Iscu cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Iscu
Entrez Gene ID	66383
Full Name	iron-sulfur cluster assembly enzyme
Synonyms	2310020H20Rik,AA407971,Nifu,Nifun
General protein information	Preferred Names iron-sulfur cluster assembly enzyme Names iron-sulfur cluster assembly enzyme ISCU, mitochondrial ISCU2 IscU iron-sulfur cluster scaffold homolog NifU-like N-terminal domain containing nifU-like N-terminal domain-containing protein nifU-like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
NM_025526.4	NP_079802.1	iron-sulfur cluster assembly enzyme ISCU, mitochondrial precursor
NM_001363317.1	NP_001350246.1	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 2
XM_030254754.1	XP_030110614.1	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform X1
XM_030254755.1	XP_030110615.1	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform X2
NM_025526.5	NP_079802.1	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ISCU	XP_509343.2
Canis lupus familiaris (dog)	LOC477527	XP_534722.1
Mus musculus (house mouse)	Iscu	NP_079802.1
Drosophila melanogaster (fruit fly)	CG9836	NP_649840.1
Oryza sativa (rice)	Os01g0662600	NP_001043783.1
Oryza sativa (rice)	Os05g0568100	NP_001056355.1
Xenopus tropicalis (tropical clawed frog)	iscu	NP_989088.1
Bos taurus (cattle)	ISCU	NP_001069151.1
Danio rerio (zebrafish)	iscub	NP_001017800.2
Caenorhabditis elegans (roundworm)	Y45F10D.4	NP_502658.1
Saccharomyces cerevisiae (baker's yeast)	ISU1	NP_015190.1
Danio rerio (zebrafish)	si:ch211-191d15.2	NP_001003632.1
Saccharomyces cerevisiae (baker's yeast)	ISU2	NP_014869.3
Eremothecium gossypii	AGOS_ACR112C	NP_983514.1
Neurospora crassa	NCU05778	XP_960038.1
Homo sapiens (human)	ISCU	NP_998760.1
Rattus norvegicus (Norway rat)	Iscu	NP_001099406.1
Gallus gallus (chicken)	ISCU	XP_003642230.2
Arabidopsis thaliana (thale cress)	ISU1	NP_193953.1
Arabidopsis thaliana (thale cress)	ISU2	NP_186751.1
	ISCU	XP_001103180.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005813	XP_315834.2
Kluyveromyces lactis	KLLA0D07161g	XP_453380.1
Schizosaccharomyces pombe (fission yeast)	isu1	NP_592967.1
Arabidopsis thaliana (thale cress)	ISU3	NP_192317.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of iron homeostasis by the p53-ISCU pathway.
Funauchi Y, Tanikawa C, Yi Lo PH, Mori J, Daigo Y, Takano A, Miyagi Y, Okawa A, Nakamura Y, Matsuda K
Scientific reports516497(2015 Nov)

The role of zinc in the stability of the marginally stable IscU scaffold protein.
Iannuzzi C, Adrover M, Puglisi R, Yan R, Temussi PA, Pastore A
Protein science : a publication of the Protein Society23(9)1208-19(2014 Sep)

Mammalian target of rapamycin complex 1 (mTORC1)-mediated phosphorylation stabilizes ISCU protein: implications for iron metabolism.
La P, Yang G, Dennery PA
The Journal of biological chemistry288(18)12901-9(2013 May)

Tissue-specific splicing of ISCU results in a skeletal muscle phenotype in myopathy with lactic acidosis, while complete loss of ISCU results in early embryonic death in mice.
Nordin A, Larsson E, Thornell LE, Holmberg M
Human genetics129(4)371-8(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ISCU expression was decreased in the majority of human liver cancer tissues, and its reduced expression was significantly associated with p53 mutation.
Title: Regulation of iron homeostasis by the p53-ISCU pathway.

