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Ppp4c cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Ppp4c
Entrez Gene ID 56420
Full Name protein phosphatase 4, catalytic subunit
Synonyms 1110002D08Rik,AU016079,Ppx
General protein information
Preferred Names

protein phosphatase 4, catalytic subunit

Names

serine/threonine-protein phosphatase 4 catalytic subunit
PP-X
PP4C
pp4
protein phosphatase X
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

7

7|7 F3

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001360464.1 NP_001347393.1 serine/threonine-protein phosphatase 4 catalytic subunit
NM_019674.3 NP_062648.1 serine/threonine-protein phosphatase 4 catalytic subunit
XM_006508049.1 XP_006508112.1 serine/threonine-protein phosphatase 4 catalytic subunit isoform X1
XM_006508049.2 XP_006508112.1 serine/threonine-protein phosphatase 4 catalytic subunit isoform X1
NM_019674.4 NP_062648.1 serine/threonine-protein phosphatase 4 catalytic subunit

Pathways from BioSystems


Homologies


PPP4C XP_001108500.1
Canis lupus familiaris (dog) PPP4C XP_547067.1
Rattus norvegicus (Norway rat) Ppp4c NP_599186.1
Danio rerio (zebrafish) ppp4cb NP_001104638.1
Kluyveromyces lactis KLLA0E10055g XP_454403.1
Drosophila melanogaster (fruit fly) Pp4-19C NP_524803.1
Neurospora crassa NCU08301 XP_962514.2
Arabidopsis thaliana (thale cress) PPX1 NP_194402.1
Oryza sativa (rice) Os09g0284200 NP_001062774.1
Pan troglodytes (chimpanzee) PPP4C XP_001148577.1
Eremothecium gossypii AGOS_AGR136W NP_986802.2
Schizosaccharomyces pombe (fission yeast) SPBC26H8.05c NP_596646.1
Xenopus tropicalis (tropical clawed frog) ppp4c NP_988943.1
Bos taurus (cattle) PPP4C NP_001092578.1
Mus musculus (house mouse) Ppp4c NP_062648.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP003779 XP_310323.5
Caenorhabditis elegans (roundworm) pph-4.1 NP_499603.1
Homo sapiens (human) PPP4C NP_002711.1
Saccharomyces cerevisiae (baker's yeast) PPH3 NP_010360.1
Magnaporthe oryzae (rice blast fungus) MGG_01528 XP_003714501.1
Arabidopsis thaliana (thale cress) PPX2 NP_200337.1

Gene Ontology


Bibliography

Related articles in PubMed

Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.
Hwang J, Lee JA, Pallas DC
The Journal of biological chemistry291(40)21008-21019(2016 Sep)

T cell proliferation and adaptive immune responses are critically regulated by protein phosphatase 4.
Liao FH, Hsiao WY, Lin YC, Chan YC, Huang CY
Cell cycle (Georgetown, Tex.)15(8)1073-83(2016)

Protein phosphatase 4 (PP4) functions as a critical regulator in tumor necrosis factor (TNF)-?-induced hepatic insulin resistance.
Zhao H, Huang X, Jiao J, Zhang H, Liu J, Qin W, Meng X, Shen T, Lin Y, Chu J, Li J
Scientific reports518093(2015 Dec)

Phosphatase PP4 Negatively Regulates Type I IFN Production and Antiviral Innate Immunity by Dephosphorylating and Deactivating TBK1.
Zhan Z, Cao H, Xie X, Yang L, Zhang P, Chen Y, Fan H, Liu Z, Liu X
Journal of immunology (Baltimore, Md. : 1950)195(8)3849-57(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LCMT-1 homozygous knock-out MEFs exhibited hyperphosphorylation of HDAC3, a reported target of the methylation-dependent PP4R1-PP4c complex. Collectively, our data suggest that LCMT-1 coordinately regulates the carboxyl methylation of PP2A-related phosphatases and, consequently, their holoenzyme assembly and function.
Title: Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.

T cell-specific ablation of PP4 resulted in defective adaptive immunity, impaired T cell homeostatic expansion, and inefficient T cell proliferation.
Title: T cell proliferation and adaptive immune responses are critically regulated by protein phosphatase 4.

