Afap1 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Afap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Afap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu04097 NM_027373.2
Latest version!
Mus musculus actin filament associated protein 1 (Afap1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OMu35188 XM_006504100.3
Latest version!
Mus musculus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu35186 XM_006504098.3
Latest version!
Mus musculus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu35188 XM_030254799.1
Latest version!
Mus musculus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu89013 XM_030254800.1
Latest version!
Mus musculus actin filament associated protein 1 (Afap1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OMu35186 XM_006504098.2 Mus musculus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu04097
    Clone ID Related Accession (Same CDS sequence) NM_027373.2
    Accession Version NM_027373.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Mus musculus(house mouse)
    Product actin filament-associated protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC050814.1 and AC127275.4. On Nov 1, 2005 this sequence version replaced NM_027373.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147706.1, AK129483.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGAGT TAATAGTTGA ACTCCGCCTC TTTCTGGAGC TCCTGGACCA CGAGTACCTC 
    ACATCAACTG TCAGAGAGAA AAAGGCTGTG CTCACCAACA TCCTGCTGCG CCTGCAGTCA
    TCCAAGGGCT TTGAAGTGAA GGACCATGCT CAGAAGGCAG AGGCCAACAA CCTGCCGGCA
    CCACCCCAGA TGCCTCTGCC GGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGCGGGCCT
    CCGCCTTTAC CAACGTCCTC TCTCCCAGAG GGTTACTATG AGGAAGCCGT GCCGCTGAGC
    CCTGGGAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGATGCCAT GAGCAGTTCC
    TATGAGTCAT ATGATGAAGA GGAAGAGGAC GGGAAGGGGA AGAAAACTCG GCATCAGTGG
    CCGTCAGAGG AGGCCTCTAT GGACCTGGTG AAAGACGCCA AGATCTGTGC CTTCCTGCTG
    CGCAAGAAAC GCTTTGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG
    CTGTGCTATA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCGCT GCAGGGCTGC
    AGTATCACAT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG
    CAAGGCACAG ACCCTCTGGT TCTAGCTGTT CAGAGCAAGG AACAGGCTGA GCAGTGGTTG
    AAGGTAATCA AAGAAGCCTA CAGTGGCTGT AGCGGGCCCG TGGATCCGGA ATGTTCTCCA
    CCACCCAGCA CCAGCGCCCC CGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG
    AGGCCCAGCT CTGATGGAGA AGGTGGTGTG GAAAACGGAG TCACCACGTG TAATGGAAAA
    GAACAAGCAA AGAGGAAGAA ACCTTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC
    ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG
    CAGACATCAT CAGCAGAGGA GGATGTGCCC ACTTGTGGCT ACCTGAATGT GCTCTCTAAC
    AGCCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AACTCATTCT CCACAAGGAC
    AGGGCTGACC TGAAGACCCA CCTCGTCTCC ATCCCCCTCC GTGGCTGCGA GGTGATCCCG
    GGCCTGGATT CTAAGCACCC GTTGACCTTC CGGCTGCTTC GAAACGGACA GGAGGTGGCT
    GTTCTAGAGG CATCATCGTC TGAAGACATG GGCAGGTGGA TTGGCATCTT GCTGGCTGAG
    ACGGGGTCCT CCACGGACCC AGGAGCGCTG CACTACGACT ACATCGATGT GGAGATGTCA
    GCAAATGTCA TCCAGACGGC GAAACAGACC TTCTGCTTTA TGAACAGGCG GGCAGTGTCC
    ACTAGCCCAT ACCTGGGGAG CCTCTCCAAC GGCTACGCCC ATCCCAGTGG GACAGCACTA
    CACTATGATG ACGTCCCCTG TGTCAACGGC TCGTTGAAGA ACAAAAAACC GCCAGCGTCA
    TCTAATGGGG TTCCTGTAAA GGGGAAGGCT CCCAGCAGTC AGCAAAAAAA GGTGGAAACT
    GCAGGTGGTG TGAAACGGAC AGCATCAAAT GCTGAGCAGT ACAAATATGG GAAGAACCGG
    GTAGAAGCAG ATGCCAAGCG GTTACAGTCC AAAGAGGAAG AGCTGCTGAA GAGGAAAGAG
    GCCCTACGGA ACAGGCTGGC ACAGCTGCGG AAAGAGAGGA AGGACCTGAG GGCCGCCATC
    GAAGTGAATG CAGGCAGGAA GACCCAGGCA GCTCTGGAGG ACAAGTTGAA GAGGCTGGAG
    GAAGAGTGCA AGCAGAGGGA GGCGGAGCGT GTCAGCCTGG AGCTGGAGCT GACCGAGGTC
    AAGGAGAGCC TGAAGAAGGC CTTGGCGGGT GGCGTCACCC TAGGACTGGC CATCGAGCCC
    AGGTCAGGGA CATCCAGTCC ACAGTCTCCT GTGTTTCGGC ATCGGACTCT GGAAAATTCT
    CCCATCTCCA GCTGTGATAC AAGCGATGCT GAGGGGCCTT TGCCTGTGAA CAGTGCAGCC
    GTCCTGAAGA AGAGCCAGCC GTCCTCAGGC AGCTCCCCTT GCCGTGGCCA CGTGCTGCAG
    AAGGCCAAGG AGTGGGAGCT GAAGAATGGG ACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_081649.1
    CDS225..2420
    Translation

    Target ORF information:

    RefSeq Version NM_027373.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus actin filament associated protein 1 (Afap1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_027373.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGAGT TAATAGTTGA ACTCCGCCTC TTTCTGGAGC TCCTGGACCA CGAGTACCTC 
    ACATCAACTG TCAGAGAGAA AAAGGCTGTG CTCACCAACA TCCTGCTGCG CCTGCAGTCA
    TCCAAGGGCT TTGAAGTGAA GGACCATGCT CAGAAGGCAG AGGCCAACAA CCTGCCGGCA
