Ncstn cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Ncstn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncstn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu77247 XM_017321872.2
Latest version!
Mus musculus nicastrin (Ncstn), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu17605 NM_021607.3
Latest version!
Mus musculus nicastrin (Ncstn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu28821 XM_006496952.4
Latest version!
Mus musculus nicastrin (Ncstn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OMu77247 XM_017321872.1 Mus musculus nicastrin (Ncstn), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu28821 XM_006496952.3 Mus musculus nicastrin (Ncstn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu77247
    Clone ID Related Accession (Same CDS sequence) XM_017321872.2 , XM_017321872.1
    Accession Version XM_017321872.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product nicastrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCAGCTCT TCTCACACAT GCACGCCGTC ATCAGCACTG CCACCTGCAT GCGGCGCAGC 
    TTCATCCAGA GCACCTTCAG CATCAACCCA GAAATCGTCT GTGACCCCTT ATCCGACTAC
    AACGTATGGA GCATGCTTAA GCCTATAAAT ACATCTGTGG GACTAGAACC TGACGTCAGG
    GTTGTGGTTG CGGCCACACG GCTGGATAGC CGGTCCTTTT TCTGGAATGT GGCCCCAGGG
    GCTGAAAGTG CTGTAGCCTC CTTTGTCACT CAGCTGGCTG CAGCTGAAGC TTTGCACAAG
    GCACCTGATG TGACCACTCT ATCCCGAAAT GTGATGTTTG TCTTCTTCCA GGGGGAAACT
    TTTGACTACA TTGGCAGCTC ACGGATGGTC TATGATATGG AGAACGGCAA GTTTCCCGTG
    CGGCTCGAGA ACATTGACTC CTTCGTGGAG CTGGGACAGG TGGCCCTAAG AACTTCACTA
    GATCTCTGGA TGCACACAGA TCCCATGTCT CAGAAAAATG AGTCTGTGAA AAACCAGGTG
    GAGGATCTTC TGGCCACTCT GGAGAAGAGC GGTGCTGGTG TCCCTGAAGT TGTCCTGAGG
    AGACTGGCCC AGTCCCAGGC CCTTCCACCT TCGTCTCTAC AACGATTTCT TCGGGCTCGA
    AACATCTCTG GCGTGGTCCT GGCTGACCAC TCTGGCTCCT TCCACAATCG GTATTACCAG
    AGCATTTATG ACACGGCTGA GAACATTAAT GTGACCTATC CTGAGTGGCA GAGCCCAGAA
    GAGGACCTCA ACTTTGTGAC AGACACTGCC AAGGCACTGG CGAATGTGGC CACAGTGCTG
    GCGCGTGCAC TGTATGAGCT TGCAGGAGGA ACCAACTTCA GCAGCTCCAT CCAGGCTGAT
    CCCCAGACAG TTACACGTCT GCTCTATGGG TTCCTGGTTA GAGCTAACAA CTCATGGTTT
    CAGTCGATCT TGAAACATGA CCTAAGGTCC TATTTGGATG ACAGGCCTCT TCAACACTAC
    ATCGCCGTCT CCAGCCCTAC CAACACGACT TACGTTGTGC AGTACGCCTT GGCAAACCTG
    ACTGGCAAGG CGACCAACCT CACCCGAGAG CAGTGCCAGG ATCCAAGTAA AGTCCCAAAT
    GAGAGCAAGG ATTTATATGA ATACTCGTGG GTACAAGGCC CTTGGAATTC CAACAGGACA
    GAGAGGCTCC CACAGTGTGT GCGCTCCACA GTGCGACTGG CCAGGGCCTT GTCCCCTGCC
    TTTGAACTGA GTCAGTGGAG CTCCACAGAA TACTCTACGT GGGCGGAGAG CCGCTGGAAA
    GACATCCAAG CTCGGATATT CCTAATTGCC AGCAAAGAGC TTGAGTTCAT CACGCTGATC
    GTGGGCTTCA GCACCCTTGT CTTCTCCCTC ATCGTCACCT ACTGCATCAA TGCCAAAGCC
    GACGTCCTTT TCGTTGCTCC CCGAGAGCCA GGAGCTGTGT CTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177361.1
    CDS653..2140
    Translation

    Target ORF information:

    RefSeq Version XM_017321872.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus nicastrin (Ncstn), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCAGCTCT TCTCACACAT GCACGCCGTC ATCAGCACTG CCACCTGCAT GCGGCGCAGC 
    TTCATCCAGA GCACCTTCAG CATCAACCCA GAAATCGTCT GTGACCCCTT ATCCGACTAC
    AACGTATGGA GCATGCTTAA GCCTATAAAT ACATCTGTGG GACTAGAACC TGACGTCAGG
