Robo2 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Robo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Robo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu79345 NM_001358494.1
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu52766 XM_006523046.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu52768 XM_006523048.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu52769 XM_006523049.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu74355 XM_011246131.3
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu79342 XM_017317015.2
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu79343 XM_017317016.2
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu79344 XM_017317017.1
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu79345 XM_017317018.1
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu79341 NM_001358493.1
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52755 XM_006523035.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52756 XM_006523036.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52757 XM_006523037.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52758 XM_030249140.1
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52758 XM_006523038.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52758 XM_030249139.1
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52759 XM_006523039.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52760 XM_006523040.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52761 XM_006523041.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52762 XM_006523042.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52763 XM_006523043.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52765 XM_006523045.4
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu79341 XM_017317013.1
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu86964 XM_030249141.1
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu15146 NM_175549.5
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $888.30
$1269.00
OMu52764 NM_001358490.1
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52767 NM_001358491.1
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu74356 NM_001358492.1
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu52764 XM_006523044.3
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52767 XM_006523047.3
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu74356 XM_011246132.2
Latest version!
Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OMu52758 XM_006523038.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52757 XM_006523037.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52760 XM_006523040.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52766 XM_006523046.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu74355 XM_011246131.2 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu52755 XM_006523035.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52761 XM_006523041.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52763 XM_006523043.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu79342 XM_017317015.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu52759 XM_006523039.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52768 XM_006523048.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu79343 XM_017317016.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu52769 XM_006523049.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu15146 NM_175549.4 Mus musculus roundabout guidance receptor 2 (Robo2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $888.30
$1269.00
OMu52765 XM_006523045.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52756 XM_006523036.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52762 XM_006523042.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu79345
    Clone ID Related Accession (Same CDS sequence) NM_001358494.1 , XM_017317018.1
    Accession Version NM_001358494.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform 6 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158234.2, AC112155.3, AC134452.4, AC122049.2 and AC162374.4. On Dec 1, 2017 this sequence version replaced XM_017317018.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.207525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC
    ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG
    GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT
    CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG
    TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT
    ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG
    AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC
    AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG
    ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA
    GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA
    GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT
    GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG
    ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG
    GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT
    GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT
    AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA
    AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC
    AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG
    ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT
    CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT
    AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT
    AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG
    AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT
    GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC
    AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG
    GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA
    TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT
    GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG
    CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA
    AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT
    CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT
    GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT
    ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG
    GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA
    CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG
    TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA
    GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA
    GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA
    GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG AAAAGCCTTT
    AGCTCCTCTC AAAGGCAGCG GCCCACCAGC CCATTTTCTA CGGACAGTAA CACCAGTGCT
    GCCCAGAATC AAAGCCAGAG GCCTCGGCCC ACGAAAAAAC ATAAGGGAGG ACGGATGGAC
    CCACAGCCAG TGTTACCTCA TCGGAGGGAA GGGATGCCAG ATGAGGAGTC CCTGGTGCCC
    TACAGCAAGC CCAGCTTCCC GTCTCCTGGC GGGCACAGCT CATCAGGAAC ATCTTCCTCT
    AAAGGGTCCA CTGGCCCTCG GAAGGCTGAC GTGCTGAGAG GCAGCCACCA GAGGAATGCC
    AATGACCTTC TCGACATTGG ATATGTGGGC TCAAATAGTC AAGGACAGTT TACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001345423.1
    CDS914..5053
    Translation

    Target ORF information:

    RefSeq Version NM_001358494.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001358494.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC
    ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG
    GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT
    CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG
    TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT
    ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG
    AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC
    AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG
    ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA
    GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA
    GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT
    GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG
    ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG
    GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT
    GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT
    AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA
    AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC
    AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG
    ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT
    CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT
    AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT
    AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG
    AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT
    GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC
    AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG
    GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA
    TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT
    GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG
    CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA
    AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT
    CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT
    GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT
    ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG
    GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA
    CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG
    TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA
    GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA
    GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA
    GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG AAAAGCCTTT
    AGCTCCTCTC AAAGGCAGCG GCCCACCAGC CCATTTTCTA CGGACAGTAA CACCAGTGCT
    GCCCAGAATC AAAGCCAGAG GCCTCGGCCC ACGAAAAAAC ATAAGGGAGG ACGGATGGAC
    CCACAGCCAG TGTTACCTCA TCGGAGGGAA GGGATGCCAG ATGAGGAGTC CCTGGTGCCC
    TACAGCAAGC CCAGCTTCCC GTCTCCTGGC GGGCACAGCT CATCAGGAAC ATCTTCCTCT
    AAAGGGTCCA CTGGCCCTCG GAAGGCTGAC GTGCTGAGAG GCAGCCACCA GAGGAATGCC
    AATGACCTTC TCGACATTGG ATATGTGGGC TCAAATAGTC AAGGACAGTT TACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52766
    Clone ID Related Accession (Same CDS sequence) XM_006523046.3 , XM_006523046.4
    Accession Version XM_006523046.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523046.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC
    CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC
    TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA
    GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG
    CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC
    CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC
    CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT
    CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT
    GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523109.1
    CDS9440..13927
    Translation

    Target ORF information:

    RefSeq Version XM_006523046.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523046.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC
    CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC
    TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA
    GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG
    CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC
    CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC
    CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT
    CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT
    GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52768
    Clone ID Related Accession (Same CDS sequence) XM_006523048.4 , XM_006523048.3
    Accession Version XM_006523048.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4335bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523048.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC
    AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG
    GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG
    GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA
    GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC
    CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA
    CAGTTTACAG AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523111.1
    CDS9440..13774
    Translation

    Target ORF information:

    RefSeq Version XM_006523048.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523048.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC
    AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG
    GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG
    GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA
    GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC
    CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA
    CAGTTTACAG AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52769
    Clone ID Related Accession (Same CDS sequence) XM_006523049.3 , XM_006523049.4
    Accession Version XM_006523049.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523049.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC
    CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC
    ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA
    GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA
    CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG
    AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT
    TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT
    TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT
    TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT
    ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT
    GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA
    ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG
    ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT
    ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC
    GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG
    GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA
    CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT
    GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC
    GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT
    GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT
    GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC
    TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC
    TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG
    CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC
    ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC
    GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT
    AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG
    CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT
    GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT
    GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT
    CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA
    CCTGATCAGA ACAAGGGGAA CAACGGTGGG AAAGGTGGAA AAAAGAAGAA AACTAAAAAT
    TCTTCGAAAG CGCAGAAAAA CAACGGATCC ACTTGGGCTA ATGTCCCTCT ACCTCCTCCT
    CCCGTCCAGC CCCTTCCTGG TACAGAGCTG GGCCACTATG CTGCGGAACA AGAAAATGGC
    TATGACAGTG ACAGCTGGTG CCCACCATTA CCGGTGCAAA CATACTTGCA TCAGGGTATG
    GAAGATGAGC TGGAAGAAGA CGAAGATCGG GTCCCAACAC CTCCTGTTCG CGGCGTGGCC
    TCTTCACCAG CTATCTCTTT TGGACAGCAG TCCACTGCCA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAAGCTCAC TTGGATGAGT TGACAAGGGC CTATCAGTTT
    GATATAGCAA AACAAACATG GCACATTCAA AGCAATACCC CACCTCCACA ACCCCCAGCT
    CCGCCATTAG GTTACGTGTC CGGAGCCTTG ATTTCTGATT TGGAGACAGA TGTTCCAGAT
    GAGGATGCTG ATGATGAAGA GGAACCATTA GAAATTCCCA GGCCCCTCAG AGCACTAGAC
    CAGACACCTG GATCCAGTAT GGACAATCTA GACAGCTCTG TCACAGGAAA AGCCTTTAGC
    TCCTCTCAAA GGCAGCGGCC CACCAGCCCA TTTTCTACGG ACAGTAACAC CAGTGCTGCC
    CAGAATCAAA GCCAGAGGCC TCGGCCCACG AAAAAACATA AGGGAGGACG GATGGACCCA
    CAGCCAGTGT TACCTCATCG GAGGGAAGGG ATGCCAGATG AGGAGTCCCT GGTGCCCTAC
    AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG CACAGCTCAT CAGGAACATC TTCCTCTAAA
    GGGTCCACTG GCCCTCGGAA GGCTGACGTG CTGAGAGGCA GCCACCAGAG GAATGCCAAT
    GACCTTCTCG ACATTGGATA TGTGGGCTCA AATAGTCAAG GACAGTTTAC AGGTGAATTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523112.1
    CDS9440..13642
    Translation

    Target ORF information:

    RefSeq Version XM_006523049.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523049.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC
    CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC
    ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA
    GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA
    CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG
    AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT
    TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT
    TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT
    TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT
    ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT
    GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA
    ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG
    ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT
    ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC
    GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG
    GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA
    CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT
    GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC
    GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT
    GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT
    GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC
    TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC
    TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG
    CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC
    ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC
    GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT
    AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG
    CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT
    GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT
    GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT
    CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA
    CCTGATCAGA ACAAGGGGAA CAACGGTGGG AAAGGTGGAA AAAAGAAGAA AACTAAAAAT
    TCTTCGAAAG CGCAGAAAAA CAACGGATCC ACTTGGGCTA ATGTCCCTCT ACCTCCTCCT
    CCCGTCCAGC CCCTTCCTGG TACAGAGCTG GGCCACTATG CTGCGGAACA AGAAAATGGC
    TATGACAGTG ACAGCTGGTG CCCACCATTA CCGGTGCAAA CATACTTGCA TCAGGGTATG
    GAAGATGAGC TGGAAGAAGA CGAAGATCGG GTCCCAACAC CTCCTGTTCG CGGCGTGGCC
    TCTTCACCAG CTATCTCTTT TGGACAGCAG TCCACTGCCA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAAGCTCAC TTGGATGAGT TGACAAGGGC CTATCAGTTT
    GATATAGCAA AACAAACATG GCACATTCAA AGCAATACCC CACCTCCACA ACCCCCAGCT
    CCGCCATTAG GTTACGTGTC CGGAGCCTTG ATTTCTGATT TGGAGACAGA TGTTCCAGAT
    GAGGATGCTG ATGATGAAGA GGAACCATTA GAAATTCCCA GGCCCCTCAG AGCACTAGAC
    CAGACACCTG GATCCAGTAT GGACAATCTA GACAGCTCTG TCACAGGAAA AGCCTTTAGC
    TCCTCTCAAA GGCAGCGGCC CACCAGCCCA TTTTCTACGG ACAGTAACAC CAGTGCTGCC
    CAGAATCAAA GCCAGAGGCC TCGGCCCACG AAAAAACATA AGGGAGGACG GATGGACCCA
    CAGCCAGTGT TACCTCATCG GAGGGAAGGG ATGCCAGATG AGGAGTCCCT GGTGCCCTAC
    AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG CACAGCTCAT CAGGAACATC TTCCTCTAAA
    GGGTCCACTG GCCCTCGGAA GGCTGACGTG CTGAGAGGCA GCCACCAGAG GAATGCCAAT
    GACCTTCTCG ACATTGGATA TGTGGGCTCA AATAGTCAAG GACAGTTTAC AGGTGAATTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74355
    Clone ID Related Accession (Same CDS sequence) XM_011246131.2 , XM_011246131.3
    Accession Version XM_011246131.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246131.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT
    TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA
    TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA
    TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA
    GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT
    AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA
    GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGAGGAG
    TCCCTGGTGC CCTACAGCAA GCCCAGCTTC CCGTCTCCTG GCGGGCACAG CTCATCAGGA
    ACATCTTCCT CTAAAGGGTC CACTGGCCCT CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC
    CAGAGGAATG CCAATGACCT TCTCGACATT GGATATGTGG GCTCAAATAG TCAAGGACAG
    TTTACAGGTG AATTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011244433.1
    CDS9440..13837
    Translation

    Target ORF information:

    RefSeq Version XM_011246131.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011246131.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT
    TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA
    TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA
    TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA
    GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT
    AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA
    GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGAGGAG
    TCCCTGGTGC CCTACAGCAA GCCCAGCTTC CCGTCTCCTG GCGGGCACAG CTCATCAGGA
    ACATCTTCCT CTAAAGGGTC CACTGGCCCT CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC
    CAGAGGAATG CCAATGACCT TCTCGACATT GGATATGTGG GCTCAAATAG TCAAGGACAG
    TTTACAGGTG AATTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79342
    Clone ID Related Accession (Same CDS sequence) XM_017317015.1 , XM_017317015.2
    Accession Version XM_017317015.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4341bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC
    AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG
    GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG
    GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA
    GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC
    CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA
    CAGTTTACAG GTGAATTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172504.1
    CDS337..4677
    Translation

