Adam1b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adam1b
Entrez Gene ID	280667
Full Name	a disintegrin and metallopeptidase domain 1b
Synonyms	Adam1,Ftna
General protein information	Preferred Names a disintegrin and metallopeptidase domain 1b Names disintegrin and metalloproteinase domain-containing protein 1b ADAM 1b PH-30 alpha a disintegrin and metalloproteinase domain 1b fertilin alpha fertilin subunit alpha-b
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
NM_172125.3	NP_742123.2	disintegrin and metalloproteinase domain-containing protein 1b precursor
NM_172125.2	NP_742123.2	disintegrin and metalloproteinase domain-containing protein 1b precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ADAM1B	NP_001193400.1
Bos taurus (cattle)	ADAM1A	NP_001193401.1
Mus musculus (house mouse)	Adam1b	NP_742123.2
Rattus norvegicus (Norway rat)	LOC100360238	XP_003751238.1

Gene Ontology
Bibliography

Related articles in PubMed

Positive selection in the adhesion domain of Mus sperm Adam genes through gene duplications and function-driven gene complex formations.
Grayson P, Civetta A
BMC evolutionary biology13217(2013 Sep)

Mouse sperm lacking ADAM1b/ADAM2 fertilin can fuse with the egg plasma membrane and effect fertilization.
Kim E, Yamashita M, Nakanishi T, Park KE, Kimura M, Kashiwabara S, Baba T
The Journal of biological chemistry281(9)5634-9(2006 Mar)

Possible function of the ADAM1a/ADAM2 Fertilin complex in the appearance of ADAM3 on the sperm surface.
Nishimura H, Kim E, Nakanishi T, Baba T
The Journal of biological chemistry279(33)34957-62(2004 Aug)

Differential localization of ADAM1a and ADAM1b in the endoplasmic reticulum of testicular germ cells and on the surface of epididymal sperm.
Kim E, Nishimura H, Baba T
Biochemical and biophysical research communications304(2)313-9(2003 May)

The ADAM1a and ADAM1b genes, instead of the ADAM1 (fertilin alpha) gene, are localized on mouse chromosome 5.
Nishimura H, Kim E, Fujimori T, Kashiwabara S, Kuroiwa A, Matsuda Y, Baba T
Gene291(1-2)67-76(2002 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that mouse ADAM1b/ADAM2 fertilin may play a crucial role not in the sperm/egg fusion but in the appearance of these two ADAMs on the sperm surface.
Title: Mouse sperm lacking ADAM1b/ADAM2 fertilin can fuse with the egg plasma membrane and effect fertilization.

Localization of ADAM1b in the endoplasmic reticulum of testicular germ cells and on the surface of epididymal sperm.
Title: Differential localization of ADAM1a and ADAM1b in the endoplasmic reticulum of testicular germ cells and on the surface of epididymal sperm.

The following Adam1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adam1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu58182	NM_172125.3 Latest version!	Mus musculus a disintegrin and metallopeptidase domain 1b (Adam1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OMu58182	NM_172125.2	Mus musculus a disintegrin and metallopeptidase domain 1b (Adam1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adam1b ( NM_172125.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_742123.2 Mus musculus a disintegrin and metallopeptidase domain 1b (Adam1b), mRNA.

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CloneID	OMu58182
Clone ID Related Accession (Same CDS sequence)	NM_172125.3 , NM_172125.2
Accession Version	NM_172125.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Mus musculus(house mouse)
Product	disintegrin and metalloproteinase domain-containing protein 1b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK016558.1, AC129215.3, BC140979.1 and BB679367.1. On Jun 26, 2019 this sequence version replaced NM_172125.2. ##Evidence-Data-START## Transcript is intronless :: AK016558.1, BC140979.1 [ECO:0000345] ##Evidence-Data-END##

