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Prpf4 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Prpf4
Entrez Gene ID 70052
Full Name pre-mRNA processing factor 4
Synonyms 1600015H11Rik,AI874830,AW047464,bN189G18.1
General protein information
Preferred Names

pre-mRNA processing factor 4

Names

U4/U6 small nuclear ribonucleoprotein Prp4
U4/U6 snRNP 60 kDa protein
WD splicing factor Prp4
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

4

4|4 B3

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_030253770.1 XP_030109630.1 U4/U6 small nuclear ribonucleoprotein Prp4 isoform X1
XM_006538249.3 XP_006538312.1 U4/U6 small nuclear ribonucleoprotein Prp4 isoform X1
NM_027297.3 NP_081573.1 U4/U6 small nuclear ribonucleoprotein Prp4
XM_006538249.2 XP_006538312.1 U4/U6 small nuclear ribonucleoprotein Prp4 isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) PRPF4 NP_001231855.1
Danio rerio (zebrafish) prpf4 NP_956049.1
Danio rerio (zebrafish) LOC100334455 XP_002662201.1
Caenorhabditis elegans (roundworm) prp-4 NP_492363.1
Kluyveromyces lactis KLLA0E04819g XP_454163.1
Neurospora crassa NCU11251 XP_001728376.1
Canis lupus familiaris (dog) PRPF4 XP_532038.2
Drosophila melanogaster (fruit fly) U4-U6-60K NP_648990.1
Eremothecium gossypii AGOS_AEL269C NP_984591.1
Schizosaccharomyces pombe (fission yeast) SPAC227.12 NP_592966.1
Arabidopsis thaliana (thale cress) AT2G05720 NP_178635.1
Rattus norvegicus (Norway rat) Prpf4 NP_001100129.1
Arabidopsis thaliana (thale cress) LIS NP_181681.1
PRPF4 XP_001102859.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP002144 XP_308051.4
Magnaporthe oryzae (rice blast fungus) MGG_01259 XP_003714171.1
Xenopus tropicalis (tropical clawed frog) prpf4 NP_989058.1
Pan troglodytes (chimpanzee) PRPF4 XP_520198.3
Bos taurus (cattle) PRPF4 NP_001029502.1
Mus musculus (house mouse) Prpf4 NP_081573.1
Gallus gallus (chicken) PRPF4 XP_415544.3
Saccharomyces cerevisiae (baker's yeast) PRP4 NP_015504.1

Gene Ontology


Bibliography

Related articles in PubMed

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prpf4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prpf4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu33134 XM_030253770.1
Latest version!		 Mus musculus pre-mRNA processing factor 4 (Prpf4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OMu33134 XM_006538249.3
Latest version!		 Mus musculus pre-mRNA processing factor 4 (Prpf4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OMu14500 NM_027297.3
Latest version!		 Mus musculus pre-mRNA processing factor 4 (Prpf4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $300.30
$429.00
View Old Accession Versions >>
OMu33134 XM_006538249.2 Mus musculus pre-mRNA processing factor 4 (Prpf4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu33134
Clone ID Related Accession (Same CDS sequence) XM_030253770.1 , XM_006538249.3 , XM_006538249.2
Accession Version XM_006538249.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 840bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product U4/U6 small nuclear ribonucleoprotein Prp4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCTTTCCT TTGTGTTTGA TAGGAGTGGG CTTTGCAAGC TCTGGTCTGT CCCTGACTGC 
AGCCTCCTTC ACACTCTTCG AGGCCATAAC ACAAATGTAG GAGCAATTGT ATTCCATCCC 
AAATCCACTG TCTCGTTGGA CCAGAAGGAT GTCAACCTGG CCTCTTGTGC TGCTGATGGT 
TCTGTGAAGC TTTGGAGCCT TGACAGCGAT GAACCAGTGG CAGATATTGA AGGCCATACA 
GTGCGTGTAG CTCGGGTAAT GTGGCATCCA TCAGGGCGTT TCCTGGGCAC AACCTGTTAT 
GACCGTTCAT GGCGCTTATG GGATTTAGAG GCTCAAGAGG AGATCCTGCA CCAGGAAGGC 
CACAGCATGG GTGTGTATGA CATTGCCTTC CATCAAGATG GCTCTTTGGC TGGCACTGGG 
GGACTGGATG CATTTGGCCG GGTTTGGGAC CTACGCACAG GACGCTGCAT TATGTTCCTG 
GAAGGCCACC TGAAGGAAAT CTATGGGATA AATTTCAGCC CCAATGGCTA CCACATTGCC 
ACTGGCAGTG GTGACAACAC CTGCAAAGTG TGGGACCTTC GGCAGCGACG CTGTGTCTAC 
ACCATCCCCG CCCATCAGAA CTTAGTGACC GGTGTCAAAT TTGAGCCTAT CCATGGGGAC 
TTCTTGCTCA CAGGTGCCTA TGACAATACA GCCAAGATCT GGACCCACCC AGGCTGGTCC 
CCACTGAAGA CCCTGGCTGG TCATGAGGGC AAAGTGATGG GCCTGGACAT TTCTTCTGAT 
GGGCAGCTCA TAGCCACGTG CTCCTATGAC AGAACCTTCA AGCTCTGGAT GGCAGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006538312.1
CDS107..946
Translation