    CCACCCCAGA TGCCTCTGCC GGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGCGGGCCT
    CCGCCTTTAC CAACGTCCTC TCTCCCAGAG GGTTACTATG AGGAAGCCGT GCCGCTGAGC
    CCTGGGAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGATGCCAT GAGCAGTTCC
    TATGAGTCAT ATGATGAAGA GGAAGAGGAC GGGAAGGGGA AGAAAACTCG GCATCAGTGG
    CCGTCAGAGG AGGCCTCTAT GGACCTGGTG AAAGACGCCA AGATCTGTGC CTTCCTGCTG
    CGCAAGAAAC GCTTTGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG
    CTGTGCTATA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCGCT GCAGGGCTGC
    AGTATCACAT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG
    CAAGGCACAG ACCCTCTGGT TCTAGCTGTT CAGAGCAAGG AACAGGCTGA GCAGTGGTTG
    AAGGTAATCA AAGAAGCCTA CAGTGGCTGT AGCGGGCCCG TGGATCCGGA ATGTTCTCCA
    CCACCCAGCA CCAGCGCCCC CGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG
    AGGCCCAGCT CTGATGGAGA AGGTGGTGTG GAAAACGGAG TCACCACGTG TAATGGAAAA
    GAACAAGCAA AGAGGAAGAA ACCTTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC
    ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG
    CAGACATCAT CAGCAGAGGA GGATGTGCCC ACTTGTGGCT ACCTGAATGT GCTCTCTAAC
    AGCCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AACTCATTCT CCACAAGGAC
    AGGGCTGACC TGAAGACCCA CCTCGTCTCC ATCCCCCTCC GTGGCTGCGA GGTGATCCCG
    GGCCTGGATT CTAAGCACCC GTTGACCTTC CGGCTGCTTC GAAACGGACA GGAGGTGGCT
    GTTCTAGAGG CATCATCGTC TGAAGACATG GGCAGGTGGA TTGGCATCTT GCTGGCTGAG
    ACGGGGTCCT CCACGGACCC AGGAGCGCTG CACTACGACT ACATCGATGT GGAGATGTCA
    GCAAATGTCA TCCAGACGGC GAAACAGACC TTCTGCTTTA TGAACAGGCG GGCAGTGTCC
    ACTAGCCCAT ACCTGGGGAG CCTCTCCAAC GGCTACGCCC ATCCCAGTGG GACAGCACTA
    CACTATGATG ACGTCCCCTG TGTCAACGGC TCGTTGAAGA ACAAAAAACC GCCAGCGTCA
    TCTAATGGGG TTCCTGTAAA GGGGAAGGCT CCCAGCAGTC AGCAAAAAAA GGTGGAAACT
    GCAGGTGGTG TGAAACGGAC AGCATCAAAT GCTGAGCAGT ACAAATATGG GAAGAACCGG
    GTAGAAGCAG ATGCCAAGCG GTTACAGTCC AAAGAGGAAG AGCTGCTGAA GAGGAAAGAG
    GCCCTACGGA ACAGGCTGGC ACAGCTGCGG AAAGAGAGGA AGGACCTGAG GGCCGCCATC
    GAAGTGAATG CAGGCAGGAA GACCCAGGCA GCTCTGGAGG ACAAGTTGAA GAGGCTGGAG
    GAAGAGTGCA AGCAGAGGGA GGCGGAGCGT GTCAGCCTGG AGCTGGAGCT GACCGAGGTC
    AAGGAGAGCC TGAAGAAGGC CTTGGCGGGT GGCGTCACCC TAGGACTGGC CATCGAGCCC
    AGGTCAGGGA CATCCAGTCC ACAGTCTCCT GTGTTTCGGC ATCGGACTCT GGAAAATTCT
    CCCATCTCCA GCTGTGATAC AAGCGATGCT GAGGGGCCTT TGCCTGTGAA CAGTGCAGCC
    GTCCTGAAGA AGAGCCAGCC GTCCTCAGGC AGCTCCCCTT GCCGTGGCCA CGTGCTGCAG
    AAGGCCAAGG AGTGGGAGCT GAAGAATGGG ACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35186
    Clone ID Related Accession (Same CDS sequence) XM_006504098.2 , XM_006504098.3
    Accession Version XM_006504098.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product actin filament-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504098.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAGAGT TAATAGTTGA ACTCCGCCTC TTTCTGGAGC TCCTGGACCA CGAGTACCTC 
    ACATCAACTG TCAGAGAGAA AAAGGCTGTG CTCACCAACA TCCTGCTGCG CCTGCAGTCA
    TCCAAGGGCT TTGAAGTGAA GGACCATGCT CAGAAGGCAG AGGCCAACAA CCTGCCGGCA
    CCACCCCAGA TGCCTCTGCC GGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGCGGGCCT
    CCGCCTTTAC CAACGTCCTC TCTCCCAGAG GGTTACTATG AGGAAGCCGT GCCGCTGAGC
    CCTGGGAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGATGCCAT GAGCAGTTCC
    TATGAGTCAT ATGATGAAGA GGAAGAGGAC GGGAAGGGGA AGAAAACTCG GCATCAGTGG
    CCGTCAGAGG AGGCCTCTAT GGACCTGGTG AAAGACGCCA AGATCTGTGC CTTCCTGCTG
    CGCAAGAAAC GCTTTGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG
    CTGTGCTATA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCGCT GCAGGGCTGC
    AGTATCACAT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG
    CAAGGCACAG ACCCTCTGGT TCTAGCTGTT CAGAGCAAGG AACAGGCTGA GCAGTGGTTG
    AAGGTAATCA AAGAAGCCTA CAGTGGCTGT AGCGGGCCCG TGGATCCGGA ATGTTCTCCA
    CCACCCAGCA CCAGCGCCCC CGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG
    AGGCCCAGCT CTGATGGAGA AGGTGGTGTG GAAAACGGAG TCACCACGTG TAATGGAAAA
    GAACAAGCAA AGAGGAAGAA ACCTTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC
    ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG
    CAGACATCAT CAGCAGAGGA GGATGTGCCC ACTTGTGGCT ACCTGAATGT GCTCTCTAAC
    AGCCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AACTCATTCT CCACAAGGAC
    AGGGCTGACC TGAAGACCCA CCTCGTCTCC ATCCCCCTCC GTGGCTGCGA GGTGATCCCG
    GGCCTGGATT CTAAGCACCC GTTGACCTTC CGGCTGCTTC GAAACGGACA GGAGGTGGCT
    GTTCTAGAGG CATCATCGTC TGAAGACATG GGCAGGTGGA TTGGCATCTT GCTGGCTGAG
    ACGGGGTCCT CCACGGACCC AGGAGCGCTG CACTACGACT ACATCGATGT GGAGATGTCA
    GCAAATGTCA TCCAGACGGC GAAACAGACC TTCTGCTTTA TGAACAGGCG GGCAGTGTCC
