Mios cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Mios
Entrez Gene ID	252875
Full Name	meiosis regulator for oocyte development
Synonyms	C81488
General protein information	Preferred Names meiosis regulator for oocyte development Names GATOR complex protein MIOS WD repeat-containing protein mio missing oocyte, meiosis regulator, homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006505086.3	XP_006505149.1	WD repeat-containing protein mio isoform X1
NM_145374.2	NP_663349.2	GATOR complex protein MIOS
XM_006505086.4	XP_006505149.1	GATOR complex protein MIOS isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MIOS	XP_001144783.1
Drosophila melanogaster (fruit fly)	mio	NP_608656.1
Xenopus tropicalis (tropical clawed frog)	mios	XP_004915476.1
	MIOS	XP_001087456.1
Canis lupus familiaris (dog)	MIOS	XP_005628601.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007984	XP_317482.4
Homo sapiens (human)	MIOS	NP_061878.3
Bos taurus (cattle)	MIOS	NP_001179247.1
Gallus gallus (chicken)	MIOS	XP_418678.2
Rattus norvegicus (Norway rat)	Mios	NP_001167025.1
Mus musculus (house mouse)	Mios	NP_663349.2
Danio rerio (zebrafish)	mios	NP_958490.1

Gene Ontology
Bibliography

Related articles in PubMed

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Lumayag S, Haldin CE, Corbett NJ, Wahlin KJ, Cowan C, Turturro S, Larsen PE, Kovacs B, Witmer PD, Valle D, Zack DJ, Nicholson DA, Xu S
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
Nishimura M, Yokoi N, Miki T, Horikawa Y, Yoshioka H, Takeda J, Ohara O, Seino S
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mios gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mios cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21561	NM_145374.2 Latest version!	Mus musculus meiosis regulator for oocyte development (Mios), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu21561	XM_006505086.4 Latest version!	Mus musculus meiosis regulator for oocyte development (Mios), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu21561	XM_006505086.3	Mus musculus missing oocyte, meiosis regulator, homolog (Drosophila) (Mios), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mios ( NM_145374.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663349.2 Mus musculus meiosis regulator for oocyte development (Mios), mRNA.