    Target ORF information:

    RefSeq Version XM_017317015.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC
    AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG
    GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG
    GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA
    GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC
    CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA
    CAGTTTACAG GTGAATTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79342
    Clone ID Related Accession (Same CDS sequence) XM_017317015.1 , XM_017317015.2
    Accession Version XM_017317015.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4341bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC
    AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG
    GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG
    GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA
    GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC
    CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA
    CAGTTTACAG GTGAATTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172504.1
    CDS9440..13780
    Translation

    Target ORF information:

    RefSeq Version XM_017317015.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317015.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC
    AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG
    GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG
    GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA
    GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC
    CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA
    CAGTTTACAG GTGAATTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79343
    Clone ID Related Accession (Same CDS sequence) XM_017317016.2 , XM_017317016.1
    Accession Version XM_017317016.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC
    CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA
    TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG
    AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT
    ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172505.1
    CDS9440..13648
    Translation

    Target ORF information:

    RefSeq Version XM_017317016.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317016.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC
    CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA
    TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG
    AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT
    ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79343
    Clone ID Related Accession (Same CDS sequence) XM_017317016.2 , XM_017317016.1
    Accession Version XM_017317016.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC
    CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA
    TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG
    AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT
    ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172505.1
    CDS337..4545
    Translation

    Target ORF information:

    RefSeq Version XM_017317016.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC
    CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA
    TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG
    AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT
    ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79344
    Clone ID Related Accession (Same CDS sequence) XM_017317017.1
    Accession Version XM_017317017.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4185bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
    ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT
    CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA
    GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC
    AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG
    CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA
    GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT
    CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA
    GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC
    TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT
    GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA
    ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC
    ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT
    TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG
    CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT
    ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT
    GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA
    GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA
    CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT
    CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC
    CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA
    GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG
    CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC
    ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA
    GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT
    GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA
    GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC
    CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC
    TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC
    CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA
    GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA
    GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA
    GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC
    CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA
    CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA
    ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT
    TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA
    ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG
    TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT
    CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA
    AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA
    GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG
    GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT
    ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT
    ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC
    TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT
    GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC
    CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC
    CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG
    GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC
    AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA
    ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA
    AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC
    AAGGGGAACA ACGGTGGGAA AGGTGGAAAA AAGAAGAAAA CTAAAAATTC TTCGAAAGCG
    CAGAAAAACA ACGGATCCAC TTGGGCTAAT GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC
    CTTCCTGGTA CAGAGCTGGG CCACTATGCT GCGGAACAAG AAAATGGCTA TGACAGTGAC
    AGCTGGTGCC CACCATTACC GGTGCAAACA TACTTGCATC AGGGTATGGA AGATGAGCTG
    GAAGAAGACG AAGATCGGGT CCCAACACCT CCTGTTCGCG GCGTGGCCTC TTCACCAGCT
    ATCTCTTTTG GACAGCAGTC CACTGCCACT CTTACTCCAT CCCCACGGGA AGAGATGCAA
    CCCATGCTGC AAGCTCACTT GGATGAGTTG ACAAGGGCCT ATCAGTTTGA TATAGCAAAA
    CAAACATGGC ACATTCAAAG CAATACCCCA CCTCCACAAC CCCCAGCTCC GCCATTAGGT
    TACGTGTCCG GAGCCTTGAT TTCTGATTTG GAGACAGATG TTCCAGATGA GGATGCTGAT
    GATGAAGAGG AACCATTAGA AATTCCCAGG CCCCTCAGAG CACTAGACCA GACACCTGGA
    TCCAGTATGG ACAATCTAGA CAGCTCTGTC ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG
    CAGCGGCCCA CCAGCCCATT TTCTACGGAC AGTAACACCA GTGCTGCCCA GAATCAAAGC
    CAGAGGCCTC GGCCCACGAA AAAACATAAG GGAGGACGGA TGGACCCACA GCCAGTGTTA
    CCTCATCGGA GGGAAGGGAT GCCAGATGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC
    TTCCCGTCTC CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC
    CCTCGGAAGG CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC
    ATTGGATATG TGGGCTCAAA TAGTCAAGGA CAGTTTACAG AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172506.1
    CDS241..4425
    Translation

    Target ORF information:

    RefSeq Version XM_017317017.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317017.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
    ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT
    CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA
    GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC
    AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG
    CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA
    GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT
    CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA
    GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC
    TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT
    GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA
    ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC
    ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT
    TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG
    CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT
    ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT
    GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA
    GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA
    CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT
    CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC
    CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA
    GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG
    CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC
    ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA
    GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT
    GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA
    GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC
    CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC
    TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC
    CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA
    GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA
    GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA
    GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC
    CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA
    CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA
    ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT
    TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA
    ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG
    TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT
    CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA
    AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA
    GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG
    GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT
    ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT
    ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC
    TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT
    GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC
    CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC
    CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG
    GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC
    AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA
    ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA
    AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC
    AAGGGGAACA ACGGTGGGAA AGGTGGAAAA AAGAAGAAAA CTAAAAATTC TTCGAAAGCG
    CAGAAAAACA ACGGATCCAC TTGGGCTAAT GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC
    CTTCCTGGTA CAGAGCTGGG CCACTATGCT GCGGAACAAG AAAATGGCTA TGACAGTGAC
    AGCTGGTGCC CACCATTACC GGTGCAAACA TACTTGCATC AGGGTATGGA AGATGAGCTG
    GAAGAAGACG AAGATCGGGT CCCAACACCT CCTGTTCGCG GCGTGGCCTC TTCACCAGCT
    ATCTCTTTTG GACAGCAGTC CACTGCCACT CTTACTCCAT CCCCACGGGA AGAGATGCAA
    CCCATGCTGC AAGCTCACTT GGATGAGTTG ACAAGGGCCT ATCAGTTTGA TATAGCAAAA
    CAAACATGGC ACATTCAAAG CAATACCCCA CCTCCACAAC CCCCAGCTCC GCCATTAGGT
    TACGTGTCCG GAGCCTTGAT TTCTGATTTG GAGACAGATG TTCCAGATGA GGATGCTGAT
    GATGAAGAGG AACCATTAGA AATTCCCAGG CCCCTCAGAG CACTAGACCA GACACCTGGA
    TCCAGTATGG ACAATCTAGA CAGCTCTGTC ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG
    CAGCGGCCCA CCAGCCCATT TTCTACGGAC AGTAACACCA GTGCTGCCCA GAATCAAAGC
    CAGAGGCCTC GGCCCACGAA AAAACATAAG GGAGGACGGA TGGACCCACA GCCAGTGTTA
    CCTCATCGGA GGGAAGGGAT GCCAGATGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC
    TTCCCGTCTC CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC
    CCTCGGAAGG CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC
    ATTGGATATG TGGGCTCAAA TAGTCAAGGA CAGTTTACAG AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79345
    Clone ID Related Accession (Same CDS sequence) NM_001358494.1 , XM_017317018.1
    Accession Version XM_017317018.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product roundabout homolog 2 isoform X22
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC
    ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG
    GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT
    CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG
    TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT
    ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG
    AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC
    AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG
    ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA
    GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA
    GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT
    GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG
    ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG
    GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT
    GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT
    AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA
    AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC
    AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG
    ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT
    CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT
    AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT
    AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG
    AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT
    GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC
    AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG
    GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA
    TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT
    GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG
    CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA
    AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT
    CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT
    GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT
    ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG
    GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA
    CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG
    TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA
    GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA
    GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA
    GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG AAAAGCCTTT
    AGCTCCTCTC AAAGGCAGCG GCCCACCAGC CCATTTTCTA CGGACAGTAA CACCAGTGCT
    GCCCAGAATC AAAGCCAGAG GCCTCGGCCC ACGAAAAAAC ATAAGGGAGG ACGGATGGAC
    CCACAGCCAG TGTTACCTCA TCGGAGGGAA GGGATGCCAG ATGAGGAGTC CCTGGTGCCC
    TACAGCAAGC CCAGCTTCCC GTCTCCTGGC GGGCACAGCT CATCAGGAAC ATCTTCCTCT
    AAAGGGTCCA CTGGCCCTCG GAAGGCTGAC GTGCTGAGAG GCAGCCACCA GAGGAATGCC
    AATGACCTTC TCGACATTGG ATATGTGGGC TCAAATAGTC AAGGACAGTT TACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172507.1
    CDS874..5013
    Translation

    Target ORF information:

    RefSeq Version XM_017317018.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317018.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC
    ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG
    GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT
    CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG
    TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT
    ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG
    AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC
    AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG
    ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA
    GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA
    GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT
    GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG
    ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG
    GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT
    GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT
    AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA
    AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC
    AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG
    ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT
    CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT
    AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT
    AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG
    AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT
    GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC
    AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG
    GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA
    TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT
    GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG
    CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA
    AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT
    CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT
    GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT
    ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG
    GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA
    CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG
    TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA
    GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA
    GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA
    GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG AAAAGCCTTT
    AGCTCCTCTC AAAGGCAGCG GCCCACCAGC CCATTTTCTA CGGACAGTAA CACCAGTGCT
    GCCCAGAATC AAAGCCAGAG GCCTCGGCCC ACGAAAAAAC ATAAGGGAGG ACGGATGGAC
    CCACAGCCAG TGTTACCTCA TCGGAGGGAA GGGATGCCAG ATGAGGAGTC CCTGGTGCCC
    TACAGCAAGC CCAGCTTCCC GTCTCCTGGC GGGCACAGCT CATCAGGAAC ATCTTCCTCT
    AAAGGGTCCA CTGGCCCTCG GAAGGCTGAC GTGCTGAGAG GCAGCCACCA GAGGAATGCC
    AATGACCTTC TCGACATTGG ATATGTGGGC TCAAATAGTC AAGGACAGTT TACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79341
    Clone ID Related Accession (Same CDS sequence) XM_017317013.1 , NM_001358493.1
    Accession Version NM_001358493.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4608bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115120.4, AC158234.2, AC112155.3, AC134452.4, AC122049.2 and AC162374.4. On Dec 1, 2017 this sequence version replaced XM_017317013.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC
    AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG
    GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT
    CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC
    CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA
    CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA
    GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG
    AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC
    AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT
    GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC
    GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001345422.1
    CDS353..4960
    Translation

    Target ORF information:

    RefSeq Version NM_001358493.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001358493.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC
    AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG
    GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT
    CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC
    CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA
    CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA
    GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG
    AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC
    AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT
    GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC
    GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52755
    Clone ID Related Accession (Same CDS sequence) XM_006523035.4 , XM_006523035.3
    Accession Version XM_006523035.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523035.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT
    CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA
    AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG
    AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG
    GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT
    AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT
    GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG
    AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA
    CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT
    CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT
    CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG
    CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC
    ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT
    CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGGTGA ATTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523098.1
    CDS9440..14236
    Translation

    Target ORF information:

    RefSeq Version XM_006523035.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523035.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT
    CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA
    AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG
    AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG
    GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT
    AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT
    GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG
    AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA
    CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT
    CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT
    CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG
    CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC
    ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT
    CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGGTGA ATTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52756
    Clone ID Related Accession (Same CDS sequence) XM_006523036.3 , XM_006523036.4
    Accession Version XM_006523036.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523036.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT
    CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA
    AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG
    AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG
    GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT
    AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT
    GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG
    AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA
    CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT
    CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT
    CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG
    CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC
    ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT
    CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523099.1
    CDS9440..14230
    Translation

    Target ORF information:

    RefSeq Version XM_006523036.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523036.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT
    CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA
    AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG
    AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG
    GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT
    AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT
    GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG
    AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA
    CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT
    CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT
    CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG
    CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC
    ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT
    CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52757
    Clone ID Related Accession (Same CDS sequence) XM_006523037.4 , XM_006523037.3
    Accession Version XM_006523037.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523037.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC
    CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC
    ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA
    GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA
    CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG
    AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT
    TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT
    TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT
    TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT
    ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT
    GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA
    ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG
    ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT
    ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC
    GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG
    GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA
    CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT
    GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC
    GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT
    GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT
    GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC
    TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC
    TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG
    CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC
    ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC
    GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT
    AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG
    CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT
    GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT
    GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT
    CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA
    CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT
    CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA
    AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG
    GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC
    GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC
    CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT
    CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA
    CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT
    GTAGGTAGCT CCTCAGATGG CTCCATCTTT GCCAGCGGCA GCTTTGCAAA AGCATTGGTG
    GCAGCAGCAG ACAAAGCTGG CTTTAGGCTG GATGGAACCA GTCTTACGAG AACAGGAAAA
    GCCTTTAGCT CCTCTCAAAG GCAGCGGCCC ACCAGCCCAT TTTCTACGGA CAGTAACACC
    AGTGCTGCCC AGAATCAAAG CCAGAGGCCT CGGCCCACGA AAAAACATAA GGGAGGACGG
    ATGGACCCAC AGCCAGTGTT ACCTCATCGG AGGGAAGGGA TGCCAGATGA TCTTCCCCCA
    CCACCAGATC CCCCACCAGG TCAGGGTTTA AGGCAGCAGA TAGGCCTGAG CCAACACTCT
    GGTAACGTGG AAAACTCAAC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACAAGCAGCC
    AACTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA AAACTGTTCT AGAGTGGCAG
    CGTCAAACCC AGGACTGGAT AAACTCTACA GAACGCCAAG AAGAAACTCG GAAAGCCCCA
    CACAAACAAG GGGTTGGATC AGAGGAGTCC CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG
    TCTCCTGGCG GGCACAGCTC ATCAGGAACA TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG
    AAGGCTGACG TGCTGAGAGG CAGCCACCAG AGGAATGCCA ATGACCTTCT CGACATTGGA
    TATGTGGGCT CAAATAGTCA AGGACAGTTT ACAGGTGAAT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523100.1
    CDS9440..14224
    Translation

