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Tmigd3 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Tmigd3
Entrez Gene ID 69296
Full Name transmembrane and immunoglobulin domain containing 3
Synonyms 1700001d09rik,4930578J19Rik,Tg(H2-K-CALR*)del52Shmd
General protein information
Preferred Names

transmembrane and immunoglobulin domain containing 3

Names

transmembrane domain-containing protein TMIGD3
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

3

3 F2.2|3
Summary This gene encodes a transmembrane and immunoglobulin domain-containing protein. Alternative splicing results in multiple transcript variants, one of which shares its 3' terminal exon with that of the overlapping adenosine A3 receptor gene (GeneID:11542). [provided by RefSeq, Nov 2014].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001174169.2 NP_001167640.1 transmembrane domain-containing protein TMIGD3 isoform 3
NM_027025.4 NP_081301.2 transmembrane domain-containing protein TMIGD3 isoform 2 precursor

Pathways from BioSystems


Gene Ontology


Bibliography

Related articles in PubMed

Calreticulin mutant mice develop essential thrombocythemia that is ameliorated by the JAK inhibitor ruxolitinib.
Shide K, Kameda T, Yamaji T, Sekine M, Inada N, Kamiunten A, Akizuki K, Nakamura K, Hidaka T, Kubuki Y, Shimoda H, Kitanaka A, Honda A, Sawaguchi A, Abe H, Miike T, Iwakiri H, Tahara Y, Sueta M, Hasuike S, Yamamoto S, Nagata K, Shimoda K
Leukemia31(5)1136-1144(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tmigd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmigd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu19804 NM_001174169.2
Latest version!		 Mus musculus transmembrane and immunoglobulin domain containing 3 (Tmigd3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OMu19830 NM_027025.4
Latest version!		 Mus musculus transmembrane and immunoglobulin domain containing 3 (Tmigd3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu19804
Clone ID Related Accession (Same CDS sequence) NM_001174169.2
Accession Version NM_001174169.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 522bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-23
Organism Mus musculus(house mouse)
Product transmembrane domain-containing protein TMIGD3 isoform 3
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC100416.1 and AC121847.2. On Sep 24, 2013 this sequence version replaced NM_001174169.1. Transcript Variant: This variant (3) includes the same 5' terminal exon but lacks the remaining exons and instead includes an alternate 3' terminal exon, compared to variant 2. The 3' terminal exon is shared with that of the adenosine A3 receptor gene. The encoded isoform (3), which is shorter and distinct compared to isoform 2, represents the C-terminal region of the adenosine A3 receptor. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGTTTGGCT GGAATAGAAA AGCAACCTTA GCGAGCTCTC AAAATAGCAG CACTCTTTTG 
TGCCACTTCC GTTCCGTGGT CAGTTTGGAT TACATGGTCT TCTTCAGCTT CGTCACCTGG 
ATCCTCGTCC CCCTGGTTGT CATGTGTGTC ATCTACCTAG ACATCTTCTA CATCATCCGA 
AATAAGCTCA GTCAAAACCT GTCTGGCTTC AGAGAGACGC GTGCATTTTA TGGACGGGAG 
TTCAAGACAG CTAAGTCCCT GTTTCTGGTT CTCTTCTTGT TTGCGCTGTG CTGGCTGCCT 
TTGTCCATCA TCAATTTTGT TTCCTATTTT GATGTAAAGA TACCAGATGT CGCAATGTGC 
CTGGGGATCC TGTTGTCCCA CGCGAACTCC ATGATGAACC CTATTGTCTA CGCCTGCAAA 
ATAAAAAAGT TCAAAGAGAC CTACTTTCTG ATCCTCAGAG CTCTCAGGCT CTGTCAGACC 
TCAGATTCTT TGGACTCAAA CATGGAACAG ACTACTGAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001167640.1
CDS240..761
Translation

Target ORF information:

RefSeq Version NM_001174169.2
Organism Mus musculus(house mouse)
Definition Mus musculus transmembrane and immunoglobulin domain containing 3 (Tmigd3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001174169.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTTTGGCT GGAATAGAAA AGCAACCTTA GCGAGCTCTC AAAATAGCAG CACTCTTTTG 
TGCCACTTCC GTTCCGTGGT CAGTTTGGAT TACATGGTCT TCTTCAGCTT CGTCACCTGG 
ATCCTCGTCC CCCTGGTTGT CATGTGTGTC ATCTACCTAG ACATCTTCTA CATCATCCGA 
AATAAGCTCA GTCAAAACCT GTCTGGCTTC AGAGAGACGC GTGCATTTTA TGGACGGGAG 
TTCAAGACAG CTAAGTCCCT GTTTCTGGTT CTCTTCTTGT TTGCGCTGTG CTGGCTGCCT 
TTGTCCATCA TCAATTTTGT TTCCTATTTT GATGTAAAGA TACCAGATGT CGCAATGTGC 
CTGGGGATCC TGTTGTCCCA CGCGAACTCC ATGATGAACC CTATTGTCTA CGCCTGCAAA 
ATAAAAAAGT TCAAAGAGAC CTACTTTCTG ATCCTCAGAG CTCTCAGGCT CTGTCAGACC 
TCAGATTCTT TGGACTCAAA CATGGAACAG ACTACTGAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19830
Clone ID Related Accession (Same CDS sequence) NM_027025.4
Accession Version NM_027025.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-23
Organism Mus musculus(house mouse)
Product transmembrane domain-containing protein TMIGD3 isoform 2 precursor
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121847.2, AK005581.1 and BC038878.1. On Sep 24, 2013 this sequence version replaced NM_027025.3. Transcript Variant: This variant (2) encodes the longer isoform (2), which is a transmembrane and immunoglobulin domain-containing protein and it does not share any similarity with the adenosine A3 receptor. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGAGTTCC TCCTCCTTCT CTCCTTGGCT CTCTTTTCCG ATGCCATGGT CATGGATGAA 
AAGGTGAAAT CAGGTGTTGA GCTGGAGACA GCTTCTGCAG TCTGTGTCTA TGATGCCTAC 
TACAAGGACC ACACCAAGTA CTGGTGCCGT GGCTACTTCA GGGACTCGTG CAACATTATT 
GCCTTCACCC CTAATAGCAC CAACCGTGTG GCCCTGAAGG ATACAGGAAA CCAACTCATT 
ATCACTATAT CCTGCCTGGT CAAGGAGGAC ACAGGCTGGT ACTGGTGTGG TATCCAGCGG 
GACTTGGCCA GAGATGACAT GGATTTTACA CAGCTGATTG TGACTGACAA TAGAGAAGAC 
CGGGCCAATG GCTTTTCATC TGACCCTTCA GGCAATAGGA CCCGGAGCTG TAGGGCTTCC 
AAAGCTGTCC AAAAGGCAGA GGGCTCCCGA ATGTCCATTC TGATCATTTG TATACTGATT 
ACCAGTTTGG GGATCATCTT TATAATCAGT CACTTGTCCA GAGGAAGGCG AAGCCAAAGA 
AATAGAGAAG TTACTGGCAA AAGCATCAGT AGAAACCCAC AAGCAAGCCA AGGCCCGTCT 
ATGGTCTCCA TAACTTTGGC AAGGATTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_081301.2
CDS217..846
Translation

Target ORF information:

RefSeq Version NM_027025.4
Organism Mus musculus(house mouse)
Definition Mus musculus transmembrane and immunoglobulin domain containing 3 (Tmigd3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_027025.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGAGTTCC TCCTCCTTCT CTCCTTGGCT CTCTTTTCCG ATGCCATGGT CATGGATGAA 
AAGGTGAAAT CAGGTGTTGA GCTGGAGACA GCTTCTGCAG TCTGTGTCTA TGATGCCTAC 
TACAAGGACC ACACCAAGTA CTGGTGCCGT GGCTACTTCA GGGACTCGTG CAACATTATT 
GCCTTCACCC CTAATAGCAC CAACCGTGTG GCCCTGAAGG ATACAGGAAA CCAACTCATT 
ATCACTATAT CCTGCCTGGT CAAGGAGGAC ACAGGCTGGT ACTGGTGTGG TATCCAGCGG 
GACTTGGCCA GAGATGACAT GGATTTTACA CAGCTGATTG TGACTGACAA TAGAGAAGAC 
CGGGCCAATG GCTTTTCATC TGACCCTTCA GGCAATAGGA CCCGGAGCTG TAGGGCTTCC 
AAAGCTGTCC AAAAGGCAGA GGGCTCCCGA ATGTCCATTC TGATCATTTG TATACTGATT 
ACCAGTTTGG GGATCATCTT TATAATCAGT CACTTGTCCA GAGGAAGGCG AAGCCAAAGA 
AATAGAGAAG TTACTGGCAA AAGCATCAGT AGAAACCCAC AAGCAAGCCA AGGCCCGTCT 
ATGGTCTCCA TAACTTTGGC AAGGATTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Calreticulin mutant mice develop essential thrombocythemia that is ameliorated by the JAK inhibitor ruxolitinib.
Leukemia31(5)1136-1144(2017 May)
Shide K,Kameda T,Yamaji T,Sekine M,Inada N,Kamiunten A,Akizuki K,Nakamura K,Hidaka T,Kubuki Y,Shimoda H,Kitanaka A,Honda A,Sawaguchi A,Abe H,Miike T,Iwakiri H,Tahara Y,Sueta M,Hasuike S,Yamamoto S,Nagata K,Shimoda K