Price & Availability↑

CloneID	OMu21561
Clone ID Related Accession (Same CDS sequence)	XM_006505086.3 , NM_145374.2 , XM_006505086.4
Accession Version	NM_145374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	GATOR complex protein MIOS
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY064617.1, BU510538.1, CX565910.1, CD807728.1, BQ876300.1, AK079844.1, AK034623.1 and BP765255.1. On Nov 15, 2008 this sequence version replaced NM_145374.1. ##Evidence-Data-START## Transcript exon combination :: BC020002.1, SRR1660819.145003.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGGTA CCAAGCCCGA TATTTTGTGG GCACCACACC AAGTTGATAG ATTTGTTGTA 
TGTGACTCAG AATTGAGCCT TTATCATGTG GAATCGGCTG TGAATTCAGA ACTCAAAGCT 
GGATCGTTAC GTTTATCTGA AGACTCTGCA GCGACATTGC TGTCAATAAA CTCAGATACA 
CCCTATATGA AATGTGTTGC GTGGTATCTC AATTATGATC CTGAATGTCT CCTAGCAGTT 
GGACAAGCAA ATGGTCGAGT TGTACTTACA AGTCTTGGTC AAGATCATAA CTCTAAGTTC 
AAAGATTTGA TAGGAAAAGA ATTTGTTCCA AAGCATGCTC GCCAATGCAA TACCCTTGCA 
TGGAATCCAC TGGATAGTAA CTGGCTTGCT GCTGGTCTCG ACAAACACAG AGCTGACTTT 
TCAGTTCTAA TATGGGATAT CTGCAGCAAA TATACTCCTG ATATTGTTCC CATGGAAAAA 
GTGAGACTTT CAGCAGGTGA AGCTGAAACA ACATTACTAG TGACAAAACC GCTGTATGAG 
TTAGGGCAGA ATGACGCTTC TCTATCTCTG TGTTGGCTTC CACGAGACCA GAAACTTCTT 
CTTGCTGGCA TGCATCGTAA TCTAGCAATA TTTGATCTCC GGAACACAAG CCAGAAGATG 
TTTGTAAATA CAAAAGCTGT TCAAGGAGTG ACAGTAGACC CTTACTTCCA CGATCGTGTT 
GCTTCCTTCT ATGAAGGTCA GGTTGCAATA TGGGATCTTA GAAAATTTGA GAAGCCAGTT 
TTGACTTTGA CTGAACAACC AAAGCCCTTG ACAAAGGTCG CATGGTGTCC AACCAGGACA 
GGCCTGCTTG CTACTTTAAC AAGGGATAGT AATATTATTA GGCTGTATGA TATGCAGCAT 
ACACCCACTC CCATCGGGGA TGAAACCGAA CCCACAATAA TTGAAAGAAG TGTGCAGCCT 
TGTGACAATT ACATTGCTTC CTTTGCATGG CATCCAACGA GTCAAAATCG AATGATAGTT 
GTGACTCCCA ACCGAACAAT GTCTGACTTC ACTGTATTTG AAAGGATATC TCTTGCCTGG 
AGCCCAATTA CATCTTTAAT GTGGGCTTGT GGTCGCCATT TGTATGAGTG TGCAGAAGAA 
GAAAGTGATA ACTCCTTAGA AAAAGATATA GCAACAAAAA TGCGCCTTCG GGCTTTATCA 
AGGTATGGAC TTGATACAGA ACAGGTTTGG AGAAACCACA TTTTAGCTGG CAATGAAGAC 
CCACAGCTCA AGTCACTCTG GTATACTCTG CACTTTATGA AGCAATATAC AGAAGATAAT 
GATCAAAAAT CTCCAGGCAA CAAAGGATCA TTGGTTTATG CAGGAATTAA ATCAATAGTA 
AAATCATCTT TGGGAATGGT AGAAAGCAGC AGACATAATT GGAGTGGCTT GGATAAACAA 
ACTGATATTC AGAACTTAAA TGAAGAAAGG ATTTTAGCAT TACAGCTTTG TGGGTGGATA 
AAGAAAGGAA CGGATGTAGA TGTAGGGCCA TTTTTGAACT CCCTTGTGCA AGAAGGAGAA 
TGGGAAAGAG CTGCTGCTGT GGCTTTGTTC AACTTGGATA TTCGGCGAGC AATCCAAATC 
CTGAATGAAG GGGCGTCTTC AGAAAAAGGA GATCTGAATC TCAATGTGGT GGCAATGGCT 
TTATCGGGGT ATACGGACGA GAAGAACTCG CTGTGGCGGG AGATGTGCAG CACCCTGCGC 
CTGCAGCTCA ACAACCCCTA TCTGTGTGTC ATGTTTGCGT TCCTGACGAG TGAAGCGGGG 
GCATATGATG GAGTGTTGTA TGAAAACAAA GTTGCTGTAC GTGACAGAGT GGCATTCGCT 
TGTAAATTCC TTGGTGATGC TCAGTTAAAT AAATACATTG AAAAGCTGAC CAATGAAATG 
AAAGAGGCTG GAAATTTGGA GGGGATCTTG CTTACAGGCC TCACTAAAGA TGGAGTGGAC 
CTGATGGAGA GCTATGTGGA TAGAACTGGA GATGTTCAAA CAGCAAGTTA CTGCATGTTA 
CAGGGTTCAC CTTTAGATGT TCTGAAAGAT GAAAGGGTTC AATATTGGAT TGAGAATTAC 
AGAAATTTAT TAGATGCCTG GAGGTTTTGG CACAAAAGGG CAGAATTTGA TATTCATAGG 
AGTAAGCTGG ATCCCAGTTC CAAGCCTTTA GCACAGGTGT TTGTGAGTTG TAATTTTTGT 
GGCAAATCAA TCTCCTACAG CTGTTCCTCT GTGCCTCATC AGGGCAGAGG GTTTAGTCAG 
TATGGTGTGA GTGGCTCACC AACAAAATCC AAAGTCACAA GCTGCCCTGG CTGTCGGAAA 
CCCCTTCCTC GATGTGCACT TTGCCTCATT AATATGGGAA CACCTGTTTC TAGCTGTCCT 
GGTGGATCCA AATCAGATGA AAAGGTGGAC TTGAGCAAGG ACAAAAAGTT AGCTCAGTTT 
AACAACTGGT TTACCTGGTG TCACAACTGT CGGCATGGTG GGCATGCCGG ACATATGCTG 
AGTTGGTTCA GGGACCATGC AGAGTGTCCT GTATCTGCAT GCACGTGTAA ATGTATGCAG 
TTGGATACAA CAGGAAATCT GGTGCCAGCA GAGACTGTCC AGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663349.2
CDS	285..2912
Translation	MSGTKPDILWAPHQVDRFVVCDSELSLYHVESAVNSELKAGSLRLSEDSAATLLSINSDTPYMKCVAWYLNYDPECLLAVGQANGRVVLTSLGQDHNSKFKDLIGKEFVPKHARQCNTLAWNPLDSNWLAAGLDKHRADFSVLIWDICSKYTPDIVPMEKVRLSAGEAETTLLVTKPLYELGQNDASLSLCWLPRDQKLLLAGMHRNLAIFDLRNTSQKMFVNTKAVQGVTVDPYFHDRVASFYEGQVAIWDLRKFEKPVLTLTEQPKPLTKVAWCPTRTGLLATLTRDSNIIRLYDMQHTPTPIGDETEPTIIERSVQPCDNYIASFAWHPTSQNRMIVVTPNRTMSDFTVFERISLAWSPITSLMWACGRHLYECAEEESDNSLEKDIATKMRLRALSRYGLDTEQVWRNHILAGNEDPQLKSLWYTLHFMKQYTEDNDQKSPGNKGSLVYAGIKSIVKSSLGMVESSRHNWSGLDKQTDIQNLNEERILALQLCGWIKKGTDVDVGPFLNSLVQEGEWERAAAVALFNLDIRRAIQILNEGASSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNNPYLCVMFAFLTSEAGAYDGVLYENKVAVRDRVAFACKFLGDAQLNKYIEKLTNEMKEAGNLEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLDAWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSSVPHQGRGFSQYGVSGSPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGSKSDEKVDLSKDKKLAQFNNWFTWCHNCRHGGHAGHMLSWFRDHAECPVSACTCKCMQLDTTGNLVPAETVQP

Target ORF information:

RefSeq Version	NM_145374.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus meiosis regulator for oocyte development (Mios), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145374.2

ORF Insert Sequence:

ATGAGCGGTA CCAAGCCCGA TATTTTGTGG GCACCACACC AAGTTGATAG ATTTGTTGTA 
TGTGACTCAG AATTGAGCCT TTATCATGTG GAATCGGCTG TGAATTCAGA ACTCAAAGCT 
GGATCGTTAC GTTTATCTGA AGACTCTGCA GCGACATTGC TGTCAATAAA CTCAGATACA 
CCCTATATGA AATGTGTTGC GTGGTATCTC AATTATGATC CTGAATGTCT CCTAGCAGTT 
GGACAAGCAA ATGGTCGAGT TGTACTTACA AGTCTTGGTC AAGATCATAA CTCTAAGTTC 
AAAGATTTGA TAGGAAAAGA ATTTGTTCCA AAGCATGCTC GCCAATGCAA TACCCTTGCA 
TGGAATCCAC TGGATAGTAA CTGGCTTGCT GCTGGTCTCG ACAAACACAG AGCTGACTTT 
TCAGTTCTAA TATGGGATAT CTGCAGCAAA TATACTCCTG ATATTGTTCC CATGGAAAAA 
GTGAGACTTT CAGCAGGTGA AGCTGAAACA ACATTACTAG TGACAAAACC GCTGTATGAG 
TTAGGGCAGA ATGACGCTTC TCTATCTCTG TGTTGGCTTC CACGAGACCA GAAACTTCTT 
CTTGCTGGCA TGCATCGTAA TCTAGCAATA TTTGATCTCC GGAACACAAG CCAGAAGATG 
TTTGTAAATA CAAAAGCTGT TCAAGGAGTG ACAGTAGACC CTTACTTCCA CGATCGTGTT 
GCTTCCTTCT ATGAAGGTCA GGTTGCAATA TGGGATCTTA GAAAATTTGA GAAGCCAGTT 
TTGACTTTGA CTGAACAACC AAAGCCCTTG ACAAAGGTCG CATGGTGTCC AACCAGGACA 
GGCCTGCTTG CTACTTTAAC AAGGGATAGT AATATTATTA GGCTGTATGA TATGCAGCAT 
ACACCCACTC CCATCGGGGA TGAAACCGAA CCCACAATAA TTGAAAGAAG TGTGCAGCCT 
TGTGACAATT ACATTGCTTC CTTTGCATGG CATCCAACGA GTCAAAATCG AATGATAGTT 
GTGACTCCCA ACCGAACAAT GTCTGACTTC ACTGTATTTG AAAGGATATC TCTTGCCTGG 
AGCCCAATTA CATCTTTAAT GTGGGCTTGT GGTCGCCATT TGTATGAGTG TGCAGAAGAA 
GAAAGTGATA ACTCCTTAGA AAAAGATATA GCAACAAAAA TGCGCCTTCG GGCTTTATCA 
AGGTATGGAC TTGATACAGA ACAGGTTTGG AGAAACCACA TTTTAGCTGG CAATGAAGAC 
CCACAGCTCA AGTCACTCTG GTATACTCTG CACTTTATGA AGCAATATAC AGAAGATAAT 
GATCAAAAAT CTCCAGGCAA CAAAGGATCA TTGGTTTATG CAGGAATTAA ATCAATAGTA 
AAATCATCTT TGGGAATGGT AGAAAGCAGC AGACATAATT GGAGTGGCTT GGATAAACAA 
ACTGATATTC AGAACTTAAA TGAAGAAAGG ATTTTAGCAT TACAGCTTTG TGGGTGGATA 
AAGAAAGGAA CGGATGTAGA TGTAGGGCCA TTTTTGAACT CCCTTGTGCA AGAAGGAGAA 
TGGGAAAGAG CTGCTGCTGT GGCTTTGTTC AACTTGGATA TTCGGCGAGC AATCCAAATC 
CTGAATGAAG GGGCGTCTTC AGAAAAAGGA GATCTGAATC TCAATGTGGT GGCAATGGCT 
TTATCGGGGT ATACGGACGA GAAGAACTCG CTGTGGCGGG AGATGTGCAG CACCCTGCGC 
CTGCAGCTCA ACAACCCCTA TCTGTGTGTC ATGTTTGCGT TCCTGACGAG TGAAGCGGGG 
GCATATGATG GAGTGTTGTA TGAAAACAAA GTTGCTGTAC GTGACAGAGT GGCATTCGCT 
TGTAAATTCC TTGGTGATGC TCAGTTAAAT AAATACATTG AAAAGCTGAC CAATGAAATG 
AAAGAGGCTG GAAATTTGGA GGGGATCTTG CTTACAGGCC TCACTAAAGA TGGAGTGGAC 
CTGATGGAGA GCTATGTGGA TAGAACTGGA GATGTTCAAA CAGCAAGTTA CTGCATGTTA 
CAGGGTTCAC CTTTAGATGT TCTGAAAGAT GAAAGGGTTC AATATTGGAT TGAGAATTAC 
AGAAATTTAT TAGATGCCTG GAGGTTTTGG CACAAAAGGG CAGAATTTGA TATTCATAGG 
AGTAAGCTGG ATCCCAGTTC CAAGCCTTTA GCACAGGTGT TTGTGAGTTG TAATTTTTGT 
