Med28 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Med28
Entrez Gene ID	66999
Full Name	mediator complex subunit 28
Synonyms	1500003D12Rik,AI451633,AU045690,Eg1,FKSG20,magicin
General protein information	Preferred Names mediator complex subunit 28 Names mediator of RNA polymerase II transcription subunit 28 endothelial-derived mediator of RNA polymerase II transcription, subunit 28 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B3

mRNA and Protein(s)

mRNA Protein Name

NM_025895.4 NP_080171.1 mediator of RNA polymerase II transcription subunit 28
Pathways from BioSystems

mRNA	Protein	Name
NM_025895.4	NP_080171.1	mediator of RNA polymerase II transcription subunit 28

Homologies

Bos taurus (cattle)	MED28	NP_001033627.1
Drosophila melanogaster (fruit fly)	MED28	NP_651397.1
Xenopus tropicalis (tropical clawed frog)	med28	NP_001096476.1
	LOC722863	XP_001118952.2
Mus musculus (house mouse)	Med28	NP_080171.1
Homo sapiens (human)	MED28	NP_079481.2
Canis lupus familiaris (dog)	MED28	XP_850777.1
Rattus norvegicus (Norway rat)	Med28	NP_001100687.1
Gallus gallus (chicken)	MED28	XP_003641296.2
Danio rerio (zebrafish)	med28	NP_001007771.1
Pan troglodytes (chimpanzee)	MED28	XP_003950342.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Mediator Subunit Med28 Is Essential for Mouse Peri-Implantation Development and Pluripotency.
Li L, Walsh RM, Wagh V, James MF, Beauchamp RL, Chang YS, Gusella JF, Hochedlinger K, Ramesh V
PloS one10(10)e0140192(2015)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Mediator and cohesin connect gene expression and chromatin architecture.
Kagey MH, Newman JJ, Bilodeau S, Zhan Y, Orlando DA, van Berkum NL, Ebmeier CC, Goossens J, Rahl PB, Levine SS, Taatjes DJ, Dekker J, Young RA
Nature467(7314)430-5(2010 Sep)

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
Krebs AR, Demmers J, Karmodiya K, Chang NC, Chang AC, Tora L
EMBO reports11(7)541-7(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings document the essential role of Med28 in the developing embryo as well as in acquisition and maintenance of pluripotency during reprogramming.
Title: Mediator Subunit Med28 Is Essential for Mouse Peri-Implantation Development and Pluripotency.

EG-1 has a role in breast tumor growth
Title: Targeted inhibition of EG-1 blocks breast tumor growth.

The following Med28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01240	NM_025895.4 Latest version!	Mus musculus mediator complex subunit 28 (Med28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Med28 ( NM_025895.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080171.1 Mus musculus mediator complex subunit 28 (Med28), mRNA.

Price & Availability↑

CloneID	OMu01240
Clone ID Related Accession (Same CDS sequence)	NM_025895.4
Accession Version	NM_025895.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 28
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV653139.1 and AC092711.14. On May 7, 2009 this sequence version replaced NM_025895.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DV653139.1, AK154249.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGT CCCTGGGAGG GATGTTCACT GGGCAGCCTC CAGGTCCCCC GCCGCCGCCG 
CCCGGGCTCC CAGGCCAGGC GTCGCTTCTG CAAGCAGCCC CTGGGGCCCC GAGACCATCT 
AACAGCACTT TGGTGGACGA GTTGGAGTCA TCCTTCGAGG CTTGCTTTGC TTCCCTTGTG 
AGTCAGGATT ATGTCAATGG GACTGATCAG GAAGAAATTC GAACTGGTGT TGATCAGTGT 
ATCCAGAAGT TTTTGGACAT TGCAAGACAG ACAGAATGTT TTTTCCTACA AAAAAGGTTG 
CAGTTATCTG TCCAGAAACC AGATCAAGTT ATCAAAGAGG ATGTCTCGGA ACTAAGGAGT 
GAACTGCAGC GGAAGGATGC GCTGGTCCAG AAGCACTTGA CAAAGCTGAG GCATTGGCAG 
CAGGTGCTGG AGGACATCAA TGTGCAGCAC AAGAAGCCGG CTGACATGCC TCAGGGATCC 
TTGGCCTTCC TCGAGCAGGC GTCTGCCAAC ATTCCTGCAC CTCTGAAGCA GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080171.1
CDS	98..634
Translation	MAASLGGMFTGQPPGPPPPPPGLPGQASLLQAAPGAPRPSNSTLVDELESSFEACFASLVSQDYVNGTDQEEIRTGVDQCIQKFLDIARQTECFFLQKRLQLSVQKPDQVIKEDVSELRSELQRKDALVQKHLTKLRHWQQVLEDINVQHKKPADMPQGSLAFLEQASANIPAPLKQT

Target ORF information:

RefSeq Version	NM_025895.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 28 (Med28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025895.4

ORF Insert Sequence:

ATGGCGGCGT CCCTGGGAGG GATGTTCACT GGGCAGCCTC CAGGTCCCCC GCCGCCGCCG 
CCCGGGCTCC CAGGCCAGGC GTCGCTTCTG CAAGCAGCCC CTGGGGCCCC GAGACCATCT 
AACAGCACTT TGGTGGACGA GTTGGAGTCA TCCTTCGAGG CTTGCTTTGC TTCCCTTGTG 
AGTCAGGATT ATGTCAATGG GACTGATCAG GAAGAAATTC GAACTGGTGT TGATCAGTGT 
ATCCAGAAGT TTTTGGACAT TGCAAGACAG ACAGAATGTT TTTTCCTACA AAAAAGGTTG 
CAGTTATCTG TCCAGAAACC AGATCAAGTT ATCAAAGAGG ATGTCTCGGA ACTAAGGAGT 
GAACTGCAGC GGAAGGATGC GCTGGTCCAG AAGCACTTGA CAAAGCTGAG GCATTGGCAG 
CAGGTGCTGG AGGACATCAA TGTGCAGCAC AAGAAGCCGG CTGACATGCC TCAGGGATCC 
TTGGCCTTCC TCGAGCAGGC GTCTGCCAAC ATTCCTGCAC CTCTGAAGCA GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Mediator Subunit Med28 Is Essential for Mouse Peri-Implantation Development and Pluripotency.
PloS one10(10)e0140192(2015)
Li L,Walsh RM,Wagh V,James MF,Beauchamp RL,Chang YS,Gusella JF,Hochedlinger K,Ramesh V

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Mediator and cohesin connect gene expression and chromatin architecture.
Nature467(7314)430-5(2010 Sep)
Kagey MH,Newman JJ,Bilodeau S,Zhan Y,Orlando DA,van Berkum NL,Ebmeier CC,Goossens J,Rahl PB,Levine SS,Taatjes DJ,Dekker J,Young RA

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
EMBO reports11(7)541-7(2010 Jul)
Krebs AR,Demmers J,Karmodiya K,Chang NC,Chang AC,Tora L

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Med28 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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