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IL27 cDNA ORF clone, Mus musculus(House mouse)

Gene Symbol IL27
Entrez Gene ID 246779
Full Name interleukin 27
Synonyms IL-27,IL-27p28,Il30,p28
General protein information
Preferred Names

interleukin 27

Names

interleukin-27 subunit alpha
IL-27 p28 subunit
IL-27 subunit alpha
IL-27-A
IL27-A
interleukin 30

Gene Type protein-coding
Organism Mus musculus(House mouse)
Genome

7

7|7 F3


mRNA and Protein(s)

mRNA Protein Name
XM_006507857.1 XP_006507920.1 interleukin-27 subunit alpha isoform X1
NM_145636.1 NP_663611.1 interleukin-27 subunit alpha precursor



Rattus norvegicus (Norway rat) Il27 XP_344963.4
Canis lupus familiaris (dog) IL27 XP_849829.2
Mus musculus (house mouse) Il27 NP_663611.1
Homo sapiens (human) IL27 NP_663634.2
Pan troglodytes (chimpanzee) IL27 XP_001138224.1
Bos taurus (cattle) IL27 NP_001158125.1


Related articles in PubMed

IL-27 Facilitates Skin Wound Healing through Induction of Epidermal Proliferation and Host Defense.
Yang B, Suwanpradid J, Sanchez-Lagunes R, Choi HW, Hoang P, Wang D, Abraham SN, MacLeod AS
The Journal of investigative dermatology137(5)1166-1175(2017 May)

Blockade of interleukin-27 signaling reduces GVHD in mice by augmenting Treg reconstitution and stabilizing Foxp3 expression.
Belle L, Agle K, Zhou V, Yin-Yuan C, Komorowski R, Eastwood D, Logan B, Sun J, Ghilardi N, Cua D, Williams CB, Gaignage M, Marillier R, van Snick J, Drobyski WR
Blood128(16)2068-2082(2016 Oct)

Interleukin-27-Producing CD4(+) T Cells Regulate Protective Immunity during Malaria Parasite Infection.
Kimura D, Miyakoda M, Kimura K, Honma K, Hara H, Yoshida H, Yui K
Immunity44(3)672-682(2016 Mar)

Promotion of Expansion and Differentiation of Hematopoietic Stem Cells by Interleukin-27 into Myeloid Progenitors to Control Infection in Emergency Myelopoiesis.
Furusawa J, Mizoguchi I, Chiba Y, Hisada M, Kobayashi F, Yoshida H, Nakae S, Tsuchida A, Matsumoto T, Ema H, Mizuguchi J, Yoshimoto T
PLoS pathogens12(3)e1005507(2016 Mar)

Transcription factor Fli-1 positively regulates lipopolysaccharide-induced interleukin-27 production in macrophages.
Gao P, Yuan M, Ma X, Jiang W, Zhu L, Wen M, Xu J, Liu Q, An H
Molecular immunology71184-91(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our data suggest a previously unrecognized role for IL-27 in regulating epithelial cell proliferation and antiviral host defense during the normal wound healing response.
Title: IL-27 Facilitates Skin Wound Healing through Induction of Epidermal Proliferation and Host Defense.

findings show that PGE2 inhibits IL-27 production in macrophages and in splenic conventional dendritic cells; and identify a novel pathway consisting of signaling through EP2/EP4-->induction of cAMP-->downregulation of IFN regulatory factor 1 expression and binding to the p28 IFN-stimulated response element site
Title: Prostaglandin E2 Inhibition of IL-27 Production in Murine Dendritic Cells: A Novel Mechanism That Involves IRF1.

These data indicate that endogenous IL-27 limits pathology during parainfluenza virus infection
Title: IL-27 Limits Type 2 Immunopathology Following Parainfluenza Virus Infection.

These data provide evidence of a central role of IL-27 for the control of Th2-mediated allergic diseases.
Title: IL-27 Is Essential for Suppression of Experimental Allergic Asthma by the TLR7/8 Agonist R848 (Resiquimod).

these data provide the first evidence that IL-27 signals directly to B cells promote GCs and support the role of IL-27 in lupus.
Title: IL-27 Directly Enhances Germinal Center B Cell Activity and Potentiates Lupus in Sanroque Mice.