ATGGAGCGAC TAAAGCTGGG GAAAATCCCA GAGCACTGGT GTATCAGGCT GGTGGCCATG 
TTGCTCTTGG CAATAATTTT TCTCCCGAGC ACGTTCTGTG ACATTGGATC TGTATATAAT 
TCTTCCTATG AAACTGTCAT CCCTGAGAGA CTGCCAGGCA AGGGGGGGAA AGACCCTGGA 
GGGAAGGTGT CCTACATGCT ATTGATGCAA GGCCAAAAGC AGCTGCTTCA CCTCGAGGTA 
AAGGGACACT ACCCTGAGAA TAACTTCCCA GTCTACAGTT ACCACAATGG CATCCTGAGG 
CAAGAAATGC CTCTCCTCTC CCAGGACTGC CACTATGAAG GCTACATGGA AGGGGTGCCA 
GGCTCCTTTG TTTCTGTCAA CATCTGTTCA GGCCTCAGGG GGGTCTTGAT TAAAGAGGAA 
ACATCCTATG GCATTGAGCC CATGCTCTCT TCCAAAAACT TTGAACATGT CCTCTACACC 
ATGGAGCATC AGCCTGTGGT CTCCTGCAGT GTCACTCCCA AAGACAGCCC TGGGGACACC 
AGCCATCCAC CAAGGAGCAG GAAGCCCGAT GACCTACTGG TTCTGACTGA CTGGTGGTCA 
CACACCAAGT ATGTGGAGAT GTTTGTGGTG GTCAACCACC AGCGGTTCCA GATGTGGGGC 
AGTAACATCA ACGAGACGGT CCAGGCAGTA ATGGACATCA TTGCTCTGGC CAACAGCTTC 
ACTAGGGGGA TAAACACAGA GGTGGTGCTG GTGGGCCTGG AAATCTGGAC AGAGGGGGAC 
CCGATAGAGG TCCCAGTGGA CCTGCAGACC ACACTCAGGA ATTTCAACTT CTGGAGACAG 
GAGAAACTCG TGGGCCGGGT CAGGCACGAT GTGGCACACT TGATCGTCGG GCATCGCCCA 
GGAGAGAACG AGGGCCAGGC GTTTCTCCGT GGTGCCTGTT CGGGTGAGTT TGCGGCGGCC 
GTGGAGGCCT TCCATCATGA AGATGTCCTC CTGTTCGCGG CTCTCATGGC CCACGAGCTC 
GGGCACAACC TGGGTATCCA GCACGACCAC CCGACCTGCA CCTGTGGTCC CAAGCACTTC 
TGCCTCATGG GTGAGAAGAT CGGTAAGGAC AGTGGCTTCA GCAACTGCAG CTCTGACCAC 
TTCCTCCGTT TCCTCCACGA CCACAGAGGG GTGTGCCTGC TTGATGAGCC TGGGCGCCAG 
AGCCGCATGC GCAGAGCTGC CAATTGTGGG AATGGTGTGG TGGAGGACTT GGAGCAGTGT 
GACTGTGGCA GTGACTGTGA TAAAAGCCAG TGTTGTGATG AAAATTGTAA ACTGAAGGGC 
AACTCAGTGT GCAGCACTGA ACTTTGCTGT TTTAAATGTA ACTTTAAAAA GGAAGGAGAT 
GTTTGCCGTC CTGCTGACGG ACCATGTGAC CTGGAAGAAT ACTGCAATGG GACCTCTGCA 
GCATGTCCCA GTGACAGGAA AGCCCAGGAC GGCTCTAAGT GCCACGAATC CTTCTTGTGC 
TTTAATGGTC AGTGCATGGA CCCTACCTTT CAGTGCTCTC GTATTTTTGG GCATGGTTCA 
AGGTCTGCCT CCGATTATTG CTACACCTCT CTGAACTCGA GAGGAGACCA GTTTGGAAAC 