GGCAAATCAA TCTCCTACAG CTGTTCCTCT GTGCCTCATC AGGGCAGAGG GTTTAGTCAG 
TATGGTGTGA GTGGCTCACC AACAAAATCC AAAGTCACAA GCTGCCCTGG CTGTCGGAAA 
CCCCTTCCTC GATGTGCACT TTGCCTCATT AATATGGGAA CACCTGTTTC TAGCTGTCCT 
GGTGGATCCA AATCAGATGA AAAGGTGGAC TTGAGCAAGG ACAAAAAGTT AGCTCAGTTT 
AACAACTGGT TTACCTGGTG TCACAACTGT CGGCATGGTG GGCATGCCGG ACATATGCTG 
AGTTGGTTCA GGGACCATGC AGAGTGTCCT GTATCTGCAT GCACGTGTAA ATGTATGCAG 
TTGGATACAA CAGGAAATCT GGTGCCAGCA GAGACTGTCC AGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mios ( XM_006505086.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505149.1 Mus musculus missing oocyte, meiosis regulator, homolog (Drosophila) (Mios), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu21561
Clone ID Related Accession (Same CDS sequence)	XM_006505086.3 , NM_145374.2 , XM_006505086.4
Accession Version	XM_006505086.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	WD repeat-containing protein mio isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006505086.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGTA CCAAGCCCGA TATTTTGTGG GCACCACACC AAGTTGATAG ATTTGTTGTA 
TGTGACTCAG AATTGAGCCT TTATCATGTG GAATCGGCTG TGAATTCAGA ACTCAAAGCT 
GGATCGTTAC GTTTATCTGA AGACTCTGCA GCGACATTGC TGTCAATAAA CTCAGATACA 
CCCTATATGA AATGTGTTGC GTGGTATCTC AATTATGATC CTGAATGTCT CCTAGCAGTT 
GGACAAGCAA ATGGTCGAGT TGTACTTACA AGTCTTGGTC AAGATCATAA CTCTAAGTTC 
AAAGATTTGA TAGGAAAAGA ATTTGTTCCA AAGCATGCTC GCCAATGCAA TACCCTTGCA 
TGGAATCCAC TGGATAGTAA CTGGCTTGCT GCTGGTCTCG ACAAACACAG AGCTGACTTT 
TCAGTTCTAA TATGGGATAT CTGCAGCAAA TATACTCCTG ATATTGTTCC CATGGAAAAA 
GTGAGACTTT CAGCAGGTGA AGCTGAAACA ACATTACTAG TGACAAAACC GCTGTATGAG 
TTAGGGCAGA ATGACGCTTC TCTATCTCTG TGTTGGCTTC CACGAGACCA GAAACTTCTT 
CTTGCTGGCA TGCATCGTAA TCTAGCAATA TTTGATCTCC GGAACACAAG CCAGAAGATG 
TTTGTAAATA CAAAAGCTGT TCAAGGAGTG ACAGTAGACC CTTACTTCCA CGATCGTGTT 
GCTTCCTTCT ATGAAGGTCA GGTTGCAATA TGGGATCTTA GAAAATTTGA GAAGCCAGTT 
TTGACTTTGA CTGAACAACC AAAGCCCTTG ACAAAGGTCG CATGGTGTCC AACCAGGACA 
GGCCTGCTTG CTACTTTAAC AAGGGATAGT AATATTATTA GGCTGTATGA TATGCAGCAT 
ACACCCACTC CCATCGGGGA TGAAACCGAA CCCACAATAA TTGAAAGAAG TGTGCAGCCT 
TGTGACAATT ACATTGCTTC CTTTGCATGG CATCCAACGA GTCAAAATCG AATGATAGTT 
GTGACTCCCA ACCGAACAAT GTCTGACTTC ACTGTATTTG AAAGGATATC TCTTGCCTGG 
AGCCCAATTA CATCTTTAAT GTGGGCTTGT GGTCGCCATT TGTATGAGTG TGCAGAAGAA 
GAAAGTGATA ACTCCTTAGA AAAAGATATA GCAACAAAAA TGCGCCTTCG GGCTTTATCA 
AGGTATGGAC TTGATACAGA ACAGGTTTGG AGAAACCACA TTTTAGCTGG CAATGAAGAC 
CCACAGCTCA AGTCACTCTG GTATACTCTG CACTTTATGA AGCAATATAC AGAAGATAAT 
