTGCATTG TCAACCAGCT CAAAAGGACT GCTTTTGGAG 
GATAACCCTT CAATTCGTGC AATTACCCTA GGGCATGGAC ACATTCTCGT GGGGACAAAA 
AATGGAGAGA TTCTAGAAAT TGATAAGAGT GGCCCGATGA CCCTGCTTGT TCAGGGACAC 
ATGGAAGGGG AAGTGTGGGG GCTGGCAGCA CACCCGCTCC TGCCCATCTG CGCGACAGTA 
AGTGATGACA AAACACTTCG GATCTGGGAA TTATCCTCCC AGCACCGCAT GCTGGCTGTA 
CGGAAACTTA AAAAAGGAGG AAGATGTTGT GCTTTCTCCC CTGATGGGAA AGCCCTAGCA 
GTTGGATTGA ATGATGGAAG TTTCTTGGTG GTGAATGCTG ACACAGTGGA AGACATGCTC 
TCCTTCCATC ACAGAAAAGA AATGATCTCT GATATAAAAT TTTCAAAAGA TACAGGAAAA 
TACCTTGCTG TGGCATCTCA TGATAACTTC GTGGATATCT ACAACGTGCT TACAAGCAAG 
AGGGTTGGCA TCTGTAAAGG AGCTTCTAGT TACATCACAC ACATTGACTG GGACTCTAGA 
GGGAAATTAC TGCAAGTTAA TTCAGGTGCC AAAGAACAGC TTTTCTTTGA AGCTCCAAGA 
GGCAGAAAAC ACACAATAAG GCCATCAGAG GCTGAGAAGA TAGAATGGGA CACCTGGACC 
TGTGTCCTGG GGCCCACCTG TGAAGGAATC TGGCCTGCTC ACAGCGATGT GACTGACGTC 
AATGCTGCCA ATCTTACCAA AGATGGCTCC CTCTTAGCAA CTGGGGATGA CTTTGGTTTT 
GTGAAGCTTT TTTCATATCC TGTCAAGGGA CAGCATGCAA GATTTAAGAA ATATGTGGGG 
CACAGCGCAC ACGTTACCAA TGTGAGGTGG CTACACAATG ACTCTGTGCT GCTCACTGTA 
GGAGGTGCTG ACACAGCCTT GATGATCTGG ACCAGGGAAT TTGTGGGGAC GCAGGAGAGC 
AAGCTGGTTG ATAGTGAGGA GTCAGACACA GATGCAGAAG AAGATGGAGG CTATGACAGT 
GATGTCGCTA GAGAGAAAGC CATTGACTAT ACCACCAAGA TCTATGCTGT GAGCATCAGG 
GAAATGGAAG GCACCAAACC ACACCAGCAG CTAAAAGAGG TGTCCATGGA AGAAAGGCAG 
GGAATTGTCA AGGGATCCAG ACCACCCGTT AGCCGTGCAG CCCCCCAGCC CGAGAAACTC 
CAGAAGAACA ACATCACCAA GAAAAAGAAG CTGGTTGAGG AGCTGGCTCT GGACCACGTG 
TTTGGCTACC GAGGATTTGA CTGCCGCAAC AACCTGCATT ACCTTAACGA TGGTGCCGAC 
ATCATTTTCC ACACTGCGGC AGCTGGCATT GTGCAGAACC TCTCCACAGG CAGCCAGAGC 
TTCTACCTGG AGCACACGGA TGACATCCTC TGCCTCACAG TGAATCAGCA CCCAAAGTAC 
AGAAATGTGG TGGCTACCAG CCAGATAGGG ACAACACCTT CAATTCATAT CTGGGATGCA 
ATGACCAAAC ACACCCTTTC CATGCTGCGG TGCTTCCACA CCAAGGGGGT GAATTACATC 
AACTTCAGTG CAACCGGGAA GCTCCTGGTG TCTGTGGGCG TGGATCCAGA GCACACCATC 
ACTGTCTGGA GATGGCAAGA AGGTACCAAG GTTGCCAGCC GAGGGGGCCA CCTGGAACGA 
ATATTTGTGG TGGAATTTCG CCCTGACTCA GACACACAAT TTGTATCTGT TGGAGTCAAA 
CATATGAAGT TCTGGACTCT GGCAGGAAGT GCTCTGCTGT ACAAGAAAGG GGTCATCGGG 
TCTATGGAAG CTGCCAAGAT GCAAACGATG CTGTCCGTGG CTTTTGGTGC TAACAACCTT 
