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EBI3 cDNA ORF clone, Mus musculus(House mouse)

Gene Symbol EBI3
Entrez Gene ID 50498
Full Name Epstein-Barr virus induced gene 3
Synonyms EBI-3,IL-27
General protein information
Preferred Names

Epstein-Barr virus induced gene 3

Names

interleukin-27 subunit beta
IL-27 subunit beta
IL-27B
epstein-Barr virus-induced gene 3 protein homolog

Gene Type protein-coding
Organism Mus musculus(House mouse)
Genome

17

17 D|17 29.08 cM


mRNA and Protein(s)

mRNA Protein Name
NM_015766.2 NP_056581.1 interleukin-27 subunit beta precursor
XM_006524599.3 XP_006524662.1 interleukin-27 subunit beta isoform X1



Homo sapiens (human) EBI3 NP_005746.2
Danio rerio (zebrafish) ebi3 NP_001106980.1
Pan troglodytes (chimpanzee) EBI3 XP_001138182.1
Canis lupus familiaris (dog) EBI3 XP_542161.3
Xenopus tropicalis (tropical clawed frog) ebi3 XP_002934057.1
Bos taurus (cattle) EBI3 NP_001093835.1
Mus musculus (house mouse) Ebi3 NP_056581.1
Rattus norvegicus (Norway rat) Ebi3 NP_001102891.1


Related articles in PubMed

DR3 signaling modulates the function of Foxp3+ regulatory T cells and the severity of acute graft-versus-host disease.
Nishikii H, Kim BS, Yokoyama Y, Chen Y, Baker J, Pierini A, Alvarez M, Mavers M, Maas-Bauer K, Pan Y, Chiba S, Negrin RS
Blood128(24)2846-2858(2016 Dec)

Interleukin (IL)-39 [IL-23p19/Epstein-Barr virus-induced 3 (Ebi3)] induces differentiation/expansion of neutrophils in lupus-prone mice.
Wang X, Liu X, Zhang Y, Wang Z, Zhu G, Han G, Chen G, Hou C, Wang T, Ma N, Shen B, Li Y, Xiao H, Wang R
Clinical and experimental immunology186(2)144-156(2016 Nov)

Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines.
Hou C, Wu Q, Ouyang C, Huang T
International journal of molecular medicine38(3)713-20(2016 Sep)

Interleukin-35 attenuates collagen-induced arthritis through suppression of vascular endothelial growth factor and its receptors.
Wu S, Li Y, Li Y, Yao L, Lin T, Jiang S, Shen H, Xia L, Lu J
International immunopharmacology3471-7(2016 May)

Interleukin-35 is upregulated in response to influenza virus infection and secondary bacterial pneumonia.
Chen Y, Wang CJ, Lin SH, Zhang M, Li SY, Xu F
Cytokine8123-7(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EBI3 can mediate IL-6 trans-signaling in a process that involves gp130
Title: Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6

our results suggest that IL-39 induces differentiation and/or expansion of neutrophils in lupus-prone mice.
Title: Interleukin (IL)-39 [IL-23p19/Epstein-Barr virus-induced 3 (Ebi3)] induces differentiation/expansion of neutrophils in lupus-prone mice.

Data show that interleukin-35 (Epstein-Barr virus-induced gene 3 (EBI3) and the interleukin-12 Subunit p35 (p35) subunit) expressing plasmid downregulates the expression of pro-inflammatory cytokines and upregulates the expression of anti-inflammatory cytokines.
Title: Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines.

we describe here a new IL-12 member IL-39 (IL-23p19/Ebi3) secreted by activated B cells that mediate inflammatory responses in lupus-like mice. Thus, IL-39 might contribute to immunopathogenic mechanisms of SLE.
Title: A novel IL-23p19/Ebi3 (IL-39) cytokine mediates inflammation in Lupus-like mice.

IL-35 treatment reduced collagen-induced arthritis via inhibiting vascular endothelial growth factor and its receptors
Title: Interleukin-35 attenuates collagen-induced arthritis through suppression of vascular endothelial growth factor and its receptors.

