Greb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Greb1
Entrez Gene ID	268527
Full Name	gene regulated by estrogen in breast cancer protein
Synonyms	5730583K22Rik,9130004E13,AF180470,AU023194,mKIAA0575
General protein information	Preferred Names gene regulated by estrogen in breast cancer protein Names protein GREB1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 A1.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006515104.3	XP_006515167.1	protein GREB1 isoform X6
XM_006515107.3	XP_006515170.1	protein GREB1 isoform X9
XM_006515093.3	XP_006515156.1	protein GREB1 isoform X1
XM_006515094.3	XP_006515157.1	protein GREB1 isoform X1
XM_030246728.1	XP_030102588.1	protein GREB1 isoform X5
XM_006515103.4	XP_006515166.1	protein GREB1 isoform X6
NM_015764.4	NP_056579.2	protein GREB1 isoform 1
XM_006515105.3	XP_006515168.1	protein GREB1 isoform X7
XM_006515097.3	XP_006515160.1	protein GREB1 isoform X1
XM_006515098.3	XP_006515161.1	protein GREB1 isoform X1
XM_006515104.4	XP_006515167.1	protein GREB1 isoform X7
XM_006515105.4	XP_006515168.1	protein GREB1 isoform X8
XM_006515093.2	XP_006515156.1	protein GREB1 isoform X1
XM_006515098.2	XP_006515161.1	protein GREB1 isoform X1
XM_006515106.4	XP_006515169.1	protein GREB1 isoform X9
XM_006515107.4	XP_006515170.1	protein GREB1 isoform X10
XM_006515099.2	XP_006515162.1	protein GREB1 isoform X2
XM_006515103.3	XP_006515166.1	protein GREB1 isoform X5
XM_006515095.3	XP_006515158.1	protein GREB1 isoform X1
XM_006515096.3	XP_006515159.1	protein GREB1 isoform X1
XM_006515099.3	XP_006515162.1	protein GREB1 isoform X2
XM_006515101.4	XP_006515164.1	protein GREB1 isoform X4
NM_001252071.1	NP_001239000.1	protein GREB1 isoform 2
XM_006515096.2	XP_006515159.1	protein GREB1 isoform X1
XM_006515100.2	XP_006515163.1	protein GREB1 isoform X3
XM_006515101.3	XP_006515164.1	protein GREB1 isoform X4
XM_006515106.3	XP_006515169.1	protein GREB1 isoform X8
XM_006515100.3	XP_006515163.1	protein GREB1 isoform X3

Pathways from BioSystems

Homologies

	GREB1	XP_001089374.1
Pan troglodytes (chimpanzee)	GREB1	XP_515304.3
Bos taurus (cattle)	GREB1	NP_001192560.1
Danio rerio (zebrafish)	greb1	XP_005158874.1
Homo sapiens (human)	GREB1	NP_055483.2
Canis lupus familiaris (dog)	GREB1	XP_005630186.1
Mus musculus (house mouse)	Greb1	NP_056579.2
Rattus norvegicus (Norway rat)	Greb1	XP_006239984.1
Gallus gallus (chicken)	GREB1	XP_004935825.1
Xenopus tropicalis (tropical clawed frog)	greb1	XP_002942230.2

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in GREB1L Cause Bilateral Kidney Agenesis in Humans and Mice.
De Tomasi L, David P, Humbert C, Silbermann F, Arrondel C, Tores F, Fouquet S, Desgrange A, Niel O, Bole-Feysot C, Nitschk? P, Roume J, Cordier MP, Pietrement C, Isidor B, Khau Van Kien P, Gonzales M, Saint-Frison MH, Martinovic J, Novo R, Piard J, Cabrol C, Verma IC, Puri R, Journel H, Aziza J, Gavard L, Said-Menthon MH, Heidet L, Saunier S, Jeanpierre C
American journal of human genetics101(5)803-814(2017 Nov)

Growth regulation by estrogen in breast cancer 1 (GREB1) is a novel progesterone-responsive gene required for human endometrial stromal decidualization.
Camden AJ, Szwarc MM, Chadchan SB, DeMayo FJ, O'Malley BW, Lydon JP, Kommagani R
Molecular human reproduction23(9)646-653(2017 Sep)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A significant association between GREB1L variants and BKA.
Title: Mutations in GREB1L Cause Bilateral Kidney Agenesis in Humans and Mice.

The following Greb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Greb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu47324	XM_006515103.4 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price
OMu47325	XM_006515104.4 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price
OMu47326	XM_006515105.4 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price
OMu47327	XM_006515106.4 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price
OMu47328	XM_006515107.4 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price
OMu74319	XM_030246728.1 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	Quote Price
OMu04813	XM_006515100.3 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
OMu10233	XM_006515096.3 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
OMu10233	XM_006515093.3 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
OMu10233	XM_006515098.3 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
OMu47321	XM_006515099.3 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
OMu47322	XM_006515101.4 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
OMu04813	NM_001252071.1 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
OMu10233	NM_015764.4 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
OMu10233	XM_006515095.3 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
OMu10233	XM_006515094.3 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
OMu10233	XM_006515097.3 Latest version!	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
View Old Accession Versions >>
OMu47325	XM_006515104.3	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	Quote Price
OMu47326	XM_006515105.3	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	Quote Price
OMu10233	XM_006515093.2	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
OMu10233	XM_006515098.2	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
OMu47328	XM_006515107.3	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	Quote Price
OMu47324	XM_006515103.3	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	Quote Price
OMu47321	XM_006515099.2	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
OMu10233	XM_006515096.2	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
OMu47322	XM_006515101.3	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
OMu47327	XM_006515106.3	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	Quote Price
OMu04813	XM_006515100.2	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Greb1 ( XM_006515103.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515166.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu47324
Clone ID Related Accession (Same CDS sequence)	XM_006515103.4 , XM_006515103.3
Accession Version	XM_006515103.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515103.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG CAGTGCTGTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515166.1
CDS	1058..3322
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQQCCS

Target ORF information:

RefSeq Version	XM_006515103.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515103.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG CAGTGCTGTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515104.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515167.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu47325
Clone ID Related Accession (Same CDS sequence)	XM_006515104.3 , XM_006515104.4
Accession Version	XM_006515104.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515104.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG TGCTGTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515167.1
CDS	1058..3319
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQCCS

Target ORF information:

RefSeq Version	XM_006515104.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515104.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG TGCTGTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515105.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515168.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X14, mRNA.

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CloneID	OMu47326
Clone ID Related Accession (Same CDS sequence)	XM_006515105.3 , XM_006515105.4
Accession Version	XM_006515105.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGGAAAAT CAAGGCCATG TCTCTTCCTC 
ATTCACTGCT TCCTCTACCA AGAAGACAGC TGTGCTGGAT GCCAGTGGGG TCCCGGTGTG 
CACAAGTTAC CACCAGGAGC CACGGGGCGT CCGGCCCTTT CAGCTGGCGG TGGCACAGAA 
GCTCCTTTCC CACGTGTGCT CCATCGCAGA TTCCAGCACC CAGAATCTGG ACCTGGGGTC 
CTTCGAGAAG GTGGACTTTC TCATTTGCAT TCCACCGTCA GAGGTGACCT ACCAGCAGAC 
CGTGTTCCAC GTCTGGCATT CAGGGGTTCT GCTGGAGCTG GGCCTGGAGA AAGAGCCTGT 
GACCAAGCAG AGGGCGGAGC AACACGTTCT GAAACTAGAC ACGGAGGCCC AGCAGTGCTG 
TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515168.1
CDS	1058..3319
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPGKSRPCLFLIHCFLYQEDSCAGCQWGPGVHKLPPGATGRPALSAGGGTEAPFPRVLHRRFQHPESGPGVLREGGLSHLHSTVRGDLPADRVPRLAFRGSAGAGPGERACDQAEGGATRSETRHGGPAVLFITSTLKVTHLWG

Target ORF information:

RefSeq Version	XM_006515105.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515105.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGGAAAAT CAAGGCCATG TCTCTTCCTC 
ATTCACTGCT TCCTCTACCA AGAAGACAGC TGTGCTGGAT GCCAGTGGGG TCCCGGTGTG 
CACAAGTTAC CACCAGGAGC CACGGGGCGT CCGGCCCTTT CAGCTGGCGG TGGCACAGAA 
GCTCCTTTCC CACGTGTGCT CCATCGCAGA TTCCAGCACC CAGAATCTGG ACCTGGGGTC 
CTTCGAGAAG GTGGACTTTC TCATTTGCAT TCCACCGTCA GAGGTGACCT ACCAGCAGAC 
CGTGTTCCAC GTCTGGCATT CAGGGGTTCT GCTGGAGCTG GGCCTGGAGA AAGAGCCTGT 
GACCAAGCAG AGGGCGGAGC AACACGTTCT GAAACTAGAC ACGGAGGCCC AGCAGTGCTG 
TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515106.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515169.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OMu47327
Clone ID Related Accession (Same CDS sequence)	XM_006515106.4 , XM_006515106.3
Accession Version	XM_006515106.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515106.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGGAAAAT CAAGGCCATG TCTCTTCCTC 
ATTCACTGCT TCCTCTACCA AGAAGACAGC TGTGCTGGAT GCCAGTGGGG TCCCGGTGTG 
CACAAGTTAC CACCAGGAGC CACGGGGCGT CCGGCCCTTT CAGCTGGCGG TGGCACAGAA 
GCTCCTTTCC CACGTGTGCT CCATCGCAGA TTCCAGCACC CAGAATCTGG ACCTGGGGTC 
CTTCGAGAAG GTGGACTTTC TCATTTGCAT TCCACCGTCA GAGGTGACCT ACCAGCAGAC 
CGTGTTCCAC GTCTGGCATT CAGGGGTTCT GCTGGAGCTG GGCCTGGAGA AAGAGCCTGT 
GACCAAGCAG AGGGCGGAGC AACACGTTCT GAAACTAGAC ACGGAGGCCC AGTGCTGTTC 
ATAACATCTA CTCTCAAAGT GACCCATCTG TGGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515169.1
CDS	1058..3316
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPGKSRPCLFLIHCFLYQEDSCAGCQWGPGVHKLPPGATGRPALSAGGGTEAPFPRVLHRRFQHPESGPGVLREGGLSHLHSTVRGDLPADRVPRLAFRGSAGAGPGERACDQAEGGATRSETRHGGPVLFITSTLKVTHLWG

Target ORF information:

RefSeq Version	XM_006515106.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515106.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGGAAAAT CAAGGCCATG TCTCTTCCTC 
ATTCACTGCT TCCTCTACCA AGAAGACAGC TGTGCTGGAT GCCAGTGGGG TCCCGGTGTG 
CACAAGTTAC CACCAGGAGC CACGGGGCGT CCGGCCCTTT CAGCTGGCGG TGGCACAGAA 
GCTCCTTTCC CACGTGTGCT CCATCGCAGA TTCCAGCACC CAGAATCTGG ACCTGGGGTC 
CTTCGAGAAG GTGGACTTTC TCATTTGCAT TCCACCGTCA GAGGTGACCT ACCAGCAGAC 
CGTGTTCCAC GTCTGGCATT CAGGGGTTCT GCTGGAGCTG GGCCTGGAGA AAGAGCCTGT 
GACCAAGCAG AGGGCGGAGC AACACGTTCT GAAACTAGAC ACGGAGGCCC AGTGCTGTTC 
ATAACATCTA CTCTCAAAGT GACCCATCTG TGGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515107.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515170.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X18, mRNA.

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CloneID	OMu47328
Clone ID Related Accession (Same CDS sequence)	XM_006515107.3 , XM_006515107.4
Accession Version	XM_006515107.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515107.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGGAAAAT CAAGGCCATG TCTCTTCCTC 
ATTCACTGCT TCCTCTACCA AGAAGACAGC TGTGCTGGAT GCCAGTGGGG TCCCGGTGTG 
CACAAATTCC AGCACCCAGA ATCTGGACCT GGGGTCCTTC GAGAAGGTGG ACTTTCTCAT 
TTGCATTCCA CCGTCAGAGG TGACCTACCA GCAGACCGTG TTCCACGTCT GGCATTCAGG 
GGTTCTGCTG GAGCTGGGCC TGGAGAAAGA GCCTGTGACC AAGCAGAGGG CGGAGCAACA 
CGTTCTGAAA CTAGACACGG AGGCCCAGCA GTGCTGTTCA TAACATCTAC TCTCAAAGTG 
ACCCATCTGT GGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515170.1
CDS	1059..3236
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPGKSRPCLFLIHCFLYQEDSCAGCQWGPGVHKFQHPESGPGVLREGGLSHLHSTVRGDLPADRVPRLAFRGSAGAGPGERACDQAEGGATRSETRHGGPAVLFITSTLKVTHLWG

Target ORF information:

RefSeq Version	XM_006515107.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515107.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGGAAAAT CAAGGCCATG TCTCTTCCTC 
ATTCACTGCT TCCTCTACCA AGAAGACAGC TGTGCTGGAT GCCAGTGGGG TCCCGGTGTG 
CACAAATTCC AGCACCCAGA ATCTGGACCT GGGGTCCTTC GAGAAGGTGG ACTTTCTCAT 
TTGCATTCCA CCGTCAGAGG TGACCTACCA GCAGACCGTG TTCCACGTCT GGCATTCAGG 
GGTTCTGCTG GAGCTGGGCC TGGAGAAAGA GCCTGTGACC AAGCAGAGGG CGGAGCAACA 
CGTTCTGAAA CTAGACACGG AGGCCCAGCA GTGCTGTTCA TAACATCTAC TCTCAAAGTG 
ACCCATCTGT GGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515103.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515166.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu47324
Clone ID Related Accession (Same CDS sequence)	XM_006515103.4 , XM_006515103.3
Accession Version	XM_006515103.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515103.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG CAGTGCTGTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515166.1
CDS	1057..3321
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQQCCS

Target ORF information:

RefSeq Version	XM_006515103.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515103.4

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG CAGTGCTGTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515104.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515167.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu47325
Clone ID Related Accession (Same CDS sequence)	XM_006515104.3 , XM_006515104.4
Accession Version	XM_006515104.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515104.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG TGCTGTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515167.1
CDS	1057..3318
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQCCS

Target ORF information:

RefSeq Version	XM_006515104.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515104.4

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG TGCTGTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515105.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515168.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X15, mRNA.

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CloneID	OMu47326
Clone ID Related Accession (Same CDS sequence)	XM_006515105.3 , XM_006515105.4
Accession Version	XM_006515105.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515105.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGGAAAAT CAAGGCCATG TCTCTTCCTC 
ATTCACTGCT TCCTCTACCA AGAAGACAGC TGTGCTGGAT GCCAGTGGGG TCCCGGTGTG 
CACAAGTTAC CACCAGGAGC CACGGGGCGT CCGGCCCTTT CAGCTGGCGG TGGCACAGAA 
GCTCCTTTCC CACGTGTGCT CCATCGCAGA TTCCAGCACC CAGAATCTGG ACCTGGGGTC 
CTTCGAGAAG GTGGACTTTC TCATTTGCAT TCCACCGTCA GAGGTGACCT ACCAGCAGAC 
CGTGTTCCAC GTCTGGCATT CAGGGGTTCT GCTGGAGCTG GGCCTGGAGA AAGAGCCTGT 
GACCAAGCAG AGGGCGGAGC AACACGTTCT GAAACTAGAC ACGGAGGCCC AGCAGTGCTG 
TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515168.1
CDS	1057..3318
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPGKSRPCLFLIHCFLYQEDSCAGCQWGPGVHKLPPGATGRPALSAGGGTEAPFPRVLHRRFQHPESGPGVLREGGLSHLHSTVRGDLPADRVPRLAFRGSAGAGPGERACDQAEGGATRSETRHGGPAVLFITSTLKVTHLWG

Target ORF information:

RefSeq Version	XM_006515105.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515105.4

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGGAAAAT CAAGGCCATG TCTCTTCCTC 
ATTCACTGCT TCCTCTACCA AGAAGACAGC TGTGCTGGAT GCCAGTGGGG TCCCGGTGTG 
CACAAGTTAC CACCAGGAGC CACGGGGCGT CCGGCCCTTT CAGCTGGCGG TGGCACAGAA 
GCTCCTTTCC CACGTGTGCT CCATCGCAGA TTCCAGCACC CAGAATCTGG ACCTGGGGTC 
CTTCGAGAAG GTGGACTTTC TCATTTGCAT TCCACCGTCA GAGGTGACCT ACCAGCAGAC 
CGTGTTCCAC GTCTGGCATT CAGGGGTTCT GCTGGAGCTG GGCCTGGAGA AAGAGCCTGT 
GACCAAGCAG AGGGCGGAGC AACACGTTCT GAAACTAGAC ACGGAGGCCC AGCAGTGCTG 
TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515106.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515169.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu47327
Clone ID Related Accession (Same CDS sequence)	XM_006515106.4 , XM_006515106.3
Accession Version	XM_006515106.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515106.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGGAAAAT CAAGGCCATG TCTCTTCCTC 
ATTCACTGCT TCCTCTACCA AGAAGACAGC TGTGCTGGAT GCCAGTGGGG TCCCGGTGTG 
CACAAGTTAC CACCAGGAGC CACGGGGCGT CCGGCCCTTT CAGCTGGCGG TGGCACAGAA 
GCTCCTTTCC CACGTGTGCT CCATCGCAGA TTCCAGCACC CAGAATCTGG ACCTGGGGTC 
CTTCGAGAAG GTGGACTTTC TCATTTGCAT TCCACCGTCA GAGGTGACCT ACCAGCAGAC 
CGTGTTCCAC GTCTGGCATT CAGGGGTTCT GCTGGAGCTG GGCCTGGAGA AAGAGCCTGT 
GACCAAGCAG AGGGCGGAGC AACACGTTCT GAAACTAGAC ACGGAGGCCC AGTGCTGTTC 
ATAACATCTA CTCTCAAAGT GACCCATCTG TGGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515169.1
CDS	1057..3315
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPGKSRPCLFLIHCFLYQEDSCAGCQWGPGVHKLPPGATGRPALSAGGGTEAPFPRVLHRRFQHPESGPGVLREGGLSHLHSTVRGDLPADRVPRLAFRGSAGAGPGERACDQAEGGATRSETRHGGPVLFITSTLKVTHLWG

Target ORF information:

RefSeq Version	XM_006515106.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515106.4

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGGAAAAT CAAGGCCATG TCTCTTCCTC 
ATTCACTGCT TCCTCTACCA AGAAGACAGC TGTGCTGGAT GCCAGTGGGG TCCCGGTGTG 
CACAAGTTAC CACCAGGAGC CACGGGGCGT CCGGCCCTTT CAGCTGGCGG TGGCACAGAA 
GCTCCTTTCC CACGTGTGCT CCATCGCAGA TTCCAGCACC CAGAATCTGG ACCTGGGGTC 
CTTCGAGAAG GTGGACTTTC TCATTTGCAT TCCACCGTCA GAGGTGACCT ACCAGCAGAC 
CGTGTTCCAC GTCTGGCATT CAGGGGTTCT GCTGGAGCTG GGCCTGGAGA AAGAGCCTGT 
GACCAAGCAG AGGGCGGAGC AACACGTTCT GAAACTAGAC ACGGAGGCCC AGTGCTGTTC 
ATAACATCTA CTCTCAAAGT GACCCATCTG TGGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515107.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515170.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X19, mRNA.