    ACTAGCCCAT ACCTGGGGAG CCTCTCCAAC GGCTACGCCC ATCCCAGTGG GACAGCACTA
    CACTATGATG ACGTCCCCTG TGTCAACGGC TCGTGGGACA TGGATGGCGG CTTTCCTGGC
    TGTGTGAGCA GAGGCCCAGT GGAAGAGGTG CTTTATGATA ACGCGGGCCT CTACGATAAC
    TTGCCATCGC CGACAATCTT TGCTCGCTCC TCTCCTGCTG ACCGGAGGGC ATCTCGGCCA
    CCCACTGACA GGCTGTCCTC TAACCACTAC AAATCCCCCA CCTCCTGCAG CCCACCCTGC
    TCCCCATCTG TCACTAACAC CTCTTCTGTG GGGAGGGCAT CTCTCGGGCT GCACTCCCAG
    TTGAAGAACA AAAAACCGCC AGCGTCATCT AATGGGGTTC CTGTAAAGGG GAAGGCTCCC
    AGCAGTCAGC AAAAAAAGGT GGAAACTGCA GGTGGTGTGA AACGGACAGC ATCAAATGCT
    GAGCAGTACA AATATGGGAA GAACCGGGTA GAAGCAGATG CCAAGCGGTT ACAGTCCAAA
    GAGGAAGAGC TGCTGAAGAG GAAAGAGGCC CTACGGAACA GGCTGGCACA GCTGCGGAAA
    GAGAGGAAGG ACCTGAGGGC CGCCATCGAA GTGAATGCAG GCAGGAAGAC CCAGGCAGCT
    CTGGAGGACA AGTTGAAGAG GCTGGAGGAA GAGTGCAAGC AGAGGGAGGC GGAGCGTGTC
    AGCCTGGAGC TGGAGCTGAC CGAGGTCAAG GAGAGCCTGA AGAAGGCCTT GGCGGGTGGC
    GTCACCCTAG GACTGGCCAT CGAGCCCAGG TCAGGGACAT CCAGTCCACA GTCTCCTGTG
    TTTCGGCATC GGACTCTGGA AAATTCTCCC ATCTCCAGCT GTGATACAAG CGATGCTGAG
    GGGCCTTTGC CTGTGAACAG TGCAGCCGTC CTGAAGAAGA GCCAGCCGTC CTCAGGCAGC
    TCCCCTTGCC GTGGCCACGT GCTGCAGAAG GCCAAGGAGT GGGAGCTGAA GAATGGGACA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006504161.1
    CDS226..2688
    Translation

    Target ORF information:

    RefSeq Version XM_006504098.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006504098.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAGAGT TAATAGTTGA ACTCCGCCTC TTTCTGGAGC TCCTGGACCA CGAGTACCTC 
    ACATCAACTG TCAGAGAGAA AAAGGCTGTG CTCACCAACA TCCTGCTGCG CCTGCAGTCA
    TCCAAGGGCT TTGAAGTGAA GGACCATGCT CAGAAGGCAG AGGCCAACAA CCTGCCGGCA
    CCACCCCAGA TGCCTCTGCC GGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGCGGGCCT
    CCGCCTTTAC CAACGTCCTC TCTCCCAGAG GGTTACTATG AGGAAGCCGT GCCGCTGAGC
    CCTGGGAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGATGCCAT GAGCAGTTCC
    TATGAGTCAT ATGATGAAGA GGAAGAGGAC GGGAAGGGGA AGAAAACTCG GCATCAGTGG
    CCGTCAGAGG AGGCCTCTAT GGACCTGGTG AAAGACGCCA AGATCTGTGC CTTCCTGCTG
    CGCAAGAAAC GCTTTGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG
    CTGTGCTATA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCGCT GCAGGGCTGC
    AGTATCACAT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG
    CAAGGCACAG ACCCTCTGGT TCTAGCTGTT CAGAGCAAGG AACAGGCTGA GCAGTGGTTG
    AAGGTAATCA AAGAAGCCTA CAGTGGCTGT AGCGGGCCCG TGGATCCGGA ATGTTCTCCA
    CCACCCAGCA CCAGCGCCCC CGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG
    AGGCCCAGCT CTGATGGAGA AGGTGGTGTG GAAAACGGAG TCACCACGTG TAATGGAAAA
    GAACAAGCAA AGAGGAAGAA ACCTTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC
    ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG
    CAGACATCAT CAGCAGAGGA GGATGTGCCC ACTTGTGGCT ACCTGAATGT GCTCTCTAAC
    AGCCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AACTCATTCT CCACAAGGAC
    AGGGCTGACC TGAAGACCCA CCTCGTCTCC ATCCCCCTCC GTGGCTGCGA GGTGATCCCG
    GGCCTGGATT CTAAGCACCC GTTGACCTTC CGGCTGCTTC GAAACGGACA GGAGGTGGCT
    GTTCTAGAGG CATCATCGTC TGAAGACATG GGCAGGTGGA TTGGCATCTT GCTGGCTGAG
    ACGGGGTCCT CCACGGACCC AGGAGCGCTG CACTACGACT ACATCGATGT GGAGATGTCA
    GCAAATGTCA TCCAGACGGC GAAACAGACC TTCTGCTTTA TGAACAGGCG GGCAGTGTCC
    ACTAGCCCAT ACCTGGGGAG CCTCTCCAAC GGCTACGCCC ATCCCAGTGG GACAGCACTA
    CACTATGATG ACGTCCCCTG TGTCAACGGC TCGTGGGACA TGGATGGCGG CTTTCCTGGC
    TGTGTGAGCA GAGGCCCAGT GGAAGAGGTG CTTTATGATA ACGCGGGCCT CTACGATAAC
    TTGCCATCGC CGACAATCTT TGCTCGCTCC TCTCCTGCTG ACCGGAGGGC ATCTCGGCCA
    CCCACTGACA GGCTGTCCTC TAACCACTAC AAATCCCCCA CCTCCTGCAG CCCACCCTGC
    TCCCCATCTG TCACTAACAC CTCTTCTGTG GGGAGGGCAT CTCTCGGGCT GCACTCCCAG
    TTGAAGAACA AAAAACCGCC AGCGTCATCT AATGGGGTTC CTGTAAAGGG GAAGGCTCCC
    AGCAGTCAGC AAAAAAAGGT GGAAACTGCA GGTGGTGTGA AACGGACAGC ATCAAATGCT
    GAGCAGTACA AATATGGGAA GAACCGGGTA GAAGCAGATG CCAAGCGGTT ACAGTCCAAA
    GAGGAAGAGC TGCTGAAGAG GAAAGAGGCC CTACGGAACA GGCTGGCACA GCTGCGGAAA
    GAGAGGAAGG ACCTGAGGGC CGCCATCGAA GTGAATGCAG GCAGGAAGAC CCAGGCAGCT
    CTGGAGGACA AGTTGAAGAG GCTGGAGGAA GAGTGCAAGC AGAGGGAGGC GGAGCGTGTC
    AGCCTGGAGC TGGAGCTGAC CGAGGTCAAG GAGAGCCTGA AGAAGGCCTT GGCGGGTGGC
    GTCACCCTAG GACTGGCCAT CGAGCCCAGG TCAGGGACAT CCAGTCCACA GTCTCCTGTG
    TTTCGGCATC GGACTCTGGA AAATTCTCCC ATCTCCAGCT GTGATACAAG CGATGCTGAG
    GGGCCTTTGC CTGTGAACAG TGCAGCCGTC CTGAAGAAGA GCCAGCCGTC CTCAGGCAGC
    TCCCCTTGCC GTGGCCACGT GCTGCAGAAG GCCAAGGAGT GGGAGCTGAA GAATGGGACA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35188
    Clone ID Related Accession (Same CDS sequence) XM_030254799.1 , XM_006504100.3
