Dgki cDNA ORF clone, Mus musculus(house mouse)

The following Dgki gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dgki cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu81755 XM_017321631.2
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu81756 XM_017321632.2
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu81757 XM_017321633.2
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu89227 XM_030255454.1
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu37360 XM_006506257.4
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu37361 XM_006506258.4
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu37362 XM_006506259.4
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu74609 XM_011241385.3
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu81748 XM_017321624.2
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu81749 XM_017321625.2
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu81750 XM_017321626.2
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu81751 XM_017321627.2
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu81752 XM_017321628.2
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu81753 XM_017321629.2
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu81754 XM_017321630.2
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu89226 XM_030255453.1
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu01881 NM_001081206.2
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu37363 NM_001347104.2
Latest version!
Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
View Old Accession Versions >>
OMu37360 XM_006506257.3 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu37361 XM_006506258.3 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu81755 XM_017321631.1 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu37363 NM_001347104.1 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu81749 XM_017321625.1 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu81751 XM_017321627.1 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu81753 XM_017321629.1 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu81748 XM_017321624.1 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu37362 XM_006506259.3 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu81754 XM_017321630.1 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu74609 XM_011241385.2 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu81750 XM_017321626.1 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu81757 XM_017321633.1 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OMu01881 NM_001081206.1 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu81752 XM_017321628.1 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OMu81756 XM_017321632.1 Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu81755
    Clone ID Related Accession (Same CDS sequence) XM_017321631.1 , XM_017321631.2
    Accession Version XM_017321631.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGATAC CACACTTAGG ACTGAGTTTT CCAAGGAAAG CCATCTCCCG GACAGGCCTC 
    CAGCATCTGG CACCTGCACA TCCTCTTGGT CTTCCTGTGG CTAACGGTCC AGCGAAGGAG
    CCCAGAGCCA CTCTGGACTG GAGCGAGAAT GCTGTGAATG GGGAGCACCT GTGGCTGGAG
    ACCAACGTTT CAGGGGACCT CTGCTACCTG GGGGAGGAGA ACTGCCAAGT CCGATTTGCA
    AAATCCGCTC TCAGGAGGAA GTGTGCCGTC TGCAAAATCG TGGTCCACAC AGCCTGCATT
    GAGCAACTAG AGAAGATTAA TTTCAGATGT AAACCAACAT TTCGGGAAGG AGGCTCCAGA
    TCGCCAAGAG AAAACTTTGT GCGTCACCAC TGGGTCCACA GGAGGCGGCA GGAGGGAAAA
    TGTAAGCAGT GTGGTAAGGG CTTCCAGCAA AAGTTCTCCT TCCACAGTAA AGAGATCGTG
    GCCATCAGCT GTTCTTGGTG CAAGCAGGCG TTTCACAATA AAGTGACCTG CTTCATGCTG
    CATCATATTG AGGAACCCTG CTCCCTGGGG GCTCACGCCG CTGTTATTGT CCCGCCCACC
    TGGATCATTA AGGTGAAGAA ACCTCAGGTG ATCTCCATAC CAGGAGATGA ACTTGAACTT
    GGTTGGGCGG GAGACGGACC TGGTTGGGCG GGAGACTGGA GCCCCTCAGA GAATTTGGGA
    TTCTTCCATT TTTACTGGGA GGGTTGGCAG AACTCCCTGA AGGCTTCCAA CCGGAAGAAG
    AAGAGGACAA GTTTTAAAAG GAAAGCCAGT AAAAGAGGAA CTGAGCAGGA AACCAAAGGC
    CGTCCTTTTG TGATCAAGCC CATCTCCTCC CCTCTCATGA AGCCTTTGCT TGTCTTTGTG
    AACCCAAAGA GTGGGGGAAA CCAGGGAACC AAAGTCCTAC AGATGTTTAT GTGGTACCTG
    AATCCACGGC AAGTCTTTGA TCTTTCTCAG GAAGGGCCAA AAGATGCGCT GGAGATGTAC
    AGGAAGGTAC CAAATCTGAG AATTCTGGCC TGCGGTGGGG ATGGAACGGT GGGCTGGATC
    CTCTCCATCC TGGACGAGCT GCAGCTGAGC CCACAGCCTC CTGTGGGGGT GCTTCCACTG
    GGGACTGGGA ATGACCTGGC TCGGACGCTC AACTGGGGAG GGGGTTACAC CGATGAGCCT
    GTTTCTAAGA TCCTGTGCCA GGTGGAAGAT GGGACCATTG TACAGTTGGA TCGCTGGAAC
    CTCCACGTAG AGAGAAACCC AGACCTGCCG CCCGAAGAAC TTGAAGATGG CGTGTGTAAG
    CTTCCTCTGA ATGTCTTCAA TAATTACTTC AGCCTTGGAT TTGATGCTCA CGTCACATTG
    GAGTTCCATG AATCCCGAGA AGCAAATCCA GAGAAATTCA ATAGTCGTTT TCGAAATAAA
    ATGTTCTATG CAGGGGCAGC CTTTTCTGAT TTCCTACAAA GAAGTTCTAG AGATCTATCC
    AAACACGTCA AAGTTGTCTG TGATGGGACA GACCTCACCC CAAAGATCCA GGACCTGAAG
    TTCCAGTGTA TAGTATTTTT AAATATTCCC AGATATTGTG CTGGCACAAT GCCTTGGGGA
    AACCCTGGAG ATCATCATGA CTTTGAACCC CAACGTCATG ATGACGGCTA CATTGAAGTC
    ATTGGATTCA CTATGGCCTC TTTGGCTGCC CTGCAAGTTG GGGGGCACGG AGAAAGGCTG
    CACCAGTGTC GGGAAGTGAT GCTTCTGACT TACAAGTCCA TTCCCATGCA AGTGGACGGG
    GAGCCATGTC GGTTGGCACC TGCTATGATT CGGATCTCCT TGAGGAATCA GGCCAACATG
    GTACAGAAGA GCAAGCGGAG AACCTCGATG CCTTTGCTCA ATGACATCCA TCAGGTGCAA
    GCTGCGGACC TGCGGCGAGT GTCTGCTCCC CCCGGCTCCT TCACCATTCC CCAGTCTGTC
    CCAGATCGCC TGAGGATCCG TGTGAACAAA ATCAGTTTAC AAGACTATGA AGGACTCCAC
    TATGACAAGG ACAAACTCCG GGAAGCTTCC ATCCCTCTGG GTATTCTAGT TGTTCGTGGA
    GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGTC TACAGGAGGA CTTGCAGTCA
    GTTTCTTCTG GGTCACAGAG AGTTCATTAC CAGGACCAAG AAACCTCCTT CCCCAGGGCA
    CTCTCAGCTC AGAGGCTGTC CCCACGGTGG TGCTTCCTAG ATGCAACGTC TGCTGACCGC
    TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCTCACGAT
    GAAATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC AGGCAGGGAC CCCTCCAGGA
    ATGCCTGACC TGGTGGTTGA ACAGGCTTCT GGGCTGTCTG ACTGGTGGAA CCCTGCCCTG
    AGGAAACGTA TGCTGAGTGA CAGTGGGATG ATCACACCCC ACTATGAGGA CTCAGATCTG
    AAAGACTTCA GCCACTCCAG AGTGCTACAG TCCCCAGTCT CTTCAGAAGA TCATGCAATT
    TTGCAGGCAG TCCTCACCGG TGACCTTATG AAGCTCATGG AGAGCTATAA GAATGGAGGG
    AGCTTACTAA TTCAGGGACC AGGGCACTGT TCTCTCCTTC ACTACGCAGC TAAGACCGGC
    AATGGGGACA TTGTGAAGTA CATCCTTGAC CACGGACCTG CAGAATTACT GGATATGGCA
    GACAGTGAAA CGGGCGAGAC TGCACTGCAC AAGGCTGCCT GCCAAAGGAA CCGGGCTGTA
    TGCCAGCTTC TGGTGGATGC AGGAGCATCT CTGAGACAGA CAGACTCCAA GGGCAAGACA
    CCCCAGGAAC GAGCACAGCA GGCCGGGGAC CCAGATTTGG CTGCTTACCT AGAAAGCCGA
    CAGAACTATA AGATCATTGG CCATGAAGAC CTGGAAACTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177120.1
    CDS9168..12155
    Translation

    Target ORF information:

    RefSeq Version XM_017321631.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321631.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGATAC CACACTTAGG ACTGAGTTTT CCAAGGAAAG CCATCTCCCG GACAGGCCTC 
    CAGCATCTGG CACCTGCACA TCCTCTTGGT CTTCCTGTGG CTAACGGTCC AGCGAAGGAG
    CCCAGAGCCA CTCTGGACTG GAGCGAGAAT GCTGTGAATG GGGAGCACCT GTGGCTGGAG
    ACCAACGTTT CAGGGGACCT CTGCTACCTG GGGGAGGAGA ACTGCCAAGT CCGATTTGCA
    AAATCCGCTC TCAGGAGGAA GTGTGCCGTC TGCAAAATCG TGGTCCACAC AGCCTGCATT
    GAGCAACTAG AGAAGATTAA TTTCAGATGT AAACCAACAT TTCGGGAAGG AGGCTCCAGA
    TCGCCAAGAG AAAACTTTGT GCGTCACCAC TGGGTCCACA GGAGGCGGCA GGAGGGAAAA
    TGTAAGCAGT GTGGTAAGGG CTTCCAGCAA AAGTTCTCCT TCCACAGTAA AGAGATCGTG
    GCCATCAGCT GTTCTTGGTG CAAGCAGGCG TTTCACAATA AAGTGACCTG CTTCATGCTG
    CATCATATTG AGGAACCCTG CTCCCTGGGG GCTCACGCCG CTGTTATTGT CCCGCCCACC
    TGGATCATTA AGGTGAAGAA ACCTCAGGTG ATCTCCATAC CAGGAGATGA ACTTGAACTT
    GGTTGGGCGG GAGACGGACC TGGTTGGGCG GGAGACTGGA GCCCCTCAGA GAATTTGGGA
    TTCTTCCATT TTTACTGGGA GGGTTGGCAG AACTCCCTGA AGGCTTCCAA CCGGAAGAAG
    AAGAGGACAA GTTTTAAAAG GAAAGCCAGT AAAAGAGGAA CTGAGCAGGA AACCAAAGGC
    CGTCCTTTTG TGATCAAGCC CATCTCCTCC CCTCTCATGA AGCCTTTGCT TGTCTTTGTG
    AACCCAAAGA GTGGGGGAAA CCAGGGAACC AAAGTCCTAC AGATGTTTAT GTGGTACCTG
    AATCCACGGC AAGTCTTTGA TCTTTCTCAG GAAGGGCCAA AAGATGCGCT GGAGATGTAC
    AGGAAGGTAC CAAATCTGAG AATTCTGGCC TGCGGTGGGG ATGGAACGGT GGGCTGGATC
    CTCTCCATCC TGGACGAGCT GCAGCTGAGC CCACAGCCTC CTGTGGGGGT GCTTCCACTG
    GGGACTGGGA ATGACCTGGC TCGGACGCTC AACTGGGGAG GGGGTTACAC CGATGAGCCT
    GTTTCTAAGA TCCTGTGCCA GGTGGAAGAT GGGACCATTG TACAGTTGGA TCGCTGGAAC
    CTCCACGTAG AGAGAAACCC AGACCTGCCG CCCGAAGAAC TTGAAGATGG CGTGTGTAAG
    CTTCCTCTGA ATGTCTTCAA TAATTACTTC AGCCTTGGAT TTGATGCTCA CGTCACATTG
    GAGTTCCATG AATCCCGAGA AGCAAATCCA GAGAAATTCA ATAGTCGTTT TCGAAATAAA
    ATGTTCTATG CAGGGGCAGC CTTTTCTGAT TTCCTACAAA GAAGTTCTAG AGATCTATCC
    AAACACGTCA AAGTTGTCTG TGATGGGACA GACCTCACCC CAAAGATCCA GGACCTGAAG
    TTCCAGTGTA TAGTATTTTT AAATATTCCC AGATATTGTG CTGGCACAAT GCCTTGGGGA
    AACCCTGGAG ATCATCATGA CTTTGAACCC CAACGTCATG ATGACGGCTA CATTGAAGTC
    ATTGGATTCA CTATGGCCTC TTTGGCTGCC CTGCAAGTTG GGGGGCACGG AGAAAGGCTG
    CACCAGTGTC GGGAAGTGAT GCTTCTGACT TACAAGTCCA TTCCCATGCA AGTGGACGGG
    GAGCCATGTC GGTTGGCACC TGCTATGATT CGGATCTCCT TGAGGAATCA GGCCAACATG
    GTACAGAAGA GCAAGCGGAG AACCTCGATG CCTTTGCTCA ATGACATCCA TCAGGTGCAA
    GCTGCGGACC TGCGGCGAGT GTCTGCTCCC CCCGGCTCCT TCACCATTCC CCAGTCTGTC
    CCAGATCGCC TGAGGATCCG TGTGAACAAA ATCAGTTTAC AAGACTATGA AGGACTCCAC
    TATGACAAGG ACAAACTCCG GGAAGCTTCC ATCCCTCTGG GTATTCTAGT TGTTCGTGGA
    GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGTC TACAGGAGGA CTTGCAGTCA
    GTTTCTTCTG GGTCACAGAG AGTTCATTAC CAGGACCAAG AAACCTCCTT CCCCAGGGCA
    CTCTCAGCTC AGAGGCTGTC CCCACGGTGG TGCTTCCTAG ATGCAACGTC TGCTGACCGC
    TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCTCACGAT
    GAAATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC AGGCAGGGAC CCCTCCAGGA
    ATGCCTGACC TGGTGGTTGA ACAGGCTTCT GGGCTGTCTG ACTGGTGGAA CCCTGCCCTG
    AGGAAACGTA TGCTGAGTGA CAGTGGGATG ATCACACCCC ACTATGAGGA CTCAGATCTG
    AAAGACTTCA GCCACTCCAG AGTGCTACAG TCCCCAGTCT CTTCAGAAGA TCATGCAATT
    TTGCAGGCAG TCCTCACCGG TGACCTTATG AAGCTCATGG AGAGCTATAA GAATGGAGGG
    AGCTTACTAA TTCAGGGACC AGGGCACTGT TCTCTCCTTC ACTACGCAGC TAAGACCGGC
    AATGGGGACA TTGTGAAGTA CATCCTTGAC CACGGACCTG CAGAATTACT GGATATGGCA
    GACAGTGAAA CGGGCGAGAC TGCACTGCAC AAGGCTGCCT GCCAAAGGAA CCGGGCTGTA
    TGCCAGCTTC TGGTGGATGC AGGAGCATCT CTGAGACAGA CAGACTCCAA GGGCAAGACA
    CCCCAGGAAC GAGCACAGCA GGCCGGGGAC CCAGATTTGG CTGCTTACCT AGAAAGCCGA
    CAGAACTATA AGATCATTGG CCATGAAGAC CTGGAAACTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81755
    Clone ID Related Accession (Same CDS sequence) XM_017321631.1 , XM_017321631.2
    Accession Version XM_017321631.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321631.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGATAC CACACTTAGG ACTGAGTTTT CCAAGGAAAG CCATCTCCCG GACAGGCCTC 
    CAGCATCTGG CACCTGCACA TCCTCTTGGT CTTCCTGTGG CTAACGGTCC AGCGAAGGAG
    CCCAGAGCCA CTCTGGACTG GAGCGAGAAT GCTGTGAATG GGGAGCACCT GTGGCTGGAG
    ACCAACGTTT CAGGGGACCT CTGCTACCTG GGGGAGGAGA ACTGCCAAGT CCGATTTGCA
    AAATCCGCTC TCAGGAGGAA GTGTGCCGTC TGCAAAATCG TGGTCCACAC AGCCTGCATT
    GAGCAACTAG AGAAGATTAA TTTCAGATGT AAACCAACAT TTCGGGAAGG AGGCTCCAGA
    TCGCCAAGAG AAAACTTTGT GCGTCACCAC TGGGTCCACA GGAGGCGGCA GGAGGGAAAA
    TGTAAGCAGT GTGGTAAGGG CTTCCAGCAA AAGTTCTCCT TCCACAGTAA AGAGATCGTG
    GCCATCAGCT GTTCTTGGTG CAAGCAGGCG TTTCACAATA AAGTGACCTG CTTCATGCTG
    CATCATATTG AGGAACCCTG CTCCCTGGGG GCTCACGCCG CTGTTATTGT CCCGCCCACC
    TGGATCATTA AGGTGAAGAA ACCTCAGGTG ATCTCCATAC CAGGAGATGA ACTTGAACTT
    GGTTGGGCGG GAGACGGACC TGGTTGGGCG GGAGACTGGA GCCCCTCAGA GAATTTGGGA
    TTCTTCCATT TTTACTGGGA GGGTTGGCAG AACTCCCTGA AGGCTTCCAA CCGGAAGAAG
    AAGAGGACAA GTTTTAAAAG GAAAGCCAGT AAAAGAGGAA CTGAGCAGGA AACCAAAGGC
    CGTCCTTTTG TGATCAAGCC CATCTCCTCC CCTCTCATGA AGCCTTTGCT TGTCTTTGTG
    AACCCAAAGA GTGGGGGAAA CCAGGGAACC AAAGTCCTAC AGATGTTTAT GTGGTACCTG
    AATCCACGGC AAGTCTTTGA TCTTTCTCAG GAAGGGCCAA AAGATGCGCT GGAGATGTAC
    AGGAAGGTAC CAAATCTGAG AATTCTGGCC TGCGGTGGGG ATGGAACGGT GGGCTGGATC
    CTCTCCATCC TGGACGAGCT GCAGCTGAGC CCACAGCCTC CTGTGGGGGT GCTTCCACTG
    GGGACTGGGA ATGACCTGGC TCGGACGCTC AACTGGGGAG GGGGTTACAC CGATGAGCCT
    GTTTCTAAGA TCCTGTGCCA GGTGGAAGAT GGGACCATTG TACAGTTGGA TCGCTGGAAC
    CTCCACGTAG AGAGAAACCC AGACCTGCCG CCCGAAGAAC TTGAAGATGG CGTGTGTAAG
    CTTCCTCTGA ATGTCTTCAA TAATTACTTC AGCCTTGGAT TTGATGCTCA CGTCACATTG
    GAGTTCCATG AATCCCGAGA AGCAAATCCA GAGAAATTCA ATAGTCGTTT TCGAAATAAA
    ATGTTCTATG CAGGGGCAGC CTTTTCTGAT TTCCTACAAA GAAGTTCTAG AGATCTATCC
    AAACACGTCA AAGTTGTCTG TGATGGGACA GACCTCACCC CAAAGATCCA GGACCTGAAG
    TTCCAGTGTA TAGTATTTTT AAATATTCCC AGATATTGTG CTGGCACAAT GCCTTGGGGA
    AACCCTGGAG ATCATCATGA CTTTGAACCC CAACGTCATG ATGACGGCTA CATTGAAGTC
    ATTGGATTCA CTATGGCCTC TTTGGCTGCC CTGCAAGTTG GGGGGCACGG AGAAAGGCTG
    CACCAGTGTC GGGAAGTGAT GCTTCTGACT TACAAGTCCA TTCCCATGCA AGTGGACGGG
    GAGCCATGTC GGTTGGCACC TGCTATGATT CGGATCTCCT TGAGGAATCA GGCCAACATG
    GTACAGAAGA GCAAGCGGAG AACCTCGATG CCTTTGCTCA ATGACATCCA TCAGGTGCAA
    GCTGCGGACC TGCGGCGAGT GTCTGCTCCC CCCGGCTCCT TCACCATTCC CCAGTCTGTC
    CCAGATCGCC TGAGGATCCG TGTGAACAAA ATCAGTTTAC AAGACTATGA AGGACTCCAC
    TATGACAAGG ACAAACTCCG GGAAGCTTCC ATCCCTCTGG GTATTCTAGT TGTTCGTGGA
    GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGTC TACAGGAGGA CTTGCAGTCA
    GTTTCTTCTG GGTCACAGAG AGTTCATTAC CAGGACCAAG AAACCTCCTT CCCCAGGGCA
    CTCTCAGCTC AGAGGCTGTC CCCACGGTGG TGCTTCCTAG ATGCAACGTC TGCTGACCGC
    TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCTCACGAT
    GAAATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC AGGCAGGGAC CCCTCCAGGA
    ATGCCTGACC TGGTGGTTGA ACAGGCTTCT GGGCTGTCTG ACTGGTGGAA CCCTGCCCTG
    AGGAAACGTA TGCTGAGTGA CAGTGGGATG ATCACACCCC ACTATGAGGA CTCAGATCTG
    AAAGACTTCA GCCACTCCAG AGTGCTACAG TCCCCAGTCT CTTCAGAAGA TCATGCAATT
    TTGCAGGCAG TCCTCACCGG TGACCTTATG AAGCTCATGG AGAGCTATAA GAATGGAGGG
    AGCTTACTAA TTCAGGGACC AGGGCACTGT TCTCTCCTTC ACTACGCAGC TAAGACCGGC
    AATGGGGACA TTGTGAAGTA CATCCTTGAC CACGGACCTG CAGAATTACT GGATATGGCA
    GACAGTGAAA CGGGCGAGAC TGCACTGCAC AAGGCTGCCT GCCAAAGGAA CCGGGCTGTA
    TGCCAGCTTC TGGTGGATGC AGGAGCATCT CTGAGACAGA CAGACTCCAA GGGCAAGACA
    CCCCAGGAAC GAGCACAGCA GGCCGGGGAC CCAGATTTGG CTGCTTACCT AGAAAGCCGA
    CAGAACTATA AGATCATTGG CCATGAAGAC CTGGAAACTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177120.1
    CDS16489..19476
    Translation

    Target ORF information:

    RefSeq Version XM_017321631.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321631.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGATAC CACACTTAGG ACTGAGTTTT CCAAGGAAAG CCATCTCCCG GACAGGCCTC 
    CAGCATCTGG CACCTGCACA TCCTCTTGGT CTTCCTGTGG CTAACGGTCC AGCGAAGGAG
    CCCAGAGCCA CTCTGGACTG GAGCGAGAAT GCTGTGAATG GGGAGCACCT GTGGCTGGAG
    ACCAACGTTT CAGGGGACCT CTGCTACCTG GGGGAGGAGA ACTGCCAAGT CCGATTTGCA
    AAATCCGCTC TCAGGAGGAA GTGTGCCGTC TGCAAAATCG TGGTCCACAC AGCCTGCATT
    GAGCAACTAG AGAAGATTAA TTTCAGATGT AAACCAACAT TTCGGGAAGG AGGCTCCAGA
    TCGCCAAGAG AAAACTTTGT GCGTCACCAC TGGGTCCACA GGAGGCGGCA GGAGGGAAAA
    TGTAAGCAGT GTGGTAAGGG CTTCCAGCAA AAGTTCTCCT TCCACAGTAA AGAGATCGTG
    GCCATCAGCT GTTCTTGGTG CAAGCAGGCG TTTCACAATA AAGTGACCTG CTTCATGCTG
    CATCATATTG AGGAACCCTG CTCCCTGGGG GCTCACGCCG CTGTTATTGT CCCGCCCACC
    TGGATCATTA AGGTGAAGAA ACCTCAGGTG ATCTCCATAC CAGGAGATGA ACTTGAACTT
    GGTTGGGCGG GAGACGGACC TGGTTGGGCG GGAGACTGGA GCCCCTCAGA GAATTTGGGA
    TTCTTCCATT TTTACTGGGA GGGTTGGCAG AACTCCCTGA AGGCTTCCAA CCGGAAGAAG
    AAGAGGACAA GTTTTAAAAG GAAAGCCAGT AAAAGAGGAA CTGAGCAGGA AACCAAAGGC
    CGTCCTTTTG TGATCAAGCC CATCTCCTCC CCTCTCATGA AGCCTTTGCT TGTCTTTGTG
    AACCCAAAGA GTGGGGGAAA CCAGGGAACC AAAGTCCTAC AGATGTTTAT GTGGTACCTG
    AATCCACGGC AAGTCTTTGA TCTTTCTCAG GAAGGGCCAA AAGATGCGCT GGAGATGTAC
    AGGAAGGTAC CAAATCTGAG AATTCTGGCC TGCGGTGGGG ATGGAACGGT GGGCTGGATC
    CTCTCCATCC TGGACGAGCT GCAGCTGAGC CCACAGCCTC CTGTGGGGGT GCTTCCACTG
    GGGACTGGGA ATGACCTGGC TCGGACGCTC AACTGGGGAG GGGGTTACAC CGATGAGCCT
    GTTTCTAAGA TCCTGTGCCA GGTGGAAGAT GGGACCATTG TACAGTTGGA TCGCTGGAAC
    CTCCACGTAG AGAGAAACCC AGACCTGCCG CCCGAAGAAC TTGAAGATGG CGTGTGTAAG
    CTTCCTCTGA ATGTCTTCAA TAATTACTTC AGCCTTGGAT TTGATGCTCA CGTCACATTG
    GAGTTCCATG AATCCCGAGA AGCAAATCCA GAGAAATTCA ATAGTCGTTT TCGAAATAAA
    ATGTTCTATG CAGGGGCAGC CTTTTCTGAT TTCCTACAAA GAAGTTCTAG AGATCTATCC
    AAACACGTCA AAGTTGTCTG TGATGGGACA GACCTCACCC CAAAGATCCA GGACCTGAAG
    TTCCAGTGTA TAGTATTTTT AAATATTCCC AGATATTGTG CTGGCACAAT GCCTTGGGGA
    AACCCTGGAG ATCATCATGA CTTTGAACCC CAACGTCATG ATGACGGCTA CATTGAAGTC
    ATTGGATTCA CTATGGCCTC TTTGGCTGCC CTGCAAGTTG GGGGGCACGG AGAAAGGCTG
    CACCAGTGTC GGGAAGTGAT GCTTCTGACT TACAAGTCCA TTCCCATGCA AGTGGACGGG
    GAGCCATGTC GGTTGGCACC TGCTATGATT CGGATCTCCT TGAGGAATCA GGCCAACATG
    GTACAGAAGA GCAAGCGGAG AACCTCGATG CCTTTGCTCA ATGACATCCA TCAGGTGCAA
    GCTGCGGACC TGCGGCGAGT GTCTGCTCCC CCCGGCTCCT TCACCATTCC CCAGTCTGTC
    CCAGATCGCC TGAGGATCCG TGTGAACAAA ATCAGTTTAC AAGACTATGA AGGACTCCAC
    TATGACAAGG ACAAACTCCG GGAAGCTTCC ATCCCTCTGG GTATTCTAGT TGTTCGTGGA
    GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGTC TACAGGAGGA CTTGCAGTCA
    GTTTCTTCTG GGTCACAGAG AGTTCATTAC CAGGACCAAG AAACCTCCTT CCCCAGGGCA
    CTCTCAGCTC AGAGGCTGTC CCCACGGTGG TGCTTCCTAG ATGCAACGTC TGCTGACCGC
    TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCTCACGAT
    GAAATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC AGGCAGGGAC CCCTCCAGGA
    ATGCCTGACC TGGTGGTTGA ACAGGCTTCT GGGCTGTCTG ACTGGTGGAA CCCTGCCCTG
    AGGAAACGTA TGCTGAGTGA CAGTGGGATG ATCACACCCC ACTATGAGGA CTCAGATCTG
    AAAGACTTCA GCCACTCCAG AGTGCTACAG TCCCCAGTCT CTTCAGAAGA TCATGCAATT
    TTGCAGGCAG TCCTCACCGG TGACCTTATG AAGCTCATGG AGAGCTATAA GAATGGAGGG
    AGCTTACTAA TTCAGGGACC AGGGCACTGT TCTCTCCTTC ACTACGCAGC TAAGACCGGC
    AATGGGGACA TTGTGAAGTA CATCCTTGAC CACGGACCTG CAGAATTACT GGATATGGCA
    GACAGTGAAA CGGGCGAGAC TGCACTGCAC AAGGCTGCCT GCCAAAGGAA CCGGGCTGTA
    TGCCAGCTTC TGGTGGATGC AGGAGCATCT CTGAGACAGA CAGACTCCAA GGGCAAGACA
    CCCCAGGAAC GAGCACAGCA GGCCGGGGAC CCAGATTTGG CTGCTTACCT AGAAAGCCGA
    CAGAACTATA AGATCATTGG CCATGAAGAC CTGGAAACTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81756
    Clone ID Related Accession (Same CDS sequence) XM_017321632.1 , XM_017321632.2
    Accession Version XM_017321632.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGATCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACC AAGAAACCTC CTTCCCCAGG
    GCACTCTCAG CTCAGAGGCT GTCCCCACGG TGGTGCTTCC TAGATGCAAC GTCTGCTGAC
    CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCTCAC
    GATGAAATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCAGGCAGG GACCCCTCCA
    GGAATGCCTG ACCTGGTGGT TGAACAGGCT TCTGGGCTGT CTGACTGGTG GAACCCTGCC
    CTGAGGAAAC GTATGCTGAG TGACAGTGGG ATGATCACAC CCCACTATGA GGACTCAGAT
    CTGAAAGACT TCAGCCACTC CAGAGTGCTA CAGTGTGATC ATGGTTGGAA AGCAACATGC
    TGCTTGGAAT TTTGCAGAAG AACTGATACT TTGGGCTATG CGAAATCATC TTATCTGCCC
    ATATTTTGCT ACAGGAACAC GTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177121.1
    CDS1008..3914
    Translation

    Target ORF information:

    RefSeq Version XM_017321632.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321632.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGATCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACC AAGAAACCTC CTTCCCCAGG
    GCACTCTCAG CTCAGAGGCT GTCCCCACGG TGGTGCTTCC TAGATGCAAC GTCTGCTGAC
    CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCTCAC
    GATGAAATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCAGGCAGG GACCCCTCCA
    GGAATGCCTG ACCTGGTGGT TGAACAGGCT TCTGGGCTGT CTGACTGGTG GAACCCTGCC
    CTGAGGAAAC GTATGCTGAG TGACAGTGGG ATGATCACAC CCCACTATGA GGACTCAGAT
    CTGAAAGACT TCAGCCACTC CAGAGTGCTA CAGTGTGATC ATGGTTGGAA AGCAACATGC
    TGCTTGGAAT TTTGCAGAAG AACTGATACT TTGGGCTATG CGAAATCATC TTATCTGCCC
    ATATTTTGCT ACAGGAACAC GTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81757
    Clone ID Related Accession (Same CDS sequence) XM_017321633.2 , XM_017321633.1
    Accession Version XM_017321633.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321633.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT TATCTTGGAT GAGACTCTCA GACTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177122.1
    CDS1008..2906
    Translation

    Target ORF information:

    RefSeq Version XM_017321633.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321633.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT TATCTTGGAT GAGACTCTCA GACTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu89227
    Clone ID Related Accession (Same CDS sequence) XM_030255454.1
    Accession Version XM_030255454.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2832bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGAACTCCC TGAAGGCTTC CAACCGGAAG AAGAAGAGGA CAAGTTTTAA AAGGAAAGCC
    AGTAAAAGAG GAACTGAGCA GGAAACCAAA GGCCGTCCTT TTGTGATCAA GCCCATCTCC
    TCCCCTCTCA TGAAGCCTTT GCTTGTCTTT GTGAACCCAA AGAGTGGGGG AAACCAGGGA
    ACCAAAGTCC TACAGATGTT TATGTGGTAC CTGAATCCAC GGCAAGTCTT TGATCTTTCT
    CAGGAAGGGC CAAAAGATGC GCTGGAGATG TACAGGAAGG TACCAAATCT GAGAATTCTG
    GCCTGCGGTG GGGATGGAAC GGTGGGCTGG ATCCTCTCCA TCCTGGACGA GCTGCAGCTG
    AGCCCACAGC CTCCTGTGGG GGTGCTTCCA CTGGGGACTG GGAATGACCT GGCTCGGACG
    CTCAACTGGG GAGGGGGTTA CACCGATGAG CCTGTTTCTA AGATCCTGTG CCAGGTGGAA
    GATGGGACCA TTGTACAGTT GGATCGCTGG AACCTCCACG TAGAGAGAAA CCCAGACCTG
    CCGCCCGAAG AACTTGAAGA TGGCGTGTGT AAGCTTCCTC TGAATGTCTT CAATAATTAC
    TTCAGCCTTG GATTTGATGC TCACGTCACA TTGGAGTTCC ATGAATCCCG AGAAGCAAAT
    CCAGAGAAAT TCAATAGTCG TTTTCGAAAT AAAATGTTCT ATGCAGGGGC AGCCTTTTCT
    GATTTCCTAC AAAGAAGTTC TAGAGATCTA TCCAAACACG TCAAAGTTGT CTGTGATGGG
    ACAGACCTCA CCCCAAAGAT CCAGGACCTG AAGTTCCAGT GTATAGTATT TTTAAATATT
    CCCAGATATT GTGCTGGCAC AATGCCTTGG GGAAACCCTG GAGATCATCA TGACTTTGAA
    CCCCAACGTC ATGATGACGG CTACATTGAA GTCATTGGAT TCACTATGGC CTCTTTGGCT
    GCCCTGCAAG TTGGGGGGCA CGGAGAAAGG CTGCACCAGT GTCGGGAAGT GATGCTTCTG
    ACTTACAAGT CCATTCCCAT GCAAGTGGAC GGGGAGCCAT GTCGGTTGGC ACCTGCTATG
    ATTCGGATCT CCTTGAGGAA TCAGGCCAAC ATGGTACAGA AGAGCAAGCG GAGAACCTCG
    ATGCCTTTGC TCAATGACAT CCATCAGGTG CAAGCTGCGG ACCTGCGGCG AGTGTCTGCT
    CCCCCCGGCT CCTTCACCAT TCCCCAGTCT GTCCCAGATC GCCTGAGGAT CCGTGTGAAC
    AAAATCAGTT TACAAGACTA TGAAGGACTC CACTATGACA AGGACAAACT CCGGGAAGCT
    TCCATCCCTC TGGGTATTCT AGTTGTTCGT GGAGACTGTG ATTTGGAGAC TTGCCGTATG
    TACATAGACC GTCTACAGGA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTG TGATCATGGT TGGAAAGCAA CATGCTGCTT GGAATTTTGC
    AGAAGAACTG ATACTTTGGG CTATGCGAAA TCATCTTATC TGCCCATATT TTGCTACAGG
    AACACGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030111314.1
    CDS1008..3839
    Translation

    Target ORF information:

    RefSeq Version XM_030255454.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030255454.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGAACTCCC TGAAGGCTTC CAACCGGAAG AAGAAGAGGA CAAGTTTTAA AAGGAAAGCC
    AGTAAAAGAG GAACTGAGCA GGAAACCAAA GGCCGTCCTT TTGTGATCAA GCCCATCTCC
    TCCCCTCTCA TGAAGCCTTT GCTTGTCTTT GTGAACCCAA AGAGTGGGGG AAACCAGGGA
    ACCAAAGTCC TACAGATGTT TATGTGGTAC CTGAATCCAC GGCAAGTCTT TGATCTTTCT
    CAGGAAGGGC CAAAAGATGC GCTGGAGATG TACAGGAAGG TACCAAATCT GAGAATTCTG
    GCCTGCGGTG GGGATGGAAC GGTGGGCTGG ATCCTCTCCA TCCTGGACGA GCTGCAGCTG
    AGCCCACAGC CTCCTGTGGG GGTGCTTCCA CTGGGGACTG GGAATGACCT GGCTCGGACG
    CTCAACTGGG GAGGGGGTTA CACCGATGAG CCTGTTTCTA AGATCCTGTG CCAGGTGGAA
    GATGGGACCA TTGTACAGTT GGATCGCTGG AACCTCCACG TAGAGAGAAA CCCAGACCTG
    CCGCCCGAAG AACTTGAAGA TGGCGTGTGT AAGCTTCCTC TGAATGTCTT CAATAATTAC
    TTCAGCCTTG GATTTGATGC TCACGTCACA TTGGAGTTCC ATGAATCCCG AGAAGCAAAT
    CCAGAGAAAT TCAATAGTCG TTTTCGAAAT AAAATGTTCT ATGCAGGGGC AGCCTTTTCT
    GATTTCCTAC AAAGAAGTTC TAGAGATCTA TCCAAACACG TCAAAGTTGT CTGTGATGGG
    ACAGACCTCA CCCCAAAGAT CCAGGACCTG AAGTTCCAGT GTATAGTATT TTTAAATATT
    CCCAGATATT GTGCTGGCAC AATGCCTTGG GGAAACCCTG GAGATCATCA TGACTTTGAA
    CCCCAACGTC ATGATGACGG CTACATTGAA GTCATTGGAT TCACTATGGC CTCTTTGGCT
    GCCCTGCAAG TTGGGGGGCA CGGAGAAAGG CTGCACCAGT GTCGGGAAGT GATGCTTCTG
    ACTTACAAGT CCATTCCCAT GCAAGTGGAC GGGGAGCCAT GTCGGTTGGC ACCTGCTATG
    ATTCGGATCT CCTTGAGGAA TCAGGCCAAC ATGGTACAGA AGAGCAAGCG GAGAACCTCG
    ATGCCTTTGC TCAATGACAT CCATCAGGTG CAAGCTGCGG ACCTGCGGCG AGTGTCTGCT
    CCCCCCGGCT CCTTCACCAT TCCCCAGTCT GTCCCAGATC GCCTGAGGAT CCGTGTGAAC
    AAAATCAGTT TACAAGACTA TGAAGGACTC CACTATGACA AGGACAAACT CCGGGAAGCT
    TCCATCCCTC TGGGTATTCT AGTTGTTCGT GGAGACTGTG ATTTGGAGAC TTGCCGTATG
    TACATAGACC GTCTACAGGA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTG TGATCATGGT TGGAAAGCAA CATGCTGCTT GGAATTTTGC
    AGAAGAACTG ATACTTTGGG CTATGCGAAA TCATCTTATC TGCCCATATT TTGCTACAGG
    AACACGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37360
    Clone ID Related Accession (Same CDS sequence) XM_006506257.4 , XM_006506257.3
    Accession Version XM_006506257.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506257.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGC TCTCACCACT GGGTCAAACA TCTATTATAT GAACTCCCTG
    AAGGCTTCCA ACCGGAAGAA GAAGAGGACA AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA
    ACTGAGCAGG AAACCAAAGG CCGTCCTTTT GTGATCAAGC CCATCTCCTC CCCTCTCATG
    AAGCCTTTGC TTGTCTTTGT GAACCCAAAG AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA
    CAGATGTTTA TGTGGTACCT GAATCCACGG CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA
    AAAGATGCGC TGGAGATGTA CAGGAAGGTA CCAAATCTGA GAATTCTGGC CTGCGGTGGG
    GATGGAACGG TGGGCTGGAT CCTCTCCATC CTGGACGAGC TGCAGCTGAG CCCACAGCCT
    CCTGTGGGGG TGCTTCCACT GGGGACTGGG AATGACCTGG CTCGGACGCT CAACTGGGGA
    GGGGGTTACA CCGATGAGCC TGTTTCTAAG ATCCTGTGCC AGGTGGAAGA TGGGACCATT
    GTACAGTTGG ATCGCTGGAA CCTCCACGTA GAGAGAAACC CAGACCTGCC GCCCGAAGAA
    CTTGAAGATG GCGTGTGTAA GCTTCCTCTG AATGTCTTCA ATAATTACTT CAGCCTTGGA
    TTTGATGCTC ACGTCACATT GGAGTTCCAT GAATCCCGAG AAGCAAATCC AGAGAAATTC
    AATAGTCGTT TTCGAAATAA AATGTTCTAT GCAGGGGCAG CCTTTTCTGA TTTCCTACAA
    AGAAGTTCTA GAGATCTATC CAAACACGTC AAAGTTGTCT GTGATGGGAC AGACCTCACC
    CCAAAGATCC AGGACCTGAA GTTCCAGTGT ATAGTATTTT TAAATATTCC CAGATATTGT
    GCTGGCACAA TGCCTTGGGG AAACCCTGGA GATCATCATG ACTTTGAACC CCAACGTCAT
    GATGACGGCT ACATTGAAGT CATTGGATTC ACTATGGCCT CTTTGGCTGC CCTGCAAGTT
    GGGGGGCACG GAGAAAGGCT GCACCAGTGT CGGGAAGTGA TGCTTCTGAC TTACAAGTCC
    ATTCCCATGC AAGTGGACGG GGAGCCATGT CGGTTGGCAC CTGCTATGAT TCGGATCTCC
    TTGAGGAATC AGGCCAACAT GGTACAGAAG AGCAAGCGGA GAACCTCGAT GCCTTTGCTC
    AATGACATCC ATCAGGTGCA AGCTGCGGAC CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC
    TTCACCATTC CCCAGTCTGT CCCAGATCGC CTGAGGATCC GTGTGAACAA AATCAGTTTA
    CAAGACTATG AAGGACTCCA CTATGACAAG GACAAACTCC GGGAAGCTTC CATCCCTCTG
    GGTATTCTAG TTGTTCGTGG AGACTGTGAT TTGGAGACTT GCCGTATGTA CATAGACCGT
    CTACAGGAGG ACTTGCAGTC AGTTTCTTCT GGGTCACAGA GAGTTCATTA CCAGGACCAA
    GAAACCTCCT TCCCCAGGGC ACTCTCAGCT CAGAGGCTGT CCCCACGGTG GTGCTTCCTA
    GATGCAACGT CTGCTGACCG CTTTTATCGA ATAGACAGAT CTCAGGAACA TTTGCACTTT
    GTGATGGAGA TTTCTCACGA TGAAATTTTT ATTCTGGACC CAGATATGGT GGTGTCACAG
    CAGGCAGGGA CCCCTCCAGG AATGCCTGAC CTGGTGGTTG AACAGGCTTC TGGGCTGTCT
    GACTGGTGGA ACCCTGCCCT GAGGAAACGT ATGCTGAGTG ACAGTGGGAT GATCACACCC
    CACTATGAGG ACTCAGATCT GAAAGACTTC AGCCACTCCA GAGTGCTACA GTCCCCAGTC
    TCTTCAGAAG ATCATGCAAT TTTGCAGGCA GTCCTCACCG GTGACCTTAT GAAGCTCATG
    GAGAGCTATA AGAATGGAGG GAGCTTACTA ATTCAGGGAC CAGGGCACTG TTCTCTCCTT
    CACTACGCAG CTAAGACCGG CAATGGGGAC ATTGTGAAGT ACATCCTTGA CCACGGACCT
    GCAGAATTAC TGGATATGGC AGACAGTGAA ACGGGCGAGA CTGCACTGCA CAAGGCTGCC
    TGCCAAAGGA ACCGGGCTGT ATGCCAGCTT CTGGTGGATG CAGGAGCATC TCTGAGACAG
    ACAGACTCCA AGGGCAAGAC ACCCCAGGAA CGAGCACAGC AGGCCGGGGA CCCAGATTTG
    GCTGCTTACC TAGAAAGCCG ACAGAACTAT AAGATCATTG GCCATGAAGA CCTGGAAACT
    GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506320.1
    CDS1008..4256
    Translation

    Target ORF information:

    RefSeq Version XM_006506257.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506257.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGC TCTCACCACT GGGTCAAACA TCTATTATAT GAACTCCCTG
    AAGGCTTCCA ACCGGAAGAA GAAGAGGACA AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA
    ACTGAGCAGG AAACCAAAGG CCGTCCTTTT GTGATCAAGC CCATCTCCTC CCCTCTCATG
    AAGCCTTTGC TTGTCTTTGT GAACCCAAAG AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA
    CAGATGTTTA TGTGGTACCT GAATCCACGG CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA
    AAAGATGCGC TGGAGATGTA CAGGAAGGTA CCAAATCTGA GAATTCTGGC CTGCGGTGGG
    GATGGAACGG TGGGCTGGAT CCTCTCCATC CTGGACGAGC TGCAGCTGAG CCCACAGCCT
    CCTGTGGGGG TGCTTCCACT GGGGACTGGG AATGACCTGG CTCGGACGCT CAACTGGGGA
    GGGGGTTACA CCGATGAGCC TGTTTCTAAG ATCCTGTGCC AGGTGGAAGA TGGGACCATT
    GTACAGTTGG ATCGCTGGAA CCTCCACGTA GAGAGAAACC CAGACCTGCC GCCCGAAGAA
    CTTGAAGATG GCGTGTGTAA GCTTCCTCTG AATGTCTTCA ATAATTACTT CAGCCTTGGA
    TTTGATGCTC ACGTCACATT GGAGTTCCAT GAATCCCGAG AAGCAAATCC AGAGAAATTC
    AATAGTCGTT TTCGAAATAA AATGTTCTAT GCAGGGGCAG CCTTTTCTGA TTTCCTACAA
    AGAAGTTCTA GAGATCTATC CAAACACGTC AAAGTTGTCT GTGATGGGAC AGACCTCACC
    CCAAAGATCC AGGACCTGAA GTTCCAGTGT ATAGTATTTT TAAATATTCC CAGATATTGT
    GCTGGCACAA TGCCTTGGGG AAACCCTGGA GATCATCATG ACTTTGAACC CCAACGTCAT
    GATGACGGCT ACATTGAAGT CATTGGATTC ACTATGGCCT CTTTGGCTGC CCTGCAAGTT
    GGGGGGCACG GAGAAAGGCT GCACCAGTGT CGGGAAGTGA TGCTTCTGAC TTACAAGTCC
    ATTCCCATGC AAGTGGACGG GGAGCCATGT CGGTTGGCAC CTGCTATGAT TCGGATCTCC
    TTGAGGAATC AGGCCAACAT GGTACAGAAG AGCAAGCGGA GAACCTCGAT GCCTTTGCTC
    AATGACATCC ATCAGGTGCA AGCTGCGGAC CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC
    TTCACCATTC CCCAGTCTGT CCCAGATCGC CTGAGGATCC GTGTGAACAA AATCAGTTTA
    CAAGACTATG AAGGACTCCA CTATGACAAG GACAAACTCC GGGAAGCTTC CATCCCTCTG
    GGTATTCTAG TTGTTCGTGG AGACTGTGAT TTGGAGACTT GCCGTATGTA CATAGACCGT
    CTACAGGAGG ACTTGCAGTC AGTTTCTTCT GGGTCACAGA GAGTTCATTA CCAGGACCAA
    GAAACCTCCT TCCCCAGGGC ACTCTCAGCT CAGAGGCTGT CCCCACGGTG GTGCTTCCTA
    GATGCAACGT CTGCTGACCG CTTTTATCGA ATAGACAGAT CTCAGGAACA TTTGCACTTT
    GTGATGGAGA TTTCTCACGA TGAAATTTTT ATTCTGGACC CAGATATGGT GGTGTCACAG
    CAGGCAGGGA CCCCTCCAGG AATGCCTGAC CTGGTGGTTG AACAGGCTTC TGGGCTGTCT
    GACTGGTGGA ACCCTGCCCT GAGGAAACGT ATGCTGAGTG ACAGTGGGAT GATCACACCC
    CACTATGAGG ACTCAGATCT GAAAGACTTC AGCCACTCCA GAGTGCTACA GTCCCCAGTC
    TCTTCAGAAG ATCATGCAAT TTTGCAGGCA GTCCTCACCG GTGACCTTAT GAAGCTCATG
    GAGAGCTATA AGAATGGAGG GAGCTTACTA ATTCAGGGAC CAGGGCACTG TTCTCTCCTT
    CACTACGCAG CTAAGACCGG CAATGGGGAC ATTGTGAAGT ACATCCTTGA CCACGGACCT
    GCAGAATTAC TGGATATGGC AGACAGTGAA ACGGGCGAGA CTGCACTGCA CAAGGCTGCC
    TGCCAAAGGA ACCGGGCTGT ATGCCAGCTT CTGGTGGATG CAGGAGCATC TCTGAGACAG
    ACAGACTCCA AGGGCAAGAC ACCCCAGGAA CGAGCACAGC AGGCCGGGGA CCCAGATTTG
    GCTGCTTACC TAGAAAGCCG ACAGAACTAT AAGATCATTG GCCATGAAGA CCTGGAAACT
    GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37361
    Clone ID Related Accession (Same CDS sequence) XM_006506258.4 , XM_006506258.3
    Accession Version XM_006506258.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506258.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGGAAACCA AAGGCCGTCC TTTTGTGATC
    AAGCCCATCT CCTCCCCTCT CATGAAGCCT TTGCTTGTCT TTGTGAACCC AAAGAGTGGG
    GGAAACCAGG GAACCAAAGT CCTACAGATG TTTATGTGGT ACCTGAATCC ACGGCAAGTC
    TTTGATCTTT CTCAGGAAGG GCCAAAAGAT GCGCTGGAGA TGTACAGGAA GGTACCAAAT
    CTGAGAATTC TGGCCTGCGG TGGGGATGGA ACGGTGGGCT GGATCCTCTC CATCCTGGAC
    GAGCTGCAGC TGAGCCCACA GCCTCCTGTG GGGGTGCTTC CACTGGGGAC TGGGAATGAC
    CTGGCTCGGA CGCTCAACTG GGGAGGGGGT TACACCGATG AGCCTGTTTC TAAGATCCTG
    TGCCAGGTGG AAGATGGGAC CATTGTACAG TTGGATCGCT GGAACCTCCA CGTAGAGAGA
    AACCCAGACC TGCCGCCCGA AGAACTTGAA GATGGCGTGT GTAAGCTTCC TCTGAATGTC
    TTCAATAATT ACTTCAGCCT TGGATTTGAT GCTCACGTCA CATTGGAGTT CCATGAATCC
    CGAGAAGCAA ATCCAGAGAA ATTCAATAGT CGTTTTCGAA ATAAAATGTT CTATGCAGGG
    GCAGCCTTTT CTGATTTCCT ACAAAGAAGT TCTAGAGATC TATCCAAACA CGTCAAAGTT
    GTCTGTGATG GGACAGACCT CACCCCAAAG ATCCAGGACC TGAAGTTCCA GTGTATAGTA
    TTTTTAAATA TTCCCAGATA TTGTGCTGGC ACAATGCCTT GGGGAAACCC TGGAGATCAT
    CATGACTTTG AACCCCAACG TCATGATGAC GGCTACATTG AAGTCATTGG ATTCACTATG
    GCCTCTTTGG CTGCCCTGCA AGTTGGGGGG CACGGAGAAA GGCTGCACCA GTGTCGGGAA
    GTGATGCTTC TGACTTACAA GTCCATTCCC ATGCAAGTGG ACGGGGAGCC ATGTCGGTTG
    GCACCTGCTA TGATTCGGAT CTCCTTGAGG AATCAGGCCA ACATGGTACA GAAGAGCAAG
    CGGAGAACCT CGATGCCTTT GCTCAATGAC ATCCATCAGG TGCAAGCTGC GGACCTGCGG
    CGAGTGTCTG CTCCCCCCGG CTCCTTCACC ATTCCCCAGT CTGTCCCAGA TCGCCTGAGG
    ATCCGTGTGA ACAAAATCAG TTTACAAGAC TATGAAGGAC TCCACTATGA CAAGGACAAA
    CTCCGGGAAG CTTCCATCCC TCTGGGTATT CTAGTTGTTC GTGGAGACTG TGATTTGGAG
    ACTTGCCGTA TGTACATAGA CCGTCTACAG GAGGACTTGC AGTCAGTTTC TTCTGGGTCA
    CAGAGAGTTC ATTACCAGGA CCAAGAAACC TCCTTCCCCA GGGCACTCTC AGCTCAGAGG
    CTGTCCCCAC GGTGGTGCTT CCTAGATGCA ACGTCTGCTG ACCGCTTTTA TCGAATAGAC
    AGATCTCAGG AACATTTGCA CTTTGTGATG GAGATTTCTC ACGATGAAAT TTTTATTCTG
    GACCCAGATA TGGTGGTGTC ACAGCAGGCA GGGACCCCTC CAGGAATGCC TGACCTGGTG
    GTTGAACAGG CTTCTGGGCT GTCTGACTGG TGGAACCCTG CCCTGAGGAA ACGTATGCTG
    AGTGACAGTG GGATGATCAC ACCCCACTAT GAGGACTCAG ATCTGAAAGA CTTCAGCCAC
    TCCAGAGTGC TACAGTCCCC AGTCTCTTCA GAAGATCATG CAATTTTGCA GGCAGTCCTC
    ACCGGTGACC TTATGAAGCT CATGGAGAGC TATAAGAATG GAGGGAGCTT ACTAATTCAG
    GGACCAGGGC ACTGTTCTCT CCTTCACTAC GCAGCTAAGA CCGGCAATGG GGACATTGTG
    AAGTACATCC TTGACCACGG ACCTGCAGAA TTACTGGATA TGGCAGACAG TGAAACGGGC
    GAGACTGCAC TGCACAAGGC TGCCTGCCAA AGGAACCGGG CTGTATGCCA GCTTCTGGTG
    GATGCAGGAG CATCTCTGAG ACAGACAGAC TCCAAGGGCA AGACACCCCA GGAACGAGCA
    CAGCAGGCCG GGGACCCAGA TTTGGCTGCT TACCTAGAAA GCCGACAGAA CTATAAGATC
    ATTGGCCATG AAGACCTGGA AACTGCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506321.1
    CDS1008..4220
    Translation