The following EBI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EBI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OMu22389 NM_015766.2
Latest version!
Mus musculus Epstein-Barr virus induced gene 3 (Ebi3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OMu53816 XM_006524599.3
Latest version!
Mus musculus Epstein-Barr virus induced gene 3 (Ebi3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu22389
Clone ID Related Accession (Same CDS sequence) NM_015766.2
Accession Version NM_015766.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 687bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Mus musculus(House mouse)
Product interleukin-27 subunit beta precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY134465.1, CX240802.1, BI134394.1 and AW495532.1. On Jul 15, 2005 this sequence version replaced NM_015766.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008209.1, AF013114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGTCCAAGC TGCTCTTCCT GTCACTTGCC CTCTGGGCCA GCCGCTCCCC TGGTTACACT 
GAAACAGCTC TCGTGGCTCT AAGCCAGCCC AGAGTGCAAT GCCATGCTTC TCGGTATCCC
GTGGCCGTGG ACTGCTCCTG GACTCCTCTC CAGGCTCCCA ACTCCACCAG ATCCACGTCC
TTCATTGCCA CTTACAGGCT CGGTGTGGCC ACCCAGCAGC AGAGCCAGCC CTGCCTACAA
CGGAGCCCCC AGGCCTCCCG ATGCACCATC CCCGACGTGC ACCTGTTCTC CACGGTGCCC
TACATGCTAA ATGTCACTGC AGTGCACCCA GGCGGCGCCA GCAGCAGCCT CCTAGCCTTT
GTGGCTGAGC GAATCATCAA GCCGGACCCT CCGGAAGGCG TGCGCCTGCG CACAGCGGGA
CAGCGCCTGC AGGTGCTCTG GCATCCCCCT GCTTCCTGGC CCTTCCCGGA CATCTTCTCT
CTCAAGTACC GACTCCGCTA CCGGCGCCGA GGAGCCTCTC ACTTCCGCCA GGTGGGACCC
ATTGAAGCCA CGACTTTCAC CCTCAGGAAC TCGAAACCCC ATGCCAAGTA TTGCATCCAG
GTGTCAGCTC AGGACCTCAC AGATTATGGG AAACCAAGTG ACTGGAGCCT CCCTGGGCAA
GTAGAAAGTG CACCCCATAA GCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_056581.1
CDS240..926
Misc Feature(1)621..887
Misc Feature(2)order(621..623,816..818,861..863)
Misc Feature(3)order(864..869,873..878)
Exon (1)1..306
Exon (2)307..436
Exon (3)437..615
Exon (4)616..770
Exon (5)771..1171
Translation

Target ORF information:

RefSeq Version NM_015766.2
Organism Mus musculus(House mouse)
Definition Mus musculus Epstein-Barr virus induced gene 3 (Ebi3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_015766.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGTCCAAGC TGCTCTTCCT GTCACTTGCC CTCTGGGCCA GCCGCTCCCC TGGTTACACT 
GAAACAGCTC TCGTGGCTCT AAGCCAGCCC AGAGTGCAAT GCCATGCTTC TCGGTATCCC
GTGGCCGTGG ACTGCTCCTG GACTCCTCTC CAGGCTCCCA ACTCCACCAG ATCCACGTCC
TTCATTGCCA CTTACAGGCT CGGTGTGGCC ACCCAGCAGC AGAGCCAGCC CTGCCTACAA
CGGAGCCCCC AGGCCTCCCG ATGCACCATC CCCGACGTGC ACCTGTTCTC CACGGTGCCC
TACATGCTAA ATGTCACTGC AGTGCACCCA GGCGGCGCCA GCAGCAGCCT CCTAGCCTTT
GTGGCTGAGC GAATCATCAA GCCGGACCCT CCGGAAGGCG TGCGCCTGCG CACAGCGGGA
CAGCGCCTGC AGGTGCTCTG GCATCCCCCT GCTTCCTGGC CCTTCCCGGA CATCTTCTCT
CTCAAGTACC GACTCCGCTA CCGGCGCCGA GGAGCCTCTC ACTTCCGCCA GGTGGGACCC
ATTGAAGCCA CGACTTTCAC CCTCAGGAAC TCGAAACCCC ATGCCAAGTA TTGCATCCAG
GTGTCAGCTC AGGACCTCAC AGATTATGGG AAACCAAGTG ACTGGAGCCT CCCTGGGCAA
GTAGAAAGTG CACCCCATAA GCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu53816
Clone ID Related Accession (Same CDS sequence) XM_006524599.3
Accession Version XM_006524599.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 384bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product interleukin-27 subunit beta isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524599.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
ATGCTAAATG TCACTGCAGT GCACCCAGGC GGCGCCAGCA GCAGCCTCCT AGCCTTTGTG 
GCTGAGCGAA TCATCAAGCC GGACCCTCCG GAAGGCGTGC GCCTGCGCAC AGCGGGACAG
CGCCTGCAGG TGCTCTGGCA TCCCCCTGCT TCCTGGCCCT TCCCGGACAT CTTCTCTCTC
AAGTACCGAC TCCGCTACCG GCGCCGAGGA GCCTCTCACT TCCGCCAGGT GGGACCCATT
GAAGCCACGA CTTTCACCCT CAGGAACTCG AAACCCCATG CCAAGTATTG CATCCAGGTG
TCAGCTCAGG ACCTCACAGA TTATGGGAAA CCAAGTGACT GGAGCCTCCC TGGGCAAGTA
GAAAGTGCAC CCCATAAGCC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006524662.1
CDS195..578
Misc Feature(1)273..539
Misc Feature(2)order(273..275,468..470,513..515)
Misc Feature(3)order(516..521,525..530)
Translation