    Target ORF information:

    RefSeq Version XM_006523037.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523037.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC
    CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC
    ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA
    GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA
    CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG
    AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT
    TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT
    TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT
    TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT
    ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT
    GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA
    ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG
    ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT
    ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC
    GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG
    GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA
    CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT
    GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC
    GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT
    GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT
    GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC
    TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC
    TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG
    CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC
    ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC
    GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT
    AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG
    CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT
    GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT
    GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT
    CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA
    CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT
    CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA
    AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG
    GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC
    GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC
    CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT
    CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA
    CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT
    GTAGGTAGCT CCTCAGATGG CTCCATCTTT GCCAGCGGCA GCTTTGCAAA AGCATTGGTG
    GCAGCAGCAG ACAAAGCTGG CTTTAGGCTG GATGGAACCA GTCTTACGAG AACAGGAAAA
    GCCTTTAGCT CCTCTCAAAG GCAGCGGCCC ACCAGCCCAT TTTCTACGGA CAGTAACACC
    AGTGCTGCCC AGAATCAAAG CCAGAGGCCT CGGCCCACGA AAAAACATAA GGGAGGACGG
    ATGGACCCAC AGCCAGTGTT ACCTCATCGG AGGGAAGGGA TGCCAGATGA TCTTCCCCCA
    CCACCAGATC CCCCACCAGG TCAGGGTTTA AGGCAGCAGA TAGGCCTGAG CCAACACTCT
    GGTAACGTGG AAAACTCAAC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACAAGCAGCC
    AACTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA AAACTGTTCT AGAGTGGCAG
    CGTCAAACCC AGGACTGGAT AAACTCTACA GAACGCCAAG AAGAAACTCG GAAAGCCCCA
    CACAAACAAG GGGTTGGATC AGAGGAGTCC CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG
    TCTCCTGGCG GGCACAGCTC ATCAGGAACA TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG
    AAGGCTGACG TGCTGAGAGG CAGCCACCAG AGGAATGCCA ATGACCTTCT CGACATTGGA
    TATGTGGGCT CAAATAGTCA AGGACAGTTT ACAGGTGAAT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52758
    Clone ID Related Accession (Same CDS sequence) XM_006523038.3 , XM_006523038.4 , XM_030249140.1 , XM_030249139.1
    Accession Version XM_006523038.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523038.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
    ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT
    CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA
    GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC
    AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG
    CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA
    GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT
    CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA
    GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC
    TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT
    GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA
    ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC
    ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT
    TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG
    CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT
    ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT
    GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA
    GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA
    CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT
    CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC
    CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA
    GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG
    CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC
    ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA
    GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT
    GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA
    GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC
    CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC
    TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC
    CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA
    GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA
    GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA
    GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC
    CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA
    CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA
    ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT
    TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA
    ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG
    TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT
    CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA
    AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA
    GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG
    GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT
    ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT
    ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC
    TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT
    GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC
    CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC
    CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG
    GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC
    AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA
    ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA
    AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC
    AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT
    AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA
    AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG
    AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC
    CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC
    AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT
    GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA
    CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA
    TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT
    GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA
    CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG
    GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC
    TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC
    AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC
    TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG
    AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG
    CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC
    CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA
    AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC
    ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG
    GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG
    GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG
    CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG
    CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA
    AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523101.1
    CDS386..5158
    Translation

    Target ORF information:

    RefSeq Version XM_006523038.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523038.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
    ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT
    CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA
    GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC
    AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG
    CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA
    GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT
    CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA
    GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC
    TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT
    GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA
    ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC
    ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT
    TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG
    CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT
    ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT
    GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA
    GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA
    CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT
    CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC
    CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA
    GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG
    CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC
    ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA
    GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT
    GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA
    GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC
    CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC
    TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC
    CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA
    GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA
    GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA
    GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC
    CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA
    CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA
    ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT
    TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA
    ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG
    TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT
    CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA
    AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA
    GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG
    GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT
    ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT
    ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC
    TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT
    GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC
    CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC
    CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG
    GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC
    AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA
    ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA
    AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC
    AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT
    AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA
    AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG
    AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC
    CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC
    AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT
    GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA
    CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA
    TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT
    GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA
    CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG
    GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC
    TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC
    AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC
    TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG
    AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG
    CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC
    CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA
    AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC
    ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG
    GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG
    GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG
    CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG
    CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA
    AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52758
    Clone ID Related Accession (Same CDS sequence) XM_006523038.3 , XM_006523038.4 , XM_030249140.1 , XM_030249139.1
    Accession Version XM_030249140.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
    ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT
    CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA
    GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC
    AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG
    CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA
    GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT
    CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA
    GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC
    TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT
    GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA
    ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC
    ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT
    TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG
    CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT
    ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT
    GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA
    GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA
    CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT
    CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC
    CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA
    GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG
    CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC
    ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA
    GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT
    GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA
    GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC
    CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC
    TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC
    CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA
    GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA
    GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA
    GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC
    CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA
    CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA
    ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT
    TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA
    ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG
    TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT
    CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA
    AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA
    GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG
    GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT
    ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT
    ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC
    TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT
    GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC
    CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC
    CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG
    GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC
    AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA
    ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA
    AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC
    AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT
    AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA
    AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG
    AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC
    CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC
    AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT
    GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA
    CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA
    TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT
    GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA
    CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG
    GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC
    TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC
    AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC
    TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG
    AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG
    CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC
    CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA
    AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC
    ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG
    GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG
    GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG
    CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG
    CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA
    AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105000.1
    CDS112..4884
    Translation

    Target ORF information:

    RefSeq Version XM_030249140.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249140.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
    ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT
    CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA
    GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC
    AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG
    CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA
    GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT
    CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA
    GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC
    TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT
    GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA
    ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC
    ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT
    TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG
    CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT
    ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT
    GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA
    GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA
    CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT
    CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC
    CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA
    GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG
    CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC
    ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA
    GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT
    GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA
    GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC
    CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC
    TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC
    CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA
    GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA
    GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA
    GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC
    CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA
    CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA
    ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT
    TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA
    ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG
    TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT
    CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA
    AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA
    GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG
    GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT
    ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT
    ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC
    TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT
    GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC
    CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC
    CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG
    GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC
    AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA
    ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA
    AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC
    AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT
    AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA
    AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG
    AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC
    CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC
    AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT
    GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA
    CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA
    TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT
    GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA
    CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG
    GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC
    TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC
    AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC
    TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG
    AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG
    CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC
    CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA
    AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC
    ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG
    GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG
    GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG
    CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG
    CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA
    AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52758
    Clone ID Related Accession (Same CDS sequence) XM_006523038.3 , XM_006523038.4 , XM_030249140.1 , XM_030249139.1
    Accession Version XM_030249139.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
    ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT
    CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA
    GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC
    AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG
    CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA
    GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT
    CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA
    GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC
    TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT
    GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA
    ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC
    ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT
    TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG
    CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT
    ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT
    GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA
    GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA
    CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT
    CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC
    CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA
    GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG
    CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC
    ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA
    GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT
    GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA
    GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC
    CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC
    TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC
    CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA
    GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA
    GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA
    GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC
    CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA
    CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA
    ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT
    TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA
    ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG
    TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT
    CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA
    AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA
    GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG
    GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT
    ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT
    ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC
    TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT
    GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC
    CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC
    CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG
    GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC
    AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA
    ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA
    AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC
    AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT
    AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA
    AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG
    AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC
    CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC
    AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT
    GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA
    CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA
    TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT
    GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA
    CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG
    GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC
    TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC
    AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC
    TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG
    AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG
    CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC
    CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA
    AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC
    ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG
    GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG
    GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG
    CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG
    CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA
    AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030104999.1
    CDS233..5005
    Translation

    Target ORF information:

    RefSeq Version XM_030249139.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249139.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
    ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT
    CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA
    GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC
    AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG
    CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA
    GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT
    CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA
    GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC
    TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT
    GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA
    ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC
    ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT
    TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG
    CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT
    ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT
    GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA
    GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA
    CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT
    CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC
    CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA
    GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG
    CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC
    ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA
    GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT
    GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA
    GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC
    CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC
    TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC
    CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA
    GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA
    GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA
    GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC
    CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA
    CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA
    ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT
    TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA
    ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG
    TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT
    CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA
    AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA
    GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG
    GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT
    ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT
    ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC
    TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT
    GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC
    CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC
    CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG
    GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC
    AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA
    ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA
    AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC
    AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT
    AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA
    AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG
    AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC
    CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC
    AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT
    GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA
    CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA
    TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT
    GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA
    CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG
    GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC
    TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC
    AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC
    TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG
    AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG
    CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC
    CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA
    AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC
    ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG
    GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG
    GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG
    CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG
    CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA
    AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52759
    Clone ID Related Accession (Same CDS sequence) XM_006523039.4 , XM_006523039.3
    Accession Version XM_006523039.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4728bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523039.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC
    ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG
    GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT
    CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG
    TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT
    ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG
    AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC
    AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG
    ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA
    GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA
    GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT
    GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG
    ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG
    GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT
    GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT
    AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA
    AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC
    AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG
    ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT
    CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT
    AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT
    AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG
    AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT
    GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC
    AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG
    GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA
    TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT
    GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG
    CTACCTGATC AGAACAAGGG GAACAACGCC TTACTTTACA TCCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    ACTAAAAATT CTTCGAAAGC GCAGAAAAAC AACGGATCCA CTTGGGCTAA TGTCCCTCTA
    CCTCCTCCTC CCGTCCAGCC CCTTCCTGGT ACAGAGCTGG GCCACTATGC TGCGGAACAA
    GAAAATGGCT ATGACAGTGA CAGCTGGTGC CCACCATTAC CGGTGCAAAC ATACTTGCAT
    CAGGGTATGG AAGATGAGCT GGAAGAAGAC GAAGATCGGG TCCCAACACC TCCTGTTCGC
    GGCGTGGCCT CTTCACCAGC TATCTCTTTT GGACAGCAGT CCACTGCCAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACT TGGATGAGTT GACAAGGGCC
    TATCAGTTTG ATATAGCAAA ACAAACATGG CACATTCAAA GCAATACCCC ACCTCCACAA
    CCCCCAGCTC CGCCATTAGG TTACGTGTCC GGAGCCTTGA TTTCTGATTT GGAGACAGAT
    GTTCCAGATG AGGATGCTGA TGATGAAGAG GAACCATTAG AAATTCCCAG GCCCCTCAGA
    GCACTAGACC AGACACCTGG ATCCAGTATG GACAATCTAG ACAGCTCTGT CACAGGTTCA
    ATGGTAAATG GATGGGGCTC TGCATCTGAT GAGGATCGTA ACTTTTCTAG TCATAGATCT
    AGTGTAGGTA GCTCCTCAGA TGGCTCCATC TTTGCCAGCG GCAGCTTTGC AAAAGCATTG
    GTGGCAGCAG CAGACAAAGC TGGCTTTAGG CTGGATGGAA CCAGTCTTAC GAGAACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC
    CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC
    TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA
    GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG
    CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC
    CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC
    CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT
    CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT
    GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523102.1
    CDS2416..7143
    Translation

    Target ORF information:

    RefSeq Version XM_006523039.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523039.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC
    ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG
    GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT
    CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG
    TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT
    ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG
    AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC
    AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG
    ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA
    GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA
    GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT
    GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG
    ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG
    GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT
    GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT
    AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA
    AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC
    AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG
    ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT
    CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT
    AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT
    AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG
    AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT
    GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC
    AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG
    GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA
    TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT
    GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG
    CTACCTGATC AGAACAAGGG GAACAACGCC TTACTTTACA TCCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    ACTAAAAATT CTTCGAAAGC GCAGAAAAAC AACGGATCCA CTTGGGCTAA TGTCCCTCTA
    CCTCCTCCTC CCGTCCAGCC CCTTCCTGGT ACAGAGCTGG GCCACTATGC TGCGGAACAA
    GAAAATGGCT ATGACAGTGA CAGCTGGTGC CCACCATTAC CGGTGCAAAC ATACTTGCAT
    CAGGGTATGG AAGATGAGCT GGAAGAAGAC GAAGATCGGG TCCCAACACC TCCTGTTCGC
    GGCGTGGCCT CTTCACCAGC TATCTCTTTT GGACAGCAGT CCACTGCCAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACT TGGATGAGTT GACAAGGGCC
    TATCAGTTTG ATATAGCAAA ACAAACATGG CACATTCAAA GCAATACCCC ACCTCCACAA
    CCCCCAGCTC CGCCATTAGG TTACGTGTCC GGAGCCTTGA TTTCTGATTT GGAGACAGAT
    GTTCCAGATG AGGATGCTGA TGATGAAGAG GAACCATTAG AAATTCCCAG GCCCCTCAGA
    GCACTAGACC AGACACCTGG ATCCAGTATG GACAATCTAG ACAGCTCTGT CACAGGTTCA
    ATGGTAAATG GATGGGGCTC TGCATCTGAT GAGGATCGTA ACTTTTCTAG TCATAGATCT
    AGTGTAGGTA GCTCCTCAGA TGGCTCCATC TTTGCCAGCG GCAGCTTTGC AAAAGCATTG
    GTGGCAGCAG CAGACAAAGC TGGCTTTAGG CTGGATGGAA CCAGTCTTAC GAGAACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC
    CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC
    TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA
    GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG
    CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC
    CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC
    CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT
    CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT
    GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52760
    Clone ID Related Accession (Same CDS sequence) XM_006523040.4 , XM_006523040.3
    Accession Version XM_006523040.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523040.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT
    TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA
    TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA
    TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA
    GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT
    AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA
    GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGATCTT
    CCCCCACCAC CAGATCCCCC ACCAGGTCAG GGTTTAAGGC AGCAGATAGG CCTGAGCCAA
    CACTCTGGTA ACGTGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAA
    GCAGCCAACT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAAAAC TGTTCTAGAG
    TGGCAGCGTC AAACCCAGGA CTGGATAAAC TCTACAGAAC GCCAAGAAGA AACTCGGAAA
    GCCCCACACA AACAAGGGGT TGGATCAGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC
    TTCCCGTCTC CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC
    CCTCGGAAGG CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC
    ATTGGATATG TGGGCTCAAA TAGTCAAGGA CAGTTTACAG GTGAATTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523103.1
    CDS9440..14110
    Translation

    Target ORF information:

    RefSeq Version XM_006523040.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523040.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT
    TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA
    TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA
    TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA
    GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT
    AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA
    GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGATCTT
    CCCCCACCAC CAGATCCCCC ACCAGGTCAG GGTTTAAGGC AGCAGATAGG CCTGAGCCAA
    CACTCTGGTA ACGTGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAA
    GCAGCCAACT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAAAAC TGTTCTAGAG
    TGGCAGCGTC AAACCCAGGA CTGGATAAAC TCTACAGAAC GCCAAGAAGA AACTCGGAAA
    GCCCCACACA AACAAGGGGT TGGATCAGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC
    TTCCCGTCTC CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC
    CCTCGGAAGG CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC
    ATTGGATATG TGGGCTCAAA TAGTCAAGGA CAGTTTACAG GTGAATTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52761
    Clone ID Related Accession (Same CDS sequence) XM_006523041.3 , XM_006523041.4
    Accession Version XM_006523041.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523041.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC
    AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG
    GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT
    CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC
    CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA
    CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA
    GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG
    AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC
    AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT
    GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC
    GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGGTGAATT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523104.1
    CDS9440..14053
    Translation

    Target ORF information:

    RefSeq Version XM_006523041.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523041.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC
    AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG
    GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT
    CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC
    CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA
    CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA
    GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG
    AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC
    AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT
    GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC
    GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGGTGAATT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52762
    Clone ID Related Accession (Same CDS sequence) XM_006523042.3 , XM_006523042.4
    Accession Version XM_006523042.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523042.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC
    CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC
    ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA
    GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA
    CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG
    AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT
    TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT
    TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT
    TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT
    ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT
    GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA
    ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG
    ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT
    ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC
    GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG
    GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA
    CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT
    GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC
    GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT
    GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT
    GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC
    TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC
    TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG
    CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC
    ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC
    GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT
    AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG
    CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT
    GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT
    GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT
    CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA
    CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT
    CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA
    AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG
    GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC
    GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC
    CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT
    CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA
    CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGAAAAGCC
    TTTAGCTCCT CTCAAAGGCA GCGGCCCACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCTGCCCAGA ATCAAAGCCA GAGGCCTCGG CCCACGAAAA AACATAAGGG AGGACGGATG
    GACCCACAGC CAGTGTTACC TCATCGGAGG GAAGGGATGC CAGATGATCT TCCCCCACCA
    CCAGATCCCC CACCAGGTCA GGGTTTAAGG CAGCAGATAG GCCTGAGCCA ACACTCTGGT
    AACGTGGAAA ACTCAACAGA GAGAAAAGGA AGCTCTCTAG AGAGACAACA AGCAGCCAAC
    TTAGAAGACA CAAAGAGCTC ATTGGATTGT CCAGCTAAAA CTGTTCTAGA GTGGCAGCGT
    CAAACCCAGG ACTGGATAAA CTCTACAGAA CGCCAAGAAG AAACTCGGAA AGCCCCACAC
    AAACAAGGGG TTGGATCAGA GGAGTCCCTG GTGCCCTACA GCAAGCCCAG CTTCCCGTCT
    CCTGGCGGGC ACAGCTCATC AGGAACATCT TCCTCTAAAG GGTCCACTGG CCCTCGGAAG
    GCTGACGTGC TGAGAGGCAG CCACCAGAGG AATGCCAATG ACCTTCTCGA CATTGGATAT
    GTGGGCTCAA ATAGTCAAGG ACAGTTTACA GGTGAATTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523105.1
    CDS9440..14041
    Translation

    Target ORF information:

    RefSeq Version XM_006523042.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523042.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC
    CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC
    ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA
    GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA
    CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG
    AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT
    TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT
    TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT
    TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT
    ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT
    GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA
    ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG
    ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT
    ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC
    GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG
    GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA
    CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT
    GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC
    GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT
    GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT
    GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC
    TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC
    TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG
    CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC
    ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC
    GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT
    AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG
    CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT
    GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT
    GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT
    CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA
    CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT
    CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA
    AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG
    GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC
    GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC
    CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT
    CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA
    CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGAAAAGCC
    TTTAGCTCCT CTCAAAGGCA GCGGCCCACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCTGCCCAGA ATCAAAGCCA GAGGCCTCGG CCCACGAAAA AACATAAGGG AGGACGGATG
    GACCCACAGC CAGTGTTACC TCATCGGAGG GAAGGGATGC CAGATGATCT TCCCCCACCA
    CCAGATCCCC CACCAGGTCA GGGTTTAAGG CAGCAGATAG GCCTGAGCCA ACACTCTGGT
    AACGTGGAAA ACTCAACAGA GAGAAAAGGA AGCTCTCTAG AGAGACAACA AGCAGCCAAC
    TTAGAAGACA CAAAGAGCTC ATTGGATTGT CCAGCTAAAA CTGTTCTAGA GTGGCAGCGT
    CAAACCCAGG ACTGGATAAA CTCTACAGAA CGCCAAGAAG AAACTCGGAA AGCCCCACAC
    AAACAAGGGG TTGGATCAGA GGAGTCCCTG GTGCCCTACA GCAAGCCCAG CTTCCCGTCT
    CCTGGCGGGC ACAGCTCATC AGGAACATCT TCCTCTAAAG GGTCCACTGG CCCTCGGAAG
    GCTGACGTGC TGAGAGGCAG CCACCAGAGG AATGCCAATG ACCTTCTCGA CATTGGATAT
    GTGGGCTCAA ATAGTCAAGG ACAGTTTACA GGTGAATTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52763
    Clone ID Related Accession (Same CDS sequence) XM_006523043.3 , XM_006523043.4
    Accession Version XM_006523043.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523043.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC
    ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG
    GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT
    CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG
    TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT
    ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG
    AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC
    AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG
    ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA
    GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA
    GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT
    GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG
    ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG
    GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT
    GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT
    AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA
    AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC
    AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG
    ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT
    CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT
    AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT
    AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG
    AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT
    GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC
    AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG
    GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA
    TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT
    GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG
    CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA
    AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT
    CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT
    GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT
    ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG
    GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA
    CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG
    TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA
    GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA
    GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA
    GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG TTCAATGGTA
    AATGGATGGG GCTCTGCATC TGATGAGGAT CGTAACTTTT CTAGTCATAG ATCTAGTGTA
    GGTAGCTCCT CAGATGGCTC CATCTTTGCC AGCGGCAGCT TTGCAAAAGC ATTGGTGGCA
    GCAGCAGACA AAGCTGGCTT TAGGCTGGAT GGAACCAGTC TTACGAGAAC AGGAAAAGCC
    TTTAGCTCCT CTCAAAGGCA GCGGCCCACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCTGCCCAGA ATCAAAGCCA GAGGCCTCGG CCCACGAAAA AACATAAGGG AGGACGGATG
    GACCCACAGC CAGTGTTACC TCATCGGAGG GAAGGGATGC CAGATGATCT TCCCCCACCA
    CCAGATCCCC CACCAGGTCA GGGTTTAAGG CAGCAGATAG GCCTGAGCCA ACACTCTGGT
    AACGTGGAAA ACTCAACAGA GAGAAAAGGA AGCTCTCTAG AGAGACAACA AGCAGCCAAC
    TTAGAAGACA CAAAGAGCTC ATTGGATTGT CCAGCTAAAA CTGTTCTAGA GTGGCAGCGT
    CAAACCCAGG ACTGGATAAA CTCTACAGAA CGCCAAGAAG AAACTCGGAA AGCCCCACAC
    AAACAAGGGG TTGGATCAGA GGAGTCCCTG GTGCCCTACA GCAAGCCCAG CTTCCCGTCT
    CCTGGCGGGC ACAGCTCATC AGGAACATCT TCCTCTAAAG GGTCCACTGG CCCTCGGAAG
    GCTGACGTGC TGAGAGGCAG CCACCAGAGG AATGCCAATG ACCTTCTCGA CATTGGATAT
    GTGGGCTCAA ATAGTCAAGG ACAGTTTACA GGTGAATTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523106.1
    CDS2416..7017
    Translation

    Target ORF information:

    RefSeq Version XM_006523043.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523043.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC
    ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG
    GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT
    CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG
    TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT
    ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG
    AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC
    AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG
    ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA
    GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA
    GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT
    GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG
    ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG
    GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT
    GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT
    AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA
    AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC
    AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG
    ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT
    CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT
    AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT
    AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG
    AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT
    GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC
    AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG
    GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA
    TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT
    GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG
    CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA
    AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT
    CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT
    GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT
    ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG
    GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA
    CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG
    TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA
    GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA
    GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA
    GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG TTCAATGGTA
    AATGGATGGG GCTCTGCATC TGATGAGGAT CGTAACTTTT CTAGTCATAG ATCTAGTGTA
    GGTAGCTCCT CAGATGGCTC CATCTTTGCC AGCGGCAGCT TTGCAAAAGC ATTGGTGGCA
    GCAGCAGACA AAGCTGGCTT TAGGCTGGAT GGAACCAGTC TTACGAGAAC AGGAAAAGCC
    TTTAGCTCCT CTCAAAGGCA GCGGCCCACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCTGCCCAGA ATCAAAGCCA GAGGCCTCGG CCCACGAAAA AACATAAGGG AGGACGGATG
    GACCCACAGC CAGTGTTACC TCATCGGAGG GAAGGGATGC CAGATGATCT TCCCCCACCA
    CCAGATCCCC CACCAGGTCA GGGTTTAAGG CAGCAGATAG GCCTGAGCCA ACACTCTGGT
    AACGTGGAAA ACTCAACAGA GAGAAAAGGA AGCTCTCTAG AGAGACAACA AGCAGCCAAC
    TTAGAAGACA CAAAGAGCTC ATTGGATTGT CCAGCTAAAA CTGTTCTAGA GTGGCAGCGT
    CAAACCCAGG ACTGGATAAA CTCTACAGAA CGCCAAGAAG AAACTCGGAA AGCCCCACAC
    AAACAAGGGG TTGGATCAGA GGAGTCCCTG GTGCCCTACA GCAAGCCCAG CTTCCCGTCT
    CCTGGCGGGC ACAGCTCATC AGGAACATCT TCCTCTAAAG GGTCCACTGG CCCTCGGAAG
    GCTGACGTGC TGAGAGGCAG CCACCAGAGG AATGCCAATG ACCTTCTCGA CATTGGATAT
    GTGGGCTCAA ATAGTCAAGG ACAGTTTACA GGTGAATTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52765
    Clone ID Related Accession (Same CDS sequence) XM_006523045.4 , XM_006523045.3
    Accession Version XM_006523045.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523045.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT
    CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA
    AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG
    AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG
    GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT
    AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT
    GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC AGCTTCCCGT CTCCTGGCGG GCACAGCTCA
    TCAGGAACAT CTTCCTCTAA AGGGTCCACT GGCCCTCGGA AGGCTGACGT GCTGAGAGGC
    AGCCACCAGA GGAATGCCAA TGACCTTCTC GACATTGGAT ATGTGGGCTC AAATAGTCAA
    GGACAGTTTA CAGGTGAATT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523108.1
    CDS9440..13963
    Translation