IscU is a marginally stable protein at low ionic strength to the point that undergoes cold denaturation at around -8 degrees C with a corresponding dramatic decrease of enthalpy, which is consistent with the fluxional nature of the protein.
Title: The role of zinc in the stability of the marginally stable IscU scaffold protein.

mTORC1 associates with ISCU and phosphorylates ISCU at serine 14. This phosphorylation stabilized ISCU protein.
Title: Mammalian target of rapamycin complex 1 (mTORC1)-mediated phosphorylation stabilizes ISCU protein: implications for iron metabolism.

Data show that complete loss of ISCU results in early embryonic death and confirm a fundamental role for ISCU in mammals.
Title: Tissue-specific splicing of ISCU results in a skeletal muscle phenotype in myopathy with lactic acidosis, while complete loss of ISCU results in early embryonic death in mice.

Results identify the iron-sulfur cluster assembly proteins (ISCU1/2) as direct targets for repression by the hypoxia-induced microRNA-210.
Title: MicroRNA-210 controls mitochondrial metabolism during hypoxia by repressing the iron-sulfur cluster assembly proteins ISCU1/2.

The following Iscu gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Iscu cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23073	NM_025526.5 Latest version!	Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OMu84275	NM_001363317.1 Latest version!	Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu88994	XM_030254754.1 Latest version!	Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu88995	XM_030254755.1 Latest version!	Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu23073	NM_025526.4	Mus musculus iron-sulfur cluster assembly enzyme (Iscu), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Iscu ( NM_025526.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079802.1 Mus musculus iron-sulfur cluster assembly enzyme (Iscu), mRNA.

Price & Availability↑

CloneID	OMu23073
Clone ID Related Accession (Same CDS sequence)	NM_025526.4 , NM_025526.5
Accession Version	NM_025526.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Mus musculus(house mouse)
Product	iron-sulfur cluster assembly enzyme ISCU, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC132939.23. On Oct 19, 2007 this sequence version replaced NM_025526.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK009021.1, AK083247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCGGCGG CCACGGGAGC TGGCCGTCTG AGGCGGGCGG CGTCGGCGCT GCTGCTGCGG 
AGCCCGCGCC TGCCCGCCCG GGAGCTGTCG GCTCCGGCCA GGCTCTACCA CAAGAAGGTT 
GTGGATCATT ATGAAAACCC TCGGAACGTG GGATCCCTTG ACAAGACATC TAAAAATGTT 
GGAACCGGAT TGGTGGGGGC TCCGGCATGT GGTGACGTCA TGAAACTGCA GATCCAGGTG 
GATGAAAAGG GGAAGATTGT GGACGCCAGA TTCAAAACAT TTGGCTGCGG CTCCGCCATT 
GCCTCCAGCT CCTTAGCCAC AGAGTGGGTA AAGGGGAAAA CGGTGGAGGA AGCCCTGACC 
ATCAAAAACA CCGACATCGC CAAGGAGCTC TGCCTGCCGC CTGTGAAACT GCACTGCTCC 
ATGCTGGCAG AAGACGCCAT CAAGGCCGCC CTGGCTGACT ACAAACTGAA GCAAGAGTCC 
AAGAAGGAGG AGCCAGAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079802.1
CDS	17..523
Translation	MAAATGAGRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQESKKEEPEKQ

Target ORF information:

RefSeq Version	NM_025526.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus iron-sulfur cluster assembly enzyme (Iscu), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025526.4

ORF Insert Sequence:

ATGGCGGCGG CCACGGGAGC TGGCCGTCTG AGGCGGGCGG CGTCGGCGCT GCTGCTGCGG 
AGCCCGCGCC TGCCCGCCCG GGAGCTGTCG GCTCCGGCCA GGCTCTACCA CAAGAAGGTT 
GTGGATCATT ATGAAAACCC TCGGAACGTG GGATCCCTTG ACAAGACATC TAAAAATGTT 
GGAACCGGAT TGGTGGGGGC TCCGGCATGT GGTGACGTCA TGAAACTGCA GATCCAGGTG 
GATGAAAAGG GGAAGATTGT GGACGCCAGA TTCAAAACAT TTGGCTGCGG CTCCGCCATT 
GCCTCCAGCT CCTTAGCCAC AGAGTGGGTA AAGGGGAAAA CGGTGGAGGA AGCCCTGACC 
ATCAAAAACA CCGACATCGC CAAGGAGCTC TGCCTGCCGC CTGTGAAACT GCACTGCTCC 
ATGCTGGCAG AAGACGCCAT CAAGGCCGCC CTGGCTGACT ACAAACTGAA GCAAGAGTCC 
AAGAAGGAGG AGCCAGAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Iscu ( NM_025526.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079802.1 Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23073
Clone ID Related Accession (Same CDS sequence)	NM_025526.4 , NM_025526.5
Accession Version	NM_025526.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132939.23. On May 17, 2018 this sequence version replaced NM_025526.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: CK791593.1, AK039834.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CCACGGGAGC TGGCCGTCTG AGGCGGGCGG CGTCGGCGCT GCTGCTGCGG 
AGCCCGCGCC TGCCCGCCCG GGAGCTGTCG GCTCCGGCCA GGCTCTACCA CAAGAAGGTT 
GTGGATCATT ATGAAAACCC TCGGAACGTG GGATCCCTTG ACAAGACATC TAAAAATGTT 
GGAACCGGAT TGGTGGGGGC TCCGGCATGT GGTGACGTCA TGAAACTGCA GATCCAGGTG 
GATGAAAAGG GGAAGATTGT GGACGCCAGA TTCAAAACAT TTGGCTGCGG CTCCGCCATT 
GCCTCCAGCT CCTTAGCCAC AGAGTGGGTA AAGGGGAAAA CGGTGGAGGA AGCCCTGACC 
ATCAAAAACA CCGACATCGC CAAGGAGCTC TGCCTGCCGC CTGTGAAACT GCACTGCTCC 
ATGCTGGCAG AAGACGCCAT CAAGGCCGCC CTGGCTGACT ACAAACTGAA GCAAGAGTCC 
AAGAAGGAGG AGCCAGAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079802.1
CDS	28..534
Translation	MAAATGAGRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQESKKEEPEKQ

Target ORF information:

RefSeq Version	NM_025526.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025526.5

ORF Insert Sequence:

ATGGCGGCGG CCACGGGAGC TGGCCGTCTG AGGCGGGCGG CGTCGGCGCT GCTGCTGCGG 
AGCCCGCGCC TGCCCGCCCG GGAGCTGTCG GCTCCGGCCA GGCTCTACCA CAAGAAGGTT 
GTGGATCATT ATGAAAACCC TCGGAACGTG GGATCCCTTG ACAAGACATC TAAAAATGTT 
GGAACCGGAT TGGTGGGGGC TCCGGCATGT GGTGACGTCA TGAAACTGCA GATCCAGGTG 
GATGAAAAGG GGAAGATTGT GGACGCCAGA TTCAAAACAT TTGGCTGCGG CTCCGCCATT 
GCCTCCAGCT CCTTAGCCAC AGAGTGGGTA AAGGGGAAAA CGGTGGAGGA AGCCCTGACC 
ATCAAAAACA CCGACATCGC CAAGGAGCTC TGCCTGCCGC CTGTGAAACT GCACTGCTCC 
ATGCTGGCAG AAGACGCCAT CAAGGCCGCC CTGGCTGACT ACAAACTGAA GCAAGAGTCC 
AAGAAGGAGG AGCCAGAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Iscu ( NM_001363317.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350246.1 Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu84275
Clone ID Related Accession (Same CDS sequence)	NM_001363317.1
Accession Version	NM_001363317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132939.23, AI851358.1 and AK083247.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AI851358.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGGC GAGGGGGTGC GCGAACCATC GAGGGGACCT GCCGCCGTTG CGCAGATCAC 
CAGCACCGAG GAGCCCCGCG GCCGCCTCTG CCCCGCCGCG GCCGTCACTC CGAGTGTCCC 
AAGGCTGACA GCCCAGAAGG GAGGGTCCGG GTTGTGGATC ATTATGAAAA CCCTCGGAAC 
GTGGGATCCC TTGACAAGAC ATCTAAAAAT GTTGGAACCG GATTGGTGGG GGCTCCGGCA 
TGTGGTGACG TCATGAAACT GCAGATCCAG GTGGATGAAA AGGGGAAGAT TGTGGACGCC 
AGATTCAAAA CATTTGGCTG CGGCTCCGCC ATTGCCTCCA GCTCCTTAGC CACAGAGTGG 
GTAAAGGGGA AAACGGTGGA GGAAGCCCTG ACCATCAAAA ACACCGACAT CGCCAAGGAG 
CTCTGCCTGC CGCCTGTGAA ACTGCACTGC TCCATGCTGG CAGAAGACGC CATCAAGGCC 
GCCCTGGCTG ACTACAAACT GAAGCAAGAG TCCAAGAAGG AGGAGCCAGA GAAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350246.1
CDS	147..686
Translation	MGGRGGARTIEGTCRRCADHQHRGAPRPPLPRRGRHSECPKADSPEGRVRVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQESKKEEPEKQ

Target ORF information:

RefSeq Version	NM_001363317.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363317.1

ORF Insert Sequence:

ATGGGCGGGC GAGGGGGTGC GCGAACCATC GAGGGGACCT GCCGCCGTTG CGCAGATCAC 
CAGCACCGAG GAGCCCCGCG GCCGCCTCTG CCCCGCCGCG GCCGTCACTC CGAGTGTCCC 
AAGGCTGACA GCCCAGAAGG GAGGGTCCGG GTTGTGGATC ATTATGAAAA CCCTCGGAAC 
GTGGGATCCC TTGACAAGAC ATCTAAAAAT GTTGGAACCG GATTGGTGGG GGCTCCGGCA 
TGTGGTGACG TCATGAAACT GCAGATCCAG GTGGATGAAA AGGGGAAGAT TGTGGACGCC 
AGATTCAAAA CATTTGGCTG CGGCTCCGCC ATTGCCTCCA GCTCCTTAGC CACAGAGTGG 
GTAAAGGGGA AAACGGTGGA GGAAGCCCTG ACCATCAAAA ACACCGACAT CGCCAAGGAG 
CTCTGCCTGC CGCCTGTGAA ACTGCACTGC TCCATGCTGG CAGAAGACGC CATCAAGGCC 
GCCCTGGCTG ACTACAAACT GAAGCAAGAG TCCAAGAAGG AGGAGCCAGA GAAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Iscu ( XM_030254754.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110614.1 Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu88994
Clone ID Related Accession (Same CDS sequence)	XM_030254754.1
Accession Version	XM_030254754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGATC GAATCATAAA CACGCCTGAA ACCGAGCATC CCCACGCCCA TCGCAGCACC 
AGACAACCTT GCACGCCGCT TCGGACCTTC CCGCTCTGCG CCTGGCCACG CCCCTTCCGA 
GGGCGCGGTC AGAGGGGGCG TAGCCGGGCC GAGACGCCAG CGCAAGCGCC GAGCTGCGGT 
GACGTCGAAC GGGCGCGCCA TCCCTCCGGC GTCGCCGAGG TCGACGCAGC TCAAGCCGGC 
AGGATGGCGG CGGCCACGGG AGCTGGCCGT CTGAGGCGGG CGGCGTCGGC GCTGCTGCTG 
CGGAGCCCGC GCCTGCCCGC CCGGGAGCTG TCGGCTCCGG CCAGGCTCTA CCACAAGAAG 
GTTGTGGATC ATTATGAAAA CCCTCGGAAC GTGGGATCCC TTGACAAGAC ATCTAAAAAT 
GTTGGAACCG GATTGGTGGG GGCTCCGGCA TGTGGTGACG TCATGAAACT GCAGATCCAG 
GTGGATGAAA AGGGGAAGAT TGTGGACGCC AGATTCAAAA CATTTGGCTG CGGCTCCGCC 
ATTGCCTCCA GCTCCTTAGC CACAGAGTGG GTAAAGGGGA AAACGGTGGA GGAAGCCCTG 
ACCATCAAAA ACACCGACAT CGCCAAGGAG CTCTGCCTGC CGCCTGTGAA ACTGCACTGC 
TCCAGTGAGT CTGCCCCACC TGCCAGGCAG CTTGGATCTG TTCAGAGTCT GCTGTTCCCA 
CCCCACGGTG GCCGGGCAGC TGAGACAGGC AGCTCTTCCC CCACCCTGCG CTTCCCCAGT 
CTGCCTTTGC TGTCTCTGAG TGTGTCTGCA GTGCCTGAGG CCACAGTGCA CTACCTACCA 
CACTGCCCCA CGAGAGCCTT CCCACACTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110614.1
CDS	168..1040
Translation	MHDRIINTPETEHPHAHRSTRQPCTPLRTFPLCAWPRPFRGRGQRGRSRAETPAQAPSCGDVERARHPSGVAEVDAAQAGRMAAATGAGRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSSESAPPARQLGSVQSLLFPPHGGRAAETGSSSPTLRFPSLPLLSLSVSAVPEATVHYLPHCPTRAFPHC

