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Srp54a cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Srp54a
Entrez Gene ID 24067
Full Name signal recognition particle 54A
Synonyms 54kDa,Srp54
General protein information
Preferred Names

signal recognition particle 54A

Names

signal recognition particle 54 kDa protein
54K subunit of signal recognition particle
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

12

12|12 C1

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_030246710.1 XP_030102570.1 signal recognition particle 54 kDa protein isoform X1
XM_006515868.3 XP_006515931.1 signal recognition particle 54 kDa protein isoform X1
NM_011899.4 NP_036029.2 signal recognition particle 54 kDa protein

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Srp54a NP_036029.2
Danio rerio (zebrafish) srp54 NP_957282.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004610 XP_315212.2
Caenorhabditis elegans (roundworm) F21D5.7 NP_501507.1
Saccharomyces cerevisiae (baker's yeast) SRP54 NP_015413.1
Kluyveromyces lactis KLLA0D09020g XP_453464.1
Homo sapiens (human) SRP54 NP_003127.1
Rattus norvegicus (Norway rat) Srp54a NP_446323.1
Magnaporthe oryzae (rice blast fungus) MGG_05977 XP_003711822.1
Arabidopsis thaliana (thale cress) AT1G48900 NP_564535.1
Canis lupus familiaris (dog) SRP54 NP_001003272.1
Bos taurus (cattle) SRP54 NP_001033290.1
Gallus gallus (chicken) SRP54 XP_421238.1
Schizosaccharomyces pombe (fission yeast) srp54 NP_588209.1
Neurospora crassa NCU09696 XP_960643.1
Oryza sativa (rice) Os01g0772800 NP_001044395.1
Pan troglodytes (chimpanzee) SRP54 XP_001136048.1
SRP54 XP_001082531.1
Mus musculus (house mouse) Srp54b NP_001093579.1
Drosophila melanogaster (fruit fly) Srp54k NP_523931.1
Eremothecium gossypii AGOS_AAR041W NP_982582.2
Xenopus tropicalis (tropical clawed frog) srp54 NP_988977.1

Gene Ontology


Bibliography

Related articles in PubMed

Structure of the signal recognition particle interacting with the elongation-arrested ribosome.
Halic M, Becker T, Pool MR, Spahn CM, Grassucci RA, Frank J, Beckmann R
Nature427(6977)808-14(2004 Feb)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

The methionine-rich domain of the 54 kd protein subunit of the signal recognition particle contains an RNA binding site and can be crosslinked to a signal sequence.
Zopf D, Bernstein HD, Johnson AE, Walter P
The EMBO journal9(13)4511-7(1990 Dec)

Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle.
Bernstein HD, Poritz MA, Strub K, Hoben PJ, Brenner S, Walter P
Nature340(6233)482-6(1989 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Srp54a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Srp54a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu03443 NM_011899.4
Latest version!		 Mus musculus signal recognition particle 54A (Srp54a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu47842 XM_006515868.3
Latest version!		 Mus musculus signal recognition particle 54A (Srp54a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu47842 XM_030246710.1
Latest version!		 Mus musculus signal recognition particle 54A (Srp54a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu03443
Clone ID Related Accession (Same CDS sequence) NM_011899.4
Accession Version NM_011899.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1515bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-06-07
Organism Mus musculus(house mouse)
Product signal recognition particle 54 kDa protein
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC157515.2. On Jul 25, 2007 this sequence version replaced NM_011899.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC019683.1, AK011928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGTTCTAG CAGATCTTGG AAGAAAAATA ACATCAGCAT TACGCTCATT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTAAATGCT ATGCTGAAAG AAGTATGTAC AGCATTATTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTCAGAG AAAATGTTAA GTCTGCAATT 
GATCTTGAAG AGATGGCATC TGGACTGAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TAAAGCTTGT AGACCCTGGA GTTAAAGCGT GGACACCGAC TAAGGGAAAG 
CAAAATGTGA TCATGTTTGT TGGATTGCAA GGGAGTGGTA AAACGACAAC ATGTTCAAAG 
TTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TGATATGTGC AGATACATTC 
AGAGCAGGAG CCTTTGACCA GCTAAAACAG AATGCCACCA AAGCAAGAAT TCCGTTCTAT 
GGCAGCTATA CTGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTGGA GAAATTCAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGTC GTCACAAACA AGAAGACTCT 
TTATTTGAAG AAATGCTTCA AGTTTCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCAT CCATCGGACA GGCTTGTGAG GCCCAGGCGA AGGCCTTTAA AGACAAAGTA 
GATGTAGCTT CAGTAATAGT GACAAAACTC GACGGTCATG CGAAAGGAGG CGGTGCTCTT 
AGTGCAGTTG CTGCCACAAA AAGTCCAATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GATTTTGAAC CTTTCAAAAC ACAACCTTTC ATCAGCAAAC TCCTTGGAAT GGGTGATATT 
GAAGGACTGA TTGATAAAGT CAATGAATTG AAGTTGGATG ATAATGAGGC ACTTATAGAG 
AAGTTGAAGC ACGGTCAGTT TACATTGCGA GACATGTATG AACAGTTTCA GAATATTATG 
AAAATGGGCC CATTCAGTCA GATATTGGGG ATGATTCCTG GCTTTGGCAC AGATTTTATG 
AGCAAAGGGA ATGAGCAGGA GTCAATGGCA AGGCTGAAGA AACTGATGAC AATCATGGAC 
AGTATGAACG ATCAAGAACT GGACAGTACA GATGGTGCCA AGGTTTTCAG TAAGCAACCA 
GGGAGAATCC AAAGAGTTGC CCGGGGATCA GGTGTGTCAA CAAGAGATGT TCAAGAACTT 
CTGACCCAGT ATACCAAGTT TGCACAGATG GTCAAAAAGA TGGGAGGTAT CAAAGGACTT 
TTCAAAGGCG GTGATATGTC TAAGAATGTG AGTCAGTCAC AGATGGCAAA ATTAAACCAA 
CAAATGGCCA AAATGATGGA CCCACGAGTT CTTCATCACA TGGGTGGCAT GGCCGGCCTT 
CAGTCAATGA TGCGGCAGTT TCAGCAGGGT GCTGCTGGCA ACATGAAAGG CATGATGGGA 
TTCAATAACA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_036029.2
CDS274..1788
Translation

Target ORF information:

RefSeq Version NM_011899.4
Organism Mus musculus(house mouse)
Definition Mus musculus signal recognition particle 54A (Srp54a), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_011899.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGTTCTAG CAGATCTTGG AAGAAAAATA ACATCAGCAT TACGCTCATT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTAAATGCT ATGCTGAAAG AAGTATGTAC AGCATTATTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTCAGAG AAAATGTTAA GTCTGCAATT 
GATCTTGAAG AGATGGCATC TGGACTGAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TAAAGCTTGT AGACCCTGGA GTTAAAGCGT GGACACCGAC TAAGGGAAAG 
CAAAATGTGA TCATGTTTGT TGGATTGCAA GGGAGTGGTA AAACGACAAC ATGTTCAAAG 
TTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TGATATGTGC AGATACATTC 
AGAGCAGGAG CCTTTGACCA GCTAAAACAG AATGCCACCA AAGCAAGAAT TCCGTTCTAT 
GGCAGCTATA CTGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTGGA GAAATTCAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGTC GTCACAAACA AGAAGACTCT 
TTATTTGAAG AAATGCTTCA AGTTTCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCAT CCATCGGACA GGCTTGTGAG GCCCAGGCGA AGGCCTTTAA AGACAAAGTA 
GATGTAGCTT CAGTAATAGT GACAAAACTC GACGGTCATG CGAAAGGAGG CGGTGCTCTT 
AGTGCAGTTG CTGCCACAAA AAGTCCAATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GATTTTGAAC CTTTCAAAAC ACAACCTTTC ATCAGCAAAC TCCTTGGAAT GGGTGATATT 
GAAGGACTGA TTGATAAAGT CAATGAATTG AAGTTGGATG ATAATGAGGC ACTTATAGAG 
AAGTTGAAGC ACGGTCAGTT TACATTGCGA GACATGTATG AACAGTTTCA GAATATTATG 
AAAATGGGCC CATTCAGTCA GATATTGGGG ATGATTCCTG GCTTTGGCAC AGATTTTATG 
AGCAAAGGGA ATGAGCAGGA GTCAATGGCA AGGCTGAAGA AACTGATGAC AATCATGGAC 
AGTATGAACG ATCAAGAACT GGACAGTACA GATGGTGCCA AGGTTTTCAG TAAGCAACCA 
GGGAGAATCC AAAGAGTTGC CCGGGGATCA GGTGTGTCAA CAAGAGATGT TCAAGAACTT 
CTGACCCAGT ATACCAAGTT TGCACAGATG GTCAAAAAGA TGGGAGGTAT CAAAGGACTT 
TTCAAAGGCG GTGATATGTC TAAGAATGTG AGTCAGTCAC AGATGGCAAA ATTAAACCAA 
CAAATGGCCA AAATGATGGA CCCACGAGTT CTTCATCACA TGGGTGGCAT GGCCGGCCTT 
CAGTCAATGA TGCGGCAGTT TCAGCAGGGT GCTGCTGGCA ACATGAAAGG CATGATGGGA 
TTCAATAACA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu47842
Clone ID Related Accession (Same CDS sequence) XM_030246710.1 , XM_006515868.3
Accession Version XM_006515868.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1098bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product signal recognition particle 54 kDa protein isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515868.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTTCAAAG GAGCCTTTGA CCAGCTAAAA CAGAATGCCA CCAAAGCAAG AATTCCGTTC 
TATGGCAGCT ATACTGAAAT GGATCCTGTC ATCATTGCTT CTGAAGGAGT GGAGAAATTC 
AAAAATGAAA ATTTTGAAAT TATTATTGTT GATACAAGTG GTCGTCACAA ACAAGAAGAC 
TCTTTATTTG AAGAAATGCT TCAAGTTTCT AATGCTATAC AACCTGATAA CATTGTTTAT 
GTGATGGATG CATCCATCGG ACAGGCTTGT GAGGCCCAGG CGAAGGCCTT TAAAGACAAA 
GTAGATGTAG CTTCAGTAAT AGTGACAAAA CTCGACGGTC ATGCGAAAGG AGGCGGTGCT 
CTTAGTGCAG TTGCTGCCAC AAAAAGTCCA ATTATTTTCA TTGGTACAGG GGAACATATA 
GATGATTTTG AACCTTTCAA AACACAACCT TTCATCAGCA AACTCCTTGG AATGGGTGAT 
ATTGAAGGAC TGATTGATAA AGTCAATGAA TTGAAGTTGG ATGATAATGA GGCACTTATA 
GAGAAGTTGA AGCACGGTCA GTTTACATTG CGAGACATGT ATGAACAGTT TCAGAATATT 
ATGAAAATGG GCCCATTCAG TCAGATATTG GGGATGATTC CTGGCTTTGG CACAGATTTT 
ATGAGCAAAG GGAATGAGCA GGAGTCAATG GCAAGGCTGA AGAAACTGAT GACAATCATG 
GACAGTATGA ACGATCAAGA ACTGGACAGT ACAGATGGTG CCAAGGTTTT CAGTAAGCAA 
CCAGGGAGAA TCCAAAGAGT TGCCCGGGGA TCAGGTGTGT CAACAAGAGA TGTTCAAGAA 
CTTCTGACCC AGTATACCAA GTTTGCACAG ATGGTCAAAA AGATGGGAGG TATCAAAGGA 
CTTTTCAAAG GCGGTGATAT GTCTAAGAAT GTGAGTCAGT CACAGATGGC AAAATTAAAC 
CAACAAATGG CCAAAATGAT GGACCCACGA GTTCTTCATC ACATGGGTGG CATGGCCGGC 
CTTCAGTCAA TGATGCGGCA GTTTCAGCAG GGTGCTGCTG GCAACATGAA AGGCATGATG 
GGATTCAATA ACATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006515931.1
CDS85..1182
Translation

Target ORF information:

RefSeq Version XM_006515868.3
Organism Mus musculus(house mouse)
Definition Mus musculus signal recognition particle 54A (Srp54a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006515868.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTTCAAAG GAGCCTTTGA CCAGCTAAAA CAGAATGCCA CCAAAGCAAG AATTCCGTTC 
TATGGCAGCT ATACTGAAAT GGATCCTGTC ATCATTGCTT CTGAAGGAGT GGAGAAATTC 
AAAAATGAAA ATTTTGAAAT TATTATTGTT GATACAAGTG GTCGTCACAA ACAAGAAGAC 
TCTTTATTTG AAGAAATGCT TCAAGTTTCT AATGCTATAC AACCTGATAA CATTGTTTAT 
GTGATGGATG CATCCATCGG ACAGGCTTGT GAGGCCCAGG CGAAGGCCTT TAAAGACAAA 
GTAGATGTAG CTTCAGTAAT AGTGACAAAA CTCGACGGTC ATGCGAAAGG AGGCGGTGCT 
CTTAGTGCAG TTGCTGCCAC AAAAAGTCCA ATTATTTTCA TTGGTACAGG GGAACATATA 
GATGATTTTG AACCTTTCAA AACACAACCT TTCATCAGCA AACTCCTTGG AATGGGTGAT 
ATTGAAGGAC TGATTGATAA AGTCAATGAA TTGAAGTTGG ATGATAATGA GGCACTTATA 
GAGAAGTTGA AGCACGGTCA GTTTACATTG CGAGACATGT ATGAACAGTT TCAGAATATT 
ATGAAAATGG GCCCATTCAG TCAGATATTG GGGATGATTC CTGGCTTTGG CACAGATTTT 
ATGAGCAAAG GGAATGAGCA GGAGTCAATG GCAAGGCTGA AGAAACTGAT GACAATCATG 
GACAGTATGA ACGATCAAGA ACTGGACAGT ACAGATGGTG CCAAGGTTTT CAGTAAGCAA 
CCAGGGAGAA TCCAAAGAGT TGCCCGGGGA TCAGGTGTGT CAACAAGAGA TGTTCAAGAA 
CTTCTGACCC AGTATACCAA GTTTGCACAG ATGGTCAAAA AGATGGGAGG TATCAAAGGA 
CTTTTCAAAG GCGGTGATAT GTCTAAGAAT GTGAGTCAGT CACAGATGGC AAAATTAAAC 
CAACAAATGG CCAAAATGAT GGACCCACGA GTTCTTCATC ACATGGGTGG CATGGCCGGC 
CTTCAGTCAA TGATGCGGCA GTTTCAGCAG GGTGCTGCTG GCAACATGAA AGGCATGATG 
GGATTCAATA ACATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu47842
Clone ID Related Accession (Same CDS sequence) XM_030246710.1 , XM_006515868.3
Accession Version XM_030246710.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1098bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product signal recognition particle 54 kDa protein isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTTCAAAG GAGCCTTTGA CCAGCTAAAA CAGAATGCCA CCAAAGCAAG AATTCCGTTC 
TATGGCAGCT ATACTGAAAT GGATCCTGTC ATCATTGCTT CTGAAGGAGT GGAGAAATTC 
AAAAATGAAA ATTTTGAAAT TATTATTGTT GATACAAGTG GTCGTCACAA ACAAGAAGAC 
TCTTTATTTG AAGAAATGCT TCAAGTTTCT AATGCTATAC AACCTGATAA CATTGTTTAT 
GTGATGGATG CATCCATCGG ACAGGCTTGT GAGGCCCAGG CGAAGGCCTT TAAAGACAAA 
GTAGATGTAG CTTCAGTAAT AGTGACAAAA CTCGACGGTC ATGCGAAAGG AGGCGGTGCT 
CTTAGTGCAG TTGCTGCCAC AAAAAGTCCA ATTATTTTCA TTGGTACAGG GGAACATATA 
GATGATTTTG AACCTTTCAA AACACAACCT TTCATCAGCA AACTCCTTGG AATGGGTGAT 
ATTGAAGGAC TGATTGATAA AGTCAATGAA TTGAAGTTGG ATGATAATGA GGCACTTATA 
GAGAAGTTGA AGCACGGTCA GTTTACATTG CGAGACATGT ATGAACAGTT TCAGAATATT 
ATGAAAATGG GCCCATTCAG TCAGATATTG GGGATGATTC CTGGCTTTGG CACAGATTTT 
ATGAGCAAAG GGAATGAGCA GGAGTCAATG GCAAGGCTGA AGAAACTGAT GACAATCATG 
GACAGTATGA ACGATCAAGA ACTGGACAGT ACAGATGGTG CCAAGGTTTT CAGTAAGCAA 
CCAGGGAGAA TCCAAAGAGT TGCCCGGGGA TCAGGTGTGT CAACAAGAGA TGTTCAAGAA 
CTTCTGACCC AGTATACCAA GTTTGCACAG ATGGTCAAAA AGATGGGAGG TATCAAAGGA 
CTTTTCAAAG GCGGTGATAT GTCTAAGAAT GTGAGTCAGT CACAGATGGC AAAATTAAAC 
CAACAAATGG CCAAAATGAT GGACCCACGA GTTCTTCATC ACATGGGTGG CATGGCCGGC 
CTTCAGTCAA TGATGCGGCA GTTTCAGCAG GGTGCTGCTG GCAACATGAA AGGCATGATG 
GGATTCAATA ACATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_030102570.1
CDS644..1741
Translation

