Gene Symbol | Sun5 |
Entrez Gene ID | 76407 |
Full Name | Sad1 and UNC84 domain containing 5 |
Synonyms | 1700021O15Rik,SPAG4L-2,SRG4,Spag4l |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
ORF » Species Summary » Mus musculus » Sun5 cDNA ORF clone
gRNAs
Gene Symbol | Sun5 |
Entrez Gene ID | 76407 |
Full Name | Sad1 and UNC84 domain containing 5 |
Synonyms | 1700021O15Rik,SPAG4L-2,SRG4,Spag4l |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
mRNA | Protein | Name |
---|---|---|
XM_006500372.2 | XP_006500435.2 | SUN domain-containing protein 5 isoform X1 |
XM_030252189.1 | XP_030108049.1 | SUN domain-containing protein 5 isoform X3 |
XM_006500372.1 | XP_006500435.1 | SUN domain-containing protein 5 isoform X1 |
NM_029599.2 | NP_083875.1 | SUN domain-containing protein 5 isoform 2 |
NM_001305048.1 | NP_001291977.1 | SUN domain-containing protein 5 isoform 1 |
XM_006500374.1 | XP_006500437.1 | SUN domain-containing protein 5 isoform X2 |
XM_006500375.2 | XP_006500438.1 | SUN domain-containing protein 5 isoform X4 |
XM_011239838.1 | XP_011238140.1 | SUN domain-containing protein 5 isoform X5 |
Mechanistic insights into acephalic spermatozoa syndrome-associated mutations in the human SUN5 gene.
Shang Y, Yan J, Tang W, Liu C, Xiao S, Guo Y, Yuan L, Chen L, Jiang H, Guo X, Qiao J, Li W
The Journal of biological chemistry(2018 Jan)
Essential role for SUN5 in anchoring sperm head to the tail.
Shang Y, Zhu F, Wang L, Ouyang YC, Dong MZ, Liu C, Zhao H, Cui X, Ma D, Zhang Z, Yang X, Guo Y, Liu F, Yuan L, Gao F, Guo X, Sun QY, Cao Y, Li W
eLife6(2017 Sep)
Dynamics of Sun5 localization during spermatogenesis in wild type and Dpy19l2 knock-out mice indicates that Sun5 is not involved in acrosome attachment to the nuclear envelope.
Yassine S, Escoffier J, Abi Nahed R, Nahed RA, Pierre V, Karaouzene T, Ray PF, Arnoult C
PloS one10(3)e0118698(2015)
SPAG4L, a novel nuclear envelope protein involved in the meiotic stage of spermatogenesis.
Jiang XZ, Yang MG, Huang LH, Li CQ, Xing XW
DNA and cell biology30(11)875-82(2011 Nov)
SPAG4L/SPAG4L-2 are testis-specific SUN domain proteins restricted to the apical nuclear envelope of round spermatids facing the acrosome.
Frohnert C, Schweizer S, Hoyer-Fender S
Molecular human reproduction17(4)207-18(2011 Apr)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
In Dpy19l2 knock-out round spermatids, the detachment of the acrosome leads to Sun5 and lamin B1 relocalization, confirming that Sun5 protein is excluded from the nuclear envelope facing the acrosome.
Title: Dynamics of Sun5 localization during spermatogenesis in wild type and Dpy19l2 knock-out mice indicates that Sun5 is not involved in acrosome attachment to the nuclear envelope.
SPAG4L may play an important role in the meiotic stage of spermatogenesis
Title: SPAG4L, a novel nuclear envelope protein involved in the meiotic stage of spermatogenesis.
SPAG4L-2 and SPAG4L are transmembrane proteins that localize to the nuclear envelope.
Title: SPAG4L/SPAG4L-2 are testis-specific SUN domain proteins restricted to the apical nuclear envelope of round spermatids facing the acrosome.
SRG4 was cloned into pUCm-T vectors and sequenced, subcloned into a prokaryotic expression vector and expression of induced by IPTG.
Title: [Prokaryotic expression and purification of SRG4, a novel mouse spermatogenesis gene].
Results indicated that the expression of SRG4 was regulated by development, and SRG4 was mainly expressed in the cytoplasm of spermatocytes and round spermatids.
Title: [Expression of a novel spermatogenesis gene SRG4 in postpartum normal and cryptorchid mouse testis].