    Target ORF information:

    RefSeq Version XM_006506258.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506258.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGGAAACCA AAGGCCGTCC TTTTGTGATC
    AAGCCCATCT CCTCCCCTCT CATGAAGCCT TTGCTTGTCT TTGTGAACCC AAAGAGTGGG
    GGAAACCAGG GAACCAAAGT CCTACAGATG TTTATGTGGT ACCTGAATCC ACGGCAAGTC
    TTTGATCTTT CTCAGGAAGG GCCAAAAGAT GCGCTGGAGA TGTACAGGAA GGTACCAAAT
    CTGAGAATTC TGGCCTGCGG TGGGGATGGA ACGGTGGGCT GGATCCTCTC CATCCTGGAC
    GAGCTGCAGC TGAGCCCACA GCCTCCTGTG GGGGTGCTTC CACTGGGGAC TGGGAATGAC
    CTGGCTCGGA CGCTCAACTG GGGAGGGGGT TACACCGATG AGCCTGTTTC TAAGATCCTG
    TGCCAGGTGG AAGATGGGAC CATTGTACAG TTGGATCGCT GGAACCTCCA CGTAGAGAGA
    AACCCAGACC TGCCGCCCGA AGAACTTGAA GATGGCGTGT GTAAGCTTCC TCTGAATGTC
    TTCAATAATT ACTTCAGCCT TGGATTTGAT GCTCACGTCA CATTGGAGTT CCATGAATCC
    CGAGAAGCAA ATCCAGAGAA ATTCAATAGT CGTTTTCGAA ATAAAATGTT CTATGCAGGG
    GCAGCCTTTT CTGATTTCCT ACAAAGAAGT TCTAGAGATC TATCCAAACA CGTCAAAGTT
    GTCTGTGATG GGACAGACCT CACCCCAAAG ATCCAGGACC TGAAGTTCCA GTGTATAGTA
    TTTTTAAATA TTCCCAGATA TTGTGCTGGC ACAATGCCTT GGGGAAACCC TGGAGATCAT
    CATGACTTTG AACCCCAACG TCATGATGAC GGCTACATTG AAGTCATTGG ATTCACTATG
    GCCTCTTTGG CTGCCCTGCA AGTTGGGGGG CACGGAGAAA GGCTGCACCA GTGTCGGGAA
    GTGATGCTTC TGACTTACAA GTCCATTCCC ATGCAAGTGG ACGGGGAGCC ATGTCGGTTG
    GCACCTGCTA TGATTCGGAT CTCCTTGAGG AATCAGGCCA ACATGGTACA GAAGAGCAAG
    CGGAGAACCT CGATGCCTTT GCTCAATGAC ATCCATCAGG TGCAAGCTGC GGACCTGCGG
    CGAGTGTCTG CTCCCCCCGG CTCCTTCACC ATTCCCCAGT CTGTCCCAGA TCGCCTGAGG
    ATCCGTGTGA ACAAAATCAG TTTACAAGAC TATGAAGGAC TCCACTATGA CAAGGACAAA
    CTCCGGGAAG CTTCCATCCC TCTGGGTATT CTAGTTGTTC GTGGAGACTG TGATTTGGAG
    ACTTGCCGTA TGTACATAGA CCGTCTACAG GAGGACTTGC AGTCAGTTTC TTCTGGGTCA
    CAGAGAGTTC ATTACCAGGA CCAAGAAACC TCCTTCCCCA GGGCACTCTC AGCTCAGAGG
    CTGTCCCCAC GGTGGTGCTT CCTAGATGCA ACGTCTGCTG ACCGCTTTTA TCGAATAGAC
    AGATCTCAGG AACATTTGCA CTTTGTGATG GAGATTTCTC ACGATGAAAT TTTTATTCTG
    GACCCAGATA TGGTGGTGTC ACAGCAGGCA GGGACCCCTC CAGGAATGCC TGACCTGGTG
    GTTGAACAGG CTTCTGGGCT GTCTGACTGG TGGAACCCTG CCCTGAGGAA ACGTATGCTG
    AGTGACAGTG GGATGATCAC ACCCCACTAT GAGGACTCAG ATCTGAAAGA CTTCAGCCAC
    TCCAGAGTGC TACAGTCCCC AGTCTCTTCA GAAGATCATG CAATTTTGCA GGCAGTCCTC
    ACCGGTGACC TTATGAAGCT CATGGAGAGC TATAAGAATG GAGGGAGCTT ACTAATTCAG
    GGACCAGGGC ACTGTTCTCT CCTTCACTAC GCAGCTAAGA CCGGCAATGG GGACATTGTG
    AAGTACATCC TTGACCACGG ACCTGCAGAA TTACTGGATA TGGCAGACAG TGAAACGGGC
    GAGACTGCAC TGCACAAGGC TGCCTGCCAA AGGAACCGGG CTGTATGCCA GCTTCTGGTG
    GATGCAGGAG CATCTCTGAG ACAGACAGAC TCCAAGGGCA AGACACCCCA GGAACGAGCA
    CAGCAGGCCG GGGACCCAGA TTTGGCTGCT TACCTAGAAA GCCGACAGAA CTATAAGATC
    ATTGGCCATG AAGACCTGGA AACTGCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37362
    Clone ID Related Accession (Same CDS sequence) XM_006506259.3 , XM_006506259.4
    Accession Version XM_006506259.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506259.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGAACTCCC TGAAGGCTTC CAACCGGAAG AAGAAGAGGA CAAGTTTTAA AAGGAAAGCC
    AGTAAAAGAG GAACTGAGCA GGAAACCAAA GGCCGTCCTT TTGTGATCAA GCCCATCTCC
    TCCCCTCTCA TGAAGCCTTT GCTTGTCTTT GTGAACCCAA AGAGTGGGGG AAACCAGGGA
    ACCAAAGTCC TACAGATGTT TATGTGGTAC CTGAATCCAC GGCAAGTCTT TGATCTTTCT
    CAGGAAGGGC CAAAAGATGC GCTGGAGATG TACAGGAAGG TACCAAATCT GAGAATTCTG
    GCCTGCGGTG GGGATGGAAC GGTGGGCTGG ATCCTCTCCA TCCTGGACGA GCTGCAGCTG
    AGCCCACAGC CTCCTGTGGG GGTGCTTCCA CTGGGGACTG GGAATGACCT GGCTCGGACG
    CTCAACTGGG GAGGGGGTTA CACCGATGAG CCTGTTTCTA AGATCCTGTG CCAGGTGGAA
    GATGGGACCA TTGTACAGTT GGATCGCTGG AACCTCCACG TAGAGAGAAA CCCAGACCTG
    CCGCCCGAAG AACTTGAAGA TGGCGTGTGT AAGCTTCCTC TGAATGTCTT CAATAATTAC
    TTCAGCCTTG GATTTGATGC TCACGTCACA TTGGAGTTCC ATGAATCCCG AGAAGCAAAT
    CCAGAGAAAT TCAATAGTCG TTTTCGAAAT AAAATGTTCT ATGCAGGGGC AGCCTTTTCT
    GATTTCCTAC AAAGAAGTTC TAGAGATCTA TCCAAACACG TCAAAGTTGT CTGTGATGGG
    ACAGACCTCA CCCCAAAGAT CCAGGACCTG AAGTTCCAGT GTATAGTATT TTTAAATATT
    CCCAGATATT GTGCTGGCAC AATGCCTTGG GGAAACCCTG GAGATCATCA TGACTTTGAA
    CCCCAACGTC ATGATGACGG CTACATTGAA GTCATTGGAT TCACTATGGC CTCTTTGGCT
    GCCCTGCAAG TTGGGGGGCA CGGAGAAAGG CTGCACCAGT GTCGGGAAGT GATGCTTCTG
    ACTTACAAGT CCATTCCCAT GCAAGTGGAC GGGGAGCCAT GTCGGTTGGC ACCTGCTATG
    ATTCGGATCT CCTTGAGGAA TCAGGCCAAC ATGGTACAGA AGAGCAAGCG GAGAACCTCG
    ATGCCTTTGC TCAATGACAT CCATCAGGTG CAAGCTGCGG ACCTGCGGCG AGTGTCTGCT
    CCCCCCGGCT CCTTCACCAT TCCCCAGTCT GTCCCAGATC GCCTGAGGAT CCGTGTGAAC
    AAAATCAGTT TACAAGACTA TGAAGGACTC CACTATGACA AGGACAAACT CCGGGAAGCT
    TCCATCCCTC TGGGTATTCT AGTTGTTCGT GGAGACTGTG ATTTGGAGAC TTGCCGTATG
    TACATAGACC GTCTACAGGA GGACTTGCAG TCAGTTTCTT CTGGGTCACA GAGAGTTCAT
    TACCAGGACC AAGAAACCTC CTTCCCCAGG GCACTCTCAG CTCAGAGGCT GTCCCCACGG
    TGGTGCTTCC TAGATGCAAC GTCTGCTGAC CGCTTTTATC GAATAGACAG ATCTCAGGAA
    CATTTGCACT TTGTGATGGA GATTTCTCAC GATGAAATTT TTATTCTGGA CCCAGATATG
    GTGGTGTCAC AGCAGGCAGG GACCCCTCCA GGAATGCCTG ACCTGGTGGT TGAACAGGCT
    TCTGGGCTGT CTGACTGGTG GAACCCTGCC CTGAGGAAAC GTATGCTGAG TGACAGTGGG
    ATGATCACAC CCCACTATGA GGACTCAGAT CTGAAAGACT TCAGCCACTC CAGAGTGCTA
    CAGTCCCCAG TCTCTTCAGA AGATCATGCA ATTTTGCAGG CAGTCCTCAC CGGTGACCTT
    ATGAAGCTCA TGGAGAGCTA TAAGAATGGA GGGAGCTTAC TAATTCAGGG ACCAGGGCAC
    TGTTCTCTCC TTCACTACGC AGCTAAGACC GGCAATGGGG ACATTGTGAA GTACATCCTT
    GACCACGGAC CTGCAGAATT ACTGGATATG GCAGACAGTG AAACGGGCGA GACTGCACTG
    CACAAGGCTG CCTGCCAAAG GAACCGGGCT GTATGCCAGC TTCTGGTGGA TGCAGGAGCA
    TCTCTGAGAC AGACAGACTC CAAGGGCAAG ACACCCCAGG AACGAGCACA GCAGGCCGGG
    GACCCAGATT TGGCTGCTTA CCTAGAAAGC CGACAGAACT ATAAGATCAT TGGCCATGAA
    GACCTGGAAA CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506322.1
    CDS1008..4208
    Translation

    Target ORF information:

    RefSeq Version XM_006506259.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506259.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGAACTCCC TGAAGGCTTC CAACCGGAAG AAGAAGAGGA CAAGTTTTAA AAGGAAAGCC
    AGTAAAAGAG GAACTGAGCA GGAAACCAAA GGCCGTCCTT TTGTGATCAA GCCCATCTCC
    TCCCCTCTCA TGAAGCCTTT GCTTGTCTTT GTGAACCCAA AGAGTGGGGG AAACCAGGGA
    ACCAAAGTCC TACAGATGTT TATGTGGTAC CTGAATCCAC GGCAAGTCTT TGATCTTTCT
    CAGGAAGGGC CAAAAGATGC GCTGGAGATG TACAGGAAGG TACCAAATCT GAGAATTCTG
    GCCTGCGGTG GGGATGGAAC GGTGGGCTGG ATCCTCTCCA TCCTGGACGA GCTGCAGCTG
    AGCCCACAGC CTCCTGTGGG GGTGCTTCCA CTGGGGACTG GGAATGACCT GGCTCGGACG
    CTCAACTGGG GAGGGGGTTA CACCGATGAG CCTGTTTCTA AGATCCTGTG CCAGGTGGAA
    GATGGGACCA TTGTACAGTT GGATCGCTGG AACCTCCACG TAGAGAGAAA CCCAGACCTG
    CCGCCCGAAG AACTTGAAGA TGGCGTGTGT AAGCTTCCTC TGAATGTCTT CAATAATTAC
    TTCAGCCTTG GATTTGATGC TCACGTCACA TTGGAGTTCC ATGAATCCCG AGAAGCAAAT
    CCAGAGAAAT TCAATAGTCG TTTTCGAAAT AAAATGTTCT ATGCAGGGGC AGCCTTTTCT
    GATTTCCTAC AAAGAAGTTC TAGAGATCTA TCCAAACACG TCAAAGTTGT CTGTGATGGG
    ACAGACCTCA CCCCAAAGAT CCAGGACCTG AAGTTCCAGT GTATAGTATT TTTAAATATT
    CCCAGATATT GTGCTGGCAC AATGCCTTGG GGAAACCCTG GAGATCATCA TGACTTTGAA
    CCCCAACGTC ATGATGACGG CTACATTGAA GTCATTGGAT TCACTATGGC CTCTTTGGCT
    GCCCTGCAAG TTGGGGGGCA CGGAGAAAGG CTGCACCAGT GTCGGGAAGT GATGCTTCTG
    ACTTACAAGT CCATTCCCAT GCAAGTGGAC GGGGAGCCAT GTCGGTTGGC ACCTGCTATG
    ATTCGGATCT CCTTGAGGAA TCAGGCCAAC ATGGTACAGA AGAGCAAGCG GAGAACCTCG
    ATGCCTTTGC TCAATGACAT CCATCAGGTG CAAGCTGCGG ACCTGCGGCG AGTGTCTGCT
    CCCCCCGGCT CCTTCACCAT TCCCCAGTCT GTCCCAGATC GCCTGAGGAT CCGTGTGAAC
    AAAATCAGTT TACAAGACTA TGAAGGACTC CACTATGACA AGGACAAACT CCGGGAAGCT
    TCCATCCCTC TGGGTATTCT AGTTGTTCGT GGAGACTGTG ATTTGGAGAC TTGCCGTATG
    TACATAGACC GTCTACAGGA GGACTTGCAG TCAGTTTCTT CTGGGTCACA GAGAGTTCAT
    TACCAGGACC AAGAAACCTC CTTCCCCAGG GCACTCTCAG CTCAGAGGCT GTCCCCACGG
    TGGTGCTTCC TAGATGCAAC GTCTGCTGAC CGCTTTTATC GAATAGACAG ATCTCAGGAA
    CATTTGCACT TTGTGATGGA GATTTCTCAC GATGAAATTT TTATTCTGGA CCCAGATATG
    GTGGTGTCAC AGCAGGCAGG GACCCCTCCA GGAATGCCTG ACCTGGTGGT TGAACAGGCT
    TCTGGGCTGT CTGACTGGTG GAACCCTGCC CTGAGGAAAC GTATGCTGAG TGACAGTGGG
    ATGATCACAC CCCACTATGA GGACTCAGAT CTGAAAGACT TCAGCCACTC CAGAGTGCTA
    CAGTCCCCAG TCTCTTCAGA AGATCATGCA ATTTTGCAGG CAGTCCTCAC CGGTGACCTT
    ATGAAGCTCA TGGAGAGCTA TAAGAATGGA GGGAGCTTAC TAATTCAGGG ACCAGGGCAC
    TGTTCTCTCC TTCACTACGC AGCTAAGACC GGCAATGGGG ACATTGTGAA GTACATCCTT
    GACCACGGAC CTGCAGAATT ACTGGATATG GCAGACAGTG AAACGGGCGA GACTGCACTG
    CACAAGGCTG CCTGCCAAAG GAACCGGGCT GTATGCCAGC TTCTGGTGGA TGCAGGAGCA
    TCTCTGAGAC AGACAGACTC CAAGGGCAAG ACACCCCAGG AACGAGCACA GCAGGCCGGG
    GACCCAGATT TGGCTGCTTA CCTAGAAAGC CGACAGAACT ATAAGATCAT TGGCCATGAA
    GACCTGGAAA CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74609
    Clone ID Related Accession (Same CDS sequence) XM_011241385.2 , XM_011241385.3
    Accession Version XM_011241385.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241385.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGGAAACCA AAGGCCGTCC TTTTGTGATC
    AAGCCCATCT CCTCCCCTCT CATGAAGCCT TTGCTTGTCT TTGTGAACCC AAAGAGTGGG
    GGAAACCAGG GAACCAAAGT CCTACAGATG TTTATGTGGT ACCTGAATCC ACGGCAAGTC
    TTTGATCTTT CTCAGGAAGG GCCAAAAGAT GCGCTGGAGA TGTACAGGAA GGTACCAAAT
    CTGAGAATTC TGGCCTGCGG TGGGGATGGA ACGGTGGGCT GGATCCTCTC CATCCTGGAC
    GAGCTGCAGC TGAGCCCACA GCCTCCTGTG GGGGTGCTTC CACTGGGGAC TGGGAATGAC
    CTGGCTCGGA CGCTCAACTG GGGAGGGGGT TACACCGATG AGCCTGTTTC TAAGATCCTG
    TGCCAGGTGG AAGATGGGAC CATTGTACAG TTGGATCGCT GGAACCTCCA CGTAGAGAGA
    AACCCAGACC TGCCGCCCGA AGAACTTGAA GATGGCGTGT GTAAGCTTCC TCTGAATGTC
    TTCAATAATT ACTTCAGCCT TGGATTTGAT GCTCACGTCA CATTGGAGTT CCATGAATCC
    CGAGAAGCAA ATCCAGAGAA ATTCAATAGT CGTTTTCGAA ATAAAATGTT CTATGCAGGG
    GCAGCCTTTT CTGATTTCCT ACAAAGAAGT TCTAGAGATC TATCCAAACA CGTCAAAGTT
    GTCTGTGATG GGACAGACCT CACCCCAAAG ATCCAGGACC TGAAGTTCCA GTGTATAGTA
    TTTTTAAATA TTCCCAGATA TTGTGCTGGC ACAATGCCTT GGGGAAACCC TGGAGATCAT
    CATGACTTTG AACCCCAACG TCATGATGAC GGCTACATTG AAGTCATTGG ATTCACTATG
    GCCTCTTTGG CTGCCCTGCA AGTTGGGGGG CACGGAGAAA GGCTGCACCA GTGTCGGGAA
    GTGATGCTTC TGACTTACAA GTCCATTCCC ATGCAAGTGG ACGGGGAGCC ATGTCGGTTG
    GCACCTGCTA TGATTCGGAT CTCCTTGAGG AATCAGGCCA ACATGGTACA GAAGAGCAAG
    CGGAGAACCT CGATGCCTTT GCTCAATGAT CCCCAGTCTG TCCCAGATCG CCTGAGGATC
    CGTGTGAACA AAATCAGTTT ACAAGACTAT GAAGGACTCC ACTATGACAA GGACAAACTC
    CGGGAAGCTT CCATCCCTCT GGGTATTCTA GTTGTTCGTG GAGACTGTGA TTTGGAGACT
    TGCCGTATGT ACATAGACCG TCTACAGGAG GACCAAGAAA CCTCCTTCCC CAGGGCACTC
    TCAGCTCAGA GGCTGTCCCC ACGGTGGTGC TTCCTAGATG CAACGTCTGC TGACCGCTTT
    TATCGAATAG ACAGATCTCA GGAACATTTG CACTTTGTGA TGGAGATTTC TCACGATGAA
    ATTTTTATTC TGGACCCAGA TATGGTGGTG TCACAGCAGG CAGGGACCCC TCCAGGAATG
    CCTGACCTGG TGGTTGAACA GGCTTCTGGG CTGTCTGACT GGTGGAACCC TGCCCTGAGG
    AAACGTATGC TGAGTGACAG TGGGATGATC ACACCCCACT ATGAGGACTC AGATCTGAAA
    GACTTCAGCC ACTCCAGAGT GCTACAGTCC CCAGTCTCTT CAGAAGATCA TGCAATTTTG
    CAGGCAGTCC TCACCGGTGA CCTTATGAAG CTCATGGAGA GCTATAAGAA TGGAGGGAGC
    TTACTAATTC AGGGACCAGG GCACTGTTCT CTCCTTCACT ACGCAGCTAA GACCGGCAAT
    GGGGACATTG TGAAGTACAT CCTTGACCAC GGACCTGCAG AATTACTGGA TATGGCAGAC
    AGTGAAACGG GCGAGACTGC ACTGCACAAG GCTGCCTGCC AAAGGAACCG GGCTGTATGC
    CAGCTTCTGG TGGATGCAGG AGCATCTCTG AGACAGACAG ACTCCAAGGG CAAGACACCC
    CAGGAACGAG CACAGCAGGC CGGGGACCCA GATTTGGCTG CTTACCTAGA AAGCCGACAG
    AACTATAAGA TCATTGGCCA TGAAGACCTG GAAACTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011239687.1
    CDS1008..4112
    Translation

    Target ORF information:

    RefSeq Version XM_011241385.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011241385.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGGAAACCA AAGGCCGTCC TTTTGTGATC
    AAGCCCATCT CCTCCCCTCT CATGAAGCCT TTGCTTGTCT TTGTGAACCC AAAGAGTGGG
    GGAAACCAGG GAACCAAAGT CCTACAGATG TTTATGTGGT ACCTGAATCC ACGGCAAGTC
    TTTGATCTTT CTCAGGAAGG GCCAAAAGAT GCGCTGGAGA TGTACAGGAA GGTACCAAAT
    CTGAGAATTC TGGCCTGCGG TGGGGATGGA ACGGTGGGCT GGATCCTCTC CATCCTGGAC
    GAGCTGCAGC TGAGCCCACA GCCTCCTGTG GGGGTGCTTC CACTGGGGAC TGGGAATGAC
    CTGGCTCGGA CGCTCAACTG GGGAGGGGGT TACACCGATG AGCCTGTTTC TAAGATCCTG
    TGCCAGGTGG AAGATGGGAC CATTGTACAG TTGGATCGCT GGAACCTCCA CGTAGAGAGA
    AACCCAGACC TGCCGCCCGA AGAACTTGAA GATGGCGTGT GTAAGCTTCC TCTGAATGTC
    TTCAATAATT ACTTCAGCCT TGGATTTGAT GCTCACGTCA CATTGGAGTT CCATGAATCC
    CGAGAAGCAA ATCCAGAGAA ATTCAATAGT CGTTTTCGAA ATAAAATGTT CTATGCAGGG
    GCAGCCTTTT CTGATTTCCT ACAAAGAAGT TCTAGAGATC TATCCAAACA CGTCAAAGTT
    GTCTGTGATG GGACAGACCT CACCCCAAAG ATCCAGGACC TGAAGTTCCA GTGTATAGTA
    TTTTTAAATA TTCCCAGATA TTGTGCTGGC ACAATGCCTT GGGGAAACCC TGGAGATCAT
    CATGACTTTG AACCCCAACG TCATGATGAC GGCTACATTG AAGTCATTGG ATTCACTATG
    GCCTCTTTGG CTGCCCTGCA AGTTGGGGGG CACGGAGAAA GGCTGCACCA GTGTCGGGAA
    GTGATGCTTC TGACTTACAA GTCCATTCCC ATGCAAGTGG ACGGGGAGCC ATGTCGGTTG
    GCACCTGCTA TGATTCGGAT CTCCTTGAGG AATCAGGCCA ACATGGTACA GAAGAGCAAG
    CGGAGAACCT CGATGCCTTT GCTCAATGAT CCCCAGTCTG TCCCAGATCG CCTGAGGATC
    CGTGTGAACA AAATCAGTTT ACAAGACTAT GAAGGACTCC ACTATGACAA GGACAAACTC
    CGGGAAGCTT CCATCCCTCT GGGTATTCTA GTTGTTCGTG GAGACTGTGA TTTGGAGACT
    TGCCGTATGT ACATAGACCG TCTACAGGAG GACCAAGAAA CCTCCTTCCC CAGGGCACTC
    TCAGCTCAGA GGCTGTCCCC ACGGTGGTGC TTCCTAGATG CAACGTCTGC TGACCGCTTT
    TATCGAATAG ACAGATCTCA GGAACATTTG CACTTTGTGA TGGAGATTTC TCACGATGAA
    ATTTTTATTC TGGACCCAGA TATGGTGGTG TCACAGCAGG CAGGGACCCC TCCAGGAATG
    CCTGACCTGG TGGTTGAACA GGCTTCTGGG CTGTCTGACT GGTGGAACCC TGCCCTGAGG
    AAACGTATGC TGAGTGACAG TGGGATGATC ACACCCCACT ATGAGGACTC AGATCTGAAA
    GACTTCAGCC ACTCCAGAGT GCTACAGTCC CCAGTCTCTT CAGAAGATCA TGCAATTTTG
    CAGGCAGTCC TCACCGGTGA CCTTATGAAG CTCATGGAGA GCTATAAGAA TGGAGGGAGC
    TTACTAATTC AGGGACCAGG GCACTGTTCT CTCCTTCACT ACGCAGCTAA GACCGGCAAT
    GGGGACATTG TGAAGTACAT CCTTGACCAC GGACCTGCAG AATTACTGGA TATGGCAGAC
    AGTGAAACGG GCGAGACTGC ACTGCACAAG GCTGCCTGCC AAAGGAACCG GGCTGTATGC
    CAGCTTCTGG TGGATGCAGG AGCATCTCTG AGACAGACAG ACTCCAAGGG CAAGACACCC
    CAGGAACGAG CACAGCAGGC CGGGGACCCA GATTTGGCTG CTTACCTAGA AAGCCGACAG
    AACTATAAGA TCATTGGCCA TGAAGACCTG GAAACTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81748
    Clone ID Related Accession (Same CDS sequence) XM_017321624.1 , XM_017321624.2
    Accession Version XM_017321624.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321624.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGACATCC ATCAGGTGCA AGCTGCGGAC
    CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC TTCACCATTC CCCAGTCTGT CCCAGATCGC
    CTGAGGATCC GTGTGAACAA AATCAGTTTA CAAGACTATG AAGGACTCCA CTATGACAAG
    GACAAACTCC GGGAAGCTTC CATCCCTCTG GGTATTCTAG TTGTTCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGACCGT CTACAGGAGG ACTTGCAGTC AGTTTCTTCT
    GGGTCACAGA GAGTTCATTA CCAGGACCAA GAAACCTCCT TCCCCAGGGC ACTCTCAGCT
    CAGAGGCTGT CCCCACGGTG GTGCTTCCTA GATGCAACGT CTGCTGACCG CTTTTATCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCTCACGA TGAAATTTTT
    ATTCTGGACC CAGATATGGT GGTGTCACAG CAGGCAGGGA CCCCTCCAGG AATGCCTGAC
    CTGGTGGTTG AACAGGCTTC TGGGCTGTCT GACTGGTGGA ACCCTGCCCT GAGGAAACGT
    ATGCTGAGTG ACAGTGGGAT GATCACACCC CACTATGAGG ACTCAGATCT GAAAGACTTC
    AGCCACTCCA GAGTGCTACA GTCCCCAGTC TCTTCAGAAG ATCATGCAAT TTTGCAGGCA
    GTCCTCACCG GTGACCTTAT GAAGCTCATG GAGAGCTATA AGAATGGAGG GAGCTTACTA
    ATTCAGGGAC CAGGGCACTG TTCTCTCCTT CACTACGCAG CTAAGACCGG CAATGGGGAC
    ATTGTGAAGT ACATCCTTGA CCACGGACCT GCAGAATTAC TGGATATGGC AGACAGTGAA
    ACGGGCGAGA CTGCACTGCA CAAGGCTGCC TGCCAAAGGA ACCGGGCTGT ATGCCAGCTT
    CTGGTGGATG CAGGAGCATC TCTGAGACAG ACAGACTCCA AGGGCAAGAC ACCCCAGGAA
    CGAGCACAGC AGGCCGGGGA CCCAGATTTG GCTGCTTACC TAGAAAGCCG ACAGAACTAT
    AAGATCATTG GCCATGAAGA CCTGGAAACT GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177113.1
    CDS1008..4346
    Translation

    Target ORF information:

    RefSeq Version XM_017321624.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321624.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGACATCC ATCAGGTGCA AGCTGCGGAC
    CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC TTCACCATTC CCCAGTCTGT CCCAGATCGC
    CTGAGGATCC GTGTGAACAA AATCAGTTTA CAAGACTATG AAGGACTCCA CTATGACAAG
    GACAAACTCC GGGAAGCTTC CATCCCTCTG GGTATTCTAG TTGTTCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGACCGT CTACAGGAGG ACTTGCAGTC AGTTTCTTCT
    GGGTCACAGA GAGTTCATTA CCAGGACCAA GAAACCTCCT TCCCCAGGGC ACTCTCAGCT
    CAGAGGCTGT CCCCACGGTG GTGCTTCCTA GATGCAACGT CTGCTGACCG CTTTTATCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCTCACGA TGAAATTTTT
    ATTCTGGACC CAGATATGGT GGTGTCACAG CAGGCAGGGA CCCCTCCAGG AATGCCTGAC
    CTGGTGGTTG AACAGGCTTC TGGGCTGTCT GACTGGTGGA ACCCTGCCCT GAGGAAACGT
    ATGCTGAGTG ACAGTGGGAT GATCACACCC CACTATGAGG ACTCAGATCT GAAAGACTTC
    AGCCACTCCA GAGTGCTACA GTCCCCAGTC TCTTCAGAAG ATCATGCAAT TTTGCAGGCA
    GTCCTCACCG GTGACCTTAT GAAGCTCATG GAGAGCTATA AGAATGGAGG GAGCTTACTA
    ATTCAGGGAC CAGGGCACTG TTCTCTCCTT CACTACGCAG CTAAGACCGG CAATGGGGAC
    ATTGTGAAGT ACATCCTTGA CCACGGACCT GCAGAATTAC TGGATATGGC AGACAGTGAA
    ACGGGCGAGA CTGCACTGCA CAAGGCTGCC TGCCAAAGGA ACCGGGCTGT ATGCCAGCTT
    CTGGTGGATG CAGGAGCATC TCTGAGACAG ACAGACTCCA AGGGCAAGAC ACCCCAGGAA
    CGAGCACAGC AGGCCGGGGA CCCAGATTTG GCTGCTTACC TAGAAAGCCG ACAGAACTAT
    AAGATCATTG GCCATGAAGA CCTGGAAACT GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81749
    Clone ID Related Accession (Same CDS sequence) XM_017321625.2 , XM_017321625.1
    Accession Version XM_017321625.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GACATCCATC AGGTGCAAGC TGCGGACCTG
    CGGCGAGTGT CTGCTCCCCC CGGCTCCTTC ACCATTCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACT TGCAGTCAGT TTCTTCTGGG
    TCACAGAGAG TTCATTACCA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTC CCCAGTCTCT TCAGAAGATC ATGCAATTTT GCAGGCAGTC
    CTCACCGGTG ACCTTATGAA GCTCATGGAG AGCTATAAGA ATGGAGGGAG CTTACTAATT
    CAGGGACCAG GGCACTGTTC TCTCCTTCAC TACGCAGCTA AGACCGGCAA TGGGGACATT
    GTGAAGTACA TCCTTGACCA CGGACCTGCA GAATTACTGG ATATGGCAGA CAGTGAAACG
    GGCGAGACTG CACTGCACAA GGCTGCCTGC CAAAGGAACC GGGCTGTATG CCAGCTTCTG
    GTGGATGCAG GAGCATCTCT GAGACAGACA GACTCCAAGG GCAAGACACC CCAGGAACGA
    GCACAGCAGG CCGGGGACCC AGATTTGGCT GCTTACCTAG AAAGCCGACA GAACTATAAG
    ATCATTGGCC ATGAAGACCT GGAAACTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177114.1
    CDS1008..4343
    Translation

    Target ORF information:

    RefSeq Version XM_017321625.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321625.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GACATCCATC AGGTGCAAGC TGCGGACCTG
    CGGCGAGTGT CTGCTCCCCC CGGCTCCTTC ACCATTCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACT TGCAGTCAGT TTCTTCTGGG
    TCACAGAGAG TTCATTACCA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTC CCCAGTCTCT TCAGAAGATC ATGCAATTTT GCAGGCAGTC
    CTCACCGGTG ACCTTATGAA GCTCATGGAG AGCTATAAGA ATGGAGGGAG CTTACTAATT
    CAGGGACCAG GGCACTGTTC TCTCCTTCAC TACGCAGCTA AGACCGGCAA TGGGGACATT
    GTGAAGTACA TCCTTGACCA CGGACCTGCA GAATTACTGG ATATGGCAGA CAGTGAAACG
    GGCGAGACTG CACTGCACAA GGCTGCCTGC CAAAGGAACC GGGCTGTATG CCAGCTTCTG
    GTGGATGCAG GAGCATCTCT GAGACAGACA GACTCCAAGG GCAAGACACC CCAGGAACGA
    GCACAGCAGG CCGGGGACCC AGATTTGGCT GCTTACCTAG AAAGCCGACA GAACTATAAG
    ATCATTGGCC ATGAAGACCT GGAAACTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81750
    Clone ID Related Accession (Same CDS sequence) XM_017321626.2 , XM_017321626.1
    Accession Version XM_017321626.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGACATCC ATCAGGTGCA AGCTGCGGAC
    CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC TTCACCATTC CCCAGTCTGT CCCAGATCGC
    CTGAGGATCC GTGTGAACAA AATCAGTTTA CAAGACTATG AAGGACTCCA CTATGACAAG
    GACAAACTCC GGGAAGCTTC CATCCCTCTG GGTATTCTAG TTGTTCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGACCGT CTACAGGAGG ACCAAGAAAC CTCCTTCCCC
    AGGGCACTCT CAGCTCAGAG GCTGTCCCCA CGGTGGTGCT TCCTAGATGC AACGTCTGCT
    GACCGCTTTT ATCGAATAGA CAGATCTCAG GAACATTTGC ACTTTGTGAT GGAGATTTCT
    CACGATGAAA TTTTTATTCT GGACCCAGAT ATGGTGGTGT CACAGCAGGC AGGGACCCCT
    CCAGGAATGC CTGACCTGGT GGTTGAACAG GCTTCTGGGC TGTCTGACTG GTGGAACCCT
    GCCCTGAGGA AACGTATGCT GAGTGACAGT GGGATGATCA CACCCCACTA TGAGGACTCA
    GATCTGAAAG ACTTCAGCCA CTCCAGAGTG CTACAGTCCC CAGTCTCTTC AGAAGATCAT
    GCAATTTTGC AGGCAGTCCT CACCGGTGAC CTTATGAAGC TCATGGAGAG CTATAAGAAT
    GGAGGGAGCT TACTAATTCA GGGACCAGGG CACTGTTCTC TCCTTCACTA CGCAGCTAAG
    ACCGGCAATG GGGACATTGT GAAGTACATC CTTGACCACG GACCTGCAGA ATTACTGGAT
    ATGGCAGACA GTGAAACGGG CGAGACTGCA CTGCACAAGG CTGCCTGCCA AAGGAACCGG
    GCTGTATGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GACAGACAGA CTCCAAGGGC
    AAGACACCCC AGGAACGAGC ACAGCAGGCC GGGGACCCAG ATTTGGCTGC TTACCTAGAA
    AGCCGACAGA ACTATAAGAT CATTGGCCAT GAAGACCTGG AAACTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177115.1
    CDS1008..4301
    Translation

    Target ORF information:

    RefSeq Version XM_017321626.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321626.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGACATCC ATCAGGTGCA AGCTGCGGAC
    CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC TTCACCATTC CCCAGTCTGT CCCAGATCGC
    CTGAGGATCC GTGTGAACAA AATCAGTTTA CAAGACTATG AAGGACTCCA CTATGACAAG
    GACAAACTCC GGGAAGCTTC CATCCCTCTG GGTATTCTAG TTGTTCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGACCGT CTACAGGAGG ACCAAGAAAC CTCCTTCCCC
    AGGGCACTCT CAGCTCAGAG GCTGTCCCCA CGGTGGTGCT TCCTAGATGC AACGTCTGCT
    GACCGCTTTT ATCGAATAGA CAGATCTCAG GAACATTTGC ACTTTGTGAT GGAGATTTCT
    CACGATGAAA TTTTTATTCT GGACCCAGAT ATGGTGGTGT CACAGCAGGC AGGGACCCCT
    CCAGGAATGC CTGACCTGGT GGTTGAACAG GCTTCTGGGC TGTCTGACTG GTGGAACCCT
    GCCCTGAGGA AACGTATGCT GAGTGACAGT GGGATGATCA CACCCCACTA TGAGGACTCA
    GATCTGAAAG ACTTCAGCCA CTCCAGAGTG CTACAGTCCC CAGTCTCTTC AGAAGATCAT
    GCAATTTTGC AGGCAGTCCT CACCGGTGAC CTTATGAAGC TCATGGAGAG CTATAAGAAT
    GGAGGGAGCT TACTAATTCA GGGACCAGGG CACTGTTCTC TCCTTCACTA CGCAGCTAAG
    ACCGGCAATG GGGACATTGT GAAGTACATC CTTGACCACG GACCTGCAGA ATTACTGGAT
    ATGGCAGACA GTGAAACGGG CGAGACTGCA CTGCACAAGG CTGCCTGCCA AAGGAACCGG
    GCTGTATGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GACAGACAGA CTCCAAGGGC
    AAGACACCCC AGGAACGAGC ACAGCAGGCC GGGGACCCAG ATTTGGCTGC TTACCTAGAA
    AGCCGACAGA ACTATAAGAT CATTGGCCAT GAAGACCTGG AAACTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81751
    Clone ID Related Accession (Same CDS sequence) XM_017321627.2 , XM_017321627.1
    Accession Version XM_017321627.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGATCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACT TGCAGTCAGT TTCTTCTGGG
    TCACAGAGAG TTCATTACCA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTC CCCAGTCTCT TCAGAAGATC ATGCAATTTT GCAGGCAGTC
    CTCACCGGTG ACCTTATGAA GCTCATGGAG AGCTATAAGA ATGGAGGGAG CTTACTAATT
    CAGGGACCAG GGCACTGTTC TCTCCTTCAC TACGCAGCTA AGACCGGCAA TGGGGACATT
    GTGAAGTACA TCCTTGACCA CGGACCTGCA GAATTACTGG ATATGGCAGA CAGTGAAACG
    GGCGAGACTG CACTGCACAA GGCTGCCTGC CAAAGGAACC GGGCTGTATG CCAGCTTCTG
    GTGGATGCAG GAGCATCTCT GAGACAGACA GACTCCAAGG GCAAGACACC CCAGGAACGA
    GCACAGCAGG CCGGGGACCC AGATTTGGCT GCTTACCTAG AAAGCCGACA GAACTATAAG
    ATCATTGGCC ATGAAGACCT GGAAACTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177116.1
    CDS1008..4283
    Translation

    Target ORF information:

    RefSeq Version XM_017321627.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321627.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGATCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACT TGCAGTCAGT TTCTTCTGGG
    TCACAGAGAG TTCATTACCA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTC CCCAGTCTCT TCAGAAGATC ATGCAATTTT GCAGGCAGTC
    CTCACCGGTG ACCTTATGAA GCTCATGGAG AGCTATAAGA ATGGAGGGAG CTTACTAATT
    CAGGGACCAG GGCACTGTTC TCTCCTTCAC TACGCAGCTA AGACCGGCAA TGGGGACATT
    GTGAAGTACA TCCTTGACCA CGGACCTGCA GAATTACTGG ATATGGCAGA CAGTGAAACG
    GGCGAGACTG CACTGCACAA GGCTGCCTGC CAAAGGAACC GGGCTGTATG CCAGCTTCTG
    GTGGATGCAG GAGCATCTCT GAGACAGACA GACTCCAAGG GCAAGACACC CCAGGAACGA
    GCACAGCAGG CCGGGGACCC AGATTTGGCT GCTTACCTAG AAAGCCGACA GAACTATAAG
    ATCATTGGCC ATGAAGACCT GGAAACTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81752
    Clone ID Related Accession (Same CDS sequence) XM_017321628.1 , XM_017321628.2
    Accession Version XM_017321628.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321628.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GATCCCCAGT CTGTCCCAGA TCGCCTGAGG
    ATCCGTGTGA ACAAAATCAG TTTACAAGAC TATGAAGGAC TCCACTATGA CAAGGACAAA
    CTCCGGGAAG CTTCCATCCC TCTGGGTATT CTAGTTGTTC GTGGAGACTG TGATTTGGAG
    ACTTGCCGTA TGTACATAGA CCGTCTACAG GAGGACTTGC AGTCAGTTTC TTCTGGGTCA
    CAGAGAGTTC ATTACCAGGA CCAAGAAACC TCCTTCCCCA GGGCACTCTC AGCTCAGAGG
    CTGTCCCCAC GGTGGTGCTT CCTAGATGCA ACGTCTGCTG ACCGCTTTTA TCGAATAGAC
    AGATCTCAGG AACATTTGCA CTTTGTGATG GAGATTTCTC ACGATGAAAT TTTTATTCTG
    GACCCAGATA TGGTGGTGTC ACAGCAGGCA GGGACCCCTC CAGGAATGCC TGACCTGGTG
    GTTGAACAGG CTTCTGGGCT GTCTGACTGG TGGAACCCTG CCCTGAGGAA ACGTATGCTG
    AGTGACAGTG GGATGATCAC ACCCCACTAT GAGGACTCAG ATCTGAAAGA CTTCAGCCAC
    TCCAGAGTGC TACAGTCCCC AGTCTCTTCA GAAGATCATG CAATTTTGCA GGCAGTCCTC
    ACCGGTGACC TTATGAAGCT CATGGAGAGC TATAAGAATG GAGGGAGCTT ACTAATTCAG
    GGACCAGGGC ACTGTTCTCT CCTTCACTAC GCAGCTAAGA CCGGCAATGG GGACATTGTG
    AAGTACATCC TTGACCACGG ACCTGCAGAA TTACTGGATA TGGCAGACAG TGAAACGGGC
    GAGACTGCAC TGCACAAGGC TGCCTGCCAA AGGAACCGGG CTGTATGCCA GCTTCTGGTG
    GATGCAGGAG CATCTCTGAG ACAGACAGAC TCCAAGGGCA AGACACCCCA GGAACGAGCA
    CAGCAGGCCG GGGACCCAGA TTTGGCTGCT TACCTAGAAA GCCGACAGAA CTATAAGATC
    ATTGGCCATG AAGACCTGGA AACTGCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177117.1
    CDS1008..4280
    Translation

    Target ORF information:

    RefSeq Version XM_017321628.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321628.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GATCCCCAGT CTGTCCCAGA TCGCCTGAGG
    ATCCGTGTGA ACAAAATCAG TTTACAAGAC TATGAAGGAC TCCACTATGA CAAGGACAAA
    CTCCGGGAAG CTTCCATCCC TCTGGGTATT CTAGTTGTTC GTGGAGACTG TGATTTGGAG
    ACTTGCCGTA TGTACATAGA CCGTCTACAG GAGGACTTGC AGTCAGTTTC TTCTGGGTCA
    CAGAGAGTTC ATTACCAGGA CCAAGAAACC TCCTTCCCCA GGGCACTCTC AGCTCAGAGG
    CTGTCCCCAC GGTGGTGCTT CCTAGATGCA ACGTCTGCTG ACCGCTTTTA TCGAATAGAC
    AGATCTCAGG AACATTTGCA CTTTGTGATG GAGATTTCTC ACGATGAAAT TTTTATTCTG
    GACCCAGATA TGGTGGTGTC ACAGCAGGCA GGGACCCCTC CAGGAATGCC TGACCTGGTG
    GTTGAACAGG CTTCTGGGCT GTCTGACTGG TGGAACCCTG CCCTGAGGAA ACGTATGCTG
    AGTGACAGTG GGATGATCAC ACCCCACTAT GAGGACTCAG ATCTGAAAGA CTTCAGCCAC
    TCCAGAGTGC TACAGTCCCC AGTCTCTTCA GAAGATCATG CAATTTTGCA GGCAGTCCTC
    ACCGGTGACC TTATGAAGCT CATGGAGAGC TATAAGAATG GAGGGAGCTT ACTAATTCAG
    GGACCAGGGC ACTGTTCTCT CCTTCACTAC GCAGCTAAGA CCGGCAATGG GGACATTGTG
    AAGTACATCC TTGACCACGG ACCTGCAGAA TTACTGGATA TGGCAGACAG TGAAACGGGC
    GAGACTGCAC TGCACAAGGC TGCCTGCCAA AGGAACCGGG CTGTATGCCA GCTTCTGGTG
    GATGCAGGAG CATCTCTGAG ACAGACAGAC TCCAAGGGCA AGACACCCCA GGAACGAGCA
    CAGCAGGCCG GGGACCCAGA TTTGGCTGCT TACCTAGAAA GCCGACAGAA CTATAAGATC
    ATTGGCCATG AAGACCTGGA AACTGCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81753
    Clone ID Related Accession (Same CDS sequence) XM_017321629.2 , XM_017321629.1
    Accession Version XM_017321629.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGATCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACC AAGAAACCTC CTTCCCCAGG
    GCACTCTCAG CTCAGAGGCT GTCCCCACGG TGGTGCTTCC TAGATGCAAC GTCTGCTGAC
    CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCTCAC
    GATGAAATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCAGGCAGG GACCCCTCCA
    GGAATGCCTG ACCTGGTGGT TGAACAGGCT TCTGGGCTGT CTGACTGGTG GAACCCTGCC
    CTGAGGAAAC GTATGCTGAG TGACAGTGGG ATGATCACAC CCCACTATGA GGACTCAGAT
    CTGAAAGACT TCAGCCACTC CAGAGTGCTA CAGTCCCCAG TCTCTTCAGA AGATCATGCA
    ATTTTGCAGG CAGTCCTCAC CGGTGACCTT ATGAAGCTCA TGGAGAGCTA TAAGAATGGA
    GGGAGCTTAC TAATTCAGGG ACCAGGGCAC TGTTCTCTCC TTCACTACGC AGCTAAGACC
    GGCAATGGGG ACATTGTGAA GTACATCCTT GACCACGGAC CTGCAGAATT ACTGGATATG
    GCAGACAGTG AAACGGGCGA GACTGCACTG CACAAGGCTG CCTGCCAAAG GAACCGGGCT
    GTATGCCAGC TTCTGGTGGA TGCAGGAGCA TCTCTGAGAC AGACAGACTC CAAGGGCAAG
    ACACCCCAGG AACGAGCACA GCAGGCCGGG GACCCAGATT TGGCTGCTTA CCTAGAAAGC
    CGACAGAACT ATAAGATCAT TGGCCATGAA GACCTGGAAA CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177118.1
    CDS1008..4238
    Translation

    Target ORF information:

    RefSeq Version XM_017321629.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321629.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGATCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACC AAGAAACCTC CTTCCCCAGG
    GCACTCTCAG CTCAGAGGCT GTCCCCACGG TGGTGCTTCC TAGATGCAAC GTCTGCTGAC
    CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCTCAC
    GATGAAATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCAGGCAGG GACCCCTCCA
    GGAATGCCTG ACCTGGTGGT TGAACAGGCT TCTGGGCTGT CTGACTGGTG GAACCCTGCC
    CTGAGGAAAC GTATGCTGAG TGACAGTGGG ATGATCACAC CCCACTATGA GGACTCAGAT
    CTGAAAGACT TCAGCCACTC CAGAGTGCTA CAGTCCCCAG TCTCTTCAGA AGATCATGCA
    ATTTTGCAGG CAGTCCTCAC CGGTGACCTT ATGAAGCTCA TGGAGAGCTA TAAGAATGGA
    GGGAGCTTAC TAATTCAGGG ACCAGGGCAC TGTTCTCTCC TTCACTACGC AGCTAAGACC
    GGCAATGGGG ACATTGTGAA GTACATCCTT GACCACGGAC CTGCAGAATT ACTGGATATG
    GCAGACAGTG AAACGGGCGA GACTGCACTG CACAAGGCTG CCTGCCAAAG GAACCGGGCT
    GTATGCCAGC TTCTGGTGGA TGCAGGAGCA TCTCTGAGAC AGACAGACTC CAAGGGCAAG
    ACACCCCAGG AACGAGCACA GCAGGCCGGG GACCCAGATT TGGCTGCTTA CCTAGAAAGC
    CGACAGAACT ATAAGATCAT TGGCCATGAA GACCTGGAAA CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81754
    Clone ID Related Accession (Same CDS sequence) XM_017321630.1 , XM_017321630.2
    Accession Version XM_017321630.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321630.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGACATCC ATCAGGTGCA AGCTGCGGAC
    CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC TTCACCATTC CCCAGTCTGT CCCAGATCGC
    CTGAGGATCC GTGTGAACAA AATCAGTTTA CAAGACTATG AAGGACTCCA CTATGACAAG
    GACAAACTCC GGGAAGCTTC CATCCCTCTG GGTATTCTAG TTGTTCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGACCGT CTACAGGAGG ACTTGCAGTC AGTTTCTTCT
    GGGTCACAGA GAGTTCATTA CCAGGACCAA GAAACCTCCT TCCCCAGGGC ACTCTCAGCT
    CAGAGGCTGT CCCCACGGTG GTGCTTCCTA GATGCAACGT CTGCTGACCG CTTTTATCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCTCACGA TGAAATTTTT
    ATTCTGGACC CAGATATGGT GGTGTCACAG CAGGCAGGGA CCCCTCCAGG AATGCCTGAC
    CTGGTGGTTG AACAGGCTTC TGGGCTGTCT GACTGGTGGA ACCCTGCCCT GAGGAAACGT
    ATGCTGAGTG ACAGTGGGAT GATCACACCC CACTATGAGG ACTCAGATCT GAAAGACTTC
    AGCCACTCCA GAGTGCTACA GTGTGATCAT GGTTGGAAAG CAACATGCTG CTTGGAATTT
    TGCAGAAGAA CTGATACTTT GGGCTATGCG AAATCATCTT ATCTGCCCAT ATTTTGCTAC
    AGGAACACGT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177119.1
    CDS1008..4022
    Translation

    Target ORF information:

    RefSeq Version XM_017321630.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321630.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGACATCC ATCAGGTGCA AGCTGCGGAC
    CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC TTCACCATTC CCCAGTCTGT CCCAGATCGC
    CTGAGGATCC GTGTGAACAA AATCAGTTTA CAAGACTATG AAGGACTCCA CTATGACAAG
    GACAAACTCC GGGAAGCTTC CATCCCTCTG GGTATTCTAG TTGTTCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGACCGT CTACAGGAGG ACTTGCAGTC AGTTTCTTCT
    GGGTCACAGA GAGTTCATTA CCAGGACCAA GAAACCTCCT TCCCCAGGGC ACTCTCAGCT
    CAGAGGCTGT CCCCACGGTG GTGCTTCCTA GATGCAACGT CTGCTGACCG CTTTTATCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCTCACGA TGAAATTTTT
    ATTCTGGACC CAGATATGGT GGTGTCACAG CAGGCAGGGA CCCCTCCAGG AATGCCTGAC
    CTGGTGGTTG AACAGGCTTC TGGGCTGTCT GACTGGTGGA ACCCTGCCCT GAGGAAACGT
    ATGCTGAGTG ACAGTGGGAT GATCACACCC CACTATGAGG ACTCAGATCT GAAAGACTTC
    AGCCACTCCA GAGTGCTACA GTGTGATCAT GGTTGGAAAG CAACATGCTG CTTGGAATTT
    TGCAGAAGAA CTGATACTTT GGGCTATGCG AAATCATCTT ATCTGCCCAT ATTTTGCTAC
    AGGAACACGT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu89226
    Clone ID Related Accession (Same CDS sequence) XM_030255453.1
    Accession Version XM_030255453.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGCAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GACATCCATC AGGTGCAAGC TGCGGACCTG
    CGGCGAGTGT CTGCTCCCCC CGGCTCCTTC ACCATTCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACC AAGAAACCTC CTTCCCCAGG
    GCACTCTCAG CTCAGAGGCT GTCCCCACGG TGGTGCTTCC TAGATGCAAC GTCTGCTGAC
    CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCTCAC
    GATGAAATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCAGGCAGG GACCCCTCCA
    GGAATGCCTG ACCTGGTGGT TGAACAGGCT TCTGGGCTGT CTGACTGGTG GAACCCTGCC
    CTGAGGAAAC GTATGCTGAG TGACAGTGGG ATGATCACAC CCCACTATGA GGACTCAGAT
    CTGAAAGACT TCAGCCACTC CAGAGTGCTA CAGTCCCCAG TCTCTTCAGA AGATCATGCA
    ATTTTGCAGG CAGTCCTCAC CGGTGACCTT ATGAAGCTCA TGGAGAGCTA TAAGAATGGA
    GGGAGCTTAC TAATTCAGGG ACCAGGGCAC TGTTCTCTCC TTCACTACGC AGCTAAGACC
    GGCAATGGGG ACATTGTGAA GTACATCCTT GACCACGGAC CTGCAGAATT ACTGGATATG
    GCAGACAGTG AAACGGGCGA GACTGCACTG CACAAGGCTG CCTGCCAAAG GAACCGGGCT
    GTATGCCAGC TTCTGGTGGA TGCAGGAGCA TCTCTGAGAC AGACAGACTC CAAGGGCAAG
    ACACCCCAGG AACGAGCACA GCAGGCCGGG GACCCAGATT TGGCTGCTTA CCTAGAAAGC
    CGACAGAACT ATAAGATCAT TGGCCATGAA GACCTGGAAA CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030111313.1
    CDS1008..4178
    Translation

    Target ORF information:

    RefSeq Version XM_030255453.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030255453.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGCAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GACATCCATC AGGTGCAAGC TGCGGACCTG
    CGGCGAGTGT CTGCTCCCCC CGGCTCCTTC ACCATTCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACC AAGAAACCTC CTTCCCCAGG
    GCACTCTCAG CTCAGAGGCT GTCCCCACGG TGGTGCTTCC TAGATGCAAC GTCTGCTGAC
    CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCTCAC
    GATGAAATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCAGGCAGG GACCCCTCCA
    GGAATGCCTG ACCTGGTGGT TGAACAGGCT TCTGGGCTGT CTGACTGGTG GAACCCTGCC
    CTGAGGAAAC GTATGCTGAG TGACAGTGGG ATGATCACAC CCCACTATGA GGACTCAGAT
    CTGAAAGACT TCAGCCACTC CAGAGTGCTA CAGTCCCCAG TCTCTTCAGA AGATCATGCA
    ATTTTGCAGG CAGTCCTCAC CGGTGACCTT ATGAAGCTCA TGGAGAGCTA TAAGAATGGA
    GGGAGCTTAC TAATTCAGGG ACCAGGGCAC TGTTCTCTCC TTCACTACGC AGCTAAGACC
    GGCAATGGGG ACATTGTGAA GTACATCCTT GACCACGGAC CTGCAGAATT ACTGGATATG
    GCAGACAGTG AAACGGGCGA GACTGCACTG CACAAGGCTG CCTGCCAAAG GAACCGGGCT
    GTATGCCAGC TTCTGGTGGA TGCAGGAGCA TCTCTGAGAC AGACAGACTC CAAGGGCAAG
    ACACCCCAGG AACGAGCACA GCAGGCCGGG GACCCAGATT TGGCTGCTTA CCTAGAAAGC
    CGACAGAACT ATAAGATCAT TGGCCATGAA GACCTGGAAA CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu01881
    Clone ID Related Accession (Same CDS sequence) NM_001081206.2 , NM_001081206.1
    Accession Version NM_001081206.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-26
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK081360.1, AC159480.5 and AC154019.3. On Mar 19, 2018 this sequence version replaced NM_001081206.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK081360.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGCAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GACATCCATC AGGTGCAAGC TGCGGACCTG
    CGGCGAGTGT CTGCTCCCCC CGGCTCCTTC ACCATTCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACT TGCAGTCAGT TTCTTCTGGG
    TCACAGAGAG TTCATTACCA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTC CCCAGTCTCT TCAGAAGATC ATGCAATTTT GCAGGCAGTC
    CTCACCGGTG ACCTTATGAA GCTCATGGAG AGCTATAAGA ATGGAGGGAG CTTACTAATT
    CAGGGACCAG GGCACTGTTC TCTCCTTCAC TACGCAGCTA AGACCGGCAA TGGGGACATT
    GTGAAGTACA TCCTTGACCA CGGACCTGCA GAATTACTGG ATATGGCAGA CAGTGAAACG
    GGCGAGACTG CACTGCACAA GGCTGCCTGC CAAAGGAACC GGGCTGTATG CCAGCTTCTG
    GTGGATGCAG GAGCATCTCT GAGACAGACA GACTCCAAGG GCAAGACACC CCAGGAACGA
    GCACAGCAGG CCGGGGACCC AGATTTGGCT GCTTACCTAG AAAGCCGACA GAACTATAAG
    ATCATTGGCC ATGAAGACCT GGAAACTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001074675.1
    CDS29..3244
    Translation

    Target ORF information:

    RefSeq Version NM_001081206.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081206.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGCAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GACATCCATC AGGTGCAAGC TGCGGACCTG
    CGGCGAGTGT CTGCTCCCCC CGGCTCCTTC ACCATTCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACT TGCAGTCAGT TTCTTCTGGG
    TCACAGAGAG TTCATTACCA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTC CCCAGTCTCT TCAGAAGATC ATGCAATTTT GCAGGCAGTC
    CTCACCGGTG ACCTTATGAA GCTCATGGAG AGCTATAAGA ATGGAGGGAG CTTACTAATT
    CAGGGACCAG GGCACTGTTC TCTCCTTCAC TACGCAGCTA AGACCGGCAA TGGGGACATT
    GTGAAGTACA TCCTTGACCA CGGACCTGCA GAATTACTGG ATATGGCAGA CAGTGAAACG
    GGCGAGACTG CACTGCACAA GGCTGCCTGC CAAAGGAACC GGGCTGTATG CCAGCTTCTG
    GTGGATGCAG GAGCATCTCT GAGACAGACA GACTCCAAGG GCAAGACACC CCAGGAACGA
    GCACAGCAGG CCGGGGACCC AGATTTGGCT GCTTACCTAG AAAGCCGACA GAACTATAAG
    ATCATTGGCC ATGAAGACCT GGAAACTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu01881
    Clone ID Related Accession (Same CDS sequence) NM_001081206.2 , NM_001081206.1
    Accession Version NM_001081206.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-28
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC153625.2, AC159480.5, AC114574.8 and AC154019.3. On Feb 6, 2007 this sequence version replaced XM_355752.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK081360.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGCAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GACATCCATC AGGTGCAAGC TGCGGACCTG
    CGGCGAGTGT CTGCTCCCCC CGGCTCCTTC ACCATTCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACT TGCAGTCAGT TTCTTCTGGG
    TCACAGAGAG TTCATTACCA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTC CCCAGTCTCT TCAGAAGATC ATGCAATTTT GCAGGCAGTC
    CTCACCGGTG ACCTTATGAA GCTCATGGAG AGCTATAAGA ATGGAGGGAG CTTACTAATT
    CAGGGACCAG GGCACTGTTC TCTCCTTCAC TACGCAGCTA AGACCGGCAA TGGGGACATT
    GTGAAGTACA TCCTTGACCA CGGACCTGCA GAATTACTGG ATATGGCAGA CAGTGAAACG
    GGCGAGACTG CACTGCACAA GGCTGCCTGC CAAAGGAACC GGGCTGTATG CCAGCTTCTG
    GTGGATGCAG GAGCATCTCT GAGACAGACA GACTCCAAGG GCAAGACACC CCAGGAACGA
    GCACAGCAGG CCGGGGACCC AGATTTGGCT GCTTACCTAG AAAGCCGACA GAACTATAAG
    ATCATTGGCC ATGAAGACCT GGAAACTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001074675.1
    CDS29..3244
    Translation

    Target ORF information:

    RefSeq Version NM_001081206.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081206.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGCAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GACATCCATC AGGTGCAAGC TGCGGACCTG
    CGGCGAGTGT CTGCTCCCCC CGGCTCCTTC ACCATTCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACT TGCAGTCAGT TTCTTCTGGG
    TCACAGAGAG TTCATTACCA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTC CCCAGTCTCT TCAGAAGATC ATGCAATTTT GCAGGCAGTC
    CTCACCGGTG ACCTTATGAA GCTCATGGAG AGCTATAAGA ATGGAGGGAG CTTACTAATT
    CAGGGACCAG GGCACTGTTC TCTCCTTCAC TACGCAGCTA AGACCGGCAA TGGGGACATT
    GTGAAGTACA TCCTTGACCA CGGACCTGCA GAATTACTGG ATATGGCAGA CAGTGAAACG
    GGCGAGACTG CACTGCACAA GGCTGCCTGC CAAAGGAACC GGGCTGTATG CCAGCTTCTG
    GTGGATGCAG GAGCATCTCT GAGACAGACA GACTCCAAGG GCAAGACACC CCAGGAACGA
    GCACAGCAGG CCGGGGACCC AGATTTGGCT GCTTACCTAG AAAGCCGACA GAACTATAAG
    ATCATTGGCC ATGAAGACCT GGAAACTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37363
    Clone ID Related Accession (Same CDS sequence) NM_001347104.2 , NM_001347104.1
    Accession Version NM_001347104.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-26
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC184160.6, AC159480.5, AC114574.8 and AC154019.3. On Mar 18, 2018 this sequence version replaced NM_001347104.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660825.50779.1, SRR7345562.1982390.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGCAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GATCCCCAGT CTGTCCCAGA TCGCCTGAGG
    ATCCGTGTGA ACAAAATCAG TTTACAAGAC TATGAAGGAC TCCACTATGA CAAGGACAAA
    CTCCGGGAAG CTTCCATCCC TCTGGGTATT CTAGTTGTTC GTGGAGACTG TGATTTGGAG
    ACTTGCCGTA TGTACATAGA CCGTCTACAG GAGGACTTGC AGTCAGTTTC TTCTGGGTCA
    CAGAGAGTTC ATTACCAGGA CCAAGAAACC TCCTTCCCCA GGGCACTCTC AGCTCAGAGG
    CTGTCCCCAC GGTGGTGCTT CCTAGATGCA ACGTCTGCTG ACCGCTTTTA TCGAATAGAC
    AGATCTCAGG AACATTTGCA CTTTGTGATG GAGATTTCTC ACGATGAAAT TTTTATTCTG
    GACCCAGATA TGGTGGTGTC ACAGCAGGCA GGGACCCCTC CAGGAATGCC TGACCTGGTG
    GTTGAACAGG CTTCTGGGCT GTCTGACTGG TGGAACCCTG CCCTGAGGAA ACGTATGCTG
    AGTGACAGTG GGATGATCAC ACCCCACTAT GAGGACTCAG ATCTGAAAGA CTTCAGCCAC
    TCCAGAGTGC TACAGTCCCC AGTCTCTTCA GAAGATCATG CAATTTTGCA GGCAGTCCTC
    ACCGGTGACC TTATGAAGCT CATGGAGAGC TATAAGAATG GAGGGAGCTT ACTAATTCAG
    GGACCAGGGC ACTGTTCTCT CCTTCACTAC GCAGCTAAGA CCGGCAATGG GGACATTGTG
    AAGTACATCC TTGACCACGG ACCTGCAGAA TTACTGGATA TGGCAGACAG TGAAACGGGC
    GAGACTGCAC TGCACAAGGC TGCCTGCCAA AGGAACCGGG CTGTATGCCA GCTTCTGGTG
    GATGCAGGAG CATCTCTGAG ACAGACAGAC TCCAAGGGCA AGACACCCCA GGAACGAGCA
    CAGCAGGCCG GGGACCCAGA TTTGGCTGCT TACCTAGAAA GCCGACAGAA CTATAAGATC
    ATTGGCCATG AAGACCTGGA AACTGCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334033.1
    CDS29..3181
    Translation

    Target ORF information:

    RefSeq Version NM_001347104.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347104.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGCAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GATCCCCAGT CTGTCCCAGA TCGCCTGAGG
    ATCCGTGTGA ACAAAATCAG TTTACAAGAC TATGAAGGAC TCCACTATGA CAAGGACAAA
    CTCCGGGAAG CTTCCATCCC TCTGGGTATT CTAGTTGTTC GTGGAGACTG TGATTTGGAG
    ACTTGCCGTA TGTACATAGA CCGTCTACAG GAGGACTTGC AGTCAGTTTC TTCTGGGTCA
    CAGAGAGTTC ATTACCAGGA CCAAGAAACC TCCTTCCCCA GGGCACTCTC AGCTCAGAGG
    CTGTCCCCAC GGTGGTGCTT CCTAGATGCA ACGTCTGCTG ACCGCTTTTA TCGAATAGAC
    AGATCTCAGG AACATTTGCA CTTTGTGATG GAGATTTCTC ACGATGAAAT TTTTATTCTG
    GACCCAGATA TGGTGGTGTC ACAGCAGGCA GGGACCCCTC CAGGAATGCC TGACCTGGTG
    GTTGAACAGG CTTCTGGGCT GTCTGACTGG TGGAACCCTG CCCTGAGGAA ACGTATGCTG
    AGTGACAGTG GGATGATCAC ACCCCACTAT GAGGACTCAG ATCTGAAAGA CTTCAGCCAC
    TCCAGAGTGC TACAGTCCCC AGTCTCTTCA GAAGATCATG CAATTTTGCA GGCAGTCCTC
    ACCGGTGACC TTATGAAGCT CATGGAGAGC TATAAGAATG GAGGGAGCTT ACTAATTCAG
    GGACCAGGGC ACTGTTCTCT CCTTCACTAC GCAGCTAAGA CCGGCAATGG GGACATTGTG
    AAGTACATCC TTGACCACGG ACCTGCAGAA TTACTGGATA TGGCAGACAG TGAAACGGGC
    GAGACTGCAC TGCACAAGGC TGCCTGCCAA AGGAACCGGG CTGTATGCCA GCTTCTGGTG
    GATGCAGGAG CATCTCTGAG ACAGACAGAC TCCAAGGGCA AGACACCCCA GGAACGAGCA
    CAGCAGGCCG GGGACCCAGA TTTGGCTGCT TACCTAGAAA GCCGACAGAA CTATAAGATC
    ATTGGCCATG AAGACCTGGA AACTGCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37363
    Clone ID Related Accession (Same CDS sequence) NM_001347104.2 , NM_001347104.1
    Accession Version NM_001347104.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-12-08
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC184160.6, AC159480.5, AC114574.8 and AC154019.3. On Nov 15, 2016 this sequence version replaced XM_006506260.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660823.50779.1, SRR1660825.50779.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGCAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GATCCCCAGT CTGTCCCAGA TCGCCTGAGG
    ATCCGTGTGA ACAAAATCAG TTTACAAGAC TATGAAGGAC TCCACTATGA CAAGGACAAA
    CTCCGGGAAG CTTCCATCCC TCTGGGTATT CTAGTTGTTC GTGGAGACTG TGATTTGGAG
    ACTTGCCGTA TGTACATAGA CCGTCTACAG GAGGACTTGC AGTCAGTTTC TTCTGGGTCA
    CAGAGAGTTC ATTACCAGGA CCAAGAAACC TCCTTCCCCA GGGCACTCTC AGCTCAGAGG
    CTGTCCCCAC GGTGGTGCTT CCTAGATGCA ACGTCTGCTG ACCGCTTTTA TCGAATAGAC
    AGATCTCAGG AACATTTGCA CTTTGTGATG GAGATTTCTC ACGATGAAAT TTTTATTCTG
    GACCCAGATA TGGTGGTGTC ACAGCAGGCA GGGACCCCTC CAGGAATGCC TGACCTGGTG
    GTTGAACAGG CTTCTGGGCT GTCTGACTGG TGGAACCCTG CCCTGAGGAA ACGTATGCTG
    AGTGACAGTG GGATGATCAC ACCCCACTAT GAGGACTCAG ATCTGAAAGA CTTCAGCCAC
    TCCAGAGTGC TACAGTCCCC AGTCTCTTCA GAAGATCATG CAATTTTGCA GGCAGTCCTC
    ACCGGTGACC TTATGAAGCT CATGGAGAGC TATAAGAATG GAGGGAGCTT ACTAATTCAG
    GGACCAGGGC ACTGTTCTCT CCTTCACTAC GCAGCTAAGA CCGGCAATGG GGACATTGTG
    AAGTACATCC TTGACCACGG ACCTGCAGAA TTACTGGATA TGGCAGACAG TGAAACGGGC
    GAGACTGCAC TGCACAAGGC TGCCTGCCAA AGGAACCGGG CTGTATGCCA GCTTCTGGTG
    GATGCAGGAG CATCTCTGAG ACAGACAGAC TCCAAGGGCA AGACACCCCA GGAACGAGCA
    CAGCAGGCCG GGGACCCAGA TTTGGCTGCT TACCTAGAAA GCCGACAGAA CTATAAGATC
    ATTGGCCATG AAGACCTGGA AACTGCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334033.1
    CDS29..3181
    Translation

    Target ORF information:

    RefSeq Version NM_001347104.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347104.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGCAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GATCCCCAGT CTGTCCCAGA TCGCCTGAGG
    ATCCGTGTGA ACAAAATCAG TTTACAAGAC TATGAAGGAC TCCACTATGA CAAGGACAAA
    CTCCGGGAAG CTTCCATCCC TCTGGGTATT CTAGTTGTTC GTGGAGACTG TGATTTGGAG
    ACTTGCCGTA TGTACATAGA CCGTCTACAG GAGGACTTGC AGTCAGTTTC TTCTGGGTCA
    CAGAGAGTTC ATTACCAGGA CCAAGAAACC TCCTTCCCCA GGGCACTCTC AGCTCAGAGG
    CTGTCCCCAC GGTGGTGCTT CCTAGATGCA ACGTCTGCTG ACCGCTTTTA TCGAATAGAC
    AGATCTCAGG AACATTTGCA CTTTGTGATG GAGATTTCTC ACGATGAAAT TTTTATTCTG
    GACCCAGATA TGGTGGTGTC ACAGCAGGCA GGGACCCCTC CAGGAATGCC TGACCTGGTG
    GTTGAACAGG CTTCTGGGCT GTCTGACTGG TGGAACCCTG CCCTGAGGAA ACGTATGCTG
    AGTGACAGTG GGATGATCAC ACCCCACTAT GAGGACTCAG ATCTGAAAGA CTTCAGCCAC
    TCCAGAGTGC TACAGTCCCC AGTCTCTTCA GAAGATCATG CAATTTTGCA GGCAGTCCTC
    ACCGGTGACC TTATGAAGCT CATGGAGAGC TATAAGAATG GAGGGAGCTT ACTAATTCAG
    GGACCAGGGC ACTGTTCTCT CCTTCACTAC GCAGCTAAGA CCGGCAATGG GGACATTGTG
    AAGTACATCC TTGACCACGG ACCTGCAGAA TTACTGGATA TGGCAGACAG TGAAACGGGC
    GAGACTGCAC TGCACAAGGC TGCCTGCCAA AGGAACCGGG CTGTATGCCA GCTTCTGGTG
    GATGCAGGAG CATCTCTGAG ACAGACAGAC TCCAAGGGCA AGACACCCCA GGAACGAGCA
    CAGCAGGCCG GGGACCCAGA TTTGGCTGCT TACCTAGAAA GCCGACAGAA CTATAAGATC
    ATTGGCCATG AAGACCTGGA AACTGCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37360
    Clone ID Related Accession (Same CDS sequence) XM_006506257.4 , XM_006506257.3
    Accession Version XM_006506257.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGC TCTCACCACT GGGTCAAACA TCTATTATAT GAACTCCCTG
    AAGGCTTCCA ACCGGAAGAA GAAGAGGACA AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA
    ACTGAGCAGG AAACCAAAGG CCGTCCTTTT GTGATCAAGC CCATCTCCTC CCCTCTCATG
    AAGCCTTTGC TTGTCTTTGT GAACCCAAAG AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA
    CAGATGTTTA TGTGGTACCT GAATCCACGG CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA
    AAAGATGCGC TGGAGATGTA CAGGAAGGTA CCAAATCTGA GAATTCTGGC CTGCGGTGGG
    GATGGAACGG TGGGCTGGAT CCTCTCCATC CTGGACGAGC TGCAGCTGAG CCCACAGCCT
    CCTGTGGGGG TGCTTCCACT GGGGACTGGG AATGACCTGG CTCGGACGCT CAACTGGGGA
    GGGGGTTACA CCGATGAGCC TGTTTCTAAG ATCCTGTGCC AGGTGGAAGA TGGGACCATT
    GTACAGTTGG ATCGCTGGAA CCTCCACGTA GAGAGAAACC CAGACCTGCC GCCCGAAGAA
    CTTGAAGATG GCGTGTGTAA GCTTCCTCTG AATGTCTTCA ATAATTACTT CAGCCTTGGA
    TTTGATGCTC ACGTCACATT GGAGTTCCAT GAATCCCGAG AAGCAAATCC AGAGAAATTC
    AATAGTCGTT TTCGAAATAA AATGTTCTAT GCAGGGGCAG CCTTTTCTGA TTTCCTACAA
    AGAAGTTCTA GAGATCTATC CAAACACGTC AAAGTTGTCT GTGATGGGAC AGACCTCACC
    CCAAAGATCC AGGACCTGAA GTTCCAGTGT ATAGTATTTT TAAATATTCC CAGATATTGT
    GCTGGCACAA TGCCTTGGGG AAACCCTGGA GATCATCATG ACTTTGAACC CCAACGTCAT
    GATGACGGCT ACATTGAAGT CATTGGATTC ACTATGGCCT CTTTGGCTGC CCTGCAAGTT
    GGGGGGCACG GAGAAAGGCT GCACCAGTGT CGGGAAGTGA TGCTTCTGAC TTACAAGTCC
    ATTCCCATGC AAGTGGACGG GGAGCCATGT CGGTTGGCAC CTGCTATGAT TCGGATCTCC
    TTGAGGAATC AGGCCAACAT GGTACAGAAG AGCAAGCGGA GAACCTCGAT GCCTTTGCTC
    AATGACATCC ATCAGGTGCA AGCTGCGGAC CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC
    TTCACCATTC CCCAGTCTGT CCCAGATCGC CTGAGGATCC GTGTGAACAA AATCAGTTTA
    CAAGACTATG AAGGACTCCA CTATGACAAG GACAAACTCC GGGAAGCTTC CATCCCTCTG
    GGTATTCTAG TTGTTCGTGG AGACTGTGAT TTGGAGACTT GCCGTATGTA CATAGACCGT
    CTACAGGAGG ACTTGCAGTC AGTTTCTTCT GGGTCACAGA GAGTTCATTA CCAGGACCAA
    GAAACCTCCT TCCCCAGGGC ACTCTCAGCT CAGAGGCTGT CCCCACGGTG GTGCTTCCTA
    GATGCAACGT CTGCTGACCG CTTTTATCGA ATAGACAGAT CTCAGGAACA TTTGCACTTT
    GTGATGGAGA TTTCTCACGA TGAAATTTTT ATTCTGGACC CAGATATGGT GGTGTCACAG
    CAGGCAGGGA CCCCTCCAGG AATGCCTGAC CTGGTGGTTG AACAGGCTTC TGGGCTGTCT
    GACTGGTGGA ACCCTGCCCT GAGGAAACGT ATGCTGAGTG ACAGTGGGAT GATCACACCC
    CACTATGAGG ACTCAGATCT GAAAGACTTC AGCCACTCCA GAGTGCTACA GTCCCCAGTC
    TCTTCAGAAG ATCATGCAAT TTTGCAGGCA GTCCTCACCG GTGACCTTAT GAAGCTCATG
    GAGAGCTATA AGAATGGAGG GAGCTTACTA ATTCAGGGAC CAGGGCACTG TTCTCTCCTT
    CACTACGCAG CTAAGACCGG CAATGGGGAC ATTGTGAAGT ACATCCTTGA CCACGGACCT
    GCAGAATTAC TGGATATGGC AGACAGTGAA ACGGGCGAGA CTGCACTGCA CAAGGCTGCC
    TGCCAAAGGA ACCGGGCTGT ATGCCAGCTT CTGGTGGATG CAGGAGCATC TCTGAGACAG
    ACAGACTCCA AGGGCAAGAC ACCCCAGGAA CGAGCACAGC AGGCCGGGGA CCCAGATTTG
    GCTGCTTACC TAGAAAGCCG ACAGAACTAT AAGATCATTG GCCATGAAGA CCTGGAAACT
    GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506320.1
    CDS112..3360
    Translation