GATCAAAAAT CTCCAGGCAA CAAAGGATCA TTGGTTTATG CAGGAATTAA ATCAATAGTA 
AAATCATCTT TGGGAATGGT AGAAAGCAGC AGACATAATT GGAGTGGCTT GGATAAACAA 
ACTGATATTC AGAACTTAAA TGAAGAAAGG ATTTTAGCAT TACAGCTTTG TGGGTGGATA 
AAGAAAGGAA CGGATGTAGA TGTAGGGCCA TTTTTGAACT CCCTTGTGCA AGAAGGAGAA 
TGGGAAAGAG CTGCTGCTGT GGCTTTGTTC AACTTGGATA TTCGGCGAGC AATCCAAATC 
CTGAATGAAG GGGCGTCTTC AGAAAAAGGA GATCTGAATC TCAATGTGGT GGCAATGGCT 
TTATCGGGGT ATACGGACGA GAAGAACTCG CTGTGGCGGG AGATGTGCAG CACCCTGCGC 
CTGCAGCTCA ACAACCCCTA TCTGTGTGTC ATGTTTGCGT TCCTGACGAG TGAAGCGGGG 
GCATATGATG GAGTGTTGTA TGAAAACAAA GTTGCTGTAC GTGACAGAGT GGCATTCGCT 
TGTAAATTCC TTGGTGATGC TCAGTTAAAT AAATACATTG AAAAGCTGAC CAATGAAATG 
AAAGAGGCTG GAAATTTGGA GGGGATCTTG CTTACAGGCC TCACTAAAGA TGGAGTGGAC 
CTGATGGAGA GCTATGTGGA TAGAACTGGA GATGTTCAAA CAGCAAGTTA CTGCATGTTA 
CAGGGTTCAC CTTTAGATGT TCTGAAAGAT GAAAGGGTTC AATATTGGAT TGAGAATTAC 
AGAAATTTAT TAGATGCCTG GAGGTTTTGG CACAAAAGGG CAGAATTTGA TATTCATAGG 
AGTAAGCTGG ATCCCAGTTC CAAGCCTTTA GCACAGGTGT TTGTGAGTTG TAATTTTTGT 
GGCAAATCAA TCTCCTACAG CTGTTCCTCT GTGCCTCATC AGGGCAGAGG GTTTAGTCAG 
TATGGTGTGA GTGGCTCACC AACAAAATCC AAAGTCACAA GCTGCCCTGG CTGTCGGAAA 
CCCCTTCCTC GATGTGCACT TTGCCTCATT AATATGGGAA CACCTGTTTC TAGCTGTCCT 
GGTGGATCCA AATCAGATGA AAAGGTGGAC TTGAGCAAGG ACAAAAAGTT AGCTCAGTTT 
AACAACTGGT TTACCTGGTG TCACAACTGT CGGCATGGTG GGCATGCCGG ACATATGCTG 
AGTTGGTTCA GGGACCATGC AGAGTGTCCT GTATCTGCAT GCACGTGTAA ATGTATGCAG 
TTGGATACAA CAGGAAATCT GGTGCCAGCA GAGACTGTCC AGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505149.1
CDS	461..3088
Translation	MSGTKPDILWAPHQVDRFVVCDSELSLYHVESAVNSELKAGSLRLSEDSAATLLSINSDTPYMKCVAWYLNYDPECLLAVGQANGRVVLTSLGQDHNSKFKDLIGKEFVPKHARQCNTLAWNPLDSNWLAAGLDKHRADFSVLIWDICSKYTPDIVPMEKVRLSAGEAETTLLVTKPLYELGQNDASLSLCWLPRDQKLLLAGMHRNLAIFDLRNTSQKMFVNTKAVQGVTVDPYFHDRVASFYEGQVAIWDLRKFEKPVLTLTEQPKPLTKVAWCPTRTGLLATLTRDSNIIRLYDMQHTPTPIGDETEPTIIERSVQPCDNYIASFAWHPTSQNRMIVVTPNRTMSDFTVFERISLAWSPITSLMWACGRHLYECAEEESDNSLEKDIATKMRLRALSRYGLDTEQVWRNHILAGNEDPQLKSLWYTLHFMKQYTEDNDQKSPGNKGSLVYAGIKSIVKSSLGMVESSRHNWSGLDKQTDIQNLNEERILALQLCGWIKKGTDVDVGPFLNSLVQEGEWERAAAVALFNLDIRRAIQILNEGASSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNNPYLCVMFAFLTSEAGAYDGVLYENKVAVRDRVAFACKFLGDAQLNKYIEKLTNEMKEAGNLEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLDAWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSSVPHQGRGFSQYGVSGSPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGSKSDEKVDLSKDKKLAQFNNWFTWCHNCRHGGHAGHMLSWFRDHAECPVSACTCKCMQLDTTGNLVPAETVQP