    Accession Version XM_006504100.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2139bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product actin filament-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTGATT ATGACTCTGA TGCCATGAGC AGTTCCTATG AGTCATATGA TGAAGAGGAA 
    GAGGACGGGA AGGGGAAGAA AACTCGGCAT CAGTGGCCGT CAGAGGAGGC CTCTATGGAC
    CTGGTGAAAG ACGCCAAGAT CTGTGCCTTC CTGCTGCGCA AGAAACGCTT TGGCCAGTGG
    ACCAAGCTGC TCTGCGTCAT CAAAGACACC AAGCTGCTGT GCTATAAAAG TTCCAAGGAC
    CAGCAGCCTC AGATGGAGCT GCCGCTGCAG GGCTGCAGTA TCACATACAT CCCGAGAGAC
    AGCAAGAAGA AGAAACACGA GCTGAAAATC ACCCAGCAAG GCACAGACCC TCTGGTTCTA
    GCTGTTCAGA GCAAGGAACA GGCTGAGCAG TGGTTGAAGG TAATCAAAGA AGCCTACAGT
    GGCTGTAGCG GGCCCGTGGA TCCGGAATGT TCTCCACCAC CCAGCACCAG CGCCCCCGTG
    AACAAGGCAG AACTGGAGAA GAAATTGTCT TCAGAGAGGC CCAGCTCTGA TGGAGAAGGT
    GGTGTGGAAA ACGGAGTCAC CACGTGTAAT GGAAAAGAAC AAGCAAAGAG GAAGAAACCT
    TCCAAGTCAG AGGCCAAGGG GACTGTGTCC AAGGTCACTG GGAAGAAAAT TACCAAGATC
    ATCGGTCTGG GAAAGAAGAA GCCATCCACA GATGAGCAGA CATCATCAGC AGAGGAGGAT
    GTGCCCACTT GTGGCTACCT GAATGTGCTC TCTAACAGCC GCTGGCGGGA GCGCTGGTGC
    AGAGTGAAGG ACAGCAAACT CATTCTCCAC AAGGACAGGG CTGACCTGAA GACCCACCTC
    GTCTCCATCC CCCTCCGTGG CTGCGAGGTG ATCCCGGGCC TGGATTCTAA GCACCCGTTG
    ACCTTCCGGC TGCTTCGAAA CGGACAGGAG GTGGCTGTTC TAGAGGCATC ATCGTCTGAA
    GACATGGGCA GGTGGATTGG CATCTTGCTG GCTGAGACGG GGTCCTCCAC GGACCCAGGA
    GCGCTGCACT ACGACTACAT CGATGTGGAG ATGTCAGCAA ATGTCATCCA GACGGCGAAA
    CAGACCTTCT GCTTTATGAA CAGGCGGGCA GTGTCCACTA GCCCATACCT GGGGAGCCTC
    TCCAACGGCT ACGCCCATCC CAGTGGGACA GCACTACACT ATGATGACGT CCCCTGTGTC
    AACGGCTCGT GGGACATGGA TGGCGGCTTT CCTGGCTGTG TGAGCAGAGG CCCAGTGGAA
    GAGGTGCTTT ATGATAACGC GGGCCTCTAC GATAACTTGC CATCGCCGAC AATCTTTGCT
    CGCTCCTCTC CTGCTGACCG GAGGGCATCT CGGCCACCCA CTGACAGGCT GTCCTCTAAC
    CACTACAAAT CCCCCACCTC CTGCAGCCCA CCCTGCTCCC CATCTGTCAC TAACACCTCT
    TCTGTGGGGA GGGCATCTCT CGGGCTGCAC TCCCAGTTGA AGAACAAAAA ACCGCCAGCG
    TCATCTAATG GGGTTCCTGT AAAGGGGAAG GCTCCCAGCA GTCAGCAAAA AAAGGTGGAA
    ACTGCAGGTG GTGTGAAACG GACAGCATCA AATGCTGAGC AGTACAAATA TGGGAAGAAC
    CGGGTAGAAG CAGATGCCAA GCGGTTACAG TCCAAAGAGG AAGAGCTGCT GAAGAGGAAA
    GAGGCCCTAC GGAACAGGCT GGCACAGCTG CGGAAAGAGA GGAAGGACCT GAGGGCCGCC
    ATCGAAGTGA ATGCAGGCAG GAAGACCCAG GCAGCTCTGG AGGACAAGTT GAAGAGGCTG
    GAGGAAGAGT GCAAGCAGAG GGAGGCGGAG CGTGTCAGCC TGGAGCTGGA GCTGACCGAG
    GTCAAGGAGA GCCTGAAGAA GGCCTTGGCG GGTGGCGTCA CCCTAGGACT GGCCATCGAG
    CCCAGGTCAG GGACATCCAG TCCACAGTCT CCTGTGTTTC GGCATCGGAC TCTGGAAAAT
    TCTCCCATCT CCAGCTGTGA TACAAGCGAT GCTGAGGGGC CTTTGCCTGT GAACAGTGCA
    GCCGTCCTGA AGAAGAGCCA GCCGTCCTCA GGCAGCTCCC CTTGCCGTGG CCACGTGCTG
    CAGAAGGCCA AGGAGTGGGA GCTGAAGAAT GGGACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006504163.1
    CDS86..2224
    Translation

    Target ORF information:

    RefSeq Version XM_006504100.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006504100.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTGATT ATGACTCTGA TGCCATGAGC AGTTCCTATG AGTCATATGA TGAAGAGGAA 
    GAGGACGGGA AGGGGAAGAA AACTCGGCAT CAGTGGCCGT CAGAGGAGGC CTCTATGGAC
    CTGGTGAAAG ACGCCAAGAT CTGTGCCTTC CTGCTGCGCA AGAAACGCTT TGGCCAGTGG
    ACCAAGCTGC TCTGCGTCAT CAAAGACACC AAGCTGCTGT GCTATAAAAG TTCCAAGGAC
    CAGCAGCCTC AGATGGAGCT GCCGCTGCAG GGCTGCAGTA TCACATACAT CCCGAGAGAC
    AGCAAGAAGA AGAAACACGA GCTGAAAATC ACCCAGCAAG GCACAGACCC TCTGGTTCTA
    GCTGTTCAGA GCAAGGAACA GGCTGAGCAG TGGTTGAAGG TAATCAAAGA AGCCTACAGT
    GGCTGTAGCG GGCCCGTGGA TCCGGAATGT TCTCCACCAC CCAGCACCAG CGCCCCCGTG
    AACAAGGCAG AACTGGAGAA GAAATTGTCT TCAGAGAGGC CCAGCTCTGA TGGAGAAGGT
    GGTGTGGAAA ACGGAGTCAC CACGTGTAAT GGAAAAGAAC AAGCAAAGAG GAAGAAACCT
    TCCAAGTCAG AGGCCAAGGG GACTGTGTCC AAGGTCACTG GGAAGAAAAT TACCAAGATC
    ATCGGTCTGG GAAAGAAGAA GCCATCCACA GATGAGCAGA CATCATCAGC AGAGGAGGAT
    GTGCCCACTT GTGGCTACCT GAATGTGCTC TCTAACAGCC GCTGGCGGGA GCGCTGGTGC
    AGAGTGAAGG ACAGCAAACT CATTCTCCAC AAGGACAGGG CTGACCTGAA GACCCACCTC
    GTCTCCATCC CCCTCCGTGG CTGCGAGGTG ATCCCGGGCC TGGATTCTAA GCACCCGTTG
    ACCTTCCGGC TGCTTCGAAA CGGACAGGAG GTGGCTGTTC TAGAGGCATC ATCGTCTGAA
    GACATGGGCA GGTGGATTGG CATCTTGCTG GCTGAGACGG GGTCCTCCAC GGACCCAGGA
    GCGCTGCACT ACGACTACAT CGATGTGGAG ATGTCAGCAA ATGTCATCCA GACGGCGAAA
    CAGACCTTCT GCTTTATGAA CAGGCGGGCA GTGTCCACTA GCCCATACCT GGGGAGCCTC
    TCCAACGGCT ACGCCCATCC CAGTGGGACA GCACTACACT ATGATGACGT CCCCTGTGTC
    AACGGCTCGT GGGACATGGA TGGCGGCTTT CCTGGCTGTG TGAGCAGAGG CCCAGTGGAA
    GAGGTGCTTT ATGATAACGC GGGCCTCTAC GATAACTTGC CATCGCCGAC AATCTTTGCT