Data show that protein phosphatase 4 (PP4) is an important regulator in inflammatory related insulin resistance.
Title: Protein phosphatase 4 (PP4) functions as a critical regulator in tumor necrosis factor (TNF)-?-induced hepatic insulin resistance.

Thus, serine/threonine phosphatase PP4 functions as a novel feedback negative regulator of RNA virus-triggered innate immunity.
Title: Phosphatase PP4 Negatively Regulates Type I IFN Production and Antiviral Innate Immunity by Dephosphorylating and Deactivating TBK1.

Taken together, our results establish a novel role for PP4 in CSR, and reveal crucial functions for PP4 in the maintenance of genomic stability, GC formation, and B cell-mediated immune responses.
Title: PP4 is essential for germinal center formation and class switch recombination in mice.

The following Ppp4c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp4c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu13508 NM_019674.4
Latest version!		 Mus musculus protein phosphatase 4, catalytic subunit (Ppp4c), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OMu13508 NM_001360464.1
Latest version!		 Mus musculus protein phosphatase 4, catalytic subunit (Ppp4c), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OMu39787 XM_006508049.2
Latest version!		 Mus musculus protein phosphatase 4, catalytic subunit (Ppp4c), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OMu39787 XM_006508049.1 Mus musculus protein phosphatase 4, catalytic subunit (Ppp4c), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OMu13508 NM_019674.3 Mus musculus protein phosphatase 4, catalytic subunit (Ppp4c), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu13508
Clone ID Related Accession (Same CDS sequence) NM_001360464.1 , NM_019674.3 , NM_019674.4
Accession Version NM_001360464.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 924bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-16
Organism Mus musculus(house mouse)
Product serine/threonine-protein phosphatase 4 catalytic subunit
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124505.4. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK144244.1, BI411046.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGCGGAGA TCAGCGACCT GGACCGGCAG ATCGAGCAGC TACGGCGCTG CGAGCTGATC 
AAAGAGAGCG AAGTCAAGGC CCTGTGCGCC AAGGCCAGAG AAATCTTGGT AGAAGAGAGC 
AACGTGCAGA GGGTGGACTC GCCAGTCACA GTATGCGGTG ACATCCATGG ACAATTCTAT 
GACCTCAAGG AGCTGTTCAG AGTAGGTGGC GATGTCCCTG AGACCAACTA CCTCTTCATG 
GGAGACTTTG TGGACCGTGG TTTCTACAGT GTTGAAACCT TCCTCCTCCT GCTGGCTCTT 
AAGGTTCGCT ATCCTGACAG AATCACTTTG ATCCGGGGCA ATCATGAGAG TCGCCAGATT 
ACCCAGGTCT ATGGGTTCTA TGATGAGTGC TTACGGAAAT ATGGTTCAGT GACTGTATGG 
CGCTACTGTA CTGAGATCTT TGACTACCTC AGCCTGTCTG CCATCATTGA TGGCAAGATC 
TTCTGTGTGC ATGGAGGTCT TTCCCCTTCC ATCCAGACCT TGGACCAGAT CCGGACAATT 
GACCGAAAGC AAGAGGTACC CCATGATGGA CCCATGTGCG ACCTCCTGTG GTCTGACCCT 
GAAGACACAA CAGGCTGGGG AGTGAGCCCC CGCGGGGCAG GTTACCTGTT TGGCAGTGAC 
GTGGTCGCCC AGTTCAATGC AGCCAACGAC ATTGATATGA TCTGCCGTGC CCACCAATTA 
GTGATGGAAG GCTACAAGTG GCACTTCAAT GAGACCGTGC TTACTGTGTG GTCAGCGCCT 
AATTACTGCT ACCGCTGTGG CAATGTGGCA GCCATCTTAG AACTGGATGA GCACCTCCAG 
AAAGATTTCA TCATCTTCGA GGCTGCACCC CAAGAGACAC GTGGCATCCC CTCCAAAAAG 
CCAGTGGCCG ACTATTTCCT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001347393.1
CDS207..1130
Translation

Target ORF information:

RefSeq Version NM_001360464.1
Organism Mus musculus(house mouse)
Definition Mus musculus protein phosphatase 4, catalytic subunit (Ppp4c), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001360464.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGCGGAGA TCAGCGACCT GGACCGGCAG ATCGAGCAGC TACGGCGCTG CGAGCTGATC 
AAAGAGAGCG AAGTCAAGGC CCTGTGCGCC AAGGCCAGAG AAATCTTGGT AGAAGAGAGC 
AACGTGCAGA GGGTGGACTC GCCAGTCACA GTATGCGGTG ACATCCATGG ACAATTCTAT 
GACCTCAAGG AGCTGTTCAG AGTAGGTGGC GATGTCCCTG AGACCAACTA CCTCTTCATG 
GGAGACTTTG TGGACCGTGG TTTCTACAGT GTTGAAACCT TCCTCCTCCT GCTGGCTCTT 
AAGGTTCGCT ATCCTGACAG AATCACTTTG ATCCGGGGCA ATCATGAGAG TCGCCAGATT 
ACCCAGGTCT ATGGGTTCTA TGATGAGTGC TTACGGAAAT ATGGTTCAGT GACTGTATGG 
CGCTACTGTA CTGAGATCTT TGACTACCTC AGCCTGTCTG CCATCATTGA TGGCAAGATC 
TTCTGTGTGC ATGGAGGTCT TTCCCCTTCC ATCCAGACCT TGGACCAGAT CCGGACAATT 
GACCGAAAGC AAGAGGTACC CCATGATGGA CCCATGTGCG ACCTCCTGTG GTCTGACCCT 
GAAGACACAA CAGGCTGGGG AGTGAGCCCC CGCGGGGCAG GTTACCTGTT TGGCAGTGAC 
GTGGTCGCCC AGTTCAATGC AGCCAACGAC ATTGATATGA TCTGCCGTGC CCACCAATTA 
GTGATGGAAG GCTACAAGTG GCACTTCAAT GAGACCGTGC TTACTGTGTG GTCAGCGCCT 
AATTACTGCT ACCGCTGTGG CAATGTGGCA GCCATCTTAG AACTGGATGA GCACCTCCAG 
AAAGATTTCA TCATCTTCGA GGCTGCACCC CAAGAGACAC GTGGCATCCC CTCCAAAAAG 
CCAGTGGCCG ACTATTTCCT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu13508
Clone ID Related Accession (Same CDS sequence) NM_001360464.1 , NM_019674.3 , NM_019674.4
Accession Version NM_019674.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 924bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-26
Organism Mus musculus(house mouse)
Product serine/threonine-protein phosphatase 4 catalytic subunit
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC124505.4. On Nov 17, 2006 this sequence version replaced NM_019674.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC001993.1, AF088911.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGCGGAGA TCAGCGACCT GGACCGGCAG ATCGAGCAGC TACGGCGCTG CGAGCTGATC 
AAAGAGAGCG AAGTCAAGGC CCTGTGCGCC AAGGCCAGAG AAATCTTGGT AGAAGAGAGC 
AACGTGCAGA GGGTGGACTC GCCAGTCACA GTATGCGGTG ACATCCATGG ACAATTCTAT 
GACCTCAAGG AGCTGTTCAG AGTAGGTGGC GATGTCCCTG AGACCAACTA CCTCTTCATG 
GGAGACTTTG TGGACCGTGG TTTCTACAGT GTTGAAACCT TCCTCCTCCT GCTGGCTCTT 
AAGGTTCGCT ATCCTGACAG AATCACTTTG ATCCGGGGCA ATCATGAGAG TCGCCAGATT 
ACCCAGGTCT ATGGGTTCTA TGATGAGTGC TTACGGAAAT ATGGTTCAGT GACTGTATGG 
CGCTACTGTA CTGAGATCTT TGACTACCTC AGCCTGTCTG CCATCATTGA TGGCAAGATC 
TTCTGTGTGC ATGGAGGTCT TTCCCCTTCC ATCCAGACCT TGGACCAGAT CCGGACAATT 
GACCGAAAGC AAGAGGTACC CCATGATGGA CCCATGTGCG ACCTCCTGTG GTCTGACCCT 
GAAGACACAA CAGGCTGGGG AGTGAGCCCC CGCGGGGCAG GTTACCTGTT TGGCAGTGAC 
GTGGTCGCCC AGTTCAATGC AGCCAACGAC ATTGATATGA TCTGCCGTGC CCACCAATTA 
GTGATGGAAG GCTACAAGTG GCACTTCAAT GAGACCGTGC TTACTGTGTG GTCAGCGCCT 
AATTACTGCT ACCGCTGTGG CAATGTGGCA GCCATCTTAG AACTGGATGA GCACCTCCAG 
AAAGATTTCA TCATCTTCGA GGCTGCACCC CAAGAGACAC GTGGCATCCC CTCCAAAAAG 
CCAGTGGCCG ACTATTTCCT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_062648.1
CDS115..1038
Translation