ACTTTTACTG GTGCCATCAA TGGAGATGTC TATGTCTGGA AGGAGCACTT CCTCATCCGG 
CTGGTGGCCA AGGCTCACAC AGGTCCTGTC TTTACAATGT ACACCACCCT CCGAGATGGG 
CTTATTGTGA CTGGCGGAAA AGAACGACCG ACTAAGGAAG GAGGAGCTGT AAAGTTGTGG 
GACCAGGAGA TGAAGCGCTG TCGGGCCTTC CAGCTGGAAA CAGGGCAGCT GGTGGAATGT 
GTGCGCTCGG TGTGCCGTGG CAAGGGAAAA ATCTTAGTAG GAACCAAAGA TGGTGAAATA 
ATTGAAGTTG GGGAAAAAAG CGCTGCTTCA AACATCCTGA TTGATGGACA CATGGAGGGG 
GAGATCTGGG GCCTAGCCAC ACACCCCTCC AAGGACATGT TCATCTCAGC CAGCAATGAT 
GGCACAGCAA GGATCTGGGA CCTGGCTGAC AAGAAACTGT TAAACAAGGT GAACCTGGGC 
CATGCGGCCA GGTGTGCAGC CTATAGCCCT GATGGGGAGA TGGTGGCCAT TGGCATGAAG 
AATGGAGAGT TTGTCATCTT GCTGGTGAAC ACCCTGAAAG TTTGGGGGAA AAAACGAGAC 
CGGAAATCTG CCATCCAAGA TATCAGAATC AGCCCAGACA ACAGATTCTT AGCTGTGGGG 
TCTTCTGAGC AGACAGTTGA CTTCTATGAC CTTACTCAGG GCACAAGTCT GAACCGGATC 
GGCTACTGCA AAGACATCCC AAGCTTTGTC ATTCAGATGG ACTTTTCTGC AGACAGCAAA 
TACATTCAGG TGTCAACAGG AGCCTATAAG CGCCAAGTAC ATGAAGTTCC CCTGGGGAAG 
CAAGTAACTG AAGCCATGGT TGTTGAGAAG ATCACCTGGG CTTCCTGGAC AAGTGTTCTC 
GGAGATGAAG TCATTGGAAT CTGGCCACGA AACGCAGACA AGGCTGATGT CAACTGTGCA 
TGTGTGACCC ATGCTGGCTT GAACATTGTC ACAGGGGATG ACTTCGGGCT GCTGAAGCTC 
TTTGATTTTC CATGCACAGA AAAATTCGCC AAGCACAAAC GTTACTTTGG TCACTCTGCT 
CATGTGACGA ACATCCGCTT CTCTTCCGAT GACAAGTATG TGGTCAGCAC AGGAGGAGAC 
GACTGCAGTG TATTTGTGTG GCGATGTCTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514757.1
CDS	671..6643
Translation	MADRTAPRCQLRLEWVYGYRGHQCRNNLYYTAGKEVVYFVAGVGVVYNTREHSQKFFLGHNDDIISLALHPDKTLIATGQVGKEPYICIWNSYNVHTVSILKDVHTHGVACLAFDSDGQHLASVGLDAKNTVCIWDWRKGKLLASATGHSDRIFDISWDPYQPNRMVSCGVKHIKFWTLCGNALTAKRGIFGKTGDLQTILCLACAKEDITYSGALNGDIYVWKGLTLVRTIQGAHSAGIFSLYACEEGFATGGRDGCIRLWDTDFKPITKIDLRETEQGYKGLSIRSVCWKADRLLAGTQDSEIFEVIVRERDKPMLILQGHCEGELWALALHPKKPLAVTGSDDRSVRLWSLADHALIARCNMEEAVRSVSFSPDGSQLALGMKDGSFIVLRVRDMTEVVHIKDRKEVIHEMKFSPDGSYLAVGSNDGPVDVYAVAQRYKKIGECNKSLSFITHIDWSLDSKYLQTNDGAGERLFYKMPSGKPLTSKEEIKGIPWASWTCVRGPEVSGIWPKYTEVIDINSVDANYNSSVLVSGDDFGLVKLFKFPCLKKGAKFRKYVGHSAHVTNVRWSHDFQWVLSTGGADHSVFQWRFIPEAVSNGVLETTPQEGGADSYSEESDSDFSDVPELDSDIEQETQINYDRQVYKEDLPQLKQQSKEKNHAVPFLKREKAPEDSLKLQFIHGYRGYDCRNNLFYTQAGEVVYHIAAVAVVYNRQQHAQRLYLGHDDDILSLTIHPVKDYVATGQVGRDAAVHVWDTQTLKCLSLLKGHHQRGVCALDFSGIRVEKKTSRLTSNVGLQEIWKNRSDGKCLVSVGLDDFHSVVFWDWKKGEKIATTRGHKDKIFVVKCNPQHADKLVTVGIKHIKFWQQAGGGFTSKRGSFGSAGKLETMMCVSYGRMEDLVFSGAATGDIFIWKDVLLLKTVKAHDGPVFAMYALDKGFVTGGKDGIVELWDDMFERCLKTYAIKRTALSTSSKGLLLEDNPSIRAITLGHGHILVGTKNGEILEIDKSGPMTLLVQGHMEGEVWGLAAHPLLPICATVSDDKTLRIWELSSQHRMLAVRKLKKGGRCCAFSPDGKALAVGLNDGSFLVVNADTVEDMLSFHHRKEMISDIKFSKDTGKYLAVASHDNFVDIYNVLTSKRVGICKGASSYITHIDWDSRGKLLQVNSGAKEQLFFEAPRGRKHTIRPSEAEKIEWDTWTCVLGPTCEGIWPAHSDVTDVNAANLTKDGSLLATGDDFGFVKLFSYPVKGQHARFKKYVGHSAHVTNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDAEEDGGYDSDVAREKAIDYTTKIYAVSIREMEGTKPHQQLKEVSMEERQGIVKGSRPPVSRAAPQPEKLQKNNITKKKKLVEELALDHVFGYRGFDCRNNLHYLNDGADIIFHTAAAGIVQNLSTGSQSFYLEHTDDILCLTVNQHPKYRNVVATSQIGTTPSIHIWDAMTKHTLSMLRCFHTKGVNYINFSATGKLLVSVGVDPEHTITVWRWQEGTKVASRGGHLERIFVVEFRPDSDTQFVSVGVKHMKFWTLAGSALLYKKGVIGSMEAAKMQTMLSVAFGANNLTFTGAINGDVYVWKEHFLIRLVAKAHTGPVFTMYTTLRDGLIVTGGKERPTKEGGAVKLWDQEMKRCRAFQLETGQLVECVRSVCRGKGKILVGTKDGEIIEVGEKSAASNILIDGHMEGEIWGLATHPSKDMFISASNDGTARIWDLADKKLLNKVNLGHAARCAAYSPDGEMVAIGMKNGEFVILLVNTLKVWGKKRDRKSAIQDIRISPDNRFLAVGSSEQTVDFYDLTQGTSLNRIGYCKDIPSFVIQMDFSADSKYIQVSTGAYKRQVHEVPLGKQVTEAMVVEKITWASWTSVLGDEVIGIWPRNADKADVNCACVTHAGLNIVTGDDFGLLKLFDFPCTEKFAKHKRYFGHSAHVTNIRFSSDDKYVVSTGGDDCSVFVWRCL

Target ORF information:

RefSeq Version	XM_006514694.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus echinoderm microtubule associated protein like 6 (Eml6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514694.3

ORF Insert Sequence:

ATGGCGGATC GGACAGCTCC CAGATGCCAG CTCCGGCTGG AGTGGGTGTA CGGGTACCGG 
GGTCACCAGT GCCGCAACAA CCTGTACTAC ACCGCCGGCA AGGAGGTGGT CTACTTTGTA 
GCTGGGGTAG GGGTGGTGTA CAACACCCGA GAGCACAGCC AAAAATTCTT CCTGGGACAC 
AACGACGACA TTATCAGCCT TGCCTTACAC CCAGATAAAA CTCTGATTGC AACTGGCCAG 
GTTGGGAAGG AGCCATATAT ATGCATATGG AATTCCTATA ATGTCCATAC TGTGTCTATT 
CTTAAAGATG TCCATACACA CGGAGTTGCA TGCCTGGCTT TTGACTCGGA TGGACAGCAT 
TTAGCCTCTG TGGGATTAGA TGCCAAAAAC ACAGTCTGCA TTTGGGACTG GAGAAAGGGA 
AAACTTCTGG CCTCAGCTAC TGGCCATTCT GACCGGATTT TTGATATTTC CTGGGATCCA 