    CGCTCCTCTC CTGCTGACCG GAGGGCATCT CGGCCACCCA CTGACAGGCT GTCCTCTAAC
    CACTACAAAT CCCCCACCTC CTGCAGCCCA CCCTGCTCCC CATCTGTCAC TAACACCTCT
    TCTGTGGGGA GGGCATCTCT CGGGCTGCAC TCCCAGTTGA AGAACAAAAA ACCGCCAGCG
    TCATCTAATG GGGTTCCTGT AAAGGGGAAG GCTCCCAGCA GTCAGCAAAA AAAGGTGGAA
    ACTGCAGGTG GTGTGAAACG GACAGCATCA AATGCTGAGC AGTACAAATA TGGGAAGAAC
    CGGGTAGAAG CAGATGCCAA GCGGTTACAG TCCAAAGAGG AAGAGCTGCT GAAGAGGAAA
    GAGGCCCTAC GGAACAGGCT GGCACAGCTG CGGAAAGAGA GGAAGGACCT GAGGGCCGCC
    ATCGAAGTGA ATGCAGGCAG GAAGACCCAG GCAGCTCTGG AGGACAAGTT GAAGAGGCTG
    GAGGAAGAGT GCAAGCAGAG GGAGGCGGAG CGTGTCAGCC TGGAGCTGGA GCTGACCGAG
    GTCAAGGAGA GCCTGAAGAA GGCCTTGGCG GGTGGCGTCA CCCTAGGACT GGCCATCGAG
    CCCAGGTCAG GGACATCCAG TCCACAGTCT CCTGTGTTTC GGCATCGGAC TCTGGAAAAT
    TCTCCCATCT CCAGCTGTGA TACAAGCGAT GCTGAGGGGC CTTTGCCTGT GAACAGTGCA
    GCCGTCCTGA AGAAGAGCCA GCCGTCCTCA GGCAGCTCCC CTTGCCGTGG CCACGTGCTG
    CAGAAGGCCA AGGAGTGGGA GCTGAAGAAT GGGACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35186
    Clone ID Related Accession (Same CDS sequence) XM_006504098.2 , XM_006504098.3
    Accession Version XM_006504098.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product actin filament-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAGAGT TAATAGTTGA ACTCCGCCTC TTTCTGGAGC TCCTGGACCA CGAGTACCTC 
    ACATCAACTG TCAGAGAGAA AAAGGCTGTG CTCACCAACA TCCTGCTGCG CCTGCAGTCA
    TCCAAGGGCT TTGAAGTGAA GGACCATGCT CAGAAGGCAG AGGCCAACAA CCTGCCGGCA
    CCACCCCAGA TGCCTCTGCC GGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGCGGGCCT
    CCGCCTTTAC CAACGTCCTC TCTCCCAGAG GGTTACTATG AGGAAGCCGT GCCGCTGAGC
    CCTGGGAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGATGCCAT GAGCAGTTCC
    TATGAGTCAT ATGATGAAGA GGAAGAGGAC GGGAAGGGGA AGAAAACTCG GCATCAGTGG
    CCGTCAGAGG AGGCCTCTAT GGACCTGGTG AAAGACGCCA AGATCTGTGC CTTCCTGCTG
    CGCAAGAAAC GCTTTGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG
    CTGTGCTATA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCGCT GCAGGGCTGC
    AGTATCACAT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG
    CAAGGCACAG ACCCTCTGGT TCTAGCTGTT CAGAGCAAGG AACAGGCTGA GCAGTGGTTG
    AAGGTAATCA AAGAAGCCTA CAGTGGCTGT AGCGGGCCCG TGGATCCGGA ATGTTCTCCA
    CCACCCAGCA CCAGCGCCCC CGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG
    AGGCCCAGCT CTGATGGAGA AGGTGGTGTG GAAAACGGAG TCACCACGTG TAATGGAAAA
    GAACAAGCAA AGAGGAAGAA ACCTTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC
    ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG
    CAGACATCAT CAGCAGAGGA GGATGTGCCC ACTTGTGGCT ACCTGAATGT GCTCTCTAAC
    AGCCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AACTCATTCT CCACAAGGAC
    AGGGCTGACC TGAAGACCCA CCTCGTCTCC ATCCCCCTCC GTGGCTGCGA GGTGATCCCG
    GGCCTGGATT CTAAGCACCC GTTGACCTTC CGGCTGCTTC GAAACGGACA GGAGGTGGCT
    GTTCTAGAGG CATCATCGTC TGAAGACATG GGCAGGTGGA TTGGCATCTT GCTGGCTGAG
    ACGGGGTCCT CCACGGACCC AGGAGCGCTG CACTACGACT ACATCGATGT GGAGATGTCA
    GCAAATGTCA TCCAGACGGC GAAACAGACC TTCTGCTTTA TGAACAGGCG GGCAGTGTCC
    ACTAGCCCAT ACCTGGGGAG CCTCTCCAAC GGCTACGCCC ATCCCAGTGG GACAGCACTA
    CACTATGATG ACGTCCCCTG TGTCAACGGC TCGTGGGACA TGGATGGCGG CTTTCCTGGC
    TGTGTGAGCA GAGGCCCAGT GGAAGAGGTG CTTTATGATA ACGCGGGCCT CTACGATAAC
    TTGCCATCGC CGACAATCTT TGCTCGCTCC TCTCCTGCTG ACCGGAGGGC ATCTCGGCCA
    CCCACTGACA GGCTGTCCTC TAACCACTAC AAATCCCCCA CCTCCTGCAG CCCACCCTGC
    TCCCCATCTG TCACTAACAC CTCTTCTGTG GGGAGGGCAT CTCTCGGGCT GCACTCCCAG
    TTGAAGAACA AAAAACCGCC AGCGTCATCT AATGGGGTTC CTGTAAAGGG GAAGGCTCCC
    AGCAGTCAGC AAAAAAAGGT GGAAACTGCA GGTGGTGTGA AACGGACAGC ATCAAATGCT
    GAGCAGTACA AATATGGGAA GAACCGGGTA GAAGCAGATG CCAAGCGGTT ACAGTCCAAA
    GAGGAAGAGC TGCTGAAGAG GAAAGAGGCC CTACGGAACA GGCTGGCACA GCTGCGGAAA
    GAGAGGAAGG ACCTGAGGGC CGCCATCGAA GTGAATGCAG GCAGGAAGAC CCAGGCAGCT
    CTGGAGGACA AGTTGAAGAG GCTGGAGGAA GAGTGCAAGC AGAGGGAGGC GGAGCGTGTC
    AGCCTGGAGC TGGAGCTGAC CGAGGTCAAG GAGAGCCTGA AGAAGGCCTT GGCGGGTGGC
    GTCACCCTAG GACTGGCCAT CGAGCCCAGG TCAGGGACAT CCAGTCCACA GTCTCCTGTG
    TTTCGGCATC GGACTCTGGA AAATTCTCCC ATCTCCAGCT GTGATACAAG CGATGCTGAG
    GGGCCTTTGC CTGTGAACAG TGCAGCCGTC CTGAAGAAGA GCCAGCCGTC CTCAGGCAGC
    TCCCCTTGCC GTGGCCACGT GCTGCAGAAG GCCAAGGAGT GGGAGCTGAA GAATGGGACA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006504161.1
    CDS221..2683
    Translation

    Target ORF information:

    RefSeq Version XM_006504098.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006504098.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAGAGT TAATAGTTGA ACTCCGCCTC TTTCTGGAGC TCCTGGACCA CGAGTACCTC 