    GTTGTGGTTG CGGCCACACG GCTGGATAGC CGGTCCTTTT TCTGGAATGT GGCCCCAGGG
    GCTGAAAGTG CTGTAGCCTC CTTTGTCACT CAGCTGGCTG CAGCTGAAGC TTTGCACAAG
    GCACCTGATG TGACCACTCT ATCCCGAAAT GTGATGTTTG TCTTCTTCCA GGGGGAAACT
    TTTGACTACA TTGGCAGCTC ACGGATGGTC TATGATATGG AGAACGGCAA GTTTCCCGTG
    CGGCTCGAGA ACATTGACTC CTTCGTGGAG CTGGGACAGG TGGCCCTAAG AACTTCACTA
    GATCTCTGGA TGCACACAGA TCCCATGTCT CAGAAAAATG AGTCTGTGAA AAACCAGGTG
    GAGGATCTTC TGGCCACTCT GGAGAAGAGC GGTGCTGGTG TCCCTGAAGT TGTCCTGAGG
    AGACTGGCCC AGTCCCAGGC CCTTCCACCT TCGTCTCTAC AACGATTTCT TCGGGCTCGA
    AACATCTCTG GCGTGGTCCT GGCTGACCAC TCTGGCTCCT TCCACAATCG GTATTACCAG
    AGCATTTATG ACACGGCTGA GAACATTAAT GTGACCTATC CTGAGTGGCA GAGCCCAGAA
    GAGGACCTCA ACTTTGTGAC AGACACTGCC AAGGCACTGG CGAATGTGGC CACAGTGCTG
    GCGCGTGCAC TGTATGAGCT TGCAGGAGGA ACCAACTTCA GCAGCTCCAT CCAGGCTGAT
    CCCCAGACAG TTACACGTCT GCTCTATGGG TTCCTGGTTA GAGCTAACAA CTCATGGTTT
    CAGTCGATCT TGAAACATGA CCTAAGGTCC TATTTGGATG ACAGGCCTCT TCAACACTAC
    ATCGCCGTCT CCAGCCCTAC CAACACGACT TACGTTGTGC AGTACGCCTT GGCAAACCTG
    ACTGGCAAGG CGACCAACCT CACCCGAGAG CAGTGCCAGG ATCCAAGTAA AGTCCCAAAT
    GAGAGCAAGG ATTTATATGA ATACTCGTGG GTACAAGGCC CTTGGAATTC CAACAGGACA
    GAGAGGCTCC CACAGTGTGT GCGCTCCACA GTGCGACTGG CCAGGGCCTT GTCCCCTGCC
    TTTGAACTGA GTCAGTGGAG CTCCACAGAA TACTCTACGT GGGCGGAGAG CCGCTGGAAA
    GACATCCAAG CTCGGATATT CCTAATTGCC AGCAAAGAGC TTGAGTTCAT CACGCTGATC
    GTGGGCTTCA GCACCCTTGT CTTCTCCCTC ATCGTCACCT ACTGCATCAA TGCCAAAGCC
    GACGTCCTTT TCGTTGCTCC CCGAGAGCCA GGAGCTGTGT CTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77247
    Clone ID Related Accession (Same CDS sequence) XM_017321872.2 , XM_017321872.1
    Accession Version XM_017321872.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product nicastrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCAGCTCT TCTCACACAT GCACGCCGTC ATCAGCACTG CCACCTGCAT GCGGCGCAGC 
    TTCATCCAGA GCACCTTCAG CATCAACCCA GAAATCGTCT GTGACCCCTT ATCCGACTAC
    AACGTATGGA GCATGCTTAA GCCTATAAAT ACATCTGTGG GACTAGAACC TGACGTCAGG
    GTTGTGGTTG CGGCCACACG GCTGGATAGC CGGTCCTTTT TCTGGAATGT GGCCCCAGGG
    GCTGAAAGTG CTGTAGCCTC CTTTGTCACT CAGCTGGCTG CAGCTGAAGC TTTGCACAAG
    GCACCTGATG TGACCACTCT ATCCCGAAAT GTGATGTTTG TCTTCTTCCA GGGGGAAACT
    TTTGACTACA TTGGCAGCTC ACGGATGGTC TATGATATGG AGAACGGCAA GTTTCCCGTG
    CGGCTCGAGA ACATTGACTC CTTCGTGGAG CTGGGACAGG TGGCCCTAAG AACTTCACTA
    GATCTCTGGA TGCACACAGA TCCCATGTCT CAGAAAAATG AGTCTGTGAA AAACCAGGTG
    GAGGATCTTC TGGCCACTCT GGAGAAGAGC GGTGCTGGTG TCCCTGAAGT TGTCCTGAGG
    AGACTGGCCC AGTCCCAGGC CCTTCCACCT TCGTCTCTAC AACGATTTCT TCGGGCTCGA
    AACATCTCTG GCGTGGTCCT GGCTGACCAC TCTGGCTCCT TCCACAATCG GTATTACCAG
    AGCATTTATG ACACGGCTGA GAACATTAAT GTGACCTATC CTGAGTGGCA GAGCCCAGAA
    GAGGACCTCA ACTTTGTGAC AGACACTGCC AAGGCACTGG CGAATGTGGC CACAGTGCTG
    GCGCGTGCAC TGTATGAGCT TGCAGGAGGA ACCAACTTCA GCAGCTCCAT CCAGGCTGAT
    CCCCAGACAG TTACACGTCT GCTCTATGGG TTCCTGGTTA GAGCTAACAA CTCATGGTTT
    CAGTCGATCT TGAAACATGA CCTAAGGTCC TATTTGGATG ACAGGCCTCT TCAACACTAC
    ATCGCCGTCT CCAGCCCTAC CAACACGACT TACGTTGTGC AGTACGCCTT GGCAAACCTG
    ACTGGCAAGG CGACCAACCT CACCCGAGAG CAGTGCCAGG ATCCAAGTAA AGTCCCAAAT
    GAGAGCAAGG ATTTATATGA ATACTCGTGG GTACAAGGCC CTTGGAATTC CAACAGGACA