The following Sun5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sun5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OMu07191 | |
Clone ID Related Accession (Same CDS sequence) | NM_029599.2 | |
Accession Version | NM_029599.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP |
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Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-11-16 | |
Organism | Mus musculus(house mouse) | |
Product | SUN domain-containing protein 5 isoform 2 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY307077.1 and AL833803.8. On Feb 19, 2015 this sequence version replaced NM_029599.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AY307077.1, AK006225.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGCCCCGGA CGAGGAACAT CGGGGCCTTG TGTACCCTAC CTGAAGACAC AACTCACAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_083875.1 |
CDS | 87..1133 |
Translation |
Target ORF information:
Target ORF information:
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1 | ATGCCCCGGA CGAGGAACAT CGGGGCCTTG TGTACCCTAC CTGAAGACAC AACTCACAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu71491 | |
Clone ID Related Accession (Same CDS sequence) | NM_001305048.1 | |
Accession Version | NM_001305048.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-11-15 | |
Organism | Mus musculus(house mouse) | |
Product | SUN domain-containing protein 5 isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY307077.1 and AL833803.8. On Feb 19, 2015 this sequence version replaced XM_006500373.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: FJ667498.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGCCCCGGA CGAGGAACAT CGGGGCCTTG TGTACCCTAC CTGAAGACAC AACTCACAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001291977.1 |
CDS | 87..1208 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGCCCCGGA CGAGGAACAT CGGGGCCTTG TGTACCCTAC CTGAAGACAC AACTCACAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu31005 | |
Clone ID Related Accession (Same CDS sequence) | XM_006500372.1 | |
Accession Version | XM_006500372.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | SUN domain-containing protein 5 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGTGGTGG CGGCAGTGGC AGCCACTTCC TCCTCATTGA CTCCCATAGA CCTCCATCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006500435.1 |
CDS | 15..1163 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGTGGTGG CGGCAGTGGC AGCCACTTCC TCCTCATTGA CTCCCATAGA CCTCCATCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu31007 | |
Clone ID Related Accession (Same CDS sequence) | XM_006500374.1 | |
Accession Version | XM_006500374.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | SUN domain-containing protein 5 isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGTGGTGG CGGCAGTGGC AGCCACTTCC TCCTCATTGA CTCCCATAGA CCTCCATCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006500437.1 |
CDS | 15..1088 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGTGGTGG CGGCAGTGGC AGCCACTTCC TCCTCATTGA CTCCCATAGA CCTCCATCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu31008 | |
Clone ID Related Accession (Same CDS sequence) | XM_006500375.2 | |
Accession Version | XM_006500375.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | SUN domain-containing protein 5 isoform X4 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006500375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGAGCTGA TTCATACAAG CAACAGCCAT TTCAGGGAAA TGGATGACAG CATAAATGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006500438.1 |
CDS | 149..928 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGAGCTGA TTCATACAAG CAACAGCCAT TTCAGGGAAA TGGATGACAG CATAAATGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu71490 | |
Clone ID Related Accession (Same CDS sequence) | XM_011239838.1 | |
Accession Version | XM_011239838.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | SUN domain-containing protein 5 isoform X5 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGTGGTGG CGGCAGTGGC AGCCACTTCC TCCTCATTGA CTCCCATAGA CCTCCATCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_011238140.1 |
CDS | 15..782 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGTGGTGG CGGCAGTGGC AGCCACTTCC TCCTCATTGA CTCCCATAGA CCTCCATCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu88034 | |
Clone ID Related Accession (Same CDS sequence) | XM_006500372.2 | |
Accession Version | XM_006500372.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | SUN domain-containing protein 5 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500372.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCACCTT CCAGGGAATA CTCAATGGTG GTGGCGGCAG TGGCAGCCAC TTCCTCCTCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006500435.2 |
CDS | 5..1177 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGTCACCTT CCAGGGAATA CTCAATGGTG GTGGCGGCAG TGGCAGCCAC TTCCTCCTCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu88035 | |
Clone ID Related Accession (Same CDS sequence) | XM_030252189.1 | |
Accession Version | XM_030252189.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | SUN domain-containing protein 5 isoform X3 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCAGAGC CAGCCCCGGC AAACATGACC TGGCTCACCT ACCTAGCCTG CTTCCTGAGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_030108049.1 |
CDS | 122..1066 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGCAGAGC CAGCCCCGGC AAACATGACC TGGCTCACCT ACCTAGCCTG CTTCCTGAGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Mechanistic insights into acephalic spermatozoa syndrome-associated mutations in the human SUN5 gene. |
Essential role for SUN5 in anchoring sperm head to the tail. |
Dynamics of Sun5 localization during spermatogenesis in wild type and Dpy19l2 knock-out mice indicates that Sun5 is not involved in acrosome attachment to the nuclear envelope. |
SPAG4L, a novel nuclear envelope protein involved in the meiotic stage of spermatogenesis. |
SPAG4L/SPAG4L-2 are testis-specific SUN domain proteins restricted to the apical nuclear envelope of round spermatids facing the acrosome. |