Ifi30 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Ifi30
Entrez Gene ID	65972
Full Name	interferon gamma inducible protein 30
Synonyms	GILT,IP30
General protein information	Preferred Names interferon gamma inducible protein 30 Names gamma-interferon-inducible lysosomal thiol reductase gamma-interferon-inducible protein IP-30 lysosomal thio reductase IP30 lysosomal thiol reductase IP30
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 B3.3

mRNA and Protein(s)

mRNA Protein Name

NM_023065.3 NP_075552.2 gamma-interferon-inducible lysosomal thiol reductase precursor
Pathways from BioSystems

mRNA	Protein	Name
NM_023065.3	NP_075552.2	gamma-interferon-inducible lysosomal thiol reductase precursor

Homologies

Mus musculus (house mouse)	Ifi30	NP_075552.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009747	XP_318831.4
Canis lupus familiaris (dog)	LOC476669	XP_533874.3
Pan troglodytes (chimpanzee)	IFI30	XP_001162304.2
Bos taurus (cattle)	IFI30	NP_001094721.1
Oryza sativa (rice)	Os03g0295800	NP_001049827.1
Arabidopsis thaliana (thale cress)	AT1G07080	NP_563779.1
Homo sapiens (human)	IFI30	NP_006323.2
	LOC719379	XP_001114116.2
Rattus norvegicus (Norway rat)	Ifi30	NP_001025197.1
Gallus gallus (chicken)	IFI30	XP_004948968.1
Drosophila melanogaster (fruit fly)	CG41378	NP_001163836.1
Xenopus tropicalis (tropical clawed frog)	ifi30	NP_001017196.1

Gene Ontology
Bibliography

Related articles in PubMed

An exhaustion-like phenotype constrains the activity of CD4+ T cells specific for a self and melanoma antigen.
Rausch MP, Hastings KT
PloS one10(4)e0123332(2015)

?-Interferon-inducible lysosomal thiol reductase (GILT) maintains phagosomal proteolysis in alternatively activated macrophages.
Balce DR, Allan ER, McKenna N, Yates RM
The Journal of biological chemistry289(46)31891-904(2014 Nov)

Gamma interferon-inducible lysosomal thioreductase (GILT) ablation renders mouse fibroblasts sensitive to dengue virus replication.
Teramoto T, Chiang HS, Takhampunya R, Manzano M, Padmanabhan R, Maric M
Virology441(2)146-51(2013 Jul)

MHC class II-restricted presentation of the major house dust mite allergen Der p 1 Is GILT-dependent: implications for allergic asthma.
West LC, Grotzke JE, Cresswell P
PloS one8(1)e51343(2013)

GILT expression in B cells diminishes cathepsin S steady-state protein expression and activity.
Phipps-Yonas H, Semik V, Hastings KT
European journal of immunology43(1)65-74(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GILT has a role in maintaining cysteine cathepsin proteolytic efficiency in phagosomes
Title: ?-Interferon-inducible lysosomal thiol reductase (GILT) maintains phagosomal proteolysis in alternatively activated macrophages.

These results show that wild-type mouse fibroblasts are relatively resistant and GILT(-/-)mouse fibroblasts are susceptible to Dengue virus 2 entry and replication.
Title: Gamma interferon-inducible lysosomal thioreductase (GILT) ablation renders mouse fibroblasts sensitive to dengue virus replication.

Data indicate that in the absence of Gamma-interferon-inducible lysosomal thiol reductase (GIL, CD4-positive T cell responses to major house dust mite allergen Der p 1 are significantly reduced.
Title: MHC class II-restricted presentation of the major house dust mite allergen Der p 1 Is GILT-dependent: implications for allergic asthma.

GILT has a critical role in regulating CD4(+) T-cell tolerance to an endogenous skin-restricted antigen relevant to controlling autoimmunity and generating effective immunotherapy for melanoma.
Title: GILT modulates CD4+ T-cell tolerance to the melanocyte differentiation antigen tyrosinase-related protein 1.

gene knock-outs increase oxidative stress and autophagy, which is regulated by altering glutathione synthesis and oxidation
Title: Lysosomal thiol reductase negatively regulates autophagy by altering glutathione synthesis and oxidation.

The following Ifi30 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ifi30 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00124	NM_023065.3 Latest version!	Mus musculus interferon gamma inducible protein 30 (Ifi30), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ifi30 ( NM_023065.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075552.2 Mus musculus interferon gamma inducible protein 30 (Ifi30), mRNA.