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CloneID	OMu47328
Clone ID Related Accession (Same CDS sequence)	XM_006515107.3 , XM_006515107.4
Accession Version	XM_006515107.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515107.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGGAAAAT CAAGGCCATG TCTCTTCCTC 
ATTCACTGCT TCCTCTACCA AGAAGACAGC TGTGCTGGAT GCCAGTGGGG TCCCGGTGTG 
CACAAATTCC AGCACCCAGA ATCTGGACCT GGGGTCCTTC GAGAAGGTGG ACTTTCTCAT 
TTGCATTCCA CCGTCAGAGG TGACCTACCA GCAGACCGTG TTCCACGTCT GGCATTCAGG 
GGTTCTGCTG GAGCTGGGCC TGGAGAAAGA GCCTGTGACC AAGCAGAGGG CGGAGCAACA 
CGTTCTGAAA CTAGACACGG AGGCCCAGCA GTGCTGTTCA TAACATCTAC TCTCAAAGTG 
ACCCATCTGT GGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515170.1
CDS	1057..3234
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPGKSRPCLFLIHCFLYQEDSCAGCQWGPGVHKFQHPESGPGVLREGGLSHLHSTVRGDLPADRVPRLAFRGSAGAGPGERACDQAEGGATRSETRHGGPAVLFITSTLKVTHLWG

Target ORF information:

RefSeq Version	XM_006515107.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515107.4

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGGAAAAT CAAGGCCATG TCTCTTCCTC 
ATTCACTGCT TCCTCTACCA AGAAGACAGC TGTGCTGGAT GCCAGTGGGG TCCCGGTGTG 
CACAAATTCC AGCACCCAGA ATCTGGACCT GGGGTCCTTC GAGAAGGTGG ACTTTCTCAT 
TTGCATTCCA CCGTCAGAGG TGACCTACCA GCAGACCGTG TTCCACGTCT GGCATTCAGG 
GGTTCTGCTG GAGCTGGGCC TGGAGAAAGA GCCTGTGACC AAGCAGAGGG CGGAGCAACA 
CGTTCTGAAA CTAGACACGG AGGCCCAGCA GTGCTGTTCA TAACATCTAC TCTCAAAGTG 
ACCCATCTGT GGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_030246728.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102588.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X12, mRNA.

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CloneID	OMu74319
Clone ID Related Accession (Same CDS sequence)	XM_030246728.1
Accession Version	XM_030246728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCACTG CATTGGGGAA AAGGTTCCCC CGCCTGCACA GCGCGGTCAT CAGGACCTTT 
GTTCTTGTGC AGCACTATGC GGCCGCCATG ATGGCCGTGA GTGGCCTCCC GCAGATGAAG 
AACCATACAT CAGTGGAGAC CCTAGAGATC ACCCAGAACC TCCTCAACTC CCCGAAGCAG 
TGCCCTTGTG GCCACGGGCT CATGGTCCTG CTGCGCGTGC CCTGTTCACC CCTGGCAGCC 
GTGGCCTATG AGCGACTGGC CCACGTGCGG GCTCGCTTGG CTCTGGAGGA ACACTTTGAG 
ATCATCCTGG GCCACCCCAG CTCCGGTATC ACTGTAGGCA AGCACTTTGT GAAGCAGCTC 
AAGATGTGGC AGAAAATTGA GGATGCAGAA TGGAGGCCTC AGACGTACTT AGAGTTGGAG 
GGCCTGCCCT GCATCCTGAT CTTCAGTGGG ATGGACCCTC ATGGGGAGTC ACTGCCCCGG 
TCTCTGAGGT ACTGTGACCT GCGTCTAATA AACTCCTCCT GCCTGGTGAG GACAGCCCTG 
GAGCAGGAGC TGGGTCTGGC TGCCTACTTT GTTAGCAATG ATATCCCCTT GGAGAAGGGG 
CCCAAGAACG AGGCCTTGGA GAGTGATGGG GAGAAACTGA GCAGCACCGA TGAAGACGAG 
GAGGCGGGGA CAGAAGGGTG TTTTGAAGCA GGCTCTACGT CAGAGCAGAG GGGCCCTGTG 
AAGAGGGAAC GCTCCCACTC CCACGACTCT GCATCGTCAT CCCTCTCCTC CAGAGCATCT 
GGCTCGGTGC TCTATGGTGA GTCCCTGGCC CAGCCCTCAG GACCTCCTCA GGGAGAGCTT 
ACCAGATCAC CTCCATCCTG TGGCCCTGCA GAGGAAGGCA GGGCCCCTGG GGAAATCCAG 
CGCCTCCGAG TGAGTCAGGG GTCTACAGTC ATCAGTAGGC ACAGCCCTGG CCTGGTCCCA 
CAGCCTGATT CTAGCCTCAG GACTGGTCGG AGGAGCTTGC AGGTGCCAGC CGCCCCGTCC 
TCCCAGCTGT CCTCCTCCTC GGGCTCATCA TCCACATGTG CGGTGCCCAC TGCCAATGTG 
CTGGTCCTTC AAGCCTCTCA GTGCTCCATG GCCAAGGCCT GCCGGCAGCC ACCCATCGTC 
TTCCTGCCGA AGCTTGTGTA CGACATGCTC CTGTCCACCG ACAGCAGCGG CTTGCCCAAG 
TCTGCCTCCC TCCTGCCTTC CCCTTCAGTC ATGTGGACCA GCTCCTTCCG CCCCCTGCTC 
AGCAAGATGA TGACATCAAC TGAGCAGTCT CTCTACTACC GGCAGTGGAC AGTGCCCCGG 
CCCAGCCACA TGGACTACGG CAACAGAGCT GAGGGCCGTG TGGACAGCTT CCACCCCCGC 
AGGCTGCTGC TCAGTGGGCC ACCTCAGATT GGGAAGACAG GTGCCTACCT GCAGTTCCTC 
AGTATCTTGT CCAGGATGCT GATTCGGCTG ACAGAAGTGG ATGTTTACGA CGAAGAAGAG 
ATCAATACCA GCTTCCGAGA GGAATCGGAG TGGCGTTACC TCCAGCTGGC CGACCCCTGG 
CCAGACCTGG AGCTCTTCCA GAAGATGCCT TTTGATTACA TCATTCATGA CCCGAAGTAT 
GAGGACGCCA GTCTGATTTG TTCACACCCT CAGACCATCA AGAGTGAAGA CAGAGGGATG 
TCCAGGAAGC CGGAGGACCT GTATGTGCGG CGTCAGACGG CACGTATGAG ACTGTCCAAG 
TATGCAGCAT ACAACACGTA CCACCACTGT GAGCAGTGCC AGCAGTACAT GGGCTTCCAC 
CCCCACTACC AGCTCTCTGA GTCCACCCTG CACGTCTTTG CCTTCTCTTG CTCCATGCTA 
GGAGAGGAGG TCCAACTTCA CTTCATCATC CCCAAGTCCA AGGAGCACCA CTTTGTTTTC 
AGCCAACCCG GAGGCCAGCT GGAGAGCATG CGCTTGCCCC TCGTCACAGA CAAGAGTCAT 
GAACACATCA AAAGCCCAAC CTTCACACCG ACAACGGGCC GGCACGAGCA CGGGCTCTTT 
AATTTGTACC ATGCGATGGA TGGAGCCAAC CACCTACACG TGCTGGTGGT CAAGGAGTAC 
GAAATGGCCA TTTATAAGAA ATACTGGCCC AACCACATCA TGCTTGTCCT CCCAAGCATC 
TTTAACAGTG CTGGTGTCGG GGCTGCCCAC TTCCTGATCA AAGAGCTTTG TTACCACAAC 
CTGGAGCTGG AGCGGAACCG GCAGGAGGAG CTGGGGGTCA AGCCTCAGGA CGTCTGGCCC 
TTCATCGTCA TTGCTGATGA CTCCTGTGTG ATGTGGAATG TAGCAGATGT GGACTGTGCC 
GGAGAACGGA GCAGGGAGTT TTCCTGGTCG GAGAGGAATG TCTCTCTGAA ATACATCATG 
CTACACATTG AGGCGTCCCC TAACATCACA CACTATGCCC TGCTCGGCAT GCGGAAGTGG 
GCCAGCAAGA CCCGGGGCCG AGAGGTACAG GAGCCTTTTT CACGATGTCA TGTGCACGAC 
TTCATCATCC TCAACGTGGA CTTGACCCAG AATGTACAGT ACAACCAGAA CCGGTTTACA 
TGTGACGATG TAGACTTCAA CCTCCGTGTG CACAGTGCTG GCCTCCTGCT CTGCCGGTTC 
AATCGCTTCA GTGTCATGAA GAAGCAGATT GCAGTGGGTG GGCACAGGTC TTTCCACATC 
ACATCCAAGG TATCTGACAG CTCTGTGGCC ATTGTGCCGT CCCAGTACAT CTGTGCCCCC 
GACAGTAAGC ATACGTTCCT GGCAGCTCCC GCCCAGCTCC TGCTGGAGAA ATTCCTCCAA 
TACCATAGCC ATCGCTTCTT CCCGCTGTCT CTGAAGAATC ACAGCCACCC CGTGTTGTCT 
GTAGACTGCT ACCTGAACCT GGGACCTCAG ATTTCTGTTT GTTATGTGAG CTCCAGGCCC 
CACTCCTTAA ACATCAGCTG CTCTGACATG GTGTTCAGCG GGCTCCTGCT GTACCTCTGT 
GACTCTTTTG TGGGGGCTAG CTTCTTGAAG AAGTTCCATT TTCTGAAAGG AGCCACCTTG 
TGTGTGATCT GCCAAGACCG GAACTCACTT CGCCAGACGG TGGTCCGCCT GGAGCTAGAA 
GATGAGTGGC AGTTCCGGCT GCGGGATGAG TTCCAGACAG CCAATGCCAA GGAAGACAGG 
CCGCTCTTTT TCCTCACCGC ACGGCACATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102588.1
CDS	908..4120
Translation	MVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_030246728.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246728.1

ORF Insert Sequence:

ATGGTCACTG CATTGGGGAA AAGGTTCCCC CGCCTGCACA GCGCGGTCAT CAGGACCTTT 
GTTCTTGTGC AGCACTATGC GGCCGCCATG ATGGCCGTGA GTGGCCTCCC GCAGATGAAG 
AACCATACAT CAGTGGAGAC CCTAGAGATC ACCCAGAACC TCCTCAACTC CCCGAAGCAG 
TGCCCTTGTG GCCACGGGCT CATGGTCCTG CTGCGCGTGC CCTGTTCACC CCTGGCAGCC 
GTGGCCTATG AGCGACTGGC CCACGTGCGG GCTCGCTTGG CTCTGGAGGA ACACTTTGAG 
ATCATCCTGG GCCACCCCAG CTCCGGTATC ACTGTAGGCA AGCACTTTGT GAAGCAGCTC 
AAGATGTGGC AGAAAATTGA GGATGCAGAA TGGAGGCCTC AGACGTACTT AGAGTTGGAG 
GGCCTGCCCT GCATCCTGAT CTTCAGTGGG ATGGACCCTC ATGGGGAGTC ACTGCCCCGG 
TCTCTGAGGT ACTGTGACCT GCGTCTAATA AACTCCTCCT GCCTGGTGAG GACAGCCCTG 
GAGCAGGAGC TGGGTCTGGC TGCCTACTTT GTTAGCAATG ATATCCCCTT GGAGAAGGGG 
CCCAAGAACG AGGCCTTGGA GAGTGATGGG GAGAAACTGA GCAGCACCGA TGAAGACGAG 
GAGGCGGGGA CAGAAGGGTG TTTTGAAGCA GGCTCTACGT CAGAGCAGAG GGGCCCTGTG 
AAGAGGGAAC GCTCCCACTC CCACGACTCT GCATCGTCAT CCCTCTCCTC CAGAGCATCT 
GGCTCGGTGC TCTATGGTGA GTCCCTGGCC CAGCCCTCAG GACCTCCTCA GGGAGAGCTT 
ACCAGATCAC CTCCATCCTG TGGCCCTGCA GAGGAAGGCA GGGCCCCTGG GGAAATCCAG 
CGCCTCCGAG TGAGTCAGGG GTCTACAGTC ATCAGTAGGC ACAGCCCTGG CCTGGTCCCA 
CAGCCTGATT CTAGCCTCAG GACTGGTCGG AGGAGCTTGC AGGTGCCAGC CGCCCCGTCC 
TCCCAGCTGT CCTCCTCCTC GGGCTCATCA TCCACATGTG CGGTGCCCAC TGCCAATGTG 
CTGGTCCTTC AAGCCTCTCA GTGCTCCATG GCCAAGGCCT GCCGGCAGCC ACCCATCGTC 
TTCCTGCCGA AGCTTGTGTA CGACATGCTC CTGTCCACCG ACAGCAGCGG CTTGCCCAAG 
TCTGCCTCCC TCCTGCCTTC CCCTTCAGTC ATGTGGACCA GCTCCTTCCG CCCCCTGCTC 
AGCAAGATGA TGACATCAAC TGAGCAGTCT CTCTACTACC GGCAGTGGAC AGTGCCCCGG 
CCCAGCCACA TGGACTACGG CAACAGAGCT GAGGGCCGTG TGGACAGCTT CCACCCCCGC 
AGGCTGCTGC TCAGTGGGCC ACCTCAGATT GGGAAGACAG GTGCCTACCT GCAGTTCCTC 
AGTATCTTGT CCAGGATGCT GATTCGGCTG ACAGAAGTGG ATGTTTACGA CGAAGAAGAG 
ATCAATACCA GCTTCCGAGA GGAATCGGAG TGGCGTTACC TCCAGCTGGC CGACCCCTGG 
CCAGACCTGG AGCTCTTCCA GAAGATGCCT TTTGATTACA TCATTCATGA CCCGAAGTAT 
GAGGACGCCA GTCTGATTTG TTCACACCCT CAGACCATCA AGAGTGAAGA CAGAGGGATG 
TCCAGGAAGC CGGAGGACCT GTATGTGCGG CGTCAGACGG CACGTATGAG ACTGTCCAAG 
TATGCAGCAT ACAACACGTA CCACCACTGT GAGCAGTGCC AGCAGTACAT GGGCTTCCAC 
CCCCACTACC AGCTCTCTGA GTCCACCCTG CACGTCTTTG CCTTCTCTTG CTCCATGCTA 
GGAGAGGAGG TCCAACTTCA CTTCATCATC CCCAAGTCCA AGGAGCACCA CTTTGTTTTC 
AGCCAACCCG GAGGCCAGCT GGAGAGCATG CGCTTGCCCC TCGTCACAGA CAAGAGTCAT 
GAACACATCA AAAGCCCAAC CTTCACACCG ACAACGGGCC GGCACGAGCA CGGGCTCTTT 
AATTTGTACC ATGCGATGGA TGGAGCCAAC CACCTACACG TGCTGGTGGT CAAGGAGTAC 
GAAATGGCCA TTTATAAGAA ATACTGGCCC AACCACATCA TGCTTGTCCT CCCAAGCATC 
TTTAACAGTG CTGGTGTCGG GGCTGCCCAC TTCCTGATCA AAGAGCTTTG TTACCACAAC 
CTGGAGCTGG AGCGGAACCG GCAGGAGGAG CTGGGGGTCA AGCCTCAGGA CGTCTGGCCC 
TTCATCGTCA TTGCTGATGA CTCCTGTGTG ATGTGGAATG TAGCAGATGT GGACTGTGCC 
GGAGAACGGA GCAGGGAGTT TTCCTGGTCG GAGAGGAATG TCTCTCTGAA ATACATCATG 
CTACACATTG AGGCGTCCCC TAACATCACA CACTATGCCC TGCTCGGCAT GCGGAAGTGG 
GCCAGCAAGA CCCGGGGCCG AGAGGTACAG GAGCCTTTTT CACGATGTCA TGTGCACGAC 
TTCATCATCC TCAACGTGGA CTTGACCCAG AATGTACAGT ACAACCAGAA CCGGTTTACA 
TGTGACGATG TAGACTTCAA CCTCCGTGTG CACAGTGCTG GCCTCCTGCT CTGCCGGTTC 
AATCGCTTCA GTGTCATGAA GAAGCAGATT GCAGTGGGTG GGCACAGGTC TTTCCACATC 
ACATCCAAGG TATCTGACAG CTCTGTGGCC ATTGTGCCGT CCCAGTACAT CTGTGCCCCC 
GACAGTAAGC ATACGTTCCT GGCAGCTCCC GCCCAGCTCC TGCTGGAGAA ATTCCTCCAA 
TACCATAGCC ATCGCTTCTT CCCGCTGTCT CTGAAGAATC ACAGCCACCC CGTGTTGTCT 
GTAGACTGCT ACCTGAACCT GGGACCTCAG ATTTCTGTTT GTTATGTGAG CTCCAGGCCC 
CACTCCTTAA ACATCAGCTG CTCTGACATG GTGTTCAGCG GGCTCCTGCT GTACCTCTGT 
GACTCTTTTG TGGGGGCTAG CTTCTTGAAG AAGTTCCATT TTCTGAAAGG AGCCACCTTG 
TGTGTGATCT GCCAAGACCG GAACTCACTT CGCCAGACGG TGGTCCGCCT GGAGCTAGAA 
GATGAGTGGC AGTTCCGGCT GCGGGATGAG TTCCAGACAG CCAATGCCAA GGAAGACAGG 
CCGCTCTTTT TCCTCACCGC ACGGCACATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515100.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515163.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X8, mRNA.