TATCAGCCAA ACAGAATGGT TAGCTGCGGA GTGAAGCATA TAAAGTTTTG GACACTATGT 
GGAAATGCCC TGACTGCAAA AAGAGGCATA TTTGGCAAAA CAGGAGATCT TCAGACTATC 
CTTTGCCTTG CATGTGCCAA AGAAGACATC ACTTACTCTG GTGCTTTAAA CGGTGACATC 
TATGTCTGGA AAGGGCTCAC CTTAGTCCGC ACTATTCAAG GAGCACATAG TGCTGGGATC 
TTCAGCCTGT ATGCTTGTGA AGAAGGCTTT GCTACTGGTG GGCGAGATGG TTGCATACGA 
TTATGGGACA CTGATTTCAA ACCAATAACC AAAATTGATC TCAGGGAGAC GGAACAAGGA 
TATAAAGGCC TCTCTATTCG GAGTGTGTGC TGGAAAGCTG ACCGCCTTCT AGCAGGAACC 
CAGGACAGTG AGATATTTGA AGTCATTGTT CGAGAGCGAG ATAAGCCAAT GCTCATTCTT 
CAGGGCCACT GCGAGGGGGA ACTGTGGGCT CTGGCATTGC ACCCCAAGAA ACCTCTGGCT 
GTGACAGGCA GTGATGACCG CTCTGTCAGA CTGTGGAGTC TTGCTGACCA TGCCTTGATT 
GCCCGCTGCA ATATGGAGGA AGCAGTTCGT AGTGTATCCT TCAGCCCAGA TGGATCTCAG 
TTGGCCCTAG GCATGAAGGA CGGCTCTTTC ATTGTCCTCC GAGTCAGAGA TATGACAGAA 
GTCGTTCATA TCAAGGACAG GAAAGAAGTC ATTCATGAGA TGAAATTCTC CCCAGATGGC 
TCTTACCTCG CAGTAGGATC CAATGATGGC CCTGTAGATG TCTACGCTGT TGCCCAGCGC 
TATAAGAAAA TAGGAGAATG CAACAAGTCC CTGAGTTTCA TCACGCACAT CGACTGGTCT 
CTGGATAGTA AATACTTGCA AACCAACGAT GGTGCTGGGG AGAGACTGTT CTACAAAATG 
CCATCAGGGA AGCCTTTAAC AAGTAAAGAA GAAATCAAGG GGATTCCCTG GGCCTCCTGG 
ACGTGTGTGA GAGGCCCTGA AGTGAGTGGA ATTTGGCCCA AATACACTGA GGTTATTGAC 
ATCAATTCAG TGGACGCCAA TTATAACAGT TCAGTGTTGG TTTCTGGAGA TGATTTTGGA 
CTGGTCAAAT TGTTCAAATT TCCTTGTCTC AAAAAAGGAG CCAAATTTAG AAAGTATGTG 
GGCCACTCTG CACATGTCAC CAACGTCCGT TGGTCCCACG ATTTCCAGTG GGTGTTAAGC 
ACAGGAGGAG CTGATCATTC TGTTTTCCAG TGGAGATTTA TCCCAGAAGC AGTCAGCAAT 
GGCGTGTTGG AAACCACACC CCAGGAAGGT GGTGCCGACT CCTATAGTGA GGAATCTGAC 
TCTGACTTCT CTGACGTTCC GGAACTGGAC TCTGATATTG AGCAGGAAAC TCAGATCAAT 
TACGATCGCC AGGTTTACAA AGAAGATCTA CCTCAGCTAA AGCAACAAAG TAAAGAGAAA 
AACCATGCAG TGCCATTCCT GAAGAGGGAG AAGGCTCCTG AGGATAGCCT GAAACTGCAG 
TTCATACATG GTTACAGAGG CTATGACTGT AGAAACAATC TGTTCTACAC ACAAGCTGGA 
GAAGTGGTGT ACCACATTGC TGCAGTTGCT GTGGTATACA ACCGGCAACA GCACGCCCAG 
AGGTTATACC TGGGGCACGA TGATGACATT CTCAGCCTGA CCATCCATCC GGTGAAGGAC 
TATGTAGCCA CTGGACAGGT TGGAAGAGAT GCTGCTGTTC ATGTGTGGGA CACTCAGACT 
CTAAAATGTT TGTCATTACT GAAAGGGCAC CATCAGAGAG GAGTGTGTGC TCTGGACTTT 
