Epn3 cDNA ORF clone, Mus musculus(house mouse)
Gene Symbol | Epn3 |
Entrez Gene ID | 71889 |
Full Name | epsin 3 |
Synonyms | 2310022G12Rik |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
mRNA and Protein(s)
mRNA | Protein | Name |
---|---|---|
NM_001361558.1 | NP_001348487.1 | epsin-3 isoform 2 |
XM_006534266.2 | XP_006534329.1 | epsin-3 isoform X1 |
NM_027984.3 | NP_082260.1 | epsin-3 isoform 1 |
XM_006534267.2 | XP_006534330.1 | epsin-3 isoform X2 |
XM_006534266.3 | XP_006534329.1 | epsin-3 isoform X1 |
XM_006534266.4 | XP_006534329.1 | epsin-3 isoform X1 |

Mus musculus (house mouse) | Epn3 | NP_082260.1 |
Gallus gallus (chicken) | EPN3 | XP_003642413.2 |
Canis lupus familiaris (dog) | EPN3 | XP_005624698.1 |
Rattus norvegicus (Norway rat) | Epn3 | NP_001019962.1 |
Pan troglodytes (chimpanzee) | EPN3 | XP_003315664.1 |
Bos taurus (cattle) | EPN3 | NP_001039899.1 |
Danio rerio (zebrafish) | LOC794862 | XP_005164437.1 |
Homo sapiens (human) | EPN3 | NP_060427.2 |
Danio rerio (zebrafish) | LOC571453 | XP_700135.5 |
Xenopus tropicalis (tropical clawed frog) | epn3 | NP_001005693.1 |

Related articles in PubMed
EPSIN 3, A Novel p53 Target, Regulates the Apoptotic Pathway and Gastric Carcinogenesis.
Mori J, Tanikawa C, Ohnishi N, Funauchi Y, Toyoshima O, Ueda K, Matsuda K
Neoplasia (New York, N.Y.)19(3)185-195(2017 Mar)
High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)
Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits.
Messa M, Fern?ndez-Busnadiego R, Sun EW, Chen H, Czapla H, Wrasman K, Wu Y, Ko G, Ross T, Wendland B, De Camilli P
eLife3e03311(2014 Aug)
The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Selective high-level expression of epsin 3 in gastric parietal cells, where it is localized at endocytic sites of apical canaliculi.
Ko G, Paradise S, Chen H, Graham M, Vecchi M, Bianchi F, Cremona O, Di Fiore PP, De Camilli P
Proceedings of the National Academy of Sciences of the United States of America107(50)21511-6(2010 Dec)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits.
Title: Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits.
data suggest that epsin 3 cooperates with other bilayer binding proteins with curvature sensing/generating properties in the specialized traffic and membrane remodeling processes typical of gastric parietal cells
Title: Selective high-level expression of epsin 3 in gastric parietal cells, where it is localized at endocytic sites of apical canaliculi.
The following Epn3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Epn3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OMu46961 | |
Clone ID Related Accession (Same CDS sequence) | NM_001361558.1 , XM_006534267.2 | |
Accession Version | NM_001361558.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-09-29 | |
Organism | Mus musculus(house mouse) | |
Product | epsin-3 isoform 2 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645809.8. On Mar 29, 2018 this sequence version replaced XM_006534267.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK158031.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001348487.1 |
CDS | 422..2251 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu46961 | |
Clone ID Related Accession (Same CDS sequence) | NM_001361558.1 , XM_006534267.2 | |
Accession Version | XM_006534267.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | epsin-3 isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534267.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006534330.1 |
CDS | 417..2246 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu04899 | |
Clone ID Related Accession (Same CDS sequence) | XM_006534266.2 , NM_027984.3 , XM_006534266.3 , XM_006534266.4 | |
Accession Version | NM_027984.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-02-22 | |
Organism | Mus musculus(house mouse) | |
Product | epsin-3 isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645809.8 and AK009469.1. On May 1, 2010 this sequence version replaced NM_027984.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC016454.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_082260.1 |
CDS | 422..2332 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu04899 | |
Clone ID Related Accession (Same CDS sequence) | XM_006534266.2 , NM_027984.3 , XM_006534266.3 , XM_006534266.4 | |
Accession Version | XM_006534266.2 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-02-08 | |
Organism | Mus musculus(House mouse) | |
Product | epsin-3 isoform X1 | |
Comment |
1 | ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006534329.1 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu04899 | |
Clone ID Related Accession (Same CDS sequence) | XM_006534266.2 , NM_027984.3 , XM_006534266.3 , XM_006534266.4 | |
Accession Version | XM_006534266.3 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | epsin-3 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534266.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006534329.1 |
CDS | 519..2429 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu04899 | |
Clone ID Related Accession (Same CDS sequence) | XM_006534266.2 , NM_027984.3 , XM_006534266.3 , XM_006534266.4 | |
Accession Version | XM_006534266.4 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | epsin-3 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534266.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006534329.1 |
CDS | 229..2139 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
![]() |
EPSIN 3, A Novel p53 Target, Regulates the Apoptotic Pathway and Gastric Carcinogenesis. |
![]() |
High-throughput discovery of novel developmental phenotypes. |
![]() |
Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits. |
![]() |
The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data. |
![]() |
Selective high-level expression of epsin 3 in gastric parietal cells, where it is localized at endocytic sites of apical canaliculi. |