    Target ORF information:

    RefSeq Version XM_006523045.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523045.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT
    CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA
    AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG
    AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG
    GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT
    AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT
    GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC AGCTTCCCGT CTCCTGGCGG GCACAGCTCA
    TCAGGAACAT CTTCCTCTAA AGGGTCCACT GGCCCTCGGA AGGCTGACGT GCTGAGAGGC
    AGCCACCAGA GGAATGCCAA TGACCTTCTC GACATTGGAT ATGTGGGCTC AAATAGTCAA
    GGACAGTTTA CAGGTGAATT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79341
    Clone ID Related Accession (Same CDS sequence) XM_017317013.1 , NM_001358493.1
    Accession Version XM_017317013.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4608bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product roundabout homolog 2 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC
    AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG
    GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT
    CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC
    CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA
    CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA
    GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG
    AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC
    AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT
    GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC
    GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172502.1
    CDS365..4972
    Translation

    Target ORF information:

    RefSeq Version XM_017317013.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317013.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC
    AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG
    GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT
    CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC
    CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA
    CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA
    GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG
    AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC
    AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT
    GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC
    GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu86964
    Clone ID Related Accession (Same CDS sequence) XM_030249141.1
    Accession Version XM_030249141.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT
    CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA
    AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG
    AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG
    GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT
    AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT
    GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC AGCTTCCCGT CTCCTGGCGG GCACAGCTCA
    TCAGGAACAT CTTCCTCTAA AGGGTCCACT GGCCCTCGGA AGGCTGACGT GCTGAGAGGC
    AGCCACCAGA GGAATGCCAA TGACCTTCTC GACATTGGAT ATGTGGGCTC AAATAGTCAA
    GGACAGTTTA CAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105001.1
    CDS9440..13957
    Translation

    Target ORF information:

    RefSeq Version XM_030249141.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249141.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT
    CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA
    AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG
    AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG
    GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT
    AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT
    GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC AGCTTCCCGT CTCCTGGCGG GCACAGCTCA
    TCAGGAACAT CTTCCTCTAA AGGGTCCACT GGCCCTCGGA AGGCTGACGT GCTGAGAGGC
    AGCCACCAGA GGAATGCCAA TGACCTTCTC GACATTGGAT ATGTGGGCTC AAATAGTCAA
    GGACAGTTTA CAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15146
    Clone ID Related Accession (Same CDS sequence) NM_175549.4 , NM_175549.5
    Accession Version NM_175549.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-27
    Organism Mus musculus(House mouse)
    Product roundabout homolog 2 precursor
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ533876.1. On Jul 28, 2006 this sequence version replaced NM_175549.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ533876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGCTCCTC CACAGTTTGT GGTTAGGCCA
    AGAGATCAGA TCGTTGCTCA AGGCCGAACA GTGACATTCC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCGAATCAA
    CCTCAGCAGC CCAACAGCCG ATGTTCAGTG TCGCCCACGG GGGACCTCAC CATCACCAAC
    ATCCAGCGTT CAGATGCGGG TTACTACATC TGCCAGGCCC TAACCGTGGC AGGAAGCATT
    TTAGCTAAAG CACAGTTGGA AGTTACTGAC GTTTTGACAG ATAGACCTCC ACCCATAATC
    TTGCAAGGAC CAATAAACCA AACACTTGCA GTAGACGGTA CAGCATTGTT GAAGTGTAAA
    GCCACTGGTG AGCCTCTGCC TGTAATTAGC TGGCTAAAGG AGGGCTTTAC TTTTCTGGGG
    AGAGATCCAA GAGCCACGAT CCAAGACCAA GGAACACTGC AGATTAAGAA TTTACGGATA
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCCA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTAACAGA ATCTGGAGCA ACAATCAGTA AAAATTATGA TATGAATGAC
    CTCCCGGGAC CACCATCCAA ACCTCAGGTC ACTGATGTTT CTAAGAACAG TGTCACCTTA
    TCCTGGCAGC CAGGTACACC TGGCGTTCTT CCTGCAAGCG CGTATATCAT TGAGGCTTTC
    AGCCAATCGG TGAGCAATAG CTGGCAGACA GTGGCAAACC ATGTTAAGAC AACTCTGTAT
    ACAGTAAGGG GGCTGAGGCC CAACACAATC TACTTGTTTA TGGTCAGAGC GATCAACCCA
    CAAGGTCTCA GTGATCCAAG TCCTATGTCG GATCCTGTAC GCACACAAGA TATCAGCCCC
    CCAGCACAAG GAGTGGACCA CAGACAGGTG CAGAAGGAAT TAGGTGATGT CGTTGTTCGT
    CTCCATAATC CAGTTGTCCT GACACCTACA ACTGTTCAAG TCACATGGAC GGTGGACCGA
    CAACCCCAGT TTATTCAGGG CTACAGAGTG ATGTACCGTC AGACTTCGGG ACTACAAGCC
    TCAACTGTGT GGCAGAATCT AGACGCCAAA GTCCCGACTG AGAGGAGTGC TGTCCTTGTG
    AATTTGAAAA AGGGGGTGAC TTATGAAATT AAAGTCCGGC CGTATTTTAA CGAGTTCCAA
    GGAATGGACA GTGAATCGAA AACAGTCCGA ACCACTGAGG AAGCCCCAAG TGCCCCTCCC
    CAGTCTGTCA CTGTGCTGAC AGTTGGAAGT CACAACAGCA CAAGCATCAG TGTTTCCTGG
    GATCCTCCAC CAGCCGACCA CCAGAATGGA ATTATTCAGG AATATAAGAT CTGGTGTCTG
    GGAAACGAAA CGCGATTCCA TATCAATAAA ACGGTGGATG CAGCCATTCG CTCTGTAGTA
    ATAGGTGGCT TGTTCCCTGG AATTCAGTAC CGGGTAGAAG TGGCAGCTAG CACAAGTGCA
    GGGGTTGGAG TAAAAAGTGA ACCACAGCCG ATAATAATTG GGGGACGTAA CGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATCACTGAG CAAATCACGG ATGTCGTGAA GCAACCGGCA
    TTTATAGCTG GCATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATCTGGTTG
    TACTGGAGAA GAAAGAAGAG AAAGGGACTC AGCAATTATG CTGTAACATT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGGAGCCGT CCAGGTCTTC TAAATGCTGG CGATCCCAAT
    TACCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGTTTGC CAGTGAACAA TAGCAATAGT
    GGCCCAAATG AAATTGGAAA TTTTGGGCGT GGAGATGTGC TGCCTCCGGT GCCAGGCCAA
    GGGGATAAAA CAGCGACCAT GCTCTCGGAT GGAGCCATTT ATAGCAGCAT TGACTTCACT
    ACCAAAACCA CTTACAACAG TTCCAGCCAA ATAACACAGG CCACCCCATA TGCCACTACA
    CAAATCCTGC ATTCAAACAG CATCCACGAA CTGGCAGTTG ATCTTCCTGA TCCACAGTGG
    AAAAGCTCAG TTCAACAGAA GACAGACCTC ATGGGATTTG GTTATTCGCT ACCTGATCAG
    AACAAGGGGA ACAACGCCTT ACTTTACATC CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAC TAAAAATTCT
    TCGAAAGCGC AGAAAAACAA CGGATCCACT TGGGCTAATG TCCCTCTACC TCCTCCTCCC
    GTCCAGCCCC TTCCTGGTAC AGAGCTGGGC CACTATGCTG CGGAACAAGA AAATGGCTAT
    GACAGTGACA GCTGGTGCCC ACCATTACCG GTGCAAACAT ACTTGCATCA GGGTATGGAA
    GATGAGCTGG AAGAAGACGA AGATCGGGTC CCAACACCTC CTGTTCGCGG CGTGGCCTCT
    TCACCAGCTA TCTCTTTTGG ACAGCAGTCC ACTGCCACTC TTACTCCATC CCCACGGGAA
    GAGATGCAAC CCATGCTGCA AGCTCACTTG GATGAGTTGA CAAGGGCCTA TCAGTTTGAT
    ATAGCAAAAC AAACATGGCA CATTCAAAGC AATACCCCAC CTCCACAACC CCCAGCTCCG
    CCATTAGGTT ACGTGTCCGG AGCCTTGATT TCTGATTTGG AGACAGATGT TCCAGATGAG
    GATGCTGATG ATGAAGAGGA ACCATTAGAA ATTCCCAGGC CCCTCAGAGC ACTAGACCAG
    ACACCTGGAT CCAGTATGGA CAATCTAGAC AGCTCTGTCA CAGGAAAAGC CTTTAGCTCC
    TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG
    AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG
    CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC
    CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA
    AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC
    ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG
    GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG
    GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG
    CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG
    CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA
    AATAGTCAAG GACAGTTTAC AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_780758.3
    CDS1..4527
    Translation

    Target ORF information:

    RefSeq Version NM_175549.4
    Organism Mus musculus(House mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_175549.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGCTCCTC CACAGTTTGT GGTTAGGCCA
    AGAGATCAGA TCGTTGCTCA AGGCCGAACA GTGACATTCC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCGAATCAA
    CCTCAGCAGC CCAACAGCCG ATGTTCAGTG TCGCCCACGG GGGACCTCAC CATCACCAAC
    ATCCAGCGTT CAGATGCGGG TTACTACATC TGCCAGGCCC TAACCGTGGC AGGAAGCATT
    TTAGCTAAAG CACAGTTGGA AGTTACTGAC GTTTTGACAG ATAGACCTCC ACCCATAATC
    TTGCAAGGAC CAATAAACCA AACACTTGCA GTAGACGGTA CAGCATTGTT GAAGTGTAAA
    GCCACTGGTG AGCCTCTGCC TGTAATTAGC TGGCTAAAGG AGGGCTTTAC TTTTCTGGGG
    AGAGATCCAA GAGCCACGAT CCAAGACCAA GGAACACTGC AGATTAAGAA TTTACGGATA
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCCA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTAACAGA ATCTGGAGCA ACAATCAGTA AAAATTATGA TATGAATGAC
    CTCCCGGGAC CACCATCCAA ACCTCAGGTC ACTGATGTTT CTAAGAACAG TGTCACCTTA
    TCCTGGCAGC CAGGTACACC TGGCGTTCTT CCTGCAAGCG CGTATATCAT TGAGGCTTTC
    AGCCAATCGG TGAGCAATAG CTGGCAGACA GTGGCAAACC ATGTTAAGAC AACTCTGTAT
    ACAGTAAGGG GGCTGAGGCC CAACACAATC TACTTGTTTA TGGTCAGAGC GATCAACCCA
    CAAGGTCTCA GTGATCCAAG TCCTATGTCG GATCCTGTAC GCACACAAGA TATCAGCCCC
    CCAGCACAAG GAGTGGACCA CAGACAGGTG CAGAAGGAAT TAGGTGATGT CGTTGTTCGT
    CTCCATAATC CAGTTGTCCT GACACCTACA ACTGTTCAAG TCACATGGAC GGTGGACCGA
    CAACCCCAGT TTATTCAGGG CTACAGAGTG ATGTACCGTC AGACTTCGGG ACTACAAGCC
    TCAACTGTGT GGCAGAATCT AGACGCCAAA GTCCCGACTG AGAGGAGTGC TGTCCTTGTG
    AATTTGAAAA AGGGGGTGAC TTATGAAATT AAAGTCCGGC CGTATTTTAA CGAGTTCCAA
    GGAATGGACA GTGAATCGAA AACAGTCCGA ACCACTGAGG AAGCCCCAAG TGCCCCTCCC
    CAGTCTGTCA CTGTGCTGAC AGTTGGAAGT CACAACAGCA CAAGCATCAG TGTTTCCTGG
    GATCCTCCAC CAGCCGACCA CCAGAATGGA ATTATTCAGG AATATAAGAT CTGGTGTCTG
    GGAAACGAAA CGCGATTCCA TATCAATAAA ACGGTGGATG CAGCCATTCG CTCTGTAGTA
    ATAGGTGGCT TGTTCCCTGG AATTCAGTAC CGGGTAGAAG TGGCAGCTAG CACAAGTGCA
    GGGGTTGGAG TAAAAAGTGA ACCACAGCCG ATAATAATTG GGGGACGTAA CGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATCACTGAG CAAATCACGG ATGTCGTGAA GCAACCGGCA
    TTTATAGCTG GCATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATCTGGTTG
    TACTGGAGAA GAAAGAAGAG AAAGGGACTC AGCAATTATG CTGTAACATT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGGAGCCGT CCAGGTCTTC TAAATGCTGG CGATCCCAAT
    TACCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGTTTGC CAGTGAACAA TAGCAATAGT
    GGCCCAAATG AAATTGGAAA TTTTGGGCGT GGAGATGTGC TGCCTCCGGT GCCAGGCCAA
    GGGGATAAAA CAGCGACCAT GCTCTCGGAT GGAGCCATTT ATAGCAGCAT TGACTTCACT
    ACCAAAACCA CTTACAACAG TTCCAGCCAA ATAACACAGG CCACCCCATA TGCCACTACA
    CAAATCCTGC ATTCAAACAG CATCCACGAA CTGGCAGTTG ATCTTCCTGA TCCACAGTGG
    AAAAGCTCAG TTCAACAGAA GACAGACCTC ATGGGATTTG GTTATTCGCT ACCTGATCAG
    AACAAGGGGA ACAACGCCTT ACTTTACATC CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAC TAAAAATTCT
    TCGAAAGCGC AGAAAAACAA CGGATCCACT TGGGCTAATG TCCCTCTACC TCCTCCTCCC
    GTCCAGCCCC TTCCTGGTAC AGAGCTGGGC CACTATGCTG CGGAACAAGA AAATGGCTAT
    GACAGTGACA GCTGGTGCCC ACCATTACCG GTGCAAACAT ACTTGCATCA GGGTATGGAA
    GATGAGCTGG AAGAAGACGA AGATCGGGTC CCAACACCTC CTGTTCGCGG CGTGGCCTCT
    TCACCAGCTA TCTCTTTTGG ACAGCAGTCC ACTGCCACTC TTACTCCATC CCCACGGGAA
    GAGATGCAAC CCATGCTGCA AGCTCACTTG GATGAGTTGA CAAGGGCCTA TCAGTTTGAT
    ATAGCAAAAC AAACATGGCA CATTCAAAGC AATACCCCAC CTCCACAACC CCCAGCTCCG
    CCATTAGGTT ACGTGTCCGG AGCCTTGATT TCTGATTTGG AGACAGATGT TCCAGATGAG
    GATGCTGATG ATGAAGAGGA ACCATTAGAA ATTCCCAGGC CCCTCAGAGC ACTAGACCAG
    ACACCTGGAT CCAGTATGGA CAATCTAGAC AGCTCTGTCA CAGGAAAAGC CTTTAGCTCC
    TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG
    AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG
    CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC
    CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA
    AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC
    ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG
    GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG
    GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG
    CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG
    CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA
    AATAGTCAAG GACAGTTTAC AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15146
    Clone ID Related Accession (Same CDS sequence) NM_175549.4 , NM_175549.5
    Accession Version NM_175549.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform 5 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158234.2, AC112155.3, AC134452.4, AC122049.2 and AC162374.4. On Dec 1, 2017 this sequence version replaced NM_175549.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ533876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGCTCCTC CACAGTTTGT GGTTAGGCCA
    AGAGATCAGA TCGTTGCTCA AGGCCGAACA GTGACATTCC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCGAATCAA
    CCTCAGCAGC CCAACAGCCG ATGTTCAGTG TCGCCCACGG GGGACCTCAC CATCACCAAC
    ATCCAGCGTT CAGATGCGGG TTACTACATC TGCCAGGCCC TAACCGTGGC AGGAAGCATT
    TTAGCTAAAG CACAGTTGGA AGTTACTGAC GTTTTGACAG ATAGACCTCC ACCCATAATC
    TTGCAAGGAC CAATAAACCA AACACTTGCA GTAGACGGTA CAGCATTGTT GAAGTGTAAA
    GCCACTGGTG AGCCTCTGCC TGTAATTAGC TGGCTAAAGG AGGGCTTTAC TTTTCTGGGG
    AGAGATCCAA GAGCCACGAT CCAAGACCAA GGAACACTGC AGATTAAGAA TTTACGGATA
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCCA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTAACAGA ATCTGGAGCA ACAATCAGTA AAAATTATGA TATGAATGAC
    CTCCCGGGAC CACCATCCAA ACCTCAGGTC ACTGATGTTT CTAAGAACAG TGTCACCTTA
    TCCTGGCAGC CAGGTACACC TGGCGTTCTT CCTGCAAGCG CGTATATCAT TGAGGCTTTC
    AGCCAATCGG TGAGCAATAG CTGGCAGACA GTGGCAAACC ATGTTAAGAC AACTCTGTAT
    ACAGTAAGGG GGCTGAGGCC CAACACAATC TACTTGTTTA TGGTCAGAGC GATCAACCCA
    CAAGGTCTCA GTGATCCAAG TCCTATGTCG GATCCTGTAC GCACACAAGA TATCAGCCCC
    CCAGCACAAG GAGTGGACCA CAGACAGGTG CAGAAGGAAT TAGGTGATGT CGTTGTTCGT
    CTCCATAATC CAGTTGTCCT GACACCTACA ACTGTTCAAG TCACATGGAC GGTGGACCGA
    CAACCCCAGT TTATTCAGGG CTACAGAGTG ATGTACCGTC AGACTTCGGG ACTACAAGCC
    TCAACTGTGT GGCAGAATCT AGACGCCAAA GTCCCGACTG AGAGGAGTGC TGTCCTTGTG
    AATTTGAAAA AGGGGGTGAC TTATGAAATT AAAGTCCGGC CGTATTTTAA CGAGTTCCAA
    GGAATGGACA GTGAATCGAA AACAGTCCGA ACCACTGAGG AAGCCCCAAG TGCCCCTCCC
    CAGTCTGTCA CTGTGCTGAC AGTTGGAAGT CACAACAGCA CAAGCATCAG TGTTTCCTGG
    GATCCTCCAC CAGCCGACCA CCAGAATGGA ATTATTCAGG AATATAAGAT CTGGTGTCTG
    GGAAACGAAA CGCGATTCCA TATCAATAAA ACGGTGGATG CAGCCATTCG CTCTGTAGTA
    ATAGGTGGCT TGTTCCCTGG AATTCAGTAC CGGGTAGAAG TGGCAGCTAG CACAAGTGCA
    GGGGTTGGAG TAAAAAGTGA ACCACAGCCG ATAATAATTG GGGGACGTAA CGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATCACTGAG CAAATCACGG ATGTCGTGAA GCAACCGGCA
    TTTATAGCTG GCATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATCTGGTTG
    TACTGGAGAA GAAAGAAGAG AAAGGGACTC AGCAATTATG CTGTAACATT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGGAGCCGT CCAGGTCTTC TAAATGCTGG CGATCCCAAT
    TACCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGTTTGC CAGTGAACAA TAGCAATAGT
    GGCCCAAATG AAATTGGAAA TTTTGGGCGT GGAGATGTGC TGCCTCCGGT GCCAGGCCAA
    GGGGATAAAA CAGCGACCAT GCTCTCGGAT GGAGCCATTT ATAGCAGCAT TGACTTCACT
    ACCAAAACCA CTTACAACAG TTCCAGCCAA ATAACACAGG CCACCCCATA TGCCACTACA
    CAAATCCTGC ATTCAAACAG CATCCACGAA CTGGCAGTTG ATCTTCCTGA TCCACAGTGG
    AAAAGCTCAG TTCAACAGAA GACAGACCTC ATGGGATTTG GTTATTCGCT ACCTGATCAG
    AACAAGGGGA ACAACGCCTT ACTTTACATC CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAC TAAAAATTCT
    TCGAAAGCGC AGAAAAACAA CGGATCCACT TGGGCTAATG TCCCTCTACC TCCTCCTCCC
    GTCCAGCCCC TTCCTGGTAC AGAGCTGGGC CACTATGCTG CGGAACAAGA AAATGGCTAT
    GACAGTGACA GCTGGTGCCC ACCATTACCG GTGCAAACAT ACTTGCATCA GGGTATGGAA
    GATGAGCTGG AAGAAGACGA AGATCGGGTC CCAACACCTC CTGTTCGCGG CGTGGCCTCT
    TCACCAGCTA TCTCTTTTGG ACAGCAGTCC ACTGCCACTC TTACTCCATC CCCACGGGAA
    GAGATGCAAC CCATGCTGCA AGCTCACTTG GATGAGTTGA CAAGGGCCTA TCAGTTTGAT
    ATAGCAAAAC AAACATGGCA CATTCAAAGC AATACCCCAC CTCCACAACC CCCAGCTCCG
    CCATTAGGTT ACGTGTCCGG AGCCTTGATT TCTGATTTGG AGACAGATGT TCCAGATGAG
    GATGCTGATG ATGAAGAGGA ACCATTAGAA ATTCCCAGGC CCCTCAGAGC ACTAGACCAG
    ACACCTGGAT CCAGTATGGA CAATCTAGAC AGCTCTGTCA CAGGAAAAGC CTTTAGCTCC
    TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG
    AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG
    CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC
    CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA
    AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC
    ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG
    GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG
    GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG
    CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG
    CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA
    AATAGTCAAG GACAGTTTAC AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_780758.3
    CDS914..5440
    Translation

    Target ORF information:

    RefSeq Version NM_175549.5
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_175549.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGCTCCTC CACAGTTTGT GGTTAGGCCA
    AGAGATCAGA TCGTTGCTCA AGGCCGAACA GTGACATTCC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCGAATCAA
    CCTCAGCAGC CCAACAGCCG ATGTTCAGTG TCGCCCACGG GGGACCTCAC CATCACCAAC
    ATCCAGCGTT CAGATGCGGG TTACTACATC TGCCAGGCCC TAACCGTGGC AGGAAGCATT
    TTAGCTAAAG CACAGTTGGA AGTTACTGAC GTTTTGACAG ATAGACCTCC ACCCATAATC
    TTGCAAGGAC CAATAAACCA AACACTTGCA GTAGACGGTA CAGCATTGTT GAAGTGTAAA
    GCCACTGGTG AGCCTCTGCC TGTAATTAGC TGGCTAAAGG AGGGCTTTAC TTTTCTGGGG
    AGAGATCCAA GAGCCACGAT CCAAGACCAA GGAACACTGC AGATTAAGAA TTTACGGATA
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCCA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTAACAGA ATCTGGAGCA ACAATCAGTA AAAATTATGA TATGAATGAC
    CTCCCGGGAC CACCATCCAA ACCTCAGGTC ACTGATGTTT CTAAGAACAG TGTCACCTTA
    TCCTGGCAGC CAGGTACACC TGGCGTTCTT CCTGCAAGCG CGTATATCAT TGAGGCTTTC
    AGCCAATCGG TGAGCAATAG CTGGCAGACA GTGGCAAACC ATGTTAAGAC AACTCTGTAT
    ACAGTAAGGG GGCTGAGGCC CAACACAATC TACTTGTTTA TGGTCAGAGC GATCAACCCA
    CAAGGTCTCA GTGATCCAAG TCCTATGTCG GATCCTGTAC GCACACAAGA TATCAGCCCC
    CCAGCACAAG GAGTGGACCA CAGACAGGTG CAGAAGGAAT TAGGTGATGT CGTTGTTCGT
    CTCCATAATC CAGTTGTCCT GACACCTACA ACTGTTCAAG TCACATGGAC GGTGGACCGA
    CAACCCCAGT TTATTCAGGG CTACAGAGTG ATGTACCGTC AGACTTCGGG ACTACAAGCC
    TCAACTGTGT GGCAGAATCT AGACGCCAAA GTCCCGACTG AGAGGAGTGC TGTCCTTGTG
    AATTTGAAAA AGGGGGTGAC TTATGAAATT AAAGTCCGGC CGTATTTTAA CGAGTTCCAA
    GGAATGGACA GTGAATCGAA AACAGTCCGA ACCACTGAGG AAGCCCCAAG TGCCCCTCCC
    CAGTCTGTCA CTGTGCTGAC AGTTGGAAGT CACAACAGCA CAAGCATCAG TGTTTCCTGG
    GATCCTCCAC CAGCCGACCA CCAGAATGGA ATTATTCAGG AATATAAGAT CTGGTGTCTG
    GGAAACGAAA CGCGATTCCA TATCAATAAA ACGGTGGATG CAGCCATTCG CTCTGTAGTA
    ATAGGTGGCT TGTTCCCTGG AATTCAGTAC CGGGTAGAAG TGGCAGCTAG CACAAGTGCA
    GGGGTTGGAG TAAAAAGTGA ACCACAGCCG ATAATAATTG GGGGACGTAA CGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATCACTGAG CAAATCACGG ATGTCGTGAA GCAACCGGCA
    TTTATAGCTG GCATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATCTGGTTG
    TACTGGAGAA GAAAGAAGAG AAAGGGACTC AGCAATTATG CTGTAACATT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGGAGCCGT CCAGGTCTTC TAAATGCTGG CGATCCCAAT
    TACCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGTTTGC CAGTGAACAA TAGCAATAGT
    GGCCCAAATG AAATTGGAAA TTTTGGGCGT GGAGATGTGC TGCCTCCGGT GCCAGGCCAA
    GGGGATAAAA CAGCGACCAT GCTCTCGGAT GGAGCCATTT ATAGCAGCAT TGACTTCACT
    ACCAAAACCA CTTACAACAG TTCCAGCCAA ATAACACAGG CCACCCCATA TGCCACTACA
    CAAATCCTGC ATTCAAACAG CATCCACGAA CTGGCAGTTG ATCTTCCTGA TCCACAGTGG
    AAAAGCTCAG TTCAACAGAA GACAGACCTC ATGGGATTTG GTTATTCGCT ACCTGATCAG
    AACAAGGGGA ACAACGCCTT ACTTTACATC CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAC TAAAAATTCT
    TCGAAAGCGC AGAAAAACAA CGGATCCACT TGGGCTAATG TCCCTCTACC TCCTCCTCCC
    GTCCAGCCCC TTCCTGGTAC AGAGCTGGGC CACTATGCTG CGGAACAAGA AAATGGCTAT
    GACAGTGACA GCTGGTGCCC ACCATTACCG GTGCAAACAT ACTTGCATCA GGGTATGGAA
    GATGAGCTGG AAGAAGACGA AGATCGGGTC CCAACACCTC CTGTTCGCGG CGTGGCCTCT
    TCACCAGCTA TCTCTTTTGG ACAGCAGTCC ACTGCCACTC TTACTCCATC CCCACGGGAA
    GAGATGCAAC CCATGCTGCA AGCTCACTTG GATGAGTTGA CAAGGGCCTA TCAGTTTGAT
    ATAGCAAAAC AAACATGGCA CATTCAAAGC AATACCCCAC CTCCACAACC CCCAGCTCCG
    CCATTAGGTT ACGTGTCCGG AGCCTTGATT TCTGATTTGG AGACAGATGT TCCAGATGAG
    GATGCTGATG ATGAAGAGGA ACCATTAGAA ATTCCCAGGC CCCTCAGAGC ACTAGACCAG
    ACACCTGGAT CCAGTATGGA CAATCTAGAC AGCTCTGTCA CAGGAAAAGC CTTTAGCTCC
    TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG
    AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG
    CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC
    CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA
    AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC
    ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG
    GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG
    GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG
    CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG
    CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA
    AATAGTCAAG GACAGTTTAC AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52764
    Clone ID Related Accession (Same CDS sequence) XM_006523044.3 , NM_001358490.1
    Accession Version NM_001358490.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform 4 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC129186.3, AC158234.2, AC112155.3, AC134452.4, AC122049.2 and AC162374.4. On Dec 1, 2017 this sequence version replaced XM_006523044.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ533875.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
    ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT
    CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA
    GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC
    AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG
    CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA
    GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT
    CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA
    GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC
    TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT
    GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA
    ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC
    ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT
    TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG
    CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT
    ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT
    GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA
    GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA
    CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT
    CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC
    CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA
    GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG
    CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC
    ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA
    GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT
    GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA
    GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC
    CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC
    TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC
    CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA
    GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA
    GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA
    GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC
    CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA
    CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA
    ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT
    TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA
    ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG
    TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT
    CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA
    AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA
    GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG
    GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT
    ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT
    ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC
    TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT
    GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC
    CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC
    CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG
    GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC
    AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA
    ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA
    AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC
    AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT
    AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA
    AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG
    AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC
    CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC
    AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT
    GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA
    CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA
    TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT
    GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA
    CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GAAAAGCCTT TAGCTCCTCT
    CAAAGGCAGC GGCCCACCAG CCCATTTTCT ACGGACAGTA ACACCAGTGC TGCCCAGAAT
    CAAAGCCAGA GGCCTCGGCC CACGAAAAAA CATAAGGGAG GACGGATGGA CCCACAGCCA
    GTGTTACCTC ATCGGAGGGA AGGGATGCCA GATGATCTTC CCCCACCACC AGATCCCCCA
    CCAGGTCAGG GTTTAAGGCA GCAGATAGGC CTGAGCCAAC ACTCTGGTAA CGTGGAAAAC
    TCAACAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACAAG CAGCCAACTT AGAAGACACA
    AAGAGCTCAT TGGATTGTCC AGCTAAAACT GTTCTAGAGT GGCAGCGTCA AACCCAGGAC
    TGGATAAACT CTACAGAACG CCAAGAAGAA ACTCGGAAAG CCCCACACAA ACAAGGGGTT
    GGATCAGAGG AGTCCCTGGT GCCCTACAGC AAGCCCAGCT TCCCGTCTCC TGGCGGGCAC
    AGCTCATCAG GAACATCTTC CTCTAAAGGG TCCACTGGCC CTCGGAAGGC TGACGTGCTG
    AGAGGCAGCC ACCAGAGGAA TGCCAATGAC CTTCTCGACA TTGGATATGT GGGCTCAAAT
    AGTCAAGGAC AGTTTACAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001345419.1
    CDS236..4819
    Translation

    Target ORF information:

    RefSeq Version NM_001358490.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001358490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
    ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT
    CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA
    GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC
    AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG
    CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA
    GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT
    CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA
    GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC
    TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT
    GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA
    ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC
    ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT
    TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG
    CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT
    ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT
    GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA
    GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA
    CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT
    CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC
    CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA
    GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG
    CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC
    ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA
    GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT
    GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA
    GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC
    CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC
    TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC
    CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA
    GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA
    GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA
    GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC
    CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA
    CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA
    ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT
    TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA
    ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG
    TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT
    CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA
    AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA
    GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG
    GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT
    ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT
    ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC
    TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT
    GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC
    CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC
    CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG
    GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC
    AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA
    ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA
    AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC
    AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT
    AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA
    AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG
    AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC
    CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC
    AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT
    GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA
    CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA
    TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT
    GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA
    CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GAAAAGCCTT TAGCTCCTCT
    CAAAGGCAGC GGCCCACCAG CCCATTTTCT ACGGACAGTA ACACCAGTGC TGCCCAGAAT
    CAAAGCCAGA GGCCTCGGCC CACGAAAAAA CATAAGGGAG GACGGATGGA CCCACAGCCA
    GTGTTACCTC ATCGGAGGGA AGGGATGCCA GATGATCTTC CCCCACCACC AGATCCCCCA
    CCAGGTCAGG GTTTAAGGCA GCAGATAGGC CTGAGCCAAC ACTCTGGTAA CGTGGAAAAC
    TCAACAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACAAG CAGCCAACTT AGAAGACACA
    AAGAGCTCAT TGGATTGTCC AGCTAAAACT GTTCTAGAGT GGCAGCGTCA AACCCAGGAC
    TGGATAAACT CTACAGAACG CCAAGAAGAA ACTCGGAAAG CCCCACACAA ACAAGGGGTT
    GGATCAGAGG AGTCCCTGGT GCCCTACAGC AAGCCCAGCT TCCCGTCTCC TGGCGGGCAC
    AGCTCATCAG GAACATCTTC CTCTAAAGGG TCCACTGGCC CTCGGAAGGC TGACGTGCTG
    AGAGGCAGCC ACCAGAGGAA TGCCAATGAC CTTCTCGACA TTGGATATGT GGGCTCAAAT
    AGTCAAGGAC AGTTTACAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52767
    Clone ID Related Accession (Same CDS sequence) NM_001358491.1 , XM_006523047.3
    Accession Version NM_001358491.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115120.4, AC158234.2, AC112155.3, AC134452.4, AC122049.2 and AC162374.4. On Dec 2, 2017 this sequence version replaced XM_006523047.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.22491.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC
    CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC
    TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA
    GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG
    CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC
    CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC
    CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT
    CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT
    GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001345420.1
    CDS353..4834
    Translation

    Target ORF information:

    RefSeq Version NM_001358491.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001358491.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC
    CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC
    TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA
    GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG
    CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC
    CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC
    CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT
    CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT
    GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74356
    Clone ID Related Accession (Same CDS sequence) NM_001358492.1 , XM_011246132.2
    Accession Version NM_001358492.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115120.4, AC158234.2, AC112155.3, AC134452.4, AC122049.2 and AC162374.4. On Dec 1, 2017 this sequence version replaced XM_011246132.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.50109.1, SRR1660811.204850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC
    CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA
    TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG
    AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT
    ACAGGTGAAT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001345421.1
    CDS353..4567
    Translation

    Target ORF information:

    RefSeq Version NM_001358492.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001358492.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC
    CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA
    TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG
    AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT
    ACAGGTGAAT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52755
    Clone ID Related Accession (Same CDS sequence) XM_006523035.4 , XM_006523035.3
    Accession Version XM_006523035.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523035.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT
    CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA
    AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG
    AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG
    GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT
    AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT
    GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG
    AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA
    CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT
    CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT
    CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG
    CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC
    ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT
    CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGGTGA ATTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523098.1
    CDS797..5593
    Translation

    Target ORF information:

    RefSeq Version XM_006523035.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523035.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT
    CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA
    AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG
    AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG
    GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT
    AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT
    GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG
    AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA
    CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT
    CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT
    CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG
    CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC
    ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT
    CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGGTGA ATTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52756
    Clone ID Related Accession (Same CDS sequence) XM_006523036.3 , XM_006523036.4
    Accession Version XM_006523036.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT
    CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA
    AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG
    AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG
    GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT
    AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT
    GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG
    AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA
    CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT
    CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT
    CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG
    CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC
    ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT
    CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523099.1
    CDS337..5127
    Translation

    Target ORF information:

    RefSeq Version XM_006523036.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523036.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT
    GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT
    GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA
    TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT
    CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT
    CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG
    ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA
    CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG
    GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG
    CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC
    ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT
    CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA
    AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG
    AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG
    GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT
    AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT
    GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG
    AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA
    CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT
    CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT
    CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG
    CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC
    ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT
    CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52757
    Clone ID Related Accession (Same CDS sequence) XM_006523037.4 , XM_006523037.3
    Accession Version XM_006523037.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523037.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC
    CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC
    ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA
    GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA
    CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG
    AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT
    TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT
    TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT
    TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT
    ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT
    GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA
    ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG
    ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT
    ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC
    GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG
    GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA
    CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT
    GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC
    GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT
    GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT
    GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC
    TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC
    TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG
    CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC
    ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC
    GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT
    AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG
    CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT
    GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT
    GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT
    CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA
    CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT
    CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA
    AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG
    GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC
    GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC
    CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT
    CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA
    CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT
    GTAGGTAGCT CCTCAGATGG CTCCATCTTT GCCAGCGGCA GCTTTGCAAA AGCATTGGTG
    GCAGCAGCAG ACAAAGCTGG CTTTAGGCTG GATGGAACCA GTCTTACGAG AACAGGAAAA
    GCCTTTAGCT CCTCTCAAAG GCAGCGGCCC ACCAGCCCAT TTTCTACGGA CAGTAACACC
    AGTGCTGCCC AGAATCAAAG CCAGAGGCCT CGGCCCACGA AAAAACATAA GGGAGGACGG
    ATGGACCCAC AGCCAGTGTT ACCTCATCGG AGGGAAGGGA TGCCAGATGA TCTTCCCCCA
    CCACCAGATC CCCCACCAGG TCAGGGTTTA AGGCAGCAGA TAGGCCTGAG CCAACACTCT
    GGTAACGTGG AAAACTCAAC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACAAGCAGCC
    AACTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA AAACTGTTCT AGAGTGGCAG
    CGTCAAACCC AGGACTGGAT AAACTCTACA GAACGCCAAG AAGAAACTCG GAAAGCCCCA
    CACAAACAAG GGGTTGGATC AGAGGAGTCC CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG
    TCTCCTGGCG GGCACAGCTC ATCAGGAACA TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG
    AAGGCTGACG TGCTGAGAGG CAGCCACCAG AGGAATGCCA ATGACCTTCT CGACATTGGA
    TATGTGGGCT CAAATAGTCA AGGACAGTTT ACAGGTGAAT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523100.1
    CDS337..5121
    Translation