TGTGGCTCCT CCTCTCAATT TCCAAAAAAG TATACCAAGT GTTCAGATAA AAATGTAATG 
TGTGGGAAAC TTATATGTAC AGAGGTGGCC TTCCTGCCAC AAATCCAACC GAACAACTTG 
CTGCTCCAGG TCCCTGAGAC CGAGGACTGG TGCTGGAGTG TAGCTGTGTT TGACATGAGA 
GATTCCCTCC ATGAGGAATA CGTGAAGGAC AACACTTACT GTGGCAAAGA TAAAGTCTGT 
AAAAATTCCA TTTGTGAGGA CTTCACTCCA TTCTCATTTC CCTGTAGTCC AAGCAAACAA 
TGCAACAAGC ACGGGGTGTG CAATGACCTG GGGAACTGCC ACTGCTCCTT CGGCTTTGCA 
CCTCCCGACT GCAAAGAGGA AGGCACTGGG GGCAGTGTGG ACAGTGGCCC TGCTGTTAAT 
CTGTCAAATG ACAGCAGTCC AGGGCCGAAC TCCACCCAAT CCAGCACAGA GGAGCTGATC 
CTTAACTTAA AGCTGATCGT CCTGGCTGTC ATTCTGGTGC TCATGATACT CTTAATAATC 
ATATGCATCA TAAGTGCCTA CACCAAGTCA GAGACCGCTT CCGAAGCAGG GCCTTCAGAG 
CTAGAGGAGC TTCCAGAAGG GGAGAAGGAA GAGCAGGAGG AGGTCCTACC AGAAGAGGCT 
AAAGGGGAGG AGGAGGAGTT GGAGTATGGG AAGGAAGAAG CAGAAGAGCA AGGAGCAGTA 
GAAGAGGAAG GAGCAGAAGA GGCAAACGAA GAAGCAGCAG CAGAAAAGAA AGACGAAGAT 
GAAGAAGAGG GAGAGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742123.2
CDS	456..2876
Translation	MERLKLGKIPEHWCIRLVAMLLLAIIFLPSTFCDIGSVYNSSYETVIPERLPGKGGKDPGGKVSYMLLMQGQKQLLHLEVKGHYPENNFPVYSYHNGILRQEMPLLSQDCHYEGYMEGVPGSFVSVNICSGLRGVLIKEETSYGIEPMLSSKNFEHVLYTMEHQPVVSCSVTPKDSPGDTSHPPRSRKPDDLLVLTDWWSHTKYVEMFVVVNHQRFQMWGSNINETVQAVMDIIALANSFTRGINTEVVLVGLEIWTEGDPIEVPVDLQTTLRNFNFWRQEKLVGRVRHDVAHLIVGHRPGENEGQAFLRGACSGEFAAAVEAFHHEDVLLFAALMAHELGHNLGIQHDHPTCTCGPKHFCLMGEKIGKDSGFSNCSSDHFLRFLHDHRGVCLLDEPGRQSRMRRAANCGNGVVEDLEQCDCGSDCDKSQCCDENCKLKGNSVCSTELCCFKCNFKKEGDVCRPADGPCDLEEYCNGTSAACPSDRKAQDGSKCHESFLCFNGQCMDPTFQCSRIFGHGSRSASDYCYTSLNSRGDQFGNCGSSSQFPKKYTKCSDKNVMCGKLICTEVAFLPQIQPNNLLLQVPETEDWCWSVAVFDMRDSLHEEYVKDNTYCGKDKVCKNSICEDFTPFSFPCSPSKQCNKHGVCNDLGNCHCSFGFAPPDCKEEGTGGSVDSGPAVNLSNDSSPGPNSTQSSTEELILNLKLIVLAVILVLMILLIIICIISAYTKSETASEAGPSELEELPEGEKEEQEEVLPEEAKGEEEELEYGKEEAEEQGAVEEEGAEEANEEAAAEKKDEDEEEGEE