Target ORF information:

RefSeq Version	XM_030254754.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254754.1

ORF Insert Sequence:

ATGCATGATC GAATCATAAA CACGCCTGAA ACCGAGCATC CCCACGCCCA TCGCAGCACC 
AGACAACCTT GCACGCCGCT TCGGACCTTC CCGCTCTGCG CCTGGCCACG CCCCTTCCGA 
GGGCGCGGTC AGAGGGGGCG TAGCCGGGCC GAGACGCCAG CGCAAGCGCC GAGCTGCGGT 
GACGTCGAAC GGGCGCGCCA TCCCTCCGGC GTCGCCGAGG TCGACGCAGC TCAAGCCGGC 
AGGATGGCGG CGGCCACGGG AGCTGGCCGT CTGAGGCGGG CGGCGTCGGC GCTGCTGCTG 
CGGAGCCCGC GCCTGCCCGC CCGGGAGCTG TCGGCTCCGG CCAGGCTCTA CCACAAGAAG 
GTTGTGGATC ATTATGAAAA CCCTCGGAAC GTGGGATCCC TTGACAAGAC ATCTAAAAAT 
GTTGGAACCG GATTGGTGGG GGCTCCGGCA TGTGGTGACG TCATGAAACT GCAGATCCAG 
GTGGATGAAA AGGGGAAGAT TGTGGACGCC AGATTCAAAA CATTTGGCTG CGGCTCCGCC 
ATTGCCTCCA GCTCCTTAGC CACAGAGTGG GTAAAGGGGA AAACGGTGGA GGAAGCCCTG 
ACCATCAAAA ACACCGACAT CGCCAAGGAG CTCTGCCTGC CGCCTGTGAA ACTGCACTGC 
TCCAGTGAGT CTGCCCCACC TGCCAGGCAG CTTGGATCTG TTCAGAGTCT GCTGTTCCCA 
CCCCACGGTG GCCGGGCAGC TGAGACAGGC AGCTCTTCCC CCACCCTGCG CTTCCCCAGT 
CTGCCTTTGC TGTCTCTGAG TGTGTCTGCA GTGCCTGAGG CCACAGTGCA CTACCTACCA 
CACTGCCCCA CGAGAGCCTT CCCACACTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Iscu ( XM_030254755.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110615.1 Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu88995
Clone ID Related Accession (Same CDS sequence)	XM_030254755.1
Accession Version	XM_030254755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGGC GAGGGGGTGC GCGAACCATC GAGGGGACCT GCCGCCGTTG CGCAGATCAC 
CAGCACCGAG GAGCCCCGCG GCCGCCTCTG CCCCGCCGCG GCCGTCACTC CGAGTGTCCC 
AAGGCTGACA GCCCAGAAGG GAGGGTCCGG GTTGTGGATC ATTATGAAAA CCCTCGGAAC 
GTGGGATCCC TTGACAAGAC ATCTAAAAAT GTTGGAACCG GATTGGTGGG GGCTCCGGCA 
TGTGGTGACG TCATGAAACT GCAGATCCAG GTGGATGAAA AGGGGAAGAT TGTGGACGCC 
AGATTCAAAA CATTTGGCTG CGGCTCCGCC ATTGCCTCCA GCTCCTTAGC CACAGAGTGG 
GTAAAGGGGA AAACGGTGGA GGAAGCCCTG ACCATCAAAA ACACCGACAT CGCCAAGGAG 
CTCTGCCTGC CGCCTGTGAA ACTGCACTGC TCCAGTGAGT CTGCCCCACC TGCCAGGCAG 
CTTGGATCTG TTCAGAGTCT GCTGTTCCCA CCCCACGGTG GCCGGGCAGC TGAGACAGGC 
AGCTCTTCCC CCACCCTGCG CTTCCCCAGT CTGCCTTTGC TGTCTCTGAG TGTGTCTGCA 
GTGCCTGAGG CCACAGTGCA CTACCTACCA CACTGCCCCA CGAGAGCCTT CCCACACTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110615.1
CDS	177..839
Translation	MGGRGGARTIEGTCRRCADHQHRGAPRPPLPRRGRHSECPKADSPEGRVRVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSSESAPPARQLGSVQSLLFPPHGGRAAETGSSSPTLRFPSLPLLSLSVSAVPEATVHYLPHCPTRAFPHC