    GAGAGGCTCC CACAGTGTGT GCGCTCCACA GTGCGACTGG CCAGGGCCTT GTCCCCTGCC
    TTTGAACTGA GTCAGTGGAG CTCCACAGAA TACTCTACGT GGGCGGAGAG CCGCTGGAAA
    GACATCCAAG CTCGGATATT CCTAATTGCC AGCAAAGAGC TTGAGTTCAT CACGCTGATC
    GTGGGCTTCA GCACCCTTGT CTTCTCCCTC ATCGTCACCT ACTGCATCAA TGCCAAAGCC
    GACGTCCTTT TCGTTGCTCC CCGAGAGCCA GGAGCTGTGT CTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017177361.1
    CDS784..2271
    Translation

    Target ORF information:

    RefSeq Version XM_017321872.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus nicastrin (Ncstn), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321872.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCAGCTCT TCTCACACAT GCACGCCGTC ATCAGCACTG CCACCTGCAT GCGGCGCAGC 
    TTCATCCAGA GCACCTTCAG CATCAACCCA GAAATCGTCT GTGACCCCTT ATCCGACTAC
    AACGTATGGA GCATGCTTAA GCCTATAAAT ACATCTGTGG GACTAGAACC TGACGTCAGG
    GTTGTGGTTG CGGCCACACG GCTGGATAGC CGGTCCTTTT TCTGGAATGT GGCCCCAGGG
    GCTGAAAGTG CTGTAGCCTC CTTTGTCACT CAGCTGGCTG CAGCTGAAGC TTTGCACAAG
    GCACCTGATG TGACCACTCT ATCCCGAAAT GTGATGTTTG TCTTCTTCCA GGGGGAAACT
    TTTGACTACA TTGGCAGCTC ACGGATGGTC TATGATATGG AGAACGGCAA GTTTCCCGTG
    CGGCTCGAGA ACATTGACTC CTTCGTGGAG CTGGGACAGG TGGCCCTAAG AACTTCACTA
    GATCTCTGGA TGCACACAGA TCCCATGTCT CAGAAAAATG AGTCTGTGAA AAACCAGGTG
    GAGGATCTTC TGGCCACTCT GGAGAAGAGC GGTGCTGGTG TCCCTGAAGT TGTCCTGAGG
    AGACTGGCCC AGTCCCAGGC CCTTCCACCT TCGTCTCTAC AACGATTTCT TCGGGCTCGA
    AACATCTCTG GCGTGGTCCT GGCTGACCAC TCTGGCTCCT TCCACAATCG GTATTACCAG
    AGCATTTATG ACACGGCTGA GAACATTAAT GTGACCTATC CTGAGTGGCA GAGCCCAGAA
    GAGGACCTCA ACTTTGTGAC AGACACTGCC AAGGCACTGG CGAATGTGGC CACAGTGCTG
    GCGCGTGCAC TGTATGAGCT TGCAGGAGGA ACCAACTTCA GCAGCTCCAT CCAGGCTGAT
    CCCCAGACAG TTACACGTCT GCTCTATGGG TTCCTGGTTA GAGCTAACAA CTCATGGTTT
    CAGTCGATCT TGAAACATGA CCTAAGGTCC TATTTGGATG ACAGGCCTCT TCAACACTAC
    ATCGCCGTCT CCAGCCCTAC CAACACGACT TACGTTGTGC AGTACGCCTT GGCAAACCTG
    ACTGGCAAGG CGACCAACCT CACCCGAGAG CAGTGCCAGG ATCCAAGTAA AGTCCCAAAT
    GAGAGCAAGG ATTTATATGA ATACTCGTGG GTACAAGGCC CTTGGAATTC CAACAGGACA
    GAGAGGCTCC CACAGTGTGT GCGCTCCACA GTGCGACTGG CCAGGGCCTT GTCCCCTGCC
    TTTGAACTGA GTCAGTGGAG CTCCACAGAA TACTCTACGT GGGCGGAGAG CCGCTGGAAA
    GACATCCAAG CTCGGATATT CCTAATTGCC AGCAAAGAGC TTGAGTTCAT CACGCTGATC
    GTGGGCTTCA GCACCCTTGT CTTCTCCCTC ATCGTCACCT ACTGCATCAA TGCCAAAGCC
    GACGTCCTTT TCGTTGCTCC CCGAGAGCCA GGAGCTGTGT CTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17605
    Clone ID Related Accession (Same CDS sequence) NM_021607.3
    Accession Version NM_021607.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product nicastrin precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CD549803.1, AK033314.1 and BF714603.1. On Mar 11, 2009 this sequence version replaced NM_021607.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129102.1, AK033314.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTACGA CTAGGGGCGG CTCTGGGCCT GACCCAGGAA GTCGGGGTCT TCTTCTTCTG 
    TCTTTTTCCG TGGTACTGGC AGGATTGTGT GGGGGAAACT CAGTGGAGAG GAAAATCTAC
    ATTCCCTTAA ATAAAACAGC TCCTTGTGTC CGCCTGCTCA ACGCCACTCA TCAGATTGGC
    TGCCAGTCTT CAATTAGTGG GGATACAGGG GTTATCCATG TAGTGGAGAA AGAAGAAGAC
    CTGAAGTGGG TGTTGACCGA TGGCCCCAAC CCCCCTTACA TGGTTCTGCT GGAGGGCAAG