Target ORF information:

RefSeq Version XM_006538249.2
Organism Mus musculus(house mouse)
Definition Mus musculus pre-mRNA processing factor 4 (Prpf4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006538249.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCTTTCCT TTGTGTTTGA TAGGAGTGGG CTTTGCAAGC TCTGGTCTGT CCCTGACTGC 
AGCCTCCTTC ACACTCTTCG AGGCCATAAC ACAAATGTAG GAGCAATTGT ATTCCATCCC 
AAATCCACTG TCTCGTTGGA CCAGAAGGAT GTCAACCTGG CCTCTTGTGC TGCTGATGGT 
TCTGTGAAGC TTTGGAGCCT TGACAGCGAT GAACCAGTGG CAGATATTGA AGGCCATACA 
GTGCGTGTAG CTCGGGTAAT GTGGCATCCA TCAGGGCGTT TCCTGGGCAC AACCTGTTAT 
GACCGTTCAT GGCGCTTATG GGATTTAGAG GCTCAAGAGG AGATCCTGCA CCAGGAAGGC 
CACAGCATGG GTGTGTATGA CATTGCCTTC CATCAAGATG GCTCTTTGGC TGGCACTGGG 
GGACTGGATG CATTTGGCCG GGTTTGGGAC CTACGCACAG GACGCTGCAT TATGTTCCTG 
GAAGGCCACC TGAAGGAAAT CTATGGGATA AATTTCAGCC CCAATGGCTA CCACATTGCC 
ACTGGCAGTG GTGACAACAC CTGCAAAGTG TGGGACCTTC GGCAGCGACG CTGTGTCTAC 
ACCATCCCCG CCCATCAGAA CTTAGTGACC GGTGTCAAAT TTGAGCCTAT CCATGGGGAC 
TTCTTGCTCA CAGGTGCCTA TGACAATACA GCCAAGATCT GGACCCACCC AGGCTGGTCC 
CCACTGAAGA CCCTGGCTGG TCATGAGGGC AAAGTGATGG GCCTGGACAT TTCTTCTGAT 
GGGCAGCTCA TAGCCACGTG CTCCTATGAC AGAACCTTCA AGCTCTGGAT GGCAGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu33134
Clone ID Related Accession (Same CDS sequence) XM_030253770.1 , XM_006538249.3 , XM_006538249.2
Accession Version XM_030253770.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 840bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product U4/U6 small nuclear ribonucleoprotein Prp4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCTTTCCT TTGTGTTTGA TAGGAGTGGG CTTTGCAAGC TCTGGTCTGT CCCTGACTGC 
AGCCTCCTTC ACACTCTTCG AGGCCATAAC ACAAATGTAG GAGCAATTGT ATTCCATCCC 
AAATCCACTG TCTCGTTGGA CCAGAAGGAT GTCAACCTGG CCTCTTGTGC TGCTGATGGT 
TCTGTGAAGC TTTGGAGCCT TGACAGCGAT GAACCAGTGG CAGATATTGA AGGCCATACA 
GTGCGTGTAG CTCGGGTAAT GTGGCATCCA TCAGGGCGTT TCCTGGGCAC AACCTGTTAT 
GACCGTTCAT GGCGCTTATG GGATTTAGAG GCTCAAGAGG AGATCCTGCA CCAGGAAGGC 
CACAGCATGG GTGTGTATGA CATTGCCTTC CATCAAGATG GCTCTTTGGC TGGCACTGGG 
GGACTGGATG CATTTGGCCG GGTTTGGGAC CTACGCACAG GACGCTGCAT TATGTTCCTG 
GAAGGCCACC TGAAGGAAAT CTATGGGATA AATTTCAGCC CCAATGGCTA CCACATTGCC 
ACTGGCAGTG GTGACAACAC CTGCAAAGTG TGGGACCTTC GGCAGCGACG CTGTGTCTAC 
ACCATCCCCG CCCATCAGAA CTTAGTGACC GGTGTCAAAT TTGAGCCTAT CCATGGGGAC 
TTCTTGCTCA CAGGTGCCTA TGACAATACA GCCAAGATCT GGACCCACCC AGGCTGGTCC 
CCACTGAAGA CCCTGGCTGG TCATGAGGGC AAAGTGATGG GCCTGGACAT TTCTTCTGAT 
GGGCAGCTCA TAGCCACGTG CTCCTATGAC AGAACCTTCA AGCTCTGGAT GGCAGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_030109630.1
CDS850..1689
Translation