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CloneID	OMu04813
Clone ID Related Accession (Same CDS sequence)	NM_001252071.1 , XM_006515100.2 , XM_006515100.3
Accession Version	XM_006515100.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAATTCCAG CACCCAGAAT 
CTGGACCTGG GGTCCTTCGA GAAGGTGGAC TTTCTCATTT GCATTCCACC GTCAGAGGTG 
ACCTACCAGC AGACCGTGTT CCACGTCTGG CATTCAGGGG TTCTGCTGGA GCTGGGCCTG 
GAGAAAGAGC CTGTGACCAA GCAGAGGGCG GAGCAACACG TTCTGAAACT AGACACGGAG 
GCCCAGGCAA GATTTAAGGC CTTTCTGCAA AACTCCTTCC AGAACCCACA TACGCTCTTT 
GTCCTCATCC ACGACCACGC CCACTGGGAT CTAGTGAGCA GTGCTGTTCA TAACATCTAC 
TCTCAAAGTG ACCCATCTGT GGGGTTAGTG GACAGACTGC TCAACTGCAG GGAGGTGAAA 
GAGGCGCCCA ACATTGTGAC CCTCCATGTG ACCTCTTTCC CGTATGCGCT GCAGACTCAG 
CACACCCTCA TTAGCCCATA CAATGAGATC CATTGGCCCA TCTCCTTCAG TAATGGAGTG 
GACCTGTATC ATGAGAGTAA GAAGTACTTC GGGCTGTCTG AGTTTATTGA CTCCACCCTG 
TCAGGACACA GTCTTCCTCT GCTCCGATAT GACAGTTCCT TTGAGGCCAT GGTCACTGCA 
TTGGGGAAAA GGTTCCCCCG CCTGCACAGC GCGGTCATCA GGACCTTTGT TCTTGTGCAG 
CACTATGCGG CCGCCATGAT GGCCGTGAGT GGCCTCCCGC AGATGAAGAA CCATACATCA 
GTGGAGACCC TAGAGATCAC CCAGAACCTC CTCAACTCCC CGAAGCAGTG CCCTTGTGGC 
CACGGGCTCA TGGTCCTGCT GCGCGTGCCC TGTTCACCCC TGGCAGCCGT GGCCTATGAG 
CGACTGGCCC ACGTGCGGGC TCGCTTGGCT CTGGAGGAAC ACTTTGAGAT CATCCTGGGC 
CACCCCAGCT CCGGTATCAC TGTAGGCAAG CACTTTGTGA AGCAGCTCAA GATGTGGCAG 
AAAATTGAGG ATGCAGAATG GAGGCCTCAG ACGTACTTAG AGTTGGAGGG CCTGCCCTGC 
ATCCTGATCT TCAGTGGGAT GGACCCTCAT GGGGAGTCAC TGCCCCGGTC TCTGAGGTAC 
TGTGACCTGC GTCTAATAAA CTCCTCCTGC CTGGTGAGGA CAGCCCTGGA GCAGGAGCTG 
GGTCTGGCTG CCTACTTTGT TAGCAATGAT ATCCCCTTGG AGAAGGGGCC CAAGAACGAG 
GCCTTGGAGA GTGATGGGGA GAAACTGAGC AGCACCGATG AAGACGAGGA GGCGGGGACA 
GAAGGGTGTT TTGAAGCAGG CTCTACGTCA GAGCAGAGGG GCCCTGTGAA GAGGGAACGC 
TCCCACTCCC ACGACTCTGC ATCGTCATCC CTCTCCTCCA GAGCATCTGG CTCGGTGCTC 
TATGGTGAGT CCCTGGCCCA GCCCTCAGGA CCTCCTCAGG GAGAGCTTAC CAGATCACCT 
CCATCCTGTG GCCCTGCAGA GGAAGGCAGG GCCCCTGGGG AAATCCAGCG CCTCCGAGTG 
AGTCAGGGGT CTACAGTCAT CAGTAGGCAC AGCCCTGGCC TGGTCCCACA GCCTGATTCT 
AGCCTCAGGA CTGGTCGGAG GAGCTTGCAG GTGCCAGCCG CCCCGTCCTC CCAGCTGTCC 
TCCTCCTCGG GCTCATCATC CACATGTGCG GTGCCCACTG CCAATGTGCT GGTCCTTCAA 
GCCTCTCAGT GCTCCATGGC CAAGGCCTGC CGGCAGCCAC CCATCGTCTT CCTGCCGAAG 
CTTGTGTACG ACATGCTCCT GTCCACCGAC AGCAGCGGCT TGCCCAAGTC TGCCTCCCTC 
CTGCCTTCCC CTTCAGTCAT GTGGACCAGC TCCTTCCGCC CCCTGCTCAG CAAGATGATG 
ACATCAACTG AGCAGTCTCT CTACTACCGG CAGTGGACAG TGCCCCGGCC CAGCCACATG 
GACTACGGCA ACAGAGCTGA GGGCCGTGTG GACAGCTTCC ACCCCCGCAG GCTGCTGCTC 
AGTGGGCCAC CTCAGATTGG GAAGACAGGT GCCTACCTGC AGTTCCTCAG TATCTTGTCC 
AGGATGCTGA TTCGGCTGAC AGAAGTGGAT GTTTACGACG AAGAAGAGAT CAATACCAGC 
TTCCGAGAGG AATCGGAGTG GCGTTACCTC CAGCTGGCCG ACCCCTGGCC AGACCTGGAG 
CTCTTCCAGA AGATGCCTTT TGATTACATC ATTCATGACC CGAAGTATGA GGACGCCAGT 
CTGATTTGTT CACACCCTCA GACCATCAAG AGTGAAGACA GAGGGATGTC CAGGAAGCCG 
GAGGACCTGT ATGTGCGGCG TCAGACGGCA CGTATGAGAC TGTCCAAGTA TGCAGCATAC 
AACACGTACC ACCACTGTGA GCAGTGCCAG CAGTACATGG GCTTCCACCC CCACTACCAG 
CTCTCTGAGT CCACCCTGCA CGTCTTTGCC TTCTCTTGCT CCATGCTAGG AGAGGAGGTC 
CAACTTCACT TCATCATCCC CAAGTCCAAG GAGCACCACT TTGTTTTCAG CCAACCCGGA 
GGCCAGCTGG AGAGCATGCG CTTGCCCCTC GTCACAGACA AGAGTCATGA ACACATCAAA 
AGCCCAACCT TCACACCGAC AACGGGCCGG CACGAGCACG GGCTCTTTAA TTTGTACCAT 
GCGATGGATG GAGCCAACCA CCTACACGTG CTGGTGGTCA AGGAGTACGA AATGGCCATT 
TATAAGAAAT ACTGGCCCAA CCACATCATG CTTGTCCTCC CAAGCATCTT TAACAGTGCT 
GGTGTCGGGG CTGCCCACTT CCTGATCAAA GAGCTTTGTT ACCACAACCT GGAGCTGGAG 
CGGAACCGGC AGGAGGAGCT GGGGGTCAAG CCTCAGGACG TCTGGCCCTT CATCGTCATT 
GCTGATGACT CCTGTGTGAT GTGGAATGTA GCAGATGTGG ACTGTGCCGG AGAACGGAGC 
AGGGAGTTTT CCTGGTCGGA GAGGAATGTC TCTCTGAAAT ACATCATGCT ACACATTGAG 
GCGTCCCCTA ACATCACACA CTATGCCCTG CTCGGCATGC GGAAGTGGGC CAGCAAGACC 
CGGGGCCGAG AGGTACAGGA GCCTTTTTCA CGATGTCATG TGCACGACTT CATCATCCTC 
AACGTGGACT TGACCCAGAA TGTACAGTAC AACCAGAACC GGTTTACATG TGACGATGTA 
GACTTCAACC TCCGTGTGCA CAGTGCTGGC CTCCTGCTCT GCCGGTTCAA TCGCTTCAGT 
GTCATGAAGA AGCAGATTGC AGTGGGTGGG CACAGGTCTT TCCACATCAC ATCCAAGGTA 
TCTGACAGCT CTGTGGCCAT TGTGCCGTCC CAGTACATCT GTGCCCCCGA CAGTAAGCAT 
ACGTTCCTGG CAGCTCCCGC CCAGCTCCTG CTGGAGAAAT TCCTCCAATA CCATAGCCAT 
CGCTTCTTCC CGCTGTCTCT GAAGAATCAC AGCCACCCCG TGTTGTCTGT AGACTGCTAC 
CTGAACCTGG GACCTCAGAT TTCTGTTTGT TATGTGAGCT CCAGGCCCCA CTCCTTAAAC 
ATCAGCTGCT CTGACATGGT GTTCAGCGGG CTCCTGCTGT ACCTCTGTGA CTCTTTTGTG 
GGGGCTAGCT TCTTGAAGAA GTTCCATTTT CTGAAAGGAG CCACCTTGTG TGTGATCTGC 
CAAGACCGGA ACTCACTTCG CCAGACGGTG GTCCGCCTGG AGCTAGAAGA TGAGTGGCAG 
TTCCGGCTGC GGGATGAGTT CCAGACAGCC AATGCCAAGG AAGACAGGCC GCTCTTTTTC 
CTCACCGCAC GGCACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515163.1
CDS	701..6481
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTNSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515100.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515100.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAATTCCAG CACCCAGAAT 
CTGGACCTGG GGTCCTTCGA GAAGGTGGAC TTTCTCATTT GCATTCCACC GTCAGAGGTG 
ACCTACCAGC AGACCGTGTT CCACGTCTGG CATTCAGGGG TTCTGCTGGA GCTGGGCCTG 
GAGAAAGAGC CTGTGACCAA GCAGAGGGCG GAGCAACACG TTCTGAAACT AGACACGGAG 
GCCCAGGCAA GATTTAAGGC CTTTCTGCAA AACTCCTTCC AGAACCCACA TACGCTCTTT 
GTCCTCATCC ACGACCACGC CCACTGGGAT CTAGTGAGCA GTGCTGTTCA TAACATCTAC 
TCTCAAAGTG ACCCATCTGT GGGGTTAGTG GACAGACTGC TCAACTGCAG GGAGGTGAAA 
GAGGCGCCCA ACATTGTGAC CCTCCATGTG ACCTCTTTCC CGTATGCGCT GCAGACTCAG 
CACACCCTCA TTAGCCCATA CAATGAGATC CATTGGCCCA TCTCCTTCAG TAATGGAGTG 
GACCTGTATC ATGAGAGTAA GAAGTACTTC GGGCTGTCTG AGTTTATTGA CTCCACCCTG 
TCAGGACACA GTCTTCCTCT GCTCCGATAT GACAGTTCCT TTGAGGCCAT GGTCACTGCA 
TTGGGGAAAA GGTTCCCCCG CCTGCACAGC GCGGTCATCA GGACCTTTGT TCTTGTGCAG 
CACTATGCGG CCGCCATGAT GGCCGTGAGT GGCCTCCCGC AGATGAAGAA CCATACATCA 
GTGGAGACCC TAGAGATCAC CCAGAACCTC CTCAACTCCC CGAAGCAGTG CCCTTGTGGC 
CACGGGCTCA TGGTCCTGCT GCGCGTGCCC TGTTCACCCC TGGCAGCCGT GGCCTATGAG 
CGACTGGCCC ACGTGCGGGC TCGCTTGGCT CTGGAGGAAC ACTTTGAGAT CATCCTGGGC 
CACCCCAGCT CCGGTATCAC TGTAGGCAAG CACTTTGTGA AGCAGCTCAA GATGTGGCAG 
AAAATTGAGG ATGCAGAATG GAGGCCTCAG ACGTACTTAG AGTTGGAGGG CCTGCCCTGC 
ATCCTGATCT TCAGTGGGAT GGACCCTCAT GGGGAGTCAC TGCCCCGGTC TCTGAGGTAC 
TGTGACCTGC GTCTAATAAA CTCCTCCTGC CTGGTGAGGA CAGCCCTGGA GCAGGAGCTG 
GGTCTGGCTG CCTACTTTGT TAGCAATGAT ATCCCCTTGG AGAAGGGGCC CAAGAACGAG 
GCCTTGGAGA GTGATGGGGA GAAACTGAGC AGCACCGATG AAGACGAGGA GGCGGGGACA 
GAAGGGTGTT TTGAAGCAGG CTCTACGTCA GAGCAGAGGG GCCCTGTGAA GAGGGAACGC 
TCCCACTCCC ACGACTCTGC ATCGTCATCC CTCTCCTCCA GAGCATCTGG CTCGGTGCTC 
TATGGTGAGT CCCTGGCCCA GCCCTCAGGA CCTCCTCAGG GAGAGCTTAC CAGATCACCT 
CCATCCTGTG GCCCTGCAGA GGAAGGCAGG GCCCCTGGGG AAATCCAGCG CCTCCGAGTG 
AGTCAGGGGT CTACAGTCAT CAGTAGGCAC AGCCCTGGCC TGGTCCCACA GCCTGATTCT 
AGCCTCAGGA CTGGTCGGAG GAGCTTGCAG GTGCCAGCCG CCCCGTCCTC CCAGCTGTCC 
TCCTCCTCGG GCTCATCATC CACATGTGCG GTGCCCACTG CCAATGTGCT GGTCCTTCAA 
GCCTCTCAGT GCTCCATGGC CAAGGCCTGC CGGCAGCCAC CCATCGTCTT CCTGCCGAAG 
CTTGTGTACG ACATGCTCCT GTCCACCGAC AGCAGCGGCT TGCCCAAGTC TGCCTCCCTC 
CTGCCTTCCC CTTCAGTCAT GTGGACCAGC TCCTTCCGCC CCCTGCTCAG CAAGATGATG 
ACATCAACTG AGCAGTCTCT CTACTACCGG CAGTGGACAG TGCCCCGGCC CAGCCACATG 
GACTACGGCA ACAGAGCTGA GGGCCGTGTG GACAGCTTCC ACCCCCGCAG GCTGCTGCTC 
AGTGGGCCAC CTCAGATTGG GAAGACAGGT GCCTACCTGC AGTTCCTCAG TATCTTGTCC 
AGGATGCTGA TTCGGCTGAC AGAAGTGGAT GTTTACGACG AAGAAGAGAT CAATACCAGC 
TTCCGAGAGG AATCGGAGTG GCGTTACCTC CAGCTGGCCG ACCCCTGGCC AGACCTGGAG 
CTCTTCCAGA AGATGCCTTT TGATTACATC ATTCATGACC CGAAGTATGA GGACGCCAGT 
CTGATTTGTT CACACCCTCA GACCATCAAG AGTGAAGACA GAGGGATGTC CAGGAAGCCG 
GAGGACCTGT ATGTGCGGCG TCAGACGGCA CGTATGAGAC TGTCCAAGTA TGCAGCATAC 
AACACGTACC ACCACTGTGA GCAGTGCCAG CAGTACATGG GCTTCCACCC CCACTACCAG 
CTCTCTGAGT CCACCCTGCA CGTCTTTGCC TTCTCTTGCT CCATGCTAGG AGAGGAGGTC 
CAACTTCACT TCATCATCCC CAAGTCCAAG GAGCACCACT TTGTTTTCAG CCAACCCGGA 
GGCCAGCTGG AGAGCATGCG CTTGCCCCTC GTCACAGACA AGAGTCATGA ACACATCAAA 
AGCCCAACCT TCACACCGAC AACGGGCCGG CACGAGCACG GGCTCTTTAA TTTGTACCAT 
GCGATGGATG GAGCCAACCA CCTACACGTG CTGGTGGTCA AGGAGTACGA AATGGCCATT 
TATAAGAAAT ACTGGCCCAA CCACATCATG CTTGTCCTCC CAAGCATCTT TAACAGTGCT 
GGTGTCGGGG CTGCCCACTT CCTGATCAAA GAGCTTTGTT ACCACAACCT GGAGCTGGAG 
CGGAACCGGC AGGAGGAGCT GGGGGTCAAG CCTCAGGACG TCTGGCCCTT CATCGTCATT 
GCTGATGACT CCTGTGTGAT GTGGAATGTA GCAGATGTGG ACTGTGCCGG AGAACGGAGC 
AGGGAGTTTT CCTGGTCGGA GAGGAATGTC TCTCTGAAAT ACATCATGCT ACACATTGAG 
GCGTCCCCTA ACATCACACA CTATGCCCTG CTCGGCATGC GGAAGTGGGC CAGCAAGACC 
CGGGGCCGAG AGGTACAGGA GCCTTTTTCA CGATGTCATG TGCACGACTT CATCATCCTC 
AACGTGGACT TGACCCAGAA TGTACAGTAC AACCAGAACC GGTTTACATG TGACGATGTA 
GACTTCAACC TCCGTGTGCA CAGTGCTGGC CTCCTGCTCT GCCGGTTCAA TCGCTTCAGT 
GTCATGAAGA AGCAGATTGC AGTGGGTGGG CACAGGTCTT TCCACATCAC ATCCAAGGTA 
TCTGACAGCT CTGTGGCCAT TGTGCCGTCC CAGTACATCT GTGCCCCCGA CAGTAAGCAT 
ACGTTCCTGG CAGCTCCCGC CCAGCTCCTG CTGGAGAAAT TCCTCCAATA CCATAGCCAT 
CGCTTCTTCC CGCTGTCTCT GAAGAATCAC AGCCACCCCG TGTTGTCTGT AGACTGCTAC 
CTGAACCTGG GACCTCAGAT TTCTGTTTGT TATGTGAGCT CCAGGCCCCA CTCCTTAAAC 
ATCAGCTGCT CTGACATGGT GTTCAGCGGG CTCCTGCTGT ACCTCTGTGA CTCTTTTGTG 
GGGGCTAGCT TCTTGAAGAA GTTCCATTTT CTGAAAGGAG CCACCTTGTG TGTGATCTGC 
CAAGACCGGA ACTCACTTCG CCAGACGGTG GTCCGCCTGG AGCTAGAAGA TGAGTGGCAG 
TTCCGGCTGC GGGATGAGTT CCAGACAGCC AATGCCAAGG AAGACAGGCC GCTCTTTTTC 
CTCACCGCAC GGCACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515093.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515156.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X1, mRNA.