    CTCTTCACCA GAGATGTAAT GGAGAAGCTG AAGGGAACAA CCAGTAGAAT CGCTGGTCTT
    GCCGTGACTC TAGCCAAGCC CAACTCAACT TCAAGCTTCT CTCCTAGTGT GCAGTGCCCA
    AATGATGGGT TTGGTATTTA CTCCAACTCC TACGGGCCAG AGTTTGCTCA CTGCAAGAAA
    ACACTGTGGA ATGAACTGGG CAACGGCTTG GCTTATGAAG ACTTTAGTTT CCCCATCTTT
    CTTCTTGAAG ATGAGAACGA AACCAAGGTC ATCAAGCAGT GCTATCAAGA TCACAACCTG
    GGTCAGAATG GCTCTGCACC AAGCTTCCCA TTGTGTGCTA TGCAGCTCTT CTCACACATG
    CACGCCGTCA TCAGCACTGC CACCTGCATG CGGCGCAGCT TCATCCAGAG CACCTTCAGC
    ATCAACCCAG AAATCGTCTG TGACCCCTTA TCCGACTACA ACGTATGGAG CATGCTTAAG
    CCTATAAATA CATCTGTGGG ACTAGAACCT GACGTCAGGG TTGTGGTTGC GGCCACACGG
    CTGGATAGCC GGTCCTTTTT CTGGAATGTG GCCCCAGGGG CTGAAAGTGC TGTAGCCTCC
    TTTGTCACTC AGCTGGCTGC AGCTGAAGCT TTGCACAAGG CACCTGATGT GACCACTCTA
    TCCCGAAATG TGATGTTTGT CTTCTTCCAG GGGGAAACTT TTGACTACAT TGGCAGCTCA
    CGGATGGTCT ATGATATGGA GAACGGCAAG TTTCCCGTGC GGCTCGAGAA CATTGACTCC
    TTCGTGGAGC TGGGACAGGT GGCCCTAAGA ACTTCACTAG ATCTCTGGAT GCACACAGAT
    CCCATGTCTC AGAAAAATGA GTCTGTGAAA AACCAGGTGG AGGATCTTCT GGCCACTCTG
    GAGAAGAGCG GTGCTGGTGT CCCTGAAGTT GTCCTGAGGA GACTGGCCCA GTCCCAGGCC
    CTTCCACCTT CGTCTCTACA ACGATTTCTT CGGGCTCGAA ACATCTCTGG CGTGGTCCTG
    GCTGACCACT CTGGCTCCTT CCACAATCGG TATTACCAGA GCATTTATGA CACGGCTGAG
    AACATTAATG TGACCTATCC TGAGTGGCAG AGCCCAGAAG AGGACCTCAA CTTTGTGACA
    GACACTGCCA AGGCACTGGC GAATGTGGCC ACAGTGCTGG CGCGTGCACT GTATGAGCTT
    GCAGGAGGAA CCAACTTCAG CAGCTCCATC CAGGCTGATC CCCAGACAGT TACACGTCTG
    CTCTATGGGT TCCTGGTTAG AGCTAACAAC TCATGGTTTC AGTCGATCTT GAAACATGAC
    CTAAGGTCCT ATTTGGATGA CAGGCCTCTT CAACACTACA TCGCCGTCTC CAGCCCTACC
    AACACGACTT ACGTTGTGCA GTACGCCTTG GCAAACCTGA CTGGCAAGGC GACCAACCTC
    ACCCGAGAGC AGTGCCAGGA TCCAAGTAAA GTCCCAAATG AGAGCAAGGA TTTATATGAA
    TACTCGTGGG TACAAGGCCC TTGGAATTCC AACAGGACAG AGAGGCTCCC ACAGTGTGTG
    CGCTCCACAG TGCGACTGGC CAGGGCCTTG TCCCCTGCCT TTGAACTGAG TCAGTGGAGC
    TCCACAGAAT ACTCTACGTG GGCGGAGAGC CGCTGGAAAG ACATCCAAGC TCGGATATTC
    CTAATTGCCA GCAAAGAGCT TGAGTTCATC ACGCTGATCG TGGGCTTCAG CACCCTTGTC
    TTCTCCCTCA TCGTCACCTA CTGCATCAAT GCCAAAGCCG ACGTCCTTTT CGTTGCTCCC
    CGAGAGCCAG GAGCTGTGTC TTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_067620.3
    CDS68..2194
    Translation

    Target ORF information:

    RefSeq Version NM_021607.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus nicastrin (Ncstn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_021607.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTACGA CTAGGGGCGG CTCTGGGCCT GACCCAGGAA GTCGGGGTCT TCTTCTTCTG 
    TCTTTTTCCG TGGTACTGGC AGGATTGTGT GGGGGAAACT CAGTGGAGAG GAAAATCTAC
    ATTCCCTTAA ATAAAACAGC TCCTTGTGTC CGCCTGCTCA ACGCCACTCA TCAGATTGGC
    TGCCAGTCTT CAATTAGTGG GGATACAGGG GTTATCCATG TAGTGGAGAA AGAAGAAGAC
    CTGAAGTGGG TGTTGACCGA TGGCCCCAAC CCCCCTTACA TGGTTCTGCT GGAGGGCAAG
    CTCTTCACCA GAGATGTAAT GGAGAAGCTG AAGGGAACAA CCAGTAGAAT CGCTGGTCTT
    GCCGTGACTC TAGCCAAGCC CAACTCAACT TCAAGCTTCT CTCCTAGTGT GCAGTGCCCA
    AATGATGGGT TTGGTATTTA CTCCAACTCC TACGGGCCAG AGTTTGCTCA CTGCAAGAAA
    ACACTGTGGA ATGAACTGGG CAACGGCTTG GCTTATGAAG ACTTTAGTTT CCCCATCTTT
    CTTCTTGAAG ATGAGAACGA AACCAAGGTC ATCAAGCAGT GCTATCAAGA TCACAACCTG
    GGTCAGAATG GCTCTGCACC AAGCTTCCCA TTGTGTGCTA TGCAGCTCTT CTCACACATG