TCAGGGATTC GTGTGGAGAA AAAGACAAGT AGGTTGACAT CAAATGTGGG ACTACAAGAG 
ATTTGGAAGA ACAGATCTGA CGGAAAATGT CTCGTGTCGG TTGGTTTAGA CGATTTTCAC 
AGTGTTGTGT TTTGGGACTG GAAAAAGGGA GAAAAGATAG CCACAACCAG GGGACACAAA 
GACAAAATAT TTGTGGTAAA GTGTAATCCA CAGCATGCTG ACAAGTTGGT GACAGTTGGG 
ATTAAGCATA TCAAGTTCTG GCAACAAGCA GGTGGGGGCT TCACCTCTAA AAGAGGAAGT 
TTTGGAAGTG CTGGAAAGTT GGAAACAATG ATGTGTGTTT CTTATGGGCG AATGGAAGAT 
CTGGTGTTCT CAGGTGCAGC CACTGGAGAT ATTTTTATCT GGAAGGACGT TCTGCTACTG 
AAGACAGTGA AGGCTCATGA TGGGCCTGTG TTTGCCATGT ATGCATTAGA CAAGGGCTTT 
GTAACAGGTG GAAAGGATGG AATTGTGGAA CTCTGGGATG ATATGTTTGA AAGATGCTTG 
AAGACTTATG CCATCAAAAG GACTGCATTG TCAACCAGCT CAAAAGGACT GCTTTTGGAG 
GATAACCCTT CAATTCGTGC AATTACCCTA GGGCATGGAC ACATTCTCGT GGGGACAAAA 
AATGGAGAGA TTCTAGAAAT TGATAAGAGT GGCCCGATGA CCCTGCTTGT TCAGGGACAC 
ATGGAAGGGG AAGTGTGGGG GCTGGCAGCA CACCCGCTCC TGCCCATCTG CGCGACAGTA 
AGTGATGACA AAACACTTCG GATCTGGGAA TTATCCTCCC AGCACCGCAT GCTGGCTGTA 
CGGAAACTTA AAAAAGGAGG AAGATGTTGT GCTTTCTCCC CTGATGGGAA AGCCCTAGCA 
GTTGGATTGA ATGATGGAAG TTTCTTGGTG GTGAATGCTG ACACAGTGGA AGACATGCTC 
TCCTTCCATC ACAGAAAAGA AATGATCTCT GATATAAAAT TTTCAAAAGA TACAGGAAAA 
TACCTTGCTG TGGCATCTCA TGATAACTTC GTGGATATCT ACAACGTGCT TACAAGCAAG 
AGGGTTGGCA TCTGTAAAGG AGCTTCTAGT TACATCACAC ACATTGACTG GGACTCTAGA 
GGGAAATTAC TGCAAGTTAA TTCAGGTGCC AAAGAACAGC TTTTCTTTGA AGCTCCAAGA 
GGCAGAAAAC ACACAATAAG GCCATCAGAG GCTGAGAAGA TAGAATGGGA CACCTGGACC 
TGTGTCCTGG GGCCCACCTG TGAAGGAATC TGGCCTGCTC ACAGCGATGT GACTGACGTC 
AATGCTGCCA ATCTTACCAA AGATGGCTCC CTCTTAGCAA CTGGGGATGA CTTTGGTTTT 
GTGAAGCTTT TTTCATATCC TGTCAAGGGA CAGCATGCAA GATTTAAGAA ATATGTGGGG 
CACAGCGCAC ACGTTACCAA TGTGAGGTGG CTACACAATG ACTCTGTGCT GCTCACTGTA 
GGAGGTGCTG ACACAGCCTT GATGATCTGG ACCAGGGAAT TTGTGGGGAC GCAGGAGAGC 
AAGCTGGTTG ATAGTGAGGA GTCAGACACA GATGCAGAAG AAGATGGAGG CTATGACAGT 
GATGTCGCTA GAGAGAAAGC CATTGACTAT ACCACCAAGA TCTATGCTGT GAGCATCAGG 
GAAATGGAAG GCACCAAACC ACACCAGCAG CTAAAAGAGG TGTCCATGGA AGAAAGGCAG 
GGAATTGTCA AGGGATCCAG ACCACCCGTT AGCCGTGCAG CCCCCCAGCC CGAGAAACTC 
CAGAAGAACA ACATCACCAA GAAAAAGAAG CTGGTTGAGG AGCTGGCTCT GGACCACGTG 