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CloneID	OMu10233
Clone ID Related Accession (Same CDS sequence)	XM_006515093.3 , XM_006515094.3 , NM_015764.4 , XM_006515097.3 , XM_006515098.3 , XM_006515093.2 , XM_006515098.2 , XM_006515095.3 , XM_006515096.3 , XM_006515096.2
Accession Version	XM_006515093.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515093.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515156.1
CDS	701..6565
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515093.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515093.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515098.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515161.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X6, mRNA.

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CloneID	OMu10233
Clone ID Related Accession (Same CDS sequence)	XM_006515093.3 , XM_006515094.3 , NM_015764.4 , XM_006515097.3 , XM_006515098.3 , XM_006515093.2 , XM_006515098.2 , XM_006515095.3 , XM_006515096.3 , XM_006515096.2
Accession Version	XM_006515098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515161.1
CDS	492..6356
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515098.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515098.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515096.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515159.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X4, mRNA.

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CloneID	OMu10233
Clone ID Related Accession (Same CDS sequence)	XM_006515093.3 , XM_006515094.3 , NM_015764.4 , XM_006515097.3 , XM_006515098.3 , XM_006515093.2 , XM_006515098.2 , XM_006515095.3 , XM_006515096.3 , XM_006515096.2
Accession Version	XM_006515096.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515159.1
CDS	602..6466
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515096.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515096.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515099.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515162.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X7, mRNA.

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CloneID	OMu47321
Clone ID Related Accession (Same CDS sequence)	XM_006515099.2 , XM_006515099.3
Accession Version	XM_006515099.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515099.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGTGCTGTTC ATAACATCTA CTCTCAAAGT GACCCATCTG TGGGGTTAGT GGACAGACTG 
CTCAACTGCA GGGAGGTGAA AGAGGCGCCC AACATTGTGA CCCTCCATGT GACCTCTTTC 
CCGTATGCGC TGCAGACTCA GCACACCCTC ATTAGCCCAT ACAATGAGAT CCATTGGCCC 
ATCTCCTTCA GTAATGGAGT GGACCTGTAT CATGAGAGTA AGAAGTACTT CGGGCTGTCT 
GAGTTTATTG ACTCCACCCT GTCAGGACAC AGTCTTCCTC TGCTCCGATA TGACAGTTCC 
TTTGAGGCCA TGGTCACTGC ATTGGGGAAA AGGTTCCCCC GCCTGCACAG CGCGGTCATC 
AGGACCTTTG TTCTTGTGCA GCACTATGCG GCCGCCATGA TGGCCGTGAG TGGCCTCCCG 
CAGATGAAGA ACCATACATC AGTGGAGACC CTAGAGATCA CCCAGAACCT CCTCAACTCC 
CCGAAGCAGT GCCCTTGTGG CCACGGGCTC ATGGTCCTGC TGCGCGTGCC CTGTTCACCC 
CTGGCAGCCG TGGCCTATGA GCGACTGGCC CACGTGCGGG CTCGCTTGGC TCTGGAGGAA 
CACTTTGAGA TCATCCTGGG CCACCCCAGC TCCGGTATCA CTGTAGGCAA GCACTTTGTG 
AAGCAGCTCA AGATGTGGCA GAAAATTGAG GATGCAGAAT GGAGGCCTCA GACGTACTTA 
GAGTTGGAGG GCCTGCCCTG CATCCTGATC TTCAGTGGGA TGGACCCTCA TGGGGAGTCA 
CTGCCCCGGT CTCTGAGGTA CTGTGACCTG CGTCTAATAA ACTCCTCCTG CCTGGTGAGG 
ACAGCCCTGG AGCAGGAGCT GGGTCTGGCT GCCTACTTTG TTAGCAATGA TATCCCCTTG 
GAGAAGGGGC CCAAGAACGA GGCCTTGGAG AGTGATGGGG AGAAACTGAG CAGCACCGAT 
GAAGACGAGG AGGCGGGGAC AGAAGGGTGT TTTGAAGCAG GCTCTACGTC AGAGCAGAGG 
GGCCCTGTGA AGAGGGAACG CTCCCACTCC CACGACTCTG CATCGTCATC CCTCTCCTCC 
AGAGCATCTG GCTCGGTGCT CTATGGTGAG TCCCTGGCCC AGCCCTCAGG ACCTCCTCAG 
GGAGAGCTTA CCAGATCACC TCCATCCTGT GGCCCTGCAG AGGAAGGCAG GGCCCCTGGG 
GAAATCCAGC GCCTCCGAGT GAGTCAGGGG TCTACAGTCA TCAGTAGGCA CAGCCCTGGC 
CTGGTCCCAC AGCCTGATTC TAGCCTCAGG ACTGGTCGGA GGAGCTTGCA GGTGCCAGCC 
GCCCCGTCCT CCCAGCTGTC CTCCTCCTCG GGCTCATCAT CCACATGTGC GGTGCCCACT 
GCCAATGTGC TGGTCCTTCA AGCCTCTCAG TGCTCCATGG CCAAGGCCTG CCGGCAGCCA 
CCCATCGTCT TCCTGCCGAA GCTTGTGTAC GACATGCTCC TGTCCACCGA CAGCAGCGGC 
TTGCCCAAGT CTGCCTCCCT CCTGCCTTCC CCTTCAGTCA TGTGGACCAG CTCCTTCCGC 
CCCCTGCTCA GCAAGATGAT GACATCAACT GAGCAGTCTC TCTACTACCG GCAGTGGACA 
GTGCCCCGGC CCAGCCACAT GGACTACGGC AACAGAGCTG AGGGCCGTGT GGACAGCTTC 
CACCCCCGCA GGCTGCTGCT CAGTGGGCCA CCTCAGATTG GGAAGACAGG TGCCTACCTG 
CAGTTCCTCA GTATCTTGTC CAGGATGCTG ATTCGGCTGA CAGAAGTGGA TGTTTACGAC 
GAAGAAGAGA TCAATACCAG CTTCCGAGAG GAATCGGAGT GGCGTTACCT CCAGCTGGCC 
GACCCCTGGC CAGACCTGGA GCTCTTCCAG AAGATGCCTT TTGATTACAT CATTCATGAC 
CCGAAGTATG AGGACGCCAG TCTGATTTGT TCACACCCTC AGACCATCAA GAGTGAAGAC 
AGAGGGATGT CCAGGAAGCC GGAGGACCTG TATGTGCGGC GTCAGACGGC ACGTATGAGA 
CTGTCCAAGT ATGCAGCATA CAACACGTAC CACCACTGTG AGCAGTGCCA GCAGTACATG 
GGCTTCCACC CCCACTACCA GCTCTCTGAG TCCACCCTGC ACGTCTTTGC CTTCTCTTGC 
TCCATGCTAG GAGAGGAGGT CCAACTTCAC TTCATCATCC CCAAGTCCAA GGAGCACCAC 
TTTGTTTTCA GCCAACCCGG AGGCCAGCTG GAGAGCATGC GCTTGCCCCT CGTCACAGAC 
AAGAGTCATG AACACATCAA AAGCCCAACC TTCACACCGA CAACGGGCCG GCACGAGCAC 
GGGCTCTTTA ATTTGTACCA TGCGATGGAT GGAGCCAACC ACCTACACGT GCTGGTGGTC 
AAGGAGTACG AAATGGCCAT TTATAAGAAA TACTGGCCCA ACCACATCAT GCTTGTCCTC 
CCAAGCATCT TTAACAGTGC TGGTGTCGGG GCTGCCCACT TCCTGATCAA AGAGCTTTGT 
TACCACAACC TGGAGCTGGA GCGGAACCGG CAGGAGGAGC TGGGGGTCAA GCCTCAGGAC 
GTCTGGCCCT TCATCGTCAT TGCTGATGAC TCCTGTGTGA TGTGGAATGT AGCAGATGTG 
GACTGTGCCG GAGAACGGAG CAGGGAGTTT TCCTGGTCGG AGAGGAATGT CTCTCTGAAA 
TACATCATGC TACACATTGA GGCGTCCCCT AACATCACAC ACTATGCCCT GCTCGGCATG 
CGGAAGTGGG CCAGCAAGAC CCGGGGCCGA GAGGTACAGG AGCCTTTTTC ACGATGTCAT 
GTGCACGACT TCATCATCCT CAACGTGGAC TTGACCCAGA ATGTACAGTA CAACCAGAAC 
CGGTTTACAT GTGACGATGT AGACTTCAAC CTCCGTGTGC ACAGTGCTGG CCTCCTGCTC 
TGCCGGTTCA ATCGCTTCAG TGTCATGAAG AAGCAGATTG CAGTGGGTGG GCACAGGTCT 
TTCCACATCA CATCCAAGGT ATCTGACAGC TCTGTGGCCA TTGTGCCGTC CCAGTACATC 
TGTGCCCCCG ACAGTAAGCA TACGTTCCTG GCAGCTCCCG CCCAGCTCCT GCTGGAGAAA 
TTCCTCCAAT ACCATAGCCA TCGCTTCTTC CCGCTGTCTC TGAAGAATCA CAGCCACCCC 
GTGTTGTCTG TAGACTGCTA CCTGAACCTG GGACCTCAGA TTTCTGTTTG TTATGTGAGC 
TCCAGGCCCC ACTCCTTAAA CATCAGCTGC TCTGACATGG TGTTCAGCGG GCTCCTGCTG 
TACCTCTGTG ACTCTTTTGT GGGGGCTAGC TTCTTGAAGA AGTTCCATTT TCTGAAAGGA 
GCCACCTTGT GTGTGATCTG CCAAGACCGG AACTCACTTC GCCAGACGGT GGTCCGCCTG 
GAGCTAGAAG ATGAGTGGCA GTTCCGGCTG CGGGATGAGT TCCAGACAGC CAATGCCAAG 
GAAGACAGGC CGCTCTTTTT CCTCACCGCA CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515162.1
CDS	701..6562
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515099.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515099.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGTGCTGTTC ATAACATCTA CTCTCAAAGT GACCCATCTG TGGGGTTAGT GGACAGACTG 
CTCAACTGCA GGGAGGTGAA AGAGGCGCCC AACATTGTGA CCCTCCATGT GACCTCTTTC 
CCGTATGCGC TGCAGACTCA GCACACCCTC ATTAGCCCAT ACAATGAGAT CCATTGGCCC 
ATCTCCTTCA GTAATGGAGT GGACCTGTAT CATGAGAGTA AGAAGTACTT CGGGCTGTCT 
GAGTTTATTG ACTCCACCCT GTCAGGACAC AGTCTTCCTC TGCTCCGATA TGACAGTTCC 
TTTGAGGCCA TGGTCACTGC ATTGGGGAAA AGGTTCCCCC GCCTGCACAG CGCGGTCATC 
AGGACCTTTG TTCTTGTGCA GCACTATGCG GCCGCCATGA TGGCCGTGAG TGGCCTCCCG 
CAGATGAAGA ACCATACATC AGTGGAGACC CTAGAGATCA CCCAGAACCT CCTCAACTCC 
CCGAAGCAGT GCCCTTGTGG CCACGGGCTC ATGGTCCTGC TGCGCGTGCC CTGTTCACCC 
CTGGCAGCCG TGGCCTATGA GCGACTGGCC CACGTGCGGG CTCGCTTGGC TCTGGAGGAA 
CACTTTGAGA TCATCCTGGG CCACCCCAGC TCCGGTATCA CTGTAGGCAA GCACTTTGTG 
AAGCAGCTCA AGATGTGGCA GAAAATTGAG GATGCAGAAT GGAGGCCTCA GACGTACTTA 
GAGTTGGAGG GCCTGCCCTG CATCCTGATC TTCAGTGGGA TGGACCCTCA TGGGGAGTCA 
CTGCCCCGGT CTCTGAGGTA CTGTGACCTG CGTCTAATAA ACTCCTCCTG CCTGGTGAGG 
ACAGCCCTGG AGCAGGAGCT GGGTCTGGCT GCCTACTTTG TTAGCAATGA TATCCCCTTG 
GAGAAGGGGC CCAAGAACGA GGCCTTGGAG AGTGATGGGG AGAAACTGAG CAGCACCGAT 
GAAGACGAGG AGGCGGGGAC AGAAGGGTGT TTTGAAGCAG GCTCTACGTC AGAGCAGAGG 
GGCCCTGTGA AGAGGGAACG CTCCCACTCC CACGACTCTG CATCGTCATC CCTCTCCTCC 
AGAGCATCTG GCTCGGTGCT CTATGGTGAG TCCCTGGCCC AGCCCTCAGG ACCTCCTCAG 
GGAGAGCTTA CCAGATCACC TCCATCCTGT GGCCCTGCAG AGGAAGGCAG GGCCCCTGGG 
GAAATCCAGC GCCTCCGAGT GAGTCAGGGG TCTACAGTCA TCAGTAGGCA CAGCCCTGGC 
CTGGTCCCAC AGCCTGATTC TAGCCTCAGG ACTGGTCGGA GGAGCTTGCA GGTGCCAGCC 
GCCCCGTCCT CCCAGCTGTC CTCCTCCTCG GGCTCATCAT CCACATGTGC GGTGCCCACT 
GCCAATGTGC TGGTCCTTCA AGCCTCTCAG TGCTCCATGG CCAAGGCCTG CCGGCAGCCA 
CCCATCGTCT TCCTGCCGAA GCTTGTGTAC GACATGCTCC TGTCCACCGA CAGCAGCGGC 
TTGCCCAAGT CTGCCTCCCT CCTGCCTTCC CCTTCAGTCA TGTGGACCAG CTCCTTCCGC 
CCCCTGCTCA GCAAGATGAT GACATCAACT GAGCAGTCTC TCTACTACCG GCAGTGGACA 
GTGCCCCGGC CCAGCCACAT GGACTACGGC AACAGAGCTG AGGGCCGTGT GGACAGCTTC 
CACCCCCGCA GGCTGCTGCT CAGTGGGCCA CCTCAGATTG GGAAGACAGG TGCCTACCTG 
CAGTTCCTCA GTATCTTGTC CAGGATGCTG ATTCGGCTGA CAGAAGTGGA TGTTTACGAC 
GAAGAAGAGA TCAATACCAG CTTCCGAGAG GAATCGGAGT GGCGTTACCT CCAGCTGGCC 
GACCCCTGGC CAGACCTGGA GCTCTTCCAG AAGATGCCTT TTGATTACAT CATTCATGAC 
CCGAAGTATG AGGACGCCAG TCTGATTTGT TCACACCCTC AGACCATCAA GAGTGAAGAC 
AGAGGGATGT CCAGGAAGCC GGAGGACCTG TATGTGCGGC GTCAGACGGC ACGTATGAGA 
CTGTCCAAGT ATGCAGCATA CAACACGTAC CACCACTGTG AGCAGTGCCA GCAGTACATG 
GGCTTCCACC CCCACTACCA GCTCTCTGAG TCCACCCTGC ACGTCTTTGC CTTCTCTTGC 
TCCATGCTAG GAGAGGAGGT CCAACTTCAC TTCATCATCC CCAAGTCCAA GGAGCACCAC 
TTTGTTTTCA GCCAACCCGG AGGCCAGCTG GAGAGCATGC GCTTGCCCCT CGTCACAGAC 
AAGAGTCATG AACACATCAA AAGCCCAACC TTCACACCGA CAACGGGCCG GCACGAGCAC 
GGGCTCTTTA ATTTGTACCA TGCGATGGAT GGAGCCAACC ACCTACACGT GCTGGTGGTC 
AAGGAGTACG AAATGGCCAT TTATAAGAAA TACTGGCCCA ACCACATCAT GCTTGTCCTC 
CCAAGCATCT TTAACAGTGC TGGTGTCGGG GCTGCCCACT TCCTGATCAA AGAGCTTTGT 
TACCACAACC TGGAGCTGGA GCGGAACCGG CAGGAGGAGC TGGGGGTCAA GCCTCAGGAC 
GTCTGGCCCT TCATCGTCAT TGCTGATGAC TCCTGTGTGA TGTGGAATGT AGCAGATGTG 
GACTGTGCCG GAGAACGGAG CAGGGAGTTT TCCTGGTCGG AGAGGAATGT CTCTCTGAAA 
TACATCATGC TACACATTGA GGCGTCCCCT AACATCACAC ACTATGCCCT GCTCGGCATG 
CGGAAGTGGG CCAGCAAGAC CCGGGGCCGA GAGGTACAGG AGCCTTTTTC ACGATGTCAT 
GTGCACGACT TCATCATCCT CAACGTGGAC TTGACCCAGA ATGTACAGTA CAACCAGAAC 
CGGTTTACAT GTGACGATGT AGACTTCAAC CTCCGTGTGC ACAGTGCTGG CCTCCTGCTC 
TGCCGGTTCA ATCGCTTCAG TGTCATGAAG AAGCAGATTG CAGTGGGTGG GCACAGGTCT 
TTCCACATCA CATCCAAGGT ATCTGACAGC TCTGTGGCCA TTGTGCCGTC CCAGTACATC 
TGTGCCCCCG ACAGTAAGCA TACGTTCCTG GCAGCTCCCG CCCAGCTCCT GCTGGAGAAA 
TTCCTCCAAT ACCATAGCCA TCGCTTCTTC CCGCTGTCTC TGAAGAATCA CAGCCACCCC 
GTGTTGTCTG TAGACTGCTA CCTGAACCTG GGACCTCAGA TTTCTGTTTG TTATGTGAGC 
TCCAGGCCCC ACTCCTTAAA CATCAGCTGC TCTGACATGG TGTTCAGCGG GCTCCTGCTG 
TACCTCTGTG ACTCTTTTGT GGGGGCTAGC TTCTTGAAGA AGTTCCATTT TCTGAAAGGA 
GCCACCTTGT GTGTGATCTG CCAAGACCGG AACTCACTTC GCCAGACGGT GGTCCGCCTG 
GAGCTAGAAG ATGAGTGGCA GTTCCGGCTG CGGGATGAGT TCCAGACAGC CAATGCCAAG 
GAAGACAGGC CGCTCTTTTT CCTCACCGCA CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515101.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515164.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X9, mRNA.