    Target ORF information:

    RefSeq Version XM_006523037.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523037.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC
    CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC
    ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA
    GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA
    CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG
    AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT
    TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT
    TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT
    TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT
    ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT
    GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA
    ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG
    ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT
    ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC
    GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG
    GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA
    CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT
    GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC
    GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT
    GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT
    GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC
    TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC
    TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC
    ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG
    CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC
    ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC
    GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT
    AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG
    CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT
    GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT
    GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT
    CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA
    CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT
    AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT
    CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA
    AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG
    GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC
    GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC
    CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT
    CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA
    CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT
    GTAGGTAGCT CCTCAGATGG CTCCATCTTT GCCAGCGGCA GCTTTGCAAA AGCATTGGTG
    GCAGCAGCAG ACAAAGCTGG CTTTAGGCTG GATGGAACCA GTCTTACGAG AACAGGAAAA
    GCCTTTAGCT CCTCTCAAAG GCAGCGGCCC ACCAGCCCAT TTTCTACGGA CAGTAACACC
    AGTGCTGCCC AGAATCAAAG CCAGAGGCCT CGGCCCACGA AAAAACATAA GGGAGGACGG
    ATGGACCCAC AGCCAGTGTT ACCTCATCGG AGGGAAGGGA TGCCAGATGA TCTTCCCCCA
    CCACCAGATC CCCCACCAGG TCAGGGTTTA AGGCAGCAGA TAGGCCTGAG CCAACACTCT
    GGTAACGTGG AAAACTCAAC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACAAGCAGCC
    AACTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA AAACTGTTCT AGAGTGGCAG
    CGTCAAACCC AGGACTGGAT AAACTCTACA GAACGCCAAG AAGAAACTCG GAAAGCCCCA
    CACAAACAAG GGGTTGGATC AGAGGAGTCC CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG
    TCTCCTGGCG GGCACAGCTC ATCAGGAACA TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG
    AAGGCTGACG TGCTGAGAGG CAGCCACCAG AGGAATGCCA ATGACCTTCT CGACATTGGA
    TATGTGGGCT CAAATAGTCA AGGACAGTTT ACAGGTGAAT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52758
    Clone ID Related Accession (Same CDS sequence) XM_006523038.3 , XM_006523038.4 , XM_030249140.1 , XM_030249139.1
    Accession Version XM_006523038.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
    ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT
    CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA
    GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC
    AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG
    CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA
    GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT
    CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA
    GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC
    TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT
    GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA
    ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC
    ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT
    TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG
    CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT
    ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT
    GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA
    GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA
    CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT
    CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC
    CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA
    GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG
    CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC
    ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA
    GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT
    GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA
    GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC
    CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC
    TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC
    CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA
    GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA
    GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA
    GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC
    CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA
    CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA
    ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT
    TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA
    ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG
    TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT
    CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA
    AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA
    GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG
    GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT
    ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT
    ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC
    TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT
    GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC
    CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC
    CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG
    GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC
    AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA
    ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA
    AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC
    AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT
    AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA
    AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG
    AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC
    CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC
    AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT
    GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA
    CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA
    TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT
    GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA
    CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG
    GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC
    TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC
    AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC
    TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG
    AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG
    CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC
    CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA
    AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC
    ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG
    GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG
    GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG
    CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG
    CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA
    AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523101.1
    CDS245..5017
    Translation

    Target ORF information:

    RefSeq Version XM_006523038.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523038.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
    ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT
    CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA
    GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC
    AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG
    CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA
    GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT
    CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA
    GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC
    TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT
    GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA
    ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC
    ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT
    TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG
    CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT
    ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT
    GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA
    GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA
    CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT
    CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC
    CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA
    GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG
    CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC
    ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA
    GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT
    GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA
    GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC
    CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC
    TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC
    CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA
    GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA
    GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA
    GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC
    CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA
    CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA
    ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT
    TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA
    ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG
    TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT
    CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA
    AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA
    GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG
    GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT
    ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT
    ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC
    TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT
    GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC
    CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC
    CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG
    GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC
    AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA
    ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA
    AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC
    AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT
    AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA
    AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG
    AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC
    CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC
    AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT
    GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA
    CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA
    TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT
    GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA
    CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG
    GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC
    TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC
    AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC
    TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG
    AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG
    CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC
    CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA
    AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC
    ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG
    GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG
    GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG
    CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG
    CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA
    AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52759
    Clone ID Related Accession (Same CDS sequence) XM_006523039.4 , XM_006523039.3
    Accession Version XM_006523039.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4728bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523039.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC
    ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG
    GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT
    CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG
    TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT
    ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG
    AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC
    AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG
    ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA
    GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA
    GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT
    GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG
    ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG
    GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT
    GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT
    AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA
    AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC
    AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG
    ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT
    CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT
    AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT
    AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG
    AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT
    GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC
    AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG
    GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA
    TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT
    GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG
    CTACCTGATC AGAACAAGGG GAACAACGCC TTACTTTACA TCCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    ACTAAAAATT CTTCGAAAGC GCAGAAAAAC AACGGATCCA CTTGGGCTAA TGTCCCTCTA
    CCTCCTCCTC CCGTCCAGCC CCTTCCTGGT ACAGAGCTGG GCCACTATGC TGCGGAACAA
    GAAAATGGCT ATGACAGTGA CAGCTGGTGC CCACCATTAC CGGTGCAAAC ATACTTGCAT
    CAGGGTATGG AAGATGAGCT GGAAGAAGAC GAAGATCGGG TCCCAACACC TCCTGTTCGC
    GGCGTGGCCT CTTCACCAGC TATCTCTTTT GGACAGCAGT CCACTGCCAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACT TGGATGAGTT GACAAGGGCC
    TATCAGTTTG ATATAGCAAA ACAAACATGG CACATTCAAA GCAATACCCC ACCTCCACAA
    CCCCCAGCTC CGCCATTAGG TTACGTGTCC GGAGCCTTGA TTTCTGATTT GGAGACAGAT
    GTTCCAGATG AGGATGCTGA TGATGAAGAG GAACCATTAG AAATTCCCAG GCCCCTCAGA
    GCACTAGACC AGACACCTGG ATCCAGTATG GACAATCTAG ACAGCTCTGT CACAGGTTCA
    ATGGTAAATG GATGGGGCTC TGCATCTGAT GAGGATCGTA ACTTTTCTAG TCATAGATCT
    AGTGTAGGTA GCTCCTCAGA TGGCTCCATC TTTGCCAGCG GCAGCTTTGC AAAAGCATTG
    GTGGCAGCAG CAGACAAAGC TGGCTTTAGG CTGGATGGAA CCAGTCTTAC GAGAACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC
    CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC
    TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA
    GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG
    CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC
    CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC
    CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT
    CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT
    GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523102.1
    CDS2415..7142
    Translation

    Target ORF information:

    RefSeq Version XM_006523039.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523039.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
    GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC
    ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC
    ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC
    CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA
    AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA
    GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC
    CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA
    ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG
    TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA
    GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT
    AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA
    AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC
    ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG
    GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT
    CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG
    TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT
    ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG
    AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT
    GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC
    AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG
    ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA
    GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA
    GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT
    GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG
    ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG
    GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT
    GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT
    AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA
    AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC
    AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG
    ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT
    CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT
    AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT
    AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG
    AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT
    GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC
    AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG
    GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA
    TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT
    GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG
    CTACCTGATC AGAACAAGGG GAACAACGCC TTACTTTACA TCCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    ACTAAAAATT CTTCGAAAGC GCAGAAAAAC AACGGATCCA CTTGGGCTAA TGTCCCTCTA
    CCTCCTCCTC CCGTCCAGCC CCTTCCTGGT ACAGAGCTGG GCCACTATGC TGCGGAACAA
    GAAAATGGCT ATGACAGTGA CAGCTGGTGC CCACCATTAC CGGTGCAAAC ATACTTGCAT
    CAGGGTATGG AAGATGAGCT GGAAGAAGAC GAAGATCGGG TCCCAACACC TCCTGTTCGC
    GGCGTGGCCT CTTCACCAGC TATCTCTTTT GGACAGCAGT CCACTGCCAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACT TGGATGAGTT GACAAGGGCC
    TATCAGTTTG ATATAGCAAA ACAAACATGG CACATTCAAA GCAATACCCC ACCTCCACAA
    CCCCCAGCTC CGCCATTAGG TTACGTGTCC GGAGCCTTGA TTTCTGATTT GGAGACAGAT
    GTTCCAGATG AGGATGCTGA TGATGAAGAG GAACCATTAG AAATTCCCAG GCCCCTCAGA
    GCACTAGACC AGACACCTGG ATCCAGTATG GACAATCTAG ACAGCTCTGT CACAGGTTCA
    ATGGTAAATG GATGGGGCTC TGCATCTGAT GAGGATCGTA ACTTTTCTAG TCATAGATCT
    AGTGTAGGTA GCTCCTCAGA TGGCTCCATC TTTGCCAGCG GCAGCTTTGC AAAAGCATTG
    GTGGCAGCAG CAGACAAAGC TGGCTTTAGG CTGGATGGAA CCAGTCTTAC GAGAACAGGA
    AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC
    ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA
    CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC
    CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC
    TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA
    GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG
    CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC
    CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC
    CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT
    CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT
    GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52760
    Clone ID Related Accession (Same CDS sequence) XM_006523040.4 , XM_006523040.3
    Accession Version XM_006523040.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523040.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT
    TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA
    TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA
    TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA
    GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT
    AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA
    GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGATCTT
    CCCCCACCAC CAGATCCCCC ACCAGGTCAG GGTTTAAGGC AGCAGATAGG CCTGAGCCAA
    CACTCTGGTA ACGTGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAA
    GCAGCCAACT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAAAAC TGTTCTAGAG
    TGGCAGCGTC AAACCCAGGA CTGGATAAAC TCTACAGAAC GCCAAGAAGA AACTCGGAAA
    GCCCCACACA AACAAGGGGT TGGATCAGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC
    TTCCCGTCTC CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC
    CCTCGGAAGG CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC
    ATTGGATATG TGGGCTCAAA TAGTCAAGGA CAGTTTACAG GTGAATTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523103.1
    CDS337..5007
    Translation

    Target ORF information:

    RefSeq Version XM_006523040.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523040.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA
    CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG
    GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT
    GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA
    GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA
    GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG
    TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG
    CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT
    CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT
    CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG
    GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC
    CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA
    GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT
    ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG
    GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG
    CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT
    AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT
    TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC
    GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC
    CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT
    ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA
    CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA
    TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA
    GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT
    CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT
    GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG
    CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG
    GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC
    ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG
    GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT
    GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA
    GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT
    GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG
    GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT
    GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT
    CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG
    CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG
    CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT
    TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG
    GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT
    GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT
    GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG
    AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT
    CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA
    CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG
    CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT
    CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA
    CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA
    GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC
    AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT
    TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA
    TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA
    TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA
    GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT
    AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA
    GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGATCTT
    CCCCCACCAC CAGATCCCCC ACCAGGTCAG GGTTTAAGGC AGCAGATAGG CCTGAGCCAA
    CACTCTGGTA ACGTGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAA
    GCAGCCAACT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAAAAC TGTTCTAGAG
    TGGCAGCGTC AAACCCAGGA CTGGATAAAC TCTACAGAAC GCCAAGAAGA AACTCGGAAA
    GCCCCACACA AACAAGGGGT TGGATCAGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC
    TTCCCGTCTC CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC
    CCTCGGAAGG CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC
    ATTGGATATG TGGGCTCAAA TAGTCAAGGA CAGTTTACAG GTGAATTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52761
    Clone ID Related Accession (Same CDS sequence) XM_006523041.3 , XM_006523041.4
    Accession Version XM_006523041.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523041.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
    TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT
    TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT
    TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA
    CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT
    CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT
    GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA
    AACCCCACAG ATGTGGTAGT CGCAGC