Gene Symbol | Lcmt1 |
Entrez Gene ID | 30949 |
Full Name | leucine carboxyl methyltransferase 1 |
Synonyms | AL033290,LCMT-1,Lcmt |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
ORF » Species Summary » Mus musculus » Lcmt1 cDNA ORF clone
Gene Symbol | Lcmt1 |
Entrez Gene ID | 30949 |
Full Name | leucine carboxyl methyltransferase 1 |
Synonyms | AL033290,LCMT-1,Lcmt |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
mRNA | Protein | Name |
---|---|---|
XM_030242666.1 | XP_030098526.1 | leucine carboxyl methyltransferase 1 isoform X1 |
XM_006507917.3 | XP_006507980.1 | leucine carboxyl methyltransferase 1 isoform X1 |
NM_001355050.1 | NP_001341979.1 | leucine carboxyl methyltransferase 1 isoform 2 |
NM_025304.3 | NP_079580.2 | leucine carboxyl methyltransferase 1 isoform 1 |
LCMT1 | XP_001099127.1 | |
Drosophila melanogaster (fruit fly) | CG3793 | NP_609766.1 |
Caenorhabditis elegans (roundworm) | CELE_B0285.4 | NP_497875.1 |
Oryza sativa (rice) | Os06g0140100 | NP_001056749.1 |
Xenopus tropicalis (tropical clawed frog) | lcmt1 | NP_001016033.1 |
Pan troglodytes (chimpanzee) | LCMT1 | XP_001164891.1 |
Canis lupus familiaris (dog) | LCMT1 | XP_005621453.1 |
Bos taurus (cattle) | LCMT1 | NP_001069956.1 |
Mus musculus (house mouse) | Lcmt1 | NP_079580.2 |
Gallus gallus (chicken) | LCMT1 | NP_001006167.2 |
Arabidopsis thaliana (thale cress) | AT1G02100 | NP_973741.1 |
Anopheles gambiae (African malaria mosquito) | AgaP_AGAP008768 | XP_314892.4 |
Homo sapiens (human) | LCMT1 | NP_057393.2 |
Rattus norvegicus (Norway rat) | Lcmt1 | NP_955437.1 |
Danio rerio (zebrafish) | lcmt1 | NP_001004645.1 |
Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.
Hwang J, Lee JA, Pallas DC
The Journal of biological chemistry291(40)21008-21019(2016 Sep)
Circumventing embryonic lethality with Lcmt1 deficiency: generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling.
MacKay KB, Tu Y, Young SG, Clarke SG
PloS one8(6)e65967(2013)
Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1 plays a critical role in differentiation of neuroblastoma cells.
Sontag JM, Nunbhakdi-Craig V, Mitterhuber M, Ogris E, Sontag E
Journal of neurochemistry115(6)1455-65(2010 Dec)
Leucine carboxyl methyltransferase-1 is necessary for normal progression through mitosis in mammalian cells.
Lee JA, Pallas DC
The Journal of biological chemistry282(42)30974-84(2007 Oct)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
LCMT-1 homozygous knock-out MEFs exhibited hyperphosphorylation of HDAC3, a reported target of the methylation-dependent PP4R1-PP4c complex. Collectively, our data suggest that LCMT-1 coordinately regulates the carboxyl methylation of PP2A-related phosphatases and, consequently, their holoenzyme assembly and function.
Title: Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.
In LCMT1 deficiency enzyme activity and methylation of PP2A are reduced in a coordinate fashion, suggesting that LCMT1 is the only PP2A methyltransferase.
Title: Circumventing embryonic lethality with Lcmt1 deficiency: generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling.
Enhanced expression of LCMT1 in cultured neuroblastoma cells, which increases endogenous methylated catalytic C subunit and Balpha levels, induces changes in F-actin organization.
Title: Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1 plays a critical role in differentiation of neuroblastoma cells.
LCMT-1 is important for normal progression through mitosis and cell survival and is essential for embryonic development
Title: Leucine carboxyl methyltransferase-1 is necessary for normal progression through mitosis in mammalian cells.
The following Lcmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lcmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OMu00244 | |
Clone ID Related Accession (Same CDS sequence) | NM_025304.3 | |
Accession Version | NM_025304.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-01-26 | |
Organism | Mus musculus(house mouse) | |
Product | leucine carboxyl methyltransferase 1 isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU571243.1, AK003424.1 and BB686087.1. On or before Mar 23, 2004 this sequence version replaced XM_355938.1, NM_025304.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.249183.1, AK003424.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGGCCTCCA GCTCGAGGGA GTCCTCGTTC TCTTCCTGCT CGTCGAGCTG TGACTTGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_079580.2 |
CDS | 168..1166 |
Translation |
Target ORF information:
Target ORF information:
|
NM_025304.3 |
1 | ATGGCCTCCA GCTCGAGGGA GTCCTCGTTC TCTTCCTGCT CGTCGAGCTG TGACTTGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu39703 | |
Clone ID Related Accession (Same CDS sequence) | XM_030242666.1 , XM_006507917.3 , NM_001355050.1 | |
Accession Version | NM_001355050.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-01-26 | |
Organism | Mus musculus(house mouse) | |
Product | leucine carboxyl methyltransferase 1 isoform 2 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121956.3. On Sep 6, 2017 this sequence version replaced XM_006507917.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BG870400.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## |
1 | ATGCTGGATT CAAAAAGATA TGCCATCATT GGAGCAGATC TCCGAGACCT TTCTGAGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001341979.1 |
CDS | 628..1158 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001355050.1 |
1 | ATGCTGGATT CAAAAAGATA TGCCATCATT GGAGCAGATC TCCGAGACCT TTCTGAGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu39703 | |
Clone ID Related Accession (Same CDS sequence) | XM_030242666.1 , XM_006507917.3 , NM_001355050.1 | |
Accession Version | XM_006507917.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(House mouse) | |
Product | leucine carboxyl methyltransferase 1 isoform X1 | |
Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507917.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGCTGGATT CAAAAAGATA TGCCATCATT GGAGCAGATC TCCGAGACCT TTCTGAGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006507980.1 |
CDS | 628..1158 |
Translation |
Target ORF information:
Target ORF information:
|
XM_006507917.3 |
1 | ATGCTGGATT CAAAAAGATA TGCCATCATT GGAGCAGATC TCCGAGACCT TTCTGAGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu39703 | |
Clone ID Related Accession (Same CDS sequence) | XM_030242666.1 , XM_006507917.3 , NM_001355050.1 | |
Accession Version | XM_030242666.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | leucine carboxyl methyltransferase 1 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGCTGGATT CAAAAAGATA TGCCATCATT GGAGCAGATC TCCGAGACCT TTCTGAGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_030098526.1 |
CDS | 1434..1964 |
Translation |
Target ORF information:
Target ORF information:
|
XM_030242666.1 |
1 | ATGCTGGATT CAAAAAGATA TGCCATCATT GGAGCAGATC TCCGAGACCT TTCTGAGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes. |
Circumventing embryonic lethality with Lcmt1 deficiency: generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling. |
Regulation of protein phosphatase 2A methylation by LCMT1 and PME-1 plays a critical role in differentiation of neuroblastoma cells. |
Leucine carboxyl methyltransferase-1 is necessary for normal progression through mitosis in mammalian cells. |