Target ORF information:

RefSeq Version	XM_006505086.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus missing oocyte, meiosis regulator, homolog (Drosophila) (Mios), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505086.3

ORF Insert Sequence:

ATGAGCGGTA CCAAGCCCGA TATTTTGTGG GCACCACACC AAGTTGATAG ATTTGTTGTA 
TGTGACTCAG AATTGAGCCT TTATCATGTG GAATCGGCTG TGAATTCAGA ACTCAAAGCT 
GGATCGTTAC GTTTATCTGA AGACTCTGCA GCGACATTGC TGTCAATAAA CTCAGATACA 
CCCTATATGA AATGTGTTGC GTGGTATCTC AATTATGATC CTGAATGTCT CCTAGCAGTT 
GGACAAGCAA ATGGTCGAGT TGTACTTACA AGTCTTGGTC AAGATCATAA CTCTAAGTTC 
AAAGATTTGA TAGGAAAAGA ATTTGTTCCA AAGCATGCTC GCCAATGCAA TACCCTTGCA 
TGGAATCCAC TGGATAGTAA CTGGCTTGCT GCTGGTCTCG ACAAACACAG AGCTGACTTT 
TCAGTTCTAA TATGGGATAT CTGCAGCAAA TATACTCCTG ATATTGTTCC CATGGAAAAA 
GTGAGACTTT CAGCAGGTGA AGCTGAAACA ACATTACTAG TGACAAAACC GCTGTATGAG 
TTAGGGCAGA ATGACGCTTC TCTATCTCTG TGTTGGCTTC CACGAGACCA GAAACTTCTT 
CTTGCTGGCA TGCATCGTAA TCTAGCAATA TTTGATCTCC GGAACACAAG CCAGAAGATG 
TTTGTAAATA CAAAAGCTGT TCAAGGAGTG ACAGTAGACC CTTACTTCCA CGATCGTGTT 
GCTTCCTTCT ATGAAGGTCA GGTTGCAATA TGGGATCTTA GAAAATTTGA GAAGCCAGTT 
TTGACTTTGA CTGAACAACC AAAGCCCTTG ACAAAGGTCG CATGGTGTCC AACCAGGACA 
GGCCTGCTTG CTACTTTAAC AAGGGATAGT AATATTATTA GGCTGTATGA TATGCAGCAT 
ACACCCACTC CCATCGGGGA TGAAACCGAA CCCACAATAA TTGAAAGAAG TGTGCAGCCT 
TGTGACAATT ACATTGCTTC CTTTGCATGG CATCCAACGA GTCAAAATCG AATGATAGTT 
GTGACTCCCA ACCGAACAAT GTCTGACTTC ACTGTATTTG AAAGGATATC TCTTGCCTGG 
AGCCCAATTA CATCTTTAAT GTGGGCTTGT GGTCGCCATT TGTATGAGTG TGCAGAAGAA 
GAAAGTGATA ACTCCTTAGA AAAAGATATA GCAACAAAAA TGCGCCTTCG GGCTTTATCA 
AGGTATGGAC TTGATACAGA ACAGGTTTGG AGAAACCACA TTTTAGCTGG CAATGAAGAC 
CCACAGCTCA AGTCACTCTG GTATACTCTG CACTTTATGA AGCAATATAC AGAAGATAAT 
GATCAAAAAT CTCCAGGCAA CAAAGGATCA TTGGTTTATG CAGGAATTAA ATCAATAGTA 
AAATCATCTT TGGGAATGGT AGAAAGCAGC AGACATAATT GGAGTGGCTT GGATAAACAA 
ACTGATATTC AGAACTTAAA TGAAGAAAGG ATTTTAGCAT TACAGCTTTG TGGGTGGATA 
AAGAAAGGAA CGGATGTAGA TGTAGGGCCA TTTTTGAACT CCCTTGTGCA AGAAGGAGAA 
TGGGAAAGAG CTGCTGCTGT GGCTTTGTTC AACTTGGATA TTCGGCGAGC AATCCAAATC 
CTGAATGAAG GGGCGTCTTC AGAAAAAGGA GATCTGAATC TCAATGTGGT GGCAATGGCT 
TTATCGGGGT ATACGGACGA GAAGAACTCG CTGTGGCGGG AGATGTGCAG CACCCTGCGC 
CTGCAGCTCA ACAACCCCTA TCTGTGTGTC ATGTTTGCGT TCCTGACGAG TGAAGCGGGG 
GCATATGATG GAGTGTTGTA TGAAAACAAA GTTGCTGTAC GTGACAGAGT GGCATTCGCT 
TGTAAATTCC TTGGTGATGC TCAGTTAAAT AAATACATTG AAAAGCTGAC CAATGAAATG 
AAAGAGGCTG GAAATTTGGA GGGGATCTTG CTTACAGGCC TCACTAAAGA TGGAGTGGAC 
CTGATGGAGA GCTATGTGGA TAGAACTGGA GATGTTCAAA CAGCAAGTTA CTGCATGTTA 
CAGGGTTCAC CTTTAGATGT TCTGAAAGAT GAAAGGGTTC AATATTGGAT TGAGAATTAC 
AGAAATTTAT TAGATGCCTG GAGGTTTTGG CACAAAAGGG CAGAATTTGA TATTCATAGG 
AGTAAGCTGG ATCCCAGTTC CAAGCCTTTA GCACAGGTGT TTGTGAGTTG TAATTTTTGT 
GGCAAATCAA TCTCCTACAG CTGTTCCTCT GTGCCTCATC AGGGCAGAGG GTTTAGTCAG 
TATGGTGTGA GTGGCTCACC AACAAAATCC AAAGTCACAA GCTGCCCTGG CTGTCGGAAA 
CCCCTTCCTC GATGTGCACT TTGCCTCATT AATATGGGAA CACCTGTTTC TAGCTGTCCT 
GGTGGATCCA AATCAGATGA AAAGGTGGAC TTGAGCAAGG ACAAAAAGTT AGCTCAGTTT 
AACAACTGGT TTACCTGGTG TCACAACTGT CGGCATGGTG GGCATGCCGG ACATATGCTG 
AGTTGGTTCA GGGACCATGC AGAGTGTCCT GTATCTGCAT GCACGTGTAA ATGTATGCAG 
TTGGATACAA CAGGAAATCT GGTGCCAGCA GAGACTGTCC AGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mios ( XM_006505086.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505149.1 Mus musculus meiosis regulator for oocyte development (Mios), transcript variant X1, mRNA.