    CACGCCGTCA TCAGCACTGC CACCTGCATG CGGCGCAGCT TCATCCAGAG CACCTTCAGC
    ATCAACCCAG AAATCGTCTG TGACCCCTTA TCCGACTACA ACGTATGGAG CATGCTTAAG
    CCTATAAATA CATCTGTGGG ACTAGAACCT GACGTCAGGG TTGTGGTTGC GGCCACACGG
    CTGGATAGCC GGTCCTTTTT CTGGAATGTG GCCCCAGGGG CTGAAAGTGC TGTAGCCTCC
    TTTGTCACTC AGCTGGCTGC AGCTGAAGCT TTGCACAAGG CACCTGATGT GACCACTCTA
    TCCCGAAATG TGATGTTTGT CTTCTTCCAG GGGGAAACTT TTGACTACAT TGGCAGCTCA
    CGGATGGTCT ATGATATGGA GAACGGCAAG TTTCCCGTGC GGCTCGAGAA CATTGACTCC
    TTCGTGGAGC TGGGACAGGT GGCCCTAAGA ACTTCACTAG ATCTCTGGAT GCACACAGAT
    CCCATGTCTC AGAAAAATGA GTCTGTGAAA AACCAGGTGG AGGATCTTCT GGCCACTCTG
    GAGAAGAGCG GTGCTGGTGT CCCTGAAGTT GTCCTGAGGA GACTGGCCCA GTCCCAGGCC
    CTTCCACCTT CGTCTCTACA ACGATTTCTT CGGGCTCGAA ACATCTCTGG CGTGGTCCTG
    GCTGACCACT CTGGCTCCTT CCACAATCGG TATTACCAGA GCATTTATGA CACGGCTGAG
    AACATTAATG TGACCTATCC TGAGTGGCAG AGCCCAGAAG AGGACCTCAA CTTTGTGACA
    GACACTGCCA AGGCACTGGC GAATGTGGCC ACAGTGCTGG CGCGTGCACT GTATGAGCTT
    GCAGGAGGAA CCAACTTCAG CAGCTCCATC CAGGCTGATC CCCAGACAGT TACACGTCTG
    CTCTATGGGT TCCTGGTTAG AGCTAACAAC TCATGGTTTC AGTCGATCTT GAAACATGAC
    CTAAGGTCCT ATTTGGATGA CAGGCCTCTT CAACACTACA TCGCCGTCTC CAGCCCTACC
    AACACGACTT ACGTTGTGCA GTACGCCTTG GCAAACCTGA CTGGCAAGGC GACCAACCTC
    ACCCGAGAGC AGTGCCAGGA TCCAAGTAAA GTCCCAAATG AGAGCAAGGA TTTATATGAA
    TACTCGTGGG TACAAGGCCC TTGGAATTCC AACAGGACAG AGAGGCTCCC ACAGTGTGTG
    CGCTCCACAG TGCGACTGGC CAGGGCCTTG TCCCCTGCCT TTGAACTGAG TCAGTGGAGC
    TCCACAGAAT ACTCTACGTG GGCGGAGAGC CGCTGGAAAG ACATCCAAGC TCGGATATTC
    CTAATTGCCA GCAAAGAGCT TGAGTTCATC ACGCTGATCG TGGGCTTCAG CACCCTTGTC
    TTCTCCCTCA TCGTCACCTA CTGCATCAAT GCCAAAGCCG ACGTCCTTTT CGTTGCTCCC
    CGAGAGCCAG GAGCTGTGTC TTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28821
    Clone ID Related Accession (Same CDS sequence) XM_006496952.4 , XM_006496952.3
    Accession Version XM_006496952.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product nicastrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCCTCGTT CTCTGTTTAA AGCCCAGCCA CCCCATCAGC CATCAAAAGG GACAGGATTG 
    TGTGGGGGAA ACTCAGTGGA GAGGAAAATC TACATTCCCT TAAATAAAAC AGCTCCTTGT
    GTCCGCCTGC TCAACGCCAC TCATCAGATT GGCTGCCAGT CTTCAATTAG TGGGGATACA
    GGGGTTATCC ATGTAGTGGA GAAAGAAGAA GACCTGAAGT GGGTGTTGAC CGATGGCCCC
    AACCCCCCTT ACATGGTTCT GCTGGAGGGC AAGCTCTTCA CCAGAGATGT AATGGAGAAG
    CTGAAGGGAA CAACCAGTAG AATCGCTGGT CTTGCCGTGA CTCTAGCCAA GCCCAACTCA
    ACTTCAAGCT TCTCTCCTAG TGTGCAGTGC CCAAATGATG GGTTTGGTAT TTACTCCAAC
    TCCTACGGGC CAGAGTTTGC TCACTGCAAG AAAACACTGT GGAATGAACT GGGCAACGGC
    TTGGCTTATG AAGACTTTAG TTTCCCCATC TTTCTTCTTG AAGATGAGAA CGAAACCAAG
    GTCATCAAGC AGTGCTATCA AGATCACAAC CTGGGTCAGA ATGGCTCTGC ACCAAGCTTC
    CCATTGTGTG CTATGCAGCT CTTCTCACAC ATGCACGCCG TCATCAGCAC TGCCACCTGC
    ATGCGGCGCA GCTTCATCCA GAGCACCTTC AGCATCAACC CAGAAATCGT CTGTGACCCC
    TTATCCGACT ACAACGTATG GAGCATGCTT AAGCCTATAA ATACATCTGT GGGACTAGAA
    CCTGACGTCA GGGTTGTGGT TGCGGCCACA CGGCTGGATA GCCGGTCCTT TTTCTGGAAT
    GTGGCCCCAG GGGCTGAAAG TGCTGTAGCC TCCTTTGTCA CTCAGCTGGC TGCAGCTGAA
    GCTTTGCACA AGGCACCTGA TGTGACCACT CTATCCCGAA ATGTGATGTT TGTCTTCTTC
    CAGGGGGAAA CTTTTGACTA CATTGGCAGC TCACGGATGG TCTATGATAT GGAGAACGGC
    AAGTTTCCCG TGCGGCTCGA GAACATTGAC TCCTTCGTGG AGCTGGGACA GGTGGCCCTA
    AGAACTTCAC TAGATCTCTG GATGCACACA GATCCCATGT CTCAGAAAAA TGAGTCTGTG
    AAAAACCAGG TGGAGGATCT TCTGGCCACT CTGGAGAAGA GCGGTGCTGG TGTCCCTGAA
    GTTGTCCTGA GGAGACTGGC CCAGTCCCAG GCCCTTCCAC CTTCGTCTCT ACAACGATTT
    CTTCGGGCTC GAAACATCTC TGGCGTGGTC CTGGCTGACC ACTCTGGCTC CTTCCACAAT
    CGGTATTACC AGAGCATTTA TGACACGGCT GAGAACATTA ATGTGACCTA TCCTGAGTGG
    CAGAGCCCAG AAGAGGACCT CAACTTTGTG ACAGACACTG CCAAGGCACT GGCGAATGTG
    GCCACAGTGC TGGCGCGTGC ACTGTATGAG CTTGCAGGAG GAACCAACTT CAGCAGCTCC
    ATCCAGGCTG ATCCCCAGAC AGTTACACGT CTGCTCTATG GGTTCCTGGT TAGAGCTAAC
    AACTCATGGT TTCAGTCGAT CTTGAAACAT GACCTAAGGT CCTATTTGGA TGACAGGCCT
    CTTCAACACT ACATCGCCGT CTCCAGCCCT ACCAACACGA CTTACGTTGT GCAGTACGCC
    TTGGCAAACC TGACTGGCAA GGCGACCAAC CTCACCCGAG AGCAGTGCCA GGATCCAAGT
    AAAGTCCCAA ATGAGAGCAA GGATTTATAT GAATACTCGT GGGTACAAGG CCCTTGGAAT
    TCCAACAGGA CAGAGAGGCT CCCACAGTGT GTGCGCTCCA CAGTGCGACT GGCCAGGGCC
    TTGTCCCCTG CCTTTGAACT GAGTCAGTGG AGCTCCACAG AATACTCTAC GTGGGCGGAG
    AGCCGCTGGA AAGACATCCA AGCTCGGATA TTCCTAATTG CCAGCAAAGA GCTTGAGTTC
    ATCACGCTGA TCGTGGGCTT CAGCACCCTT GTCTTCTCCC TCATCGTCAC CTACTGCATC
    AATGCCAAAG CCGACGTCCT TTTCGTTGCT CCCCGAGAGC CAGGAGCTGT GTCTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497015.1
    CDS16..2115
    Translation

    Target ORF information:

    RefSeq Version XM_006496952.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus nicastrin (Ncstn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496952.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCCTCGTT CTCTGTTTAA AGCCCAGCCA CCCCATCAGC CATCAAAAGG GACAGGATTG 
    TGTGGGGGAA ACTCAGTGGA GAGGAAAATC TACATTCCCT TAAATAAAAC AGCTCCTTGT
    GTCCGCCTGC TCAACGCCAC TCATCAGATT GGCTGCCAGT CTTCAATTAG TGGGGATACA
    GGGGTTATCC ATGTAGTGGA GAAAGAAGAA GACCTGAAGT GGGTGTTGAC CGATGGCCCC
    AACCCCCCTT ACATGGTTCT GCTGGAGGGC AAGCTCTTCA CCAGAGATGT AATGGAGAAG
    CTGAAGGGAA CAACCAGTAG AATCGCTGGT CTTGCCGTGA CTCTAGCCAA GCCCAACTCA
    ACTTCAAGCT TCTCTCCTAG TGTGCAGTGC CCAAATGATG GGTTTGGTAT TTACTCCAAC
    TCCTACGGGC CAGAGTTTGC TCACTGCAAG AAAACACTGT GGAATGAACT GGGCAACGGC
    TTGGCTTATG AAGACTTTAG TTTCCCCATC TTTCTTCTTG AAGATGAGAA CGAAACCAAG
    GTCATCAAGC AGTGCTATCA AGATCACAAC CTGGGTCAGA ATGGCTCTGC ACCAAGCTTC
    CCATTGTGTG CTATGCAGCT CTTCTCACAC ATGCACGCCG TCATCAGCAC TGCCACCTGC
    ATGCGGCGCA GCTTCATCCA GAGCACCTTC AGCATCAACC CAGAAATCGT CTGTGACCCC
    TTATCCGACT ACAACGTATG GAGCATGCTT AAGCCTATAA ATACATCTGT GGGACTAGAA
    CCTGACGTCA GGGTTGTGGT TGCGGCCACA CGGCTGGATA GCCGGTCCTT TTTCTGGAAT
    GTGGCCCCAG GGGCTGAAAG TGCTGTAGCC TCCTTTGTCA CTCAGCTGGC TGCAGCTGAA
    GCTTTGCACA AGGCACCTGA TGTGACCACT CTATCCCGAA ATGTGATGTT TGTCTTCTTC
    CAGGGGGAAA CTTTTGACTA CATTGGCAGC TCACGGATGG TCTATGATAT GGAGAACGGC
    AAGTTTCCCG TGCGGCTCGA GAACATTGAC TCCTTCGTGG AGCTGGGACA GGTGGCCCTA
    AGAACTTCAC TAGATCTCTG GATGCACACA GATCCCATGT CTCAGAAAAA TGAGTCTGTG
    AAAAACCAGG TGGAGGATCT TCTGGCCACT CTGGAGAAGA GCGGTGCTGG TGTCCCTGAA
    GTTGTCCTGA GGAGACTGGC CCAGTCCCAG GCCCTTCCAC CTTCGTCTCT ACAACGATTT
    CTTCGGGCTC GAAACATCTC TGGCGTGGTC CTGGCTGACC ACTCTGGCTC CTTCCACAAT
    CGGTATTACC AGAGCATTTA TGACACGGCT GAGAACATTA ATGTGACCTA TCCTGAGTGG
    CAGAGCCCAG AAGAGGACCT CAACTTTGTG ACAGACACTG CCAAGGCACT GGCGAATGTG