Target ORF information:

RefSeq Version XM_030253770.1
Organism Mus musculus(house mouse)
Definition Mus musculus pre-mRNA processing factor 4 (Prpf4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_030253770.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCTTTCCT TTGTGTTTGA TAGGAGTGGG CTTTGCAAGC TCTGGTCTGT CCCTGACTGC 
AGCCTCCTTC ACACTCTTCG AGGCCATAAC ACAAATGTAG GAGCAATTGT ATTCCATCCC 
AAATCCACTG TCTCGTTGGA CCAGAAGGAT GTCAACCTGG CCTCTTGTGC TGCTGATGGT 
TCTGTGAAGC TTTGGAGCCT TGACAGCGAT GAACCAGTGG CAGATATTGA AGGCCATACA 
GTGCGTGTAG CTCGGGTAAT GTGGCATCCA TCAGGGCGTT TCCTGGGCAC AACCTGTTAT 
GACCGTTCAT GGCGCTTATG GGATTTAGAG GCTCAAGAGG AGATCCTGCA CCAGGAAGGC 
CACAGCATGG GTGTGTATGA CATTGCCTTC CATCAAGATG GCTCTTTGGC TGGCACTGGG 
GGACTGGATG CATTTGGCCG GGTTTGGGAC CTACGCACAG GACGCTGCAT TATGTTCCTG 
GAAGGCCACC TGAAGGAAAT CTATGGGATA AATTTCAGCC CCAATGGCTA CCACATTGCC 
ACTGGCAGTG GTGACAACAC CTGCAAAGTG TGGGACCTTC GGCAGCGACG CTGTGTCTAC 
ACCATCCCCG CCCATCAGAA CTTAGTGACC GGTGTCAAAT TTGAGCCTAT CCATGGGGAC 
TTCTTGCTCA CAGGTGCCTA TGACAATACA GCCAAGATCT GGACCCACCC AGGCTGGTCC 
CCACTGAAGA CCCTGGCTGG TCATGAGGGC AAAGTGATGG GCCTGGACAT TTCTTCTGAT 
GGGCAGCTCA TAGCCACGTG CTCCTATGAC AGAACCTTCA AGCTCTGGAT GGCAGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu33134
Clone ID Related Accession (Same CDS sequence) XM_030253770.1 , XM_006538249.3 , XM_006538249.2
Accession Version XM_006538249.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 840bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product U4/U6 small nuclear ribonucleoprotein Prp4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538249.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCTTTCCT TTGTGTTTGA TAGGAGTGGG CTTTGCAAGC TCTGGTCTGT CCCTGACTGC 
AGCCTCCTTC ACACTCTTCG AGGCCATAAC ACAAATGTAG GAGCAATTGT ATTCCATCCC 
AAATCCACTG TCTCGTTGGA CCAGAAGGAT GTCAACCTGG CCTCTTGTGC TGCTGATGGT 
TCTGTGAAGC TTTGGAGCCT TGACAGCGAT GAACCAGTGG CAGATATTGA AGGCCATACA 
GTGCGTGTAG CTCGGGTAAT GTGGCATCCA TCAGGGCGTT TCCTGGGCAC AACCTGTTAT 
GACCGTTCAT GGCGCTTATG GGATTTAGAG GCTCAAGAGG AGATCCTGCA CCAGGAAGGC 
CACAGCATGG GTGTGTATGA CATTGCCTTC CATCAAGATG GCTCTTTGGC TGGCACTGGG 
GGACTGGATG CATTTGGCCG GGTTTGGGAC CTACGCACAG GACGCTGCAT TATGTTCCTG 
GAAGGCCACC TGAAGGAAAT CTATGGGATA AATTTCAGCC CCAATGGCTA CCACATTGCC 
ACTGGCAGTG GTGACAACAC CTGCAAAGTG TGGGACCTTC GGCAGCGACG CTGTGTCTAC 
ACCATCCCCG CCCATCAGAA CTTAGTGACC GGTGTCAAAT TTGAGCCTAT CCATGGGGAC 
TTCTTGCTCA CAGGTGCCTA TGACAATACA GCCAAGATCT GGACCCACCC AGGCTGGTCC 
CCACTGAAGA CCCTGGCTGG TCATGAGGGC AAAGTGATGG GCCTGGACAT TTCTTCTGAT 
GGGCAGCTCA TAGCCACGTG CTCCTATGAC AGAACCTTCA AGCTCTGGAT GGCAGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006538312.1
CDS869..1708
Translation