Target ORF information:

RefSeq Version NM_019674.3
Organism Mus musculus(house mouse)
Definition Mus musculus protein phosphatase 4, catalytic subunit (Ppp4c), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_019674.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGCGGAGA TCAGCGACCT GGACCGGCAG ATCGAGCAGC TACGGCGCTG CGAGCTGATC 
AAAGAGAGCG AAGTCAAGGC CCTGTGCGCC AAGGCCAGAG AAATCTTGGT AGAAGAGAGC 
AACGTGCAGA GGGTGGACTC GCCAGTCACA GTATGCGGTG ACATCCATGG ACAATTCTAT 
GACCTCAAGG AGCTGTTCAG AGTAGGTGGC GATGTCCCTG AGACCAACTA CCTCTTCATG 
GGAGACTTTG TGGACCGTGG TTTCTACAGT GTTGAAACCT TCCTCCTCCT GCTGGCTCTT 
AAGGTTCGCT ATCCTGACAG AATCACTTTG ATCCGGGGCA ATCATGAGAG TCGCCAGATT 
ACCCAGGTCT ATGGGTTCTA TGATGAGTGC TTACGGAAAT ATGGTTCAGT GACTGTATGG 
CGCTACTGTA CTGAGATCTT TGACTACCTC AGCCTGTCTG CCATCATTGA TGGCAAGATC 
TTCTGTGTGC ATGGAGGTCT TTCCCCTTCC ATCCAGACCT TGGACCAGAT CCGGACAATT 
GACCGAAAGC AAGAGGTACC CCATGATGGA CCCATGTGCG ACCTCCTGTG GTCTGACCCT 
GAAGACACAA CAGGCTGGGG AGTGAGCCCC CGCGGGGCAG GTTACCTGTT TGGCAGTGAC 
GTGGTCGCCC AGTTCAATGC AGCCAACGAC ATTGATATGA TCTGCCGTGC CCACCAATTA 
GTGATGGAAG GCTACAAGTG GCACTTCAAT GAGACCGTGC TTACTGTGTG GTCAGCGCCT 
AATTACTGCT ACCGCTGTGG CAATGTGGCA GCCATCTTAG AACTGGATGA GCACCTCCAG 
AAAGATTTCA TCATCTTCGA GGCTGCACCC CAAGAGACAC GTGGCATCCC CTCCAAAAAG 
CCAGTGGCCG ACTATTTCCT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu13508
Clone ID Related Accession (Same CDS sequence) NM_001360464.1 , NM_019674.3 , NM_019674.4
Accession Version NM_019674.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 924bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-22
Organism Mus musculus(house mouse)
Product serine/threonine-protein phosphatase 4 catalytic subunit
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124505.4. On Feb 3, 2018 this sequence version replaced NM_019674.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC001993.1, AF088911.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGCGGAGA TCAGCGACCT GGACCGGCAG ATCGAGCAGC TACGGCGCTG CGAGCTGATC 
AAAGAGAGCG AAGTCAAGGC CCTGTGCGCC AAGGCCAGAG AAATCTTGGT AGAAGAGAGC 
AACGTGCAGA GGGTGGACTC GCCAGTCACA GTATGCGGTG ACATCCATGG ACAATTCTAT 
GACCTCAAGG AGCTGTTCAG AGTAGGTGGC GATGTCCCTG AGACCAACTA CCTCTTCATG 
GGAGACTTTG TGGACCGTGG TTTCTACAGT GTTGAAACCT TCCTCCTCCT GCTGGCTCTT 
AAGGTTCGCT ATCCTGACAG AATCACTTTG ATCCGGGGCA ATCATGAGAG TCGCCAGATT 
ACCCAGGTCT ATGGGTTCTA TGATGAGTGC TTACGGAAAT ATGGTTCAGT GACTGTATGG 
CGCTACTGTA CTGAGATCTT TGACTACCTC AGCCTGTCTG CCATCATTGA TGGCAAGATC 
TTCTGTGTGC ATGGAGGTCT TTCCCCTTCC ATCCAGACCT TGGACCAGAT CCGGACAATT 
GACCGAAAGC AAGAGGTACC CCATGATGGA CCCATGTGCG ACCTCCTGTG GTCTGACCCT 
GAAGACACAA CAGGCTGGGG AGTGAGCCCC CGCGGGGCAG GTTACCTGTT TGGCAGTGAC 
GTGGTCGCCC AGTTCAATGC AGCCAACGAC ATTGATATGA TCTGCCGTGC CCACCAATTA 
GTGATGGAAG GCTACAAGTG GCACTTCAAT GAGACCGTGC TTACTGTGTG GTCAGCGCCT 
AATTACTGCT ACCGCTGTGG CAATGTGGCA GCCATCTTAG AACTGGATGA GCACCTCCAG 
AAAGATTTCA TCATCTTCGA GGCTGCACCC CAAGAGACAC GTGGCATCCC CTCCAAAAAG 
CCAGTGGCCG ACTATTTCCT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_062648.1
CDS191..1114
Translation