Target ORF information:

RefSeq Version	NM_172125.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus a disintegrin and metallopeptidase domain 1b (Adam1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172125.3

ORF Insert Sequence:

ATGGAGCGAC TAAAGCTGGG GAAAATCCCA GAGCACTGGT GTATCAGGCT GGTGGCCATG 
TTGCTCTTGG CAATAATTTT TCTCCCGAGC ACGTTCTGTG ACATTGGATC TGTATATAAT 
TCTTCCTATG AAACTGTCAT CCCTGAGAGA CTGCCAGGCA AGGGGGGGAA AGACCCTGGA 
GGGAAGGTGT CCTACATGCT ATTGATGCAA GGCCAAAAGC AGCTGCTTCA CCTCGAGGTA 
AAGGGACACT ACCCTGAGAA TAACTTCCCA GTCTACAGTT ACCACAATGG CATCCTGAGG 
CAAGAAATGC CTCTCCTCTC CCAGGACTGC CACTATGAAG GCTACATGGA AGGGGTGCCA 
GGCTCCTTTG TTTCTGTCAA CATCTGTTCA GGCCTCAGGG GGGTCTTGAT TAAAGAGGAA 
ACATCCTATG GCATTGAGCC CATGCTCTCT TCCAAAAACT TTGAACATGT CCTCTACACC 
ATGGAGCATC AGCCTGTGGT CTCCTGCAGT GTCACTCCCA AAGACAGCCC TGGGGACACC 
AGCCATCCAC CAAGGAGCAG GAAGCCCGAT GACCTACTGG TTCTGACTGA CTGGTGGTCA 
CACACCAAGT ATGTGGAGAT GTTTGTGGTG GTCAACCACC AGCGGTTCCA GATGTGGGGC 
AGTAACATCA ACGAGACGGT CCAGGCAGTA ATGGACATCA TTGCTCTGGC CAACAGCTTC 
ACTAGGGGGA TAAACACAGA GGTGGTGCTG GTGGGCCTGG AAATCTGGAC AGAGGGGGAC 
CCGATAGAGG TCCCAGTGGA CCTGCAGACC ACACTCAGGA ATTTCAACTT CTGGAGACAG 
GAGAAACTCG TGGGCCGGGT CAGGCACGAT GTGGCACACT TGATCGTCGG GCATCGCCCA 
GGAGAGAACG AGGGCCAGGC GTTTCTCCGT GGTGCCTGTT CGGGTGAGTT TGCGGCGGCC 
GTGGAGGCCT TCCATCATGA AGATGTCCTC CTGTTCGCGG CTCTCATGGC CCACGAGCTC 
GGGCACAACC TGGGTATCCA GCACGACCAC CCGACCTGCA CCTGTGGTCC CAAGCACTTC 
TGCCTCATGG GTGAGAAGAT CGGTAAGGAC AGTGGCTTCA GCAACTGCAG CTCTGACCAC 
TTCCTCCGTT TCCTCCACGA CCACAGAGGG GTGTGCCTGC TTGATGAGCC TGGGCGCCAG 