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CloneID	OMu47322
Clone ID Related Accession (Same CDS sequence)	XM_006515101.4 , XM_006515101.3
Accession Version	XM_006515101.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515101.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGGAGCCACC TTGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515164.1
CDS	701..6280
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQEPPCV

Target ORF information:

RefSeq Version	XM_006515101.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515101.4

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGGAGCCACC TTGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( NM_001252071.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239000.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant 2, mRNA.

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CloneID	OMu04813
Clone ID Related Accession (Same CDS sequence)	NM_001252071.1 , XM_006515100.2 , XM_006515100.3
Accession Version	NM_001252071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122228.4 and CN536594.1. Transcript Variant: This variant (2) differs in the 5' UTR, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC160931.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAATTCCAG CACCCAGAAT 
CTGGACCTGG GGTCCTTCGA GAAGGTGGAC TTTCTCATTT GCATTCCACC GTCAGAGGTG 
ACCTACCAGC AGACCGTGTT CCACGTCTGG CATTCAGGGG TTCTGCTGGA GCTGGGCCTG 
GAGAAAGAGC CTGTGACCAA GCAGAGGGCG GAGCAACACG TTCTGAAACT AGACACGGAG 
GCCCAGGCAA GATTTAAGGC CTTTCTGCAA AACTCCTTCC AGAACCCACA TACGCTCTTT 
GTCCTCATCC ACGACCACGC CCACTGGGAT CTAGTGAGCA GTGCTGTTCA TAACATCTAC 
TCTCAAAGTG ACCCATCTGT GGGGTTAGTG GACAGACTGC TCAACTGCAG GGAGGTGAAA 
GAGGCGCCCA ACATTGTGAC CCTCCATGTG ACCTCTTTCC CGTATGCGCT GCAGACTCAG 
CACACCCTCA TTAGCCCATA CAATGAGATC CATTGGCCCA TCTCCTTCAG TAATGGAGTG 
GACCTGTATC ATGAGAGTAA GAAGTACTTC GGGCTGTCTG AGTTTATTGA CTCCACCCTG 
TCAGGACACA GTCTTCCTCT GCTCCGATAT GACAGTTCCT TTGAGGCCAT GGTCACTGCA 
TTGGGGAAAA GGTTCCCCCG CCTGCACAGC GCGGTCATCA GGACCTTTGT TCTTGTGCAG 
CACTATGCGG CCGCCATGAT GGCCGTGAGT GGCCTCCCGC AGATGAAGAA CCATACATCA 
GTGGAGACCC TAGAGATCAC CCAGAACCTC CTCAACTCCC CGAAGCAGTG CCCTTGTGGC 
CACGGGCTCA TGGTCCTGCT GCGCGTGCCC TGTTCACCCC TGGCAGCCGT GGCCTATGAG 
CGACTGGCCC ACGTGCGGGC TCGCTTGGCT CTGGAGGAAC ACTTTGAGAT CATCCTGGGC 
CACCCCAGCT CCGGTATCAC TGTAGGCAAG CACTTTGTGA AGCAGCTCAA GATGTGGCAG 
AAAATTGAGG ATGCAGAATG GAGGCCTCAG ACGTACTTAG AGTTGGAGGG CCTGCCCTGC 
ATCCTGATCT TCAGTGGGAT GGACCCTCAT GGGGAGTCAC TGCCCCGGTC TCTGAGGTAC 
TGTGACCTGC GTCTAATAAA CTCCTCCTGC CTGGTGAGGA CAGCCCTGGA GCAGGAGCTG 
GGTCTGGCTG CCTACTTTGT TAGCAATGAT ATCCCCTTGG AGAAGGGGCC CAAGAACGAG 
GCCTTGGAGA GTGATGGGGA GAAACTGAGC AGCACCGATG AAGACGAGGA GGCGGGGACA 
GAAGGGTGTT TTGAAGCAGG CTCTACGTCA GAGCAGAGGG GCCCTGTGAA GAGGGAACGC 
TCCCACTCCC ACGACTCTGC ATCGTCATCC CTCTCCTCCA GAGCATCTGG CTCGGTGCTC 
TATGGTGAGT CCCTGGCCCA GCCCTCAGGA CCTCCTCAGG GAGAGCTTAC CAGATCACCT 
CCATCCTGTG GCCCTGCAGA GGAAGGCAGG GCCCCTGGGG AAATCCAGCG CCTCCGAGTG 
AGTCAGGGGT CTACAGTCAT CAGTAGGCAC AGCCCTGGCC TGGTCCCACA GCCTGATTCT 
AGCCTCAGGA CTGGTCGGAG GAGCTTGCAG GTGCCAGCCG CCCCGTCCTC CCAGCTGTCC 
TCCTCCTCGG GCTCATCATC CACATGTGCG GTGCCCACTG CCAATGTGCT GGTCCTTCAA 
GCCTCTCAGT GCTCCATGGC CAAGGCCTGC CGGCAGCCAC CCATCGTCTT CCTGCCGAAG 
CTTGTGTACG ACATGCTCCT GTCCACCGAC AGCAGCGGCT TGCCCAAGTC TGCCTCCCTC 
CTGCCTTCCC CTTCAGTCAT GTGGACCAGC TCCTTCCGCC CCCTGCTCAG CAAGATGATG 
ACATCAACTG AGCAGTCTCT CTACTACCGG CAGTGGACAG TGCCCCGGCC CAGCCACATG 
GACTACGGCA ACAGAGCTGA GGGCCGTGTG GACAGCTTCC ACCCCCGCAG GCTGCTGCTC 
AGTGGGCCAC CTCAGATTGG GAAGACAGGT GCCTACCTGC AGTTCCTCAG TATCTTGTCC 
AGGATGCTGA TTCGGCTGAC AGAAGTGGAT GTTTACGACG AAGAAGAGAT CAATACCAGC 
TTCCGAGAGG AATCGGAGTG GCGTTACCTC CAGCTGGCCG ACCCCTGGCC AGACCTGGAG 
CTCTTCCAGA AGATGCCTTT TGATTACATC ATTCATGACC CGAAGTATGA GGACGCCAGT 
CTGATTTGTT CACACCCTCA GACCATCAAG AGTGAAGACA GAGGGATGTC CAGGAAGCCG 
GAGGACCTGT ATGTGCGGCG TCAGACGGCA CGTATGAGAC TGTCCAAGTA TGCAGCATAC 
AACACGTACC ACCACTGTGA GCAGTGCCAG CAGTACATGG GCTTCCACCC CCACTACCAG 
CTCTCTGAGT CCACCCTGCA CGTCTTTGCC TTCTCTTGCT CCATGCTAGG AGAGGAGGTC 
CAACTTCACT TCATCATCCC CAAGTCCAAG GAGCACCACT TTGTTTTCAG CCAACCCGGA 
GGCCAGCTGG AGAGCATGCG CTTGCCCCTC GTCACAGACA AGAGTCATGA ACACATCAAA 
AGCCCAACCT TCACACCGAC AACGGGCCGG CACGAGCACG GGCTCTTTAA TTTGTACCAT 
GCGATGGATG GAGCCAACCA CCTACACGTG CTGGTGGTCA AGGAGTACGA AATGGCCATT 
TATAAGAAAT ACTGGCCCAA CCACATCATG CTTGTCCTCC CAAGCATCTT TAACAGTGCT 
GGTGTCGGGG CTGCCCACTT CCTGATCAAA GAGCTTTGTT ACCACAACCT GGAGCTGGAG 
CGGAACCGGC AGGAGGAGCT GGGGGTCAAG CCTCAGGACG TCTGGCCCTT CATCGTCATT 
GCTGATGACT CCTGTGTGAT GTGGAATGTA GCAGATGTGG ACTGTGCCGG AGAACGGAGC 
AGGGAGTTTT CCTGGTCGGA GAGGAATGTC TCTCTGAAAT ACATCATGCT ACACATTGAG 
GCGTCCCCTA ACATCACACA CTATGCCCTG CTCGGCATGC GGAAGTGGGC CAGCAAGACC 
CGGGGCCGAG AGGTACAGGA GCCTTTTTCA CGATGTCATG TGCACGACTT CATCATCCTC 
AACGTGGACT TGACCCAGAA TGTACAGTAC AACCAGAACC GGTTTACATG TGACGATGTA 
GACTTCAACC TCCGTGTGCA CAGTGCTGGC CTCCTGCTCT GCCGGTTCAA TCGCTTCAGT 
GTCATGAAGA AGCAGATTGC AGTGGGTGGG CACAGGTCTT TCCACATCAC ATCCAAGGTA 
TCTGACAGCT CTGTGGCCAT TGTGCCGTCC CAGTACATCT GTGCCCCCGA CAGTAAGCAT 
ACGTTCCTGG CAGCTCCCGC CCAGCTCCTG CTGGAGAAAT TCCTCCAATA CCATAGCCAT 
CGCTTCTTCC CGCTGTCTCT GAAGAATCAC AGCCACCCCG TGTTGTCTGT AGACTGCTAC 
CTGAACCTGG GACCTCAGAT TTCTGTTTGT TATGTGAGCT CCAGGCCCCA CTCCTTAAAC 
ATCAGCTGCT CTGACATGGT GTTCAGCGGG CTCCTGCTGT ACCTCTGTGA CTCTTTTGTG 
GGGGCTAGCT TCTTGAAGAA GTTCCATTTT CTGAAAGGAG CCACCTTGTG TGTGATCTGC 
CAAGACCGGA ACTCACTTCG CCAGACGGTG GTCCGCCTGG AGCTAGAAGA TGAGTGGCAG 
TTCCGGCTGC GGGATGAGTT CCAGACAGCC AATGCCAAGG AAGACAGGCC GCTCTTTTTC 
CTCACCGCAC GGCACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239000.1
CDS	309..6089
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTNSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	NM_001252071.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252071.1

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAATTCCAG CACCCAGAAT 
CTGGACCTGG GGTCCTTCGA GAAGGTGGAC TTTCTCATTT GCATTCCACC GTCAGAGGTG 
ACCTACCAGC AGACCGTGTT CCACGTCTGG CATTCAGGGG TTCTGCTGGA GCTGGGCCTG 
GAGAAAGAGC CTGTGACCAA GCAGAGGGCG GAGCAACACG TTCTGAAACT AGACACGGAG 
GCCCAGGCAA GATTTAAGGC CTTTCTGCAA AACTCCTTCC AGAACCCACA TACGCTCTTT 
GTCCTCATCC ACGACCACGC CCACTGGGAT CTAGTGAGCA GTGCTGTTCA TAACATCTAC 
TCTCAAAGTG ACCCATCTGT GGGGTTAGTG GACAGACTGC TCAACTGCAG GGAGGTGAAA 
GAGGCGCCCA ACATTGTGAC CCTCCATGTG ACCTCTTTCC CGTATGCGCT GCAGACTCAG 
CACACCCTCA TTAGCCCATA CAATGAGATC CATTGGCCCA TCTCCTTCAG TAATGGAGTG 
GACCTGTATC ATGAGAGTAA GAAGTACTTC GGGCTGTCTG AGTTTATTGA CTCCACCCTG 
TCAGGACACA GTCTTCCTCT GCTCCGATAT GACAGTTCCT TTGAGGCCAT GGTCACTGCA 
TTGGGGAAAA GGTTCCCCCG CCTGCACAGC GCGGTCATCA GGACCTTTGT TCTTGTGCAG 
CACTATGCGG CCGCCATGAT GGCCGTGAGT GGCCTCCCGC AGATGAAGAA CCATACATCA 
GTGGAGACCC TAGAGATCAC CCAGAACCTC CTCAACTCCC CGAAGCAGTG CCCTTGTGGC 
CACGGGCTCA TGGTCCTGCT GCGCGTGCCC TGTTCACCCC TGGCAGCCGT GGCCTATGAG 
CGACTGGCCC ACGTGCGGGC TCGCTTGGCT CTGGAGGAAC ACTTTGAGAT CATCCTGGGC 
CACCCCAGCT CCGGTATCAC TGTAGGCAAG CACTTTGTGA AGCAGCTCAA GATGTGGCAG 
AAAATTGAGG ATGCAGAATG GAGGCCTCAG ACGTACTTAG AGTTGGAGGG CCTGCCCTGC 
ATCCTGATCT TCAGTGGGAT GGACCCTCAT GGGGAGTCAC TGCCCCGGTC TCTGAGGTAC 
TGTGACCTGC GTCTAATAAA CTCCTCCTGC CTGGTGAGGA CAGCCCTGGA GCAGGAGCTG 
GGTCTGGCTG CCTACTTTGT TAGCAATGAT ATCCCCTTGG AGAAGGGGCC CAAGAACGAG 
GCCTTGGAGA GTGATGGGGA GAAACTGAGC AGCACCGATG AAGACGAGGA GGCGGGGACA 
GAAGGGTGTT TTGAAGCAGG CTCTACGTCA GAGCAGAGGG GCCCTGTGAA GAGGGAACGC 
TCCCACTCCC ACGACTCTGC ATCGTCATCC CTCTCCTCCA GAGCATCTGG CTCGGTGCTC 
TATGGTGAGT CCCTGGCCCA GCCCTCAGGA CCTCCTCAGG GAGAGCTTAC CAGATCACCT 
CCATCCTGTG GCCCTGCAGA GGAAGGCAGG GCCCCTGGGG AAATCCAGCG CCTCCGAGTG 
AGTCAGGGGT CTACAGTCAT CAGTAGGCAC AGCCCTGGCC TGGTCCCACA GCCTGATTCT 
AGCCTCAGGA CTGGTCGGAG GAGCTTGCAG GTGCCAGCCG CCCCGTCCTC CCAGCTGTCC 
TCCTCCTCGG GCTCATCATC CACATGTGCG GTGCCCACTG CCAATGTGCT GGTCCTTCAA 
GCCTCTCAGT GCTCCATGGC CAAGGCCTGC CGGCAGCCAC CCATCGTCTT CCTGCCGAAG 
CTTGTGTACG ACATGCTCCT GTCCACCGAC AGCAGCGGCT TGCCCAAGTC TGCCTCCCTC 
CTGCCTTCCC CTTCAGTCAT GTGGACCAGC TCCTTCCGCC CCCTGCTCAG CAAGATGATG 
ACATCAACTG AGCAGTCTCT CTACTACCGG CAGTGGACAG TGCCCCGGCC CAGCCACATG 
GACTACGGCA ACAGAGCTGA GGGCCGTGTG GACAGCTTCC ACCCCCGCAG GCTGCTGCTC 
AGTGGGCCAC CTCAGATTGG GAAGACAGGT GCCTACCTGC AGTTCCTCAG TATCTTGTCC 
AGGATGCTGA TTCGGCTGAC AGAAGTGGAT GTTTACGACG AAGAAGAGAT CAATACCAGC 
TTCCGAGAGG AATCGGAGTG GCGTTACCTC CAGCTGGCCG ACCCCTGGCC AGACCTGGAG 
CTCTTCCAGA AGATGCCTTT TGATTACATC ATTCATGACC CGAAGTATGA GGACGCCAGT 
CTGATTTGTT CACACCCTCA GACCATCAAG AGTGAAGACA GAGGGATGTC CAGGAAGCCG 
GAGGACCTGT ATGTGCGGCG TCAGACGGCA CGTATGAGAC TGTCCAAGTA TGCAGCATAC 
AACACGTACC ACCACTGTGA GCAGTGCCAG CAGTACATGG GCTTCCACCC CCACTACCAG 
CTCTCTGAGT CCACCCTGCA CGTCTTTGCC TTCTCTTGCT CCATGCTAGG AGAGGAGGTC 
CAACTTCACT TCATCATCCC CAAGTCCAAG GAGCACCACT TTGTTTTCAG CCAACCCGGA 
GGCCAGCTGG AGAGCATGCG CTTGCCCCTC GTCACAGACA AGAGTCATGA ACACATCAAA 
AGCCCAACCT TCACACCGAC AACGGGCCGG CACGAGCACG GGCTCTTTAA TTTGTACCAT 
GCGATGGATG GAGCCAACCA CCTACACGTG CTGGTGGTCA AGGAGTACGA AATGGCCATT 
TATAAGAAAT ACTGGCCCAA CCACATCATG CTTGTCCTCC CAAGCATCTT TAACAGTGCT 
GGTGTCGGGG CTGCCCACTT CCTGATCAAA GAGCTTTGTT ACCACAACCT GGAGCTGGAG 
CGGAACCGGC AGGAGGAGCT GGGGGTCAAG CCTCAGGACG TCTGGCCCTT CATCGTCATT 
GCTGATGACT CCTGTGTGAT GTGGAATGTA GCAGATGTGG ACTGTGCCGG AGAACGGAGC 
AGGGAGTTTT CCTGGTCGGA GAGGAATGTC TCTCTGAAAT ACATCATGCT ACACATTGAG 
GCGTCCCCTA ACATCACACA CTATGCCCTG CTCGGCATGC GGAAGTGGGC CAGCAAGACC 
CGGGGCCGAG AGGTACAGGA GCCTTTTTCA CGATGTCATG TGCACGACTT CATCATCCTC 
AACGTGGACT TGACCCAGAA TGTACAGTAC AACCAGAACC GGTTTACATG TGACGATGTA 
GACTTCAACC TCCGTGTGCA CAGTGCTGGC CTCCTGCTCT GCCGGTTCAA TCGCTTCAGT 
GTCATGAAGA AGCAGATTGC AGTGGGTGGG CACAGGTCTT TCCACATCAC ATCCAAGGTA 
TCTGACAGCT CTGTGGCCAT TGTGCCGTCC CAGTACATCT GTGCCCCCGA CAGTAAGCAT 
ACGTTCCTGG CAGCTCCCGC CCAGCTCCTG CTGGAGAAAT TCCTCCAATA CCATAGCCAT 
CGCTTCTTCC CGCTGTCTCT GAAGAATCAC AGCCACCCCG TGTTGTCTGT AGACTGCTAC 
CTGAACCTGG GACCTCAGAT TTCTGTTTGT TATGTGAGCT CCAGGCCCCA CTCCTTAAAC 
ATCAGCTGCT CTGACATGGT GTTCAGCGGG CTCCTGCTGT ACCTCTGTGA CTCTTTTGTG 
GGGGCTAGCT TCTTGAAGAA GTTCCATTTT CTGAAAGGAG CCACCTTGTG TGTGATCTGC 
CAAGACCGGA ACTCACTTCG CCAGACGGTG GTCCGCCTGG AGCTAGAAGA TGAGTGGCAG 
TTCCGGCTGC GGGATGAGTT CCAGACAGCC AATGCCAAGG AAGACAGGCC GCTCTTTTTC 
CTCACCGCAC GGCACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( NM_015764.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_056579.2 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant 1, mRNA.