Target ORF information:

RefSeq Version XM_006538249.3
Organism Mus musculus(house mouse)
Definition Mus musculus pre-mRNA processing factor 4 (Prpf4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006538249.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCTTTCCT TTGTGTTTGA TAGGAGTGGG CTTTGCAAGC TCTGGTCTGT CCCTGACTGC 
AGCCTCCTTC ACACTCTTCG AGGCCATAAC ACAAATGTAG GAGCAATTGT ATTCCATCCC 
AAATCCACTG TCTCGTTGGA CCAGAAGGAT GTCAACCTGG CCTCTTGTGC TGCTGATGGT 
TCTGTGAAGC TTTGGAGCCT TGACAGCGAT GAACCAGTGG CAGATATTGA AGGCCATACA 
GTGCGTGTAG CTCGGGTAAT GTGGCATCCA TCAGGGCGTT TCCTGGGCAC AACCTGTTAT 
GACCGTTCAT GGCGCTTATG GGATTTAGAG GCTCAAGAGG AGATCCTGCA CCAGGAAGGC 
CACAGCATGG GTGTGTATGA CATTGCCTTC CATCAAGATG GCTCTTTGGC TGGCACTGGG 
GGACTGGATG CATTTGGCCG GGTTTGGGAC CTACGCACAG GACGCTGCAT TATGTTCCTG 
GAAGGCCACC TGAAGGAAAT CTATGGGATA AATTTCAGCC CCAATGGCTA CCACATTGCC 
ACTGGCAGTG GTGACAACAC CTGCAAAGTG TGGGACCTTC GGCAGCGACG CTGTGTCTAC 
ACCATCCCCG CCCATCAGAA CTTAGTGACC GGTGTCAAAT TTGAGCCTAT CCATGGGGAC 
TTCTTGCTCA CAGGTGCCTA TGACAATACA GCCAAGATCT GGACCCACCC AGGCTGGTCC 
CCACTGAAGA CCCTGGCTGG TCATGAGGGC AAAGTGATGG GCCTGGACAT TTCTTCTGAT 
GGGCAGCTCA TAGCCACGTG CTCCTATGAC AGAACCTTCA AGCTCTGGAT GGCAGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu14500
Clone ID Related Accession (Same CDS sequence) NM_027297.3
Accession Version NM_027297.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1566bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-24
Organism Mus musculus(house mouse)
Product U4/U6 small nuclear ribonucleoprotein Prp4
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732548.9, AK167216.1 and AL732594.5. On Jan 13, 2006 this sequence version replaced NM_027297.2. ##Evidence-Data-START## Transcript exon combination :: AK085174.1, AK169532.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCCTCCT CACGAGCCTC CTCCACGACA ACCAAAACCA AAGCGCCTGA CGATTTAGTT 