    Target ORF information:

    RefSeq Version XM_006506257.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506257.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGC TCTCACCACT GGGTCAAACA TCTATTATAT GAACTCCCTG
    AAGGCTTCCA ACCGGAAGAA GAAGAGGACA AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA
    ACTGAGCAGG AAACCAAAGG CCGTCCTTTT GTGATCAAGC CCATCTCCTC CCCTCTCATG
    AAGCCTTTGC TTGTCTTTGT GAACCCAAAG AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA
    CAGATGTTTA TGTGGTACCT GAATCCACGG CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA
    AAAGATGCGC TGGAGATGTA CAGGAAGGTA CCAAATCTGA GAATTCTGGC CTGCGGTGGG
    GATGGAACGG TGGGCTGGAT CCTCTCCATC CTGGACGAGC TGCAGCTGAG CCCACAGCCT
    CCTGTGGGGG TGCTTCCACT GGGGACTGGG AATGACCTGG CTCGGACGCT CAACTGGGGA
    GGGGGTTACA CCGATGAGCC TGTTTCTAAG ATCCTGTGCC AGGTGGAAGA TGGGACCATT
    GTACAGTTGG ATCGCTGGAA CCTCCACGTA GAGAGAAACC CAGACCTGCC GCCCGAAGAA
    CTTGAAGATG GCGTGTGTAA GCTTCCTCTG AATGTCTTCA ATAATTACTT CAGCCTTGGA
    TTTGATGCTC ACGTCACATT GGAGTTCCAT GAATCCCGAG AAGCAAATCC AGAGAAATTC
    AATAGTCGTT TTCGAAATAA AATGTTCTAT GCAGGGGCAG CCTTTTCTGA TTTCCTACAA
    AGAAGTTCTA GAGATCTATC CAAACACGTC AAAGTTGTCT GTGATGGGAC AGACCTCACC
    CCAAAGATCC AGGACCTGAA GTTCCAGTGT ATAGTATTTT TAAATATTCC CAGATATTGT
    GCTGGCACAA TGCCTTGGGG AAACCCTGGA GATCATCATG ACTTTGAACC CCAACGTCAT
    GATGACGGCT ACATTGAAGT CATTGGATTC ACTATGGCCT CTTTGGCTGC CCTGCAAGTT
    GGGGGGCACG GAGAAAGGCT GCACCAGTGT CGGGAAGTGA TGCTTCTGAC TTACAAGTCC
    ATTCCCATGC AAGTGGACGG GGAGCCATGT CGGTTGGCAC CTGCTATGAT TCGGATCTCC
    TTGAGGAATC AGGCCAACAT GGTACAGAAG AGCAAGCGGA GAACCTCGAT GCCTTTGCTC
    AATGACATCC ATCAGGTGCA AGCTGCGGAC CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC
    TTCACCATTC CCCAGTCTGT CCCAGATCGC CTGAGGATCC GTGTGAACAA AATCAGTTTA
    CAAGACTATG AAGGACTCCA CTATGACAAG GACAAACTCC GGGAAGCTTC CATCCCTCTG
    GGTATTCTAG TTGTTCGTGG AGACTGTGAT TTGGAGACTT GCCGTATGTA CATAGACCGT
    CTACAGGAGG ACTTGCAGTC AGTTTCTTCT GGGTCACAGA GAGTTCATTA CCAGGACCAA
    GAAACCTCCT TCCCCAGGGC ACTCTCAGCT CAGAGGCTGT CCCCACGGTG GTGCTTCCTA
    GATGCAACGT CTGCTGACCG CTTTTATCGA ATAGACAGAT CTCAGGAACA TTTGCACTTT
    GTGATGGAGA TTTCTCACGA TGAAATTTTT ATTCTGGACC CAGATATGGT GGTGTCACAG
    CAGGCAGGGA CCCCTCCAGG AATGCCTGAC CTGGTGGTTG AACAGGCTTC TGGGCTGTCT
    GACTGGTGGA ACCCTGCCCT GAGGAAACGT ATGCTGAGTG ACAGTGGGAT GATCACACCC
    CACTATGAGG ACTCAGATCT GAAAGACTTC AGCCACTCCA GAGTGCTACA GTCCCCAGTC
    TCTTCAGAAG ATCATGCAAT TTTGCAGGCA GTCCTCACCG GTGACCTTAT GAAGCTCATG
    GAGAGCTATA AGAATGGAGG GAGCTTACTA ATTCAGGGAC CAGGGCACTG TTCTCTCCTT
    CACTACGCAG CTAAGACCGG CAATGGGGAC ATTGTGAAGT ACATCCTTGA CCACGGACCT
    GCAGAATTAC TGGATATGGC AGACAGTGAA ACGGGCGAGA CTGCACTGCA CAAGGCTGCC
    TGCCAAAGGA ACCGGGCTGT ATGCCAGCTT CTGGTGGATG CAGGAGCATC TCTGAGACAG
    ACAGACTCCA AGGGCAAGAC ACCCCAGGAA CGAGCACAGC AGGCCGGGGA CCCAGATTTG
    GCTGCTTACC TAGAAAGCCG ACAGAACTAT AAGATCATTG GCCATGAAGA CCTGGAAACT
    GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37361
    Clone ID Related Accession (Same CDS sequence) XM_006506258.4 , XM_006506258.3
    Accession Version XM_006506258.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGGAAACCA AAGGCCGTCC TTTTGTGATC
    AAGCCCATCT CCTCCCCTCT CATGAAGCCT TTGCTTGTCT TTGTGAACCC AAAGAGTGGG
    GGAAACCAGG GAACCAAAGT CCTACAGATG TTTATGTGGT ACCTGAATCC ACGGCAAGTC
    TTTGATCTTT CTCAGGAAGG GCCAAAAGAT GCGCTGGAGA TGTACAGGAA GGTACCAAAT
    CTGAGAATTC TGGCCTGCGG TGGGGATGGA ACGGTGGGCT GGATCCTCTC CATCCTGGAC
    GAGCTGCAGC TGAGCCCACA GCCTCCTGTG GGGGTGCTTC CACTGGGGAC TGGGAATGAC
    CTGGCTCGGA CGCTCAACTG GGGAGGGGGT TACACCGATG AGCCTGTTTC TAAGATCCTG
    TGCCAGGTGG AAGATGGGAC CATTGTACAG TTGGATCGCT GGAACCTCCA CGTAGAGAGA
    AACCCAGACC TGCCGCCCGA AGAACTTGAA GATGGCGTGT GTAAGCTTCC TCTGAATGTC
    TTCAATAATT ACTTCAGCCT TGGATTTGAT GCTCACGTCA CATTGGAGTT CCATGAATCC
    CGAGAAGCAA ATCCAGAGAA ATTCAATAGT CGTTTTCGAA ATAAAATGTT CTATGCAGGG
    GCAGCCTTTT CTGATTTCCT ACAAAGAAGT TCTAGAGATC TATCCAAACA CGTCAAAGTT
    GTCTGTGATG GGACAGACCT CACCCCAAAG ATCCAGGACC TGAAGTTCCA GTGTATAGTA
    TTTTTAAATA TTCCCAGATA TTGTGCTGGC ACAATGCCTT GGGGAAACCC TGGAGATCAT
    CATGACTTTG AACCCCAACG TCATGATGAC GGCTACATTG AAGTCATTGG ATTCACTATG
    GCCTCTTTGG CTGCCCTGCA AGTTGGGGGG CACGGAGAAA GGCTGCACCA GTGTCGGGAA
    GTGATGCTTC TGACTTACAA GTCCATTCCC ATGCAAGTGG ACGGGGAGCC ATGTCGGTTG
    GCACCTGCTA TGATTCGGAT CTCCTTGAGG AATCAGGCCA ACATGGTACA GAAGAGCAAG
    CGGAGAACCT CGATGCCTTT GCTCAATGAC ATCCATCAGG TGCAAGCTGC GGACCTGCGG
    CGAGTGTCTG CTCCCCCCGG CTCCTTCACC ATTCCCCAGT CTGTCCCAGA TCGCCTGAGG
    ATCCGTGTGA ACAAAATCAG TTTACAAGAC TATGAAGGAC TCCACTATGA CAAGGACAAA
    CTCCGGGAAG CTTCCATCCC TCTGGGTATT CTAGTTGTTC GTGGAGACTG TGATTTGGAG
    ACTTGCCGTA TGTACATAGA CCGTCTACAG GAGGACTTGC AGTCAGTTTC TTCTGGGTCA
    CAGAGAGTTC ATTACCAGGA CCAAGAAACC TCCTTCCCCA GGGCACTCTC AGCTCAGAGG
    CTGTCCCCAC GGTGGTGCTT CCTAGATGCA ACGTCTGCTG ACCGCTTTTA TCGAATAGAC
    AGATCTCAGG AACATTTGCA CTTTGTGATG GAGATTTCTC ACGATGAAAT TTTTATTCTG
    GACCCAGATA TGGTGGTGTC ACAGCAGGCA GGGACCCCTC CAGGAATGCC TGACCTGGTG
    GTTGAACAGG CTTCTGGGCT GTCTGACTGG TGGAACCCTG CCCTGAGGAA ACGTATGCTG
    AGTGACAGTG GGATGATCAC ACCCCACTAT GAGGACTCAG ATCTGAAAGA CTTCAGCCAC
    TCCAGAGTGC TACAGTCCCC AGTCTCTTCA GAAGATCATG CAATTTTGCA GGCAGTCCTC
    ACCGGTGACC TTATGAAGCT CATGGAGAGC TATAAGAATG GAGGGAGCTT ACTAATTCAG
    GGACCAGGGC ACTGTTCTCT CCTTCACTAC GCAGCTAAGA CCGGCAATGG GGACATTGTG
    AAGTACATCC TTGACCACGG ACCTGCAGAA TTACTGGATA TGGCAGACAG TGAAACGGGC
    GAGACTGCAC TGCACAAGGC TGCCTGCCAA AGGAACCGGG CTGTATGCCA GCTTCTGGTG
    GATGCAGGAG CATCTCTGAG ACAGACAGAC TCCAAGGGCA AGACACCCCA GGAACGAGCA
    CAGCAGGCCG GGGACCCAGA TTTGGCTGCT TACCTAGAAA GCCGACAGAA CTATAAGATC
    ATTGGCCATG AAGACCTGGA AACTGCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506321.1
    CDS112..3324
    Translation

    Target ORF information:

    RefSeq Version XM_006506258.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506258.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGGAAACCA AAGGCCGTCC TTTTGTGATC
    AAGCCCATCT CCTCCCCTCT CATGAAGCCT TTGCTTGTCT TTGTGAACCC AAAGAGTGGG
    GGAAACCAGG GAACCAAAGT CCTACAGATG TTTATGTGGT ACCTGAATCC ACGGCAAGTC
    TTTGATCTTT CTCAGGAAGG GCCAAAAGAT GCGCTGGAGA TGTACAGGAA GGTACCAAAT
    CTGAGAATTC TGGCCTGCGG TGGGGATGGA ACGGTGGGCT GGATCCTCTC CATCCTGGAC
    GAGCTGCAGC TGAGCCCACA GCCTCCTGTG GGGGTGCTTC CACTGGGGAC TGGGAATGAC
    CTGGCTCGGA CGCTCAACTG GGGAGGGGGT TACACCGATG AGCCTGTTTC TAAGATCCTG
    TGCCAGGTGG AAGATGGGAC CATTGTACAG TTGGATCGCT GGAACCTCCA CGTAGAGAGA
    AACCCAGACC TGCCGCCCGA AGAACTTGAA GATGGCGTGT GTAAGCTTCC TCTGAATGTC
    TTCAATAATT ACTTCAGCCT TGGATTTGAT GCTCACGTCA CATTGGAGTT CCATGAATCC
    CGAGAAGCAA ATCCAGAGAA ATTCAATAGT CGTTTTCGAA ATAAAATGTT CTATGCAGGG
    GCAGCCTTTT CTGATTTCCT ACAAAGAAGT TCTAGAGATC TATCCAAACA CGTCAAAGTT
    GTCTGTGATG GGACAGACCT CACCCCAAAG ATCCAGGACC TGAAGTTCCA GTGTATAGTA
    TTTTTAAATA TTCCCAGATA TTGTGCTGGC ACAATGCCTT GGGGAAACCC TGGAGATCAT
    CATGACTTTG AACCCCAACG TCATGATGAC GGCTACATTG AAGTCATTGG ATTCACTATG
    GCCTCTTTGG CTGCCCTGCA AGTTGGGGGG CACGGAGAAA GGCTGCACCA GTGTCGGGAA
    GTGATGCTTC TGACTTACAA GTCCATTCCC ATGCAAGTGG ACGGGGAGCC ATGTCGGTTG
    GCACCTGCTA TGATTCGGAT CTCCTTGAGG AATCAGGCCA ACATGGTACA GAAGAGCAAG
    CGGAGAACCT CGATGCCTTT GCTCAATGAC ATCCATCAGG TGCAAGCTGC GGACCTGCGG
    CGAGTGTCTG CTCCCCCCGG CTCCTTCACC ATTCCCCAGT CTGTCCCAGA TCGCCTGAGG
    ATCCGTGTGA ACAAAATCAG TTTACAAGAC TATGAAGGAC TCCACTATGA CAAGGACAAA
    CTCCGGGAAG CTTCCATCCC TCTGGGTATT CTAGTTGTTC GTGGAGACTG TGATTTGGAG
    ACTTGCCGTA TGTACATAGA CCGTCTACAG GAGGACTTGC AGTCAGTTTC TTCTGGGTCA
    CAGAGAGTTC ATTACCAGGA CCAAGAAACC TCCTTCCCCA GGGCACTCTC AGCTCAGAGG
    CTGTCCCCAC GGTGGTGCTT CCTAGATGCA ACGTCTGCTG ACCGCTTTTA TCGAATAGAC
    AGATCTCAGG AACATTTGCA CTTTGTGATG GAGATTTCTC ACGATGAAAT TTTTATTCTG
    GACCCAGATA TGGTGGTGTC ACAGCAGGCA GGGACCCCTC CAGGAATGCC TGACCTGGTG
    GTTGAACAGG CTTCTGGGCT GTCTGACTGG TGGAACCCTG CCCTGAGGAA ACGTATGCTG
    AGTGACAGTG GGATGATCAC ACCCCACTAT GAGGACTCAG ATCTGAAAGA CTTCAGCCAC
    TCCAGAGTGC TACAGTCCCC AGTCTCTTCA GAAGATCATG CAATTTTGCA GGCAGTCCTC
    ACCGGTGACC TTATGAAGCT CATGGAGAGC TATAAGAATG GAGGGAGCTT ACTAATTCAG
    GGACCAGGGC ACTGTTCTCT CCTTCACTAC GCAGCTAAGA CCGGCAATGG GGACATTGTG
    AAGTACATCC TTGACCACGG ACCTGCAGAA TTACTGGATA TGGCAGACAG TGAAACGGGC
    GAGACTGCAC TGCACAAGGC TGCCTGCCAA AGGAACCGGG CTGTATGCCA GCTTCTGGTG
    GATGCAGGAG CATCTCTGAG ACAGACAGAC TCCAAGGGCA AGACACCCCA GGAACGAGCA
    CAGCAGGCCG GGGACCCAGA TTTGGCTGCT TACCTAGAAA GCCGACAGAA CTATAAGATC
    ATTGGCCATG AAGACCTGGA AACTGCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu37362
    Clone ID Related Accession (Same CDS sequence) XM_006506259.3 , XM_006506259.4
    Accession Version XM_006506259.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506259.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGAACTCCC TGAAGGCTTC CAACCGGAAG AAGAAGAGGA CAAGTTTTAA AAGGAAAGCC
    AGTAAAAGAG GAACTGAGCA GGAAACCAAA GGCCGTCCTT TTGTGATCAA GCCCATCTCC
    TCCCCTCTCA TGAAGCCTTT GCTTGTCTTT GTGAACCCAA AGAGTGGGGG AAACCAGGGA
    ACCAAAGTCC TACAGATGTT TATGTGGTAC CTGAATCCAC GGCAAGTCTT TGATCTTTCT
    CAGGAAGGGC CAAAAGATGC GCTGGAGATG TACAGGAAGG TACCAAATCT GAGAATTCTG
    GCCTGCGGTG GGGATGGAAC GGTGGGCTGG ATCCTCTCCA TCCTGGACGA GCTGCAGCTG
    AGCCCACAGC CTCCTGTGGG GGTGCTTCCA CTGGGGACTG GGAATGACCT GGCTCGGACG
    CTCAACTGGG GAGGGGGTTA CACCGATGAG CCTGTTTCTA AGATCCTGTG CCAGGTGGAA
    GATGGGACCA TTGTACAGTT GGATCGCTGG AACCTCCACG TAGAGAGAAA CCCAGACCTG
    CCGCCCGAAG AACTTGAAGA TGGCGTGTGT AAGCTTCCTC TGAATGTCTT CAATAATTAC
    TTCAGCCTTG GATTTGATGC TCACGTCACA TTGGAGTTCC ATGAATCCCG AGAAGCAAAT
    CCAGAGAAAT TCAATAGTCG TTTTCGAAAT AAAATGTTCT ATGCAGGGGC AGCCTTTTCT
    GATTTCCTAC AAAGAAGTTC TAGAGATCTA TCCAAACACG TCAAAGTTGT CTGTGATGGG
    ACAGACCTCA CCCCAAAGAT CCAGGACCTG AAGTTCCAGT GTATAGTATT TTTAAATATT
    CCCAGATATT GTGCTGGCAC AATGCCTTGG GGAAACCCTG GAGATCATCA TGACTTTGAA
    CCCCAACGTC ATGATGACGG CTACATTGAA GTCATTGGAT TCACTATGGC CTCTTTGGCT
    GCCCTGCAAG TTGGGGGGCA CGGAGAAAGG CTGCACCAGT GTCGGGAAGT GATGCTTCTG
    ACTTACAAGT CCATTCCCAT GCAAGTGGAC GGGGAGCCAT GTCGGTTGGC ACCTGCTATG
    ATTCGGATCT CCTTGAGGAA TCAGGCCAAC ATGGTACAGA AGAGCAAGCG GAGAACCTCG
    ATGCCTTTGC TCAATGACAT CCATCAGGTG CAAGCTGCGG ACCTGCGGCG AGTGTCTGCT
    CCCCCCGGCT CCTTCACCAT TCCCCAGTCT GTCCCAGATC GCCTGAGGAT CCGTGTGAAC
    AAAATCAGTT TACAAGACTA TGAAGGACTC CACTATGACA AGGACAAACT CCGGGAAGCT
    TCCATCCCTC TGGGTATTCT AGTTGTTCGT GGAGACTGTG ATTTGGAGAC TTGCCGTATG
    TACATAGACC GTCTACAGGA GGACTTGCAG TCAGTTTCTT CTGGGTCACA GAGAGTTCAT
    TACCAGGACC AAGAAACCTC CTTCCCCAGG GCACTCTCAG CTCAGAGGCT GTCCCCACGG
    TGGTGCTTCC TAGATGCAAC GTCTGCTGAC CGCTTTTATC GAATAGACAG ATCTCAGGAA
    CATTTGCACT TTGTGATGGA GATTTCTCAC GATGAAATTT TTATTCTGGA CCCAGATATG
    GTGGTGTCAC AGCAGGCAGG GACCCCTCCA GGAATGCCTG ACCTGGTGGT TGAACAGGCT
    TCTGGGCTGT CTGACTGGTG GAACCCTGCC CTGAGGAAAC GTATGCTGAG TGACAGTGGG
    ATGATCACAC CCCACTATGA GGACTCAGAT CTGAAAGACT TCAGCCACTC CAGAGTGCTA
    CAGTCCCCAG TCTCTTCAGA AGATCATGCA ATTTTGCAGG CAGTCCTCAC CGGTGACCTT
    ATGAAGCTCA TGGAGAGCTA TAAGAATGGA GGGAGCTTAC TAATTCAGGG ACCAGGGCAC
    TGTTCTCTCC TTCACTACGC AGCTAAGACC GGCAATGGGG ACATTGTGAA GTACATCCTT
    GACCACGGAC CTGCAGAATT ACTGGATATG GCAGACAGTG AAACGGGCGA GACTGCACTG
    CACAAGGCTG CCTGCCAAAG GAACCGGGCT GTATGCCAGC TTCTGGTGGA TGCAGGAGCA
    TCTCTGAGAC AGACAGACTC CAAGGGCAAG ACACCCCAGG AACGAGCACA GCAGGCCGGG
    GACCCAGATT TGGCTGCTTA CCTAGAAAGC CGACAGAACT ATAAGATCAT TGGCCATGAA
    GACCTGGAAA CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006506322.1
    CDS112..3312
    Translation

    Target ORF information:

    RefSeq Version XM_006506259.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006506259.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGAACTCCC TGAAGGCTTC CAACCGGAAG AAGAAGAGGA CAAGTTTTAA AAGGAAAGCC
    AGTAAAAGAG GAACTGAGCA GGAAACCAAA GGCCGTCCTT TTGTGATCAA GCCCATCTCC
    TCCCCTCTCA TGAAGCCTTT GCTTGTCTTT GTGAACCCAA AGAGTGGGGG AAACCAGGGA
    ACCAAAGTCC TACAGATGTT TATGTGGTAC CTGAATCCAC GGCAAGTCTT TGATCTTTCT
    CAGGAAGGGC CAAAAGATGC GCTGGAGATG TACAGGAAGG TACCAAATCT GAGAATTCTG
    GCCTGCGGTG GGGATGGAAC GGTGGGCTGG ATCCTCTCCA TCCTGGACGA GCTGCAGCTG
    AGCCCACAGC CTCCTGTGGG GGTGCTTCCA CTGGGGACTG GGAATGACCT GGCTCGGACG
    CTCAACTGGG GAGGGGGTTA CACCGATGAG CCTGTTTCTA AGATCCTGTG CCAGGTGGAA
    GATGGGACCA TTGTACAGTT GGATCGCTGG AACCTCCACG TAGAGAGAAA CCCAGACCTG
    CCGCCCGAAG AACTTGAAGA TGGCGTGTGT AAGCTTCCTC TGAATGTCTT CAATAATTAC
    TTCAGCCTTG GATTTGATGC TCACGTCACA TTGGAGTTCC ATGAATCCCG AGAAGCAAAT
    CCAGAGAAAT TCAATAGTCG TTTTCGAAAT AAAATGTTCT ATGCAGGGGC AGCCTTTTCT
    GATTTCCTAC AAAGAAGTTC TAGAGATCTA TCCAAACACG TCAAAGTTGT CTGTGATGGG
    ACAGACCTCA CCCCAAAGAT CCAGGACCTG AAGTTCCAGT GTATAGTATT TTTAAATATT
    CCCAGATATT GTGCTGGCAC AATGCCTTGG GGAAACCCTG GAGATCATCA TGACTTTGAA
    CCCCAACGTC ATGATGACGG CTACATTGAA GTCATTGGAT TCACTATGGC CTCTTTGGCT
    GCCCTGCAAG TTGGGGGGCA CGGAGAAAGG CTGCACCAGT GTCGGGAAGT GATGCTTCTG
    ACTTACAAGT CCATTCCCAT GCAAGTGGAC GGGGAGCCAT GTCGGTTGGC ACCTGCTATG
    ATTCGGATCT CCTTGAGGAA TCAGGCCAAC ATGGTACAGA AGAGCAAGCG GAGAACCTCG
    ATGCCTTTGC TCAATGACAT CCATCAGGTG CAAGCTGCGG ACCTGCGGCG AGTGTCTGCT
    CCCCCCGGCT CCTTCACCAT TCCCCAGTCT GTCCCAGATC GCCTGAGGAT CCGTGTGAAC
    AAAATCAGTT TACAAGACTA TGAAGGACTC CACTATGACA AGGACAAACT CCGGGAAGCT
    TCCATCCCTC TGGGTATTCT AGTTGTTCGT GGAGACTGTG ATTTGGAGAC TTGCCGTATG
    TACATAGACC GTCTACAGGA GGACTTGCAG TCAGTTTCTT CTGGGTCACA GAGAGTTCAT
    TACCAGGACC AAGAAACCTC CTTCCCCAGG GCACTCTCAG CTCAGAGGCT GTCCCCACGG
    TGGTGCTTCC TAGATGCAAC GTCTGCTGAC CGCTTTTATC GAATAGACAG ATCTCAGGAA
    CATTTGCACT TTGTGATGGA GATTTCTCAC GATGAAATTT TTATTCTGGA CCCAGATATG
    GTGGTGTCAC AGCAGGCAGG GACCCCTCCA GGAATGCCTG ACCTGGTGGT TGAACAGGCT
    TCTGGGCTGT CTGACTGGTG GAACCCTGCC CTGAGGAAAC GTATGCTGAG TGACAGTGGG
    ATGATCACAC CCCACTATGA GGACTCAGAT CTGAAAGACT TCAGCCACTC CAGAGTGCTA
    CAGTCCCCAG TCTCTTCAGA AGATCATGCA ATTTTGCAGG CAGTCCTCAC CGGTGACCTT
    ATGAAGCTCA TGGAGAGCTA TAAGAATGGA GGGAGCTTAC TAATTCAGGG ACCAGGGCAC
    TGTTCTCTCC TTCACTACGC AGCTAAGACC GGCAATGGGG ACATTGTGAA GTACATCCTT
    GACCACGGAC CTGCAGAATT ACTGGATATG GCAGACAGTG AAACGGGCGA GACTGCACTG
    CACAAGGCTG CCTGCCAAAG GAACCGGGCT GTATGCCAGC TTCTGGTGGA TGCAGGAGCA
    TCTCTGAGAC AGACAGACTC CAAGGGCAAG ACACCCCAGG AACGAGCACA GCAGGCCGGG
    GACCCAGATT TGGCTGCTTA CCTAGAAAGC CGACAGAACT ATAAGATCAT TGGCCATGAA
    GACCTGGAAA CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74609
    Clone ID Related Accession (Same CDS sequence) XM_011241385.2 , XM_011241385.3
    Accession Version XM_011241385.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGGAAACCA AAGGCCGTCC TTTTGTGATC
    AAGCCCATCT CCTCCCCTCT CATGAAGCCT TTGCTTGTCT TTGTGAACCC AAAGAGTGGG
    GGAAACCAGG GAACCAAAGT CCTACAGATG TTTATGTGGT ACCTGAATCC ACGGCAAGTC
    TTTGATCTTT CTCAGGAAGG GCCAAAAGAT GCGCTGGAGA TGTACAGGAA GGTACCAAAT
    CTGAGAATTC TGGCCTGCGG TGGGGATGGA ACGGTGGGCT GGATCCTCTC CATCCTGGAC
    GAGCTGCAGC TGAGCCCACA GCCTCCTGTG GGGGTGCTTC CACTGGGGAC TGGGAATGAC
    CTGGCTCGGA CGCTCAACTG GGGAGGGGGT TACACCGATG AGCCTGTTTC TAAGATCCTG
    TGCCAGGTGG AAGATGGGAC CATTGTACAG TTGGATCGCT GGAACCTCCA CGTAGAGAGA
    AACCCAGACC TGCCGCCCGA AGAACTTGAA GATGGCGTGT GTAAGCTTCC TCTGAATGTC
    TTCAATAATT ACTTCAGCCT TGGATTTGAT GCTCACGTCA CATTGGAGTT CCATGAATCC
    CGAGAAGCAA ATCCAGAGAA ATTCAATAGT CGTTTTCGAA ATAAAATGTT CTATGCAGGG
    GCAGCCTTTT CTGATTTCCT ACAAAGAAGT TCTAGAGATC TATCCAAACA CGTCAAAGTT
    GTCTGTGATG GGACAGACCT CACCCCAAAG ATCCAGGACC TGAAGTTCCA GTGTATAGTA
    TTTTTAAATA TTCCCAGATA TTGTGCTGGC ACAATGCCTT GGGGAAACCC TGGAGATCAT
    CATGACTTTG AACCCCAACG TCATGATGAC GGCTACATTG AAGTCATTGG ATTCACTATG
    GCCTCTTTGG CTGCCCTGCA AGTTGGGGGG CACGGAGAAA GGCTGCACCA GTGTCGGGAA
    GTGATGCTTC TGACTTACAA GTCCATTCCC ATGCAAGTGG ACGGGGAGCC ATGTCGGTTG
    GCACCTGCTA TGATTCGGAT CTCCTTGAGG AATCAGGCCA ACATGGTACA GAAGAGCAAG
    CGGAGAACCT CGATGCCTTT GCTCAATGAT CCCCAGTCTG TCCCAGATCG CCTGAGGATC
    CGTGTGAACA AAATCAGTTT ACAAGACTAT GAAGGACTCC ACTATGACAA GGACAAACTC
    CGGGAAGCTT CCATCCCTCT GGGTATTCTA GTTGTTCGTG GAGACTGTGA TTTGGAGACT
    TGCCGTATGT ACATAGACCG TCTACAGGAG GACCAAGAAA CCTCCTTCCC CAGGGCACTC
    TCAGCTCAGA GGCTGTCCCC ACGGTGGTGC TTCCTAGATG CAACGTCTGC TGACCGCTTT
    TATCGAATAG ACAGATCTCA GGAACATTTG CACTTTGTGA TGGAGATTTC TCACGATGAA
    ATTTTTATTC TGGACCCAGA TATGGTGGTG TCACAGCAGG CAGGGACCCC TCCAGGAATG
    CCTGACCTGG TGGTTGAACA GGCTTCTGGG CTGTCTGACT GGTGGAACCC TGCCCTGAGG
    AAACGTATGC TGAGTGACAG TGGGATGATC ACACCCCACT ATGAGGACTC AGATCTGAAA
    GACTTCAGCC ACTCCAGAGT GCTACAGTCC CCAGTCTCTT CAGAAGATCA TGCAATTTTG
    CAGGCAGTCC TCACCGGTGA CCTTATGAAG CTCATGGAGA GCTATAAGAA TGGAGGGAGC
    TTACTAATTC AGGGACCAGG GCACTGTTCT CTCCTTCACT ACGCAGCTAA GACCGGCAAT
    GGGGACATTG TGAAGTACAT CCTTGACCAC GGACCTGCAG AATTACTGGA TATGGCAGAC
    AGTGAAACGG GCGAGACTGC ACTGCACAAG GCTGCCTGCC AAAGGAACCG GGCTGTATGC
    CAGCTTCTGG TGGATGCAGG AGCATCTCTG AGACAGACAG ACTCCAAGGG CAAGACACCC
    CAGGAACGAG CACAGCAGGC CGGGGACCCA GATTTGGCTG CTTACCTAGA AAGCCGACAG
    AACTATAAGA TCATTGGCCA TGAAGACCTG GAAACTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011239687.1
    CDS112..3216
    Translation

    Target ORF information:

    RefSeq Version XM_011241385.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011241385.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGAA CTCCCTGAAG GCTTCCAACC GGAAGAAGAA GAGGACAAGT
    TTTAAAAGGA AAGCCAGTAA AAGAGGAACT GAGGAAACCA AAGGCCGTCC TTTTGTGATC
    AAGCCCATCT CCTCCCCTCT CATGAAGCCT TTGCTTGTCT TTGTGAACCC AAAGAGTGGG
    GGAAACCAGG GAACCAAAGT CCTACAGATG TTTATGTGGT ACCTGAATCC ACGGCAAGTC
    TTTGATCTTT CTCAGGAAGG GCCAAAAGAT GCGCTGGAGA TGTACAGGAA GGTACCAAAT
    CTGAGAATTC TGGCCTGCGG TGGGGATGGA ACGGTGGGCT GGATCCTCTC CATCCTGGAC
    GAGCTGCAGC TGAGCCCACA GCCTCCTGTG GGGGTGCTTC CACTGGGGAC TGGGAATGAC
    CTGGCTCGGA CGCTCAACTG GGGAGGGGGT TACACCGATG AGCCTGTTTC TAAGATCCTG
    TGCCAGGTGG AAGATGGGAC CATTGTACAG TTGGATCGCT GGAACCTCCA CGTAGAGAGA
    AACCCAGACC TGCCGCCCGA AGAACTTGAA GATGGCGTGT GTAAGCTTCC TCTGAATGTC
    TTCAATAATT ACTTCAGCCT TGGATTTGAT GCTCACGTCA CATTGGAGTT CCATGAATCC
    CGAGAAGCAA ATCCAGAGAA ATTCAATAGT CGTTTTCGAA ATAAAATGTT CTATGCAGGG
    GCAGCCTTTT CTGATTTCCT ACAAAGAAGT TCTAGAGATC TATCCAAACA CGTCAAAGTT
    GTCTGTGATG GGACAGACCT CACCCCAAAG ATCCAGGACC TGAAGTTCCA GTGTATAGTA
    TTTTTAAATA TTCCCAGATA TTGTGCTGGC ACAATGCCTT GGGGAAACCC TGGAGATCAT
    CATGACTTTG AACCCCAACG TCATGATGAC GGCTACATTG AAGTCATTGG ATTCACTATG
    GCCTCTTTGG CTGCCCTGCA AGTTGGGGGG CACGGAGAAA GGCTGCACCA GTGTCGGGAA
    GTGATGCTTC TGACTTACAA GTCCATTCCC ATGCAAGTGG ACGGGGAGCC ATGTCGGTTG
    GCACCTGCTA TGATTCGGAT CTCCTTGAGG AATCAGGCCA ACATGGTACA GAAGAGCAAG
    CGGAGAACCT CGATGCCTTT GCTCAATGAT CCCCAGTCTG TCCCAGATCG CCTGAGGATC
    CGTGTGAACA AAATCAGTTT ACAAGACTAT GAAGGACTCC ACTATGACAA GGACAAACTC
    CGGGAAGCTT CCATCCCTCT GGGTATTCTA GTTGTTCGTG GAGACTGTGA TTTGGAGACT
    TGCCGTATGT ACATAGACCG TCTACAGGAG GACCAAGAAA CCTCCTTCCC CAGGGCACTC
    TCAGCTCAGA GGCTGTCCCC ACGGTGGTGC TTCCTAGATG CAACGTCTGC TGACCGCTTT
    TATCGAATAG ACAGATCTCA GGAACATTTG CACTTTGTGA TGGAGATTTC TCACGATGAA
    ATTTTTATTC TGGACCCAGA TATGGTGGTG TCACAGCAGG CAGGGACCCC TCCAGGAATG
    CCTGACCTGG TGGTTGAACA GGCTTCTGGG CTGTCTGACT GGTGGAACCC TGCCCTGAGG
    AAACGTATGC TGAGTGACAG TGGGATGATC ACACCCCACT ATGAGGACTC AGATCTGAAA
    GACTTCAGCC ACTCCAGAGT GCTACAGTCC CCAGTCTCTT CAGAAGATCA TGCAATTTTG
    CAGGCAGTCC TCACCGGTGA CCTTATGAAG CTCATGGAGA GCTATAAGAA TGGAGGGAGC
    TTACTAATTC AGGGACCAGG GCACTGTTCT CTCCTTCACT ACGCAGCTAA GACCGGCAAT
    GGGGACATTG TGAAGTACAT CCTTGACCAC GGACCTGCAG AATTACTGGA TATGGCAGAC
    AGTGAAACGG GCGAGACTGC ACTGCACAAG GCTGCCTGCC AAAGGAACCG GGCTGTATGC
    CAGCTTCTGG TGGATGCAGG AGCATCTCTG AGACAGACAG ACTCCAAGGG CAAGACACCC
    CAGGAACGAG CACAGCAGGC CGGGGACCCA GATTTGGCTG CTTACCTAGA AAGCCGACAG
    AACTATAAGA TCATTGGCCA TGAAGACCTG GAAACTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81748
    Clone ID Related Accession (Same CDS sequence) XM_017321624.1 , XM_017321624.2
    Accession Version XM_017321624.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGACATCC ATCAGGTGCA AGCTGCGGAC
    CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC TTCACCATTC CCCAGTCTGT CCCAGATCGC
    CTGAGGATCC GTGTGAACAA AATCAGTTTA CAAGACTATG AAGGACTCCA CTATGACAAG
    GACAAACTCC GGGAAGCTTC CATCCCTCTG GGTATTCTAG TTGTTCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGACCGT CTACAGGAGG ACTTGCAGTC AGTTTCTTCT
    GGGTCACAGA GAGTTCATTA CCAGGACCAA GAAACCTCCT TCCCCAGGGC ACTCTCAGCT
    CAGAGGCTGT CCCCACGGTG GTGCTTCCTA GATGCAACGT CTGCTGACCG CTTTTATCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCTCACGA TGAAATTTTT
    ATTCTGGACC CAGATATGGT GGTGTCACAG CAGGCAGGGA CCCCTCCAGG AATGCCTGAC
    CTGGTGGTTG AACAGGCTTC TGGGCTGTCT GACTGGTGGA ACCCTGCCCT GAGGAAACGT
    ATGCTGAGTG ACAGTGGGAT GATCACACCC CACTATGAGG ACTCAGATCT GAAAGACTTC
    AGCCACTCCA GAGTGCTACA GTCCCCAGTC TCTTCAGAAG ATCATGCAAT TTTGCAGGCA
    GTCCTCACCG GTGACCTTAT GAAGCTCATG GAGAGCTATA AGAATGGAGG GAGCTTACTA
    ATTCAGGGAC CAGGGCACTG TTCTCTCCTT CACTACGCAG CTAAGACCGG CAATGGGGAC
    ATTGTGAAGT ACATCCTTGA CCACGGACCT GCAGAATTAC TGGATATGGC AGACAGTGAA
    ACGGGCGAGA CTGCACTGCA CAAGGCTGCC TGCCAAAGGA ACCGGGCTGT ATGCCAGCTT
    CTGGTGGATG CAGGAGCATC TCTGAGACAG ACAGACTCCA AGGGCAAGAC ACCCCAGGAA
    CGAGCACAGC AGGCCGGGGA CCCAGATTTG GCTGCTTACC TAGAAAGCCG ACAGAACTAT
    AAGATCATTG GCCATGAAGA CCTGGAAACT GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177113.1
    CDS112..3450
    Translation

    Target ORF information:

    RefSeq Version XM_017321624.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGACATCC ATCAGGTGCA AGCTGCGGAC
    CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC TTCACCATTC CCCAGTCTGT CCCAGATCGC
    CTGAGGATCC GTGTGAACAA AATCAGTTTA CAAGACTATG AAGGACTCCA CTATGACAAG
    GACAAACTCC GGGAAGCTTC CATCCCTCTG GGTATTCTAG TTGTTCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGACCGT CTACAGGAGG ACTTGCAGTC AGTTTCTTCT
    GGGTCACAGA GAGTTCATTA CCAGGACCAA GAAACCTCCT TCCCCAGGGC ACTCTCAGCT
    CAGAGGCTGT CCCCACGGTG GTGCTTCCTA GATGCAACGT CTGCTGACCG CTTTTATCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCTCACGA TGAAATTTTT
    ATTCTGGACC CAGATATGGT GGTGTCACAG CAGGCAGGGA CCCCTCCAGG AATGCCTGAC
    CTGGTGGTTG AACAGGCTTC TGGGCTGTCT GACTGGTGGA ACCCTGCCCT GAGGAAACGT
    ATGCTGAGTG ACAGTGGGAT GATCACACCC CACTATGAGG ACTCAGATCT GAAAGACTTC
    AGCCACTCCA GAGTGCTACA GTCCCCAGTC TCTTCAGAAG ATCATGCAAT TTTGCAGGCA
    GTCCTCACCG GTGACCTTAT GAAGCTCATG GAGAGCTATA AGAATGGAGG GAGCTTACTA
    ATTCAGGGAC CAGGGCACTG TTCTCTCCTT CACTACGCAG CTAAGACCGG CAATGGGGAC
    ATTGTGAAGT ACATCCTTGA CCACGGACCT GCAGAATTAC TGGATATGGC AGACAGTGAA
    ACGGGCGAGA CTGCACTGCA CAAGGCTGCC TGCCAAAGGA ACCGGGCTGT ATGCCAGCTT
    CTGGTGGATG CAGGAGCATC TCTGAGACAG ACAGACTCCA AGGGCAAGAC ACCCCAGGAA
    CGAGCACAGC AGGCCGGGGA CCCAGATTTG GCTGCTTACC TAGAAAGCCG ACAGAACTAT
    AAGATCATTG GCCATGAAGA CCTGGAAACT GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81749
    Clone ID Related Accession (Same CDS sequence) XM_017321625.2 , XM_017321625.1
    Accession Version XM_017321625.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GACATCCATC AGGTGCAAGC TGCGGACCTG
    CGGCGAGTGT CTGCTCCCCC CGGCTCCTTC ACCATTCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACT TGCAGTCAGT TTCTTCTGGG
    TCACAGAGAG TTCATTACCA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTC CCCAGTCTCT TCAGAAGATC ATGCAATTTT GCAGGCAGTC
    CTCACCGGTG ACCTTATGAA GCTCATGGAG AGCTATAAGA ATGGAGGGAG CTTACTAATT
    CAGGGACCAG GGCACTGTTC TCTCCTTCAC TACGCAGCTA AGACCGGCAA TGGGGACATT
    GTGAAGTACA TCCTTGACCA CGGACCTGCA GAATTACTGG ATATGGCAGA CAGTGAAACG
    GGCGAGACTG CACTGCACAA GGCTGCCTGC CAAAGGAACC GGGCTGTATG CCAGCTTCTG
    GTGGATGCAG GAGCATCTCT GAGACAGACA GACTCCAAGG GCAAGACACC CCAGGAACGA
    GCACAGCAGG CCGGGGACCC AGATTTGGCT GCTTACCTAG AAAGCCGACA GAACTATAAG
    ATCATTGGCC ATGAAGACCT GGAAACTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177114.1
    CDS112..3447
    Translation

    Target ORF information:

    RefSeq Version XM_017321625.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321625.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GACATCCATC AGGTGCAAGC TGCGGACCTG
    CGGCGAGTGT CTGCTCCCCC CGGCTCCTTC ACCATTCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACT TGCAGTCAGT TTCTTCTGGG
    TCACAGAGAG TTCATTACCA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTC CCCAGTCTCT TCAGAAGATC ATGCAATTTT GCAGGCAGTC
    CTCACCGGTG ACCTTATGAA GCTCATGGAG AGCTATAAGA ATGGAGGGAG CTTACTAATT
    CAGGGACCAG GGCACTGTTC TCTCCTTCAC TACGCAGCTA AGACCGGCAA TGGGGACATT
    GTGAAGTACA TCCTTGACCA CGGACCTGCA GAATTACTGG ATATGGCAGA CAGTGAAACG
    GGCGAGACTG CACTGCACAA GGCTGCCTGC CAAAGGAACC GGGCTGTATG CCAGCTTCTG
    GTGGATGCAG GAGCATCTCT GAGACAGACA GACTCCAAGG GCAAGACACC CCAGGAACGA
    GCACAGCAGG CCGGGGACCC AGATTTGGCT GCTTACCTAG AAAGCCGACA GAACTATAAG
    ATCATTGGCC ATGAAGACCT GGAAACTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81750
    Clone ID Related Accession (Same CDS sequence) XM_017321626.2 , XM_017321626.1
    Accession Version XM_017321626.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGACATCC ATCAGGTGCA AGCTGCGGAC
    CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC TTCACCATTC CCCAGTCTGT CCCAGATCGC
    CTGAGGATCC GTGTGAACAA AATCAGTTTA CAAGACTATG AAGGACTCCA CTATGACAAG
    GACAAACTCC GGGAAGCTTC CATCCCTCTG GGTATTCTAG TTGTTCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGACCGT CTACAGGAGG ACCAAGAAAC CTCCTTCCCC
    AGGGCACTCT CAGCTCAGAG GCTGTCCCCA CGGTGGTGCT TCCTAGATGC AACGTCTGCT
    GACCGCTTTT ATCGAATAGA CAGATCTCAG GAACATTTGC ACTTTGTGAT GGAGATTTCT
    CACGATGAAA TTTTTATTCT GGACCCAGAT ATGGTGGTGT CACAGCAGGC AGGGACCCCT
    CCAGGAATGC CTGACCTGGT GGTTGAACAG GCTTCTGGGC TGTCTGACTG GTGGAACCCT
    GCCCTGAGGA AACGTATGCT GAGTGACAGT GGGATGATCA CACCCCACTA TGAGGACTCA
    GATCTGAAAG ACTTCAGCCA CTCCAGAGTG CTACAGTCCC CAGTCTCTTC AGAAGATCAT
    GCAATTTTGC AGGCAGTCCT CACCGGTGAC CTTATGAAGC TCATGGAGAG CTATAAGAAT
    GGAGGGAGCT TACTAATTCA GGGACCAGGG CACTGTTCTC TCCTTCACTA CGCAGCTAAG
    ACCGGCAATG GGGACATTGT GAAGTACATC CTTGACCACG GACCTGCAGA ATTACTGGAT
    ATGGCAGACA GTGAAACGGG CGAGACTGCA CTGCACAAGG CTGCCTGCCA AAGGAACCGG
    GCTGTATGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GACAGACAGA CTCCAAGGGC
    AAGACACCCC AGGAACGAGC ACAGCAGGCC GGGGACCCAG ATTTGGCTGC TTACCTAGAA
    AGCCGACAGA ACTATAAGAT CATTGGCCAT GAAGACCTGG AAACTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177115.1
    CDS112..3405
    Translation

    Target ORF information:

    RefSeq Version XM_017321626.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321626.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGACATCC ATCAGGTGCA AGCTGCGGAC
    CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC TTCACCATTC CCCAGTCTGT CCCAGATCGC
    CTGAGGATCC GTGTGAACAA AATCAGTTTA CAAGACTATG AAGGACTCCA CTATGACAAG
    GACAAACTCC GGGAAGCTTC CATCCCTCTG GGTATTCTAG TTGTTCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGACCGT CTACAGGAGG ACCAAGAAAC CTCCTTCCCC
    AGGGCACTCT CAGCTCAGAG GCTGTCCCCA CGGTGGTGCT TCCTAGATGC AACGTCTGCT
    GACCGCTTTT ATCGAATAGA CAGATCTCAG GAACATTTGC ACTTTGTGAT GGAGATTTCT
    CACGATGAAA TTTTTATTCT GGACCCAGAT ATGGTGGTGT CACAGCAGGC AGGGACCCCT
    CCAGGAATGC CTGACCTGGT GGTTGAACAG GCTTCTGGGC TGTCTGACTG GTGGAACCCT
    GCCCTGAGGA AACGTATGCT GAGTGACAGT GGGATGATCA CACCCCACTA TGAGGACTCA
    GATCTGAAAG ACTTCAGCCA CTCCAGAGTG CTACAGTCCC CAGTCTCTTC AGAAGATCAT
    GCAATTTTGC AGGCAGTCCT CACCGGTGAC CTTATGAAGC TCATGGAGAG CTATAAGAAT
    GGAGGGAGCT TACTAATTCA GGGACCAGGG CACTGTTCTC TCCTTCACTA CGCAGCTAAG
    ACCGGCAATG GGGACATTGT GAAGTACATC CTTGACCACG GACCTGCAGA ATTACTGGAT
    ATGGCAGACA GTGAAACGGG CGAGACTGCA CTGCACAAGG CTGCCTGCCA AAGGAACCGG
    GCTGTATGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GACAGACAGA CTCCAAGGGC
    AAGACACCCC AGGAACGAGC ACAGCAGGCC GGGGACCCAG ATTTGGCTGC TTACCTAGAA
    AGCCGACAGA ACTATAAGAT CATTGGCCAT GAAGACCTGG AAACTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81751
    Clone ID Related Accession (Same CDS sequence) XM_017321627.2 , XM_017321627.1
    Accession Version XM_017321627.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGATCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACT TGCAGTCAGT TTCTTCTGGG
    TCACAGAGAG TTCATTACCA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTC CCCAGTCTCT TCAGAAGATC ATGCAATTTT GCAGGCAGTC
    CTCACCGGTG ACCTTATGAA GCTCATGGAG AGCTATAAGA ATGGAGGGAG CTTACTAATT
    CAGGGACCAG GGCACTGTTC TCTCCTTCAC TACGCAGCTA AGACCGGCAA TGGGGACATT
    GTGAAGTACA TCCTTGACCA CGGACCTGCA GAATTACTGG ATATGGCAGA CAGTGAAACG
    GGCGAGACTG CACTGCACAA GGCTGCCTGC CAAAGGAACC GGGCTGTATG CCAGCTTCTG
    GTGGATGCAG GAGCATCTCT GAGACAGACA GACTCCAAGG GCAAGACACC CCAGGAACGA
    GCACAGCAGG CCGGGGACCC AGATTTGGCT GCTTACCTAG AAAGCCGACA GAACTATAAG
    ATCATTGGCC ATGAAGACCT GGAAACTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177116.1
    CDS112..3387
    Translation

    Target ORF information:

    RefSeq Version XM_017321627.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGATCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACT TGCAGTCAGT TTCTTCTGGG
    TCACAGAGAG TTCATTACCA GGACCAAGAA ACCTCCTTCC CCAGGGCACT CTCAGCTCAG
    AGGCTGTCCC CACGGTGGTG CTTCCTAGAT GCAACGTCTG CTGACCGCTT TTATCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CTCACGATGA AATTTTTATT
    CTGGACCCAG ATATGGTGGT GTCACAGCAG GCAGGGACCC CTCCAGGAAT GCCTGACCTG
    GTGGTTGAAC AGGCTTCTGG GCTGTCTGAC TGGTGGAACC CTGCCCTGAG GAAACGTATG
    CTGAGTGACA GTGGGATGAT CACACCCCAC TATGAGGACT CAGATCTGAA AGACTTCAGC
    CACTCCAGAG TGCTACAGTC CCCAGTCTCT TCAGAAGATC ATGCAATTTT GCAGGCAGTC
    CTCACCGGTG ACCTTATGAA GCTCATGGAG AGCTATAAGA ATGGAGGGAG CTTACTAATT
    CAGGGACCAG GGCACTGTTC TCTCCTTCAC TACGCAGCTA AGACCGGCAA TGGGGACATT
    GTGAAGTACA TCCTTGACCA CGGACCTGCA GAATTACTGG ATATGGCAGA CAGTGAAACG
    GGCGAGACTG CACTGCACAA GGCTGCCTGC CAAAGGAACC GGGCTGTATG CCAGCTTCTG
    GTGGATGCAG GAGCATCTCT GAGACAGACA GACTCCAAGG GCAAGACACC CCAGGAACGA
    GCACAGCAGG CCGGGGACCC AGATTTGGCT GCTTACCTAG AAAGCCGACA GAACTATAAG
    ATCATTGGCC ATGAAGACCT GGAAACTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81752
    Clone ID Related Accession (Same CDS sequence) XM_017321628.1 , XM_017321628.2
    Accession Version XM_017321628.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GATCCCCAGT CTGTCCCAGA TCGCCTGAGG
    ATCCGTGTGA ACAAAATCAG TTTACAAGAC TATGAAGGAC TCCACTATGA CAAGGACAAA
    CTCCGGGAAG CTTCCATCCC TCTGGGTATT CTAGTTGTTC GTGGAGACTG TGATTTGGAG
    ACTTGCCGTA TGTACATAGA CCGTCTACAG GAGGACTTGC AGTCAGTTTC TTCTGGGTCA
    CAGAGAGTTC ATTACCAGGA CCAAGAAACC TCCTTCCCCA GGGCACTCTC AGCTCAGAGG
    CTGTCCCCAC GGTGGTGCTT CCTAGATGCA ACGTCTGCTG ACCGCTTTTA TCGAATAGAC
    AGATCTCAGG AACATTTGCA CTTTGTGATG GAGATTTCTC ACGATGAAAT TTTTATTCTG
    GACCCAGATA TGGTGGTGTC ACAGCAGGCA GGGACCCCTC CAGGAATGCC TGACCTGGTG
    GTTGAACAGG CTTCTGGGCT GTCTGACTGG TGGAACCCTG CCCTGAGGAA ACGTATGCTG
    AGTGACAGTG GGATGATCAC ACCCCACTAT GAGGACTCAG ATCTGAAAGA CTTCAGCCAC
    TCCAGAGTGC TACAGTCCCC AGTCTCTTCA GAAGATCATG CAATTTTGCA GGCAGTCCTC
    ACCGGTGACC TTATGAAGCT CATGGAGAGC TATAAGAATG GAGGGAGCTT ACTAATTCAG
    GGACCAGGGC ACTGTTCTCT CCTTCACTAC GCAGCTAAGA CCGGCAATGG GGACATTGTG
    AAGTACATCC TTGACCACGG ACCTGCAGAA TTACTGGATA TGGCAGACAG TGAAACGGGC
    GAGACTGCAC TGCACAAGGC TGCCTGCCAA AGGAACCGGG CTGTATGCCA GCTTCTGGTG
    GATGCAGGAG CATCTCTGAG ACAGACAGAC TCCAAGGGCA AGACACCCCA GGAACGAGCA
    CAGCAGGCCG GGGACCCAGA TTTGGCTGCT TACCTAGAAA GCCGACAGAA CTATAAGATC
    ATTGGCCATG AAGACCTGGA AACTGCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177117.1
    CDS112..3384
    Translation

    Target ORF information:

    RefSeq Version XM_017321628.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGGAAA CCAAAGGCCG TCCTTTTGTG
    ATCAAGCCCA TCTCCTCCCC TCTCATGAAG CCTTTGCTTG TCTTTGTGAA CCCAAAGAGT
    GGGGGAAACC AGGGAACCAA AGTCCTACAG ATGTTTATGT GGTACCTGAA TCCACGGCAA
    GTCTTTGATC TTTCTCAGGA AGGGCCAAAA GATGCGCTGG AGATGTACAG GAAGGTACCA
    AATCTGAGAA TTCTGGCCTG CGGTGGGGAT GGAACGGTGG GCTGGATCCT CTCCATCCTG
    GACGAGCTGC AGCTGAGCCC ACAGCCTCCT GTGGGGGTGC TTCCACTGGG GACTGGGAAT
    GACCTGGCTC GGACGCTCAA CTGGGGAGGG GGTTACACCG ATGAGCCTGT TTCTAAGATC
    CTGTGCCAGG TGGAAGATGG GACCATTGTA CAGTTGGATC GCTGGAACCT CCACGTAGAG
    AGAAACCCAG ACCTGCCGCC CGAAGAACTT GAAGATGGCG TGTGTAAGCT TCCTCTGAAT
    GTCTTCAATA ATTACTTCAG CCTTGGATTT GATGCTCACG TCACATTGGA GTTCCATGAA
    TCCCGAGAAG CAAATCCAGA GAAATTCAAT AGTCGTTTTC GAAATAAAAT GTTCTATGCA
    GGGGCAGCCT TTTCTGATTT CCTACAAAGA AGTTCTAGAG ATCTATCCAA ACACGTCAAA
    GTTGTCTGTG ATGGGACAGA CCTCACCCCA AAGATCCAGG ACCTGAAGTT CCAGTGTATA
    GTATTTTTAA ATATTCCCAG ATATTGTGCT GGCACAATGC CTTGGGGAAA CCCTGGAGAT
    CATCATGACT TTGAACCCCA ACGTCATGAT GACGGCTACA TTGAAGTCAT TGGATTCACT
    ATGGCCTCTT TGGCTGCCCT GCAAGTTGGG GGGCACGGAG AAAGGCTGCA CCAGTGTCGG
    GAAGTGATGC TTCTGACTTA CAAGTCCATT CCCATGCAAG TGGACGGGGA GCCATGTCGG
    TTGGCACCTG CTATGATTCG GATCTCCTTG AGGAATCAGG CCAACATGGT ACAGAAGAGC
    AAGCGGAGAA CCTCGATGCC TTTGCTCAAT GATCCCCAGT CTGTCCCAGA TCGCCTGAGG
    ATCCGTGTGA ACAAAATCAG TTTACAAGAC TATGAAGGAC TCCACTATGA CAAGGACAAA
    CTCCGGGAAG CTTCCATCCC TCTGGGTATT CTAGTTGTTC GTGGAGACTG TGATTTGGAG
    ACTTGCCGTA TGTACATAGA CCGTCTACAG GAGGACTTGC AGTCAGTTTC TTCTGGGTCA
    CAGAGAGTTC ATTACCAGGA CCAAGAAACC TCCTTCCCCA GGGCACTCTC AGCTCAGAGG
    CTGTCCCCAC GGTGGTGCTT CCTAGATGCA ACGTCTGCTG ACCGCTTTTA TCGAATAGAC
    AGATCTCAGG AACATTTGCA CTTTGTGATG GAGATTTCTC ACGATGAAAT TTTTATTCTG
    GACCCAGATA TGGTGGTGTC ACAGCAGGCA GGGACCCCTC CAGGAATGCC TGACCTGGTG
    GTTGAACAGG CTTCTGGGCT GTCTGACTGG TGGAACCCTG CCCTGAGGAA ACGTATGCTG
    AGTGACAGTG GGATGATCAC ACCCCACTAT GAGGACTCAG ATCTGAAAGA CTTCAGCCAC
    TCCAGAGTGC TACAGTCCCC AGTCTCTTCA GAAGATCATG CAATTTTGCA GGCAGTCCTC
    ACCGGTGACC TTATGAAGCT CATGGAGAGC TATAAGAATG GAGGGAGCTT ACTAATTCAG
    GGACCAGGGC ACTGTTCTCT CCTTCACTAC GCAGCTAAGA CCGGCAATGG GGACATTGTG
    AAGTACATCC TTGACCACGG ACCTGCAGAA TTACTGGATA TGGCAGACAG TGAAACGGGC
    GAGACTGCAC TGCACAAGGC TGCCTGCCAA AGGAACCGGG CTGTATGCCA GCTTCTGGTG
    GATGCAGGAG CATCTCTGAG ACAGACAGAC TCCAAGGGCA AGACACCCCA GGAACGAGCA
    CAGCAGGCCG GGGACCCAGA TTTGGCTGCT TACCTAGAAA GCCGACAGAA CTATAAGATC
    ATTGGCCATG AAGACCTGGA AACTGCTGTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81753
    Clone ID Related Accession (Same CDS sequence) XM_017321629.2 , XM_017321629.1
    Accession Version XM_017321629.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGATCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACC AAGAAACCTC CTTCCCCAGG
    GCACTCTCAG CTCAGAGGCT GTCCCCACGG TGGTGCTTCC TAGATGCAAC GTCTGCTGAC
    CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCTCAC
    GATGAAATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCAGGCAGG GACCCCTCCA
    GGAATGCCTG ACCTGGTGGT TGAACAGGCT TCTGGGCTGT CTGACTGGTG GAACCCTGCC
    CTGAGGAAAC GTATGCTGAG TGACAGTGGG ATGATCACAC CCCACTATGA GGACTCAGAT
    CTGAAAGACT TCAGCCACTC CAGAGTGCTA CAGTCCCCAG TCTCTTCAGA AGATCATGCA
    ATTTTGCAGG CAGTCCTCAC CGGTGACCTT ATGAAGCTCA TGGAGAGCTA TAAGAATGGA
    GGGAGCTTAC TAATTCAGGG ACCAGGGCAC TGTTCTCTCC TTCACTACGC AGCTAAGACC
    GGCAATGGGG ACATTGTGAA GTACATCCTT GACCACGGAC CTGCAGAATT ACTGGATATG
    GCAGACAGTG AAACGGGCGA GACTGCACTG CACAAGGCTG CCTGCCAAAG GAACCGGGCT
    GTATGCCAGC TTCTGGTGGA TGCAGGAGCA TCTCTGAGAC AGACAGACTC CAAGGGCAAG
    ACACCCCAGG AACGAGCACA GCAGGCCGGG GACCCAGATT TGGCTGCTTA CCTAGAAAGC
    CGACAGAACT ATAAGATCAT TGGCCATGAA GACCTGGAAA CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177118.1
    CDS112..3342
    Translation

    Target ORF information:

    RefSeq Version XM_017321629.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGATCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACC AAGAAACCTC CTTCCCCAGG
    GCACTCTCAG CTCAGAGGCT GTCCCCACGG TGGTGCTTCC TAGATGCAAC GTCTGCTGAC
    CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCTCAC
    GATGAAATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCAGGCAGG GACCCCTCCA
    GGAATGCCTG ACCTGGTGGT TGAACAGGCT TCTGGGCTGT CTGACTGGTG GAACCCTGCC
    CTGAGGAAAC GTATGCTGAG TGACAGTGGG ATGATCACAC CCCACTATGA GGACTCAGAT
    CTGAAAGACT TCAGCCACTC CAGAGTGCTA CAGTCCCCAG TCTCTTCAGA AGATCATGCA
    ATTTTGCAGG CAGTCCTCAC CGGTGACCTT ATGAAGCTCA TGGAGAGCTA TAAGAATGGA
    GGGAGCTTAC TAATTCAGGG ACCAGGGCAC TGTTCTCTCC TTCACTACGC AGCTAAGACC
    GGCAATGGGG ACATTGTGAA GTACATCCTT GACCACGGAC CTGCAGAATT ACTGGATATG
    GCAGACAGTG AAACGGGCGA GACTGCACTG CACAAGGCTG CCTGCCAAAG GAACCGGGCT
    GTATGCCAGC TTCTGGTGGA TGCAGGAGCA TCTCTGAGAC AGACAGACTC CAAGGGCAAG
    ACACCCCAGG AACGAGCACA GCAGGCCGGG GACCCAGATT TGGCTGCTTA CCTAGAAAGC
    CGACAGAACT ATAAGATCAT TGGCCATGAA GACCTGGAAA CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81754
    Clone ID Related Accession (Same CDS sequence) XM_017321630.1 , XM_017321630.2
    Accession Version XM_017321630.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGACATCC ATCAGGTGCA AGCTGCGGAC
    CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC TTCACCATTC CCCAGTCTGT CCCAGATCGC
    CTGAGGATCC GTGTGAACAA AATCAGTTTA CAAGACTATG AAGGACTCCA CTATGACAAG
    GACAAACTCC GGGAAGCTTC CATCCCTCTG GGTATTCTAG TTGTTCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGACCGT CTACAGGAGG ACTTGCAGTC AGTTTCTTCT
    GGGTCACAGA GAGTTCATTA CCAGGACCAA GAAACCTCCT TCCCCAGGGC ACTCTCAGCT
    CAGAGGCTGT CCCCACGGTG GTGCTTCCTA GATGCAACGT CTGCTGACCG CTTTTATCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCTCACGA TGAAATTTTT
    ATTCTGGACC CAGATATGGT GGTGTCACAG CAGGCAGGGA CCCCTCCAGG AATGCCTGAC
    CTGGTGGTTG AACAGGCTTC TGGGCTGTCT GACTGGTGGA ACCCTGCCCT GAGGAAACGT
    ATGCTGAGTG ACAGTGGGAT GATCACACCC CACTATGAGG ACTCAGATCT GAAAGACTTC
    AGCCACTCCA GAGTGCTACA GTGTGATCAT GGTTGGAAAG CAACATGCTG CTTGGAATTT
    TGCAGAAGAA CTGATACTTT GGGCTATGCG AAATCATCTT ATCTGCCCAT ATTTTGCTAC
    AGGAACACGT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177119.1
    CDS112..3126
    Translation

    Target ORF information:

    RefSeq Version XM_017321630.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGACATCC ATCAGGTGCA AGCTGCGGAC
    CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC TTCACCATTC CCCAGTCTGT CCCAGATCGC
    CTGAGGATCC GTGTGAACAA AATCAGTTTA CAAGACTATG AAGGACTCCA CTATGACAAG
    GACAAACTCC GGGAAGCTTC CATCCCTCTG GGTATTCTAG TTGTTCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGACCGT CTACAGGAGG ACTTGCAGTC AGTTTCTTCT
    GGGTCACAGA GAGTTCATTA CCAGGACCAA GAAACCTCCT TCCCCAGGGC ACTCTCAGCT
    CAGAGGCTGT CCCCACGGTG GTGCTTCCTA GATGCAACGT CTGCTGACCG CTTTTATCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCTCACGA TGAAATTTTT
    ATTCTGGACC CAGATATGGT GGTGTCACAG CAGGCAGGGA CCCCTCCAGG AATGCCTGAC
    CTGGTGGTTG AACAGGCTTC TGGGCTGTCT GACTGGTGGA ACCCTGCCCT GAGGAAACGT
    ATGCTGAGTG ACAGTGGGAT GATCACACCC CACTATGAGG ACTCAGATCT GAAAGACTTC
    AGCCACTCCA GAGTGCTACA GTGTGATCAT GGTTGGAAAG CAACATGCTG CTTGGAATTT
    TGCAGAAGAA CTGATACTTT GGGCTATGCG AAATCATCTT ATCTGCCCAT ATTTTGCTAC
    AGGAACACGT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81756
    Clone ID Related Accession (Same CDS sequence) XM_017321632.1 , XM_017321632.2
    Accession Version XM_017321632.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGATCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACC AAGAAACCTC CTTCCCCAGG
    GCACTCTCAG CTCAGAGGCT GTCCCCACGG TGGTGCTTCC TAGATGCAAC GTCTGCTGAC
    CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCTCAC
    GATGAAATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCAGGCAGG GACCCCTCCA
    GGAATGCCTG ACCTGGTGGT TGAACAGGCT TCTGGGCTGT CTGACTGGTG GAACCCTGCC
    CTGAGGAAAC GTATGCTGAG TGACAGTGGG ATGATCACAC CCCACTATGA GGACTCAGAT
    CTGAAAGACT TCAGCCACTC CAGAGTGCTA CAGTGTGATC ATGGTTGGAA AGCAACATGC
    TGCTTGGAAT TTTGCAGAAG AACTGATACT TTGGGCTATG CGAAATCATC TTATCTGCCC
    ATATTTTGCT ACAGGAACAC GTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177121.1
    CDS112..3018
    Translation

    Target ORF information:

    RefSeq Version XM_017321632.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321632.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT GTGATGGGAC AGACCTCACC CCAAAGATCC AGGACCTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATTCC CAGATATTGT GCTGGCACAA TGCCTTGGGG AAACCCTGGA
    GATCATCATG ACTTTGAACC CCAACGTCAT GATGACGGCT ACATTGAAGT CATTGGATTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGGCACG GAGAAAGGCT GCACCAGTGT
    CGGGAAGTGA TGCTTCTGAC TTACAAGTCC ATTCCCATGC AAGTGGACGG GGAGCCATGT
    CGGTTGGCAC CTGCTATGAT TCGGATCTCC TTGAGGAATC AGGCCAACAT GGTACAGAAG
    AGCAAGCGGA GAACCTCGAT GCCTTTGCTC AATGATCCCC AGTCTGTCCC AGATCGCCTG
    AGGATCCGTG TGAACAAAAT CAGTTTACAA GACTATGAAG GACTCCACTA TGACAAGGAC
    AAACTCCGGG AAGCTTCCAT CCCTCTGGGT ATTCTAGTTG TTCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGACCGTCTA CAGGAGGACC AAGAAACCTC CTTCCCCAGG
    GCACTCTCAG CTCAGAGGCT GTCCCCACGG TGGTGCTTCC TAGATGCAAC GTCTGCTGAC
    CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCTCAC
    GATGAAATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCAGGCAGG GACCCCTCCA
    GGAATGCCTG ACCTGGTGGT TGAACAGGCT TCTGGGCTGT CTGACTGGTG GAACCCTGCC
    CTGAGGAAAC GTATGCTGAG TGACAGTGGG ATGATCACAC CCCACTATGA GGACTCAGAT
    CTGAAAGACT TCAGCCACTC CAGAGTGCTA CAGTGTGATC ATGGTTGGAA AGCAACATGC
    TGCTTGGAAT TTTGCAGAAG AACTGATACT TTGGGCTATG CGAAATCATC TTATCTGCCC
    ATATTTTGCT ACAGGAACAC GTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81757
    Clone ID Related Accession (Same CDS sequence) XM_017321633.2 , XM_017321633.1
    Accession Version XM_017321633.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product diacylglycerol kinase iota isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT TATCTTGGAT GAGACTCTCA GACTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177122.1
    CDS112..2010
    Translation

    Target ORF information:

    RefSeq Version XM_017321633.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
    CGCGCTCCCG CCGCCTCCAG CGCCCTCAGC CCGACCGGCC TCTGCAGCGG CACCACCTCG
    GCTTCTTTCG CGGCCGCCGG AGCGGTCGCC ATGAACCCCA GCTCCTCGGC GGGAGAGGAG
    AGAGGGGCGA CGGGTGGCAG CAGCAGCAGC GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC
    GCCGAGGGCG GCGCGGACCC GCGGGGTGCA GGGGCAGCGG CTGCTGCTGC CTTGGAGGAG
    CCCGCGGCCG CCGGACAGAA AGAGAAGGAA GAGGCGCTGG AGGAGAAGCT GAGGGACTTA
    ACTTTCCGGA AGCAGGTCTC TTACAGGAAA GCCATCTCCC GGACAGGCCT CCAGCATCTG
    GCACCTGCAC ATCCTCTTGG TCTTCCTGTG GCTAACGGTC CAGCGAAGGA GCCCAGAGCC
    ACTCTGGACT GGAGCGAGAA TGCTGTGAAT GGGGAGCACC TGTGGCTGGA GACCAACGTT
    TCAGGGGACC TCTGCTACCT GGGGGAGGAG AACTGCCAAG TCCGATTTGC AAAATCCGCT
    CTCAGGAGGA AGTGTGCCGT CTGCAAAATC GTGGTCCACA CAGCCTGCAT TGAGCAACTA
    GAGAAGATTA ATTTCAGATG TAAACCAACA TTTCGGGAAG GAGGCTCCAG ATCGCCAAGA
    GAAAACTTTG TGCGTCACCA CTGGGTCCAC AGGAGGCGGC AGGAGGGAAA ATGTAAGCAG
    TGTGGTAAGG GCTTCCAGCA AAAGTTCTCC TTCCACAGTA AAGAGATCGT GGCCATCAGC
    TGTTCTTGGT GCAAGCAGGC GTTTCACAAT AAAGTGACCT GCTTCATGCT GCATCATATT
    GAGGAACCCT GCTCCCTGGG GGCTCACGCC GCTGTTATTG TCCCGCCCAC CTGGATCATT
    AAGGTGAAGA AACCTCAGGT GATCTCCATA CCAGGAGATG AACTTGAACT TGGTTGGGCG
    GGAGACGGAC CTGGTTGGGC GGGAGACTGG AGCCCCTCAG AGAATTTGGG ATTCTTCCAT
    TTTTACTGGG AGGGTTGGCA GAACTCCCTG AAGGCTTCCA ACCGGAAGAA GAAGAGGACA
    AGTTTTAAAA GGAAAGCCAG TAAAAGAGGA ACTGAGCAGG AAACCAAAGG CCGTCCTTTT
    GTGATCAAGC CCATCTCCTC CCCTCTCATG AAGCCTTTGC TTGTCTTTGT GAACCCAAAG
    AGTGGGGGAA ACCAGGGAAC CAAAGTCCTA CAGATGTTTA TGTGGTACCT GAATCCACGG
    CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TGGAGATGTA CAGGAAGGTA
    CCAAATCTGA GAATTCTGGC CTGCGGTGGG GATGGAACGG TGGGCTGGAT CCTCTCCATC
    CTGGACGAGC TGCAGCTGAG CCCACAGCCT CCTGTGGGGG TGCTTCCACT GGGGACTGGG
    AATGACCTGG CTCGGACGCT CAACTGGGGA GGGGGTTACA CCGATGAGCC TGTTTCTAAG
    ATCCTGTGCC AGGTGGAAGA TGGGACCATT GTACAGTTGG ATCGCTGGAA CCTCCACGTA
    GAGAGAAACC CAGACCTGCC GCCCGAAGAA CTTGAAGATG GCGTGTGTAA GCTTCCTCTG
    AATGTCTTCA ATAATTACTT CAGCCTTGGA TTTGATGCTC ACGTCACATT GGAGTTCCAT
    GAATCCCGAG AAGCAAATCC AGAGAAATTC AATAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CCTTTTCTGA TTTCCTACAA AGAAGTTCTA GAGATCTATC CAAACACGTC
    AAAGTTGTCT TATCTTGGAT GAGACTCTCA GACTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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