The following IL27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OMu19354 NM_145636.1
Latest version!
Mus musculus interleukin 27 (Il27), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OMu39656 XM_006507857.1
Latest version!
Mus musculus interleukin 27 (Il27), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu19354
Clone ID Related Accession (Same CDS sequence) NM_145636.1
Accession Version NM_145636.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 705bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Mus musculus(House mouse)
Product interleukin-27 subunit alpha precursor
Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY099297.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY099297.1, BC119402.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGGCCAGG TGACAGGAGA CCTTGGCTGG CGGCTCAGCC TGTTGCTGCT ACCCTTGCTT 
CTGGTACAAG CTGGTTCCTG GGGGTTCCCA ACAGACCCCC TGAGCCTTCA AGAGCTGCGC
AGGGAATTCA CAGTCAGCCT GTACCTTGCC AGGAAGCTGC TCTCTGAGGT TCAGGGCTAT
GTCCACAGCT TTGCTGAATC TCGATTGCCA GGAGTGAACC TGGACCTCCT GCCCCTGGGA
TACCATCTTC CCAATGTTTC CCTGACTTTC CAGGCATGGC ATCACCTCTC TGACTCTGAG
AGACTCTGCT TCCTCGCTAC CACACTTCGG CCCTTCCCTG CCATGCTGGG AGGGCTGGGG
ACCCAGGGGA CCTGGACCAG CTCAGAGAGG GAGCAGCTGT GGGCCATGAG GCTGGATCTC
CGGGACCTGC ACAGGCACCT CCGCTTTCAG GTGCTGGCTG CAGGATTCAA ATGTTCAAAG
GAGGAGGAGG ACAAGGAGGA AGAGGAAGAG GAGGAAGAAG AAGAAAAGAA GCTGCCCCTA
GGGGCTCTGG GTGGCCCCAA TCAGGTGTCA TCCCAAGTGT CCTGGCCCCA GCTGCTCTAT
ACCTACCAGC TCCTTCACTC CCTGGAGCTT GTCCTGTCTC GGGCTGTTCG GGACCTGCTG
CTGCTGTCCC TGCCCAGGCG CCCAGGCTCA GCCTGGGATT CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_663611.1
CDS1..705
Exon (1)1..31
Exon (2)32..192
Exon (3)193..291
Exon (4)292..450
Exon (5)451..705
Translation

Target ORF information:

RefSeq Version NM_145636.1
Organism Mus musculus(House mouse)
Definition Mus musculus interleukin 27 (Il27), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_145636.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGGCCAGG TGACAGGAGA CCTTGGCTGG CGGCTCAGCC TGTTGCTGCT ACCCTTGCTT 
CTGGTACAAG CTGGTTCCTG GGGGTTCCCA ACAGACCCCC TGAGCCTTCA AGAGCTGCGC
AGGGAATTCA CAGTCAGCCT GTACCTTGCC AGGAAGCTGC TCTCTGAGGT TCAGGGCTAT
GTCCACAGCT TTGCTGAATC TCGATTGCCA GGAGTGAACC TGGACCTCCT GCCCCTGGGA
TACCATCTTC CCAATGTTTC CCTGACTTTC CAGGCATGGC ATCACCTCTC TGACTCTGAG
AGACTCTGCT TCCTCGCTAC CACACTTCGG CCCTTCCCTG CCATGCTGGG AGGGCTGGGG
ACCCAGGGGA CCTGGACCAG CTCAGAGAGG GAGCAGCTGT GGGCCATGAG GCTGGATCTC
CGGGACCTGC ACAGGCACCT CCGCTTTCAG GTGCTGGCTG CAGGATTCAA ATGTTCAAAG
GAGGAGGAGG ACAAGGAGGA AGAGGAAGAG GAGGAAGAAG AAGAAAAGAA GCTGCCCCTA
GGGGCTCTGG GTGGCCCCAA TCAGGTGTCA TCCCAAGTGT CCTGGCCCCA GCTGCTCTAT
ACCTACCAGC TCCTTCACTC CCTGGAGCTT GTCCTGTCTC GGGCTGTTCG GGACCTGCTG
CTGCTGTCCC TGCCCAGGCG CCCAGGCTCA GCCTGGGATT CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu39656
Clone ID Related Accession (Same CDS sequence) XM_006507857.1
Accession Version XM_006507857.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 789bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product interleukin-27 subunit alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGCCAGG TGACAGGAGA CCTTGGCTGG CGGCTCAGCC TGTTGCTGCT ACCCTTGCTT 
CTGGTACAAG CTGGTTCCTG GGGGTTCCCA ACAGACCCCC TGAGCCTTCA AGAGCTGCGC
AGGGAATTCA CAGTCAGCCT GTACCTTGCC AGGAAGCTGC TCTCTGAGGT TCAGGGCTAT
GTCCACAGCT TTGTGAGTCT CCTTAGCGCA ATTGGGTCTG GAGGTGAGGA CACCCCCAAG
TCCCACTGGC CCTCACTAAC AGAATTTAAC CCTCAGGCTG AATCTCGATT GCCAGGAGTG
AACCTGGACC TCCTGCCCCT GGGATACCAT CTTCCCAATG TTTCCCTGAC TTTCCAGGCA
TGGCATCACC TCTCTGACTC TGAGAGACTC TGCTTCCTCG CTACCACACT TCGGCCCTTC
CCTGCCATGC TGGGAGGGCT GGGGACCCAG GGGACCTGGA CCAGCTCAGA GAGGGAGCAG
CTGTGGGCCA TGAGGCTGGA TCTCCGGGAC CTGCACAGGC ACCTCCGCTT TCAGGTGCTG
GCTGCAGGAT TCAAATGTTC AAAGGAGGAG GAGGACAAGG AGGAAGAGGA AGAGGAGGAA
GAAGAAGAAA AGAAGCTGCC CCTAGGGGCT CTGGGTGGCC CCAATCAGGT GTCATCCCAA
GTGTCCTGGC CCCAGCTGCT CTATACCTAC CAGCTCCTTC ACTCCCTGGA GCTTGTCCTG
TCTCGGGCTG TTCGGGACCT GCTGCTGCTG TCCCTGCCCA GGCGCCCAGG CTCAGCCTGG
GATTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006507920.1
CDS112..900
Translation