Target ORF information:

RefSeq Version	XM_030254755.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254755.1

ORF Insert Sequence:

ATGGGCGGGC GAGGGGGTGC GCGAACCATC GAGGGGACCT GCCGCCGTTG CGCAGATCAC 
CAGCACCGAG GAGCCCCGCG GCCGCCTCTG CCCCGCCGCG GCCGTCACTC CGAGTGTCCC 
AAGGCTGACA GCCCAGAAGG GAGGGTCCGG GTTGTGGATC ATTATGAAAA CCCTCGGAAC 
GTGGGATCCC TTGACAAGAC ATCTAAAAAT GTTGGAACCG GATTGGTGGG GGCTCCGGCA 
TGTGGTGACG TCATGAAACT GCAGATCCAG GTGGATGAAA AGGGGAAGAT TGTGGACGCC 
AGATTCAAAA CATTTGGCTG CGGCTCCGCC ATTGCCTCCA GCTCCTTAGC CACAGAGTGG 
GTAAAGGGGA AAACGGTGGA GGAAGCCCTG ACCATCAAAA ACACCGACAT CGCCAAGGAG 
CTCTGCCTGC CGCCTGTGAA ACTGCACTGC TCCAGTGAGT CTGCCCCACC TGCCAGGCAG 
CTTGGATCTG TTCAGAGTCT GCTGTTCCCA CCCCACGGTG GCCGGGCAGC TGAGACAGGC 
AGCTCTTCCC CCACCCTGCG CTTCCCCAGT CTGCCTTTGC TGTCTCTGAG TGTGTCTGCA 
GTGCCTGAGG CCACAGTGCA CTACCTACCA CACTGCCCCA CGAGAGCCTT CCCACACTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of iron homeostasis by the p53-ISCU pathway.
Scientific reports516497(2015 Nov)
Funauchi Y,Tanikawa C,Yi Lo PH,Mori J,Daigo Y,Takano A,Miyagi Y,Okawa A,Nakamura Y,Matsuda K

The role of zinc in the stability of the marginally stable IscU scaffold protein.
Protein science : a publication of the Protein Society23(9)1208-19(2014 Sep)
Iannuzzi C,Adrover M,Puglisi R,Yan R,Temussi PA,Pastore A

Mammalian target of rapamycin complex 1 (mTORC1)-mediated phosphorylation stabilizes ISCU protein: implications for iron metabolism.
The Journal of biological chemistry288(18)12901-9(2013 May)
La P,Yang G,Dennery PA

Tissue-specific splicing of ISCU results in a skeletal muscle phenotype in myopathy with lactic acidosis, while complete loss of ISCU results in early embryonic death in mice.
Human genetics129(4)371-8(2011 Apr)
Nordin A,Larsson E,Thornell LE,Holmberg M

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Iscu cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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