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CloneID	OMu21561
Clone ID Related Accession (Same CDS sequence)	XM_006505086.3 , NM_145374.2 , XM_006505086.4
Accession Version	XM_006505086.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GATOR complex protein MIOS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006505086.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGTA CCAAGCCCGA TATTTTGTGG GCACCACACC AAGTTGATAG ATTTGTTGTA 
TGTGACTCAG AATTGAGCCT TTATCATGTG GAATCGGCTG TGAATTCAGA ACTCAAAGCT 
GGATCGTTAC GTTTATCTGA AGACTCTGCA GCGACATTGC TGTCAATAAA CTCAGATACA 
CCCTATATGA AATGTGTTGC GTGGTATCTC AATTATGATC CTGAATGTCT CCTAGCAGTT 
GGACAAGCAA ATGGTCGAGT TGTACTTACA AGTCTTGGTC AAGATCATAA CTCTAAGTTC 
AAAGATTTGA TAGGAAAAGA ATTTGTTCCA AAGCATGCTC GCCAATGCAA TACCCTTGCA 
TGGAATCCAC TGGATAGTAA CTGGCTTGCT GCTGGTCTCG ACAAACACAG AGCTGACTTT 
TCAGTTCTAA TATGGGATAT CTGCAGCAAA TATACTCCTG ATATTGTTCC CATGGAAAAA 
GTGAGACTTT CAGCAGGTGA AGCTGAAACA ACATTACTAG TGACAAAACC GCTGTATGAG 
TTAGGGCAGA ATGACGCTTC TCTATCTCTG TGTTGGCTTC CACGAGACCA GAAACTTCTT 
CTTGCTGGCA TGCATCGTAA TCTAGCAATA TTTGATCTCC GGAACACAAG CCAGAAGATG 
TTTGTAAATA CAAAAGCTGT TCAAGGAGTG ACAGTAGACC CTTACTTCCA CGATCGTGTT 
GCTTCCTTCT ATGAAGGTCA GGTTGCAATA TGGGATCTTA GAAAATTTGA GAAGCCAGTT 
TTGACTTTGA CTGAACAACC AAAGCCCTTG ACAAAGGTCG CATGGTGTCC AACCAGGACA 
GGCCTGCTTG CTACTTTAAC AAGGGATAGT AATATTATTA GGCTGTATGA TATGCAGCAT 
ACACCCACTC CCATCGGGGA TGAAACCGAA CCCACAATAA TTGAAAGAAG TGTGCAGCCT 
TGTGACAATT ACATTGCTTC CTTTGCATGG CATCCAACGA GTCAAAATCG AATGATAGTT 
GTGACTCCCA ACCGAACAAT GTCTGACTTC ACTGTATTTG AAAGGATATC TCTTGCCTGG 
AGCCCAATTA CATCTTTAAT GTGGGCTTGT GGTCGCCATT TGTATGAGTG TGCAGAAGAA 
GAAAGTGATA ACTCCTTAGA AAAAGATATA GCAACAAAAA TGCGCCTTCG GGCTTTATCA 
AGGTATGGAC TTGATACAGA ACAGGTTTGG AGAAACCACA TTTTAGCTGG CAATGAAGAC 
CCACAGCTCA AGTCACTCTG GTATACTCTG CACTTTATGA AGCAATATAC AGAAGATAAT 
GATCAAAAAT CTCCAGGCAA CAAAGGATCA TTGGTTTATG CAGGAATTAA ATCAATAGTA 
AAATCATCTT TGGGAATGGT AGAAAGCAGC AGACATAATT GGAGTGGCTT GGATAAACAA 
ACTGATATTC AGAACTTAAA TGAAGAAAGG ATTTTAGCAT TACAGCTTTG TGGGTGGATA 
AAGAAAGGAA CGGATGTAGA TGTAGGGCCA TTTTTGAACT CCCTTGTGCA AGAAGGAGAA 
TGGGAAAGAG CTGCTGCTGT GGCTTTGTTC AACTTGGATA TTCGGCGAGC AATCCAAATC 
CTGAATGAAG GGGCGTCTTC AGAAAAAGGA GATCTGAATC TCAATGTGGT GGCAATGGCT 
TTATCGGGGT ATACGGACGA GAAGAACTCG CTGTGGCGGG AGATGTGCAG CACCCTGCGC 
CTGCAGCTCA ACAACCCCTA TCTGTGTGTC ATGTTTGCGT TCCTGACGAG TGAAGCGGGG 
GCATATGATG GAGTGTTGTA TGAAAACAAA GTTGCTGTAC GTGACAGAGT GGCATTCGCT 
TGTAAATTCC TTGGTGATGC TCAGTTAAAT AAATACATTG AAAAGCTGAC CAATGAAATG 
AAAGAGGCTG GAAATTTGGA GGGGATCTTG CTTACAGGCC TCACTAAAGA TGGAGTGGAC 
CTGATGGAGA GCTATGTGGA TAGAACTGGA GATGTTCAAA CAGCAAGTTA CTGCATGTTA 
CAGGGTTCAC CTTTAGATGT TCTGAAAGAT GAAAGGGTTC AATATTGGAT TGAGAATTAC 
AGAAATTTAT TAGATGCCTG GAGGTTTTGG CACAAAAGGG CAGAATTTGA TATTCATAGG 
AGTAAGCTGG ATCCCAGTTC CAAGCCTTTA GCACAGGTGT TTGTGAGTTG TAATTTTTGT 
GGCAAATCAA TCTCCTACAG CTGTTCCTCT GTGCCTCATC AGGGCAGAGG GTTTAGTCAG 
TATGGTGTGA GTGGCTCACC AACAAAATCC AAAGTCACAA GCTGCCCTGG CTGTCGGAAA 
CCCCTTCCTC GATGTGCACT TTGCCTCATT AATATGGGAA CACCTGTTTC TAGCTGTCCT 
GGTGGATCCA AATCAGATGA AAAGGTGGAC TTGAGCAAGG ACAAAAAGTT AGCTCAGTTT 
AACAACTGGT TTACCTGGTG TCACAACTGT CGGCATGGTG GGCATGCCGG ACATATGCTG 
AGTTGGTTCA GGGACCATGC AGAGTGTCCT GTATCTGCAT GCACGTGTAA ATGTATGCAG 
TTGGATACAA CAGGAAATCT GGTGCCAGCA GAGACTGTCC AGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505149.1
CDS	342..2969
Translation	MSGTKPDILWAPHQVDRFVVCDSELSLYHVESAVNSELKAGSLRLSEDSAATLLSINSDTPYMKCVAWYLNYDPECLLAVGQANGRVVLTSLGQDHNSKFKDLIGKEFVPKHARQCNTLAWNPLDSNWLAAGLDKHRADFSVLIWDICSKYTPDIVPMEKVRLSAGEAETTLLVTKPLYELGQNDASLSLCWLPRDQKLLLAGMHRNLAIFDLRNTSQKMFVNTKAVQGVTVDPYFHDRVASFYEGQVAIWDLRKFEKPVLTLTEQPKPLTKVAWCPTRTGLLATLTRDSNIIRLYDMQHTPTPIGDETEPTIIERSVQPCDNYIASFAWHPTSQNRMIVVTPNRTMSDFTVFERISLAWSPITSLMWACGRHLYECAEEESDNSLEKDIATKMRLRALSRYGLDTEQVWRNHILAGNEDPQLKSLWYTLHFMKQYTEDNDQKSPGNKGSLVYAGIKSIVKSSLGMVESSRHNWSGLDKQTDIQNLNEERILALQLCGWIKKGTDVDVGPFLNSLVQEGEWERAAAVALFNLDIRRAIQILNEGASSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNNPYLCVMFAFLTSEAGAYDGVLYENKVAVRDRVAFACKFLGDAQLNKYIEKLTNEMKEAGNLEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLDAWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSSVPHQGRGFSQYGVSGSPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGSKSDEKVDLSKDKKLAQFNNWFTWCHNCRHGGHAGHMLSWFRDHAECPVSACTCKCMQLDTTGNLVPAETVQP