Target ORF information:

RefSeq Version XM_030246710.1
Organism Mus musculus(house mouse)
Definition Mus musculus signal recognition particle 54A (Srp54a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_030246710.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTTCAAAG GAGCCTTTGA CCAGCTAAAA CAGAATGCCA CCAAAGCAAG AATTCCGTTC 
TATGGCAGCT ATACTGAAAT GGATCCTGTC ATCATTGCTT CTGAAGGAGT GGAGAAATTC 
AAAAATGAAA ATTTTGAAAT TATTATTGTT GATACAAGTG GTCGTCACAA ACAAGAAGAC 
TCTTTATTTG AAGAAATGCT TCAAGTTTCT AATGCTATAC AACCTGATAA CATTGTTTAT 
GTGATGGATG CATCCATCGG ACAGGCTTGT GAGGCCCAGG CGAAGGCCTT TAAAGACAAA 
GTAGATGTAG CTTCAGTAAT AGTGACAAAA CTCGACGGTC ATGCGAAAGG AGGCGGTGCT 
CTTAGTGCAG TTGCTGCCAC AAAAAGTCCA ATTATTTTCA TTGGTACAGG GGAACATATA 
GATGATTTTG AACCTTTCAA AACACAACCT TTCATCAGCA AACTCCTTGG AATGGGTGAT 
ATTGAAGGAC TGATTGATAA AGTCAATGAA TTGAAGTTGG ATGATAATGA GGCACTTATA 
GAGAAGTTGA AGCACGGTCA GTTTACATTG CGAGACATGT ATGAACAGTT TCAGAATATT 
ATGAAAATGG GCCCATTCAG TCAGATATTG GGGATGATTC CTGGCTTTGG CACAGATTTT 
ATGAGCAAAG GGAATGAGCA GGAGTCAATG GCAAGGCTGA AGAAACTGAT GACAATCATG 
GACAGTATGA ACGATCAAGA ACTGGACAGT ACAGATGGTG CCAAGGTTTT CAGTAAGCAA 
CCAGGGAGAA TCCAAAGAGT TGCCCGGGGA TCAGGTGTGT CAACAAGAGA TGTTCAAGAA 
CTTCTGACCC AGTATACCAA GTTTGCACAG ATGGTCAAAA AGATGGGAGG TATCAAAGGA 
CTTTTCAAAG GCGGTGATAT GTCTAAGAAT GTGAGTCAGT CACAGATGGC AAAATTAAAC 
CAACAAATGG CCAAAATGAT GGACCCACGA GTTCTTCATC ACATGGGTGG CATGGCCGGC 
CTTCAGTCAA TGATGCGGCA GTTTCAGCAG GGTGCTGCTG GCAACATGAA AGGCATGATG 
GGATTCAATA ACATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Structure of the signal recognition particle interacting with the elongation-arrested ribosome.
Nature427(6977)808-14(2004 Feb)
Halic M,Becker T,Pool MR,Spahn CM,Grassucci RA,Frank J,Beckmann R


book

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE


book

The methionine-rich domain of the 54 kd protein subunit of the signal recognition particle contains an RNA binding site and can be crosslinked to a signal sequence.
The EMBO journal9(13)4511-7(1990 Dec)
Zopf D,Bernstein HD,Johnson AE,Walter P


book

Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle.
Nature340(6233)482-6(1989 Aug)
Bernstein HD,Poritz MA,Strub K,Hoben PJ,Brenner S,Walter P