AGCCGCATGC GCAGAGCTGC CAATTGTGGG AATGGTGTGG TGGAGGACTT GGAGCAGTGT 
GACTGTGGCA GTGACTGTGA TAAAAGCCAG TGTTGTGATG AAAATTGTAA ACTGAAGGGC 
AACTCAGTGT GCAGCACTGA ACTTTGCTGT TTTAAATGTA ACTTTAAAAA GGAAGGAGAT 
GTTTGCCGTC CTGCTGACGG ACCATGTGAC CTGGAAGAAT ACTGCAATGG GACCTCTGCA 
GCATGTCCCA GTGACAGGAA AGCCCAGGAC GGCTCTAAGT GCCACGAATC CTTCTTGTGC 
TTTAATGGTC AGTGCATGGA CCCTACCTTT CAGTGCTCTC GTATTTTTGG GCATGGTTCA 
AGGTCTGCCT CCGATTATTG CTACACCTCT CTGAACTCGA GAGGAGACCA GTTTGGAAAC 
TGTGGCTCCT CCTCTCAATT TCCAAAAAAG TATACCAAGT GTTCAGATAA AAATGTAATG 
TGTGGGAAAC TTATATGTAC AGAGGTGGCC TTCCTGCCAC AAATCCAACC GAACAACTTG 
CTGCTCCAGG TCCCTGAGAC CGAGGACTGG TGCTGGAGTG TAGCTGTGTT TGACATGAGA 
GATTCCCTCC ATGAGGAATA CGTGAAGGAC AACACTTACT GTGGCAAAGA TAAAGTCTGT 
AAAAATTCCA TTTGTGAGGA CTTCACTCCA TTCTCATTTC CCTGTAGTCC AAGCAAACAA 
TGCAACAAGC ACGGGGTGTG CAATGACCTG GGGAACTGCC ACTGCTCCTT CGGCTTTGCA 
CCTCCCGACT GCAAAGAGGA AGGCACTGGG GGCAGTGTGG ACAGTGGCCC TGCTGTTAAT 
CTGTCAAATG ACAGCAGTCC AGGGCCGAAC TCCACCCAAT CCAGCACAGA GGAGCTGATC 
CTTAACTTAA AGCTGATCGT CCTGGCTGTC ATTCTGGTGC TCATGATACT CTTAATAATC 
ATATGCATCA TAAGTGCCTA CACCAAGTCA GAGACCGCTT CCGAAGCAGG GCCTTCAGAG 
CTAGAGGAGC TTCCAGAAGG GGAGAAGGAA GAGCAGGAGG AGGTCCTACC AGAAGAGGCT 
AAAGGGGAGG AGGAGGAGTT GGAGTATGGG AAGGAAGAAG CAGAAGAGCA AGGAGCAGTA 
GAAGAGGAAG GAGCAGAAGA GGCAAACGAA GAAGCAGCAG CAGAAAAGAA AGACGAAGAT 
GAAGAAGAGG GAGAGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adam1b ( NM_172125.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_742123.2 Mus musculus a disintegrin and metallopeptidase domain 1b (Adam1b), mRNA.