Target ORF information:

RefSeq Version NM_019674.4
Organism Mus musculus(house mouse)
Definition Mus musculus protein phosphatase 4, catalytic subunit (Ppp4c), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_019674.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGCGGAGA TCAGCGACCT GGACCGGCAG ATCGAGCAGC TACGGCGCTG CGAGCTGATC 
AAAGAGAGCG AAGTCAAGGC CCTGTGCGCC AAGGCCAGAG AAATCTTGGT AGAAGAGAGC 
AACGTGCAGA GGGTGGACTC GCCAGTCACA GTATGCGGTG ACATCCATGG ACAATTCTAT 
GACCTCAAGG AGCTGTTCAG AGTAGGTGGC GATGTCCCTG AGACCAACTA CCTCTTCATG 
GGAGACTTTG TGGACCGTGG TTTCTACAGT GTTGAAACCT TCCTCCTCCT GCTGGCTCTT 
AAGGTTCGCT ATCCTGACAG AATCACTTTG ATCCGGGGCA ATCATGAGAG TCGCCAGATT 
ACCCAGGTCT ATGGGTTCTA TGATGAGTGC TTACGGAAAT ATGGTTCAGT GACTGTATGG 
CGCTACTGTA CTGAGATCTT TGACTACCTC AGCCTGTCTG CCATCATTGA TGGCAAGATC 
TTCTGTGTGC ATGGAGGTCT TTCCCCTTCC ATCCAGACCT TGGACCAGAT CCGGACAATT 
GACCGAAAGC AAGAGGTACC CCATGATGGA CCCATGTGCG ACCTCCTGTG GTCTGACCCT 
GAAGACACAA CAGGCTGGGG AGTGAGCCCC CGCGGGGCAG GTTACCTGTT TGGCAGTGAC 
GTGGTCGCCC AGTTCAATGC AGCCAACGAC ATTGATATGA TCTGCCGTGC CCACCAATTA 
GTGATGGAAG GCTACAAGTG GCACTTCAAT GAGACCGTGC TTACTGTGTG GTCAGCGCCT 
AATTACTGCT ACCGCTGTGG CAATGTGGCA GCCATCTTAG AACTGGATGA GCACCTCCAG 
AAAGATTTCA TCATCTTCGA GGCTGCACCC CAAGAGACAC GTGGCATCCC CTCCAAAAAG 
CCAGTGGCCG ACTATTTCCT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu39787
Clone ID Related Accession (Same CDS sequence) XM_006508049.1 , XM_006508049.2
Accession Version XM_006508049.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 687bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product serine/threonine-protein phosphatase 4 catalytic subunit isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
ATGGGAGACT TTGTGGACCG TGGTTTCTAC AGTGTTGAAA CCTTCCTCCT CCTGCTGGCT 
CTTAAGGTTC GCTATCCTGA CAGAATCACT TTGATCCGGG GCAATCATGA GAGTCGCCAG 
ATTACCCAGG TCTATGGGTT CTATGATGAG TGCTTACGGA AATATGGTTC AGTGACTGTA 
TGGCGCTACT GTACTGAGAT CTTTGACTAC CTCAGCCTGT CTGCCATCAT TGATGGCAAG 
ATCTTCTGTG TGCATGGAGG TCTTTCCCCT TCCATCCAGA CCTTGGACCA GATCCGGACA 
ATTGACCGAA AGCAAGAGGT ACCCCATGAT GGACCCATGT GCGACCTCCT GTGGTCTGAC 
CCTGAAGACA CAACAGGCTG GGGAGTGAGC CCCCGCGGGG CAGGTTACCT GTTTGGCAGT 
GACGTGGTCG CCCAGTTCAA TGCAGCCAAC GACATTGATA TGATCTGCCG TGCCCACCAA 
TTAGTGATGG AAGGCTACAA GTGGCACTTC AATGAGACCG TGCTTACTGT GTGGTCAGCG 
CCTAATTACT GCTACCGCTG TGGCAATGTG GCAGCCATCT TAGAACTGGA TGAGCACCTC 
CAGAAAGATT TCATCATCTT CGAGGCTGCA CCCCAAGAGA CACGTGGCAT CCCCTCCAAA 
AAGCCAGTGG CCGACTATTT CCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006508112.1
CDS286..972
Translation