Price & Availability↑

CloneID	OMu00124
Clone ID Related Accession (Same CDS sequence)	NM_023065.3
Accession Version	NM_023065.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	gamma-interferon-inducible lysosomal thiol reductase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY774497.1, AF309649.1, BQ746455.1 and BE951500.1. On Feb 10, 2007 this sequence version replaced NM_023065.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054852.1, AK077725.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTGGT CTCCGATCCT ACCATTTTTG TCCCTTCTGC TTCTGCTGTT CCCACTCGAG 
GTTCCCAGAG CAGCCACTGC GTCCCTTTCG CAAGCATCCT CCGAAGGCAC AACCACCTGC 
AAGGCCCACG ATGTGTGCCT CTTGGGGCCA CGCCCATTGC CCCCTTCACC ACCTGTCAGA 
GTCAGCCTGT ATTATGAGTC CCTGTGTGGA GCTTGTCGCT ACTTCCTCGT CCGGGATTTG 
TTCCCAACCT GGCTGATGGT GATGGAAATC ATGAACATCA CCCTGGTGCC CTACGGGAAT 
GCACAGGAGA GAAATGTCAG CGGTACGTGG GAGTTCACGT GCCAGCACGG GGAGCTGGAG 
TGTAGACTGA ACATGGTGGA GGCCTGTCTG CTGGATAAGC TGGAAAAGGA GGCAGCGTTC 
CTAACCATCG TCTGTATGGA GGAGATGGAT GATATGGAGA AGAAACTGGG ACCGTGCCTG 
CAGGTGTATG CTCCTGAGGT GTCACCAGAG AGTATCATGG AGTGCGCCAC AGGAAAACGG 
GGCACACAGC TCATGCATGA GAATGCCCAG CTCACAGATG CCCTACACCC ACCCCACGAG 
TATGTGCCCT GGGTGCTGGT CAATGAGAAA CCTTTGAAGG ACCCCAGCGA GCTCCTGAGC 
ATAGTCTGTC AGCTGTACCA GGGAACGGAG AAGCCAGACA TCTGCTCCTC CATTGCCGAC 
TCCCCCAGGA AGGTCTGCTA TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075552.2
CDS	33..779
Translation	MSWSPILPFLSLLLLLFPLEVPRAATASLSQASSEGTTTCKAHDVCLLGPRPLPPSPPVRVSLYYESLCGACRYFLVRDLFPTWLMVMEIMNITLVPYGNAQERNVSGTWEFTCQHGELECRLNMVEACLLDKLEKEAAFLTIVCMEEMDDMEKKLGPCLQVYAPEVSPESIMECATGKRGTQLMHENAQLTDALHPPHEYVPWVLVNEKPLKDPSELLSIVCQLYQGTEKPDICSSIADSPRKVCYK

Target ORF information:

RefSeq Version	NM_023065.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus interferon gamma inducible protein 30 (Ifi30), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023065.3

ORF Insert Sequence:

ATGTCCTGGT CTCCGATCCT ACCATTTTTG TCCCTTCTGC TTCTGCTGTT CCCACTCGAG 
GTTCCCAGAG CAGCCACTGC GTCCCTTTCG CAAGCATCCT CCGAAGGCAC AACCACCTGC 
AAGGCCCACG ATGTGTGCCT CTTGGGGCCA CGCCCATTGC CCCCTTCACC ACCTGTCAGA 
GTCAGCCTGT ATTATGAGTC CCTGTGTGGA GCTTGTCGCT ACTTCCTCGT CCGGGATTTG 
TTCCCAACCT GGCTGATGGT GATGGAAATC ATGAACATCA CCCTGGTGCC CTACGGGAAT 
GCACAGGAGA GAAATGTCAG CGGTACGTGG GAGTTCACGT GCCAGCACGG GGAGCTGGAG 
TGTAGACTGA ACATGGTGGA GGCCTGTCTG CTGGATAAGC TGGAAAAGGA GGCAGCGTTC 
CTAACCATCG TCTGTATGGA GGAGATGGAT GATATGGAGA AGAAACTGGG ACCGTGCCTG 
CAGGTGTATG CTCCTGAGGT GTCACCAGAG AGTATCATGG AGTGCGCCAC AGGAAAACGG 
GGCACACAGC TCATGCATGA GAATGCCCAG CTCACAGATG CCCTACACCC ACCCCACGAG 
TATGTGCCCT GGGTGCTGGT CAATGAGAAA CCTTTGAAGG ACCCCAGCGA GCTCCTGAGC 
ATAGTCTGTC AGCTGTACCA GGGAACGGAG AAGCCAGACA TCTGCTCCTC CATTGCCGAC 
TCCCCCAGGA AGGTCTGCTA TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An exhaustion-like phenotype constrains the activity of CD4+ T cells specific for a self and melanoma antigen.
PloS one10(4)e0123332(2015)
Rausch MP,Hastings KT

?-Interferon-inducible lysosomal thiol reductase (GILT) maintains phagosomal proteolysis in alternatively activated macrophages.
The Journal of biological chemistry289(46)31891-904(2014 Nov)
Balce DR,Allan ER,McKenna N,Yates RM

Gamma interferon-inducible lysosomal thioreductase (GILT) ablation renders mouse fibroblasts sensitive to dengue virus replication.
Virology441(2)146-51(2013 Jul)
Teramoto T,Chiang HS,Takhampunya R,Manzano M,Padmanabhan R,Maric M

MHC class II-restricted presentation of the major house dust mite allergen Der p 1 Is GILT-dependent: implications for allergic asthma.
PloS one8(1)e51343(2013)
West LC,Grotzke JE,Cresswell P

GILT expression in B cells diminishes cathepsin S steady-state protein expression and activity.
European journal of immunology43(1)65-74(2013 Jan)
Phipps-Yonas H,Semik V,Hastings KT