TTTGGCTACC GAGGATTTGA CTGCCGCAAC AACCTGCATT ACCTTAACGA TGGTGCCGAC 
ATCATTTTCC ACACTGCGGC AGCTGGCATT GTGCAGAACC TCTCCACAGG CAGCCAGAGC 
TTCTACCTGG AGCACACGGA TGACATCCTC TGCCTCACAG TGAATCAGCA CCCAAAGTAC 
AGAAATGTGG TGGCTACCAG CCAGATAGGG ACAACACCTT CAATTCATAT CTGGGATGCA 
ATGACCAAAC ACACCCTTTC CATGCTGCGG TGCTTCCACA CCAAGGGGGT GAATTACATC 
AACTTCAGTG CAACCGGGAA GCTCCTGGTG TCTGTGGGCG TGGATCCAGA GCACACCATC 
ACTGTCTGGA GATGGCAAGA AGGTACCAAG GTTGCCAGCC GAGGGGGCCA CCTGGAACGA 
ATATTTGTGG TGGAATTTCG CCCTGACTCA GACACACAAT TTGTATCTGT TGGAGTCAAA 
CATATGAAGT TCTGGACTCT GGCAGGAAGT GCTCTGCTGT ACAAGAAAGG GGTCATCGGG 
TCTATGGAAG CTGCCAAGAT GCAAACGATG CTGTCCGTGG CTTTTGGTGC TAACAACCTT 
ACTTTTACTG GTGCCATCAA TGGAGATGTC TATGTCTGGA AGGAGCACTT CCTCATCCGG 
CTGGTGGCCA AGGCTCACAC AGGTCCTGTC TTTACAATGT ACACCACCCT CCGAGATGGG 
CTTATTGTGA CTGGCGGAAA AGAACGACCG ACTAAGGAAG GAGGAGCTGT AAAGTTGTGG 
GACCAGGAGA TGAAGCGCTG TCGGGCCTTC CAGCTGGAAA CAGGGCAGCT GGTGGAATGT 
GTGCGCTCGG TGTGCCGTGG CAAGGGAAAA ATCTTAGTAG GAACCAAAGA TGGTGAAATA 
ATTGAAGTTG GGGAAAAAAG CGCTGCTTCA AACATCCTGA TTGATGGACA CATGGAGGGG 
GAGATCTGGG GCCTAGCCAC ACACCCCTCC AAGGACATGT TCATCTCAGC CAGCAATGAT 
GGCACAGCAA GGATCTGGGA CCTGGCTGAC AAGAAACTGT TAAACAAGGT GAACCTGGGC 
CATGCGGCCA GGTGTGCAGC CTATAGCCCT GATGGGGAGA TGGTGGCCAT TGGCATGAAG 
AATGGAGAGT TTGTCATCTT GCTGGTGAAC ACCCTGAAAG TTTGGGGGAA AAAACGAGAC 
CGGAAATCTG CCATCCAAGA TATCAGAATC AGCCCAGACA ACAGATTCTT AGCTGTGGGG 
TCTTCTGAGC AGACAGTTGA CTTCTATGAC CTTACTCAGG GCACAAGTCT GAACCGGATC 
GGCTACTGCA AAGACATCCC AAGCTTTGTC ATTCAGATGG ACTTTTCTGC AGACAGCAAA 
TACATTCAGG TGTCAACAGG AGCCTATAAG CGCCAAGTAC ATGAAGTTCC CCTGGGGAAG 
CAAGTAACTG AAGCCATGGT TGTTGAGAAG ATCACCTGGG CTTCCTGGAC AAGTGTTCTC 
GGAGATGAAG TCATTGGAAT CTGGCCACGA AACGCAGACA AGGCTGATGT CAACTGTGCA 
TGTGTGACCC ATGCTGGCTT GAACATTGTC ACAGGGGATG ACTTCGGGCT GCTGAAGCTC 
TTTGATTTTC CATGCACAGA AAAATTCGCC AAGCACAAAC GTTACTTTGG TCACTCTGCT 
CATGTGACGA ACATCCGCTT CTCTTCCGAT GACAAGTATG TGGTCAGCAC AGGAGGAGAC 
GACTGCAGTG TATTTGTGTG GCGATGTCTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

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