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CloneID	OMu10233
Clone ID Related Accession (Same CDS sequence)	XM_006515093.3 , XM_006515094.3 , NM_015764.4 , XM_006515097.3 , XM_006515098.3 , XM_006515093.2 , XM_006515098.2 , XM_006515095.3 , XM_006515096.3 , XM_006515096.2
Accession Version	NM_015764.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ098754.1, AC157352.2, CJ099922.1, AC122228.4 and CN536594.1. On Nov 3, 2011 this sequence version replaced NM_015764.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF180470.1, AK147337.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056579.2
CDS	631..6495
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	NM_015764.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015764.4

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515100.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515163.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X8, mRNA.

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CloneID	OMu04813
Clone ID Related Accession (Same CDS sequence)	NM_001252071.1 , XM_006515100.2 , XM_006515100.3
Accession Version	XM_006515100.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006515100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAATTCCAG CACCCAGAAT 
CTGGACCTGG GGTCCTTCGA GAAGGTGGAC TTTCTCATTT GCATTCCACC GTCAGAGGTG 
ACCTACCAGC AGACCGTGTT CCACGTCTGG CATTCAGGGG TTCTGCTGGA GCTGGGCCTG 
GAGAAAGAGC CTGTGACCAA GCAGAGGGCG GAGCAACACG TTCTGAAACT AGACACGGAG 
GCCCAGGCAA GATTTAAGGC CTTTCTGCAA AACTCCTTCC AGAACCCACA TACGCTCTTT 
GTCCTCATCC ACGACCACGC CCACTGGGAT CTAGTGAGCA GTGCTGTTCA TAACATCTAC 
TCTCAAAGTG ACCCATCTGT GGGGTTAGTG GACAGACTGC TCAACTGCAG GGAGGTGAAA 
GAGGCGCCCA ACATTGTGAC CCTCCATGTG ACCTCTTTCC CGTATGCGCT GCAGACTCAG 
CACACCCTCA TTAGCCCATA CAATGAGATC CATTGGCCCA TCTCCTTCAG TAATGGAGTG 
GACCTGTATC ATGAGAGTAA GAAGTACTTC GGGCTGTCTG AGTTTATTGA CTCCACCCTG 
TCAGGACACA GTCTTCCTCT GCTCCGATAT GACAGTTCCT TTGAGGCCAT GGTCACTGCA 
TTGGGGAAAA GGTTCCCCCG CCTGCACAGC GCGGTCATCA GGACCTTTGT TCTTGTGCAG 
CACTATGCGG CCGCCATGAT GGCCGTGAGT GGCCTCCCGC AGATGAAGAA CCATACATCA 
GTGGAGACCC TAGAGATCAC CCAGAACCTC CTCAACTCCC CGAAGCAGTG CCCTTGTGGC 
CACGGGCTCA TGGTCCTGCT GCGCGTGCCC TGTTCACCCC TGGCAGCCGT GGCCTATGAG 
CGACTGGCCC ACGTGCGGGC TCGCTTGGCT CTGGAGGAAC ACTTTGAGAT CATCCTGGGC 
CACCCCAGCT CCGGTATCAC TGTAGGCAAG CACTTTGTGA AGCAGCTCAA GATGTGGCAG 
AAAATTGAGG ATGCAGAATG GAGGCCTCAG ACGTACTTAG AGTTGGAGGG CCTGCCCTGC 
ATCCTGATCT TCAGTGGGAT GGACCCTCAT GGGGAGTCAC TGCCCCGGTC TCTGAGGTAC 
TGTGACCTGC GTCTAATAAA CTCCTCCTGC CTGGTGAGGA CAGCCCTGGA GCAGGAGCTG 
GGTCTGGCTG CCTACTTTGT TAGCAATGAT ATCCCCTTGG AGAAGGGGCC CAAGAACGAG 
GCCTTGGAGA GTGATGGGGA GAAACTGAGC AGCACCGATG AAGACGAGGA GGCGGGGACA 
GAAGGGTGTT TTGAAGCAGG CTCTACGTCA GAGCAGAGGG GCCCTGTGAA GAGGGAACGC 
TCCCACTCCC ACGACTCTGC ATCGTCATCC CTCTCCTCCA GAGCATCTGG CTCGGTGCTC 
TATGGTGAGT CCCTGGCCCA GCCCTCAGGA CCTCCTCAGG GAGAGCTTAC CAGATCACCT 
CCATCCTGTG GCCCTGCAGA GGAAGGCAGG GCCCCTGGGG AAATCCAGCG CCTCCGAGTG 
AGTCAGGGGT CTACAGTCAT CAGTAGGCAC AGCCCTGGCC TGGTCCCACA GCCTGATTCT 
AGCCTCAGGA CTGGTCGGAG GAGCTTGCAG GTGCCAGCCG CCCCGTCCTC CCAGCTGTCC 
TCCTCCTCGG GCTCATCATC CACATGTGCG GTGCCCACTG CCAATGTGCT GGTCCTTCAA 
GCCTCTCAGT GCTCCATGGC CAAGGCCTGC CGGCAGCCAC CCATCGTCTT CCTGCCGAAG 
CTTGTGTACG ACATGCTCCT GTCCACCGAC AGCAGCGGCT TGCCCAAGTC TGCCTCCCTC 
CTGCCTTCCC CTTCAGTCAT GTGGACCAGC TCCTTCCGCC CCCTGCTCAG CAAGATGATG 
ACATCAACTG AGCAGTCTCT CTACTACCGG CAGTGGACAG TGCCCCGGCC CAGCCACATG 
GACTACGGCA ACAGAGCTGA GGGCCGTGTG GACAGCTTCC ACCCCCGCAG GCTGCTGCTC 
AGTGGGCCAC CTCAGATTGG GAAGACAGGT GCCTACCTGC AGTTCCTCAG TATCTTGTCC 
AGGATGCTGA TTCGGCTGAC AGAAGTGGAT GTTTACGACG AAGAAGAGAT CAATACCAGC 
TTCCGAGAGG AATCGGAGTG GCGTTACCTC CAGCTGGCCG ACCCCTGGCC AGACCTGGAG 
CTCTTCCAGA AGATGCCTTT TGATTACATC ATTCATGACC CGAAGTATGA GGACGCCAGT 
CTGATTTGTT CACACCCTCA GACCATCAAG AGTGAAGACA GAGGGATGTC CAGGAAGCCG 
GAGGACCTGT ATGTGCGGCG TCAGACGGCA CGTATGAGAC TGTCCAAGTA TGCAGCATAC 
AACACGTACC ACCACTGTGA GCAGTGCCAG CAGTACATGG GCTTCCACCC CCACTACCAG 
CTCTCTGAGT CCACCCTGCA CGTCTTTGCC TTCTCTTGCT CCATGCTAGG AGAGGAGGTC 
CAACTTCACT TCATCATCCC CAAGTCCAAG GAGCACCACT TTGTTTTCAG CCAACCCGGA 
GGCCAGCTGG AGAGCATGCG CTTGCCCCTC GTCACAGACA AGAGTCATGA ACACATCAAA 
AGCCCAACCT TCACACCGAC AACGGGCCGG CACGAGCACG GGCTCTTTAA TTTGTACCAT 
GCGATGGATG GAGCCAACCA CCTACACGTG CTGGTGGTCA AGGAGTACGA AATGGCCATT 
TATAAGAAAT ACTGGCCCAA CCACATCATG CTTGTCCTCC CAAGCATCTT TAACAGTGCT 
GGTGTCGGGG CTGCCCACTT CCTGATCAAA GAGCTTTGTT ACCACAACCT GGAGCTGGAG 
CGGAACCGGC AGGAGGAGCT GGGGGTCAAG CCTCAGGACG TCTGGCCCTT CATCGTCATT 
GCTGATGACT CCTGTGTGAT GTGGAATGTA GCAGATGTGG ACTGTGCCGG AGAACGGAGC 
AGGGAGTTTT CCTGGTCGGA GAGGAATGTC TCTCTGAAAT ACATCATGCT ACACATTGAG 
GCGTCCCCTA ACATCACACA CTATGCCCTG CTCGGCATGC GGAAGTGGGC CAGCAAGACC 
CGGGGCCGAG AGGTACAGGA GCCTTTTTCA CGATGTCATG TGCACGACTT CATCATCCTC 
AACGTGGACT TGACCCAGAA TGTACAGTAC AACCAGAACC GGTTTACATG TGACGATGTA 
GACTTCAACC TCCGTGTGCA CAGTGCTGGC CTCCTGCTCT GCCGGTTCAA TCGCTTCAGT 
GTCATGAAGA AGCAGATTGC AGTGGGTGGG CACAGGTCTT TCCACATCAC ATCCAAGGTA 
TCTGACAGCT CTGTGGCCAT TGTGCCGTCC CAGTACATCT GTGCCCCCGA CAGTAAGCAT 
ACGTTCCTGG CAGCTCCCGC CCAGCTCCTG CTGGAGAAAT TCCTCCAATA CCATAGCCAT 
CGCTTCTTCC CGCTGTCTCT GAAGAATCAC AGCCACCCCG TGTTGTCTGT AGACTGCTAC 
CTGAACCTGG GACCTCAGAT TTCTGTTTGT TATGTGAGCT CCAGGCCCCA CTCCTTAAAC 
ATCAGCTGCT CTGACATGGT GTTCAGCGGG CTCCTGCTGT ACCTCTGTGA CTCTTTTGTG 
GGGGCTAGCT TCTTGAAGAA GTTCCATTTT CTGAAAGGAG CCACCTTGTG TGTGATCTGC 
CAAGACCGGA ACTCACTTCG CCAGACGGTG GTCCGCCTGG AGCTAGAAGA TGAGTGGCAG 
TTCCGGCTGC GGGATGAGTT CCAGACAGCC AATGCCAAGG AAGACAGGCC GCTCTTTTTC 
CTCACCGCAC GGCACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515163.1
CDS	708..6488
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTNSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515100.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515100.2

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAATTCCAG CACCCAGAAT 
CTGGACCTGG GGTCCTTCGA GAAGGTGGAC TTTCTCATTT GCATTCCACC GTCAGAGGTG 
ACCTACCAGC AGACCGTGTT CCACGTCTGG CATTCAGGGG TTCTGCTGGA GCTGGGCCTG 
GAGAAAGAGC CTGTGACCAA GCAGAGGGCG GAGCAACACG TTCTGAAACT AGACACGGAG 
GCCCAGGCAA GATTTAAGGC CTTTCTGCAA AACTCCTTCC AGAACCCACA TACGCTCTTT 
GTCCTCATCC ACGACCACGC CCACTGGGAT CTAGTGAGCA GTGCTGTTCA TAACATCTAC 
TCTCAAAGTG ACCCATCTGT GGGGTTAGTG GACAGACTGC TCAACTGCAG GGAGGTGAAA 
GAGGCGCCCA ACATTGTGAC CCTCCATGTG ACCTCTTTCC CGTATGCGCT GCAGACTCAG 
CACACCCTCA TTAGCCCATA CAATGAGATC CATTGGCCCA TCTCCTTCAG TAATGGAGTG 
GACCTGTATC ATGAGAGTAA GAAGTACTTC GGGCTGTCTG AGTTTATTGA CTCCACCCTG 
TCAGGACACA GTCTTCCTCT GCTCCGATAT GACAGTTCCT TTGAGGCCAT GGTCACTGCA 
TTGGGGAAAA GGTTCCCCCG CCTGCACAGC GCGGTCATCA GGACCTTTGT TCTTGTGCAG 
CACTATGCGG CCGCCATGAT GGCCGTGAGT GGCCTCCCGC AGATGAAGAA CCATACATCA 
GTGGAGACCC TAGAGATCAC CCAGAACCTC CTCAACTCCC CGAAGCAGTG CCCTTGTGGC 
CACGGGCTCA TGGTCCTGCT GCGCGTGCCC TGTTCACCCC TGGCAGCCGT GGCCTATGAG 
CGACTGGCCC ACGTGCGGGC TCGCTTGGCT CTGGAGGAAC ACTTTGAGAT CATCCTGGGC 
CACCCCAGCT CCGGTATCAC TGTAGGCAAG CACTTTGTGA AGCAGCTCAA GATGTGGCAG 
AAAATTGAGG ATGCAGAATG GAGGCCTCAG ACGTACTTAG AGTTGGAGGG CCTGCCCTGC 
ATCCTGATCT TCAGTGGGAT GGACCCTCAT GGGGAGTCAC TGCCCCGGTC TCTGAGGTAC 
TGTGACCTGC GTCTAATAAA CTCCTCCTGC CTGGTGAGGA CAGCCCTGGA GCAGGAGCTG 
GGTCTGGCTG CCTACTTTGT TAGCAATGAT ATCCCCTTGG AGAAGGGGCC CAAGAACGAG 
GCCTTGGAGA GTGATGGGGA GAAACTGAGC AGCACCGATG AAGACGAGGA GGCGGGGACA 
GAAGGGTGTT TTGAAGCAGG CTCTACGTCA GAGCAGAGGG GCCCTGTGAA GAGGGAACGC 
TCCCACTCCC ACGACTCTGC ATCGTCATCC CTCTCCTCCA GAGCATCTGG CTCGGTGCTC 
TATGGTGAGT CCCTGGCCCA GCCCTCAGGA CCTCCTCAGG GAGAGCTTAC CAGATCACCT 
CCATCCTGTG GCCCTGCAGA GGAAGGCAGG GCCCCTGGGG AAATCCAGCG CCTCCGAGTG 
AGTCAGGGGT CTACAGTCAT CAGTAGGCAC AGCCCTGGCC TGGTCCCACA GCCTGATTCT 
AGCCTCAGGA CTGGTCGGAG GAGCTTGCAG GTGCCAGCCG CCCCGTCCTC CCAGCTGTCC 
TCCTCCTCGG GCTCATCATC CACATGTGCG GTGCCCACTG CCAATGTGCT GGTCCTTCAA 
GCCTCTCAGT GCTCCATGGC CAAGGCCTGC CGGCAGCCAC CCATCGTCTT CCTGCCGAAG 
CTTGTGTACG ACATGCTCCT GTCCACCGAC AGCAGCGGCT TGCCCAAGTC TGCCTCCCTC 
CTGCCTTCCC CTTCAGTCAT GTGGACCAGC TCCTTCCGCC CCCTGCTCAG CAAGATGATG 
ACATCAACTG AGCAGTCTCT CTACTACCGG CAGTGGACAG TGCCCCGGCC CAGCCACATG 
GACTACGGCA ACAGAGCTGA GGGCCGTGTG GACAGCTTCC ACCCCCGCAG GCTGCTGCTC 
AGTGGGCCAC CTCAGATTGG GAAGACAGGT GCCTACCTGC AGTTCCTCAG TATCTTGTCC 
AGGATGCTGA TTCGGCTGAC AGAAGTGGAT GTTTACGACG AAGAAGAGAT CAATACCAGC 
TTCCGAGAGG AATCGGAGTG GCGTTACCTC CAGCTGGCCG ACCCCTGGCC AGACCTGGAG 
CTCTTCCAGA AGATGCCTTT TGATTACATC ATTCATGACC CGAAGTATGA GGACGCCAGT 
CTGATTTGTT CACACCCTCA GACCATCAAG AGTGAAGACA GAGGGATGTC CAGGAAGCCG 
GAGGACCTGT ATGTGCGGCG TCAGACGGCA CGTATGAGAC TGTCCAAGTA TGCAGCATAC 
AACACGTACC ACCACTGTGA GCAGTGCCAG CAGTACATGG GCTTCCACCC CCACTACCAG 
CTCTCTGAGT CCACCCTGCA CGTCTTTGCC TTCTCTTGCT CCATGCTAGG AGAGGAGGTC 
CAACTTCACT TCATCATCCC CAAGTCCAAG GAGCACCACT TTGTTTTCAG CCAACCCGGA 
GGCCAGCTGG AGAGCATGCG CTTGCCCCTC GTCACAGACA AGAGTCATGA ACACATCAAA 
AGCCCAACCT TCACACCGAC AACGGGCCGG CACGAGCACG GGCTCTTTAA TTTGTACCAT 
GCGATGGATG GAGCCAACCA CCTACACGTG CTGGTGGTCA AGGAGTACGA AATGGCCATT 
TATAAGAAAT ACTGGCCCAA CCACATCATG CTTGTCCTCC CAAGCATCTT TAACAGTGCT 
GGTGTCGGGG CTGCCCACTT CCTGATCAAA GAGCTTTGTT ACCACAACCT GGAGCTGGAG 
CGGAACCGGC AGGAGGAGCT GGGGGTCAAG CCTCAGGACG TCTGGCCCTT CATCGTCATT 
GCTGATGACT CCTGTGTGAT GTGGAATGTA GCAGATGTGG ACTGTGCCGG AGAACGGAGC 
AGGGAGTTTT CCTGGTCGGA GAGGAATGTC TCTCTGAAAT ACATCATGCT ACACATTGAG 
GCGTCCCCTA ACATCACACA CTATGCCCTG CTCGGCATGC GGAAGTGGGC CAGCAAGACC 
CGGGGCCGAG AGGTACAGGA GCCTTTTTCA CGATGTCATG TGCACGACTT CATCATCCTC 
AACGTGGACT TGACCCAGAA TGTACAGTAC AACCAGAACC GGTTTACATG TGACGATGTA 
GACTTCAACC TCCGTGTGCA CAGTGCTGGC CTCCTGCTCT GCCGGTTCAA TCGCTTCAGT 
GTCATGAAGA AGCAGATTGC AGTGGGTGGG CACAGGTCTT TCCACATCAC ATCCAAGGTA 
TCTGACAGCT CTGTGGCCAT TGTGCCGTCC CAGTACATCT GTGCCCCCGA CAGTAAGCAT 
ACGTTCCTGG CAGCTCCCGC CCAGCTCCTG CTGGAGAAAT TCCTCCAATA CCATAGCCAT 
CGCTTCTTCC CGCTGTCTCT GAAGAATCAC AGCCACCCCG TGTTGTCTGT AGACTGCTAC 
CTGAACCTGG GACCTCAGAT TTCTGTTTGT TATGTGAGCT CCAGGCCCCA CTCCTTAAAC 
ATCAGCTGCT CTGACATGGT GTTCAGCGGG CTCCTGCTGT ACCTCTGTGA CTCTTTTGTG 
GGGGCTAGCT TCTTGAAGAA GTTCCATTTT CTGAAAGGAG CCACCTTGTG TGTGATCTGC 
CAAGACCGGA ACTCACTTCG CCAGACGGTG GTCCGCCTGG AGCTAGAAGA TGAGTGGCAG 
TTCCGGCTGC GGGATGAGTT CCAGACAGCC AATGCCAAGG AAGACAGGCC GCTCTTTTTC 
CTCACCGCAC GGCACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515094.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515157.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X2, mRNA.

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CloneID	OMu10233
Clone ID Related Accession (Same CDS sequence)	XM_006515093.3 , XM_006515094.3 , NM_015764.4 , XM_006515097.3 , XM_006515098.3 , XM_006515093.2 , XM_006515098.2 , XM_006515095.3 , XM_006515096.3 , XM_006515096.2
Accession Version	XM_006515094.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515094.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515157.1
CDS	433..6297
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515094.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515094.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515097.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515160.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X5, mRNA.

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CloneID	OMu10233
Clone ID Related Accession (Same CDS sequence)	XM_006515093.3 , XM_006515094.3 , NM_015764.4 , XM_006515097.3 , XM_006515098.3 , XM_006515093.2 , XM_006515098.2 , XM_006515095.3 , XM_006515096.3 , XM_006515096.2
Accession Version	XM_006515097.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515097.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515160.1
CDS	334..6198
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515097.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515097.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515093.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515156.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X1, mRNA.

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CloneID	OMu10233
Clone ID Related Accession (Same CDS sequence)	XM_006515093.3 , XM_006515094.3 , NM_015764.4 , XM_006515097.3 , XM_006515098.3 , XM_006515093.2 , XM_006515098.2 , XM_006515095.3 , XM_006515096.3 , XM_006515096.2
Accession Version	XM_006515093.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006515093.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515156.1
CDS	708..6572
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515093.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515093.2

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515098.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515161.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X6, mRNA.

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CloneID	OMu10233
Clone ID Related Accession (Same CDS sequence)	XM_006515093.3 , XM_006515094.3 , NM_015764.4 , XM_006515097.3 , XM_006515098.3 , XM_006515093.2 , XM_006515098.2 , XM_006515095.3 , XM_006515096.3 , XM_006515096.2
Accession Version	XM_006515098.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006515098.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515161.1
CDS	499..6363
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515098.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515098.2

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515095.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515158.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X3, mRNA.

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CloneID	OMu10233
Clone ID Related Accession (Same CDS sequence)	XM_006515093.3 , XM_006515094.3 , NM_015764.4 , XM_006515097.3 , XM_006515098.3 , XM_006515093.2 , XM_006515098.2 , XM_006515095.3 , XM_006515096.3 , XM_006515096.2
Accession Version	XM_006515095.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515158.1
CDS	386..6250
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515095.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515095.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515096.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515159.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X4, mRNA.

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CloneID	OMu10233
Clone ID Related Accession (Same CDS sequence)	XM_006515093.3 , XM_006515094.3 , NM_015764.4 , XM_006515097.3 , XM_006515098.3 , XM_006515093.2 , XM_006515098.2 , XM_006515095.3 , XM_006515096.3 , XM_006515096.2
Accession Version	XM_006515096.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006515096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515159.1
CDS	609..6473
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515096.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515096.2

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGATTTCTGT TTGTTATGTG 
AGCTCCAGGC CCCACTCCTT AAACATCAGC TGCTCTGACA TGGTGTTCAG CGGGCTCCTG 
CTGTACCTCT GTGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGTGTGAT CTGCCAAGAC CGGAACTCAC TTCGCCAGAC GGTGGTCCGC 
CTGGAGCTAG AAGATGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCGCTCTT TTTCCTCACC GCACGGCACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515099.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515162.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X7, mRNA.

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CloneID	OMu47321
Clone ID Related Accession (Same CDS sequence)	XM_006515099.2 , XM_006515099.3
Accession Version	XM_006515099.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006515099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGTGCTGTTC ATAACATCTA CTCTCAAAGT GACCCATCTG TGGGGTTAGT GGACAGACTG 
CTCAACTGCA GGGAGGTGAA AGAGGCGCCC AACATTGTGA CCCTCCATGT GACCTCTTTC 
CCGTATGCGC TGCAGACTCA GCACACCCTC ATTAGCCCAT ACAATGAGAT CCATTGGCCC 
ATCTCCTTCA GTAATGGAGT GGACCTGTAT CATGAGAGTA AGAAGTACTT CGGGCTGTCT 
GAGTTTATTG ACTCCACCCT GTCAGGACAC AGTCTTCCTC TGCTCCGATA TGACAGTTCC 
TTTGAGGCCA TGGTCACTGC ATTGGGGAAA AGGTTCCCCC GCCTGCACAG CGCGGTCATC 
AGGACCTTTG TTCTTGTGCA GCACTATGCG GCCGCCATGA TGGCCGTGAG TGGCCTCCCG 
CAGATGAAGA ACCATACATC AGTGGAGACC CTAGAGATCA CCCAGAACCT CCTCAACTCC 
CCGAAGCAGT GCCCTTGTGG CCACGGGCTC ATGGTCCTGC TGCGCGTGCC CTGTTCACCC 
CTGGCAGCCG TGGCCTATGA GCGACTGGCC CACGTGCGGG CTCGCTTGGC TCTGGAGGAA 
CACTTTGAGA TCATCCTGGG CCACCCCAGC TCCGGTATCA CTGTAGGCAA GCACTTTGTG 
AAGCAGCTCA AGATGTGGCA GAAAATTGAG GATGCAGAAT GGAGGCCTCA GACGTACTTA 
GAGTTGGAGG GCCTGCCCTG CATCCTGATC TTCAGTGGGA TGGACCCTCA TGGGGAGTCA 
CTGCCCCGGT CTCTGAGGTA CTGTGACCTG CGTCTAATAA ACTCCTCCTG CCTGGTGAGG 
ACAGCCCTGG AGCAGGAGCT GGGTCTGGCT GCCTACTTTG TTAGCAATGA TATCCCCTTG 
GAGAAGGGGC CCAAGAACGA GGCCTTGGAG AGTGATGGGG AGAAACTGAG CAGCACCGAT 
GAAGACGAGG AGGCGGGGAC AGAAGGGTGT TTTGAAGCAG GCTCTACGTC AGAGCAGAGG 
GGCCCTGTGA AGAGGGAACG CTCCCACTCC CACGACTCTG CATCGTCATC CCTCTCCTCC 
AGAGCATCTG GCTCGGTGCT CTATGGTGAG TCCCTGGCCC AGCCCTCAGG ACCTCCTCAG 
GGAGAGCTTA CCAGATCACC TCCATCCTGT GGCCCTGCAG AGGAAGGCAG GGCCCCTGGG 
GAAATCCAGC GCCTCCGAGT GAGTCAGGGG TCTACAGTCA TCAGTAGGCA CAGCCCTGGC 
CTGGTCCCAC AGCCTGATTC TAGCCTCAGG ACTGGTCGGA GGAGCTTGCA GGTGCCAGCC 
GCCCCGTCCT CCCAGCTGTC CTCCTCCTCG GGCTCATCAT CCACATGTGC GGTGCCCACT 
GCCAATGTGC TGGTCCTTCA AGCCTCTCAG TGCTCCATGG CCAAGGCCTG CCGGCAGCCA 
CCCATCGTCT TCCTGCCGAA GCTTGTGTAC GACATGCTCC TGTCCACCGA CAGCAGCGGC 
TTGCCCAAGT CTGCCTCCCT CCTGCCTTCC CCTTCAGTCA TGTGGACCAG CTCCTTCCGC 
CCCCTGCTCA GCAAGATGAT GACATCAACT GAGCAGTCTC TCTACTACCG GCAGTGGACA 
GTGCCCCGGC CCAGCCACAT GGACTACGGC AACAGAGCTG AGGGCCGTGT GGACAGCTTC 
CACCCCCGCA GGCTGCTGCT CAGTGGGCCA CCTCAGATTG GGAAGACAGG TGCCTACCTG 
CAGTTCCTCA GTATCTTGTC CAGGATGCTG ATTCGGCTGA CAGAAGTGGA TGTTTACGAC 
GAAGAAGAGA TCAATACCAG CTTCCGAGAG GAATCGGAGT GGCGTTACCT CCAGCTGGCC 
GACCCCTGGC CAGACCTGGA GCTCTTCCAG AAGATGCCTT TTGATTACAT CATTCATGAC 
CCGAAGTATG AGGACGCCAG TCTGATTTGT TCACACCCTC AGACCATCAA GAGTGAAGAC 
AGAGGGATGT CCAGGAAGCC GGAGGACCTG TATGTGCGGC GTCAGACGGC ACGTATGAGA 
CTGTCCAAGT ATGCAGCATA CAACACGTAC CACCACTGTG AGCAGTGCCA GCAGTACATG 
GGCTTCCACC CCCACTACCA GCTCTCTGAG TCCACCCTGC ACGTCTTTGC CTTCTCTTGC 
TCCATGCTAG GAGAGGAGGT CCAACTTCAC TTCATCATCC CCAAGTCCAA GGAGCACCAC 
TTTGTTTTCA GCCAACCCGG AGGCCAGCTG GAGAGCATGC GCTTGCCCCT CGTCACAGAC 
AAGAGTCATG AACACATCAA AAGCCCAACC TTCACACCGA CAACGGGCCG GCACGAGCAC 
GGGCTCTTTA ATTTGTACCA TGCGATGGAT GGAGCCAACC ACCTACACGT GCTGGTGGTC 
AAGGAGTACG AAATGGCCAT TTATAAGAAA TACTGGCCCA ACCACATCAT GCTTGTCCTC 
CCAAGCATCT TTAACAGTGC TGGTGTCGGG GCTGCCCACT TCCTGATCAA AGAGCTTTGT 
TACCACAACC TGGAGCTGGA GCGGAACCGG CAGGAGGAGC TGGGGGTCAA GCCTCAGGAC 
GTCTGGCCCT TCATCGTCAT TGCTGATGAC TCCTGTGTGA TGTGGAATGT AGCAGATGTG 
GACTGTGCCG GAGAACGGAG CAGGGAGTTT TCCTGGTCGG AGAGGAATGT CTCTCTGAAA 
TACATCATGC TACACATTGA GGCGTCCCCT AACATCACAC ACTATGCCCT GCTCGGCATG 
CGGAAGTGGG CCAGCAAGAC CCGGGGCCGA GAGGTACAGG AGCCTTTTTC ACGATGTCAT 
GTGCACGACT TCATCATCCT CAACGTGGAC TTGACCCAGA ATGTACAGTA CAACCAGAAC 
CGGTTTACAT GTGACGATGT AGACTTCAAC CTCCGTGTGC ACAGTGCTGG CCTCCTGCTC 
TGCCGGTTCA ATCGCTTCAG TGTCATGAAG AAGCAGATTG CAGTGGGTGG GCACAGGTCT 
TTCCACATCA CATCCAAGGT ATCTGACAGC TCTGTGGCCA TTGTGCCGTC CCAGTACATC 
TGTGCCCCCG ACAGTAAGCA TACGTTCCTG GCAGCTCCCG CCCAGCTCCT GCTGGAGAAA 
TTCCTCCAAT ACCATAGCCA TCGCTTCTTC CCGCTGTCTC TGAAGAATCA CAGCCACCCC 
GTGTTGTCTG TAGACTGCTA CCTGAACCTG GGACCTCAGA TTTCTGTTTG TTATGTGAGC 
TCCAGGCCCC ACTCCTTAAA CATCAGCTGC TCTGACATGG TGTTCAGCGG GCTCCTGCTG 
TACCTCTGTG ACTCTTTTGT GGGGGCTAGC TTCTTGAAGA AGTTCCATTT TCTGAAAGGA 
GCCACCTTGT GTGTGATCTG CCAAGACCGG AACTCACTTC GCCAGACGGT GGTCCGCCTG 
GAGCTAGAAG ATGAGTGGCA GTTCCGGCTG CGGGATGAGT TCCAGACAGC CAATGCCAAG 
GAAGACAGGC CGCTCTTTTT CCTCACCGCA CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515162.1
CDS	708..6569
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNISCSDMVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006515099.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515099.2