    ACATCAACTG TCAGAGAGAA AAAGGCTGTG CTCACCAACA TCCTGCTGCG CCTGCAGTCA
    TCCAAGGGCT TTGAAGTGAA GGACCATGCT CAGAAGGCAG AGGCCAACAA CCTGCCGGCA
    CCACCCCAGA TGCCTCTGCC GGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGCGGGCCT
    CCGCCTTTAC CAACGTCCTC TCTCCCAGAG GGTTACTATG AGGAAGCCGT GCCGCTGAGC
    CCTGGGAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGATGCCAT GAGCAGTTCC
    TATGAGTCAT ATGATGAAGA GGAAGAGGAC GGGAAGGGGA AGAAAACTCG GCATCAGTGG
    CCGTCAGAGG AGGCCTCTAT GGACCTGGTG AAAGACGCCA AGATCTGTGC CTTCCTGCTG
    CGCAAGAAAC GCTTTGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG
    CTGTGCTATA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCGCT GCAGGGCTGC
    AGTATCACAT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG
    CAAGGCACAG ACCCTCTGGT TCTAGCTGTT CAGAGCAAGG AACAGGCTGA GCAGTGGTTG
    AAGGTAATCA AAGAAGCCTA CAGTGGCTGT AGCGGGCCCG TGGATCCGGA ATGTTCTCCA
    CCACCCAGCA CCAGCGCCCC CGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG
    AGGCCCAGCT CTGATGGAGA AGGTGGTGTG GAAAACGGAG TCACCACGTG TAATGGAAAA
    GAACAAGCAA AGAGGAAGAA ACCTTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC
    ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG
    CAGACATCAT CAGCAGAGGA GGATGTGCCC ACTTGTGGCT ACCTGAATGT GCTCTCTAAC
    AGCCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AACTCATTCT CCACAAGGAC
    AGGGCTGACC TGAAGACCCA CCTCGTCTCC ATCCCCCTCC GTGGCTGCGA GGTGATCCCG
    GGCCTGGATT CTAAGCACCC GTTGACCTTC CGGCTGCTTC GAAACGGACA GGAGGTGGCT
    GTTCTAGAGG CATCATCGTC TGAAGACATG GGCAGGTGGA TTGGCATCTT GCTGGCTGAG
    ACGGGGTCCT CCACGGACCC AGGAGCGCTG CACTACGACT ACATCGATGT GGAGATGTCA
    GCAAATGTCA TCCAGACGGC GAAACAGACC TTCTGCTTTA TGAACAGGCG GGCAGTGTCC
    ACTAGCCCAT ACCTGGGGAG CCTCTCCAAC GGCTACGCCC ATCCCAGTGG GACAGCACTA
    CACTATGATG ACGTCCCCTG TGTCAACGGC TCGTGGGACA TGGATGGCGG CTTTCCTGGC
    TGTGTGAGCA GAGGCCCAGT GGAAGAGGTG CTTTATGATA ACGCGGGCCT CTACGATAAC
    TTGCCATCGC CGACAATCTT TGCTCGCTCC TCTCCTGCTG ACCGGAGGGC ATCTCGGCCA
    CCCACTGACA GGCTGTCCTC TAACCACTAC AAATCCCCCA CCTCCTGCAG CCCACCCTGC
    TCCCCATCTG TCACTAACAC CTCTTCTGTG GGGAGGGCAT CTCTCGGGCT GCACTCCCAG
    TTGAAGAACA AAAAACCGCC AGCGTCATCT AATGGGGTTC CTGTAAAGGG GAAGGCTCCC
    AGCAGTCAGC AAAAAAAGGT GGAAACTGCA GGTGGTGTGA AACGGACAGC ATCAAATGCT
    GAGCAGTACA AATATGGGAA GAACCGGGTA GAAGCAGATG CCAAGCGGTT ACAGTCCAAA
    GAGGAAGAGC TGCTGAAGAG GAAAGAGGCC CTACGGAACA GGCTGGCACA GCTGCGGAAA
    GAGAGGAAGG ACCTGAGGGC CGCCATCGAA GTGAATGCAG GCAGGAAGAC CCAGGCAGCT
    CTGGAGGACA AGTTGAAGAG GCTGGAGGAA GAGTGCAAGC AGAGGGAGGC GGAGCGTGTC
    AGCCTGGAGC TGGAGCTGAC CGAGGTCAAG GAGAGCCTGA AGAAGGCCTT GGCGGGTGGC
    GTCACCCTAG GACTGGCCAT CGAGCCCAGG TCAGGGACAT CCAGTCCACA GTCTCCTGTG
    TTTCGGCATC GGACTCTGGA AAATTCTCCC ATCTCCAGCT GTGATACAAG CGATGCTGAG
    GGGCCTTTGC CTGTGAACAG TGCAGCCGTC CTGAAGAAGA GCCAGCCGTC CTCAGGCAGC
    TCCCCTTGCC GTGGCCACGT GCTGCAGAAG GCCAAGGAGT GGGAGCTGAA GAATGGGACA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35188
    Clone ID Related Accession (Same CDS sequence) XM_030254799.1 , XM_006504100.3
    Accession Version XM_030254799.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2139bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product actin filament-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTGATT ATGACTCTGA TGCCATGAGC AGTTCCTATG AGTCATATGA TGAAGAGGAA 
    GAGGACGGGA AGGGGAAGAA AACTCGGCAT CAGTGGCCGT CAGAGGAGGC CTCTATGGAC
    CTGGTGAAAG ACGCCAAGAT CTGTGCCTTC CTGCTGCGCA AGAAACGCTT TGGCCAGTGG
    ACCAAGCTGC TCTGCGTCAT CAAAGACACC AAGCTGCTGT GCTATAAAAG TTCCAAGGAC
    CAGCAGCCTC AGATGGAGCT GCCGCTGCAG GGCTGCAGTA TCACATACAT CCCGAGAGAC
    AGCAAGAAGA AGAAACACGA GCTGAAAATC ACCCAGCAAG GCACAGACCC TCTGGTTCTA
    GCTGTTCAGA GCAAGGAACA GGCTGAGCAG TGGTTGAAGG TAATCAAAGA AGCCTACAGT
    GGCTGTAGCG GGCCCGTGGA TCCGGAATGT TCTCCACCAC CCAGCACCAG CGCCCCCGTG
    AACAAGGCAG AACTGGAGAA GAAATTGTCT TCAGAGAGGC CCAGCTCTGA TGGAGAAGGT
    GGTGTGGAAA ACGGAGTCAC CACGTGTAAT GGAAAAGAAC AAGCAAAGAG GAAGAAACCT
    TCCAAGTCAG AGGCCAAGGG GACTGTGTCC AAGGTCACTG GGAAGAAAAT TACCAAGATC
    ATCGGTCTGG GAAAGAAGAA GCCATCCACA GATGAGCAGA CATCATCAGC AGAGGAGGAT
    GTGCCCACTT GTGGCTACCT GAATGTGCTC TCTAACAGCC GCTGGCGGGA GCGCTGGTGC
    AGAGTGAAGG ACAGCAAACT CATTCTCCAC AAGGACAGGG CTGACCTGAA GACCCACCTC
    GTCTCCATCC CCCTCCGTGG CTGCGAGGTG ATCCCGGGCC TGGATTCTAA GCACCCGTTG
    ACCTTCCGGC TGCTTCGAAA CGGACAGGAG GTGGCTGTTC TAGAGGCATC ATCGTCTGAA
    GACATGGGCA GGTGGATTGG CATCTTGCTG GCTGAGACGG GGTCCTCCAC GGACCCAGGA
    GCGCTGCACT ACGACTACAT CGATGTGGAG ATGTCAGCAA ATGTCATCCA GACGGCGAAA
