Gene Symbol | Rngtt |
Entrez Gene ID | 24018 |
Full Name | RNA guanylyltransferase and 5'-phosphatase |
Synonyms | AU020997,HCE,MCE1 |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
ORF » Species Summary » Mus musculus » Rngtt cDNA ORF clone
gRNAs
Gene Symbol | Rngtt |
Entrez Gene ID | 24018 |
Full Name | RNA guanylyltransferase and 5'-phosphatase |
Synonyms | AU020997,HCE,MCE1 |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
mRNA | Protein | Name |
---|---|---|
XM_006537889.3 | XP_006537952.1 | mRNA-capping enzyme isoform X2 |
XM_030253517.1 | XP_030109377.1 | mRNA-capping enzyme isoform X5 |
NM_001305273.1 | NP_001292202.1 | mRNA-capping enzyme isoform 2 |
NM_011884.3 | NP_036014.1 | mRNA-capping enzyme isoform 1 |
XM_006537890.3 | XP_006537953.1 | mRNA-capping enzyme isoform X3 |
XM_006537889.4 | XP_006537952.1 | mRNA-capping enzyme isoform X2 |
XM_017320189.2 | XP_017175678.1 | mRNA-capping enzyme isoform X1 |
XM_017320189.1 | XP_017175678.1 | mRNA-capping enzyme isoform X1 |
XM_011250015.2 | XP_011248317.1 | mRNA-capping enzyme isoform X4 |
Canis lupus familiaris (dog) | RNGTT | XP_539035.3 |
Bos taurus (cattle) | RNGTT | NP_001039550.1 |
Mus musculus (house mouse) | Rngtt | NP_036014.1 |
Anopheles gambiae (African malaria mosquito) | AgaP_AGAP005142 | XP_314026.4 |
Caenorhabditis elegans (roundworm) | cel-1 | NP_001020979.1 |
Arabidopsis thaliana (thale cress) | AT3G09100 | NP_974263.1 |
Arabidopsis thaliana (thale cress) | AT5G01290 | NP_195749.2 |
Xenopus tropicalis (tropical clawed frog) | rngtt | NP_001001222.1 |
Pan troglodytes (chimpanzee) | RNGTT | XP_518633.3 |
Arabidopsis thaliana (thale cress) | AT5G28210 | NP_198173.1 |
Homo sapiens (human) | RNGTT | NP_003791.3 |
Danio rerio (zebrafish) | rngtt | NP_998032.1 |
Oryza sativa (rice) | Os12g0193200 | NP_001066345.1 |
Rattus norvegicus (Norway rat) | Rngtt | NP_001101393.1 |
Gallus gallus (chicken) | RNGTT | XP_419843.2 |
Drosophila melanogaster (fruit fly) | mRNA-cap | NP_572952.1 |
The cytoplasmic capping complex assembles on adapter protein nck1 bound to the proline-rich C-terminus of Mammalian capping enzyme.
Mukherjee C, Bakthavachalu B, Schoenberg DR
PLoS biology12(8)e1001933(2014 Aug)
Functional characterization of the C-terminal domain of mouse capping enzyme.
Liu L
Cell biochemistry and function24(2)95-102(2006 Mar-Apr)
BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)
Structure and mechanism of the RNA triphosphatase component of mammalian mRNA capping enzyme.
Changela A, Ho CK, Martins A, Shuman S, Mondrag?n A
The EMBO journal20(10)2575-86(2001 May)
Mammalian capping enzyme binds RNA and uses protein tyrosine phosphatase mechanism.
Wen Y, Yue Z, Shatkin AJ
Proceedings of the National Academy of Sciences of the United States of America95(21)12226-31(1998 Oct)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Overall, both in vivo and in vitro studies indicate that the functional domain of Mce1 is between a.a. 211 and 567, and the heptapeptide sequence between 561 and 567 may play an important role in the enzyme activity.
Title: Functional characterization of the C-terminal domain of mouse capping enzyme.
The following Rngtt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rngtt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OMu14849 | |
Clone ID Related Accession (Same CDS sequence) | NM_011884.3 | |
Accession Version | NM_011884.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-05 | |
Organism | Mus musculus(house mouse) | |
Product | mRNA-capping enzyme isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK151463.1, AK002922.1, AK142016.1, AC093925.5, CO042079.1 and BU756706.1. On Mar 7, 2015 this sequence version replaced NM_011884.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoforom (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660811.155541.1, BC043657.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849380, SAMN00849381 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGGCTTACA ACAAGATCCC GCCGCGGTGG CTGAACTGTC CGCGGCGCGG TCAGCCGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_036014.1 |
CDS | 151..1944 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGCTTACA ACAAGATCCC GCCGCGGTGG CTGAACTGTC CGCGGCGCGG TCAGCCGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu69171 | |
Clone ID Related Accession (Same CDS sequence) | NM_001305273.1 | |
Accession Version | NM_001305273.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-04 | |
Organism | Mus musculus(house mouse) | |
Product | mRNA-capping enzyme isoform 2 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK151463.1, AK002922.1, AC093925.5, AK142016.1, CO042079.1 and BU756706.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which causes a frame-shift compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK002922.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849380, SAMN00849381 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGGCTTACA ACAAGATCCC GCCGCGGTGG CTGAACTGTC CGCGGCGCGG TCAGCCGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001292202.1 |
CDS | 151..1896 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGCTTACA ACAAGATCCC GCCGCGGTGG CTGAACTGTC CGCGGCGCGG TCAGCCGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu32908 | |
Clone ID Related Accession (Same CDS sequence) | XM_006537889.3 , XM_006537889.4 | |
Accession Version | XM_006537889.3 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | mRNA-capping enzyme isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537889.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCTTACA ACAAGATCCC GCCGCGGTGG CTGAACTGTC CGCGGCGCGG TCAGCCGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006537952.1 |
CDS | 162..1553 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGCTTACA ACAAGATCCC GCCGCGGTGG CTGAACTGTC CGCGGCGCGG TCAGCCGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu32909 | |
Clone ID Related Accession (Same CDS sequence) | XM_006537890.3 | |
Accession Version | XM_006537890.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | mRNA-capping enzyme isoform X3 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537890.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCTTACA ACAAGATCCC GCCGCGGTGG CTGAACTGTC CGCGGCGCGG TCAGCCGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006537953.1 |
CDS | 162..1535 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGCTTACA ACAAGATCCC GCCGCGGTGG CTGAACTGTC CGCGGCGCGG TCAGCCGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu69170 | |
Clone ID Related Accession (Same CDS sequence) | XM_011250015.2 | |
Accession Version | XM_011250015.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | mRNA-capping enzyme isoform X4 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCTTACA ACAAGATCCC GCCGCGGTGG CTGAACTGTC CGCGGCGCGG TCAGCCGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_011248317.1 |
CDS | 162..1499 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGCTTACA ACAAGATCCC GCCGCGGTGG CTGAACTGTC CGCGGCGCGG TCAGCCGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu32908 | |
Clone ID Related Accession (Same CDS sequence) | XM_006537889.3 , XM_006537889.4 | |
Accession Version | XM_006537889.4 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | mRNA-capping enzyme isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537889.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCTTACA ACAAGATCCC GCCGCGGTGG CTGAACTGTC CGCGGCGCGG TCAGCCGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006537952.1 |
CDS | 162..1553 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGCTTACA ACAAGATCCC GCCGCGGTGG CTGAACTGTC CGCGGCGCGG TCAGCCGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu80972 | |
Clone ID Related Accession (Same CDS sequence) | XM_017320189.2 , XM_017320189.1 | |
Accession Version | XM_017320189.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | mRNA-capping enzyme isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320189.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCTCCCT CTGTCAGTCT CCAACTTATT TTTTTGAGAC AAGGTCCTTC ACTGAACCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_017175678.1 |
CDS | 376..1857 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGTCTCCCT CTGTCAGTCT CCAACTTATT TTTTTGAGAC AAGGTCCTTC ACTGAACCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu80972 | |
Clone ID Related Accession (Same CDS sequence) | XM_017320189.2 , XM_017320189.1 | |
Accession Version | XM_017320189.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | mRNA-capping enzyme isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCTCCCT CTGTCAGTCT CCAACTTATT TTTTTGAGAC AAGGTCCTTC ACTGAACCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_017175678.1 |
CDS | 377..1858 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGTCTCCCT CTGTCAGTCT CCAACTTATT TTTTTGAGAC AAGGTCCTTC ACTGAACCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu88533 | |
Clone ID Related Accession (Same CDS sequence) | XM_030253517.1 | |
Accession Version | XM_030253517.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | mRNA-capping enzyme isoform X5 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCTCCCT CTGTCAGTCT CCAACTTATT TTTTTGAGAC AAGGTCCTTC ACTGAACCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_030109377.1 |
CDS | 376..1683 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGTCTCCCT CTGTCAGTCT CCAACTTATT TTTTTGAGAC AAGGTCCTTC ACTGAACCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The cytoplasmic capping complex assembles on adapter protein nck1 bound to the proline-rich C-terminus of Mammalian capping enzyme. |
Functional characterization of the C-terminal domain of mouse capping enzyme. |
BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. |
Structure and mechanism of the RNA triphosphatase component of mammalian mRNA capping enzyme. |
Mammalian capping enzyme binds RNA and uses protein tyrosine phosphatase mechanism. |