Price & Availability↑

CloneID	OMu58182
Clone ID Related Accession (Same CDS sequence)	NM_172125.3 , NM_172125.2
Accession Version	NM_172125.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	disintegrin and metalloproteinase domain-containing protein 1b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC129215.3. On Sep 20, 2006 this sequence version replaced NM_172125.1.

ATGGAGCGAC TAAAGCTGGG GAAAATCCCA GAGCACTGGT GTATCAGGCT GGTGGCCATG 
TTGCTCTTGG CAATAATTTT TCTCCCGAGC ACGTTCTGTG ACATTGGATC TGTATATAAT 
TCTTCCTATG AAACTGTCAT CCCTGAGAGA CTGCCAGGCA AGGGGGGGAA AGACCCTGGA 
GGGAAGGTGT CCTACATGCT ATTGATGCAA GGCCAAAAGC AGCTGCTTCA CCTCGAGGTA 
AAGGGACACT ACCCTGAGAA TAACTTCCCA GTCTACAGTT ACCACAATGG CATCCTGAGG 
CAAGAAATGC CTCTCCTCTC CCAGGACTGC CACTATGAAG GCTACATGGA AGGGGTGCCA 
GGCTCCTTTG TTTCTGTCAA CATCTGTTCA GGCCTCAGGG GGGTCTTGAT TAAAGAGGAA 
ACATCCTATG GCATTGAGCC CATGCTCTCT TCCAAAAACT TTGAACATGT CCTCTACACC 
ATGGAGCATC AGCCTGTGGT CTCCTGCAGT GTCACTCCCA AAGACAGCCC TGGGGACACC 
AGCCATCCAC CAAGGAGCAG GAAGCCCGAT GACCTACTGG TTCTGACTGA CTGGTGGTCA 
CACACCAAGT ATGTGGAGAT GTTTGTGGTG GTCAACCACC AGCGGTTCCA GATGTGGGGC 
AGTAACATCA ACGAGACGGT CCAGGCAGTA ATGGACATCA TTGCTCTGGC CAACAGCTTC 
ACTAGGGGGA TAAACACAGA GGTGGTGCTG GTGGGCCTGG AAATCTGGAC AGAGGGGGAC 
CCGATAGAGG TCCCAGTGGA CCTGCAGACC ACACTCAGGA ATTTCAACTT CTGGAGACAG 
GAGAAACTCG TGGGCCGGGT CAGGCACGAT GTGGCACACT TGATCGTCGG GCATCGCCCA 
GGAGAGAACG AGGGCCAGGC GTTTCTCCGT GGTGCCTGTT CGGGTGAGTT TGCGGCGGCC 
GTGGAGGCCT TCCATCATGA AGATGTCCTC CTGTTCGCGG CTCTCATGGC CCACGAGCTC 
GGGCACAACC TGGGTATCCA GCACGACCAC CCGACCTGCA CCTGTGGTCC CAAGCACTTC 
TGCCTCATGG GTGAGAAGAT CGGTAAGGAC AGTGGCTTCA GCAACTGCAG CTCTGACCAC 
TTCCTCCGTT TCCTCCACGA CCACAGAGGG GTGTGCCTGC TTGATGAGCC TGGGCGCCAG 
AGCCGCATGC GCAGAGCTGC CAATTGTGGG AATGGTGTGG TGGAGGACTT GGAGCAGTGT 
GACTGTGGCA GTGACTGTGA TAAAAGCCAG TGTTGTGATG AAAATTGTAA ACTGAAGGGC 
AACTCAGTGT GCAGCACTGA ACTTTGCTGT TTTAAATGTA ACTTTAAAAA GGAAGGAGAT 
GTTTGCCGTC CTGCTGACGG ACCATGTGAC CTGGAAGAAT ACTGCAATGG GACCTCTGCA 