    CAGACCTTCT GCTTTATGAA CAGGCGGGCA GTGTCCACTA GCCCATACCT GGGGAGCCTC
    TCCAACGGCT ACGCCCATCC CAGTGGGACA GCACTACACT ATGATGACGT CCCCTGTGTC
    AACGGCTCGT GGGACATGGA TGGCGGCTTT CCTGGCTGTG TGAGCAGAGG CCCAGTGGAA
    GAGGTGCTTT ATGATAACGC GGGCCTCTAC GATAACTTGC CATCGCCGAC AATCTTTGCT
    CGCTCCTCTC CTGCTGACCG GAGGGCATCT CGGCCACCCA CTGACAGGCT GTCCTCTAAC
    CACTACAAAT CCCCCACCTC CTGCAGCCCA CCCTGCTCCC CATCTGTCAC TAACACCTCT
    TCTGTGGGGA GGGCATCTCT CGGGCTGCAC TCCCAGTTGA AGAACAAAAA ACCGCCAGCG
    TCATCTAATG GGGTTCCTGT AAAGGGGAAG GCTCCCAGCA GTCAGCAAAA AAAGGTGGAA
    ACTGCAGGTG GTGTGAAACG GACAGCATCA AATGCTGAGC AGTACAAATA TGGGAAGAAC
    CGGGTAGAAG CAGATGCCAA GCGGTTACAG TCCAAAGAGG AAGAGCTGCT GAAGAGGAAA
    GAGGCCCTAC GGAACAGGCT GGCACAGCTG CGGAAAGAGA GGAAGGACCT GAGGGCCGCC
    ATCGAAGTGA ATGCAGGCAG GAAGACCCAG GCAGCTCTGG AGGACAAGTT GAAGAGGCTG
    GAGGAAGAGT GCAAGCAGAG GGAGGCGGAG CGTGTCAGCC TGGAGCTGGA GCTGACCGAG
    GTCAAGGAGA GCCTGAAGAA GGCCTTGGCG GGTGGCGTCA CCCTAGGACT GGCCATCGAG
    CCCAGGTCAG GGACATCCAG TCCACAGTCT CCTGTGTTTC GGCATCGGAC TCTGGAAAAT
    TCTCCCATCT CCAGCTGTGA TACAAGCGAT GCTGAGGGGC CTTTGCCTGT GAACAGTGCA
    GCCGTCCTGA AGAAGAGCCA GCCGTCCTCA GGCAGCTCCC CTTGCCGTGG CCACGTGCTG
    CAGAAGGCCA AGGAGTGGGA GCTGAAGAAT GGGACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110659.1
    CDS437..2575
    Translation

    Target ORF information:

    RefSeq Version XM_030254799.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254799.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTGATT ATGACTCTGA TGCCATGAGC AGTTCCTATG AGTCATATGA TGAAGAGGAA 
    GAGGACGGGA AGGGGAAGAA AACTCGGCAT CAGTGGCCGT CAGAGGAGGC CTCTATGGAC
    CTGGTGAAAG ACGCCAAGAT CTGTGCCTTC CTGCTGCGCA AGAAACGCTT TGGCCAGTGG
    ACCAAGCTGC TCTGCGTCAT CAAAGACACC AAGCTGCTGT GCTATAAAAG TTCCAAGGAC
    CAGCAGCCTC AGATGGAGCT GCCGCTGCAG GGCTGCAGTA TCACATACAT CCCGAGAGAC
    AGCAAGAAGA AGAAACACGA GCTGAAAATC ACCCAGCAAG GCACAGACCC TCTGGTTCTA
    GCTGTTCAGA GCAAGGAACA GGCTGAGCAG TGGTTGAAGG TAATCAAAGA AGCCTACAGT
    GGCTGTAGCG GGCCCGTGGA TCCGGAATGT TCTCCACCAC CCAGCACCAG CGCCCCCGTG
    AACAAGGCAG AACTGGAGAA GAAATTGTCT TCAGAGAGGC CCAGCTCTGA TGGAGAAGGT
    GGTGTGGAAA ACGGAGTCAC CACGTGTAAT GGAAAAGAAC AAGCAAAGAG GAAGAAACCT
    TCCAAGTCAG AGGCCAAGGG GACTGTGTCC AAGGTCACTG GGAAGAAAAT TACCAAGATC
    ATCGGTCTGG GAAAGAAGAA GCCATCCACA GATGAGCAGA CATCATCAGC AGAGGAGGAT
    GTGCCCACTT GTGGCTACCT GAATGTGCTC TCTAACAGCC GCTGGCGGGA GCGCTGGTGC
    AGAGTGAAGG ACAGCAAACT CATTCTCCAC AAGGACAGGG CTGACCTGAA GACCCACCTC
    GTCTCCATCC CCCTCCGTGG CTGCGAGGTG ATCCCGGGCC TGGATTCTAA GCACCCGTTG
    ACCTTCCGGC TGCTTCGAAA CGGACAGGAG GTGGCTGTTC TAGAGGCATC ATCGTCTGAA
    GACATGGGCA GGTGGATTGG CATCTTGCTG GCTGAGACGG GGTCCTCCAC GGACCCAGGA
    GCGCTGCACT ACGACTACAT CGATGTGGAG ATGTCAGCAA ATGTCATCCA GACGGCGAAA
    CAGACCTTCT GCTTTATGAA CAGGCGGGCA GTGTCCACTA GCCCATACCT GGGGAGCCTC
    TCCAACGGCT ACGCCCATCC CAGTGGGACA GCACTACACT ATGATGACGT CCCCTGTGTC
    AACGGCTCGT GGGACATGGA TGGCGGCTTT CCTGGCTGTG TGAGCAGAGG CCCAGTGGAA
    GAGGTGCTTT ATGATAACGC GGGCCTCTAC GATAACTTGC CATCGCCGAC AATCTTTGCT
    CGCTCCTCTC CTGCTGACCG GAGGGCATCT CGGCCACCCA CTGACAGGCT GTCCTCTAAC
    CACTACAAAT CCCCCACCTC CTGCAGCCCA CCCTGCTCCC CATCTGTCAC TAACACCTCT
    TCTGTGGGGA GGGCATCTCT CGGGCTGCAC TCCCAGTTGA AGAACAAAAA ACCGCCAGCG
    TCATCTAATG GGGTTCCTGT AAAGGGGAAG GCTCCCAGCA GTCAGCAAAA AAAGGTGGAA
    ACTGCAGGTG GTGTGAAACG GACAGCATCA AATGCTGAGC AGTACAAATA TGGGAAGAAC
    CGGGTAGAAG CAGATGCCAA GCGGTTACAG TCCAAAGAGG AAGAGCTGCT GAAGAGGAAA
    GAGGCCCTAC GGAACAGGCT GGCACAGCTG CGGAAAGAGA GGAAGGACCT GAGGGCCGCC
    ATCGAAGTGA ATGCAGGCAG GAAGACCCAG GCAGCTCTGG AGGACAAGTT GAAGAGGCTG
    GAGGAAGAGT GCAAGCAGAG GGAGGCGGAG CGTGTCAGCC TGGAGCTGGA GCTGACCGAG
    GTCAAGGAGA GCCTGAAGAA GGCCTTGGCG GGTGGCGTCA CCCTAGGACT GGCCATCGAG
    CCCAGGTCAG GGACATCCAG TCCACAGTCT CCTGTGTTTC GGCATCGGAC TCTGGAAAAT
    TCTCCCATCT CCAGCTGTGA TACAAGCGAT GCTGAGGGGC CTTTGCCTGT GAACAGTGCA
    GCCGTCCTGA AGAAGAGCCA GCCGTCCTCA GGCAGCTCCC CTTGCCGTGG CCACGTGCTG
    CAGAAGGCCA AGGAGTGGGA GCTGAAGAAT GGGACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu89013
    Clone ID Related Accession (Same CDS sequence) XM_030254800.1
    Accession Version XM_030254800.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product actin filament-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCTGATT ATGACTCTGA TGCCATGAGC AGTTCCTATG AGTCATATGA TGAAGAGGAA 
    GAGGACGGGA AGGGGAAGAA AACTCGGCAT CAGTGGCCGT CAGAGGAGGC CTCTATGGAC
    CTGGTGAAAG ACGCCAAGAT CTGTGCCTTC CTGCTGCGCA AGAAACGCTT TGGCCAGTGG
    ACCAAGCTGC TCTGCGTCAT CAAAGACACC AAGCTGCTGT GCTATAAAAG TTCCAAGGAC