Target ORF information:

RefSeq Version XM_006508049.1
Organism Mus musculus(house mouse)
Definition Mus musculus protein phosphatase 4, catalytic subunit (Ppp4c), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006508049.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGGAGACT TTGTGGACCG TGGTTTCTAC AGTGTTGAAA CCTTCCTCCT CCTGCTGGCT 
CTTAAGGTTC GCTATCCTGA CAGAATCACT TTGATCCGGG GCAATCATGA GAGTCGCCAG 
ATTACCCAGG TCTATGGGTT CTATGATGAG TGCTTACGGA AATATGGTTC AGTGACTGTA 
TGGCGCTACT GTACTGAGAT CTTTGACTAC CTCAGCCTGT CTGCCATCAT TGATGGCAAG 
ATCTTCTGTG TGCATGGAGG TCTTTCCCCT TCCATCCAGA CCTTGGACCA GATCCGGACA 
ATTGACCGAA AGCAAGAGGT ACCCCATGAT GGACCCATGT GCGACCTCCT GTGGTCTGAC 
CCTGAAGACA CAACAGGCTG GGGAGTGAGC CCCCGCGGGG CAGGTTACCT GTTTGGCAGT 
GACGTGGTCG CCCAGTTCAA TGCAGCCAAC GACATTGATA TGATCTGCCG TGCCCACCAA 
TTAGTGATGG AAGGCTACAA GTGGCACTTC AATGAGACCG TGCTTACTGT GTGGTCAGCG 
CCTAATTACT GCTACCGCTG TGGCAATGTG GCAGCCATCT TAGAACTGGA TGAGCACCTC 
CAGAAAGATT TCATCATCTT CGAGGCTGCA CCCCAAGAGA CACGTGGCAT CCCCTCCAAA 
AAGCCAGTGG CCGACTATTT CCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu39787
Clone ID Related Accession (Same CDS sequence) XM_006508049.1 , XM_006508049.2
Accession Version XM_006508049.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 687bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product serine/threonine-protein phosphatase 4 catalytic subunit isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
ATGGGAGACT TTGTGGACCG TGGTTTCTAC AGTGTTGAAA CCTTCCTCCT CCTGCTGGCT 
CTTAAGGTTC GCTATCCTGA CAGAATCACT TTGATCCGGG GCAATCATGA GAGTCGCCAG 
ATTACCCAGG TCTATGGGTT CTATGATGAG TGCTTACGGA AATATGGTTC AGTGACTGTA 
TGGCGCTACT GTACTGAGAT CTTTGACTAC CTCAGCCTGT CTGCCATCAT TGATGGCAAG 
ATCTTCTGTG TGCATGGAGG TCTTTCCCCT TCCATCCAGA CCTTGGACCA GATCCGGACA 
ATTGACCGAA AGCAAGAGGT ACCCCATGAT GGACCCATGT GCGACCTCCT GTGGTCTGAC 
CCTGAAGACA CAACAGGCTG GGGAGTGAGC CCCCGCGGGG CAGGTTACCT GTTTGGCAGT 
GACGTGGTCG CCCAGTTCAA TGCAGCCAAC GACATTGATA TGATCTGCCG TGCCCACCAA 
TTAGTGATGG AAGGCTACAA GTGGCACTTC AATGAGACCG TGCTTACTGT GTGGTCAGCG 
CCTAATTACT GCTACCGCTG TGGCAATGTG GCAGCCATCT TAGAACTGGA TGAGCACCTC 
CAGAAAGATT TCATCATCTT CGAGGCTGCA CCCCAAGAGA CACGTGGCAT CCCCTCCAAA 
AAGCCAGTGG CCGACTATTT CCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006508112.1
CDS497..1183
Translation

Target ORF information:

RefSeq Version XM_006508049.2
Organism Mus musculus(house mouse)
Definition Mus musculus protein phosphatase 4, catalytic subunit (Ppp4c), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006508049.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGGAGACT TTGTGGACCG TGGTTTCTAC AGTGTTGAAA CCTTCCTCCT CCTGCTGGCT 
CTTAAGGTTC GCTATCCTGA CAGAATCACT TTGATCCGGG GCAATCATGA GAGTCGCCAG 
ATTACCCAGG TCTATGGGTT CTATGATGAG TGCTTACGGA AATATGGTTC AGTGACTGTA 
TGGCGCTACT GTACTGAGAT CTTTGACTAC CTCAGCCTGT CTGCCATCAT TGATGGCAAG 
ATCTTCTGTG TGCATGGAGG TCTTTCCCCT TCCATCCAGA CCTTGGACCA GATCCGGACA 
ATTGACCGAA AGCAAGAGGT ACCCCATGAT GGACCCATGT GCGACCTCCT GTGGTCTGAC 
CCTGAAGACA CAACAGGCTG GGGAGTGAGC CCCCGCGGGG CAGGTTACCT GTTTGGCAGT 
GACGTGGTCG CCCAGTTCAA TGCAGCCAAC GACATTGATA TGATCTGCCG TGCCCACCAA 
TTAGTGATGG AAGGCTACAA GTGGCACTTC AATGAGACCG TGCTTACTGT GTGGTCAGCG 
CCTAATTACT GCTACCGCTG TGGCAATGTG GCAGCCATCT TAGAACTGGA TGAGCACCTC 
CAGAAAGATT TCATCATCTT CGAGGCTGCA CCCCAAGAGA CACGTGGCAT CCCCTCCAAA 
AAGCCAGTGG CCGACTATTT CCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.
The Journal of biological chemistry291(40)21008-21019(2016 Sep)
Hwang J,Lee JA,Pallas DC


book

T cell proliferation and adaptive immune responses are critically regulated by protein phosphatase 4.
Cell cycle (Georgetown, Tex.)15(8)1073-83(2016)
Liao FH,Hsiao WY,Lin YC,Chan YC,Huang CY


book

Protein phosphatase 4 (PP4) functions as a critical regulator in tumor necrosis factor (TNF)-?-induced hepatic insulin resistance.
Scientific reports518093(2015 Dec)
Zhao H,Huang X,Jiao J,Zhang H,Liu J,Qin W,Meng X,Shen T,Lin Y,Chu J,Li J


book

Phosphatase PP4 Negatively Regulates Type I IFN Production and Antiviral Innate Immunity by Dephosphorylating and Deactivating TBK1.
Journal of immunology (Baltimore, Md. : 1950)195(8)3849-57(2015 Oct)
Zhan Z,Cao H,Xie X,Yang L,Zhang P,Chen Y,Fan H,Liu Z,Liu X