Target ORF information:

RefSeq Version XM_006507857.1
Organism Mus musculus(House mouse)
Definition Mus musculus interleukin 27 (Il27), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006507857.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGCCAGG TGACAGGAGA CCTTGGCTGG CGGCTCAGCC TGTTGCTGCT ACCCTTGCTT 
CTGGTACAAG CTGGTTCCTG GGGGTTCCCA ACAGACCCCC TGAGCCTTCA AGAGCTGCGC
AGGGAATTCA CAGTCAGCCT GTACCTTGCC AGGAAGCTGC TCTCTGAGGT TCAGGGCTAT
GTCCACAGCT TTGTGAGTCT CCTTAGCGCA ATTGGGTCTG GAGGTGAGGA CACCCCCAAG
TCCCACTGGC CCTCACTAAC AGAATTTAAC CCTCAGGCTG AATCTCGATT GCCAGGAGTG
AACCTGGACC TCCTGCCCCT GGGATACCAT CTTCCCAATG TTTCCCTGAC TTTCCAGGCA
TGGCATCACC TCTCTGACTC TGAGAGACTC TGCTTCCTCG CTACCACACT TCGGCCCTTC
CCTGCCATGC TGGGAGGGCT GGGGACCCAG GGGACCTGGA CCAGCTCAGA GAGGGAGCAG
CTGTGGGCCA TGAGGCTGGA TCTCCGGGAC CTGCACAGGC ACCTCCGCTT TCAGGTGCTG
GCTGCAGGAT TCAAATGTTC AAAGGAGGAG GAGGACAAGG AGGAAGAGGA AGAGGAGGAA
GAAGAAGAAA AGAAGCTGCC CCTAGGGGCT CTGGGTGGCC CCAATCAGGT GTCATCCCAA
GTGTCCTGGC CCCAGCTGCT CTATACCTAC CAGCTCCTTC ACTCCCTGGA GCTTGTCCTG
TCTCGGGCTG TTCGGGACCT GCTGCTGCTG TCCCTGCCCA GGCGCCCAGG CTCAGCCTGG
GATTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

IL-27 Facilitates Skin Wound Healing through Induction of Epidermal Proliferation and Host Defense.
The Journal of investigative dermatology137(5)1166-1175(2017 May)
Yang B,Suwanpradid J,Sanchez-Lagunes R,Choi HW,Hoang P,Wang D,Abraham SN,MacLeod AS


book

Blockade of interleukin-27 signaling reduces GVHD in mice by augmenting Treg reconstitution and stabilizing Foxp3 expression.
Blood128(16)2068-2082(2016 Oct)
Belle L,Agle K,Zhou V,Yin-Yuan C,Komorowski R,Eastwood D,Logan B,Sun J,Ghilardi N,Cua D,Williams CB,Gaignage M,Marillier R,van Snick J,Drobyski WR


book

Interleukin-27-Producing CD4(+) T Cells Regulate Protective Immunity during Malaria Parasite Infection.
Immunity44(3)672-682(2016 Mar)
Kimura D,Miyakoda M,Kimura K,Honma K,Hara H,Yoshida H,Yui K


book

Promotion of Expansion and Differentiation of Hematopoietic Stem Cells by Interleukin-27 into Myeloid Progenitors to Control Infection in Emergency Myelopoiesis.
PLoS pathogens12(3)e1005507(2016 Mar)
Furusawa J,Mizoguchi I,Chiba Y,Hisada M,Kobayashi F,Yoshida H,Nakae S,Tsuchida A,Matsumoto T,Ema H,Mizuguchi J,Yoshimoto T


book

Transcription factor Fli-1 positively regulates lipopolysaccharide-induced interleukin-27 production in macrophages.
Molecular immunology71184-91(2016 Mar)
Gao P,Yuan M,Ma X,Jiang W,Zhu L,Wen M,Xu J,Liu Q,An H


 
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