    GCCACAGTGC TGGCGCGTGC ACTGTATGAG CTTGCAGGAG GAACCAACTT CAGCAGCTCC
    ATCCAGGCTG ATCCCCAGAC AGTTACACGT CTGCTCTATG GGTTCCTGGT TAGAGCTAAC
    AACTCATGGT TTCAGTCGAT CTTGAAACAT GACCTAAGGT CCTATTTGGA TGACAGGCCT
    CTTCAACACT ACATCGCCGT CTCCAGCCCT ACCAACACGA CTTACGTTGT GCAGTACGCC
    TTGGCAAACC TGACTGGCAA GGCGACCAAC CTCACCCGAG AGCAGTGCCA GGATCCAAGT
    AAAGTCCCAA ATGAGAGCAA GGATTTATAT GAATACTCGT GGGTACAAGG CCCTTGGAAT
    TCCAACAGGA CAGAGAGGCT CCCACAGTGT GTGCGCTCCA CAGTGCGACT GGCCAGGGCC
    TTGTCCCCTG CCTTTGAACT GAGTCAGTGG AGCTCCACAG AATACTCTAC GTGGGCGGAG
    AGCCGCTGGA AAGACATCCA AGCTCGGATA TTCCTAATTG CCAGCAAAGA GCTTGAGTTC
    ATCACGCTGA TCGTGGGCTT CAGCACCCTT GTCTTCTCCC TCATCGTCAC CTACTGCATC
    AATGCCAAAG CCGACGTCCT TTTCGTTGCT CCCCGAGAGC CAGGAGCTGT GTCTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28821
    Clone ID Related Accession (Same CDS sequence) XM_006496952.4 , XM_006496952.3
    Accession Version XM_006496952.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product nicastrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496952.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCCTCGTT CTCTGTTTAA AGCCCAGCCA CCCCATCAGC CATCAAAAGG GACAGGATTG 
    TGTGGGGGAA ACTCAGTGGA GAGGAAAATC TACATTCCCT TAAATAAAAC AGCTCCTTGT
    GTCCGCCTGC TCAACGCCAC TCATCAGATT GGCTGCCAGT CTTCAATTAG TGGGGATACA
    GGGGTTATCC ATGTAGTGGA GAAAGAAGAA GACCTGAAGT GGGTGTTGAC CGATGGCCCC
    AACCCCCCTT ACATGGTTCT GCTGGAGGGC AAGCTCTTCA CCAGAGATGT AATGGAGAAG
    CTGAAGGGAA CAACCAGTAG AATCGCTGGT CTTGCCGTGA CTCTAGCCAA GCCCAACTCA
    ACTTCAAGCT TCTCTCCTAG TGTGCAGTGC CCAAATGATG GGTTTGGTAT TTACTCCAAC
    TCCTACGGGC CAGAGTTTGC TCACTGCAAG AAAACACTGT GGAATGAACT GGGCAACGGC
    TTGGCTTATG AAGACTTTAG TTTCCCCATC TTTCTTCTTG AAGATGAGAA CGAAACCAAG
    GTCATCAAGC AGTGCTATCA AGATCACAAC CTGGGTCAGA ATGGCTCTGC ACCAAGCTTC
    CCATTGTGTG CTATGCAGCT CTTCTCACAC ATGCACGCCG TCATCAGCAC TGCCACCTGC
    ATGCGGCGCA GCTTCATCCA GAGCACCTTC AGCATCAACC CAGAAATCGT CTGTGACCCC
    TTATCCGACT ACAACGTATG GAGCATGCTT AAGCCTATAA ATACATCTGT GGGACTAGAA
    CCTGACGTCA GGGTTGTGGT TGCGGCCACA CGGCTGGATA GCCGGTCCTT TTTCTGGAAT
    GTGGCCCCAG GGGCTGAAAG TGCTGTAGCC TCCTTTGTCA CTCAGCTGGC TGCAGCTGAA
    GCTTTGCACA AGGCACCTGA TGTGACCACT CTATCCCGAA ATGTGATGTT TGTCTTCTTC
    CAGGGGGAAA CTTTTGACTA CATTGGCAGC TCACGGATGG TCTATGATAT GGAGAACGGC
    AAGTTTCCCG TGCGGCTCGA GAACATTGAC TCCTTCGTGG AGCTGGGACA GGTGGCCCTA
    AGAACTTCAC TAGATCTCTG GATGCACACA GATCCCATGT CTCAGAAAAA TGAGTCTGTG
    AAAAACCAGG TGGAGGATCT TCTGGCCACT CTGGAGAAGA GCGGTGCTGG TGTCCCTGAA
    GTTGTCCTGA GGAGACTGGC CCAGTCCCAG GCCCTTCCAC CTTCGTCTCT ACAACGATTT
    CTTCGGGCTC GAAACATCTC TGGCGTGGTC CTGGCTGACC ACTCTGGCTC CTTCCACAAT
    CGGTATTACC AGAGCATTTA TGACACGGCT GAGAACATTA ATGTGACCTA TCCTGAGTGG
    CAGAGCCCAG AAGAGGACCT CAACTTTGTG ACAGACACTG CCAAGGCACT GGCGAATGTG
    GCCACAGTGC TGGCGCGTGC ACTGTATGAG CTTGCAGGAG GAACCAACTT CAGCAGCTCC
    ATCCAGGCTG ATCCCCAGAC AGTTACACGT CTGCTCTATG GGTTCCTGGT TAGAGCTAAC
    AACTCATGGT TTCAGTCGAT CTTGAAACAT GACCTAAGGT CCTATTTGGA TGACAGGCCT
    CTTCAACACT ACATCGCCGT CTCCAGCCCT ACCAACACGA CTTACGTTGT GCAGTACGCC
    TTGGCAAACC TGACTGGCAA GGCGACCAAC CTCACCCGAG AGCAGTGCCA GGATCCAAGT
    AAAGTCCCAA ATGAGAGCAA GGATTTATAT GAATACTCGT GGGTACAAGG CCCTTGGAAT
