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Gje1 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Gje1
Entrez Gene ID 76743
Full Name gap junction protein, epsilon 1
Synonyms AEY12,Cx23,D230044M03Rik,Gjf1,Gsfaey12
General protein information
Preferred Names

gap junction protein, epsilon 1

Names

gap junction epsilon-1 protein
connexin 23
putative gap junction protein connexin Cx43.4
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

10

10|10 A2

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_029722.2 NP_083998.1 gap junction epsilon-1 protein
NM_029722.1 NP_083998.1 gap junction epsilon-1 protein
XM_030245359.1 XP_030101219.1 gap junction epsilon-1 protein isoform X1

Pathways from BioSystems


Homologies


Bos taurus (cattle) LOC781323 XP_002690334.1
Mus musculus (house mouse) Gje1 NP_083998.1
Xenopus tropicalis (tropical clawed frog) LOC100489464 XP_002933918.1
Rattus norvegicus (Norway rat) Gje1 NP_001184021.1
Danio rerio (zebrafish) cx23 NP_001013564.1
Pan troglodytes (chimpanzee) LOC100610341 XP_003311566.2

Gene Ontology


Bibliography

Related articles in PubMed

Connexin23 deletion does not affect lens transparency.
Berthoud VM, Minogue PJ, Snabb JI, Dzhashiashvili Y, Novak LA, Zoltoski RK, Popko B, Beyer EC
Experimental eye research146283-8(2016 05)

iSyTE: integrated Systems Tool for Eye gene discovery.
Lachke SA, Ho JW, Kryukov GV, O'Connell DJ, Aboukhalil A, Bulyk ML, Park PJ, Maas RL
Investigative ophthalmology & visual science53(3)1617-27(2012 Mar)

Mutation in a novel connexin-like gene (Gjf1) in the mouse affects early lens development and causes a variable small-eye phenotype.
Puk O, L?ster J, Dalke C, Soewarto D, Fuchs H, Budde B, N?rnberg P, Wolf E, de Angelis MH, Graw J
Investigative ophthalmology & visual science49(4)1525-32(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gje1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gje1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu07375 NM_029722.2
Latest version!		 Mus musculus gap junction protein, epsilon 1 (Gje1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OMu85548 XM_030245359.1
Latest version!		 Mus musculus gap junction protein, epsilon 1 (Gje1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OMu07375 NM_029722.1 Mus musculus gap junction protein, epsilon 1 (Gje1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu07375
Clone ID Related Accession (Same CDS sequence) NM_029722.2 , NM_029722.1
Accession Version NM_029722.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-09
Organism Mus musculus(house mouse)
Product gap junction epsilon-1 protein
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC159336.13. On Jan 25, 2019 this sequence version replaced NM_029722.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK018698.1, BY730193.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849377, SAMN00849383 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGTCTCTAA ATTACATCAA GAACTTCTAT GAAGGATGTG TTAAGCCTCC AACTGTGATC 
GGCCAGTTCC ACACTCTCTT CTTCGGCTCA GTGCGGATGT TCTTCCTCGG AGTGCTGGGC 
TTTGCTGTCT ACGGGAATGA GGCGTTGCAC TTCAGCTGTG ACCCAGACAA GCGAGAGATA 
AACCTGTTCT GTTACAATCA GTTCCGGCCA ATAACTCCCC AAGTGTTCTG GGCATTGCAG 
CTAGTGATTG TCCTGCTTCC TGGAGCTATT TTCCACCTGT ATGCTGCATG CAAAAGCATC 
AATCAAGACT GCATTCTTCA GAAGCCCGTG TACACTGTGA TTTACGTCCT CTCGGTCTTG 
TTAAGAATCA GCCTGGAGGT GTTCGCATTC TGGCTTCAGA TTCACCTCTT CGGCTTCCAA 
GTGAAGCCGA TATACTTGTG TGATACTGAA TCTCTTGGTA AAAAACCAAA TATTCTAAAA 
TGCATGGTTC CAGAGCACTT TGAAAAGACT ATTTTCCTCA TTGCAATGTA CACATTTACT 
GTGATCACGA TGGTATTATG TGTTGCTGAG GTTTTTGAGA TCATATTTAG AAGATCATGT 
TTTCTCTTTA AACGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_083998.1
CDS67..684
Translation

Target ORF information:

RefSeq Version NM_029722.2
Organism Mus musculus(house mouse)
Definition Mus musculus gap junction protein, epsilon 1 (Gje1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_029722.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGTCTCTAA ATTACATCAA GAACTTCTAT GAAGGATGTG TTAAGCCTCC AACTGTGATC 
GGCCAGTTCC ACACTCTCTT CTTCGGCTCA GTGCGGATGT TCTTCCTCGG AGTGCTGGGC 
TTTGCTGTCT ACGGGAATGA GGCGTTGCAC TTCAGCTGTG ACCCAGACAA GCGAGAGATA 
AACCTGTTCT GTTACAATCA GTTCCGGCCA ATAACTCCCC AAGTGTTCTG GGCATTGCAG 
CTAGTGATTG TCCTGCTTCC TGGAGCTATT TTCCACCTGT ATGCTGCATG CAAAAGCATC 
AATCAAGACT GCATTCTTCA GAAGCCCGTG TACACTGTGA TTTACGTCCT CTCGGTCTTG 
TTAAGAATCA GCCTGGAGGT GTTCGCATTC TGGCTTCAGA TTCACCTCTT CGGCTTCCAA 
GTGAAGCCGA TATACTTGTG TGATACTGAA TCTCTTGGTA AAAAACCAAA TATTCTAAAA 
TGCATGGTTC CAGAGCACTT TGAAAAGACT ATTTTCCTCA TTGCAATGTA CACATTTACT 
GTGATCACGA TGGTATTATG TGTTGCTGAG GTTTTTGAGA TCATATTTAG AAGATCATGT 
TTTCTCTTTA AACGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu07375
Clone ID Related Accession (Same CDS sequence) NM_029722.2 , NM_029722.1
Accession Version NM_029722.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-09-14
Organism Mus musculus(house mouse)
Product gap junction epsilon-1 protein
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK018698.1. On or before Dec 3, 2009 this sequence version replaced XM_979387.1, XM_354583.3. ##Evidence-Data-START## Transcript exon combination :: AK018698.1, BY730193.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849377, SAMN00849383 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGTCTCTAA ATTACATCAA GAACTTCTAT GAAGGATGTG TTAAGCCTCC AACTGTGATC 
GGCCAGTTCC ACACTCTCTT CTTCGGCTCA GTGCGGATGT TCTTCCTCGG AGTGCTGGGC 
TTTGCTGTCT ACGGGAATGA GGCGTTGCAC TTCAGCTGTG ACCCAGACAA GCGAGAGATA 
AACCTGTTCT GTTACAATCA GTTCCGGCCA ATAACTCCCC AAGTGTTCTG GGCATTGCAG 
CTAGTGATTG TCCTGCTTCC TGGAGCTATT TTCCACCTGT ATGCTGCATG CAAAAGCATC 
AATCAAGACT GCATTCTTCA GAAGCCCGTG TACACTGTGA TTTACGTCCT CTCGGTCTTG 
TTAAGAATCA GCCTGGAGGT GTTCGCATTC TGGCTTCAGA TTCACCTCTT CGGCTTCCAA 
GTGAAGCCGA TATACTTGTG TGATACTGAA TCTCTTGGTA AAAAACCAAA TATTCTAAAA 
TGCATGGTTC CAGAGCACTT TGAAAAGACT ATTTTCCTCA TTGCAATGTA CACATTTACT 
GTGATCACGA TGGTATTATG TGTTGCTGAG GTTTTTGAGA TCATATTTAG AAGATCATGT 
TTTCTCTTTA AACGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_083998.1
CDS67..684
Translation

Target ORF information:

RefSeq Version NM_029722.1
Organism Mus musculus(house mouse)
Definition Mus musculus gap junction protein, epsilon 1 (Gje1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_029722.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGTCTCTAA ATTACATCAA GAACTTCTAT GAAGGATGTG TTAAGCCTCC AACTGTGATC 
GGCCAGTTCC ACACTCTCTT CTTCGGCTCA GTGCGGATGT TCTTCCTCGG AGTGCTGGGC 
TTTGCTGTCT ACGGGAATGA GGCGTTGCAC TTCAGCTGTG ACCCAGACAA GCGAGAGATA 
AACCTGTTCT GTTACAATCA GTTCCGGCCA ATAACTCCCC AAGTGTTCTG GGCATTGCAG 
CTAGTGATTG TCCTGCTTCC TGGAGCTATT TTCCACCTGT ATGCTGCATG CAAAAGCATC 
AATCAAGACT GCATTCTTCA GAAGCCCGTG TACACTGTGA TTTACGTCCT CTCGGTCTTG 
TTAAGAATCA GCCTGGAGGT GTTCGCATTC TGGCTTCAGA TTCACCTCTT CGGCTTCCAA 
GTGAAGCCGA TATACTTGTG TGATACTGAA TCTCTTGGTA AAAAACCAAA TATTCTAAAA 
TGCATGGTTC CAGAGCACTT TGAAAAGACT ATTTTCCTCA TTGCAATGTA CACATTTACT 
GTGATCACGA TGGTATTATG TGTTGCTGAG GTTTTTGAGA TCATATTTAG AAGATCATGT 
TTTCTCTTTA AACGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu85548
Clone ID Related Accession (Same CDS sequence) XM_030245359.1
Accession Version XM_030245359.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 651bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product gap junction epsilon-1 protein isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGAATACAT CCTCCCAATT GTCCTTTTTA AATGTTTGTT TCCAACTAAT TTTTTCCCTC 
CAAAACCAAC AGGTTAAGCC TCCAACTGTG ATCGGCCAGT TCCACACTCT CTTCTTCGGC 
TCAGTGCGGA TGTTCTTCCT CGGAGTGCTG GGCTTTGCTG TCTACGGGAA TGAGGCGTTG 
CACTTCAGCT GTGACCCAGA CAAGCGAGAG ATAAACCTGT TCTGTTACAA TCAGTTCCGG 
CCAATAACTC CCCAAGTGTT CTGGGCATTG CAGCTAGTGA TTGTCCTGCT TCCTGGAGCT 
ATTTTCCACC TGTATGCTGC ATGCAAAAGC ATCAATCAAG ACTGCATTCT TCAGAAGCCC 
GTGTACACTG TGATTTACGT CCTCTCGGTC TTGTTAAGAA TCAGCCTGGA GGTGTTCGCA 
TTCTGGCTTC AGATTCACCT CTTCGGCTTC CAAGTGAAGC CGATATACTT GTGTGATACT 
GAATCTCTTG GTAAAAAACC AAATATTCTA AAATGCATGG TTCCAGAGCA CTTTGAAAAG 
ACTATTTTCC TCATTGCAAT GTACACATTT ACTGTGATCA CGATGGTATT ATGTGTTGCT 
GAGGTTTTTG AGATCATATT TAGAAGATCA TGTTTTCTCT TTAAACGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_030101219.1
CDS27..677
Translation

Target ORF information:

RefSeq Version XM_030245359.1
Organism Mus musculus(house mouse)
Definition Mus musculus gap junction protein, epsilon 1 (Gje1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_030245359.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGAATACAT CCTCCCAATT GTCCTTTTTA AATGTTTGTT TCCAACTAAT TTTTTCCCTC 
CAAAACCAAC AGGTTAAGCC TCCAACTGTG ATCGGCCAGT TCCACACTCT CTTCTTCGGC 
TCAGTGCGGA TGTTCTTCCT CGGAGTGCTG GGCTTTGCTG TCTACGGGAA TGAGGCGTTG 
CACTTCAGCT GTGACCCAGA CAAGCGAGAG ATAAACCTGT TCTGTTACAA TCAGTTCCGG 
CCAATAACTC CCCAAGTGTT CTGGGCATTG CAGCTAGTGA TTGTCCTGCT TCCTGGAGCT 
ATTTTCCACC TGTATGCTGC ATGCAAAAGC ATCAATCAAG ACTGCATTCT TCAGAAGCCC 
GTGTACACTG TGATTTACGT CCTCTCGGTC TTGTTAAGAA TCAGCCTGGA GGTGTTCGCA 
TTCTGGCTTC AGATTCACCT CTTCGGCTTC CAAGTGAAGC CGATATACTT GTGTGATACT 
GAATCTCTTG GTAAAAAACC AAATATTCTA AAATGCATGG TTCCAGAGCA CTTTGAAAAG 
ACTATTTTCC TCATTGCAAT GTACACATTT ACTGTGATCA CGATGGTATT ATGTGTTGCT 
GAGGTTTTTG AGATCATATT TAGAAGATCA TGTTTTCTCT TTAAACGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Connexin23 deletion does not affect lens transparency.
Experimental eye research146283-8(2016 05)
Berthoud VM,Minogue PJ,Snabb JI,Dzhashiashvili Y,Novak LA,Zoltoski RK,Popko B,Beyer EC


book

iSyTE: integrated Systems Tool for Eye gene discovery.
Investigative ophthalmology & visual science53(3)1617-27(2012 Mar)
Lachke SA,Ho JW,Kryukov GV,O'Connell DJ,Aboukhalil A,Bulyk ML,Park PJ,Maas RL


book

Mutation in a novel connexin-like gene (Gjf1) in the mouse affects early lens development and causes a variable small-eye phenotype.
Investigative ophthalmology & visual science49(4)1525-32(2008 Apr)
Puk O,L?ster J,Dalke C,Soewarto D,Fuchs H,Budde B,N?rnberg P,Wolf E,de Angelis MH,Graw J