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGTGCTGTTC ATAACATCTA CTCTCAAAGT GACCCATCTG TGGGGTTAGT GGACAGACTG 
CTCAACTGCA GGGAGGTGAA AGAGGCGCCC AACATTGTGA CCCTCCATGT GACCTCTTTC 
CCGTATGCGC TGCAGACTCA GCACACCCTC ATTAGCCCAT ACAATGAGAT CCATTGGCCC 
ATCTCCTTCA GTAATGGAGT GGACCTGTAT CATGAGAGTA AGAAGTACTT CGGGCTGTCT 
GAGTTTATTG ACTCCACCCT GTCAGGACAC AGTCTTCCTC TGCTCCGATA TGACAGTTCC 
TTTGAGGCCA TGGTCACTGC ATTGGGGAAA AGGTTCCCCC GCCTGCACAG CGCGGTCATC 
AGGACCTTTG TTCTTGTGCA GCACTATGCG GCCGCCATGA TGGCCGTGAG TGGCCTCCCG 
CAGATGAAGA ACCATACATC AGTGGAGACC CTAGAGATCA CCCAGAACCT CCTCAACTCC 
CCGAAGCAGT GCCCTTGTGG CCACGGGCTC ATGGTCCTGC TGCGCGTGCC CTGTTCACCC 
CTGGCAGCCG TGGCCTATGA GCGACTGGCC CACGTGCGGG CTCGCTTGGC TCTGGAGGAA 
CACTTTGAGA TCATCCTGGG CCACCCCAGC TCCGGTATCA CTGTAGGCAA GCACTTTGTG 
AAGCAGCTCA AGATGTGGCA GAAAATTGAG GATGCAGAAT GGAGGCCTCA GACGTACTTA 
GAGTTGGAGG GCCTGCCCTG CATCCTGATC TTCAGTGGGA TGGACCCTCA TGGGGAGTCA 
CTGCCCCGGT CTCTGAGGTA CTGTGACCTG CGTCTAATAA ACTCCTCCTG CCTGGTGAGG 
ACAGCCCTGG AGCAGGAGCT GGGTCTGGCT GCCTACTTTG TTAGCAATGA TATCCCCTTG 
GAGAAGGGGC CCAAGAACGA GGCCTTGGAG AGTGATGGGG AGAAACTGAG CAGCACCGAT 
GAAGACGAGG AGGCGGGGAC AGAAGGGTGT TTTGAAGCAG GCTCTACGTC AGAGCAGAGG 
GGCCCTGTGA AGAGGGAACG CTCCCACTCC CACGACTCTG CATCGTCATC CCTCTCCTCC 
AGAGCATCTG GCTCGGTGCT CTATGGTGAG TCCCTGGCCC AGCCCTCAGG ACCTCCTCAG 
GGAGAGCTTA CCAGATCACC TCCATCCTGT GGCCCTGCAG AGGAAGGCAG GGCCCCTGGG 
GAAATCCAGC GCCTCCGAGT GAGTCAGGGG TCTACAGTCA TCAGTAGGCA CAGCCCTGGC 
CTGGTCCCAC AGCCTGATTC TAGCCTCAGG ACTGGTCGGA GGAGCTTGCA GGTGCCAGCC 
GCCCCGTCCT CCCAGCTGTC CTCCTCCTCG GGCTCATCAT CCACATGTGC GGTGCCCACT 
GCCAATGTGC TGGTCCTTCA AGCCTCTCAG TGCTCCATGG CCAAGGCCTG CCGGCAGCCA 
CCCATCGTCT TCCTGCCGAA GCTTGTGTAC GACATGCTCC TGTCCACCGA CAGCAGCGGC 
TTGCCCAAGT CTGCCTCCCT CCTGCCTTCC CCTTCAGTCA TGTGGACCAG CTCCTTCCGC 
CCCCTGCTCA GCAAGATGAT GACATCAACT GAGCAGTCTC TCTACTACCG GCAGTGGACA 
GTGCCCCGGC CCAGCCACAT GGACTACGGC AACAGAGCTG AGGGCCGTGT GGACAGCTTC 
CACCCCCGCA GGCTGCTGCT CAGTGGGCCA CCTCAGATTG GGAAGACAGG TGCCTACCTG 
CAGTTCCTCA GTATCTTGTC CAGGATGCTG ATTCGGCTGA CAGAAGTGGA TGTTTACGAC 
GAAGAAGAGA TCAATACCAG CTTCCGAGAG GAATCGGAGT GGCGTTACCT CCAGCTGGCC 
GACCCCTGGC CAGACCTGGA GCTCTTCCAG AAGATGCCTT TTGATTACAT CATTCATGAC 
CCGAAGTATG AGGACGCCAG TCTGATTTGT TCACACCCTC AGACCATCAA GAGTGAAGAC 
AGAGGGATGT CCAGGAAGCC GGAGGACCTG TATGTGCGGC GTCAGACGGC ACGTATGAGA 
CTGTCCAAGT ATGCAGCATA CAACACGTAC CACCACTGTG AGCAGTGCCA GCAGTACATG 
GGCTTCCACC CCCACTACCA GCTCTCTGAG TCCACCCTGC ACGTCTTTGC CTTCTCTTGC 
TCCATGCTAG GAGAGGAGGT CCAACTTCAC TTCATCATCC CCAAGTCCAA GGAGCACCAC 
TTTGTTTTCA GCCAACCCGG AGGCCAGCTG GAGAGCATGC GCTTGCCCCT CGTCACAGAC 
AAGAGTCATG AACACATCAA AAGCCCAACC TTCACACCGA CAACGGGCCG GCACGAGCAC 
GGGCTCTTTA ATTTGTACCA TGCGATGGAT GGAGCCAACC ACCTACACGT GCTGGTGGTC 
AAGGAGTACG AAATGGCCAT TTATAAGAAA TACTGGCCCA ACCACATCAT GCTTGTCCTC 
CCAAGCATCT TTAACAGTGC TGGTGTCGGG GCTGCCCACT TCCTGATCAA AGAGCTTTGT 
TACCACAACC TGGAGCTGGA GCGGAACCGG CAGGAGGAGC TGGGGGTCAA GCCTCAGGAC 
GTCTGGCCCT TCATCGTCAT TGCTGATGAC TCCTGTGTGA TGTGGAATGT AGCAGATGTG 
GACTGTGCCG GAGAACGGAG CAGGGAGTTT TCCTGGTCGG AGAGGAATGT CTCTCTGAAA 
TACATCATGC TACACATTGA GGCGTCCCCT AACATCACAC ACTATGCCCT GCTCGGCATG 
CGGAAGTGGG CCAGCAAGAC CCGGGGCCGA GAGGTACAGG AGCCTTTTTC ACGATGTCAT 
GTGCACGACT TCATCATCCT CAACGTGGAC TTGACCCAGA ATGTACAGTA CAACCAGAAC 
CGGTTTACAT GTGACGATGT AGACTTCAAC CTCCGTGTGC ACAGTGCTGG CCTCCTGCTC 
TGCCGGTTCA ATCGCTTCAG TGTCATGAAG AAGCAGATTG CAGTGGGTGG GCACAGGTCT 
TTCCACATCA CATCCAAGGT ATCTGACAGC TCTGTGGCCA TTGTGCCGTC CCAGTACATC 
TGTGCCCCCG ACAGTAAGCA TACGTTCCTG GCAGCTCCCG CCCAGCTCCT GCTGGAGAAA 
TTCCTCCAAT ACCATAGCCA TCGCTTCTTC CCGCTGTCTC TGAAGAATCA CAGCCACCCC 
GTGTTGTCTG TAGACTGCTA CCTGAACCTG GGACCTCAGA TTTCTGTTTG TTATGTGAGC 
TCCAGGCCCC ACTCCTTAAA CATCAGCTGC TCTGACATGG TGTTCAGCGG GCTCCTGCTG 
TACCTCTGTG ACTCTTTTGT GGGGGCTAGC TTCTTGAAGA AGTTCCATTT TCTGAAAGGA 
GCCACCTTGT GTGTGATCTG CCAAGACCGG AACTCACTTC GCCAGACGGT GGTCCGCCTG 
GAGCTAGAAG ATGAGTGGCA GTTCCGGCTG CGGGATGAGT TCCAGACAGC CAATGCCAAG 
GAAGACAGGC CGCTCTTTTT CCTCACCGCA CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006515101.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515164.1 Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X9, mRNA.

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CloneID	OMu47322
Clone ID Related Accession (Same CDS sequence)	XM_006515101.4 , XM_006515101.3
Accession Version	XM_006515101.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GREB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515101.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGGAGCCACC TTGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515164.1
CDS	709..6288
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLEPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISSEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRGRQNSTNTCSSSLFPPLESSGSLAAFPTEPVPGTNPSVPVGAQQAGPASDHPSVTTATGPAVFNGKDSPKHPQLVKSSLSALPRPSALGILPNSGPPKKRHKGWSPESKSTTDGGFIQGGGNRAKHEGTSIPCVPQAGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLARAYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGTFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPVCTSYHQEPRGVRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISFSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSRASGSVLYGESLAQPSGPPQGELTRSPPSCGPAEEGRAPGEIQRLRVSQGSTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSGSSSTCAVPTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTSFREESEWRYLQLADPWPDLELFQKMPFDYIIHDPKYEDASLICSHPQTIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEVQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDVWPFIVIADDSCVMWNVADVDCAGERSREFSWSERNVSLKYIMLHIEASPNITHYALLGMRKWASKTRGREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVSDSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQEPPCV

Target ORF information:

RefSeq Version	XM_006515101.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gene regulated by estrogen in breast cancer protein (Greb1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515101.3

ORF Insert Sequence:

ATGGGCAATT CCTATGCTGG CCAGCTAAAG AGCACACGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCTTACG CTCCAACACC CTGGTGCCCC GGCCCATTTT CTCGCAGCTG 
TATCTGGAGG CTGAGCAGCA GCTGTCATCG TTAGAAGGCG GCAGCCGAGC AGACAATGAG 
GAAGAAGAAG AAGATGGAGA AGGAGGGTTA GAACCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG CTGCTGCACC ACGGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAGTGAGCCC ATTGAAGTCC CTGCAGGCTT CCTCCTGGTG 
GGTGCTAAGT CTCCCAGCCT GCCGGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTTGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA TTTCACCGAG TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACCCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGGAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCCGAGT TCAGAGGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTCTTCCCA CCGCTGGAGA GCTCGGGGTC CCTGGCTGCC 
TTCCCCACTG AGCCTGTTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGACC ACAGCAACAG GTCCTGCAGT CTTCAATGGC 
AAGGATTCCC CCAAGCACCC ACAGCTGGTG AAGAGTAGCC TGTCTGCCCT GCCGCGCCCC 
TCAGCCTTGG GTATCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCGGAGTCTA AATCAACCAC GGATGGTGGC TTCATTCAGG GTGGTGGCAA CAGAGCCAAG 
CACGAAGGTA CCAGCATACC TTGTGTGCCC CAGGCTGGCT TGGTGGGACC AGCTTCAGTC 
ACCTTCCCGG TGGTGGCCTC TGGTGAACCA GTGTCTGTTC CGGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CATGGGAACT TTCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTGCTG TACACCTGCT ACCAGAACTC CCAGTCGCTT 
GCCAGGGCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA GGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAA CTCGCTGCAG ACCAGGTGCC GCTGATGGAG 
GACCTGGAGC AGATCTTCCT ACGCTCCTGG CGAGAGTCAC ACCTGACAGA GATAAGGCAG 
TACCAGCAGG CGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC ACCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCCGGCCTG GCCGCCAGCT CCTGTAATGA CAGTGTGCAT 
GTTATTGAGT GTGCTTACTC CCTGGCCGAG GGCCTCTCTG AGATGTTCCG GTTGCTCATC 
GAGGGGAAAC TCTCCAAGAC CAACTATGTG GTCATTATCT GTGCCTGCCG GAATGCCGCC 
ATTGACTCCT GTATTGCTGT CACAGGGAAA TACCAAGCCC GCATTCTCTC TGAGAGCCTT 
CTCAGTCCTG CTGAGTACCA GAGAGAAGTT CACTACGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGA CATTTTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAGACAGCTG TGCTGGATGC CAGTGGGGTC CCGGTGTGCA CAAGTTACCA CCAGGAGCCA 
CGGGGCGTCC GGCCCTTTCA GCTGGCGGTG GCACAGAAGC TCCTTTCCCA CGTGTGCTCC 
ATCGCAGATT CCAGCACCCA GAATCTGGAC CTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CACCGTCAGA GGTGACCTAC CAGCAGACCG TGTTCCACGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAGCCTGTGA CCAAGCAGAG GGCGGAGCAA 
CACGTTCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTACTCTCAA AGTGACCCAT CTGTGGGGTT AGTGGACAGA 
CTGCTCAACT GCAGGGAGGT GAAAGAGGCG CCCAACATTG TGACCCTCCA TGTGACCTCT 
TTCCCGTATG CGCTGCAGAC TCAGCACACC CTCATTAGCC CATACAATGA GATCCATTGG 
CCCATCTCCT TCAGTAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTCGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG ATATGACAGT 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTC 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCGCAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCGAAGC AGTGCCCTTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCA 
CCCCTGGCAG CCGTGGCCTA TGAGCGACTG GCCCACGTGC GGGCTCGCTT GGCTCTGGAG 
GAACACTTTG AGATCATCCT GGGCCACCCC AGCTCCGGTA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACGTAC 
TTAGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCACTGCCCC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAACTCCTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA GCTGGGTCTG GCTGCCTACT TTGTTAGCAA TGATATCCCC 
TTGGAGAAGG GGCCCAAGAA CGAGGCCTTG GAGAGTGATG GGGAGAAACT GAGCAGCACC 
GATGAAGACG AGGAGGCGGG GACAGAAGGG TGTTTTGAAG CAGGCTCTAC GTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGCTCCCAC TCCCACGACT CTGCATCGTC ATCCCTCTCC 
TCCAGAGCAT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG CCCAGCCCTC AGGACCTCCT 
CAGGGAGAGC TTACCAGATC ACCTCCATCC TGTGGCCCTG CAGAGGAAGG CAGGGCCCCT 
GGGGAAATCC AGCGCCTCCG AGTGAGTCAG GGGTCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGTC GGAGGAGCTT GCAGGTGCCA 
GCCGCCCCGT CCTCCCAGCT GTCCTCCTCC TCGGGCTCAT CATCCACATG TGCGGTGCCC 
ACTGCCAATG TGCTGGTCCT TCAAGCCTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATCG TCTTCCTGCC GAAGCTTGTG TACGACATGC TCCTGTCCAC CGACAGCAGC 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCAG TCATGTGGAC CAGCTCCTTC 
CGCCCCCTGC TCAGCAAGAT GATGACATCA ACTGAGCAGT CTCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAGGGCCG TGTGGACAGC 
TTCCACCCCC GCAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC AGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GACGAAGAAG AGATCAATAC CAGCTTCCGA GAGGAATCGG AGTGGCGTTA CCTCCAGCTG 
GCCGACCCCT GGCCAGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGACGC CAGTCTGATT TGTTCACACC CTCAGACCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCGGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCCA AGTATGCAGC ATACAACACG TACCACCACT GTGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GGTCCAACTT CACTTCATCA TCCCCAAGTC CAAGGAGCAC 
CACTTTGTTT TCAGCCAACC CGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTC ATGAACACAT CAAAAGCCCA ACCTTCACAC CGACAACGGG CCGGCACGAG 
CACGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCACCTACA CGTGCTGGTG 
GTCAAGGAGT ACGAAATGGC CATTTATAAG AAATACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGTGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTT 
TGTTACCACA ACCTGGAGCT GGAGCGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACGTCTGGC CCTTCATCGT CATTGCTGAT GACTCCTGTG TGATGTGGAA TGTAGCAGAT 
GTGGACTGTG CCGGAGAACG GAGCAGGGAG TTTTCCTGGT CGGAGAGGAA TGTCTCTCTG 
AAATACATCA TGCTACACAT TGAGGCGTCC CCTAACATCA CACACTATGC CCTGCTCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGGGC CGAGAGGTAC AGGAGCCTTT TTCACGATGT 
CATGTGCACG ACTTCATCAT CCTCAACGTG GACTTGACCC AGAATGTACA GTACAACCAG 
AACCGGTTTA CATGTGACGA TGTAGACTTC AACCTCCGTG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGTGTCATG AAGAAGCAGA TTGCAGTGGG TGGGCACAGG 
TCTTTCCACA TCACATCCAA GGTATCTGAC AGCTCTGTGG CCATTGTGCC GTCCCAGTAC 
ATCTGTGCCC CCGACAGTAA GCATACGTTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAATTCCTCC AATACCATAG CCATCGCTTC TTCCCGCTGT CTCTGAAGAA TCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTGAAC CTGGGACCTC AGGAGCCACC TTGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in GREB1L Cause Bilateral Kidney Agenesis in Humans and Mice.
American journal of human genetics101(5)803-814(2017 Nov)
De Tomasi L,David P,Humbert C,Silbermann F,Arrondel C,Tores F,Fouquet S,Desgrange A,Niel O,Bole-Feysot C,Nitschk? P,Roume J,Cordier MP,Pietrement C,Isidor B,Khau Van Kien P,Gonzales M,Saint-Frison MH,Martinovic J,Novo R,Piard J,Cabrol C,Verma IC,Puri R,Journel H,Aziza J,Gavard L,Said-Menthon MH,Heidet L,Saunier S,Jeanpierre C

Growth regulation by estrogen in breast cancer 1 (GREB1) is a novel progesterone-responsive gene required for human endometrial stromal decidualization.
Molecular human reproduction23(9)646-653(2017 Sep)
Camden AJ,Szwarc MM,Chadchan SB,DeMayo FJ,O'Malley BW,Lydon JP,Kommagani R

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

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Greb1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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