Target ORF information:

RefSeq Version XM_006524599.3
Organism Mus musculus(House mouse)
Definition Mus musculus Epstein-Barr virus induced gene 3 (Ebi3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006524599.3

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGCTAAATG TCACTGCAGT GCACCCAGGC GGCGCCAGCA GCAGCCTCCT AGCCTTTGTG 
GCTGAGCGAA TCATCAAGCC GGACCCTCCG GAAGGCGTGC GCCTGCGCAC AGCGGGACAG
CGCCTGCAGG TGCTCTGGCA TCCCCCTGCT TCCTGGCCCT TCCCGGACAT CTTCTCTCTC
AAGTACCGAC TCCGCTACCG GCGCCGAGGA GCCTCTCACT TCCGCCAGGT GGGACCCATT
GAAGCCACGA CTTTCACCCT CAGGAACTCG AAACCCCATG CCAAGTATTG CATCCAGGTG
TCAGCTCAGG ACCTCACAGA TTATGGGAAA CCAAGTGACT GGAGCCTCCC TGGGCAAGTA
GAAAGTGCAC CCCATAAGCC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

DR3 signaling modulates the function of Foxp3+ regulatory T cells and the severity of acute graft-versus-host disease.
Blood128(24)2846-2858(2016 Dec)
Nishikii H,Kim BS,Yokoyama Y,Chen Y,Baker J,Pierini A,Alvarez M,Mavers M,Maas-Bauer K,Pan Y,Chiba S,Negrin RS


book

Interleukin (IL)-39 [IL-23p19/Epstein-Barr virus-induced 3 (Ebi3)] induces differentiation/expansion of neutrophils in lupus-prone mice.
Clinical and experimental immunology186(2)144-156(2016 Nov)
Wang X,Liu X,Zhang Y,Wang Z,Zhu G,Han G,Chen G,Hou C,Wang T,Ma N,Shen B,Li Y,Xiao H,Wang R


book

Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines.
International journal of molecular medicine38(3)713-20(2016 Sep)
Hou C,Wu Q,Ouyang C,Huang T


book

Interleukin-35 attenuates collagen-induced arthritis through suppression of vascular endothelial growth factor and its receptors.
International immunopharmacology3471-7(2016 May)
Wu S,Li Y,Li Y,Yao L,Lin T,Jiang S,Shen H,Xia L,Lu J


book

Interleukin-35 is upregulated in response to influenza virus infection and secondary bacterial pneumonia.
Cytokine8123-7(2016 May)
Chen Y,Wang CJ,Lin SH,Zhang M,Li SY,Xu F


 
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