Target ORF information:

RefSeq Version	XM_006505086.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus meiosis regulator for oocyte development (Mios), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505086.4

ORF Insert Sequence:

ATGAGCGGTA CCAAGCCCGA TATTTTGTGG GCACCACACC AAGTTGATAG ATTTGTTGTA 
TGTGACTCAG AATTGAGCCT TTATCATGTG GAATCGGCTG TGAATTCAGA ACTCAAAGCT 
GGATCGTTAC GTTTATCTGA AGACTCTGCA GCGACATTGC TGTCAATAAA CTCAGATACA 
CCCTATATGA AATGTGTTGC GTGGTATCTC AATTATGATC CTGAATGTCT CCTAGCAGTT 
GGACAAGCAA ATGGTCGAGT TGTACTTACA AGTCTTGGTC AAGATCATAA CTCTAAGTTC 
AAAGATTTGA TAGGAAAAGA ATTTGTTCCA AAGCATGCTC GCCAATGCAA TACCCTTGCA 
TGGAATCCAC TGGATAGTAA CTGGCTTGCT GCTGGTCTCG ACAAACACAG AGCTGACTTT 
TCAGTTCTAA TATGGGATAT CTGCAGCAAA TATACTCCTG ATATTGTTCC CATGGAAAAA 
GTGAGACTTT CAGCAGGTGA AGCTGAAACA ACATTACTAG TGACAAAACC GCTGTATGAG 
TTAGGGCAGA ATGACGCTTC TCTATCTCTG TGTTGGCTTC CACGAGACCA GAAACTTCTT 
CTTGCTGGCA TGCATCGTAA TCTAGCAATA TTTGATCTCC GGAACACAAG CCAGAAGATG 
TTTGTAAATA CAAAAGCTGT TCAAGGAGTG ACAGTAGACC CTTACTTCCA CGATCGTGTT 
GCTTCCTTCT ATGAAGGTCA GGTTGCAATA TGGGATCTTA GAAAATTTGA GAAGCCAGTT 
TTGACTTTGA CTGAACAACC AAAGCCCTTG ACAAAGGTCG CATGGTGTCC AACCAGGACA 
GGCCTGCTTG CTACTTTAAC AAGGGATAGT AATATTATTA GGCTGTATGA TATGCAGCAT 
ACACCCACTC CCATCGGGGA TGAAACCGAA CCCACAATAA TTGAAAGAAG TGTGCAGCCT 
TGTGACAATT ACATTGCTTC CTTTGCATGG CATCCAACGA GTCAAAATCG AATGATAGTT 
GTGACTCCCA ACCGAACAAT GTCTGACTTC ACTGTATTTG AAAGGATATC TCTTGCCTGG 
AGCCCAATTA CATCTTTAAT GTGGGCTTGT GGTCGCCATT TGTATGAGTG TGCAGAAGAA 
GAAAGTGATA ACTCCTTAGA AAAAGATATA GCAACAAAAA TGCGCCTTCG GGCTTTATCA 
AGGTATGGAC TTGATACAGA ACAGGTTTGG AGAAACCACA TTTTAGCTGG CAATGAAGAC 
CCACAGCTCA AGTCACTCTG GTATACTCTG CACTTTATGA AGCAATATAC AGAAGATAAT 
GATCAAAAAT CTCCAGGCAA CAAAGGATCA TTGGTTTATG CAGGAATTAA ATCAATAGTA 
AAATCATCTT TGGGAATGGT AGAAAGCAGC AGACATAATT GGAGTGGCTT GGATAAACAA 
ACTGATATTC AGAACTTAAA TGAAGAAAGG ATTTTAGCAT TACAGCTTTG TGGGTGGATA 
AAGAAAGGAA CGGATGTAGA TGTAGGGCCA TTTTTGAACT CCCTTGTGCA AGAAGGAGAA 
TGGGAAAGAG CTGCTGCTGT GGCTTTGTTC AACTTGGATA TTCGGCGAGC AATCCAAATC 
CTGAATGAAG GGGCGTCTTC AGAAAAAGGA GATCTGAATC TCAATGTGGT GGCAATGGCT 
TTATCGGGGT ATACGGACGA GAAGAACTCG CTGTGGCGGG AGATGTGCAG CACCCTGCGC 
CTGCAGCTCA ACAACCCCTA TCTGTGTGTC ATGTTTGCGT TCCTGACGAG TGAAGCGGGG 
GCATATGATG GAGTGTTGTA TGAAAACAAA GTTGCTGTAC GTGACAGAGT GGCATTCGCT 
TGTAAATTCC TTGGTGATGC TCAGTTAAAT AAATACATTG AAAAGCTGAC CAATGAAATG 
AAAGAGGCTG GAAATTTGGA GGGGATCTTG CTTACAGGCC TCACTAAAGA TGGAGTGGAC 
CTGATGGAGA GCTATGTGGA TAGAACTGGA GATGTTCAAA CAGCAAGTTA CTGCATGTTA 
CAGGGTTCAC CTTTAGATGT TCTGAAAGAT GAAAGGGTTC AATATTGGAT TGAGAATTAC 
AGAAATTTAT TAGATGCCTG GAGGTTTTGG CACAAAAGGG CAGAATTTGA TATTCATAGG 
AGTAAGCTGG ATCCCAGTTC CAAGCCTTTA GCACAGGTGT TTGTGAGTTG TAATTTTTGT 
GGCAAATCAA TCTCCTACAG CTGTTCCTCT GTGCCTCATC AGGGCAGAGG GTTTAGTCAG 
TATGGTGTGA GTGGCTCACC AACAAAATCC AAAGTCACAA GCTGCCCTGG CTGTCGGAAA 
CCCCTTCCTC GATGTGCACT TTGCCTCATT AATATGGGAA CACCTGTTTC TAGCTGTCCT 
GGTGGATCCA AATCAGATGA AAAGGTGGAC TTGAGCAAGG ACAAAAAGTT AGCTCAGTTT 
AACAACTGGT TTACCTGGTG TCACAACTGT CGGCATGGTG GGCATGCCGG ACATATGCTG 
AGTTGGTTCA GGGACCATGC AGAGTGTCCT GTATCTGCAT GCACGTGTAA ATGTATGCAG 
TTGGATACAA CAGGAAATCT GGTGCCAGCA GAGACTGTCC AGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)
Lumayag S,Haldin CE,Corbett NJ,Wahlin KJ,Cowan C,Turturro S,Larsen PE,Kovacs B,Witmer PD,Valle D,Zack DJ,Nicholson DA,Xu S

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)
Nishimura M,Yokoi N,Miki T,Horikawa Y,Yoshioka H,Takeda J,Ohara O,Seino S

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Mios cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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