GCTCCCGTTG TGAAGAAACC ACACATCTAC TATGGAAGCT TGGAAGAGAA AGAGCGGGAA 
CGCCTGGCCA AAGGAGAGTC TGGGATTTTG GGAAAAGAAG GACTCAAAGC CGGCATTGAA 
GCAGGAAATA TTAATATAAC ATCCGGAGAA GTGTTTGAAA TTGAGGAGCA CATCAGTGAG 
CGGCAGGCAG AAGTCTTGGC AGAGTTTGAA AGAAGAAAGC GAGCCCGGCA GATCAATGTT 
TCCACAGATG ACTCAGAGGT CAAGGCTTGC CTTCGAGCCT TGGGAGAGCC TATCACGCTT 
TTTGGAGAGG GCCCTGCTGA ACGAAGAGAA AGGTTAAGAA ATATTCTCTC AGTGGTCGGG 
ACTGATGCCT TAAAAAAGAC GAAAAAAGAT GATGAAAAGT CTAAGAAGTC AAAGGAGGAG 
TATCAGCAAA CCTGGTACCA TGAAGGGCCA AATAGCCTGA AGGTGGCCAG ATTGTGGATT 
GCTAATTATT CACTCCCCAG GGCAATGAAA CGCTTAGAGG AGGCCCGTCT CCATAAGGAG 
ATTCCTGAGA CAACCAGAAC CTCCCAGATG CAGGAGCTGC ACAAGTCTCT CCGGTCTTTG 
AATAATTTCT GCAGTCAGAT TGGAGATGAC CGGCCGATCT CCTACTGCCA CTTCAGCCCC 
AATTCTAAAA TGCTAGCCAC AGCTTGTTGG AGTGGGCTTT GCAAGCTCTG GTCTGTCCCT 
GACTGCAGCC TCCTTCACAC TCTTCGAGGC CATAACACAA ATGTAGGAGC AATTGTATTC 
CATCCCAAAT CCACTGTCTC GTTGGACCAG AAGGATGTCA ACCTGGCCTC TTGTGCTGCT 
GATGGTTCTG TGAAGCTTTG GAGCCTTGAC AGCGATGAAC CAGTGGCAGA TATTGAAGGC 
CATACAGTGC GTGTAGCTCG GGTAATGTGG CATCCATCAG GGCGTTTCCT GGGCACAACC 
TGTTATGACC GTTCATGGCG CTTATGGGAT TTAGAGGCTC AAGAGGAGAT CCTGCACCAG 
GAAGGCCACA GCATGGGTGT GTATGACATT GCCTTCCATC AAGATGGCTC TTTGGCTGGC 
ACTGGGGGAC TGGATGCATT TGGCCGGGTT TGGGACCTAC GCACAGGACG CTGCATTATG 
TTCCTGGAAG GCCACCTGAA GGAAATCTAT GGGATAAATT TCAGCCCCAA TGGCTACCAC 
ATTGCCACTG GCAGTGGTGA CAACACCTGC AAAGTGTGGG ACCTTCGGCA GCGACGCTGT 
GTCTACACCA TCCCCGCCCA TCAGAACTTA GTGACCGGTG TCAAATTTGA GCCTATCCAT 
GGGGACTTCT TGCTCACAGG TGCCTATGAC AATACAGCCA AGATCTGGAC CCACCCAGGC 
TGGTCCCCAC TGAAGACCCT GGCTGGTCAT GAGGGCAAAG TGATGGGCCT GGACATTTCT 
TCTGATGGGC AGCTCATAGC CACGTGCTCC TATGACAGAA CCTTCAAGCT CTGGATGGCA 
GAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_081573.1
CDS93..1658
Translation

