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Ckap2 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Ckap2
Entrez Gene ID 80986
Full Name cytoskeleton associated protein 2
Synonyms AI461656,AW228814,LB1
General protein information
Preferred Names

cytoskeleton associated protein 2

Names

cytoskeleton-associated protein 2
ckap2
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

8

8|8 A2

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006509226.3 XP_006509289.1 cytoskeleton-associated protein 2 isoform X1
XM_006509226.4 XP_006509289.1 cytoskeleton-associated protein 2 isoform X1
NM_001004140.2 NP_001004140.2 cytoskeleton-associated protein 2

Pathways from BioSystems


Homologies


CKAP2 XP_001084035.1
Rattus norvegicus (Norway rat) Ckap2 NP_001162610.1
Gallus gallus (chicken) CKAP2 NP_001006274.1
Danio rerio (zebrafish) si:ch211-266i6.3 XP_697274.2
Bos taurus (cattle) CKAP2 NP_001091501.1
Mus musculus (house mouse) Ckap2 NP_001004140.2
Rattus norvegicus (Norway rat) LOC691173 XP_003749023.1
Xenopus tropicalis (tropical clawed frog) ckap2 XP_002936769.2
Homo sapiens (human) CKAP2 NP_001091995.1
Pan troglodytes (chimpanzee) CKAP2 XP_001135710.1
Canis lupus familiaris (dog) CKAP2 XP_005635452.1

Gene Ontology


Bibliography

Related articles in PubMed

CKAP2 phosphorylation by CDK1/cyclinB1 is crucial for maintaining centrosome integrity.
Yoo BH, Kang DS, Park CH, Kang K, Bae CD
Experimental & molecular medicine49(7)e354(2017 Jul)

Lamin-B1 contributes to the proper timing of epicardial cell migration and function during embryonic heart development.
Tran JR, Zheng X, Zheng Y
Molecular biology of the cell27(25)3956-3963(2016 Dec)

CKAP2 is necessary to ensure the faithful spindle bipolarity in a dividing diploid hepatocyte.
Yoo BH, Park CH, Kim HJ, Kang DS, Bae CD
Biochemical and biophysical research communications473(4)886-893(2016 05)

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Ray JP, Staron MM, Shyer JA, Ho PC, Marshall HD, Gray SM, Laidlaw BJ, Araki K, Ahmed R, Kaech SM, Craft J
Immunity43(4)690-702(2015 Oct)

Angiogenesis QTL on Mouse Chromosome 8 Colocalizes with Differential ?-Defensin Expression.
Smith J, Liu F, Beyer B, Morales K, Reilly A, Cole R, Herron BJ
Journal of biomolecular techniques : JBT26(2)45-53(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we found that Lb1-null epicardial cells have a delayed nuclear morphology change in vivo, suggesting that Lb1 facilitates morphological changes associated with migration. These findings suggest that Lb1 contributes to nuclear shape maintenance and migration of epicardial cells
Title: Lamin-B1 contributes to the proper timing of epicardial cell migration and function during embryonic heart development.

These data suggest a physiological role of CKAP2 in the formation of spindle bipolarity, which is necessary for maintaining chromosomal stability.
Title: CKAP2 is necessary to ensure the faithful spindle bipolarity in a dividing diploid hepatocyte.

Our present findings demonstrate that TMAP/CKAP2 is essential for proper chromosome segregation and for maintaining genomic stability.
Title: TMAP/CKAP2 is essential for proper chromosome segregation.

The cellular functions of TMAP/CKAP2 might be regulated by timely phosphorylation and dephosphorylation during the course of mitosis.
Title: Transient phosphorylation of tumor associated microtubule associated protein (TMAP)/cytoskeleton associated protein 2 (CKAP2) at Thr-596 during early phases of mitosis.

Data suggest the existence of a functional positive feedback loop in which Ckap2 activates the G1 tetraploidy checkpoint and prevents aneuploidy.
Title: Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.

The following Ckap2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ckap2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu10590 NM_001004140.2
Latest version!		 Mus musculus cytoskeleton associated protein 2 (Ckap2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OMu40710 XM_006509226.4
Latest version!		 Mus musculus cytoskeleton associated protein 2 (Ckap2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OMu40710 XM_006509226.3 Mus musculus cytoskeleton associated protein 2 (Ckap2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu40710
Clone ID Related Accession (Same CDS sequence) XM_006509226.3 , XM_006509226.4
Accession Version XM_006509226.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1794bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product cytoskeleton-associated protein 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509226.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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ATGATAACAT CTGAGGATCG GGTCCAAGAA GGGAAAAAAG TTGTGAAACT CAAAACAGAA 
GTGGCTGATA AAGAAAATAT TGAAAGTACA GTTGAGAAAA ACTGCATTCC ATTAAAAGCT 
GGAGAAGTAA CCAGCTCAGA AATACATAAT TCAAAGGATA ATGTTCAAGC TGTGCAGTTA 
TTATCTACAA GAGATGATTT GCCAGGTCAG ACTGTGACAT TAGACCCGGC ATGTCACCAT 
AAAGACAATA AAAAGATGCA AATGACTGCA GAGAAGCCCA AACAAGACAG TAATGTGTCT 
AAAAAGCGAG TGCTTGGCTA TTATCATGGC CAGATTGTTC AGTCTAAGAT TAATTCATTT 
AGAAAGCTTC CCAGTGTCAA AGGCGAGAGT CTTACAACAA CCAAGAAACT TCCCACTACT 
GTGTCTAAAG CCATGAAGGC TCAGAGTGAG CCTGCGAACA CCGTCAGCGT AAAAGCCTCA 
ACTACAGCTG CTGCCACCAA GTTTGCAGAC GCTAAACCTG TGAGCACTGC GTCGAAGGAC 
ACACTTGTGC GACCACCTAT CCGAAGCCTC CACAGTAGTT CCCACGGAGC TGCGAAACAG 
GGCCTCAGCA GGCCGCTTGC CAATGTTACA GTCAGAAAAG GGATGCTTGA CAAGGAGTCA 
CATCGATCAG AACCAGTTGT GTCCGTTGTC AAAGCCGGTT CTTCTCAGGC CCCATCAAGA 
AGCATAGCAT CTAAAGATGC AGCCAGGACT GATTCGTCTA ATACCAGACT GATGGTAAAG 
CCAAAAGATA CTGACCAGCG CAGATACACG ATTGCAGGAG CAAGTGTTCA TAGATCAGCT 
CAGCTCAAGG ACACCACCGC AGAGAGGAAA GCTCAGATGA CGGAGTGGAG AACTGGCAAA 
GGAAAGGGGC TAAAACGACC TCCTCATTCA GTAGTCACCC AGGCTGAGCC AAAGGGACAA 
AGTGAAAACC CAGTTGGGTC CTTTTGGACT ACCATGGCAG AAGAAGATGA GCAGAGATTA 
TTTACTGAAA AAGTAAACAA AACTATTTCT GAATGCCTGA ACCTTATTAA TGAGGGATGC 
CCAAAAGAAG AAATTTTGGC TACATTGAAT GACCTGATTC ATAATATTCC AGATGCCAAA 
AAACTTGTTA AATATTGGAT ATGTCTTGTA CGTATTGAAC CAATCACAAG TCCTATTGAA 
AATATTATCT CAATCTATGA GAAGGCCATC CTGGCAGGGG CTCAGCCTAT TGAAGAGATG 
CGTCATATAA TCATAGATAT TCTAACAACG AAGAGTCAAG AAAAAGTCAA TTTGGGAGAA 
AATATTGAGG AGGCTCATGC AACCAAGGAA CCTATCCAAG AAGTAAATGC TGATGCTAAT 
GTAGGGTCAG GAAAACCAGG AGAGGAGAAT GAACATCATG GCAAAGTTGA GGTGTATGAA 
GATGATCAAG ACAACAAAAT AAAAGATCCG GATTTAACAA CTCCGGACTC AAAAACTGAA 
GCAGGTTGCA TAATTCGATA TAATGTATCT TCTACACCTC GGCTGCAAAG TATGAAAAAG 
ATGCAACATG ATAAAAATTC CACACTTAAA GAGCTAAAGT TCCTGACACC AGTGAGACGC 
TCACGGCGCA TTCAAGACAA GACTTCACGA CTGCCAGCCA TGTTAAAAGA CCACGACCCT 
TCTGTGTCTT CCCTGGAGCA GTTGTCTGAG TTGGGAGGGG ATGCCTTTGT CTGCCGCCCT 
AATGCAGCAC TGTGCCCTCT GTTCTTTGAG ACCGACGTAG CGGAAGAGGA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006509289.1
CDS238..2031
Translation

Target ORF information:

RefSeq Version XM_006509226.3
Organism Mus musculus(house mouse)
Definition Mus musculus cytoskeleton associated protein 2 (Ckap2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006509226.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGATAACAT CTGAGGATCG GGTCCAAGAA GGGAAAAAAG TTGTGAAACT CAAAACAGAA 
GTGGCTGATA AAGAAAATAT TGAAAGTACA GTTGAGAAAA ACTGCATTCC ATTAAAAGCT 
GGAGAAGTAA CCAGCTCAGA AATACATAAT TCAAAGGATA ATGTTCAAGC TGTGCAGTTA 
TTATCTACAA GAGATGATTT GCCAGGTCAG ACTGTGACAT TAGACCCGGC ATGTCACCAT 
AAAGACAATA AAAAGATGCA AATGACTGCA GAGAAGCCCA AACAAGACAG TAATGTGTCT 
AAAAAGCGAG TGCTTGGCTA TTATCATGGC CAGATTGTTC AGTCTAAGAT TAATTCATTT 
AGAAAGCTTC CCAGTGTCAA AGGCGAGAGT CTTACAACAA CCAAGAAACT TCCCACTACT 
GTGTCTAAAG CCATGAAGGC TCAGAGTGAG CCTGCGAACA CCGTCAGCGT AAAAGCCTCA 
ACTACAGCTG CTGCCACCAA GTTTGCAGAC GCTAAACCTG TGAGCACTGC GTCGAAGGAC 
ACACTTGTGC GACCACCTAT CCGAAGCCTC CACAGTAGTT CCCACGGAGC TGCGAAACAG 
GGCCTCAGCA GGCCGCTTGC CAATGTTACA GTCAGAAAAG GGATGCTTGA CAAGGAGTCA 
CATCGATCAG AACCAGTTGT GTCCGTTGTC AAAGCCGGTT CTTCTCAGGC CCCATCAAGA 
AGCATAGCAT CTAAAGATGC AGCCAGGACT GATTCGTCTA ATACCAGACT GATGGTAAAG 
CCAAAAGATA CTGACCAGCG CAGATACACG ATTGCAGGAG CAAGTGTTCA TAGATCAGCT 
CAGCTCAAGG ACACCACCGC AGAGAGGAAA GCTCAGATGA CGGAGTGGAG AACTGGCAAA 
GGAAAGGGGC TAAAACGACC TCCTCATTCA GTAGTCACCC AGGCTGAGCC AAAGGGACAA 
AGTGAAAACC CAGTTGGGTC CTTTTGGACT ACCATGGCAG AAGAAGATGA GCAGAGATTA 
TTTACTGAAA AAGTAAACAA AACTATTTCT GAATGCCTGA ACCTTATTAA TGAGGGATGC 
CCAAAAGAAG AAATTTTGGC TACATTGAAT GACCTGATTC ATAATATTCC AGATGCCAAA 
AAACTTGTTA AATATTGGAT ATGTCTTGTA CGTATTGAAC CAATCACAAG TCCTATTGAA 
AATATTATCT CAATCTATGA GAAGGCCATC CTGGCAGGGG CTCAGCCTAT TGAAGAGATG 
CGTCATATAA TCATAGATAT TCTAACAACG AAGAGTCAAG AAAAAGTCAA TTTGGGAGAA 
AATATTGAGG AGGCTCATGC AACCAAGGAA CCTATCCAAG AAGTAAATGC TGATGCTAAT 
GTAGGGTCAG GAAAACCAGG AGAGGAGAAT GAACATCATG GCAAAGTTGA GGTGTATGAA 
GATGATCAAG ACAACAAAAT AAAAGATCCG GATTTAACAA CTCCGGACTC AAAAACTGAA 
GCAGGTTGCA TAATTCGATA TAATGTATCT TCTACACCTC GGCTGCAAAG TATGAAAAAG 
ATGCAACATG ATAAAAATTC CACACTTAAA GAGCTAAAGT TCCTGACACC AGTGAGACGC 
TCACGGCGCA TTCAAGACAA GACTTCACGA CTGCCAGCCA TGTTAAAAGA CCACGACCCT 
TCTGTGTCTT CCCTGGAGCA GTTGTCTGAG TTGGGAGGGG ATGCCTTTGT CTGCCGCCCT 
AATGCAGCAC TGTGCCCTCT GTTCTTTGAG ACCGACGTAG CGGAAGAGGA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu10590
Clone ID Related Accession (Same CDS sequence) NM_001004140.2
Accession Version NM_001004140.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-21
Organism Mus musculus(house mouse)
Product cytoskeleton-associated protein 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117665.6, AK132463.1 and BM217153.2. On Nov 20, 2009 this sequence version replaced NM_001004140.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY692438.1, AK132463.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## 

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ATGGCAGAGT CCAGGAAACG CTTCCTTGGC AGGGCTGCGA GGAATCCCTT GCCGGTGACC 
CGGGACCTGC AGCTGCCCCC GACCAGGCGA GACCAGCCGG CCTTCCGAGA ACAAAGAAAA 
CAAAAACTCA AGGAATATCT GTTAATACGA AAAACAGTGT TTCCATACAA ACAAGAAAAT 
CAGATATCCA GAGATCAGAA AATGATAACA TCTGAGGATC GGGTCCAAGA AGGGAAAAAA 
GTTGTGAAAC TCAAAACAGA AGTGGCTGAT AAAGAAAATA TTGAAAGTAC AGTTGAGAAA 
AACTGCATTC CATTAAAAGC TGGAGAAGTA ACCAGCTCAG AAATACATAA TTCAAAGGAT 
AATGTTCAAG CTGTGCAGTT ATTATCTACA AGAGATGATT TGCCAGGTCA GACTGTGACA 
TTAGACCCGG CATGTCACCA TAAAGACAAT AAAAAGATGC AAATGACTGC AGAGAAGCCC 
AAACAAGACA GTAATGTGTC TAAAAAGCGA GTGCTTGGCT ATTATCATGG CCAGATTGTT 
CAGTCTAAGA TTAATTCATT TAGAAAGCTT CCCAGTGTCA AAGGCGAGAG TCTTACAACA 
ACCAAGAAAC TTCCCACTAC TGTGTCTAAA GCCATGAAGG CTCAGAGTGA GCCTGCGAAC 
ACCGTCAGCG TAAAAGCCTC AACTACAGCT GCTGCCACCA AGTTTGCAGA CGCTAAACCT 
GTGAGCACTG CGTCGAAGGA CACACTTGTG CGACCACCTA TCCGAAGCCT CCACAGTAGT 
TCCCACGGAG CTGCGAAACA GGGCCTCAGC AGGCCGCTTG CCAATGTTAC AGTCAGAAAA 
GGGATGCTTG ACAAGGAGTC ACATCGATCA GAACCAGTTG TGTCCGTTGT CAAAGCCGGT 
TCTTCTCAGG CCCCATCAAG AAGCATAGCA TCTAAAGATG CAGCCAGGAC TGATTCGTCT 
AATACCAGAC TGATGGTAAA GCCAAAAGAT ACTGACCAGC GCAGATACAC GATTGCAGGA 
GCAAGTGTTC ATAGATCAGC TCAGCTCAAG GACACCACCG CAGAGAGGAA AGCTCAGATG 
ACGGAGTGGA GAACTGGCAA AGGAAAGGGG CTAAAACGAC CTCCTCATTC AGTAGTCACC 
CAGGCTGAGC CAAAGGGACA AAGTGAAAAC CCAGTTGGGT CCTTTTGGAC TACCATGGCA 
GAAGAAGATG AGCAGAGATT ATTTACTGAA AAAGTAAACA AAACTATTTC TGAATGCCTG 
AACCTTATTA ATGAGGGATG CCCAAAAGAA GAAATTTTGG CTACATTGAA TGACCTGATT 
CATAATATTC CAGATGCCAA AAAACTTGTT AAATATTGGA TATGTCTTGT ACGTATTGAA 
CCAATCACAA GTCCTATTGA AAATATTATC TCAATCTATG AGAAGGCCAT CCTGGCAGGG 
GCTCAGCCTA TTGAAGAGAT GCGTCATATA ATCATAGATA TTCTAACAAC GAAGAGTCAA 
GAAAAAGTCA ATTTGGGAGA AAATATTGAG GAGGCTCATG CAACCAAGGA ACCTATCCAA 
GAAGTAAATG CTGATGCTAA TGTAGGGTCA GGAAAACCAG GAGAGGAGAA TGAACATCAT 
GGCAAAGTTG AGGTGTATGA AGATGATCAA GACAACAAAA TAAAAGATCC GGATTTAACA 
ACTCCGGACT CAAAAACTGA AGCAGGTTGC ATAATTCGAT ATAATGTATC TTCTACACCT 
CGGCTGCAAA GTATGAAAAA GATGCAACAT GATAAAAATT CCACACTTAA AGAGCTAAAG 
TTCCTGACAC CAGTGAGACG CTCACGGCGC ATTCAAGACA AGACTTCACG ACTGCCAGCC 
ATGTTAAAAG ACCACGACCC TTCTGTGTCT TCCCTGGAGC AGTTGTCTGA GTTGGGAGGG 
GATGCCTTTG TCTGCCGCCC TAATGCAGCA CTGTGCCCTC TGTTCTTTGA GACCGACGTA 
GCGGAAGAGG AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001004140.2
CDS28..2022
Translation

Target ORF information:

RefSeq Version NM_001004140.2
Organism Mus musculus(house mouse)
Definition Mus musculus cytoskeleton associated protein 2 (Ckap2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001004140.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCAGAGT CCAGGAAACG CTTCCTTGGC AGGGCTGCGA GGAATCCCTT GCCGGTGACC 
CGGGACCTGC AGCTGCCCCC GACCAGGCGA GACCAGCCGG CCTTCCGAGA ACAAAGAAAA 
CAAAAACTCA AGGAATATCT GTTAATACGA AAAACAGTGT TTCCATACAA ACAAGAAAAT 
CAGATATCCA GAGATCAGAA AATGATAACA TCTGAGGATC GGGTCCAAGA AGGGAAAAAA 
GTTGTGAAAC TCAAAACAGA AGTGGCTGAT AAAGAAAATA TTGAAAGTAC AGTTGAGAAA 
AACTGCATTC CATTAAAAGC TGGAGAAGTA ACCAGCTCAG AAATACATAA TTCAAAGGAT 
AATGTTCAAG CTGTGCAGTT ATTATCTACA AGAGATGATT TGCCAGGTCA GACTGTGACA 
TTAGACCCGG CATGTCACCA TAAAGACAAT AAAAAGATGC AAATGACTGC AGAGAAGCCC 
AAACAAGACA GTAATGTGTC TAAAAAGCGA GTGCTTGGCT ATTATCATGG CCAGATTGTT 
CAGTCTAAGA TTAATTCATT TAGAAAGCTT CCCAGTGTCA AAGGCGAGAG TCTTACAACA 
ACCAAGAAAC TTCCCACTAC TGTGTCTAAA GCCATGAAGG CTCAGAGTGA GCCTGCGAAC 
ACCGTCAGCG TAAAAGCCTC AACTACAGCT GCTGCCACCA AGTTTGCAGA CGCTAAACCT 
GTGAGCACTG CGTCGAAGGA CACACTTGTG CGACCACCTA TCCGAAGCCT CCACAGTAGT 
TCCCACGGAG CTGCGAAACA GGGCCTCAGC AGGCCGCTTG CCAATGTTAC AGTCAGAAAA 
GGGATGCTTG ACAAGGAGTC ACATCGATCA GAACCAGTTG TGTCCGTTGT CAAAGCCGGT 
TCTTCTCAGG CCCCATCAAG AAGCATAGCA TCTAAAGATG CAGCCAGGAC TGATTCGTCT 
AATACCAGAC TGATGGTAAA GCCAAAAGAT ACTGACCAGC GCAGATACAC GATTGCAGGA 
GCAAGTGTTC ATAGATCAGC TCAGCTCAAG GACACCACCG CAGAGAGGAA AGCTCAGATG 
ACGGAGTGGA GAACTGGCAA AGGAAAGGGG CTAAAACGAC CTCCTCATTC AGTAGTCACC 
CAGGCTGAGC CAAAGGGACA AAGTGAAAAC CCAGTTGGGT CCTTTTGGAC TACCATGGCA 
GAAGAAGATG AGCAGAGATT ATTTACTGAA AAAGTAAACA AAACTATTTC TGAATGCCTG 
AACCTTATTA ATGAGGGATG CCCAAAAGAA GAAATTTTGG CTACATTGAA TGACCTGATT 
CATAATATTC CAGATGCCAA AAAACTTGTT AAATATTGGA TATGTCTTGT ACGTATTGAA 
CCAATCACAA GTCCTATTGA AAATATTATC TCAATCTATG AGAAGGCCAT CCTGGCAGGG 
GCTCAGCCTA TTGAAGAGAT GCGTCATATA ATCATAGATA TTCTAACAAC GAAGAGTCAA 
GAAAAAGTCA ATTTGGGAGA AAATATTGAG GAGGCTCATG CAACCAAGGA ACCTATCCAA 
GAAGTAAATG CTGATGCTAA TGTAGGGTCA GGAAAACCAG GAGAGGAGAA TGAACATCAT 
GGCAAAGTTG AGGTGTATGA AGATGATCAA GACAACAAAA TAAAAGATCC GGATTTAACA 
ACTCCGGACT CAAAAACTGA AGCAGGTTGC ATAATTCGAT ATAATGTATC TTCTACACCT 
CGGCTGCAAA GTATGAAAAA GATGCAACAT GATAAAAATT CCACACTTAA AGAGCTAAAG 
TTCCTGACAC CAGTGAGACG CTCACGGCGC ATTCAAGACA AGACTTCACG ACTGCCAGCC 
ATGTTAAAAG ACCACGACCC TTCTGTGTCT TCCCTGGAGC AGTTGTCTGA GTTGGGAGGG 
GATGCCTTTG TCTGCCGCCC TAATGCAGCA CTGTGCCCTC TGTTCTTTGA GACCGACGTA 
GCGGAAGAGG AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu40710
Clone ID Related Accession (Same CDS sequence) XM_006509226.3 , XM_006509226.4
Accession Version XM_006509226.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1794bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product cytoskeleton-associated protein 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509226.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGATAACAT CTGAGGATCG GGTCCAAGAA GGGAAAAAAG TTGTGAAACT CAAAACAGAA 
GTGGCTGATA AAGAAAATAT TGAAAGTACA GTTGAGAAAA ACTGCATTCC ATTAAAAGCT 
GGAGAAGTAA CCAGCTCAGA AATACATAAT TCAAAGGATA ATGTTCAAGC TGTGCAGTTA 
TTATCTACAA GAGATGATTT GCCAGGTCAG ACTGTGACAT TAGACCCGGC ATGTCACCAT 
AAAGACAATA AAAAGATGCA AATGACTGCA GAGAAGCCCA AACAAGACAG TAATGTGTCT 
AAAAAGCGAG TGCTTGGCTA TTATCATGGC CAGATTGTTC AGTCTAAGAT TAATTCATTT 
AGAAAGCTTC CCAGTGTCAA AGGCGAGAGT CTTACAACAA CCAAGAAACT TCCCACTACT 
GTGTCTAAAG CCATGAAGGC TCAGAGTGAG CCTGCGAACA CCGTCAGCGT AAAAGCCTCA 
ACTACAGCTG CTGCCACCAA GTTTGCAGAC GCTAAACCTG TGAGCACTGC GTCGAAGGAC 
ACACTTGTGC GACCACCTAT CCGAAGCCTC CACAGTAGTT CCCACGGAGC TGCGAAACAG 
GGCCTCAGCA GGCCGCTTGC CAATGTTACA GTCAGAAAAG GGATGCTTGA CAAGGAGTCA 
CATCGATCAG AACCAGTTGT GTCCGTTGTC AAAGCCGGTT CTTCTCAGGC CCCATCAAGA 
AGCATAGCAT CTAAAGATGC AGCCAGGACT GATTCGTCTA ATACCAGACT GATGGTAAAG 
CCAAAAGATA CTGACCAGCG CAGATACACG ATTGCAGGAG CAAGTGTTCA TAGATCAGCT 
CAGCTCAAGG ACACCACCGC AGAGAGGAAA GCTCAGATGA CGGAGTGGAG AACTGGCAAA 
GGAAAGGGGC TAAAACGACC TCCTCATTCA GTAGTCACCC AGGCTGAGCC AAAGGGACAA 
AGTGAAAACC CAGTTGGGTC CTTTTGGACT ACCATGGCAG AAGAAGATGA GCAGAGATTA 
TTTACTGAAA AAGTAAACAA AACTATTTCT GAATGCCTGA ACCTTATTAA TGAGGGATGC 
CCAAAAGAAG AAATTTTGGC TACATTGAAT GACCTGATTC ATAATATTCC AGATGCCAAA 
AAACTTGTTA AATATTGGAT ATGTCTTGTA CGTATTGAAC CAATCACAAG TCCTATTGAA 
AATATTATCT CAATCTATGA GAAGGCCATC CTGGCAGGGG CTCAGCCTAT TGAAGAGATG 
CGTCATATAA TCATAGATAT TCTAACAACG AAGAGTCAAG AAAAAGTCAA TTTGGGAGAA 
AATATTGAGG AGGCTCATGC AACCAAGGAA CCTATCCAAG AAGTAAATGC TGATGCTAAT 
GTAGGGTCAG GAAAACCAGG AGAGGAGAAT GAACATCATG GCAAAGTTGA GGTGTATGAA 
GATGATCAAG ACAACAAAAT AAAAGATCCG GATTTAACAA CTCCGGACTC AAAAACTGAA 
GCAGGTTGCA TAATTCGATA TAATGTATCT TCTACACCTC GGCTGCAAAG TATGAAAAAG 
ATGCAACATG ATAAAAATTC CACACTTAAA GAGCTAAAGT TCCTGACACC AGTGAGACGC 
TCACGGCGCA TTCAAGACAA GACTTCACGA CTGCCAGCCA TGTTAAAAGA CCACGACCCT 
TCTGTGTCTT CCCTGGAGCA GTTGTCTGAG TTGGGAGGGG ATGCCTTTGT CTGCCGCCCT 
AATGCAGCAC TGTGCCCTCT GTTCTTTGAG ACCGACGTAG CGGAAGAGGA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006509289.1
CDS412..2205
Translation

Target ORF information:

RefSeq Version XM_006509226.4
Organism Mus musculus(house mouse)
Definition Mus musculus cytoskeleton associated protein 2 (Ckap2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006509226.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGATAACAT CTGAGGATCG GGTCCAAGAA GGGAAAAAAG TTGTGAAACT CAAAACAGAA 
GTGGCTGATA AAGAAAATAT TGAAAGTACA GTTGAGAAAA ACTGCATTCC ATTAAAAGCT 
GGAGAAGTAA CCAGCTCAGA AATACATAAT TCAAAGGATA ATGTTCAAGC TGTGCAGTTA 
TTATCTACAA GAGATGATTT GCCAGGTCAG ACTGTGACAT TAGACCCGGC ATGTCACCAT 
AAAGACAATA AAAAGATGCA AATGACTGCA GAGAAGCCCA AACAAGACAG TAATGTGTCT 
AAAAAGCGAG TGCTTGGCTA TTATCATGGC CAGATTGTTC AGTCTAAGAT TAATTCATTT 
AGAAAGCTTC CCAGTGTCAA AGGCGAGAGT CTTACAACAA CCAAGAAACT TCCCACTACT 
GTGTCTAAAG CCATGAAGGC TCAGAGTGAG CCTGCGAACA CCGTCAGCGT AAAAGCCTCA 
ACTACAGCTG CTGCCACCAA GTTTGCAGAC GCTAAACCTG TGAGCACTGC GTCGAAGGAC 
ACACTTGTGC GACCACCTAT CCGAAGCCTC CACAGTAGTT CCCACGGAGC TGCGAAACAG 
GGCCTCAGCA GGCCGCTTGC CAATGTTACA GTCAGAAAAG GGATGCTTGA CAAGGAGTCA 
CATCGATCAG AACCAGTTGT GTCCGTTGTC AAAGCCGGTT CTTCTCAGGC CCCATCAAGA 
AGCATAGCAT CTAAAGATGC AGCCAGGACT GATTCGTCTA ATACCAGACT GATGGTAAAG 
CCAAAAGATA CTGACCAGCG CAGATACACG ATTGCAGGAG CAAGTGTTCA TAGATCAGCT 
CAGCTCAAGG ACACCACCGC AGAGAGGAAA GCTCAGATGA CGGAGTGGAG AACTGGCAAA 
GGAAAGGGGC TAAAACGACC TCCTCATTCA GTAGTCACCC AGGCTGAGCC AAAGGGACAA 
AGTGAAAACC CAGTTGGGTC CTTTTGGACT ACCATGGCAG AAGAAGATGA GCAGAGATTA 
TTTACTGAAA AAGTAAACAA AACTATTTCT GAATGCCTGA ACCTTATTAA TGAGGGATGC 
CCAAAAGAAG AAATTTTGGC TACATTGAAT GACCTGATTC ATAATATTCC AGATGCCAAA 
AAACTTGTTA AATATTGGAT ATGTCTTGTA CGTATTGAAC CAATCACAAG TCCTATTGAA 
AATATTATCT CAATCTATGA GAAGGCCATC CTGGCAGGGG CTCAGCCTAT TGAAGAGATG 
CGTCATATAA TCATAGATAT TCTAACAACG AAGAGTCAAG AAAAAGTCAA TTTGGGAGAA 
AATATTGAGG AGGCTCATGC AACCAAGGAA CCTATCCAAG AAGTAAATGC TGATGCTAAT 
GTAGGGTCAG GAAAACCAGG AGAGGAGAAT GAACATCATG GCAAAGTTGA GGTGTATGAA 
GATGATCAAG ACAACAAAAT AAAAGATCCG GATTTAACAA CTCCGGACTC AAAAACTGAA 
GCAGGTTGCA TAATTCGATA TAATGTATCT TCTACACCTC GGCTGCAAAG TATGAAAAAG 
ATGCAACATG ATAAAAATTC CACACTTAAA GAGCTAAAGT TCCTGACACC AGTGAGACGC 
TCACGGCGCA TTCAAGACAA GACTTCACGA CTGCCAGCCA TGTTAAAAGA CCACGACCCT 
TCTGTGTCTT CCCTGGAGCA GTTGTCTGAG TTGGGAGGGG ATGCCTTTGT CTGCCGCCCT 
AATGCAGCAC TGTGCCCTCT GTTCTTTGAG ACCGACGTAG CGGAAGAGGA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

CKAP2 phosphorylation by CDK1/cyclinB1 is crucial for maintaining centrosome integrity.
Experimental & molecular medicine49(7)e354(2017 Jul)
Yoo BH,Kang DS,Park CH,Kang K,Bae CD


book

Lamin-B1 contributes to the proper timing of epicardial cell migration and function during embryonic heart development.
Molecular biology of the cell27(25)3956-3963(2016 Dec)
Tran JR,Zheng X,Zheng Y


book

CKAP2 is necessary to ensure the faithful spindle bipolarity in a dividing diploid hepatocyte.
Biochemical and biophysical research communications473(4)886-893(2016 05)
Yoo BH,Park CH,Kim HJ,Kang DS,Bae CD


book

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Immunity43(4)690-702(2015 Oct)
Ray JP,Staron MM,Shyer JA,Ho PC,Marshall HD,Gray SM,Laidlaw BJ,Araki K,Ahmed R,Kaech SM,Craft J


book

Angiogenesis QTL on Mouse Chromosome 8 Colocalizes with Differential ?-Defensin Expression.
Journal of biomolecular techniques : JBT26(2)45-53(2015 Jul)
Smith J,Liu F,Beyer B,Morales K,Reilly A,Cole R,Herron BJ