GCATGTCCCA GTGACAGGAA AGCCCAGGAC GGCTCTAAGT GCCACGAATC CTTCTTGTGC 
TTTAATGGTC AGTGCATGGA CCCTACCTTT CAGTGCTCTC GTATTTTTGG GCATGGTTCA 
AGGTCTGCCT CCGATTATTG CTACACCTCT CTGAACTCGA GAGGAGACCA GTTTGGAAAC 
TGTGGCTCCT CCTCTCAATT TCCAAAAAAG TATACCAAGT GTTCAGATAA AAATGTAATG 
TGTGGGAAAC TTATATGTAC AGAGGTGGCC TTCCTGCCAC AAATCCAACC GAACAACTTG 
CTGCTCCAGG TCCCTGAGAC CGAGGACTGG TGCTGGAGTG TAGCTGTGTT TGACATGAGA 
GATTCCCTCC ATGAGGAATA CGTGAAGGAC AACACTTACT GTGGCAAAGA TAAAGTCTGT 
AAAAATTCCA TTTGTGAGGA CTTCACTCCA TTCTCATTTC CCTGTAGTCC AAGCAAACAA 
TGCAACAAGC ACGGGGTGTG CAATGACCTG GGGAACTGCC ACTGCTCCTT CGGCTTTGCA 
CCTCCCGACT GCAAAGAGGA AGGCACTGGG GGCAGTGTGG ACAGTGGCCC TGCTGTTAAT 
CTGTCAAATG ACAGCAGTCC AGGGCCGAAC TCCACCCAAT CCAGCACAGA GGAGCTGATC 
CTTAACTTAA AGCTGATCGT CCTGGCTGTC ATTCTGGTGC TCATGATACT CTTAATAATC 
ATATGCATCA TAAGTGCCTA CACCAAGTCA GAGACCGCTT CCGAAGCAGG GCCTTCAGAG 
CTAGAGGAGC TTCCAGAAGG GGAGAAGGAA GAGCAGGAGG AGGTCCTACC AGAAGAGGCT 
AAAGGGGAGG AGGAGGAGTT GGAGTATGGG AAGGAAGAAG CAGAAGAGCA AGGAGCAGTA 
GAAGAGGAAG GAGCAGAAGA GGCAAACGAA GAAGCAGCAG CAGAAAAGAA AGACGAAGAT 
GAAGAAGAGG GAGAGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742123.2
CDS	1..2421
Translation	MERLKLGKIPEHWCIRLVAMLLLAIIFLPSTFCDIGSVYNSSYETVIPERLPGKGGKDPGGKVSYMLLMQGQKQLLHLEVKGHYPENNFPVYSYHNGILRQEMPLLSQDCHYEGYMEGVPGSFVSVNICSGLRGVLIKEETSYGIEPMLSSKNFEHVLYTMEHQPVVSCSVTPKDSPGDTSHPPRSRKPDDLLVLTDWWSHTKYVEMFVVVNHQRFQMWGSNINETVQAVMDIIALANSFTRGINTEVVLVGLEIWTEGDPIEVPVDLQTTLRNFNFWRQEKLVGRVRHDVAHLIVGHRPGENEGQAFLRGACSGEFAAAVEAFHHEDVLLFAALMAHELGHNLGIQHDHPTCTCGPKHFCLMGEKIGKDSGFSNCSSDHFLRFLHDHRGVCLLDEPGRQSRMRRAANCGNGVVEDLEQCDCGSDCDKSQCCDENCKLKGNSVCSTELCCFKCNFKKEGDVCRPADGPCDLEEYCNGTSAACPSDRKAQDGSKCHESFLCFNGQCMDPTFQCSRIFGHGSRSASDYCYTSLNSRGDQFGNCGSSSQFPKKYTKCSDKNVMCGKLICTEVAFLPQIQPNNLLLQVPETEDWCWSVAVFDMRDSLHEEYVKDNTYCGKDKVCKNSICEDFTPFSFPCSPSKQCNKHGVCNDLGNCHCSFGFAPPDCKEEGTGGSVDSGPAVNLSNDSSPGPNSTQSSTEELILNLKLIVLAVILVLMILLIIICIISAYTKSETASEAGPSELEELPEGEKEEQEEVLPEEAKGEEEELEYGKEEAEEQGAVEEEGAEEANEEAAAEKKDEDEEEGEE