    CAGCAGCCTC AGATGGAGCT GCCGCTGCAG GGCTGCAGTA TCACATACAT CCCGAGAGAC
    AGCAAGAAGA AGAAACACGA GCTGAAAATC ACCCAGCAAG GCACAGACCC TCTGGTTCTA
    GCTGTTCAGA GCAAGGAACA GGCTGAGCAG TGGTTGAAGG TAATCAAAGA AGCCTACAGT
    GGCTGTAGCG GGCCCGTGGA TCCGGAATGT TCTCCACCAC CCAGCACCAG CGCCCCCGTG
    AACAAGGCAG AACTGGAGAA GAAATTGTCT TCAGAGAGGC CCAGCTCTGA TGGAGAAGGT
    GGTGTGGAAA ACGGAGTCAC CACGTGTAAT GGAAAAGAAC AAGCAAAGAG GAAGAAACCT
    TCCAAGTCAG AGGCCAAGGG GACTGTGTCC AAGGTCACTG GGAAGAAAAT TACCAAGATC
    ATCGGTCTGG GAAAGAAGAA GCCATCCACA GATGAGCAGA CATCATCAGC AGAGGAGGAT
    GTGCCCACTT GTGGCTACCT GAATGTGCTC TCTAACAGCC GCTGGCGGGA GCGCTGGTGC
    AGAGTGAAGG ACAGCAAACT CATTCTCCAC AAGGACAGGG CTGACCTGAA GACCCACCTC
    GTCTCCATCC CCCTCCGTGG CTGCGAGGTG ATCCCGGGCC TGGATTCTAA GCACCCGTTG
    ACCTTCCGGC TGCTTCGAAA CGGACAGGAG GTGGCTGTTC TAGAGGCATC ATCGTCTGAA
    GACATGGGCA GGTGGATTGG CATCTTGCTG GCTGAGACGG GGTCCTCCAC GGACCCAGGA
    GCGCTGCACT ACGACTACAT CGATGTGGAG ATGTCAGCAA ATGTCATCCA GACGGCGAAA
    CAGACCTTCT GCTTTATGAA CAGGCGGGCA GTGTCCACTA GCCCATACCT GGGGAGCCTC
    TCCAACGGCT ACGCCCATCC CAGTGGGACA GCACTACACT ATGATGACGT CCCCTGTGTC
    AACGGCTCGT TGAAGAACAA AAAACCGCCA GCGTCATCTA ATGGGGTTCC TGTAAAGGGG
    AAGGCTCCCA GCAGTCAGCA AAAAAAGGTG GAAACTGCAG GTGGTGTGAA ACGGACAGCA
    TCAAATGCTG AGCAGTACAA ATATGGGAAG AACCGGGTAG AAGCAGATGC CAAGCGGTTA
    CAGTCCAAAG AGGAAGAGCT GCTGAAGAGG AAAGAGGCCC TACGGAACAG GCTGGCACAG
    CTGCGGAAAG AGAGGAAGGA CCTGAGGGCC GCCATCGAAG TGAATGCAGG CAGGAAGACC
    CAGGCAGCTC TGGAGGACAA GTTGAAGAGG CTGGAGGAAG AGTGCAAGCA GAGGGAGGCG
    GAGCGTGTCA GCCTGGAGCT GGAGCTGACC GAGGTCAAGG AGAGCCTGAA GAAGGCCTTG
    GCGGGTGGCG TCACCCTAGG ACTGGCCATC GAGCCCAGGT CAGGGACATC CAGTCCACAG
    TCTCCTGTGT TTCGGCATCG GACTCTGGAA AATTCTCCCA TCTCCAGCTG TGATACAAGC
    GATGCTGAGG GGCCTTTGCC TGTGAACAGT GCAGCCGTCC TGAAGAAGAG CCAGCCGTCC
    TCAGGCAGCT CCCCTTGCCG TGGCCACGTG CTGCAGAAGG CCAAGGAGTG GGAGCTGAAG
    AATGGGACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110660.1
    CDS436..2307
    Translation

    Target ORF information:

    RefSeq Version XM_030254800.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus actin filament associated protein 1 (Afap1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254800.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCTGATT ATGACTCTGA TGCCATGAGC AGTTCCTATG AGTCATATGA TGAAGAGGAA 
    GAGGACGGGA AGGGGAAGAA AACTCGGCAT CAGTGGCCGT CAGAGGAGGC CTCTATGGAC
    CTGGTGAAAG ACGCCAAGAT CTGTGCCTTC CTGCTGCGCA AGAAACGCTT TGGCCAGTGG
    ACCAAGCTGC TCTGCGTCAT CAAAGACACC AAGCTGCTGT GCTATAAAAG TTCCAAGGAC
    CAGCAGCCTC AGATGGAGCT GCCGCTGCAG GGCTGCAGTA TCACATACAT CCCGAGAGAC
    AGCAAGAAGA AGAAACACGA GCTGAAAATC ACCCAGCAAG GCACAGACCC TCTGGTTCTA
    GCTGTTCAGA GCAAGGAACA GGCTGAGCAG TGGTTGAAGG TAATCAAAGA AGCCTACAGT
    GGCTGTAGCG GGCCCGTGGA TCCGGAATGT TCTCCACCAC CCAGCACCAG CGCCCCCGTG
    AACAAGGCAG AACTGGAGAA GAAATTGTCT TCAGAGAGGC CCAGCTCTGA TGGAGAAGGT
    GGTGTGGAAA ACGGAGTCAC CACGTGTAAT GGAAAAGAAC AAGCAAAGAG GAAGAAACCT
    TCCAAGTCAG AGGCCAAGGG GACTGTGTCC AAGGTCACTG GGAAGAAAAT TACCAAGATC
    ATCGGTCTGG GAAAGAAGAA GCCATCCACA GATGAGCAGA CATCATCAGC AGAGGAGGAT
    GTGCCCACTT GTGGCTACCT GAATGTGCTC TCTAACAGCC GCTGGCGGGA GCGCTGGTGC
    AGAGTGAAGG ACAGCAAACT CATTCTCCAC AAGGACAGGG CTGACCTGAA GACCCACCTC
    GTCTCCATCC CCCTCCGTGG CTGCGAGGTG ATCCCGGGCC TGGATTCTAA GCACCCGTTG
    ACCTTCCGGC TGCTTCGAAA CGGACAGGAG GTGGCTGTTC TAGAGGCATC ATCGTCTGAA
    GACATGGGCA GGTGGATTGG CATCTTGCTG GCTGAGACGG GGTCCTCCAC GGACCCAGGA
    GCGCTGCACT ACGACTACAT CGATGTGGAG ATGTCAGCAA ATGTCATCCA GACGGCGAAA
    CAGACCTTCT GCTTTATGAA CAGGCGGGCA GTGTCCACTA GCCCATACCT GGGGAGCCTC
    TCCAACGGCT ACGCCCATCC CAGTGGGACA GCACTACACT ATGATGACGT CCCCTGTGTC
    AACGGCTCGT TGAAGAACAA AAAACCGCCA GCGTCATCTA ATGGGGTTCC TGTAAAGGGG
    AAGGCTCCCA GCAGTCAGCA AAAAAAGGTG GAAACTGCAG GTGGTGTGAA ACGGACAGCA
    TCAAATGCTG AGCAGTACAA ATATGGGAAG AACCGGGTAG AAGCAGATGC CAAGCGGTTA
    CAGTCCAAAG AGGAAGAGCT GCTGAAGAGG AAAGAGGCCC TACGGAACAG GCTGGCACAG
    CTGCGGAAAG AGAGGAAGGA CCTGAGGGCC GCCATCGAAG TGAATGCAGG CAGGAAGACC
    CAGGCAGCTC TGGAGGACAA GTTGAAGAGG CTGGAGGAAG AGTGCAAGCA GAGGGAGGCG
    GAGCGTGTCA GCCTGGAGCT GGAGCTGACC GAGGTCAAGG AGAGCCTGAA GAAGGCCTTG
    GCGGGTGGCG TCACCCTAGG ACTGGCCATC GAGCCCAGGT CAGGGACATC CAGTCCACAG
    TCTCCTGTGT TTCGGCATCG GACTCTGGAA AATTCTCCCA TCTCCAGCTG TGATACAAGC
    GATGCTGAGG GGCCTTTGCC TGTGAACAGT GCAGCCGTCC TGAAGAAGAG CCAGCCGTCC
    TCAGGCAGCT CCCCTTGCCG TGGCCACGTG CTGCAGAAGG CCAAGGAGTG GGAGCTGAAG
    AATGGGACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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