    TCCAACAGGA CAGAGAGGCT CCCACAGTGT GTGCGCTCCA CAGTGCGACT GGCCAGGGCC
    TTGTCCCCTG CCTTTGAACT GAGTCAGTGG AGCTCCACAG AATACTCTAC GTGGGCGGAG
    AGCCGCTGGA AAGACATCCA AGCTCGGATA TTCCTAATTG CCAGCAAAGA GCTTGAGTTC
    ATCACGCTGA TCGTGGGCTT CAGCACCCTT GTCTTCTCCC TCATCGTCAC CTACTGCATC
    AATGCCAAAG CCGACGTCCT TTTCGTTGCT CCCCGAGAGC CAGGAGCTGT GTCTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497015.1
    CDS94..2193
    Translation

    Target ORF information:

    RefSeq Version XM_006496952.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus nicastrin (Ncstn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496952.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCCTCGTT CTCTGTTTAA AGCCCAGCCA CCCCATCAGC CATCAAAAGG GACAGGATTG 
    TGTGGGGGAA ACTCAGTGGA GAGGAAAATC TACATTCCCT TAAATAAAAC AGCTCCTTGT
    GTCCGCCTGC TCAACGCCAC TCATCAGATT GGCTGCCAGT CTTCAATTAG TGGGGATACA
    GGGGTTATCC ATGTAGTGGA GAAAGAAGAA GACCTGAAGT GGGTGTTGAC CGATGGCCCC
    AACCCCCCTT ACATGGTTCT GCTGGAGGGC AAGCTCTTCA CCAGAGATGT AATGGAGAAG
    CTGAAGGGAA CAACCAGTAG AATCGCTGGT CTTGCCGTGA CTCTAGCCAA GCCCAACTCA
    ACTTCAAGCT TCTCTCCTAG TGTGCAGTGC CCAAATGATG GGTTTGGTAT TTACTCCAAC
    TCCTACGGGC CAGAGTTTGC TCACTGCAAG AAAACACTGT GGAATGAACT GGGCAACGGC
    TTGGCTTATG AAGACTTTAG TTTCCCCATC TTTCTTCTTG AAGATGAGAA CGAAACCAAG
    GTCATCAAGC AGTGCTATCA AGATCACAAC CTGGGTCAGA ATGGCTCTGC ACCAAGCTTC
    CCATTGTGTG CTATGCAGCT CTTCTCACAC ATGCACGCCG TCATCAGCAC TGCCACCTGC
    ATGCGGCGCA GCTTCATCCA GAGCACCTTC AGCATCAACC CAGAAATCGT CTGTGACCCC
    TTATCCGACT ACAACGTATG GAGCATGCTT AAGCCTATAA ATACATCTGT GGGACTAGAA
    CCTGACGTCA GGGTTGTGGT TGCGGCCACA CGGCTGGATA GCCGGTCCTT TTTCTGGAAT
    GTGGCCCCAG GGGCTGAAAG TGCTGTAGCC TCCTTTGTCA CTCAGCTGGC TGCAGCTGAA
    GCTTTGCACA AGGCACCTGA TGTGACCACT CTATCCCGAA ATGTGATGTT TGTCTTCTTC
    CAGGGGGAAA CTTTTGACTA CATTGGCAGC TCACGGATGG TCTATGATAT GGAGAACGGC
    AAGTTTCCCG TGCGGCTCGA GAACATTGAC TCCTTCGTGG AGCTGGGACA GGTGGCCCTA
    AGAACTTCAC TAGATCTCTG GATGCACACA GATCCCATGT CTCAGAAAAA TGAGTCTGTG
    AAAAACCAGG TGGAGGATCT TCTGGCCACT CTGGAGAAGA GCGGTGCTGG TGTCCCTGAA
    GTTGTCCTGA GGAGACTGGC CCAGTCCCAG GCCCTTCCAC CTTCGTCTCT ACAACGATTT
    CTTCGGGCTC GAAACATCTC TGGCGTGGTC CTGGCTGACC ACTCTGGCTC CTTCCACAAT
    CGGTATTACC AGAGCATTTA TGACACGGCT GAGAACATTA ATGTGACCTA TCCTGAGTGG
    CAGAGCCCAG AAGAGGACCT CAACTTTGTG ACAGACACTG CCAAGGCACT GGCGAATGTG
    GCCACAGTGC TGGCGCGTGC ACTGTATGAG CTTGCAGGAG GAACCAACTT CAGCAGCTCC
    ATCCAGGCTG ATCCCCAGAC AGTTACACGT CTGCTCTATG GGTTCCTGGT TAGAGCTAAC
    AACTCATGGT TTCAGTCGAT CTTGAAACAT GACCTAAGGT CCTATTTGGA TGACAGGCCT
    CTTCAACACT ACATCGCCGT CTCCAGCCCT ACCAACACGA CTTACGTTGT GCAGTACGCC
    TTGGCAAACC TGACTGGCAA GGCGACCAAC CTCACCCGAG AGCAGTGCCA GGATCCAAGT
    AAAGTCCCAA ATGAGAGCAA GGATTTATAT GAATACTCGT GGGTACAAGG CCCTTGGAAT
    TCCAACAGGA CAGAGAGGCT CCCACAGTGT GTGCGCTCCA CAGTGCGACT GGCCAGGGCC
    TTGTCCCCTG CCTTTGAACT GAGTCAGTGG AGCTCCACAG AATACTCTAC GTGGGCGGAG
    AGCCGCTGGA AAGACATCCA AGCTCGGATA TTCCTAATTG CCAGCAAAGA GCTTGAGTTC
    ATCACGCTGA TCGTGGGCTT CAGCACCCTT GTCTTCTCCC TCATCGTCAC CTACTGCATC
    AATGCCAAAG CCGACGTCCT TTTCGTTGCT CCCCGAGAGC CAGGAGCTGT GTCTTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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