Target ORF information:

RefSeq Version	NM_172125.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus a disintegrin and metallopeptidase domain 1b (Adam1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172125.2

ORF Insert Sequence:

ATGGAGCGAC TAAAGCTGGG GAAAATCCCA GAGCACTGGT GTATCAGGCT GGTGGCCATG 
TTGCTCTTGG CAATAATTTT TCTCCCGAGC ACGTTCTGTG ACATTGGATC TGTATATAAT 
TCTTCCTATG AAACTGTCAT CCCTGAGAGA CTGCCAGGCA AGGGGGGGAA AGACCCTGGA 
GGGAAGGTGT CCTACATGCT ATTGATGCAA GGCCAAAAGC AGCTGCTTCA CCTCGAGGTA 
AAGGGACACT ACCCTGAGAA TAACTTCCCA GTCTACAGTT ACCACAATGG CATCCTGAGG 
CAAGAAATGC CTCTCCTCTC CCAGGACTGC CACTATGAAG GCTACATGGA AGGGGTGCCA 
GGCTCCTTTG TTTCTGTCAA CATCTGTTCA GGCCTCAGGG GGGTCTTGAT TAAAGAGGAA 
ACATCCTATG GCATTGAGCC CATGCTCTCT TCCAAAAACT TTGAACATGT CCTCTACACC 
ATGGAGCATC AGCCTGTGGT CTCCTGCAGT GTCACTCCCA AAGACAGCCC TGGGGACACC 
AGCCATCCAC CAAGGAGCAG GAAGCCCGAT GACCTACTGG TTCTGACTGA CTGGTGGTCA 
CACACCAAGT ATGTGGAGAT GTTTGTGGTG GTCAACCACC AGCGGTTCCA GATGTGGGGC 
AGTAACATCA ACGAGACGGT CCAGGCAGTA ATGGACATCA TTGCTCTGGC CAACAGCTTC 
ACTAGGGGGA TAAACACAGA GGTGGTGCTG GTGGGCCTGG AAATCTGGAC AGAGGGGGAC 
CCGATAGAGG TCCCAGTGGA CCTGCAGACC ACACTCAGGA ATTTCAACTT CTGGAGACAG 
GAGAAACTCG TGGGCCGGGT CAGGCACGAT GTGGCACACT TGATCGTCGG GCATCGCCCA 
GGAGAGAACG AGGGCCAGGC GTTTCTCCGT GGTGCCTGTT CGGGTGAGTT TGCGGCGGCC 
GTGGAGGCCT TCCATCATGA AGATGTCCTC CTGTTCGCGG CTCTCATGGC CCACGAGCTC 
GGGCACAACC TGGGTATCCA GCACGACCAC CCGACCTGCA CCTGTGGTCC CAAGCACTTC 
TGCCTCATGG GTGAGAAGAT CGGTAAGGAC AGTGGCTTCA GCAACTGCAG CTCTGACCAC 
TTCCTCCGTT TCCTCCACGA CCACAGAGGG GTGTGCCTGC TTGATGAGCC TGGGCGCCAG 
AGCCGCATGC GCAGAGCTGC CAATTGTGGG AATGGTGTGG TGGAGGACTT GGAGCAGTGT 
GACTGTGGCA GTGACTGTGA TAAAAGCCAG TGTTGTGATG AAAATTGTAA ACTGAAGGGC 
AACTCAGTGT GCAGCACTGA ACTTTGCTGT TTTAAATGTA ACTTTAAAAA GGAAGGAGAT 
GTTTGCCGTC CTGCTGACGG ACCATGTGAC CTGGAAGAAT ACTGCAATGG GACCTCTGCA 
GCATGTCCCA GTGACAGGAA AGCCCAGGAC GGCTCTAAGT GCCACGAATC CTTCTTGTGC 
TTTAATGGTC AGTGCATGGA CCCTACCTTT CAGTGCTCTC GTATTTTTGG GCATGGTTCA 
AGGTCTGCCT CCGATTATTG CTACACCTCT CTGAACTCGA GAGGAGACCA GTTTGGAAAC 
TGTGGCTCCT CCTCTCAATT TCCAAAAAAG TATACCAAGT GTTCAGATAA AAATGTAATG 
TGTGGGAAAC TTATATGTAC AGAGGTGGCC TTCCTGCCAC AAATCCAACC GAACAACTTG 
CTGCTCCAGG TCCCTGAGAC CGAGGACTGG TGCTGGAGTG TAGCTGTGTT TGACATGAGA 
GATTCCCTCC ATGAGGAATA CGTGAAGGAC AACACTTACT GTGGCAAAGA TAAAGTCTGT 
AAAAATTCCA TTTGTGAGGA CTTCACTCCA TTCTCATTTC CCTGTAGTCC AAGCAAACAA 
TGCAACAAGC ACGGGGTGTG CAATGACCTG GGGAACTGCC ACTGCTCCTT CGGCTTTGCA 
CCTCCCGACT GCAAAGAGGA AGGCACTGGG GGCAGTGTGG ACAGTGGCCC TGCTGTTAAT 
CTGTCAAATG ACAGCAGTCC AGGGCCGAAC TCCACCCAAT CCAGCACAGA GGAGCTGATC 
CTTAACTTAA AGCTGATCGT CCTGGCTGTC ATTCTGGTGC TCATGATACT CTTAATAATC 
ATATGCATCA TAAGTGCCTA CACCAAGTCA GAGACCGCTT CCGAAGCAGG GCCTTCAGAG 
CTAGAGGAGC TTCCAGAAGG GGAGAAGGAA GAGCAGGAGG AGGTCCTACC AGAAGAGGCT 
AAAGGGGAGG AGGAGGAGTT GGAGTATGGG AAGGAAGAAG CAGAAGAGCA AGGAGCAGTA 
GAAGAGGAAG GAGCAGAAGA GGCAAACGAA GAAGCAGCAG CAGAAAAGAA AGACGAAGAT 
GAAGAAGAGG GAGAGGAATA A