Target ORF information:

RefSeq Version NM_027297.3
Organism Mus musculus(house mouse)
Definition Mus musculus pre-mRNA processing factor 4 (Prpf4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_027297.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCCTCCT CACGAGCCTC CTCCACGACA ACCAAAACCA AAGCGCCTGA CGATTTAGTT 
GCTCCCGTTG TGAAGAAACC ACACATCTAC TATGGAAGCT TGGAAGAGAA AGAGCGGGAA 
CGCCTGGCCA AAGGAGAGTC TGGGATTTTG GGAAAAGAAG GACTCAAAGC CGGCATTGAA 
GCAGGAAATA TTAATATAAC ATCCGGAGAA GTGTTTGAAA TTGAGGAGCA CATCAGTGAG 
CGGCAGGCAG AAGTCTTGGC AGAGTTTGAA AGAAGAAAGC GAGCCCGGCA GATCAATGTT 
TCCACAGATG ACTCAGAGGT CAAGGCTTGC CTTCGAGCCT TGGGAGAGCC TATCACGCTT 
TTTGGAGAGG GCCCTGCTGA ACGAAGAGAA AGGTTAAGAA ATATTCTCTC AGTGGTCGGG 
ACTGATGCCT TAAAAAAGAC GAAAAAAGAT GATGAAAAGT CTAAGAAGTC AAAGGAGGAG 
TATCAGCAAA CCTGGTACCA TGAAGGGCCA AATAGCCTGA AGGTGGCCAG ATTGTGGATT 
GCTAATTATT CACTCCCCAG GGCAATGAAA CGCTTAGAGG AGGCCCGTCT CCATAAGGAG 
ATTCCTGAGA CAACCAGAAC CTCCCAGATG CAGGAGCTGC ACAAGTCTCT CCGGTCTTTG 
AATAATTTCT GCAGTCAGAT TGGAGATGAC CGGCCGATCT CCTACTGCCA CTTCAGCCCC 
AATTCTAAAA TGCTAGCCAC AGCTTGTTGG AGTGGGCTTT GCAAGCTCTG GTCTGTCCCT 
GACTGCAGCC TCCTTCACAC TCTTCGAGGC CATAACACAA ATGTAGGAGC AATTGTATTC 
CATCCCAAAT CCACTGTCTC GTTGGACCAG AAGGATGTCA ACCTGGCCTC TTGTGCTGCT 
GATGGTTCTG TGAAGCTTTG GAGCCTTGAC AGCGATGAAC CAGTGGCAGA TATTGAAGGC 
CATACAGTGC GTGTAGCTCG GGTAATGTGG CATCCATCAG GGCGTTTCCT GGGCACAACC 
TGTTATGACC GTTCATGGCG CTTATGGGAT TTAGAGGCTC AAGAGGAGAT CCTGCACCAG 
GAAGGCCACA GCATGGGTGT GTATGACATT GCCTTCCATC AAGATGGCTC TTTGGCTGGC 
ACTGGGGGAC TGGATGCATT TGGCCGGGTT TGGGACCTAC GCACAGGACG CTGCATTATG 
TTCCTGGAAG GCCACCTGAA GGAAATCTAT GGGATAAATT TCAGCCCCAA TGGCTACCAC 
ATTGCCACTG GCAGTGGTGA CAACACCTGC AAAGTGTGGG ACCTTCGGCA GCGACGCTGT 
GTCTACACCA TCCCCGCCCA TCAGAACTTA GTGACCGGTG TCAAATTTGA GCCTATCCAT 
GGGGACTTCT TGCTCACAGG TGCCTATGAC AATACAGCCA AGATCTGGAC CCACCCAGGC 
TGGTCCCCAC TGAAGACCCT GGCTGGTCAT GAGGGCAAAG TGATGGGCCT GGACATTTCT 
TCTGATGGGC AGCTCATAGC CACGTGCTCC TATGACAGAA CCTTCAAGCT CTGGATGGCA 
GAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE